AlfalfaGEDB | Msa0175250

M. sativa cultivar zhongmu-4 / Msa0175250

KEGG: -
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0175250	XP_024630769.2	95.000	60	3	0	87	146	315	374	2.03e-28	124
Msa0175250	XP_024630769.2	96.104	77	3	0	153	229	477	553	7.79e-39	152

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0175250	A0A072VK17	95.652	69	3	0	1	69	55	123	3.12e-38	139

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TFs/TRs:

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Gene ID	Type	Classification
Msa0175250	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0175250	MtrunA17_Chr3g0082121	76.056	71	17	0	1	71	64	134	3.62e-29	114
Msa0175250	MtrunA17_Chr3g0082121	52.326	86	33	2	140	223	614	693	1.65e-19	86.7
Msa0175250	MtrunA17_Chr3g0082121	72.131	61	17	0	86	146	458	518	2.25e-13	68.9
Msa0175250	MtrunA17_Chr5g0401121	67.606	71	23	0	1	71	77	147	6.34e-24	94.0
Msa0175250	MtrunA17_Chr6g0458391	42.373	59	34	0	2	60	247	305	4.38e-12	65.1
Msa0175250	MtrunA17_Chr1g0203721	39.130	69	41	1	3	71	86	153	4.66e-11	62.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 27 sgRNAs with CRISPR-Local

Find 260 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGGATGTCAATGCTAAA+CGG	0.487954	1_4:+61139253	Msa0175250:intron
AACAGGATGCACAGCGCGTA+TGG	0.489143	1_4:+61138197	Msa0175250:CDS
AAGATTCACATGCTTCAAAG+AGG	0.641966	1_4:+61138116	Msa0175250:CDS
AATGATTATAGTAAAAGAAT+TGG	0.319753	1_4:+61138033	None:intergenic
ACGCGCTGTGCATCCTGTTC+TGG	0.282615	1_4:-61138193	None:intergenic
AGATTCACATGCTTCAAAGA+GGG	0.653445	1_4:+61138117	Msa0175250:CDS
ATGAGATTCTGAAGCTCTAT+TGG	0.322184	1_4:-61139755	None:intergenic
ATTTGGAGTGTAAACATTTG+CGG	0.445050	1_4:-61139358	None:intergenic
CAAAAGACGTCAACCGACGG+CGG	0.706901	1_4:+61138155	Msa0175250:CDS
CAACATAGACACCGTTGGCA+AGG	0.578457	1_4:+61139918	Msa0175250:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GGAAATAAAAGCAGAATAGA+TGG	+	chr1_4:61138078-61138097	Msa0175250:CDS	30.0%
TCGAAAGATAGGGAAATATA+AGG	+	chr1_4:61138233-61138252	Msa0175250:CDS	30.0%
AGAATCATATGAACATACTC+AGG	-	chr1_4:61138300-61138319	None:intergenic	30.0%
TAAGCAAATTCTTAAGGTCT+TGG	-	chr1_4:61138456-61138475	None:intergenic	30.0%
GCACAGATGATTAATGATTA+TGG	+	chr1_4:61138619-61138638	Msa0175250:intron	30.0%
AAATAAGGATTATCGACCTT+TGG	+	chr1_4:61138684-61138703	Msa0175250:intron	30.0%
AATAAGGATTATCGACCTTT+GGG	+	chr1_4:61138685-61138704	Msa0175250:intron	30.0%
TTGTTGGACTTAATAATCAC+AGG	+	chr1_4:61138713-61138732	Msa0175250:intron	30.0%
GTTATGCCAAAAACATTTCA+TGG	+	chr1_4:61138889-61138908	Msa0175250:intron	30.0%
ATATAAGACAGAATGCTCTT+AGG	+	chr1_4:61138926-61138945	Msa0175250:intron	30.0%

