AlfalfaGEDB | Msa0174020

M. sativa cultivar zhongmu-4 / Msa0174020

KEGG: K09422
GO: -
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0174020	AHY94990.1	97.482	278	7	0	1	278	1	278	0.0	562

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0174020	sp\|Q10MB4\|MYB2_ORYSJ	73.944	142	35	1	26	165	11	152	5.28e-76	237

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0174020	A0A024A419	97.482	278	7	0	1	278	1	278	0.0	562

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TFs/TRs:

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Gene ID	Type	Classification
Msa0174020	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0174020	MtrunA17_Chr1g0194301	95.324	278	13	0	1	278	1	278	0.0	549
Msa0174020	MtrunA17_Chr1g0194291	56.641	256	93	5	26	276	14	256	6.97e-91	270
Msa0174020	MtrunA17_Chr4g0068921	74.468	141	36	0	26	166	17	157	2.78e-72	220
Msa0174020	MtrunA17_Chr2g0293911	70.748	147	43	0	20	166	11	157	3.30e-70	219
Msa0174020	MtrunA17_Chr1g0209661	56.287	167	70	1	33	199	27	190	1.40e-67	212
Msa0174020	MtrunA17_Chr7g0228231	68.966	116	36	0	33	148	14	129	1.27e-57	183
Msa0174020	MtrunA17_Chr6g0450101	72.222	108	30	0	33	140	14	121	4.40e-57	182
Msa0174020	MtrunA17_Chr1g0168661	69.159	107	33	0	32	138	20	126	1.39e-51	169
Msa0174020	MtrunA17_Chr1g0186061	60.833	120	45	1	19	138	8	125	3.48e-49	163
Msa0174020	MtrunA17_Chr2g0327821	59.633	109	44	0	33	141	6	114	8.84e-46	154

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0174020	AT2G47190.1	48.679	265	112	5	26	277	13	266	1.35e-80	244
Msa0174020	AT3G06490.1	74.324	148	38	0	19	166	5	152	3.18e-75	233
Msa0174020	AT1G48000.1	67.347	147	47	1	33	178	32	178	6.34e-73	224
Msa0174020	AT5G49620.1	74.468	141	36	0	28	168	21	161	1.75e-71	222
Msa0174020	AT1G68320.1	66.901	142	47	0	33	174	19	160	9.10e-70	217
Msa0174020	AT5G49620.2	67.742	155	36	1	28	168	21	175	7.97e-68	213
Msa0174020	AT1G25340.1	52.020	198	64	4	34	231	19	185	7.41e-65	204
Msa0174020	AT1G25340.2	50.000	198	63	5	34	231	19	180	2.74e-59	190
Msa0174020	AT1G25340.3	50.000	198	63	5	34	231	19	180	4.76e-59	191
Msa0174020	AT3G27810.1	72.897	107	29	0	33	139	20	126	1.59e-57	184

