AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0180800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0180800 MtrunA17_Chr1g0204501 96.753 154 5 0 74 227 45 198 4.35e-107 306
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0180800 AT5G19910.1 68.790 157 32 4 74 227 51 193 1.99e-57 181
Msa0180800 AT5G19910.2 57.754 187 32 5 74 227 51 223 9.33e-51 165

Find 113 sgRNAs with CRISPR-Local

Find 227 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTAATAGAAGTATGATTT+TGG 0.136395 1_4:-68797640 Msa0180800:exon
GATATCACTATTTACCTTAT+TGG 0.153158 1_4:+68796218 None:intergenic
TGATCGACCTTTGTTATAAT+TGG 0.166073 1_4:-68797917 Msa0180800:five_prime_UTR
GTAGTGCATGCACAGGTGGC+TGG 0.230176 1_4:+68795669 None:intergenic
GTTTCGTCCATTTGTTTCTA+AGG 0.240006 1_4:-68798341 Msa0180800:five_prime_UTR
AGAGTTGAAGTAATAATAAT+AGG 0.256428 1_4:-68795459 Msa0180800:three_prime_UTR
GGATCCTTGTATATATTCTT+TGG 0.257913 1_4:+68797562 None:intergenic
TCAAGCCACCCTGCATAAAT+TGG 0.272984 1_4:-68797773 Msa0180800:five_prime_UTR
GAACCTAGTGCTGCGCTTCC+TGG 0.281659 1_4:-68795710 Msa0180800:CDS
CAGTTTGAAAATTTAATATA+TGG 0.284142 1_4:-68797882 Msa0180800:five_prime_UTR
AGAAAGTGAGTGCTTTATAT+TGG 0.289671 1_4:-68795512 Msa0180800:exon
ACTTAAAGTATCTTCAATAT+TGG 0.293258 1_4:-68796585 Msa0180800:CDS
TTTGTTATAATTGGCATATC+TGG 0.303686 1_4:-68797908 Msa0180800:five_prime_UTR
ATCTATTGTGCAGCACTTGT+TGG 0.309094 1_4:+68798174 None:intergenic
TATTTAAATGGTACAAGCTT+TGG 0.323173 1_4:-68795202 Msa0180800:three_prime_UTR
CATGCTTTGTCATTGCTGCT+TGG 0.324180 1_4:-68796723 Msa0180800:intron
TGTATCAAAGCTCCCAATCT+TGG 0.330312 1_4:+68798293 None:intergenic
ATGATAAATTTAATATATTC+AGG 0.332776 1_4:+68796556 None:intergenic
GGAGTATGTCTGTATTTAAA+TGG 0.347406 1_4:-68795214 Msa0180800:three_prime_UTR
AACCGTTGTCGCCCATCATC+TGG 0.352900 1_4:+68797541 None:intergenic
GTTTCTTATTCTCCTCGGAT+TGG 0.357717 1_4:-68797993 Msa0180800:five_prime_UTR
ATTAAAGGTGAGAGCTAAAC+AGG 0.361539 1_4:-68798236 Msa0180800:five_prime_UTR
GAATGGAAAACAAATAATAT+TGG 0.363161 1_4:+68797744 None:intergenic
AAATTAAAATCTGTAGTATA+CGG 0.364774 1_4:+68797689 None:intergenic
ATTTCATACTCTCCCAAGAT+TGG 0.365797 1_4:-68798306 Msa0180800:five_prime_UTR
CTAGGTTCTGGAAGTGGTCT+TGG 0.382881 1_4:+68795725 None:intergenic
TAGTGCATGCACAGGTGGCT+GGG 0.384524 1_4:+68795670 None:intergenic
