AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0178750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0178750 MtrunA17_Chr8g0364231 36.885 122 65 3 103 224 7 116 1.01e-14 72.8
Msa0178750 MtrunA17_Chr1g0206781 54.167 72 24 2 142 213 12 74 1.70e-14 67.8
Msa0178750 MtrunA17_Chr1g0205811 43.333 90 39 3 141 227 14 94 6.97e-14 66.2
Msa0178750 MtrunA17_Chr5g0415031 43.529 85 39 1 140 224 42 117 4.67e-13 68.2
Msa0178750 MtrunA17_Chr1g0206771 45.122 82 36 2 142 223 17 89 6.94e-13 64.7
Msa0178750 MtrunA17_Chr1g0206771 48.649 74 29 2 141 214 95 159 4.41e-11 59.7
Msa0178750 MtrunA17_Chr3g0077941 45.977 87 37 2 140 225 97 174 1.64e-12 63.5
Msa0178750 MtrunA17_Chr1g0205821 37.647 85 44 1 142 226 100 175 3.50e-12 62.8
Msa0178750 MtrunA17_Chr3g0077891 42.529 87 40 2 140 225 98 175 2.06e-11 60.8
Msa0178750 MtrunA17_Chr1g0205861 39.241 79 39 1 140 218 15 84 3.83e-11 58.5
Msa0178750 MtrunA17_Chr3g0077911 50.000 80 30 2 141 219 90 160 7.15e-11 58.9
Msa0178750 MtrunA17_Chr1g0209201 45.455 77 32 3 140 216 15 81 8.27e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0178750 AT1G24190.3 43.434 99 43 3 126 224 1 86 1.95e-13 69.7
Msa0178750 AT1G24190.1 43.434 99 43 3 126 224 1 86 2.01e-13 69.3
Msa0178750 AT1G24190.2 43.434 99 43 3 126 224 1 86 2.09e-13 69.3
Msa0178750 AT1G70060.2 43.434 99 43 3 126 224 1 86 6.70e-13 67.8
Msa0178750 AT1G70060.1 43.434 99 43 3 126 224 1 86 6.70e-13 67.8
Msa0178750 AT1G24200.1 45.238 84 37 1 140 223 13 87 1.09e-12 65.1
Msa0178750 AT1G59890.1 36.885 122 66 2 103 224 7 117 5.94e-12 65.1
Msa0178750 AT1G59890.2 36.885 122 66 2 103 224 7 117 6.00e-12 65.1
Msa0178750 AT1G59890.3 36.885 122 66 2 103 224 7 117 6.05e-12 65.1
Msa0178750 AT1G59890.4 36.885 122 66 2 103 224 7 117 7.02e-12 65.1

Find 54 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGCATACAAGTTTAATTT+TGG 0.105157 1_4:-66146002 Msa0178750:CDS
AAATACTCGTCATGCCTTTC+AGG 0.145655 1_4:+66146061 None:intergenic
TTCCCTTGAGTTTATGCTTC+CGG 0.297773 1_4:-66148515 None:intergenic
TTTAAAACGCGTGTTAGTTT+CGG 0.344738 1_4:-66148382 Msa0178750:CDS
CCAAGGCATCATTGTCATTT+CGG 0.348502 1_4:+66146188 None:intergenic
CCAGTGGTCTCAGGTTGATA+TGG 0.354996 1_4:+66145938 None:intergenic
CTGACATAGCTTACGAGATC+TGG 0.369893 1_4:-66146880 Msa0178750:intron
CATACCATTGCACTTGGGTT+TGG 0.400583 1_4:+66146235 None:intergenic
AAAAGAGTAGATCGAGTAGT+AGG 0.418518 1_4:+66146744 None:intergenic
