AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0173350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0173350 MtrunA17_Chr1g0193071 86.719 128 11 2 1 128 1 122 1.76e-70 207
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0173350 AT5G26749.1 40.136 147 69 2 1 128 1 147 5.47e-32 110
Msa0173350 AT5G26749.2 35.542 166 69 3 1 128 1 166 1.87e-27 99.8

Find 86 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACCTGGCATTCTTGTTAT+TGG 0.166998 1_4:+58585790 None:intergenic
TGCTGCTGCTGTTGTGTATT+GGG 0.205632 1_4:+58585814 None:intergenic
GCTTCGACAACCTCTGTTCT+AGG 0.236330 1_4:-58586486 Msa0173350:five_prime_UTR
TACCGGCAGAGTGGCGTTTC+CGG 0.248184 1_4:+58586072 None:intergenic
AGGCAGGCATACAAGGAAAA+TGG 0.254879 1_4:+58586537 None:intergenic
CCTCAACTCTACTACAAATA+TGG 0.258437 1_4:+58586181 None:intergenic
TCCTTTCTACGCTCACATTA+AGG 0.273416 1_4:-58586404 Msa0173350:intron
AGAGTGGCGTTTCCGGTCTA+AGG 0.283984 1_4:+58586079 None:intergenic
TCATTATAGCCATAAAATAA+TGG 0.316834 1_4:+58585521 None:intergenic
TTGCTGCTGCTGTTGTGTAT+TGG 0.317673 1_4:+58585813 None:intergenic
GGAATAGAGTTCTGGTGTTC+GGG 0.348282 1_4:+58586005 None:intergenic
AGATTTGTGCACATTACCTT+TGG 0.358949 1_4:-58585560 Msa0173350:three_prime_UTR
TGGAATAGAGTTCTGGTGTT+CGG 0.377509 1_4:+58586004 None:intergenic
ATCTATCCCCAGTCGACAAA+TGG 0.397656 1_4:+58585579 None:intergenic
AGGCGTGGGTGTGGTGAGGA+GGG 0.402639 1_4:-58586161 Msa0173350:five_prime_UTR
GAGGCGTGGGTGTGGTGAGG+AGG 0.413445 1_4:-58586162 Msa0173350:five_prime_UTR
GCGCAGAACGGAGGCAGTCC+TGG 0.424429 1_4:+58586444 None:intergenic
ATGGTGGTGGAATAGAGTTC+TGG 0.430967 1_4:+58585997 None:intergenic
GGAATCGTACCAACGAGCTT+TGG 0.434321 1_4:+58586034 None:intergenic
ATAAAATAATGGTTGCATAC+AGG 0.435792 1_4:+58585532 None:intergenic
GATTTGTGCACATTACCTTT+GGG 0.444503 1_4:-58585559 Msa0173350:three_prime_UTR
ATGTGAGCGTAGAAAGGAAT+TGG 0.444915 1_4:+58586409 None:intergenic
CAACCTCCCATTTGTCGACT+GGG 0.457123 1_4:-58585586 Msa0173350:CDS
GTCACTGCAGCCTCCGCCTG+AGG 0.458331 1_4:-58585619 Msa0173350:CDS
TCACTGCAGCCTCCGCCTGA+GGG 0.463350 1_4:-58585618 Msa0173350:CDS
GTTCTGGTGTTCGGGTTTGA+AGG 0.464430 1_4:+58586013 None:intergenic
ACGCAGAAGGGTTGATTAGA+TGG 0.466779 1_4:+58585978 None:intergenic
GGCAGGCATACAAGGAAAAT+GGG 0.474158 1_4:+58586538 None:intergenic
GGCTTGTTTGTGTTGAATAC+CGG 0.496651 1_4:+58586055 None:intergenic
GGCGTGGGTGTGGTGAGGAG+GGG 0.501306 1_4:-58586160 Msa0173350:five_prime_UTR
TCTATCCCCAGTCGACAAAT+GGG 0.506048 1_4:+58585580 None:intergenic
TACTGGTTATTACAACTACT+AGG 0.507008 1_4:-58585452 Msa0173350:three_prime_UTR
GATACCATGGGAGTGTCGTT+CGG 0.508317 1_4:-58585654 Msa0173350:CDS
GACCGGAAACGCCACTCTGC+CGG 0.512557 1_4:-58586074 Msa0173350:CDS
AGATATGTATAGAGGAGAGA+TGG 0.512762 1_4:+58585382 None:intergenic
CCAACCTCCCATTTGTCGAC+TGG 0.514767 1_4:-58585587 Msa0173350:CDS
TAAAATAATGGTTGCATACA+GGG 0.515469 1_4:+58585533 None:intergenic
TCAGGCGGAGGCTGCAGTGA+CGG 0.518687 1_4:+58585621 None:intergenic
CTGCAGCCTCCGCCTGAGGG+TGG 0.519587 1_4:-58585615 Msa0173350:CDS
AAGGGTTGATTAGATGGTGG+TGG 0.521880 1_4:+58585984 None:intergenic
ACCTTAATGTGAGCGTAGAA+AGG 0.522329 1_4:+58586403 None:intergenic
