AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0179240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0179240 MtrunA17_Chr1g0191141 83.423 1110 139 5 28 1105 7 1103 0.0 1882
Msa0179240 MtrunA17_Chr7g0224051 65.542 1126 302 15 55 1104 1 1116 0.0 1422
Msa0179240 MtrunA17_Chr3g0114161 70.444 856 199 10 300 1104 395 1247 0.0 1174
Msa0179240 MtrunA17_Chr3g0114161 64.746 295 90 4 28 308 24 318 3.95e-111 375
Msa0179240 MtrunA17_Chr5g0437621 65.259 734 238 10 382 1104 576 1303 0.0 942
Msa0179240 MtrunA17_Chr5g0437621 43.421 304 135 7 29 307 19 310 1.97e-55 211
Msa0179240 MtrunA17_Chr7g0225791 34.813 721 426 16 380 1085 577 1268 2.22e-115 387
Msa0179240 MtrunA17_Chr7g0233031 34.345 725 404 16 398 1091 549 1232 1.36e-114 385
Msa0179240 MtrunA17_Chr7g0233021 35.784 721 386 18 398 1085 537 1213 5.85e-114 382
Msa0179240 MtrunA17_Chr4g0047081 34.722 720 409 17 398 1095 548 1228 1.75e-112 379
Msa0179240 MtrunA17_Chr7g0232971 35.085 761 403 22 380 1100 584 1293 2.79e-112 379
Msa0179240 MtrunA17_Chr7g0224031 68.493 219 68 1 667 884 24 242 1.53e-96 306
Msa0179240 MtrunA17_Chr2g0296421 31.016 748 422 20 331 1060 330 1001 1.17e-91 317
Msa0179240 MtrunA17_Chr8g0352361 31.241 701 387 19 375 1060 232 852 3.69e-89 306
Msa0179240 MtrunA17_Chr7g0232991 33.280 628 342 16 506 1091 54 646 5.44e-89 300
Msa0179240 MtrunA17_Chr7g0233001 33.764 542 307 13 575 1095 3 513 7.24e-75 257
Msa0179240 MtrunA17_Chr7g0224021 70.536 112 31 1 495 606 2 111 1.67e-47 165
Msa0179240 MtrunA17_Chr2g0320781 37.247 247 139 7 813 1053 4 240 4.97e-39 145
Msa0179240 MtrunA17_Chr8g0384531 25.087 578 327 21 512 1052 372 880 1.87e-29 127
Msa0179240 MtrunA17_Chr5g0393461 24.329 633 371 24 487 1090 163 716 2.23e-29 126
Msa0179240 MtrunA17_Chr5g0403921 24.821 560 329 19 559 1103 206 688 1.49e-23 107
Msa0179240 MtrunA17_Chr1g0190391 27.731 238 144 6 518 751 576 789 7.78e-22 102
Msa0179240 MtrunA17_Chr3g0137091 25.050 499 296 16 558 1043 310 743 4.38e-20 97.1
Msa0179240 MtrunA17_Chr1g0190381 35.526 152 88 3 923 1065 205 355 9.28e-19 91.3
Msa0179240 MtrunA17_Chr5g0394201 23.346 544 329 20 559 1092 385 850 1.24e-15 82.4
Msa0179240 MtrunA17_Chr5g0442141 22.374 514 309 18 551 1049 633 1071 8.71e-15 79.7
Msa0179240 MtrunA17_Chr3g0095091 24.423 520 297 20 551 1052 587 1028 1.53e-14 79.0
Msa0179240 MtrunA17_Chr4g0066741 23.017 517 299 21 559 1062 210 640 4.37e-14 77.4
Msa0179240 MtrunA17_Chr4g0040301 31.138 167 102 4 915 1074 389 549 6.06e-14 76.6
Msa0179240 MtrunA17_Chr2g0286291 23.183 509 308 18 559 1062 213 643 1.93e-13 75.1
Msa0179240 MtrunA17_Chr3g0114111 88.235 34 4 0 1058 1091 1 34 1.71e-12 63.9
Msa0179240 MtrunA17_Chr7g0257101 27.778 198 135 5 865 1061 1151 1341 7.93e-12 70.1
Msa0179240 MtrunA17_Chr7g0255821 39.362 94 56 1 951 1043 690 783 3.62e-11 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0179240 AT3G42670.2 61.646 717 257 9 398 1105 548 1255 0.0 894
Msa0179240 AT3G42670.2 37.102 283 150 7 29 298 18 285 2.52e-42 169
Msa0179240 AT3G42670.3 61.646 717 257 9 398 1105 548 1255 0.0 894
Msa0179240 AT3G42670.3 37.102 283 150 7 29 298 18 285 2.52e-42 169
Msa0179240 AT3G42670.4 61.646 717 259 9 398 1105 548 1257 0.0 894
Msa0179240 AT3G42670.4 37.102 283 150 7 29 298 18 285 2.87e-42 169
Msa0179240 AT3G42670.1 61.646 717 257 9 398 1105 548 1255 0.0 894
Msa0179240 AT3G42670.1 37.102 283 150 7 29 298 18 285 2.52e-42 169
Msa0179240 AT5G20420.1 57.588 771 308 9 342 1105 503 1261 0.0 874
Msa0179240 AT5G20420.1 34.448 299 149 6 29 302 18 294 2.81e-42 169
Msa0179240 AT1G05490.1 32.964 722 422 18 398 1089 695 1384 6.12e-105 360
Msa0179240 AT3G24340.1 32.222 720 421 15 387 1093 449 1114 4.99e-102 348
Msa0179240 AT2G16390.2 31.909 702 384 19 376 1060 226 850 4.61e-88 304
Msa0179240 AT2G16390.1 31.909 702 384 19 376 1060 223 847 5.43e-88 304
Msa0179240 AT2G21450.3 32.055 652 358 20 433 1060 92 682 1.03e-84 291
Msa0179240 AT2G21450.2 31.748 652 360 19 433 1060 190 780 3.77e-84 291
Msa0179240 AT2G21450.1 31.748 652 360 19 433 1060 183 773 4.91e-84 291
Msa0179240 AT3G19210.2 23.980 588 357 21 524 1084 180 704 2.76e-27 120
Msa0179240 AT3G19210.1 24.407 590 354 22 524 1084 180 706 4.48e-27 119
Msa0179240 AT3G19210.3 24.407 590 354 22 524 1084 180 706 5.27e-27 119
Msa0179240 AT5G63950.2 24.417 557 315 23 524 1052 377 855 1.39e-24 112
Msa0179240 AT5G63950.1 24.643 560 327 23 524 1052 377 872 1.64e-24 111
Msa0179240 AT1G08600.4 26.866 335 189 12 524 849 719 1006 9.97e-24 109
Msa0179240 AT1G08600.4 34.969 163 94 4 912 1065 1123 1282 1.05e-17 89.7
Msa0179240 AT1G08600.3 26.866 335 189 12 524 849 719 1006 9.97e-24 109
Msa0179240 AT1G08600.3 34.969 163 94 4 912 1065 1123 1282 1.05e-17 89.7
Msa0179240 AT1G08600.2 26.866 335 189 12 524 849 719 1006 9.97e-24 109
Msa0179240 AT1G08600.2 34.969 163 94 4 912 1065 1123 1282 1.05e-17 89.7
Msa0179240 AT5G44800.1 25.992 504 285 21 555 1043 707 1137 2.96e-21 101
Msa0179240 AT4G31900.3 23.801 542 302 19 514 1043 214 656 9.29e-20 96.3
Msa0179240 AT4G31900.2 23.801 542 302 19 514 1043 173 615 9.88e-20 96.3
Msa0179240 AT4G31900.1 23.801 542 302 19 514 1043 214 656 1.06e-19 96.3
Msa0179240 AT1G08600.1 34.969 163 94 4 912 1065 1102 1261 1.04e-17 89.7
Msa0179240 AT1G08600.1 24.024 333 180 9 524 849 719 985 7.22e-17 87.0