CACTTGGGTTTGGCAGCTCG+CGG 0.434797 1_4:+66146245 None:intergenic
TGATGCCTTGGCGTATCTTA+AGG 0.437899 1_4:-66146176 Msa0178750:CDS
GCCACCGTCGGCACCGCCGC+TGG 0.449283 1_4:-66148360 Msa0178750:CDS
ACGACCTTCTTCCTTTCTCC+AGG 0.463722 1_4:-66148452 Msa0178750:CDS
ACTTGGGTTTGGCAGCTCGC+GGG 0.481398 1_4:+66146246 None:intergenic
TGAAACATCCACGGCGGCAC+CGG 0.483585 1_4:+66148496 None:intergenic
GTCATTTCGGAGGATCTTCA+AGG 0.487687 1_4:+66146201 None:intergenic
AATTACTAACCAGTGGTCTC+AGG 0.492768 1_4:+66145929 None:intergenic
TCATTACTCTTGCTTTGACC+AGG 0.493534 1_4:+66146035 None:intergenic
TTGAGAGGAAATTCAATTGG+TGG 0.499094 1_4:-66146795 Msa0178750:CDS
GATTTGAGAGGAAATTCAAT+TGG 0.504507 1_4:-66146798 Msa0178750:CDS
CCGAAATGACAATGATGCCT+TGG 0.512635 1_4:-66146188 Msa0178750:CDS
CGACAACGATGCCTGGAGAA+AGG 0.524280 1_4:+66148441 None:intergenic
AGCTCCTCCTCAGATTATCC+AGG 0.525195 1_4:-66148330 Msa0178750:intron
TGTGTGACGACAACGATGCC+TGG 0.529261 1_4:+66148434 None:intergenic
GAGAAGCATACCATTGCACT+TGG 0.531877 1_4:+66146229 None:intergenic
AAAATTCATTAAGGATTACC+AGG 0.537203 1_4:-66146104 Msa0178750:CDS
ATATCCAGCGGCGGTGCCGA+CGG 0.545007 1_4:+66148356 None:intergenic
TCCAGCGGCGGTGCCGACGG+TGG 0.553985 1_4:+66148359 None:intergenic
TTATGCTTCCGGTGCCGCCG+TGG 0.562978 1_4:-66148504 Msa0178750:CDS
CTCAAATCGCATGAATTCAA+AGG 0.563099 1_4:+66146813 None:intergenic
GTGTTAGTTTCGGCCACCGT+CGG 0.568017 1_4:-66148372 Msa0178750:CDS
TTATACGAATTATTCTAACC+TGG 0.580035 1_4:+66146086 None:intergenic
TTGAATTCATGCGATTTGAG+AGG 0.589951 1_4:-66146810 Msa0178750:CDS
CCATATCAACCTGAGACCAC+TGG 0.591406 1_4:-66145938 Msa0178750:CDS
AAGAAGGTCGTGTTTCTGAT+CGG 0.592690 1_4:+66148464 None:intergenic
GAATTCAAAGGCGTATAATG+AGG 0.605729 1_4:+66146825 None:intergenic
GAATTCAAAGGCGTATAATG+AGG 0.605729 1_4:+66146855 None:intergenic
TGAGGATCGCATGAATTCAA+AGG 0.612677 1_4:+66146843 None:intergenic
GCTGCCAAACCCAAGTGCAA+TGG 0.615126 1_4:-66146239 Msa0178750:CDS
GACTGCCTTAAGATACGCCA+AGG 0.621430 1_4:+66146171 None:intergenic
GGAGCTGCAATATCCAGCGG+CGG 0.629568 1_4:+66148347 None:intergenic
AGGCATCATTGTCATTTCGG+AGG 0.634269 1_4:+66146191 None:intergenic
GATGCCTGGAGAAAGGAAGA+AGG 0.635806 1_4:+66148448 None:intergenic
AGAAGCATACCATTGCACTT+GGG 0.639657 1_4:+66146230 None:intergenic
AACCCCACCTGGATAATCTG+AGG 0.644843 1_4:+66148323 None:intergenic