CCGGCGCGACACCGTTCTCC+AGG 0.528215 1_4:-58586462 Msa0173350:five_prime_UTR
TTCAAGATACACCCTTAGAC+CGG 0.529755 1_4:-58586091 Msa0173350:CDS
CAGAGGTTGTCGAAGCAAAA+CGG 0.531061 1_4:+58586493 None:intergenic
AACATACTAATAGTTCTGAT+AGG 0.533532 1_4:-58585498 Msa0173350:three_prime_UTR
GAGTATCCACCCTCAGGCGG+AGG 0.540027 1_4:+58585609 None:intergenic
AGGTTGGAGTATCCACCCTC+AGG 0.543831 1_4:+58585603 None:intergenic
AGACACAAGGCAGGCATACA+AGG 0.545292 1_4:+58586530 None:intergenic
ATATATGTAGGGAATATAGT+AGG 0.551592 1_4:-58585678 Msa0173350:intron
GACAACCTCTGTTCTAGGTC+CGG 0.553667 1_4:-58586481 Msa0173350:five_prime_UTR
GTAGAGTTGAGGCGTGGGTG+TGG 0.557491 1_4:-58586170 Msa0173350:five_prime_UTR
GTTGAGGCGTGGGTGTGGTG+AGG 0.560632 1_4:-58586165 Msa0173350:five_prime_UTR
CCAGTCGACAAATGGGAGGT+TGG 0.561835 1_4:+58585587 None:intergenic
AACTTTCGATCATAAAACAA+AGG 0.564239 1_4:+58586565 None:intergenic
ATAGTACCGTGTTGACGAAG+TGG 0.572113 1_4:+58585955 None:intergenic
CAACCAATAACAAGAATGCC+AGG 0.578455 1_4:-58585793 Msa0173350:intron
TTGTAGTAGAGTTGAGGCGT+GGG 0.580503 1_4:-58586175 Msa0173350:five_prime_UTR
GAGTGGCGTTTCCGGTCTAA+GGG 0.584406 1_4:+58586080 None:intergenic
TACAGCGTCGTCGCGCAGAA+CGG 0.586040 1_4:+58586432 None:intergenic
GTCAGACTCAGACACAAGGC+AGG 0.587110 1_4:+58586521 None:intergenic
CCTGGAGAACGGTGTCGCGC+CGG 0.589874 1_4:+58586462 None:intergenic
TTGACGAAGTGGACGCAGAA+GGG 0.593699 1_4:+58585966 None:intergenic
CCATATTTGTAGTAGAGTTG+AGG 0.596000 1_4:-58586181 Msa0173350:five_prime_UTR
GTTGACGAAGTGGACGCAGA+AGG 0.597332 1_4:+58585965 None:intergenic
TTGTCGAAGCAAAACGGAAG+GGG 0.600576 1_4:+58586499 None:intergenic
TTTGTAGTAGAGTTGAGGCG+TGG 0.611994 1_4:-58586176 Msa0173350:five_prime_UTR
ACGGAGGCAGTCCTGGAGAA+CGG 0.612455 1_4:+58586451 None:intergenic
GGCGGAGGCTGCAGTGACGG+AGG 0.613435 1_4:+58585624 None:intergenic
GAATATAGTAGGAGATACCA+TGG 0.619077 1_4:-58585667 Msa0173350:CDS
CTGCGTCCACTTCGTCAACA+CGG 0.619782 1_4:-58585961 Msa0173350:intron
TTGCATACAGGGAGGCCCAA+AGG 0.621250 1_4:+58585544 None:intergenic
AGCGTCGTCGCGCAGAACGG+AGG 0.626515 1_4:+58586435 None:intergenic
GGTTGTCGAAGCAAAACGGA+AGG 0.626766 1_4:+58586497 None:intergenic
TCGCGCCGGACCTAGAACAG+AGG 0.642209 1_4:+58586476 None:intergenic
AAAGATCATAATTCATAACA+AGG 0.646758 1_4:+58585405 None:intergenic
ACAAACAAGCCAAAGCTCGT+TGG 0.650557 1_4:-58586043 Msa0173350:CDS
ATTGCCGAACGACACTCCCA+TGG 0.650589 1_4:+58585650 None:intergenic
GTTGTCGAAGCAAAACGGAA+GGG 0.655524 1_4:+58586498 None:intergenic
CAGAAGGGTTGATTAGATGG+TGG 0.659317 1_4:+58585981 None:intergenic
TTGGAGTATCCACCCTCAGG+CGG 0.670531 1_4:+58585606 None:intergenic
ATCCCCAGTCGACAAATGGG+AGG 0.671698 1_4:+58585583 None:intergenic
AACCTCCCATTTGTCGACTG+GGG 0.672189 1_4:-58585585 Msa0173350:CDS
AGGGGTCAGACTCAGACACA+AGG 0.684771 1_4:+58586517 None:intergenic
AATATAGTAGGAGATACCAT+GGG 0.697591 1_4:-58585666 Msa0173350:CDS
TGTGTTGAATACCGGCAGAG+TGG 0.711189 1_4:+58586063 None:intergenic
AATAATGGTTGCATACAGGG+AGG 0.744657 1_4:+58585536 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGAATTAAAACTCAATTAA+TGG + chr1_4:58585683-58585702 None:intergenic 15.0%