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Find 66 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATAGCTGAACAATTACC+GGG	0.587045	1_4:+59533462	Msa0174020:CDS
AAACCAATATTCATGTAATG+AGG	0.554808	1_4:+59532844	Msa0174020:CDS
AAACGTATCTTACAATTTAA+TGG	0.137838	1_4:+59533791	Msa0174020:CDS
AAAGCTCATAGTGAAACTTG+TGG	0.646540	1_4:+59533693	Msa0174020:CDS
AACTCCGTCGCTCGCTGTTC+GGG	0.308012	1_4:+59533002	Msa0174020:CDS
AAGCTCATAGTGAAACTTGT+GGG	0.537240	1_4:+59533694	Msa0174020:CDS
ACAATGAAATAAAGAACTAT+TGG	0.404212	1_4:+59533493	Msa0174020:CDS
ACACGTACCGATTGCCCCAA+CGG	0.518769	1_4:-59533139	None:intergenic
ACCTCTGACTGCAACATGGG+AGG	0.606494	1_4:-59533729	None:intergenic
ACTCCTCATTACATGAATAT+TGG	0.255386	1_4:-59532847	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACTCCTCATTACATGAATAT+TGG	-	chr1_4:59532850-59532869	None:intergenic	30.0%
TGTAAGTCAAGAAGATACTA+TGG	+	chr1_4:59532899-59532918	Msa0174020:CDS	30.0%
GATACTATGGTGATAAAGAA+AGG	+	chr1_4:59532912-59532931	Msa0174020:CDS	30.0%
TACTATTCTGATGAACTATG+TGG	+	chr1_4:59532956-59532975	Msa0174020:CDS	30.0%
AAAATAGCTGAACAATTACC+GGG	+	chr1_4:59533462-59533481	Msa0174020:CDS	30.0%
ATGTAAACAGTCAACAGTTT+AGG	+	chr1_4:59533556-59533575	Msa0174020:CDS	30.0%
TGTAAACAGTCAACAGTTTA+GGG	+	chr1_4:59533557-59533576	Msa0174020:CDS	30.0%
TGTGGACAGATGAAAATATA+TGG	+	chr1_4:59533910-59533929	Msa0174020:CDS	30.0%
ACTCCTCATTACATGAATAT+TGG	-	chr1_4:59532850-59532869	None:intergenic	30.0%
TGTAAGTCAAGAAGATACTA+TGG	+	chr1_4:59532899-59532918	Msa0174020:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	59532828	59533965	59532828	ID=Msa0174020;Name=Msa0174020
chr1_4	mRNA	59532828	59533965	59532828	ID=Msa0174020-mRNA-1;Parent=Msa0174020;Name=Msa0174020-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0\|1\|0\|1\|1\|1\|2\|0\|278
chr1_4	exon	59532828	59533153	59532828	ID=Msa0174020-mRNA-1:exon:18688;Parent=Msa0174020-mRNA-1
chr1_4	exon	59533455	59533965	59533455	ID=Msa0174020-mRNA-1:exon:18689;Parent=Msa0174020-mRNA-1
chr1_4	CDS	59532828	59533153	59532828	ID=Msa0174020-mRNA-1:cds;Parent=Msa0174020-mRNA-1
chr1_4	CDS	59533455	59533965	59533455	ID=Msa0174020-mRNA-1:cds;Parent=Msa0174020-mRNA-1

Gene Sequence

>Msa0174020

ATGGATACTAATTACAAAACCAATATTCATGTAATGAGGAGTTTTTCAGATTGTGGTTCATCAGTAACAAATGTAAGTCAAGAAGATACTATGGTGATAAAGAAAGGTCCATGGACAGAAGAGGAGGATACTATTCTGATGAACTATGTGGCAGTCCACGGAGATGGTCACTGGAACTCCGTCGCTCGCTGTTCGGGTCTAAAGAGAACCGGGAAAAGCTGCAGATTAAGATGGTTGAACTACTTACGTCCAAATGTTCGTCGAGGGAACATCACTCTCCAAGAACAAGTCTTGATTCTTGATCTTCATTCCCGTTGGGGCAATCGGTGGTCTAAAATAGCTGAACAATTACCGGGAAGAACGGACAATGAAATAAAGAACTATTGGAGAACAAGAGTGGTGAAGCAAGCTAGGCAACTAAAATGTGATGTAAACAGTCAACAGTTTAGGGACACATTGCGTTTAGTATGGATTCCACGTCTCATCGAGAGAATCCAAACATCTCGTGCAACTGTAGATACTAATTCCTATAGCCACGGACAAATCACCACCTGCAACAATCCTAAAGCTCATAGTGAAACTTGTGGGGCAAATGCAAATCCTCCCATGTTGCAGTCAGAGGTTTCATCGTACTCGTCAGGGGTTGATGTTCAAGTAGAATCAAACGTATCTTACAATTTAATGGATTGTGCGGAACTGGGATCCACGTTGCAGGAGTGGAACTGCTTAGACTCAAACATCCAAGAATTTGAACAGAGCAATGTTTTTGGTGGTTCGGAATTGTGGACAGATGAAAATATATGGTTCTTGCAGCAGCAACTTGCTGATGATTTATGA

Protein sequence

>Msa0174020

MDTNYKTNIHVMRSFSDCGSSVTNVSQEDTMVIKKGPWTEEEDTILMNYVAVHGDGHWNSVARCSGLKRTGKSCRLRWLNYLRPNVRRGNITLQEQVLILDLHSRWGNRWSKIAEQLPGRTDNEIKNYWRTRVVKQARQLKCDVNSQQFRDTLRLVWIPRLIERIQTSRATVDTNSYSHGQITTCNNPKAHSETCGANANPPMLQSEVSSYSSGVDVQVESNVSYNLMDCAELGSTLQEWNCLDSNIQEFEQSNVFGGSELWTDENIWFLQQQLADDL*