GCATGACGAGTATTTAAACC+TGG 0.646035 1_4:-66146053 Msa0178750:CDS
AGGCAAAGCTGAAACATCCA+CGG 0.647288 1_4:+66148487 None:intergenic
AATGATAAATTACTAACCAG+TGG 0.651186 1_4:+66145922 None:intergenic
GGAGGAGCTGCAATATCCAG+CGG 0.670837 1_4:+66148344 None:intergenic
TAATTCGTATAAGTCCTGAA+AGG 0.681985 1_4:-66146075 Msa0178750:CDS
AAGGTCGTGTTTCTGATCGG+AGG 0.688366 1_4:+66148467 None:intergenic
CCCACCTGGATAATCTGAGG+AGG 0.696292 1_4:+66148326 None:intergenic
ATTTCGGAGGATCTTCAAGG+AGG 0.728241 1_4:+66146204 None:intergenic
CAAAGCTGAAACATCCACGG+CGG 0.759000 1_4:+66148490 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATATATATATATAT+CGG - chr1_4:66147225-66147244 Msa0178750:intron 0.0%
!! TATATATATATATATATATA+GGG + chr1_4:66147224-66147243 None:intergenic 0.0%
!! ATATATATATATATATATAT+AGG + chr1_4:66147225-66147244 None:intergenic 0.0%
!! TATATATACATATATACATA+CGG + chr1_4:66147212-66147231 None:intergenic 10.0%
!!! TAAATTTTGTTACTAATTCA+TGG + chr1_4:66146200-66146219 None:intergenic 15.0%
!!! TTTGATTTTTACTAAATTGA+AGG + chr1_4:66146238-66146257 None:intergenic 15.0%
!! TACATGATTTCTAATTATAT+TGG - chr1_4:66146848-66146867 Msa0178750:CDS 15.0%
!! TTCCAAAAAACAAAAAAAAA+TGG - chr1_4:66147112-66147131 Msa0178750:intron 15.0%
!! ATATATACATATATACATAC+GGG + chr1_4:66147211-66147230 None:intergenic 15.0%
!!! TGAGTTTTTAAAATTCATTA+AGG - chr1_4:66148323-66148342 Msa0178750:intron 15.0%
!!! GTATTTTTGTCTAAAGTAAA+AGG - chr1_4:66146474-66146493 Msa0178750:intron 20.0%
!!! CTCCATTTTTTTTTGTTTTT+TGG + chr1_4:66147117-66147136 None:intergenic 20.0%
!! TATATACATATATACATACG+GGG + chr1_4:66147210-66147229 None:intergenic 20.0%
!!! AGTTTCAGATTTAAAATTGA+AGG + chr1_4:66147893-66147912 None:intergenic 20.0%
!! AATGATACAACAAATTAGTT+TGG - chr1_4:66147937-66147956 Msa0178750:intron 20.0%
!!! AAAATAACTTACTTTGAAGT+AGG - chr1_4:66148077-66148096 Msa0178750:intron 20.0%
! TTTCTAAATTCCTGCTTTAT+GGG + chr1_4:66146380-66146399 None:intergenic 25.0%
! ATTTCTAAATTCCTGCTTTA+TGG + chr1_4:66146381-66146400 None:intergenic 25.0%
! TTTGATTGAGTTCATAGAAT+GGG - chr1_4:66146722-66146741 Msa0178750:intron 25.0%
! TTGTATTAGTAACCTAGAAA+AGG + chr1_4:66146787-66146806 None:intergenic 25.0%
! TTTGGAAAAGACTAAATCAA+AGG + chr1_4:66147099-66147118 None:intergenic 25.0%
!! GTTTTTGTTCCAAAACAAAA+AGG - chr1_4:66147136-66147155 Msa0178750:intron 25.0%
! TGCTAAAATAGACCTTAATT+AGG - chr1_4:66147166-66147185 Msa0178750:intron 25.0%