Msa0179240 AT2G13370.1 23.092 511 301 18 558 1052 646 1080 3.64e-15 81.6
Msa0179240 AT2G13370.2 23.092 511 301 18 558 1052 646 1080 3.64e-15 81.6
Msa0179240 AT2G13370.3 23.092 511 301 18 558 1052 646 1080 3.64e-15 81.6
Msa0179240 AT1G03750.1 30.682 176 108 5 907 1074 528 697 6.15e-15 80.5
Msa0179240 AT2G44980.1 25.097 518 298 22 565 1066 80 523 5.32e-13 73.9
Msa0179240 AT3G57300.1 30.114 176 115 5 887 1061 1187 1355 1.33e-12 73.2
Msa0179240 AT3G57300.4 30.114 176 115 5 887 1061 1187 1355 1.33e-12 73.2
Msa0179240 AT3G57300.2 30.114 176 115 5 887 1061 1220 1388 1.58e-12 72.8
Msa0179240 AT2G18760.4 31.387 137 88 3 924 1060 745 875 1.05e-11 70.1
Msa0179240 AT2G18760.1 31.387 137 88 3 924 1060 745 875 1.05e-11 70.1
Msa0179240 AT2G18760.2 31.387 137 88 3 924 1060 745 875 1.05e-11 70.1
Msa0179240 AT2G18760.7 31.387 137 88 3 924 1060 745 875 1.05e-11 70.1
Msa0179240 AT2G18760.5 31.387 137 88 3 924 1060 745 875 1.05e-11 70.1
Msa0179240 AT2G18760.6 31.387 137 88 3 924 1060 745 875 1.05e-11 70.1
Msa0179240 AT2G18760.3 31.387 137 88 3 924 1060 804 934 1.06e-11 70.1
Msa0179240 AT5G66750.1 29.940 167 112 2 924 1090 542 703 2.95e-11 68.2

Find 162 sgRNAs with CRISPR-Local

Find 253 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTTGTTTCAAAACAATTT+TGG 0.098097 1_4:+66663087 Msa0179240:CDS
TAAGAGAAGGTGGTTGTTTA+AGG 0.177258 1_4:+66661180 Msa0179240:CDS
CCAGGCAGAACCATTGATTT+TGG 0.203037 1_4:-66662781 None:intergenic
AGAACTTCGTGAATAAATTT+TGG 0.216798 1_4:-66663141 None:intergenic
TGCTTGACACCATTAGAATT+TGG 0.218550 1_4:-66662808 None:intergenic
AACTTTGATGTGGTACTTCT+AGG 0.221583 1_4:-66663012 None:intergenic
CTTATCTTCTGTTCTCATTA+AGG 0.232667 1_4:-66662911 None:intergenic
TATCGATTGGATGAACAAAT+TGG 0.242680 1_4:+66661923 Msa0179240:CDS
ATATGGATTTGATAGCTTTA+TGG 0.245952 1_4:+66661206 Msa0179240:CDS
AGACCTCCAATTTCGCTTCT+TGG 0.261495 1_4:-66661037 None:intergenic
TCGACCTGGTCAAGAGAAAA+TGG 0.262902 1_4:+66663965 Msa0179240:CDS
ACATCAAGTTATCTTCTTGA+TGG 0.278990 1_4:+66661508 Msa0179240:CDS
AGTTATTTCGTTGAAGTCTT+TGG 0.292757 1_4:-66660703 None:intergenic
GGTAGGTGACAAGGCTAAAA+TGG 0.298106 1_4:+66662293 Msa0179240:intron
TAATGGTGTCAAGCATGATT+TGG 0.304673 1_4:+66662816 Msa0179240:CDS
TATGGATTTGATAGCTTTAT+GGG 0.316460 1_4:+66661207 Msa0179240:CDS
CCAATGCCAAGAAGCGAAAT+TGG 0.318140 1_4:+66661031 Msa0179240:CDS
CCAAAGAAAATGATAGTGTT+TGG 0.318198 1_4:+66662419 Msa0179240:CDS
TTGAAGTTCAAAACATTCAT+AGG 0.322383 1_4:-66660956 None:intergenic
CAAGCTTGTGCATCAAGTTA+TGG 0.328101 1_4:+66660910 Msa0179240:CDS
AACAAATTTGCTTTGAGAAA+AGG 0.336871 1_4:+66663594 Msa0179240:CDS
TTGGTGTCGTTGAATGTATC+TGG 0.339974 1_4:-66662762 None:intergenic
ATACGATAATAGCGCTATAA+TGG 0.343852 1_4:+66659838 Msa0179240:CDS
AGTTCTTCTGATACTCTTCC+TGG 0.348560 1_4:+66663360 Msa0179240:CDS
AAAGTTGAGGAAGTGCTTAA+TGG 0.350370 1_4:+66663029 Msa0179240:CDS
GCAGAAACTGCAAAAGAAAA+TGG 0.357505 1_4:+66663437 Msa0179240:CDS
GCTCTTCATTGCCACAATCT+AGG 0.360216 1_4:+66660795 Msa0179240:CDS
CCAATTTCGCTTCTTGGCAT+TGG 0.361638 1_4:-66661031 None:intergenic
AAAATTTATTCACGAAGTTC+TGG 0.379765 1_4:+66663143 Msa0179240:CDS
TTCATAGGACTACTAATATT+AGG 0.382189 1_4:-66660941 None:intergenic
GTGGTTGTTTAAGGAGCATA+TGG 0.383291 1_4:+66661189 Msa0179240:CDS
ATATGTCAATTCCAGGTCTT+AGG 0.387141 1_4:-66660337 None:intergenic
AAGCTTGTGCATCAAGTTAT+GGG 0.390339 1_4:+66660911 Msa0179240:CDS
AGGTGGTGATAATGTTTCTT+CGG 0.390951 1_4:+66661126 Msa0179240:CDS
CATGTGTAGAGTGTGGTCTT+TGG 0.395080 1_4:-66662676 None:intergenic
GCTCAAAACAAATCTTACTT+TGG 0.400942 1_4:-66663619 None:intergenic
GGTGGTGATAATGTTTCTTC+GGG 0.402375 1_4:+66661127 Msa0179240:CDS
AATTCCAGGTCTTAGGAAAC+TGG 0.403179 1_4:-66660330 None:intergenic
CCTGGAGTTCCAAATTCTAA+TGG 0.403216 1_4:+66662799 Msa0179240:CDS
AAGAAAATGGCGGGCTGTAC+CGG 0.405933 1_4:+66663450 Msa0179240:CDS
CAAAGAAAATGATAGTGTTT+GGG 0.407919 1_4:+66662420 Msa0179240:CDS
GGTAGAAAATCCTGATGATT+TGG 0.409165 1_4:+66661453 Msa0179240:CDS
GTCTTTGGAGCTAGGACTAA+TGG 0.409580 1_4:-66662661 None:intergenic
AAAGAGATTCTGCAACTAAC+TGG 0.420585 1_4:+66663753 Msa0179240:CDS
CTCAACCTCAAGAGAGTAAC+CGG 0.423138 1_4:-66663469 None:intergenic
ATCATGTTCACATGTTTCCT+TGG 0.426716 1_4:-66661900 None:intergenic
CACCCTAGTGTTCTTGTCAT+GGG 0.427375 1_4:+66662871 Msa0179240:CDS
TCAACTGATGCTACTGATCT+TGG 0.430086 1_4:+66662385 Msa0179240:CDS
ACGCGCGAGAAAATTCTTCT+TGG 0.431056 1_4:+66663230 Msa0179240:CDS
AAGGAAGAGGATAATGTGAT+TGG 0.437162 1_4:+66660659 Msa0179240:CDS
TACGTAATGTTCAAGTTGTA+TGG 0.439233 1_4:-66661080 None:intergenic
AAGAAAAGAGATGATGAGAA+AGG 0.450749 1_4:-66662605 None:intergenic
ACACCCTAGTGTTCTTGTCA+TGG 0.450907 1_4:+66662870 Msa0179240:CDS
TCTATATTTATCTTCCTCCA+TGG 0.451446 1_4:-66664018 None:intergenic
TTGAACTTCAAATCAGAAAA+CGG 0.451497 1_4:+66660968 Msa0179240:CDS
CTGATGATTTGGCTGGCATA+TGG 0.454793 1_4:+66661464 Msa0179240:CDS
AGCACAGATATGTCAATTCC+AGG 0.458031 1_4:-66660344 None:intergenic
ATATCTCATGCTCCTGGAGC+TGG 0.462480 1_4:+66662577 Msa0179240:CDS
TACTATGAGTTTGTTGTGCA+TGG 0.462547 1_4:-66660253 None:intergenic
TCAATCAATGCTTGTTCTGA+AGG 0.465693 1_4:+66663855 Msa0179240:CDS
TATCGATGATCATGAAGAAC+AGG 0.466478 1_4:+66661276 Msa0179240:CDS
GCATTTGAAGCAGTATTGAA+TGG 0.467351 1_4:+66660170 Msa0179240:intron
AGTAAAATTCTCAATGCTGC+TGG 0.475556 1_4:-66663057 None:intergenic