!! ATTTATAGTTTCTTTCTTTA+TGG - chr1_4:58586190-58586209 Msa0173350:five_prime_UTR 15.0%
!! TTGAATTAAAACTCAATTAA+TGG + chr1_4:58585683-58585702 None:intergenic 15.0%
!! ATTTATAGTTTCTTTCTTTA+TGG - chr1_4:58586190-58586209 Msa0173350:five_prime_UTR 15.0%
!! CTAAGAAATGATAATTAATG+AGG + chr1_4:58586023-58586042 None:intergenic 20.0%
!! TCATTATAGCCATAAAATAA+TGG + chr1_4:58586412-58586431 None:intergenic 20.0%
!! AAAGATCATAATTCATAACA+AGG + chr1_4:58586528-58586547 None:intergenic 20.0%
!!! TCTTTTTTAGATATGTATAG+AGG + chr1_4:58586559-58586578 None:intergenic 20.0%
!! CTAAGAAATGATAATTAATG+AGG + chr1_4:58586023-58586042 None:intergenic 20.0%
!! TCATTATAGCCATAAAATAA+TGG + chr1_4:58586412-58586431 None:intergenic 20.0%
!! AAAGATCATAATTCATAACA+AGG + chr1_4:58586528-58586547 None:intergenic 20.0%
!!! TCTTTTTTAGATATGTATAG+AGG + chr1_4:58586559-58586578 None:intergenic 20.0%
! AACTTTCGATCATAAAACAA+AGG + chr1_4:58585368-58585387 None:intergenic 25.0%
! TCATACAAGTCATTTGTAAT+TGG - chr1_4:58585552-58585571 Msa0173350:three_prime_UTR 25.0%
!!! TCACAATAGTACTTTTTCAT+TGG + chr1_4:58585802-58585821 None:intergenic 25.0%
! ATTAACTCTCTCTGTATATT+AGG - chr1_4:58586055-58586074 Msa0173350:CDS 25.0%
! TTAACTCTCTCTGTATATTA+GGG - chr1_4:58586056-58586075 Msa0173350:CDS 25.0%
! ATCAGTAAATGAACAACTAT+CGG + chr1_4:58586172-58586191 None:intergenic 25.0%
! TATGTACTATGCATATATGT+AGG - chr1_4:58586240-58586259 Msa0173350:intron 25.0%
! ATGTACTATGCATATATGTA+GGG - chr1_4:58586241-58586260 Msa0173350:intron 25.0%
! ATATATGTAGGGAATATAGT+AGG - chr1_4:58586252-58586271 Msa0173350:intron 25.0%
! TAAAATAATGGTTGCATACA+GGG + chr1_4:58586400-58586419 None:intergenic 25.0%
! ATAAAATAATGGTTGCATAC+AGG + chr1_4:58586401-58586420 None:intergenic 25.0%
!!! TGTATGCAACCATTATTTTA+TGG - chr1_4:58586400-58586419 Msa0173350:intron 25.0%
!!! AACATACTAATAGTTCTGAT+AGG - chr1_4:58586432-58586451 Msa0173350:five_prime_UTR 25.0%
! AACTTTCGATCATAAAACAA+AGG + chr1_4:58585368-58585387 None:intergenic 25.0%
! TCATACAAGTCATTTGTAAT+TGG - chr1_4:58585552-58585571 Msa0173350:three_prime_UTR 25.0%
!!! TCACAATAGTACTTTTTCAT+TGG + chr1_4:58585802-58585821 None:intergenic 25.0%
! ATTAACTCTCTCTGTATATT+AGG - chr1_4:58586055-58586074 Msa0173350:CDS 25.0%
! TTAACTCTCTCTGTATATTA+GGG - chr1_4:58586056-58586075 Msa0173350:CDS 25.0%
! ATCAGTAAATGAACAACTAT+CGG + chr1_4:58586172-58586191 None:intergenic 25.0%
! TATGTACTATGCATATATGT+AGG - chr1_4:58586240-58586259 Msa0173350:intron 25.0%
! ATGTACTATGCATATATGTA+GGG - chr1_4:58586241-58586260 Msa0173350:intron 25.0%
! ATATATGTAGGGAATATAGT+AGG - chr1_4:58586252-58586271 Msa0173350:intron 25.0%
! TAAAATAATGGTTGCATACA+GGG + chr1_4:58586400-58586419 None:intergenic 25.0%
! ATAAAATAATGGTTGCATAC+AGG + chr1_4:58586401-58586420 None:intergenic 25.0%
!!! TGTATGCAACCATTATTTTA+TGG - chr1_4:58586400-58586419 Msa0173350:intron 25.0%
!!! AACATACTAATAGTTCTGAT+AGG - chr1_4:58586432-58586451 Msa0173350:five_prime_UTR 25.0%
TACAAGTCATTTGTAATTGG+CGG - chr1_4:58585555-58585574 Msa0173350:three_prime_UTR 30.0%
ATGAAACAAGAGCAAAGATA+AGG + chr1_4:58585626-58585645 None:intergenic 30.0%
! TATTAGGGATTTTGTCGTTA+TGG - chr1_4:58586071-58586090 Msa0173350:CDS 30.0%