! TAAAATAGACCTTAATTAGG+TGG - chr1_4:66147169-66147188 Msa0178750:intron 25.0%
! AAAATAGACCTTAATTAGGT+GGG - chr1_4:66147170-66147189 Msa0178750:intron 25.0%
! AAAATTCATTAAGGATTACC+AGG - chr1_4:66148332-66148351 Msa0178750:CDS 25.0%
! TTATACGAATTATTCTAACC+TGG + chr1_4:66148353-66148372 None:intergenic 25.0%
!!! AGAGCATACAAGTTTAATTT+TGG - chr1_4:66148434-66148453 Msa0178750:CDS 25.0%
!!! GAGCATACAAGTTTAATTTT+GGG - chr1_4:66148435-66148454 Msa0178750:CDS 25.0%
! TTTAAAACGCGTGTTAGTTT+CGG - chr1_4:66146054-66146073 Msa0178750:CDS 30.0%
TGTTACTAATTCATGGAATC+TGG + chr1_4:66146193-66146212 None:intergenic 30.0%
TATTCTCTTTGTTAGCTCAT+AGG + chr1_4:66146343-66146362 None:intergenic 30.0%
TAACAAAGAGAATAAGCTCT+AGG - chr1_4:66146349-66146368 Msa0178750:intron 30.0%
TTTGAGAAAGACAACATTGA+AGG + chr1_4:66146439-66146458 None:intergenic 30.0%
TAGACCTTCATAAAAATGAG+TGG - chr1_4:66146512-66146531 Msa0178750:intron 30.0%
AGACCTTCATAAAAATGAGT+GGG - chr1_4:66146513-66146532 Msa0178750:intron 30.0%
!! ATTTTGAAAACAGACCAGAA+GGG + chr1_4:66146637-66146656 None:intergenic 30.0%
!! TATTTTGAAAACAGACCAGA+AGG + chr1_4:66146638-66146657 None:intergenic 30.0%
GTTTGATTGAGTTCATAGAA+TGG - chr1_4:66146721-66146740 Msa0178750:intron 30.0%
!! TTGTGCGCTTTTGAAAAATT+TGG - chr1_4:66146907-66146926 Msa0178750:intron 30.0%
!! TGTGCGCTTTTGAAAAATTT+GGG - chr1_4:66146908-66146927 Msa0178750:intron 30.0%
! CTAATTTTGAGCTTCAGTTT+GGG + chr1_4:66147026-66147045 None:intergenic 30.0%
! TCTAATTTTGAGCTTCAGTT+TGG + chr1_4:66147027-66147046 None:intergenic 30.0%
ATAAAAGACACCTTCACAAA+AGG + chr1_4:66147350-66147369 None:intergenic 30.0%
!! ACACCAACTTACTTTTATGA+AGG - chr1_4:66147399-66147418 Msa0178750:intron 30.0%
ACACCTTCATAAAAGTAAGT+TGG + chr1_4:66147405-66147424 None:intergenic 30.0%
AAAGTGAAAATCACACCTTA+AGG - chr1_4:66147447-66147466 Msa0178750:intron 30.0%
! TTTTATCAAGCAACACCTTA+AGG + chr1_4:66147465-66147484 None:intergenic 30.0%
GATTTGAGAGGAAATTCAAT+TGG - chr1_4:66147638-66147657 Msa0178750:intron 30.0%
! ACTCGATCTACTCTTTTTAT+AGG - chr1_4:66147698-66147717 Msa0178750:intron 30.0%
!! TGAAGAAGCGTTTATGAAAA+GGG + chr1_4:66147859-66147878 None:intergenic 30.0%
TAATTCGTATAAGTCCTGAA+AGG - chr1_4:66148361-66148380 Msa0178750:CDS 30.0%
! AGTTTGAAACCAGTAGGAAT+AGG + chr1_4:66146153-66146172 None:intergenic 35.0%