GTATATCACTTGTTGATGAC+TGG 0.488667 1_4:+66663993 Msa0179240:CDS
AGAGTGTGGTCTTTGGAGCT+AGG 0.489103 1_4:-66662669 None:intergenic
CACAATCTCGTGTTGTTCAT+CGG 0.491623 1_4:-66663415 None:intergenic
CCATTAGAATTTGGAACTCC+AGG 0.495262 1_4:-66662799 None:intergenic
TACCGCGTCATCTTTAAAGA+AGG 0.495527 1_4:+66660859 Msa0179240:CDS
CATATGCCAGCCAAATCATC+AGG 0.497067 1_4:-66661463 None:intergenic
ACGTGTGATGAGCAAGGAAA+AGG 0.497284 1_4:+66663650 Msa0179240:CDS
AATCTTCTCAGCTTGCCATT+CGG 0.505551 1_4:-66664105 None:intergenic
TATTTCGTTGAAGTCTTTGG+AGG 0.508106 1_4:-66660700 None:intergenic
AGATTGTAATGATGAAAAGT+TGG 0.511319 1_4:+66661102 Msa0179240:CDS
GTAATTTGTCATCAGACATC+CGG 0.513031 1_4:+66660273 Msa0179240:CDS
GCGCATGGCTAAAGCATTGA+GGG 0.513248 1_4:+66662951 Msa0179240:CDS
AGCGCTATAATGGATATTCA+TGG 0.513605 1_4:+66659848 Msa0179240:CDS
TGCTCCAGTTTCCTAAGACC+TGG 0.515668 1_4:+66660326 Msa0179240:CDS
CATGAAATCGACATGAACAT+TGG 0.516088 1_4:-66660586 None:intergenic
CTTGTTGATGACTGGATCCA+TGG 0.517661 1_4:+66664001 Msa0179240:CDS
GAAGAACTACTTGGAAAGAG+TGG 0.517977 1_4:+66664039 Msa0179240:CDS
AAGCAGTATTGAATGGCTCA+TGG 0.520661 1_4:+66660177 Msa0179240:CDS
GAAACAATGCAGTGAAGATA+CGG 0.522442 1_4:+66662327 Msa0179240:CDS
CCAAAATCAATGGTTCTGCC+TGG 0.525654 1_4:+66662781 Msa0179240:CDS
TGTGTTATATCTCATGCTCC+TGG 0.525908 1_4:+66662571 Msa0179240:CDS
TTATCTTCTTGATGGAAATG+AGG 0.528274 1_4:+66661516 Msa0179240:CDS
TGGATAAGATACACCGGCAC+GGG 0.534854 1_4:-66662727 None:intergenic
GGTTGCATTTGTCATAAGAT+CGG 0.534985 1_4:+66660880 Msa0179240:CDS
TATGATGATCAAGGTTCACT+TGG 0.538490 1_4:+66660617 Msa0179240:CDS
TTCAACGACACCAAAATCAA+TGG 0.539455 1_4:+66662771 Msa0179240:CDS
CTGTACCGGTTACTCTCTTG+AGG 0.542553 1_4:+66663464 Msa0179240:CDS
TGATGATTTGGCTGGCATAT+GGG 0.542896 1_4:+66661465 Msa0179240:CDS
TTCTCAGCTTGCCATTCGGA+AGG 0.544538 1_4:-66664101 None:intergenic
TTGTGCATGGAGGAAATGTA+TGG 0.544715 1_4:-66660240 None:intergenic
CGTATCGAAAAGCGGTGATG+AGG 0.549235 1_4:+66661432 Msa0179240:CDS
GAAAATCCTGATGATTTGGC+TGG 0.557990 1_4:+66661457 Msa0179240:CDS
GTCTAGAAAAGATCAAAAGC+TGG 0.560297 1_4:+66662842 Msa0179240:CDS
ATAAATATAGAAGAACTACT+TGG 0.563098 1_4:+66664030 Msa0179240:CDS
TTAGATTCTAAGTACAGAAG+AGG 0.564016 1_4:+66663171 Msa0179240:CDS
AGATTGAAGATGAAATACTC+AGG 0.564407 1_4:+66664123 Msa0179240:CDS
TGACACAAATAAAGAATCCA+AGG 0.564564 1_4:+66661883 Msa0179240:CDS
AATGTATAGATTTGCAAACC+AGG 0.566512 1_4:-66663378 None:intergenic
AAGAACTACTTGGAAAGAGT+GGG 0.567137 1_4:+66664040 Msa0179240:CDS
GCGCGTGCCTCTAGTAAATG+AGG 0.568077 1_4:-66663213 None:intergenic
TCATCAAGTATCAAGAGTCC+AGG 0.569191 1_4:-66662979 None:intergenic
TAACCCATGACAAGAACACT+AGG 0.569323 1_4:-66662874 None:intergenic
AGCGCATGGCTAAAGCATTG+AGG 0.574680 1_4:+66662950 Msa0179240:CDS
TGCTCATCACACGTGAGATT+AGG 0.575173 1_4:-66663641 None:intergenic
GAACATTGGTTATATTTGTG+AGG 0.579746 1_4:-66660572 None:intergenic
AAACTGCAAAAGAAAATGGC+GGG 0.587027 1_4:+66663441 Msa0179240:CDS
ATATCCAATGGTAGGTGACA+AGG 0.588447 1_4:+66662284 Msa0179240:intron
AGCAGTATTGAATGGCTCAT+GGG 0.590169 1_4:+66660178 Msa0179240:CDS
AAAGCATTGAGGGAAAGTCC+TGG 0.590693 1_4:+66662961 Msa0179240:CDS
GCACTTCCTCAACTTTGATG+TGG 0.590805 1_4:-66663022 None:intergenic
CCGAAAAGTAACCTAGATTG+TGG 0.592635 1_4:-66660806 None:intergenic
AGATGAAATACTCAGGGAGA+TGG 0.595348 1_4:+66664130 Msa0179240:CDS
CTTCAACGAAATAACTGTGA+AGG 0.595665 1_4:+66660710 Msa0179240:CDS
CAAAGAAGCTTAAGAGAAGG+TGG 0.596830 1_4:+66661170 Msa0179240:CDS
ATGCCAAGAAGCGAAATTGG+AGG 0.597152 1_4:+66661034 Msa0179240:CDS
AAAATGGAATATTCCCGTGC+CGG 0.598414 1_4:+66662714 Msa0179240:CDS
AGCATCGATTACTTCCTCGC+AGG 0.600178 1_4:-66661332 None:intergenic
GATAAATTTGAAGATCGTTG+TGG 0.605781 1_4:+66663810 Msa0179240:CDS
TGGACTCTTGATACTTGATG+AGG 0.608858 1_4:+66662981 Msa0179240:CDS
GGAATATACTGCCGTACATG+CGG 0.610029 1_4:+66661944 Msa0179240:CDS
ACTCAGGGAGATGGTTGAAG+AGG 0.610175 1_4:+66664139 Msa0179240:CDS
AGATGACGCGGTAACAAACC+AGG 0.611437 1_4:-66660849 None:intergenic
GCAGGGTGCAAATGCTGATG+AGG 0.611917 1_4:+66661853 Msa0179240:intron
CAAAGACCACACTCTACACA+TGG 0.613075 1_4:+66662677 Msa0179240:CDS
CACTCGGGTCAATACACCCA+TGG 0.615844 1_4:+66663502 Msa0179240:CDS
CAGAAGAGGCAAACTAGAGG+AGG 0.618526 1_4:+66663185 Msa0179240:CDS
AAATTCACACAGAAAGCGCA+TGG 0.619203 1_4:+66662936 Msa0179240:CDS
GAGCATGATTATGACGGAGA+AGG 0.621402 1_4:+66660998 Msa0179240:CDS
AATCTCACGTGTGATGAGCA+AGG 0.622148 1_4:+66663644 Msa0179240:CDS
TCACTTGAGCATGATTATGA+CGG 0.623465 1_4:+66660992 Msa0179240:CDS
GATTGAAGATGAAATACTCA+GGG 0.623647 1_4:+66664124 Msa0179240:CDS
CATGAAGAACAGGATCATAG+AGG 0.626014 1_4:+66661286 Msa0179240:CDS
GAAGCGATACAATCCAAAGA+AGG 0.629502 1_4:+66661229 Msa0179240:CDS
AATGAACTCAAATATTGATG+CGG 0.633014 1_4:+66663269 Msa0179240:CDS
ATGTGAACATGATTATCGAT+TGG 0.633134 1_4:+66661910 Msa0179240:CDS
GAAACTGCAAAAGAAAATGG+CGG 0.634136 1_4:+66663440 Msa0179240:CDS
GTATCTGGCTTCGACAACTG+TGG 0.634216 1_4:-66662747 None:intergenic
GTACAGAAGAGGCAAACTAG+AGG 0.636952 1_4:+66663182 Msa0179240:CDS
GGACTCTTGATACTTGATGA+GGG 0.637751 1_4:+66662982 Msa0179240:CDS
GCACAAAGAAGCTTAAGAGA+AGG 0.639100 1_4:+66661167 Msa0179240:CDS
GAATGGCTCATGGGAACCTG+TGG 0.639123 1_4:+66660187 Msa0179240:CDS
AAAGAGAAATCTGACACAAG+TGG 0.641751 1_4:+66662544 Msa0179240:CDS