TTTCTTATTCTCCTCGGATT+GGG 0.385548 1_4:-68797992 Msa0180800:five_prime_UTR
AATGGCACATCCTACCAATA+AGG 0.389767 1_4:-68796232 Msa0180800:intron
GGAAGCGCAGCACTAGGTTC+TGG 0.393441 1_4:+68795713 None:intergenic
TGTGCTACCCAACTTAAAAT+AGG 0.393795 1_4:+68795385 None:intergenic
AGAAGGGACATATGCCAATT+GGG 0.399848 1_4:+68798125 None:intergenic
CAGAAGGGACATATGCCAAT+TGG 0.400766 1_4:+68798124 None:intergenic
TTCTAGTTTCTTATTCTCCT+CGG 0.403178 1_4:-68797998 Msa0180800:five_prime_UTR
TCTACTTCTGGAAGAACTAT+AGG 0.406034 1_4:-68795772 Msa0180800:CDS
GAAATACTTCAGCTGATAGA+AGG 0.409099 1_4:-68795538 Msa0180800:CDS
TTATCGCTCCACTCCCCAAT+TGG 0.410613 1_4:-68798139 Msa0180800:five_prime_UTR
TTTGAGGACGAAGCATTCAT+TGG 0.411048 1_4:-68796610 Msa0180800:CDS
GCGCTTAAGTACAGCTCAAC+AGG 0.415512 1_4:+68797606 None:intergenic
GGCACACACTTCACGCTGAA+TGG 0.429818 1_4:+68797727 None:intergenic
GAGACGGATAAAACAGTTAG+AGG 0.430861 1_4:+68798093 None:intergenic
AAATTTGATAAAACAGAGAT+TGG 0.431232 1_4:-68798271 Msa0180800:five_prime_UTR
TGGTTATAACCAATTTATGC+AGG 0.437509 1_4:+68797764 None:intergenic
GTATCAAAGCTCCCAATCTT+GGG 0.440002 1_4:+68798294 None:intergenic
ATGCTTCGTCCTCAAAATAT+CGG 0.443322 1_4:+68796617 None:intergenic
TAGAAACAAATGGACGAAAC+AGG 0.452632 1_4:+68798344 None:intergenic
TAGTGCTGCGCTTCCTGGTC+CGG 0.453206 1_4:-68795705 Msa0180800:CDS
ATAGACAGCAGTTCTACTTC+TGG 0.455964 1_4:-68795784 Msa0180800:CDS
AGTGCATGCACAGGTGGCTG+GGG 0.456575 1_4:+68795671 None:intergenic
GGTTGAGTCGAGGCCGGACC+AGG 0.460454 1_4:+68795692 None:intergenic
TTTCATACTCTCCCAAGATT+GGG 0.461521 1_4:-68798305 Msa0180800:five_prime_UTR
GGCTGGGGTTGAGTCGAGGC+CGG 0.471060 1_4:+68795686 None:intergenic
TAAGGAGAGGCACTTTATGC+TGG 0.471146 1_4:-68797849 Msa0180800:five_prime_UTR
GAGTGTAAGCCAACCAGGCC+TGG 0.473699 1_4:-68797817 Msa0180800:five_prime_UTR
GAGTTGAAGTAATAATAATA+GGG 0.475472 1_4:-68795458 Msa0180800:three_prime_UTR
TCACTATTTACCTTATTGGT+AGG 0.479734 1_4:+68796222 None:intergenic
AGGGAAGCCTTAGAAACAAA+TGG 0.487757 1_4:+68798334 None:intergenic
TATACGGAAAGCCCTTGAAA+AGG 0.490993 1_4:+68797705 None:intergenic
TGATAAATTTAATATATTCA+GGG 0.495109 1_4:+68796557 None:intergenic
GAAGGATGAACGATGTATCA+AGG 0.498494 1_4:+68798047 None:intergenic
GGAAGAACTATAGGAACAAT+CGG 0.500395 1_4:-68795763 Msa0180800:CDS
GCAACGCTTGTAGCAGGTAC+TGG 0.502051 1_4:+68795644 None:intergenic