!! AAAGAGAATAAGCTCTAGGT+GGG - chr1_4:66146353-66146372 Msa0178750:intron 35.0%
!! AGACCCACTCATTTTTATGA+AGG + chr1_4:66146519-66146538 None:intergenic 35.0%
! ATTCTCGTCAATCTAAAGCT+CGG + chr1_4:66146590-66146609 None:intergenic 35.0%
ATAGCTTGAATGTGTGTTTG+AGG - chr1_4:66146655-66146674 Msa0178750:intron 35.0%
TAGTACGAACTGAAATCAGA+AGG + chr1_4:66146694-66146713 None:intergenic 35.0%
GATTTCAGTTCGTACTACTA+TGG - chr1_4:66146697-66146716 Msa0178750:intron 35.0%
! AAGAGATGAGTACCTTTTCT+AGG - chr1_4:66146772-66146791 Msa0178750:CDS 35.0%
! ATTTCCTCCTCTTCTTTTAC+TGG - chr1_4:66146812-66146831 Msa0178750:CDS 35.0%
CTACAAACCAGTAAAAGAAG+AGG + chr1_4:66146822-66146841 None:intergenic 35.0%
!!! CTCTTCTTTTACTGGTTTGT+AGG - chr1_4:66146820-66146839 Msa0178750:CDS 35.0%
CAAAACCAAACTTCATTGGA+AGG + chr1_4:66146890-66146909 None:intergenic 35.0%
!!! CGTTCTCTTTCCTAGTTTTT+TGG + chr1_4:66146996-66147015 None:intergenic 35.0%
!! CAAGATGCACCTTTTTGTTT+TGG + chr1_4:66147148-66147167 None:intergenic 35.0%
ACAATAGACCCACCTAATTA+AGG + chr1_4:66147181-66147200 None:intergenic 35.0%
! ATAGAAGACACCTTTTGTGA+AGG - chr1_4:66147337-66147356 Msa0178750:intron 35.0%
TTGATAAAAGACACCACCAA+GGG - chr1_4:66147476-66147495 Msa0178750:intron 35.0%
! GAATTCAAAGGCGTATAATG+AGG + chr1_4:66147584-66147603 None:intergenic 35.0%
! GAATTCAAAGGCGTATAATG+AGG + chr1_4:66147584-66147603 None:intergenic 35.0%
CTCAAATCGCATGAATTCAA+AGG + chr1_4:66147626-66147645 None:intergenic 35.0%
TTGAATTCATGCGATTTGAG+AGG - chr1_4:66147626-66147645 Msa0178750:intron 35.0%
TTGAGAGGAAATTCAATTGG+TGG - chr1_4:66147641-66147660 Msa0178750:intron 35.0%
AAAAGAGTAGATCGAGTAGT+AGG + chr1_4:66147695-66147714 None:intergenic 35.0%
!! GTACGCATGTTTTTATTCAC+AGG - chr1_4:66147776-66147795 Msa0178750:intron 35.0%
!! AAGAAGCGTTTATGAAAAGG+GGG + chr1_4:66147857-66147876 None:intergenic 35.0%
!! GAAGAAGCGTTTATGAAAAG+GGG + chr1_4:66147858-66147877 None:intergenic 35.0%
!! CTGAAGAAGCGTTTATGAAA+AGG + chr1_4:66147860-66147879 None:intergenic 35.0%
! CTTTTCATAAACGCTTCTTC+AGG - chr1_4:66147858-66147877 Msa0178750:intron 35.0%
! AGCGTGAAATGATTTTGCTT+TGG + chr1_4:66148477-66148496 None:intergenic 35.0%
! AAGAAGGTCGTGTTTCTGAT+CGG + chr1_4:66145975-66145994 None:intergenic 40.0%
!!! TAACACGCGTTTTAAAGCAG+AGG + chr1_4:66146050-66146069 None:intergenic 40.0%
AAACCAGTAGGAATAGGAAG+GGG + chr1_4:66146147-66146166 None:intergenic 40.0%