GTCACAACCTTGCACCAGTG+AGG 0.646068 1_4:+66663685 Msa0179240:CDS
TGTAATGATGAAAAGTTGGA+AGG 0.659731 1_4:+66661106 Msa0179240:CDS
GAAGTACCACATCAAAGTTG+AGG 0.659873 1_4:+66663016 Msa0179240:CDS
GTGGATAAGATACACCGGCA+CGG 0.662416 1_4:-66662728 None:intergenic
TTGTTGAAGATCCTTCCGAA+TGG 0.668864 1_4:+66664090 Msa0179240:CDS
AACCTTCTTTAAAGATGACG+CGG 0.674091 1_4:-66660861 None:intergenic
AAAGAAAACGTATCGAAAAG+CGG 0.683079 1_4:+66661424 Msa0179240:CDS
GTCTACGACGCTCAAAACGT+AGG 0.683290 1_4:+66661056 Msa0179240:CDS
GAGAAATCTGACACAAGTGG+TGG 0.685073 1_4:+66662547 Msa0179240:CDS
AACCCATGACAAGAACACTA+GGG 0.686530 1_4:-66662873 None:intergenic
CAACTGTGGATAAGATACAC+CGG 0.690332 1_4:-66662733 None:intergenic
AATGATGAAAAGTTGGAAGG+TGG 0.696462 1_4:+66661109 Msa0179240:CDS
GGAAGCGCCTCATTTACTAG+AGG 0.697989 1_4:+66663206 Msa0179240:CDS
TATGAGTTTGTTGTGCATGG+AGG 0.703519 1_4:-66660250 None:intergenic
ATGACGGAGAAGGTGCAATG+CGG 0.752637 1_4:+66661008 Msa0179240:CDS
GTTAAATGCTGATACCTGCG+AGG 0.758695 1_4:+66661318 Msa0179240:CDS
GTTGATGACTGGATCCATGG+AGG 0.768053 1_4:+66664004 Msa0179240:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAATAAATGTATACAGA+AGG - chr1_4:66660472-66660491 None:intergenic 15.0%
!!! GTAAAACATTTTATACATAT+AGG - chr1_4:66661622-66661641 None:intergenic 15.0%
!! ATATGACAATTATGATTTAT+AGG + chr1_4:66661717-66661736 Msa0179240:intron 15.0%
!!! GATTTAAATACGTTATTTTT+TGG + chr1_4:66661774-66661793 Msa0179240:intron 15.0%
!! AGTTTATGTTAATGAATTAC+CGG - chr1_4:66659943-66659962 None:intergenic 20.0%
!! TGTTTAGAAATAAGAATTAG+AGG - chr1_4:66660150-66660169 None:intergenic 20.0%
!!! AACTCATAAAAATTGATTCT+GGG + chr1_4:66660210-66660229 Msa0179240:CDS 20.0%
!!! TGAAGTTAATTCTTTTAGAA+GGG + chr1_4:66660757-66660776 Msa0179240:CDS 20.0%
!!! TTTTTTACTTGACTTACAAT+GGG - chr1_4:66662004-66662023 None:intergenic 20.0%
!!! TAATTACAAGTTTGAAATCA+CGG - chr1_4:66662160-66662179 None:intergenic 20.0%
!! TGTAAATTAAGTTCCTTATT+TGG + chr1_4:66662184-66662203 Msa0179240:intron 20.0%
!!! TTGTTACATATTTTCTCTAT+TGG + chr1_4:66662250-66662269 Msa0179240:intron 20.0%
!!! ACTTTGTTTCAAAACAATTT+TGG + chr1_4:66663087-66663106 Msa0179240:CDS 20.0%
!!! CTTTGTTTCAAAACAATTTT+GGG + chr1_4:66663088-66663107 Msa0179240:CDS 20.0%
!!! TTTGTTTCAAAACAATTTTG+GGG + chr1_4:66663089-66663108 Msa0179240:CDS 20.0%
!! ATAAATATAGAAGAACTACT+TGG + chr1_4:66664030-66664049 Msa0179240:CDS 20.0%
!! GTATGATATATTTAAGATGC+CGG + chr1_4:66659921-66659940 Msa0179240:intron 25.0%
! AATGTAATTGACAATCTTGT+AGG + chr1_4:66660051-66660070 Msa0179240:intron 25.0%
!! GAACTCATAAAAATTGATTC+TGG + chr1_4:66660209-66660228 Msa0179240:CDS 25.0%
!! GTAAACTTTTATGATGATCA+AGG + chr1_4:66660608-66660627 Msa0179240:CDS 25.0%
!! CTGAAGTTAATTCTTTTAGA+AGG + chr1_4:66660756-66660775 Msa0179240:CDS 25.0%
! TTCATAGGACTACTAATATT+AGG - chr1_4:66660944-66660963 None:intergenic 25.0%
! TTGAAGTTCAAAACATTCAT+AGG - chr1_4:66660959-66660978 None:intergenic 25.0%
! TTGAACTTCAAATCAGAAAA+CGG + chr1_4:66660968-66660987 Msa0179240:CDS 25.0%
! AGATTGTAATGATGAAAAGT+TGG + chr1_4:66661102-66661121 Msa0179240:CDS 25.0%
!! ATATGGATTTGATAGCTTTA+TGG + chr1_4:66661206-66661225 Msa0179240:CDS 25.0%
!!! TATGGATTTGATAGCTTTAT+GGG + chr1_4:66661207-66661226 Msa0179240:CDS 25.0%
!! TATTTGACTTCAACCATAAA+TGG - chr1_4:66661688-66661707 None:intergenic 25.0%
! TGACAATTATGATTTATAGG+CGG + chr1_4:66661720-66661739 Msa0179240:intron 25.0%
!!! TGGTTTTATCGTCTATTTTA+AGG + chr1_4:66661794-66661813 Msa0179240:intron 25.0%
! AACTATTAAATACCATAGCA+GGG + chr1_4:66661836-66661855 Msa0179240:intron 25.0%
!!! GTTTTTTACTTGACTTACAA+TGG - chr1_4:66662005-66662024 None:intergenic 25.0%
!! AATTAAGTTCCTTATTTGGT+TGG + chr1_4:66662188-66662207 Msa0179240:intron 25.0%
! AAAACATATCCAACCAAATA+AGG - chr1_4:66662200-66662219 None:intergenic 25.0%
! GTTGTCTAATTTATATCCAA+TGG + chr1_4:66662272-66662291 Msa0179240:intron 25.0%
! TCTAATTTATATCCAATGGT+AGG + chr1_4:66662276-66662295 Msa0179240:intron 25.0%
!! CTATCATTTTCTTTGGATAT+AGG - chr1_4:66662415-66662434 None:intergenic 25.0%
!! CAAAGAAAATGATAGTGTTT+GGG + chr1_4:66662420-66662439 Msa0179240:CDS 25.0%
!! AGAACTTCGTGAATAAATTT+TGG - chr1_4:66663144-66663163 None:intergenic 25.0%
! AAAATTTATTCACGAAGTTC+TGG + chr1_4:66663143-66663162 Msa0179240:CDS 25.0%
! AATGAACTCAAATATTGATG+CGG + chr1_4:66663269-66663288 Msa0179240:CDS 25.0%
!!! AACAAATTTGCTTTGAGAAA+AGG + chr1_4:66663594-66663613 Msa0179240:CDS 25.0%
!! TATTTGAGAATCATTTTCAG+TGG + chr1_4:66663721-66663740 Msa0179240:CDS 25.0%
!! AATCATTTTCAGTGGAAAAA+TGG + chr1_4:66663729-66663748 Msa0179240:CDS 25.0%
ATACGATAATAGCGCTATAA+TGG + chr1_4:66659838-66659857 Msa0179240:CDS 30.0%
! TCAATTTTTATGAGTTCCAC+AGG - chr1_4:66660206-66660225 None:intergenic 30.0%
! TAGCTTTTCTTGATCTTATC+CGG - chr1_4:66660295-66660314 None:intergenic 30.0%
GTAAGAAGATTGAACTTACT+GGG - chr1_4:66660409-66660428 None:intergenic 30.0%
AGTAAGAAGATTGAACTTAC+TGG - chr1_4:66660410-66660429 None:intergenic 30.0%
!!! TTTGTGAGGTTTTTTGTGTA+TGG - chr1_4:66660561-66660580 None:intergenic 30.0%
! GAACATTGGTTATATTTGTG+AGG - chr1_4:66660575-66660594 None:intergenic 30.0%
CACTGAAATAAAAACTCTGA+AGG + chr1_4:66660640-66660659 Msa0179240:CDS 30.0%
! AATAAAAACTCTGAAGGAAG+AGG + chr1_4:66660646-66660665 Msa0179240:CDS 30.0%