TCAGTAAATGAACAACTATC+GGG + chr1_4:58586171-58586190 None:intergenic 30.0%
AATATAGTAGGAGATACCAT+GGG - chr1_4:58586264-58586283 Msa0173350:intron 30.0%
!!! CTGATTTTGAGTTTCTTTAC+TGG - chr1_4:58586461-58586480 Msa0173350:five_prime_UTR 30.0%
! TACTGGTTATTACAACTACT+AGG - chr1_4:58586478-58586497 Msa0173350:five_prime_UTR 30.0%
TACAAGTCATTTGTAATTGG+CGG - chr1_4:58585555-58585574 Msa0173350:three_prime_UTR 30.0%
ATGAAACAAGAGCAAAGATA+AGG + chr1_4:58585626-58585645 None:intergenic 30.0%
! TATTAGGGATTTTGTCGTTA+TGG - chr1_4:58586071-58586090 Msa0173350:CDS 30.0%
TCAGTAAATGAACAACTATC+GGG + chr1_4:58586171-58586190 None:intergenic 30.0%
AATATAGTAGGAGATACCAT+GGG - chr1_4:58586264-58586283 Msa0173350:intron 30.0%
!!! CTGATTTTGAGTTTCTTTAC+TGG - chr1_4:58586461-58586480 Msa0173350:five_prime_UTR 30.0%
! TACTGGTTATTACAACTACT+AGG - chr1_4:58586478-58586497 Msa0173350:five_prime_UTR 30.0%
AGCAAAGATAAGGATTGTTC+GGG + chr1_4:58585616-58585635 None:intergenic 35.0%
GAGCAAAGATAAGGATTGTT+CGG + chr1_4:58585617-58585636 None:intergenic 35.0%
! CTCTTGTTTCATGTTCACAT+GGG - chr1_4:58585634-58585653 Msa0173350:CDS 35.0%
CCTCAACTCTACTACAAATA+TGG + chr1_4:58585752-58585771 None:intergenic 35.0%
CCATATTTGTAGTAGAGTTG+AGG - chr1_4:58585749-58585768 Msa0173350:intron 35.0%
CAGTAAATGAACAACTATCG+GGG + chr1_4:58586170-58586189 None:intergenic 35.0%
GAATATAGTAGGAGATACCA+TGG - chr1_4:58586263-58586282 Msa0173350:intron 35.0%
AGATTTGTGCACATTACCTT+TGG - chr1_4:58586370-58586389 Msa0173350:intron 35.0%
GATTTGTGCACATTACCTTT+GGG - chr1_4:58586371-58586390 Msa0173350:intron 35.0%
AGATATGTATAGAGGAGAGA+TGG + chr1_4:58586551-58586570 None:intergenic 35.0%
AGCAAAGATAAGGATTGTTC+GGG + chr1_4:58585616-58585635 None:intergenic 35.0%
GAGCAAAGATAAGGATTGTT+CGG + chr1_4:58585617-58585636 None:intergenic 35.0%
! CTCTTGTTTCATGTTCACAT+GGG - chr1_4:58585634-58585653 Msa0173350:CDS 35.0%
CCTCAACTCTACTACAAATA+TGG + chr1_4:58585752-58585771 None:intergenic 35.0%
CCATATTTGTAGTAGAGTTG+AGG - chr1_4:58585749-58585768 Msa0173350:intron 35.0%
CAGTAAATGAACAACTATCG+GGG + chr1_4:58586170-58586189 None:intergenic 35.0%
GAATATAGTAGGAGATACCA+TGG - chr1_4:58586263-58586282 Msa0173350:intron 35.0%
AGATTTGTGCACATTACCTT+TGG - chr1_4:58586370-58586389 Msa0173350:intron 35.0%
GATTTGTGCACATTACCTTT+GGG - chr1_4:58586371-58586390 Msa0173350:intron 35.0%
AGATATGTATAGAGGAGAGA+TGG + chr1_4:58586551-58586570 None:intergenic 35.0%
ATGTGAGCGTAGAAAGGAAT+TGG + chr1_4:58585524-58585543 None:intergenic 40.0%
TCCTTTCTACGCTCACATTA+AGG - chr1_4:58585526-58585545 Msa0173350:three_prime_UTR 40.0%
ACCTTAATGTGAGCGTAGAA+AGG + chr1_4:58585530-58585549 None:intergenic 40.0%
! GCTCTTGTTTCATGTTCACA+TGG - chr1_4:58585633-58585652 Msa0173350:CDS 40.0%
TTCAAGATACACCCTTAGAC+CGG - chr1_4:58585839-58585858 Msa0173350:CDS 40.0%
! GGCTTGTTTGTGTTGAATAC+CGG + chr1_4:58585878-58585897 None:intergenic 40.0%
! TGGAATAGAGTTCTGGTGTT+CGG + chr1_4:58585929-58585948 None:intergenic 40.0%
CAACCAATAACAAGAATGCC+AGG - chr1_4:58586137-58586156 Msa0173350:CDS 40.0%
! TCACCTGGCATTCTTGTTAT+TGG + chr1_4:58586143-58586162 None:intergenic 40.0%