GAAACCAGTAGGAATAGGAA+GGG + chr1_4:66146148-66146167 None:intergenic 40.0%
TGAAACCAGTAGGAATAGGA+AGG + chr1_4:66146149-66146168 None:intergenic 40.0%
! ATCGAGAGTTTGAAACCAGT+AGG + chr1_4:66146159-66146178 None:intergenic 40.0%
CATTTCTCTTGAATCGTTCC+AGG - chr1_4:66146301-66146320 Msa0178750:intron 40.0%
!! CAAAGAGAATAAGCTCTAGG+TGG - chr1_4:66146352-66146371 Msa0178750:intron 40.0%
AGTGGGTCTATTGTATATGC+TGG - chr1_4:66146530-66146549 Msa0178750:intron 40.0%
ACAAGAACATGTATCAGCAG+TGG + chr1_4:66146557-66146576 None:intergenic 40.0%
! TTCTCGTCAATCTAAAGCTC+GGG + chr1_4:66146589-66146608 None:intergenic 40.0%
TGTCCTTCTTTAATCCCTTC+TGG - chr1_4:66146620-66146639 Msa0178750:intron 40.0%
AGACCAGAAGGGATTAAAGA+AGG + chr1_4:66146626-66146645 None:intergenic 40.0%
CAAACCAGTAAAAGAAGAGG+AGG + chr1_4:66146819-66146838 None:intergenic 40.0%
TGCTTCCTTCCAATGAAGTT+TGG - chr1_4:66146882-66146901 Msa0178750:intron 40.0%
CGCACAAAACCAAACTTCAT+TGG + chr1_4:66146894-66146913 None:intergenic 40.0%
AAACTAGGAAAGAGAACGAG+TGG - chr1_4:66146998-66147017 Msa0178750:intron 40.0%
AACTAGGAAAGAGAACGAGT+GGG - chr1_4:66146999-66147018 Msa0178750:intron 40.0%
CTTGATAAAAGACACCACCA+AGG - chr1_4:66147475-66147494 Msa0178750:intron 40.0%
GTTATGTTACAAGACACCCT+TGG + chr1_4:66147495-66147514 None:intergenic 40.0%
TGAGGATCGCATGAATTCAA+AGG + chr1_4:66147596-66147615 None:intergenic 40.0%
CGTTTATGAAAAGGGGGTTA+GGG + chr1_4:66147851-66147870 None:intergenic 40.0%
! TGATTTGCAAAGTTGAGCAG+CGG + chr1_4:66148169-66148188 None:intergenic 40.0%
! AGAAGCATACCATTGCACTT+GGG + chr1_4:66148209-66148228 None:intergenic 40.0%
! CCAAGGCATCATTGTCATTT+CGG + chr1_4:66148251-66148270 None:intergenic 40.0%
AAATACTCGTCATGCCTTTC+AGG + chr1_4:66148378-66148397 None:intergenic 40.0%
GCATGACGAGTATTTAAACC+TGG - chr1_4:66148383-66148402 Msa0178750:CDS 40.0%
TCATTACTCTTGCTTTGACC+AGG + chr1_4:66148404-66148423 None:intergenic 40.0%
AGGCAAAGCTGAAACATCCA+CGG + chr1_4:66145952-66145971 None:intergenic 45.0%
GGCTTCTTCTCAATTCAACC+TGG + chr1_4:66146322-66146341 None:intergenic 45.0%
GTGACAGTGGCCAAAAAACT+AGG - chr1_4:66146983-66147002 Msa0178750:intron 45.0%
ATGTTACAAGACACCCTTGG+TGG + chr1_4:66147492-66147511 None:intergenic 45.0%
CTGACATAGCTTACGAGATC+TGG - chr1_4:66147556-66147575 Msa0178750:intron 45.0%
AGTTGGAGAATGCAGAGAAG+AGG + chr1_4:66147822-66147841 None:intergenic 45.0%