! AGTTATTTCGTTGAAGTCTT+TGG - chr1_4:66660706-66660725 None:intergenic 30.0%
!! TACGTAATGTTCAAGTTGTA+TGG - chr1_4:66661083-66661102 None:intergenic 30.0%
TGTAATGATGAAAAGTTGGA+AGG + chr1_4:66661106-66661125 Msa0179240:CDS 30.0%
! TTTCTTTTGAACACCTTCTT+TGG - chr1_4:66661245-66661264 None:intergenic 30.0%
TATCTTTCTTCACTGATAGT+TGG - chr1_4:66661391-66661410 None:intergenic 30.0%
AAAGAAAACGTATCGAAAAG+CGG + chr1_4:66661424-66661443 Msa0179240:CDS 30.0%
ACATCAAGTTATCTTCTTGA+TGG + chr1_4:66661508-66661527 Msa0179240:CDS 30.0%
TTATCTTCTTGATGGAAATG+AGG + chr1_4:66661516-66661535 Msa0179240:CDS 30.0%
ATTGACTGTTGAACCATTTA+TGG + chr1_4:66661672-66661691 Msa0179240:intron 30.0%
!!! ATCGTCTATTTTAAGGCTAA+AGG + chr1_4:66661801-66661820 Msa0179240:intron 30.0%
CAACTATTAAATACCATAGC+AGG + chr1_4:66661835-66661854 Msa0179240:intron 30.0%
TGACACAAATAAAGAATCCA+AGG + chr1_4:66661883-66661902 Msa0179240:CDS 30.0%
ATGTGAACATGATTATCGAT+TGG + chr1_4:66661910-66661929 Msa0179240:CDS 30.0%
! TATCGATTGGATGAACAAAT+TGG + chr1_4:66661923-66661942 Msa0179240:CDS 30.0%
!!! GTTTTAATTAGCAACGAGTT+TGG + chr1_4:66662044-66662063 Msa0179240:intron 30.0%
TAACTTTCAGAAAATCACAG+TGG - chr1_4:66662085-66662104 None:intergenic 30.0%
! CCAAACACTATCATTTTCTT+TGG - chr1_4:66662422-66662441 None:intergenic 30.0%
! CCAAAGAAAATGATAGTGTT+TGG + chr1_4:66662419-66662438 Msa0179240:CDS 30.0%
!! AAAAAGCTTTCGAGTTTCTT+TGG + chr1_4:66662485-66662504 Msa0179240:CDS 30.0%
! TTTCTTTGGAAAAACATTGC+AGG + chr1_4:66662499-66662518 Msa0179240:CDS 30.0%
! TTCTTTGGAAAAACATTGCA+GGG + chr1_4:66662500-66662519 Msa0179240:CDS 30.0%
AAGAAAAGAGATGATGAGAA+AGG - chr1_4:66662608-66662627 None:intergenic 30.0%
CTTATCTTCTGTTCTCATTA+AGG - chr1_4:66662914-66662933 None:intergenic 30.0%
! GCATTGAGAATTTTACTTTC+TGG + chr1_4:66663063-66663082 Msa0179240:CDS 30.0%
!! TTCGTGAATAAATTTTGGTC+TGG - chr1_4:66663139-66663158 None:intergenic 30.0%
TTAGATTCTAAGTACAGAAG+AGG + chr1_4:66663171-66663190 Msa0179240:CDS 30.0%
TATTGATGCGGAAAAAATGA+AGG + chr1_4:66663281-66663300 Msa0179240:CDS 30.0%
ATTGATGCGGAAAAAATGAA+GGG + chr1_4:66663282-66663301 Msa0179240:CDS 30.0%
AATGTATAGATTTGCAAACC+AGG - chr1_4:66663381-66663400 None:intergenic 30.0%
!!! TAGTTTTTTCAACTCTTCAC+CGG - chr1_4:66663568-66663587 None:intergenic 30.0%
GCTCAAAACAAATCTTACTT+TGG - chr1_4:66663622-66663641 None:intergenic 30.0%
AACTCTATTAAAAACCTCAC+TGG - chr1_4:66663702-66663721 None:intergenic 30.0%
GATAAATTTGAAGATCGTTG+TGG + chr1_4:66663810-66663829 Msa0179240:CDS 30.0%
!!! ATAGCTTGCTTTGTTTTTGA+AGG - chr1_4:66663933-66663952 None:intergenic 30.0%
TCTATATTTATCTTCCTCCA+TGG - chr1_4:66664021-66664040 None:intergenic 30.0%
! TTCTTGTATGATTTTCAGTG+AGG + chr1_4:66664064-66664083 Msa0179240:CDS 30.0%
AGATTGAAGATGAAATACTC+AGG + chr1_4:66664123-66664142 Msa0179240:CDS 30.0%
GATTGAAGATGAAATACTCA+GGG + chr1_4:66664124-66664143 Msa0179240:CDS 30.0%
!! AGCGCTATAATGGATATTCA+TGG + chr1_4:66659848-66659867 Msa0179240:CDS 35.0%
TAACTAGATGTGCTATGCAT+TGG + chr1_4:66660001-66660020 Msa0179240:intron 35.0%
! GCATTTGAAGCAGTATTGAA+TGG + chr1_4:66660170-66660189 Msa0179240:intron 35.0%
!! TACTATGAGTTTGTTGTGCA+TGG - chr1_4:66660256-66660275 None:intergenic 35.0%
! GTAATTTGTCATCAGACATC+CGG + chr1_4:66660273-66660292 Msa0179240:CDS 35.0%
ATATGTCAATTCCAGGTCTT+AGG - chr1_4:66660340-66660359 None:intergenic 35.0%
CATGAAATCGACATGAACAT+TGG - chr1_4:66660589-66660608 None:intergenic 35.0%
TATGATGATCAAGGTTCACT+TGG + chr1_4:66660617-66660636 Msa0179240:CDS 35.0%
AAGGAAGAGGATAATGTGAT+TGG + chr1_4:66660659-66660678 Msa0179240:CDS 35.0%
TATTTCGTTGAAGTCTTTGG+AGG - chr1_4:66660703-66660722 None:intergenic 35.0%
CTTCAACGAAATAACTGTGA+AGG + chr1_4:66660710-66660729 Msa0179240:CDS 35.0%
!!! CCACAATCTAGGTTACTTTT+CGG + chr1_4:66660806-66660825 Msa0179240:CDS 35.0%
!!! CACAATCTAGGTTACTTTTC+GGG + chr1_4:66660807-66660826 Msa0179240:CDS 35.0%
! AGTTTTTGTCTGATCTTTCC+TGG + chr1_4:66660831-66660850 Msa0179240:CDS 35.0%
! AACCTTCTTTAAAGATGACG+CGG - chr1_4:66660864-66660883 None:intergenic 35.0%
GGTTGCATTTGTCATAAGAT+CGG + chr1_4:66660880-66660899 Msa0179240:CDS 35.0%
AAGCTTGTGCATCAAGTTAT+GGG + chr1_4:66660911-66660930 Msa0179240:CDS 35.0%
TCACTTGAGCATGATTATGA+CGG + chr1_4:66660992-66661011 Msa0179240:CDS 35.0%
AATGATGAAAAGTTGGAAGG+TGG + chr1_4:66661109-66661128 Msa0179240:CDS 35.0%
! AGGTGGTGATAATGTTTCTT+CGG + chr1_4:66661126-66661145 Msa0179240:CDS 35.0%
TAAGAGAAGGTGGTTGTTTA+AGG + chr1_4:66661180-66661199 Msa0179240:CDS 35.0%
TATCGATGATCATGAAGAAC+AGG + chr1_4:66661276-66661295 Msa0179240:CDS 35.0%
TGAAGAAAGATAGTTTGAGC+AGG + chr1_4:66661399-66661418 Msa0179240:intron 35.0%
GGTAGAAAATCCTGATGATT+TGG + chr1_4:66661453-66661472 Msa0179240:CDS 35.0%
TGACAAGTAAAAGACGAGAT+GGG - chr1_4:66661563-66661582 None:intergenic 35.0%
ATGACAAGTAAAAGACGAGA+TGG - chr1_4:66661564-66661583 None:intergenic 35.0%
ATCATGTTCACATGTTTCCT+TGG - chr1_4:66661903-66661922 None:intergenic 35.0%
! TTACAAGTTTGAAATCACGG+TGG - chr1_4:66662157-66662176 None:intergenic 35.0%
GAAACAATGCAGTGAAGATA+CGG + chr1_4:66662327-66662346 Msa0179240:CDS 35.0%
!! TGCATGTTTTCTTTGAGTTC+AGG - chr1_4:66662454-66662473 None:intergenic 35.0%
!!! GAAACTCGAAAGCTTTTTTC+TGG - chr1_4:66662483-66662502 None:intergenic 35.0%