TTGAGGACGAAGCATTCATT+GGG 0.512149 1_4:-68796609 Msa0180800:CDS
TCTAACGAGTTTCAACCGTC+TGG 0.512281 1_4:+68795341 None:intergenic
GAAGAAGCTCAAGAAAATAG+AGG 0.520705 1_4:+68796277 None:intergenic
AAAATGAAATTGATCAAGAC+AGG 0.523673 1_4:+68797664 None:intergenic
ATATACAAGGATCCAGATGA+TGG 0.525951 1_4:-68797553 Msa0180800:CDS
AAGGTAATTGACAGGAAAAG+AGG 0.529830 1_4:+68798367 None:intergenic
AAAAGAGCAGACTGATCAAA+GGG 0.534798 1_4:+68797952 None:intergenic
GGTTATAACCAATTTATGCA+GGG 0.536876 1_4:+68797765 None:intergenic
GTTATGAGTGTAAGCCAACC+AGG 0.539396 1_4:-68797822 Msa0180800:five_prime_UTR
ACAGGAAAAGAGGCTAATGA+TGG 0.543975 1_4:+68798377 None:intergenic
AAGGATGAACGATGTATCAA+GGG 0.547169 1_4:+68798048 None:intergenic
GAGCTGTACTTAAGCGCTGA+TGG 0.548171 1_4:-68797600 Msa0180800:CDS
GGACCAGGAAGCGCAGCACT+AGG 0.549090 1_4:+68795707 None:intergenic
GTCACAGCAACGCTTGTAGC+AGG 0.549168 1_4:+68795638 None:intergenic
GCAGCACTAGGTTCTGGAAG+TGG 0.553833 1_4:+68795719 None:intergenic
ATACGGAAAGCCCTTGAAAA+GGG 0.555379 1_4:+68797706 None:intergenic
CAAAAGAGCAGACTGATCAA+AGG 0.567861 1_4:+68797951 None:intergenic
ATACCGTATGGCATTGGAGA+AGG 0.568373 1_4:+68795596 None:intergenic
GACATATGCCAATTGGGGAG+TGG 0.571738 1_4:+68798131 None:intergenic
GAAAACCATACAGTGGATGT+AGG 0.571854 1_4:+68797477 None:intergenic
GTAAGCCAACCAGGCCTGGT+TGG 0.572231 1_4:-68797813 Msa0180800:five_prime_UTR
AGGTGGCTGGGGTTGAGTCG+AGG 0.574599 1_4:+68795682 None:intergenic
GGGAGAGTATGAAATCTACA+AGG 0.578617 1_4:+68798314 None:intergenic
GAAACAGGAAGGTAATTGAC+AGG 0.583410 1_4:+68798359 None:intergenic
GTTAGAGGAGGAATGACAGA+AGG 0.586720 1_4:+68798108 None:intergenic
ACCATACAGTGGATGTAGGT+AGG 0.594759 1_4:+68797481 None:intergenic
ATCCAGATGATGGGCGACAA+CGG 0.595215 1_4:-68797543 Msa0180800:CDS
GCTCCTTCTCCAATGCCATA+CGG 0.595355 1_4:-68795599 Msa0180800:CDS
ACTTCAACTCTCATCTGCGA+TGG 0.598476 1_4:+68795471 None:intergenic
TTGATGTTACAAATGATGAA+AGG 0.600431 1_4:-68795235 Msa0180800:three_prime_UTR
GGTGGTAGTGCATGCACAGG+TGG 0.607758 1_4:+68795665 None:intergenic
TATACAAGGATCCAGATGAT+GGG 0.614053 1_4:-68797552 Msa0180800:CDS
AAACAGGAAAGGTGGAATGA+CGG 0.625544 1_4:-68798220 Msa0180800:five_prime_UTR
AGATATGCCAATTATAACAA+AGG 0.628476 1_4:+68797910 None:intergenic
ACGGATAAAACAGTTAGAGG+AGG 0.632952 1_4:+68798096 None:intergenic