GCGTTTATGAAAAGGGGGTT+AGG + chr1_4:66147852-66147871 None:intergenic 45.0%
CATACCATTGCACTTGGGTT+TGG + chr1_4:66148204-66148223 None:intergenic 45.0%
! GAGAAGCATACCATTGCACT+TGG + chr1_4:66148210-66148229 None:intergenic 45.0%
ATTTCGGAGGATCTTCAAGG+AGG + chr1_4:66148235-66148254 None:intergenic 45.0%
GTCATTTCGGAGGATCTTCA+AGG + chr1_4:66148238-66148257 None:intergenic 45.0%
! AGGCATCATTGTCATTTCGG+AGG + chr1_4:66148248-66148267 None:intergenic 45.0%
CCGAAATGACAATGATGCCT+TGG - chr1_4:66148248-66148267 Msa0178750:intron 45.0%
TGATGCCTTGGCGTATCTTA+AGG - chr1_4:66148260-66148279 Msa0178750:intron 45.0%
CAAAGCTGAAACATCCACGG+CGG + chr1_4:66145949-66145968 None:intergenic 50.0%
! AAGGTCGTGTTTCTGATCGG+AGG + chr1_4:66145972-66145991 None:intergenic 50.0%
ACGACCTTCTTCCTTTCTCC+AGG - chr1_4:66145984-66146003 Msa0178750:CDS 50.0%
GATGCCTGGAGAAAGGAAGA+AGG + chr1_4:66145991-66146010 None:intergenic 50.0%
AGCTCCTCCTCAGATTATCC+AGG - chr1_4:66146106-66146125 Msa0178750:CDS 50.0%
TCCTCCTCAGATTATCCAGG+TGG - chr1_4:66146109-66146128 Msa0178750:CDS 50.0%
CCTCCTCAGATTATCCAGGT+GGG - chr1_4:66146110-66146129 Msa0178750:CDS 50.0%
CTCCTCAGATTATCCAGGTG+GGG - chr1_4:66146111-66146130 Msa0178750:CDS 50.0%
AACCCCACCTGGATAATCTG+AGG + chr1_4:66146116-66146135 None:intergenic 50.0%
AGAGAGAGAGAGAGTGACAG+TGG - chr1_4:66146970-66146989 Msa0178750:intron 50.0%
GACTGCCTTAAGATACGCCA+AGG + chr1_4:66148268-66148287 None:intergenic 50.0%
!! CCAGTGGTCTCAGGTTGATA+TGG + chr1_4:66148501-66148520 None:intergenic 50.0%
CCATATCAACCTGAGACCAC+TGG - chr1_4:66148498-66148517 Msa0178750:CDS 50.0%
CGACAACGATGCCTGGAGAA+AGG + chr1_4:66145998-66146017 None:intergenic 55.0%
TGTGTGACGACAACGATGCC+TGG + chr1_4:66146005-66146024 None:intergenic 55.0%
! GTGTTAGTTTCGGCCACCGT+CGG - chr1_4:66146064-66146083 Msa0178750:CDS 55.0%
GGAGGAGCTGCAATATCCAG+CGG + chr1_4:66146095-66146114 None:intergenic 55.0%
CCCACCTGGATAATCTGAGG+AGG + chr1_4:66146113-66146132 None:intergenic 55.0%
CAGCCCCTTCCTATTCCTAC+TGG - chr1_4:66146141-66146160 Msa0178750:CDS 55.0%
CTAGGTGGGACCCATAAAGC+AGG - chr1_4:66146367-66146386 Msa0178750:intron 55.0%
GGGGGTTAGGGTTCTTGAGT+TGG + chr1_4:66147839-66147858 None:intergenic 55.0%
CAAAGTTGAGCAGCGGCGAT+CGG + chr1_4:66148162-66148181 None:intergenic 55.0%
GCTGCCAAACCCAAGTGCAA+TGG - chr1_4:66148197-66148216 Msa0178750:intron 55.0%
TGAAACATCCACGGCGGCAC+CGG + chr1_4:66145943-66145962 None:intergenic 60.0%