AAAGAGAAATCTGACACAAG+TGG + chr1_4:66662544-66662563 Msa0179240:CDS 35.0%
GGCAAAAAGAGTTCGAAAAA+TGG + chr1_4:66662698-66662717 Msa0179240:CDS 35.0%
TTCAACGACACCAAAATCAA+TGG + chr1_4:66662771-66662790 Msa0179240:CDS 35.0%
TGCTTGACACCATTAGAATT+TGG - chr1_4:66662811-66662830 None:intergenic 35.0%
! TAATGGTGTCAAGCATGATT+TGG + chr1_4:66662816-66662835 Msa0179240:CDS 35.0%
GTCTAGAAAAGATCAAAAGC+TGG + chr1_4:66662842-66662861 Msa0179240:CDS 35.0%
AACTTTGATGTGGTACTTCT+AGG - chr1_4:66663015-66663034 None:intergenic 35.0%
!! AAAGTTGAGGAAGTGCTTAA+TGG + chr1_4:66663029-66663048 Msa0179240:CDS 35.0%
AGTAAAATTCTCAATGCTGC+TGG - chr1_4:66663060-66663079 None:intergenic 35.0%
GTGCTTCGAAAAATCACAAA+TGG + chr1_4:66663312-66663331 Msa0179240:CDS 35.0%
GCAGAAACTGCAAAAGAAAA+TGG + chr1_4:66663437-66663456 Msa0179240:CDS 35.0%
GAAACTGCAAAAGAAAATGG+CGG + chr1_4:66663440-66663459 Msa0179240:CDS 35.0%
AAACTGCAAAAGAAAATGGC+GGG + chr1_4:66663441-66663460 Msa0179240:CDS 35.0%
!! GTTGAGCTTTTGATAACACT+CGG + chr1_4:66663486-66663505 Msa0179240:CDS 35.0%
!! TTGAGCTTTTGATAACACTC+GGG + chr1_4:66663487-66663506 Msa0179240:CDS 35.0%
AAAGAGATTCTGCAACTAAC+TGG + chr1_4:66663753-66663772 Msa0179240:CDS 35.0%
TCAATCAATGCTTGTTCTGA+AGG + chr1_4:66663855-66663874 Msa0179240:CDS 35.0%
! AGCTTCAAGAGTGATTTTCT+TGG + chr1_4:66663893-66663912 Msa0179240:CDS 35.0%
! TGATTTTCTTGGATTCTGAG+TGG + chr1_4:66663904-66663923 Msa0179240:CDS 35.0%
! TAAACCATTTTCTCTTGACC+AGG - chr1_4:66663972-66663991 None:intergenic 35.0%
!! GTATATCACTTGTTGATGAC+TGG + chr1_4:66663993-66664012 Msa0179240:CDS 35.0%
AAGAACTACTTGGAAAGAGT+GGG + chr1_4:66664040-66664059 Msa0179240:CDS 35.0%
GATACATGTGTCTAACTCGT+TGG + chr1_4:66659883-66659902 Msa0179240:intron 40.0%
! AAGCAGTATTGAATGGCTCA+TGG + chr1_4:66660177-66660196 Msa0179240:CDS 40.0%
! AGCAGTATTGAATGGCTCAT+GGG + chr1_4:66660178-66660197 Msa0179240:CDS 40.0%
TTGTGCATGGAGGAAATGTA+TGG - chr1_4:66660243-66660262 None:intergenic 40.0%
!! TATGAGTTTGTTGTGCATGG+AGG - chr1_4:66660253-66660272 None:intergenic 40.0%
AATTCCAGGTCTTAGGAAAC+TGG - chr1_4:66660333-66660352 None:intergenic 40.0%
AGCACAGATATGTCAATTCC+AGG - chr1_4:66660347-66660366 None:intergenic 40.0%
CCGAAAAGTAACCTAGATTG+TGG - chr1_4:66660809-66660828 None:intergenic 40.0%
! TACCGCGTCATCTTTAAAGA+AGG + chr1_4:66660859-66660878 Msa0179240:CDS 40.0%
CAAGCTTGTGCATCAAGTTA+TGG + chr1_4:66660910-66660929 Msa0179240:CDS 40.0%
! GGTGGTGATAATGTTTCTTC+GGG + chr1_4:66661127-66661146 Msa0179240:CDS 40.0%
GCACAAAGAAGCTTAAGAGA+AGG + chr1_4:66661167-66661186 Msa0179240:CDS 40.0%
CAAAGAAGCTTAAGAGAAGG+TGG + chr1_4:66661170-66661189 Msa0179240:CDS 40.0%
! GTGGTTGTTTAAGGAGCATA+TGG + chr1_4:66661189-66661208 Msa0179240:CDS 40.0%
GAAGCGATACAATCCAAAGA+AGG + chr1_4:66661229-66661248 Msa0179240:CDS 40.0%
CATGAAGAACAGGATCATAG+AGG + chr1_4:66661286-66661305 Msa0179240:CDS 40.0%
GGAAGTAATCGATGCTTACA+TGG + chr1_4:66661339-66661358 Msa0179240:intron 40.0%
TGATAGTTGGATCTTCTGGT+AGG - chr1_4:66661378-66661397 None:intergenic 40.0%
TCACTGATAGTTGGATCTTC+TGG - chr1_4:66661382-66661401 None:intergenic 40.0%
! GAAAATCCTGATGATTTGGC+TGG + chr1_4:66661457-66661476 Msa0179240:CDS 40.0%
! TGATGATTTGGCTGGCATAT+GGG + chr1_4:66661465-66661484 Msa0179240:CDS 40.0%
! TTTTCACAAAGCCGCATGTA+CGG - chr1_4:66661958-66661977 None:intergenic 40.0%
ATATCCAATGGTAGGTGACA+AGG + chr1_4:66662284-66662303 Msa0179240:intron 40.0%
TCAACTGATGCTACTGATCT+TGG + chr1_4:66662385-66662404 Msa0179240:CDS 40.0%
GAAAAACATTGCAGGGTCTA+TGG + chr1_4:66662507-66662526 Msa0179240:CDS 40.0%
TGTGTTATATCTCATGCTCC+TGG + chr1_4:66662571-66662590 Msa0179240:CDS 40.0%
! AGGACTAATGGTCTTTTCTG+TGG - chr1_4:66662652-66662671 None:intergenic 40.0%
! TTTTTGCCATGTGTAGAGTG+TGG - chr1_4:66662686-66662705 None:intergenic 40.0%
AAAATGGAATATTCCCGTGC+CGG + chr1_4:66662714-66662733 Msa0179240:CDS 40.0%
CAACTGTGGATAAGATACAC+CGG - chr1_4:66662736-66662755 None:intergenic 40.0%
!! TTGGTGTCGTTGAATGTATC+TGG - chr1_4:66662765-66662784 None:intergenic 40.0%
! CCATTAGAATTTGGAACTCC+AGG - chr1_4:66662802-66662821 None:intergenic 40.0%
CCTGGAGTTCCAAATTCTAA+TGG + chr1_4:66662799-66662818 Msa0179240:CDS 40.0%
AACCCATGACAAGAACACTA+GGG - chr1_4:66662876-66662895 None:intergenic 40.0%
TAACCCATGACAAGAACACT+AGG - chr1_4:66662877-66662896 None:intergenic 40.0%
! AAATTCACACAGAAAGCGCA+TGG + chr1_4:66662936-66662955 Msa0179240:CDS 40.0%
TCATCAAGTATCAAGAGTCC+AGG - chr1_4:66662982-66663001 None:intergenic 40.0%
TGGACTCTTGATACTTGATG+AGG + chr1_4:66662981-66663000 Msa0179240:CDS 40.0%
GGACTCTTGATACTTGATGA+GGG + chr1_4:66662982-66663001 Msa0179240:CDS 40.0%
! GAAGTACCACATCAAAGTTG+AGG + chr1_4:66663016-66663035 Msa0179240:CDS 40.0%
AGTTCTTCTGATACTCTTCC+TGG + chr1_4:66663360-66663379 Msa0179240:CDS 40.0%
! CACAATCTCGTGTTGTTCAT+CGG - chr1_4:66663418-66663437 None:intergenic 40.0%
!!! CTGCTGTTTTGATCAACCAT+GGG - chr1_4:66663521-66663540 None:intergenic 40.0%
!!! TCTGCTGTTTTGATCAACCA+TGG - chr1_4:66663522-66663541 None:intergenic 40.0%
! TGTGCAGCTAAGTTTTTCTC+CGG + chr1_4:66663546-66663565 Msa0179240:CDS 40.0%
TTAAAAACCTCACTGGTGCA+AGG - chr1_4:66663695-66663714 None:intergenic 40.0%
GAAGAACTACTTGGAAAGAG+TGG + chr1_4:66664039-66664058 Msa0179240:CDS 40.0%
TTGTTGAAGATCCTTCCGAA+TGG + chr1_4:66664090-66664109 Msa0179240:CDS 40.0%