AATAATGGTTGCATACAGGG+AGG + chr1_4:58586397-58586416 None:intergenic 40.0%
ATGTGAGCGTAGAAAGGAAT+TGG + chr1_4:58585524-58585543 None:intergenic 40.0%
TCCTTTCTACGCTCACATTA+AGG - chr1_4:58585526-58585545 Msa0173350:three_prime_UTR 40.0%
ACCTTAATGTGAGCGTAGAA+AGG + chr1_4:58585530-58585549 None:intergenic 40.0%
! GCTCTTGTTTCATGTTCACA+TGG - chr1_4:58585633-58585652 Msa0173350:CDS 40.0%
TTCAAGATACACCCTTAGAC+CGG - chr1_4:58585839-58585858 Msa0173350:CDS 40.0%
! GGCTTGTTTGTGTTGAATAC+CGG + chr1_4:58585878-58585897 None:intergenic 40.0%
! TGGAATAGAGTTCTGGTGTT+CGG + chr1_4:58585929-58585948 None:intergenic 40.0%
CAACCAATAACAAGAATGCC+AGG - chr1_4:58586137-58586156 Msa0173350:CDS 40.0%
! TCACCTGGCATTCTTGTTAT+TGG + chr1_4:58586143-58586162 None:intergenic 40.0%
AATAATGGTTGCATACAGGG+AGG + chr1_4:58586397-58586416 None:intergenic 40.0%
GGCAGGCATACAAGGAAAAT+GGG + chr1_4:58585395-58585414 None:intergenic 45.0%
AGGCAGGCATACAAGGAAAA+TGG + chr1_4:58585396-58585415 None:intergenic 45.0%
TTGTCGAAGCAAAACGGAAG+GGG + chr1_4:58585434-58585453 None:intergenic 45.0%
GTTGTCGAAGCAAAACGGAA+GGG + chr1_4:58585435-58585454 None:intergenic 45.0%
CAGAGGTTGTCGAAGCAAAA+CGG + chr1_4:58585440-58585459 None:intergenic 45.0%
TTGCAACTTCAAGCTTCGTG+CGG + chr1_4:58585586-58585605 None:intergenic 45.0%
TTTGTAGTAGAGTTGAGGCG+TGG - chr1_4:58585754-58585773 Msa0173350:intron 45.0%
TTGTAGTAGAGTTGAGGCGT+GGG - chr1_4:58585755-58585774 Msa0173350:intron 45.0%
ACAAACAAGCCAAAGCTCGT+TGG - chr1_4:58585887-58585906 Msa0173350:intron 45.0%
! GGAATAGAGTTCTGGTGTTC+GGG + chr1_4:58585928-58585947 None:intergenic 45.0%
!! ATGGTGGTGGAATAGAGTTC+TGG + chr1_4:58585936-58585955 None:intergenic 45.0%
! AAGGGTTGATTAGATGGTGG+TGG + chr1_4:58585949-58585968 None:intergenic 45.0%
! CAGAAGGGTTGATTAGATGG+TGG + chr1_4:58585952-58585971 None:intergenic 45.0%
! ACGCAGAAGGGTTGATTAGA+TGG + chr1_4:58585955-58585974 None:intergenic 45.0%
! ATAGTACCGTGTTGACGAAG+TGG + chr1_4:58585978-58585997 None:intergenic 45.0%
TGCTGCTGCTGTTGTGTATT+GGG + chr1_4:58586119-58586138 None:intergenic 45.0%
TTGCTGCTGCTGTTGTGTAT+TGG + chr1_4:58586120-58586139 None:intergenic 45.0%
TCTATCCCCAGTCGACAAAT+GGG + chr1_4:58586353-58586372 None:intergenic 45.0%
ATCTATCCCCAGTCGACAAA+TGG + chr1_4:58586354-58586373 None:intergenic 45.0%
GGCAGGCATACAAGGAAAAT+GGG + chr1_4:58585395-58585414 None:intergenic 45.0%
AGGCAGGCATACAAGGAAAA+TGG + chr1_4:58585396-58585415 None:intergenic 45.0%
TTGTCGAAGCAAAACGGAAG+GGG + chr1_4:58585434-58585453 None:intergenic 45.0%
GTTGTCGAAGCAAAACGGAA+GGG + chr1_4:58585435-58585454 None:intergenic 45.0%
CAGAGGTTGTCGAAGCAAAA+CGG + chr1_4:58585440-58585459 None:intergenic 45.0%
TTGCAACTTCAAGCTTCGTG+CGG + chr1_4:58585586-58585605 None:intergenic 45.0%
TTTGTAGTAGAGTTGAGGCG+TGG - chr1_4:58585754-58585773 Msa0173350:intron 45.0%
TTGTAGTAGAGTTGAGGCGT+GGG - chr1_4:58585755-58585774 Msa0173350:intron 45.0%
ACAAACAAGCCAAAGCTCGT+TGG - chr1_4:58585887-58585906 Msa0173350:intron 45.0%
! GGAATAGAGTTCTGGTGTTC+GGG + chr1_4:58585928-58585947 None:intergenic 45.0%
!! ATGGTGGTGGAATAGAGTTC+TGG + chr1_4:58585936-58585955 None:intergenic 45.0%
! AAGGGTTGATTAGATGGTGG+TGG + chr1_4:58585949-58585968 None:intergenic 45.0%