Showing 1 to 10 of 260 entries

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	61138045	61139972	61138045	ID=Msa0175250;Name=Msa0175250
chr1_4	mRNA	61138045	61139972	61138045	ID=Msa0175250-mRNA-1;Parent=Msa0175250;Name=Msa0175250-mRNA-1;_AED=0.31;_eAED=0.43;_QI=0\|0\|0\|1\|0.25\|0\|5\|0\|229
chr1_4	exon	61138045	61138254	61138045	ID=Msa0175250-mRNA-1:exon:19476;Parent=Msa0175250-mRNA-1
chr1_4	exon	61139082	61139088	61139082	ID=Msa0175250-mRNA-1:exon:19477;Parent=Msa0175250-mRNA-1
chr1_4	exon	61139114	61139156	61139114	ID=Msa0175250-mRNA-1:exon:19478;Parent=Msa0175250-mRNA-1
chr1_4	exon	61139257	61139452	61139257	ID=Msa0175250-mRNA-1:exon:19479;Parent=Msa0175250-mRNA-1
chr1_4	exon	61139742	61139972	61139742	ID=Msa0175250-mRNA-1:exon:19480;Parent=Msa0175250-mRNA-1
chr1_4	CDS	61138045	61138254	61138045	ID=Msa0175250-mRNA-1:cds;Parent=Msa0175250-mRNA-1
chr1_4	CDS	61139082	61139088	61139082	ID=Msa0175250-mRNA-1:cds;Parent=Msa0175250-mRNA-1
chr1_4	CDS	61139114	61139156	61139114	ID=Msa0175250-mRNA-1:cds;Parent=Msa0175250-mRNA-1
chr1_4	CDS	61139257	61139452	61139257	ID=Msa0175250-mRNA-1:cds;Parent=Msa0175250-mRNA-1
chr1_4	CDS	61139742	61139972	61139742	ID=Msa0175250-mRNA-1:cds;Parent=Msa0175250-mRNA-1

Gene Sequence

>Msa0175250

AAAAGAATTGGATTTGGTATTCGTCGAGAATATGGAAATAAAAGCAGAATAGATGGAGTTTTGACTTCGAGAAGATTCACATGCTTCAAAGAGGGTGCGCAAGTTGTTGACAAAAGACGTCAACCGACGGCGGAGTCTAAAGCAGAAACCAGAACAGGATGCACAGCGCGTATGGTTATTTTACTTGATCGAAAGATAGGGAAATATAAGATTCGTGGAAAAATGGGCATAGGCTTATGTTCGAAAAACATTCACGGCAGGGATGTCAATGCTAAACGGAACAATGAGTCAAAAGCAGAATATGATTCAAGACATAAGCTGCCTAAATTGAAAATGAAGAAGGCTCGCATGCTCTTACAAGCCGCAAATGTTTACACTCCAAATTTTTTTGAGGAATTTCAAGAGGAATATGAAGAATATCAAGATACTTGCATAAAAGGAGGTGAAAGAAGGTTTTTGCTTAAATTGACCAATAGAGCTTCAGAATCTCATGAAACTTACACATTTTTGTCCAAAGTTTATGAAGAGTCCAACAAAATAGTTAATGATATGTTGTCCAAAAAATATCTGGATGGAGAATCAAGTGGAATGGTTCATGTATCCATATCAATTGCGAATGATGAAGTCGACAACAACATAGACACCGTTGGCAAGGCAAAATGCATAAAGATGAAGGATTGTCCACGT

Protein sequence

>Msa0175250

KRIGFGIRREYGNKSRIDGVLTSRRFTCFKEGAQVVDKRRQPTAESKAETRTGCTARMVILLDRKIGKYKIRGKMGIGLCSKNIHGRDVNAKRNNESKAEYDSRHKLPKLKMKKARMLLQAANVYTPNFFEEFQEEYEEYQDTCIKGGERRFLLKLTNRASESHETYTFLSKVYEESNKIVNDMLSKKYLDGESSGMVHVSISIANDEVDNNIDTVGKAKCIKMKDCPR