AATCTTCTCAGCTTGCCATT+CGG - chr1_4:66664108-66664127 None:intergenic 40.0%
AGATGAAATACTCAGGGAGA+TGG + chr1_4:66664130-66664149 Msa0179240:CDS 40.0%
GCTCTTCATTGCCACAATCT+AGG + chr1_4:66660795-66660814 Msa0179240:CDS 45.0%
! GAGCATGATTATGACGGAGA+AGG + chr1_4:66660998-66661017 Msa0179240:CDS 45.0%
CCAATTTCGCTTCTTGGCAT+TGG - chr1_4:66661034-66661053 None:intergenic 45.0%
CCAATGCCAAGAAGCGAAAT+TGG + chr1_4:66661031-66661050 Msa0179240:CDS 45.0%
ATGCCAAGAAGCGAAATTGG+AGG + chr1_4:66661034-66661053 Msa0179240:CDS 45.0%
AGACCTCCAATTTCGCTTCT+TGG - chr1_4:66661040-66661059 None:intergenic 45.0%
GTTAAATGCTGATACCTGCG+AGG + chr1_4:66661318-66661337 Msa0179240:CDS 45.0%
CATATGCCAGCCAAATCATC+AGG - chr1_4:66661466-66661485 None:intergenic 45.0%
! CTGATGATTTGGCTGGCATA+TGG + chr1_4:66661464-66661483 Msa0179240:CDS 45.0%
GGAATATACTGCCGTACATG+CGG + chr1_4:66661944-66661963 Msa0179240:CDS 45.0%
! GTTTGAAATCACGGTGGATC+TGG - chr1_4:66662151-66662170 None:intergenic 45.0%
!! TTAGCCTTGTCACCTACCAT+TGG - chr1_4:66662291-66662310 None:intergenic 45.0%
GGTAGGTGACAAGGCTAAAA+TGG + chr1_4:66662293-66662312 Msa0179240:intron 45.0%
GAGAAATCTGACACAAGTGG+TGG + chr1_4:66662547-66662566 Msa0179240:CDS 45.0%
! AGAAAGGTTTTTCCAGCTCC+AGG - chr1_4:66662592-66662611 None:intergenic 45.0%
!! GTCTTTGGAGCTAGGACTAA+TGG - chr1_4:66662664-66662683 None:intergenic 45.0%
CATGTGTAGAGTGTGGTCTT+TGG - chr1_4:66662679-66662698 None:intergenic 45.0%
CAAAGACCACACTCTACACA+TGG + chr1_4:66662677-66662696 Msa0179240:CDS 45.0%
!!! CCAGGCAGAACCATTGATTT+TGG - chr1_4:66662784-66662803 None:intergenic 45.0%
! CCAAAATCAATGGTTCTGCC+TGG + chr1_4:66662781-66662800 Msa0179240:CDS 45.0%
ACACCCTAGTGTTCTTGTCA+TGG + chr1_4:66662870-66662889 Msa0179240:CDS 45.0%
CACCCTAGTGTTCTTGTCAT+GGG + chr1_4:66662871-66662890 Msa0179240:CDS 45.0%
!! AAAGCATTGAGGGAAAGTCC+TGG + chr1_4:66662961-66662980 Msa0179240:CDS 45.0%
GCACTTCCTCAACTTTGATG+TGG - chr1_4:66663025-66663044 None:intergenic 45.0%
GTACAGAAGAGGCAAACTAG+AGG + chr1_4:66663182-66663201 Msa0179240:CDS 45.0%
ACGCGCGAGAAAATTCTTCT+TGG + chr1_4:66663230-66663249 Msa0179240:CDS 45.0%
CTCAACCTCAAGAGAGTAAC+CGG - chr1_4:66663472-66663491 None:intergenic 45.0%
TGCTCATCACACGTGAGATT+AGG - chr1_4:66663644-66663663 None:intergenic 45.0%
AATCTCACGTGTGATGAGCA+AGG + chr1_4:66663644-66663663 Msa0179240:CDS 45.0%
ACGTGTGATGAGCAAGGAAA+AGG + chr1_4:66663650-66663669 Msa0179240:CDS 45.0%
TCGACCTGGTCAAGAGAAAA+TGG + chr1_4:66663965-66663984 Msa0179240:CDS 45.0%
!! CTTGTTGATGACTGGATCCA+TGG + chr1_4:66664001-66664020 Msa0179240:CDS 45.0%
TGCTCCAGTTTCCTAAGACC+TGG + chr1_4:66660326-66660345 Msa0179240:CDS 50.0%
AGATGACGCGGTAACAAACC+AGG - chr1_4:66660852-66660871 None:intergenic 50.0%
! ATGACGGAGAAGGTGCAATG+CGG + chr1_4:66661008-66661027 Msa0179240:CDS 50.0%
GTCTACGACGCTCAAAACGT+AGG + chr1_4:66661056-66661075 Msa0179240:CDS 50.0%
!! AGCATCGATTACTTCCTCGC+AGG - chr1_4:66661335-66661354 None:intergenic 50.0%
CGTATCGAAAAGCGGTGATG+AGG + chr1_4:66661432-66661451 Msa0179240:CDS 50.0%
TCAGCATTTGCACCCTGCTA+TGG - chr1_4:66661851-66661870 None:intergenic 50.0%
ATATCTCATGCTCCTGGAGC+TGG + chr1_4:66662577-66662596 Msa0179240:CDS 50.0%
!! AGAGTGTGGTCTTTGGAGCT+AGG - chr1_4:66662672-66662691 None:intergenic 50.0%
TGGATAAGATACACCGGCAC+GGG - chr1_4:66662730-66662749 None:intergenic 50.0%
GTGGATAAGATACACCGGCA+CGG - chr1_4:66662731-66662750 None:intergenic 50.0%
! GTATCTGGCTTCGACAACTG+TGG - chr1_4:66662750-66662769 None:intergenic 50.0%
!! AGCGCATGGCTAAAGCATTG+AGG + chr1_4:66662950-66662969 Msa0179240:CDS 50.0%
!! GCGCATGGCTAAAGCATTGA+GGG + chr1_4:66662951-66662970 Msa0179240:CDS 50.0%
CAGAAGAGGCAAACTAGAGG+AGG + chr1_4:66663185-66663204 Msa0179240:CDS 50.0%
!! GGAAGCGCCTCATTTACTAG+AGG + chr1_4:66663206-66663225 Msa0179240:CDS 50.0%
!! AAGAAAATGGCGGGCTGTAC+CGG + chr1_4:66663450-66663469 Msa0179240:CDS 50.0%
!! CTGTACCGGTTACTCTCTTG+AGG + chr1_4:66663464-66663483 Msa0179240:CDS 50.0%
!! GTTGATGACTGGATCCATGG+AGG + chr1_4:66664004-66664023 Msa0179240:CDS 50.0%
TTCTCAGCTTGCCATTCGGA+AGG - chr1_4:66664104-66664123 None:intergenic 50.0%
ACTCAGGGAGATGGTTGAAG+AGG + chr1_4:66664139-66664158 Msa0179240:CDS 50.0%
GAATGGCTCATGGGAACCTG+TGG + chr1_4:66660187-66660206 Msa0179240:CDS 55.0%
GCAGGGTGCAAATGCTGATG+AGG + chr1_4:66661853-66661872 Msa0179240:intron 55.0%
GCGCGTGCCTCTAGTAAATG+AGG - chr1_4:66663216-66663235 None:intergenic 55.0%
CACTCGGGTCAATACACCCA+TGG + chr1_4:66663502-66663521 Msa0179240:CDS 55.0%
GTCACAACCTTGCACCAGTG+AGG + chr1_4:66663685-66663704 Msa0179240:CDS 55.0%
! ATTGCACGCGCTTTTCGACC+TGG + chr1_4:66663951-66663970 Msa0179240:CDS 55.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 66659797 66664217 66659797 ID=Msa0179240;Name=Msa0179240
chr1_4 mRNA 66659797 66664217 66659797 ID=Msa0179240-mRNA-1;Parent=Msa0179240;Name=Msa0179240-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|0|0|0.83|1|1|6|0|1105
chr1_4 exon 66659797 66659878 66659797 ID=Msa0179240-mRNA-1:exon:21828;Parent=Msa0179240-mRNA-1
chr1_4 exon 66660171 66660409 66660171 ID=Msa0179240-mRNA-1:exon:21829;Parent=Msa0179240-mRNA-1
chr1_4 exon 66660533 66661342 66660533 ID=Msa0179240-mRNA-1:exon:21830;Parent=Msa0179240-mRNA-1
chr1_4 exon 66661421 66661537 66661421 ID=Msa0179240-mRNA-1:exon:21831;Parent=Msa0179240-mRNA-1