GGAGCTGCAATATCCAGCGG+CGG + chr1_4:66146092-66146111 None:intergenic 60.0%
ACTTGGGTTTGGCAGCTCGC+GGG + chr1_4:66148193-66148212 None:intergenic 60.0%
CACTTGGGTTTGGCAGCTCG+CGG + chr1_4:66148194-66148213 None:intergenic 60.0%
! ATATCCAGCGGCGGTGCCGA+CGG + chr1_4:66146083-66146102 None:intergenic 65.0%
GGGCTGCGCAAAACCCCACC+TGG + chr1_4:66146127-66146146 None:intergenic 70.0%
!!! TCCAGCGGCGGTGCCGACGG+TGG + chr1_4:66146080-66146099 None:intergenic 80.0%
! GCCACCGTCGGCACCGCCGC+TGG - chr1_4:66146076-66146095 Msa0178750:CDS 85.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 66145934 66148524 66145934 ID=Msa0178750;Name=Msa0178750
chr1_4 mRNA 66145934 66148524 66145934 ID=Msa0178750-mRNA-1;Parent=Msa0178750;Name=Msa0178750-mRNA-1;_AED=0.54;_eAED=0.54;_QI=0|0.5|0|0.66|1|1|3|0|227
chr1_4 exon 66148331 66148524 66148331 ID=Msa0178750-mRNA-1:exon:21606;Parent=Msa0178750-mRNA-1
chr1_4 exon 66146739 66146893 66146739 ID=Msa0178750-mRNA-1:exon:21605;Parent=Msa0178750-mRNA-1
chr1_4 exon 66145934 66146268 66145934 ID=Msa0178750-mRNA-1:exon:21604;Parent=Msa0178750-mRNA-1
chr1_4 CDS 66148331 66148524 66148331 ID=Msa0178750-mRNA-1:cds;Parent=Msa0178750-mRNA-1
chr1_4 CDS 66146739 66146893 66146739 ID=Msa0178750-mRNA-1:cds;Parent=Msa0178750-mRNA-1
chr1_4 CDS 66145934 66146268 66145934 ID=Msa0178750-mRNA-1:cds;Parent=Msa0178750-mRNA-1
Gene Sequence

>Msa0178750

ATGCTTCCGGTGCCGCCGTGGATGTTTCAGCTTTGCCTCCGATCAGAAACACGACCTTCTTCCTTTCTCCAGGCATCGTTGTCGTCACACATAAAAATCGCGACTTTCTTCAACCTCTGCTTTAAAACGCGTGTTAGTTTCGGCCACCGTCGGCACCGCCGCTGGATATTGCAGCTCCTCCTCAGATTATCCAGCTTACGAGATCTGGATCCTCATTATACGCCTTTGAATTCATGCGATCCTCATTATACGCCTTTGAATTCATGCGATTTGAGAGGAAATTCAATTGGTGGAGACACAGCTTTTGAATGCTTCGTCTCTCCTACTACTCGATCTACTCTTTTTATAGCCCGCGAGCTGCCAAACCCAAGTGCAATGGTATGCTTCTCGTCCTCCTTGAAGATCCTCCGAAATGACAATGATGCCTTGGCGTATCTTAAGGCAGTCAAAGTTGAATTTCAATATAACACGAAAAAGTATAATGAGTTTTTAAAATTCATTAAGGATTACCAGGTTAGAATAATTCGTATAAGTCCTGAAAGGCATGACGAGTATTTAAACCTGGTCAAAGCAAGAGTAATGAAGCTGCTTAAAGAGCATACAAGTTTAATTTTGGGATTCAACATCTTTCTGCCAAAGCAAAATCATTTCACGCTTCCATATCAACCTGAGACCACTGGTTAG
Protein sequence

>Msa0178750

MLPVPPWMFQLCLRSETRPSSFLQASLSSHIKIATFFNLCFKTRVSFGHRRHRRWILQLLLRLSSLRDLDPHYTPLNSCDPHYTPLNSCDLRGNSIGGDTAFECFVSPTTRSTLFIARELPNPSAMVCFSSSLKILRNDNDALAYLKAVKVEFQYNTKKYNEFLKFIKDYQVRIIRISPERHDEYLNLVKARVMKLLKEHTSLILGFNIFLPKQNHFTLPYQPETTG*