! CAGAAGGGTTGATTAGATGG+TGG + chr1_4:58585952-58585971 None:intergenic 45.0%
! ACGCAGAAGGGTTGATTAGA+TGG + chr1_4:58585955-58585974 None:intergenic 45.0%
! ATAGTACCGTGTTGACGAAG+TGG + chr1_4:58585978-58585997 None:intergenic 45.0%
TGCTGCTGCTGTTGTGTATT+GGG + chr1_4:58586119-58586138 None:intergenic 45.0%
TTGCTGCTGCTGTTGTGTAT+TGG + chr1_4:58586120-58586139 None:intergenic 45.0%
TCTATCCCCAGTCGACAAAT+GGG + chr1_4:58586353-58586372 None:intergenic 45.0%
ATCTATCCCCAGTCGACAAA+TGG + chr1_4:58586354-58586373 None:intergenic 45.0%
AGACACAAGGCAGGCATACA+AGG + chr1_4:58585403-58585422 None:intergenic 50.0%
GGTTGTCGAAGCAAAACGGA+AGG + chr1_4:58585436-58585455 None:intergenic 50.0%
GCTTCGACAACCTCTGTTCT+AGG - chr1_4:58585444-58585463 Msa0173350:three_prime_UTR 50.0%
! GACAACCTCTGTTCTAGGTC+CGG - chr1_4:58585449-58585468 Msa0173350:three_prime_UTR 50.0%
TGTGTTGAATACCGGCAGAG+TGG + chr1_4:58585870-58585889 None:intergenic 50.0%
GGAATCGTACCAACGAGCTT+TGG + chr1_4:58585899-58585918 None:intergenic 50.0%
! GTTCTGGTGTTCGGGTTTGA+AGG + chr1_4:58585920-58585939 None:intergenic 50.0%
TTGACGAAGTGGACGCAGAA+GGG + chr1_4:58585967-58585986 None:intergenic 50.0%
!! GATACCATGGGAGTGTCGTT+CGG - chr1_4:58586276-58586295 Msa0173350:intron 50.0%
! CAACCTCCCATTTGTCGACT+GGG - chr1_4:58586344-58586363 Msa0173350:intron 50.0%
! AACCTCCCATTTGTCGACTG+GGG - chr1_4:58586345-58586364 Msa0173350:intron 50.0%
AGACACAAGGCAGGCATACA+AGG + chr1_4:58585403-58585422 None:intergenic 50.0%
GGTTGTCGAAGCAAAACGGA+AGG + chr1_4:58585436-58585455 None:intergenic 50.0%
GCTTCGACAACCTCTGTTCT+AGG - chr1_4:58585444-58585463 Msa0173350:three_prime_UTR 50.0%
! GACAACCTCTGTTCTAGGTC+CGG - chr1_4:58585449-58585468 Msa0173350:three_prime_UTR 50.0%
TGTGTTGAATACCGGCAGAG+TGG + chr1_4:58585870-58585889 None:intergenic 50.0%
GGAATCGTACCAACGAGCTT+TGG + chr1_4:58585899-58585918 None:intergenic 50.0%
! GTTCTGGTGTTCGGGTTTGA+AGG + chr1_4:58585920-58585939 None:intergenic 50.0%
TTGACGAAGTGGACGCAGAA+GGG + chr1_4:58585967-58585986 None:intergenic 50.0%
!! GATACCATGGGAGTGTCGTT+CGG - chr1_4:58586276-58586295 Msa0173350:intron 50.0%
! CAACCTCCCATTTGTCGACT+GGG - chr1_4:58586344-58586363 Msa0173350:intron 50.0%
! AACCTCCCATTTGTCGACTG+GGG - chr1_4:58586345-58586364 Msa0173350:intron 50.0%
GTCAGACTCAGACACAAGGC+AGG + chr1_4:58585412-58585431 None:intergenic 55.0%
AGGGGTCAGACTCAGACACA+AGG + chr1_4:58585416-58585435 None:intergenic 55.0%
CAACTTCAAGCTTCGTGCGG+TGG + chr1_4:58585583-58585602 None:intergenic 55.0%
GAGTGGCGTTTCCGGTCTAA+GGG + chr1_4:58585853-58585872 None:intergenic 55.0%
AGAGTGGCGTTTCCGGTCTA+AGG + chr1_4:58585854-58585873 None:intergenic 55.0%
GTTGACGAAGTGGACGCAGA+AGG + chr1_4:58585968-58585987 None:intergenic 55.0%
CTGCGTCCACTTCGTCAACA+CGG - chr1_4:58585969-58585988 Msa0173350:CDS 55.0%
AACTATCGGGGTAGCTCACC+TGG + chr1_4:58586158-58586177 None:intergenic 55.0%
ATTGCCGAACGACACTCCCA+TGG + chr1_4:58586283-58586302 None:intergenic 55.0%
! TTGGAGTATCCACCCTCAGG+CGG + chr1_4:58586327-58586346 None:intergenic 55.0%
! AGGTTGGAGTATCCACCCTC+AGG + chr1_4:58586330-58586349 None:intergenic 55.0%
CCAGTCGACAAATGGGAGGT+TGG + chr1_4:58586346-58586365 None:intergenic 55.0%