chr1_4 exon 66661857 66662006 66661857 ID=Msa0179240-mRNA-1:exon:21832;Parent=Msa0179240-mRNA-1
chr1_4 exon 66662298 66664217 66662298 ID=Msa0179240-mRNA-1:exon:21833;Parent=Msa0179240-mRNA-1
chr1_4 CDS 66659797 66659878 66659797 ID=Msa0179240-mRNA-1:cds;Parent=Msa0179240-mRNA-1
chr1_4 CDS 66660171 66660409 66660171 ID=Msa0179240-mRNA-1:cds;Parent=Msa0179240-mRNA-1
chr1_4 CDS 66660533 66661342 66660533 ID=Msa0179240-mRNA-1:cds;Parent=Msa0179240-mRNA-1
chr1_4 CDS 66661421 66661537 66661421 ID=Msa0179240-mRNA-1:cds;Parent=Msa0179240-mRNA-1
chr1_4 CDS 66661857 66662006 66661857 ID=Msa0179240-mRNA-1:cds;Parent=Msa0179240-mRNA-1
chr1_4 CDS 66662298 66664217 66662298 ID=Msa0179240-mRNA-1:cds;Parent=Msa0179240-mRNA-1
Gene Sequence

>Msa0179240

ATGGATTACAACATTGACTTTGTGATATTGAAAAGATTGTCATACGATAATAGCGCTATAATGGATATTCATGGTGCACATACATTTGAAGCAGTATTGAATGGCTCATGGGAACCTGTGGAACTCATAAAAATTGATTCTGGGAAAACTACCATACATTTCCTCCATGCACAACAAACTCATAGTAATTTGTCATCAGACATCCGGATAAGATCAAGAAAAGCTACTTCGACTGATTGCTCCAGTTTCCTAAGACCTGGAATTGACATATCTGTGCTATCAAGCTTTCGATACGATAAAAACTCACGTCAATCTAGCCCAATGTCGACTGATGCTAGAGTGAATTCCATACACAAAAAACCTCACAAATATAACCAATGTTCATGTCGATTTCATGTAAACTTTTATGATGATCAAGGTTCACTTGGCACTGAAATAAAAACTCTGAAGGAAGAGGATAATGTGATTGGAATTGAGAAAATTTTCATCCTCCAAAGACTTCAACGAAATAACTGTGAAGGATCGTTAAGAGAAAATGATTGTTCTGAAGTTAATTCTTTTAGAAGGGATTCATCAGAAGACTGCTCTTCATTGCCACAATCTAGGTTACTTTTCGGGAAGTTTTTGTCTGATCTTTCCTGGTTTGTTACCGCGTCATCTTTAAAGAAGGTTGCATTTGTCATAAGATCGGTTCAAAACAAGCTTGTGCATCAAGTTATGGGAAATGATCCTAATATTAGTAGTCCTATGAATGTTTTGAACTTCAAATCAGAAAACGGATCACTTGAGCATGATTATGACGGAGAAGGTGCAATGCGGCCAATGCCAAGAAGCGAAATTGGAGGTCTACGACGCTCAAAACGTAGGACCATACAACTTGAACATTACGTAGATTGTAATGATGAAAAGTTGGAAGGTGGTGATAATGTTTCTTCGGGACATGTTAATAATTTCTGCACAAAGAAGCTTAAGAGAAGGTGGTTGTTTAAGGAGCATATGGATTTGATAGCTTTATGGGAAGCGATACAATCCAAAGAAGGTGTTCAAAAGAAAACGACAAACAGTATCGATGATCATGAAGAACAGGATCATAGAGGCAGTAGAACGTTAAATGCTGATACCTGCGAGGAAGAGAAAGAAAACGTATCGAAAAGCGGTGATGAGGTAGAAAATCCTGATGATTTGGCTGGCATATGGGAAGAAATGAATACAGCTTTGACATCAAGTTATCTTCTTGATGGAAATGAGGGTGCAAATGCTGATGAGGTTTTAGCTGACACAAATAAAGAATCCAAGGAAACATGTGAACATGATTATCGATTGGATGAACAAATTGGAATATACTGCCGTACATGCGGCTTTGTGAAAACTGAGATACGATACATTTCTGAACCCATTGTGACAAGGCTAAAATGGTACGAACAGAAGAAACAATGCAGTGAAGATACGGAACAAGTTGATGAAGATGATAATAATGATACGTTCTCAACTGATGCTACTGATCTTGGTGAACCTATATCCAAAGAAAATGATAGTGTTTGGGAATTGATTCCTGAACTCAAAGAAAACATGCATGCACACCAGAAAAAAGCTTTCGAGTTTCTTTGGAAAAACATTGCAGGGTCTATGGATCAATCACTCATGAAAGAGAAATCTGACACAAGTGGTGGCTGTGTTATATCTCATGCTCCTGGAGCTGGAAAAACCTTTCTCATCATCTCTTTTCTTGTAAGCTATTTGAAATTATTTCCACAGAAAAGACCATTAGTCCTAGCTCCAAAGACCACACTCTACACATGGCAAAAAGAGTTCGAAAAATGGAATATTCCCGTGCCGGTGTATCTTATCCACAGTTGTCGAAGCCAGATACATTCAACGACACCAAAATCAATGGTTCTGCCTGGAGTTCCAAATTCTAATGGTGTCAAGCATGATTTGGATTGTCTAGAAAAGATCAAAAGCTGGAATTCACACCCTAGTGTTCTTGTCATGGGTTATTCTTCATTTTTAGCCTTAATGAGAACAGAAGATAAGAAAAATTCACACAGAAAGCGCATGGCTAAAGCATTGAGGGAAAGTCCTGGACTCTTGATACTTGATGAGGGACACAATCCTAGAAGTACCACATCAAAGTTGAGGAAGTGCTTAATGGATCTACCAGCAGCATTGAGAATTTTACTTTCTGGCACTTTGTTTCAAAACAATTTTGGGGAATACTTCAACACTCTCTGTTTAGCCAGACCAAAATTTATTCACGAAGTTCTGGAAGAGTTAGATTCTAAGTACAGAAGAGGCAAACTAGAGGAGGAAGCGCCTCATTTACTAGAGGCACGCGCGAGAAAATTCTTCTTGGAGAACATTGAAAAGAAAATGAACTCAAATATTGATGCGGAAAAAATGAAGGGTGTAGATGTGCTTCGAAAAATCACAAATGGTTTTATAGATGTTTATGACAGTGCAAGTTCTTCTGATACTCTTCCTGGTTTGCAAATCTATACATTGCTCGTGAATGCATCCGATGAACAACACGAGATTGTGCAGAAACTGCAAAAGAAAATGGCGGGCTGTACCGGTTACTCTCTTGAGGTTGAGCTTTTGATAACACTCGGGTCAATACACCCATGGTTGATCAAAACAGCAGAAACATGTGCAGCTAAGTTTTTCTCCGGTGAAGAGTTGAAAAAACTAGACGAAAACAAATTTGCTTTGAGAAAAGGTTCCAAAGTAAGATTTGTTTTGAGCCTAATCTCACGTGTGATGAGCAAGGAAAAGGTTCTTATCTTCTGTCACAACCTTGCACCAGTGAGGTTTTTAATAGAGTTATTTGAGAATCATTTTCAGTGGAAAAATGGTAAAGAGATTCTGCAACTAACTGGTGAACAAGACTTCTTTGAAAGAACGAATGTGATAGATAAATTTGAAGATCGTTGTGGCGATTCAAAGATACTTCTTGCTTCAATCAATGCTTGTTCTGAAGGTATCAGTTTAACATCAGCTTCAAGAGTGATTTTCTTGGATTCTGAGTGGAATCCTTCAAAAACAAAGCAAGCTATTGCACGCGCTTTTCGACCTGGTCAAGAGAAAATGGTTTATGTATATCACTTGTTGATGACTGGATCCATGGAGGAAGATAAATATAGAAGAACTACTTGGAAAGAGTGGGTTTCTTGTATGATTTTCAGTGAGGAGCTTGTTGAAGATCCTTCCGAATGGCAAGCTGAGAAGATTGAAGATGAAATACTCAGGGAGATGGTTGAAGAGGACAAGTCTAAACTCATTCATATGATTATGAAGAATGAAAAGACTTCAACAAGCTAA
Protein sequence

>Msa0179240

MDYNIDFVILKRLSYDNSAIMDIHGAHTFEAVLNGSWEPVELIKIDSGKTTIHFLHAQQTHSNLSSDIRIRSRKATSTDCSSFLRPGIDISVLSSFRYDKNSRQSSPMSTDARVNSIHKKPHKYNQCSCRFHVNFYDDQGSLGTEIKTLKEEDNVIGIEKIFILQRLQRNNCEGSLRENDCSEVNSFRRDSSEDCSSLPQSRLLFGKFLSDLSWFVTASSLKKVAFVIRSVQNKLVHQVMGNDPNISSPMNVLNFKSENGSLEHDYDGEGAMRPMPRSEIGGLRRSKRRTIQLEHYVDCNDEKLEGGDNVSSGHVNNFCTKKLKRRWLFKEHMDLIALWEAIQSKEGVQKKTTNSIDDHEEQDHRGSRTLNADTCEEEKENVSKSGDEVENPDDLAGIWEEMNTALTSSYLLDGNEGANADEVLADTNKESKETCEHDYRLDEQIGIYCRTCGFVKTEIRYISEPIVTRLKWYEQKKQCSEDTEQVDEDDNNDTFSTDATDLGEPISKENDSVWELIPELKENMHAHQKKAFEFLWKNIAGSMDQSLMKEKSDTSGGCVISHAPGAGKTFLIISFLVSYLKLFPQKRPLVLAPKTTLYTWQKEFEKWNIPVPVYLIHSCRSQIHSTTPKSMVLPGVPNSNGVKHDLDCLEKIKSWNSHPSVLVMGYSSFLALMRTEDKKNSHRKRMAKALRESPGLLILDEGHNPRSTTSKLRKCLMDLPAALRILLSGTLFQNNFGEYFNTLCLARPKFIHEVLEELDSKYRRGKLEEEAPHLLEARARKFFLENIEKKMNSNIDAEKMKGVDVLRKITNGFIDVYDSASSSDTLPGLQIYTLLVNASDEQHEIVQKLQKKMAGCTGYSLEVELLITLGSIHPWLIKTAETCAAKFFSGEELKKLDENKFALRKGSKVRFVLSLISRVMSKEKVLIFCHNLAPVRFLIELFENHFQWKNGKEILQLTGEQDFFERTNVIDKFEDRCGDSKILLASINACSEGISLTSASRVIFLDSEWNPSKTKQAIARAFRPGQEKMVYVYHLLMTGSMEEDKYRRTTWKEWVSCMIFSEELVEDPSEWQAEKIEDEILREMVEEDKSKLIHMIMKNEKTSTS*