CCAACCTCCCATTTGTCGAC+TGG - chr1_4:58586343-58586362 Msa0173350:intron 55.0%
ATCCCCAGTCGACAAATGGG+AGG + chr1_4:58586350-58586369 None:intergenic 55.0%
TTGCATACAGGGAGGCCCAA+AGG + chr1_4:58586389-58586408 None:intergenic 55.0%
GTCAGACTCAGACACAAGGC+AGG + chr1_4:58585412-58585431 None:intergenic 55.0%
AGGGGTCAGACTCAGACACA+AGG + chr1_4:58585416-58585435 None:intergenic 55.0%
CAACTTCAAGCTTCGTGCGG+TGG + chr1_4:58585583-58585602 None:intergenic 55.0%
GAGTGGCGTTTCCGGTCTAA+GGG + chr1_4:58585853-58585872 None:intergenic 55.0%
AGAGTGGCGTTTCCGGTCTA+AGG + chr1_4:58585854-58585873 None:intergenic 55.0%
GTTGACGAAGTGGACGCAGA+AGG + chr1_4:58585968-58585987 None:intergenic 55.0%
CTGCGTCCACTTCGTCAACA+CGG - chr1_4:58585969-58585988 Msa0173350:CDS 55.0%
AACTATCGGGGTAGCTCACC+TGG + chr1_4:58586158-58586177 None:intergenic 55.0%
ATTGCCGAACGACACTCCCA+TGG + chr1_4:58586283-58586302 None:intergenic 55.0%
! TTGGAGTATCCACCCTCAGG+CGG + chr1_4:58586327-58586346 None:intergenic 55.0%
! AGGTTGGAGTATCCACCCTC+AGG + chr1_4:58586330-58586349 None:intergenic 55.0%
CCAGTCGACAAATGGGAGGT+TGG + chr1_4:58586346-58586365 None:intergenic 55.0%
CCAACCTCCCATTTGTCGAC+TGG - chr1_4:58586343-58586362 Msa0173350:intron 55.0%
ATCCCCAGTCGACAAATGGG+AGG + chr1_4:58586350-58586369 None:intergenic 55.0%
TTGCATACAGGGAGGCCCAA+AGG + chr1_4:58586389-58586408 None:intergenic 55.0%
ACGGAGGCAGTCCTGGAGAA+CGG + chr1_4:58585482-58585501 None:intergenic 60.0%
TACAGCGTCGTCGCGCAGAA+CGG + chr1_4:58585501-58585520 None:intergenic 60.0%
GTAGAGTTGAGGCGTGGGTG+TGG - chr1_4:58585760-58585779 Msa0173350:intron 60.0%
TACCGGCAGAGTGGCGTTTC+CGG + chr1_4:58585861-58585880 None:intergenic 60.0%
ACGGAGGCAGTCCTGGAGAA+CGG + chr1_4:58585482-58585501 None:intergenic 60.0%
TACAGCGTCGTCGCGCAGAA+CGG + chr1_4:58585501-58585520 None:intergenic 60.0%
GTAGAGTTGAGGCGTGGGTG+TGG - chr1_4:58585760-58585779 Msa0173350:intron 60.0%
TACCGGCAGAGTGGCGTTTC+CGG + chr1_4:58585861-58585880 None:intergenic 60.0%
TCGCGCCGGACCTAGAACAG+AGG + chr1_4:58585457-58585476 None:intergenic 65.0%
GTTGAGGCGTGGGTGTGGTG+AGG - chr1_4:58585765-58585784 Msa0173350:intron 65.0%
AGGCGTGGGTGTGGTGAGGA+GGG - chr1_4:58585769-58585788 Msa0173350:intron 65.0%
GACCGGAAACGCCACTCTGC+CGG - chr1_4:58585856-58585875 Msa0173350:CDS 65.0%
TCAGGCGGAGGCTGCAGTGA+CGG + chr1_4:58586312-58586331 None:intergenic 65.0%
TCACTGCAGCCTCCGCCTGA+GGG - chr1_4:58586312-58586331 Msa0173350:intron 65.0%
GAGTATCCACCCTCAGGCGG+AGG + chr1_4:58586324-58586343 None:intergenic 65.0%
TCGCGCCGGACCTAGAACAG+AGG + chr1_4:58585457-58585476 None:intergenic 65.0%
GTTGAGGCGTGGGTGTGGTG+AGG - chr1_4:58585765-58585784 Msa0173350:intron 65.0%
AGGCGTGGGTGTGGTGAGGA+GGG - chr1_4:58585769-58585788 Msa0173350:intron 65.0%
GACCGGAAACGCCACTCTGC+CGG - chr1_4:58585856-58585875 Msa0173350:CDS 65.0%
TCAGGCGGAGGCTGCAGTGA+CGG + chr1_4:58586312-58586331 None:intergenic 65.0%
TCACTGCAGCCTCCGCCTGA+GGG - chr1_4:58586312-58586331 Msa0173350:intron 65.0%
GAGTATCCACCCTCAGGCGG+AGG + chr1_4:58586324-58586343 None:intergenic 65.0%
!! CCTGGAGAACGGTGTCGCGC+CGG + chr1_4:58585471-58585490 None:intergenic 70.0%
GCGCAGAACGGAGGCAGTCC+TGG + chr1_4:58585489-58585508 None:intergenic 70.0%
AGCGTCGTCGCGCAGAACGG+AGG + chr1_4:58585498-58585517 None:intergenic 70.0%
GAGGCGTGGGTGTGGTGAGG+AGG - chr1_4:58585768-58585787 Msa0173350:intron 70.0%
GGCGTGGGTGTGGTGAGGAG+GGG - chr1_4:58585770-58585789 Msa0173350:intron 70.0%
GTCACTGCAGCCTCCGCCTG+AGG - chr1_4:58586311-58586330 Msa0173350:intron 70.0%
!! CCTGGAGAACGGTGTCGCGC+CGG + chr1_4:58585471-58585490 None:intergenic 70.0%
GCGCAGAACGGAGGCAGTCC+TGG + chr1_4:58585489-58585508 None:intergenic 70.0%
AGCGTCGTCGCGCAGAACGG+AGG + chr1_4:58585498-58585517 None:intergenic 70.0%
GAGGCGTGGGTGTGGTGAGG+AGG - chr1_4:58585768-58585787 Msa0173350:intron 70.0%
GGCGTGGGTGTGGTGAGGAG+GGG - chr1_4:58585770-58585789 Msa0173350:intron 70.0%
GTCACTGCAGCCTCCGCCTG+AGG - chr1_4:58586311-58586330 Msa0173350:intron 70.0%
CCGGCGCGACACCGTTCTCC+AGG - chr1_4:58585468-58585487 Msa0173350:three_prime_UTR 75.0%
GGCGGAGGCTGCAGTGACGG+AGG + chr1_4:58586309-58586328 None:intergenic 75.0%
CTGCAGCCTCCGCCTGAGGG+TGG - chr1_4:58586315-58586334 Msa0173350:intron 75.0%
CCGGCGCGACACCGTTCTCC+AGG - chr1_4:58585468-58585487 Msa0173350:three_prime_UTR 75.0%
GGCGGAGGCTGCAGTGACGG+AGG + chr1_4:58586309-58586328 None:intergenic 75.0%
CTGCAGCCTCCGCCTGAGGG+TGG - chr1_4:58586315-58586334 Msa0173350:intron 75.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 58585361 58586591 58585361 ID=Msa0173350;Name=Msa0173350
chr1_4 mRNA 58585361 58586591 58585361 ID=Msa0173350-mRNA-1;Parent=Msa0173350;Name=Msa0173350-mRNA-1;_AED=0.01;_eAED=0.01;_QI=251|1|1|1|0.66|0.75|4|220|128
chr1_4 exon 58585361 58585690 58585361 ID=Msa0173350-mRNA-1:exon:18214;Parent=Msa0173350-mRNA-1
chr1_4 exon 58585794 58585875 58585794 ID=Msa0173350-mRNA-1:exon:18213;Parent=Msa0173350-mRNA-1
chr1_4 exon 58585962 58586220 58585962 ID=Msa0173350-mRNA-1:exon:18212;Parent=Msa0173350-mRNA-1
chr1_4 exon 58586405 58586591 58586405 ID=Msa0173350-mRNA-1:exon:18211;Parent=Msa0173350-mRNA-1
chr1_4 five_prime_UTR 58586405 58586591 58586405 ID=Msa0173350-mRNA-1:five_prime_utr;Parent=Msa0173350-mRNA-1
chr1_4 five_prime_UTR 58586157 58586220 58586157 ID=Msa0173350-mRNA-1:five_prime_utr;Parent=Msa0173350-mRNA-1
chr1_4 CDS 58585962 58586156 58585962 ID=Msa0173350-mRNA-1:cds;Parent=Msa0173350-mRNA-1
chr1_4 CDS 58585794 58585875 58585794 ID=Msa0173350-mRNA-1:cds;Parent=Msa0173350-mRNA-1
chr1_4 CDS 58585581 58585690 58585581 ID=Msa0173350-mRNA-1:cds;Parent=Msa0173350-mRNA-1
chr1_4 three_prime_UTR 58585361 58585580 58585361 ID=Msa0173350-mRNA-1:three_prime_utr;Parent=Msa0173350-mRNA-1
Gene Sequence

>Msa0173350

ATGCCAATGAAAAAGTACTATTGTGAGTATTGCGATAAGCAATTTCAAGATACACCCTTAGACCGGAAACGCCACTCTGCCGGTATTCAACACAAACAAGCCAAAGCTCGTTGGTACGATTCCTTCAAACCCGAACACCAGAACTCTATTCCACCACCATCTAATCAACCCTTCTGCGTCCACTTCGTCAACACGGGATTTTGTCGTTATGGCGATTCTTGCAAATATTTTCATCCCAATACACAACAGCAGCAGCAACCAATAACAAGAATGCCAGGGAATATAGTAGGAGATACCATGGGAGTGTCGTTCGGCAATTTGCCTCCGTCACTGCAGCCTCCGCCTGAGGGTGGATACTCCAACCTCCCATTTGTCGACTGGGGATAG
Protein sequence

>Msa0173350

MPMKKYYCEYCDKQFQDTPLDRKRHSAGIQHKQAKARWYDSFKPEHQNSIPPPSNQPFCVHFVNTGFCRYGDSCKYFHPNTQQQQQPITRMPGNIVGDTMGVSFGNLPPSLQPPPEGGYSNLPFVDWG*