TGAGAGCTAAACAGGAAAGG+TGG 0.639079 1_4:-68798228 Msa0180800:five_prime_UTR
AACAAATGGACGAAACAGGA+AGG 0.640927 1_4:+68798348 None:intergenic
TAGGCCAAAGAATATATACA+AGG 0.644719 1_4:-68797566 Msa0180800:CDS
GAAGGGACATATGCCAATTG+GGG 0.644738 1_4:+68798126 None:intergenic
TCAAGAAAATAGAGGCAGTG+AGG 0.646000 1_4:+68796285 None:intergenic
TTAGAGGAGGAATGACAGAA+GGG 0.646052 1_4:+68798109 None:intergenic
AGGTGAGAGCTAAACAGGAA+AGG 0.648812 1_4:-68798231 Msa0180800:five_prime_UTR
TATAACCAATTTATGCAGGG+TGG 0.649169 1_4:+68797768 None:intergenic
GGATGTAGGTAGGATTAGCA+AGG 0.649374 1_4:+68797491 None:intergenic
ATTGAAAATACATATCCAGA+CGG 0.649827 1_4:-68795356 Msa0180800:three_prime_UTR
ACTGGTGGTAGTGCATGCAC+AGG 0.653824 1_4:+68795662 None:intergenic
TGCTGTCTAAACCCAATCCG+AGG 0.660940 1_4:+68797981 None:intergenic
GGAGAGTATGAAATCTACAA+GGG 0.673313 1_4:+68798315 None:intergenic
AGGATGAACGATGTATCAAG+GGG 0.675967 1_4:+68798049 None:intergenic
ACGCTTGTAGCAGGTACTGG+TGG 0.714222 1_4:+68795647 None:intergenic
TTTGCACTACCGGAGCCAGG+GGG 0.746793 1_4:+68795572 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATAAATTTAATATATTCA+GGG + chr1_4:68797022-68797041 None:intergenic 10.0%
!! ATGATAAATTTAATATATTC+AGG + chr1_4:68797023-68797042 None:intergenic 10.0%
!!! AATTTCAAATTATCTTTTAA+TGG - chr1_4:68798290-68798309 Msa0180800:five_prime_UTR 10.0%
!! CAGTTTGAAAATTTAATATA+TGG - chr1_4:68795694-68795713 Msa0180800:CDS 15.0%
!! AAATTAAAATCTGTAGTATA+CGG + chr1_4:68795890-68795909 None:intergenic 15.0%
!!! ATTAATAGAAGTATGATTTT+GGG - chr1_4:68795937-68795956 Msa0180800:intron 15.0%
!!! TTTATTTAATTTCAAGTACA+TGG - chr1_4:68796321-68796340 Msa0180800:intron 15.0%
!!! GAAATAATTTTACAATTCAT+TGG + chr1_4:68798267-68798286 None:intergenic 15.0%
!! AAATTTGATAAAACAGAGAT+TGG - chr1_4:68795305-68795324 Msa0180800:three_prime_UTR 20.0%
!! CAAAAATAAAAAATCAGAGA+CGG + chr1_4:68795502-68795521 None:intergenic 20.0%
!! GAATGGAAAACAAATAATAT+TGG + chr1_4:68795835-68795854 None:intergenic 20.0%
!!! CATTAATAGAAGTATGATTT+TGG - chr1_4:68795936-68795955 Msa0180800:intron 20.0%
!! AATTGTTTAGTTAGAAAACA+AGG - chr1_4:68796210-68796229 Msa0180800:intron 20.0%
!! ATGTTGGAATTATAAGTAAT+CGG - chr1_4:68796251-68796270 Msa0180800:CDS 20.0%
!!! ATGTTTTTTTGTCAAAAAAG+GGG + chr1_4:68796831-68796850 None:intergenic 20.0%
!!! CATGTTTTTTTGTCAAAAAA+GGG + chr1_4:68796832-68796851 None:intergenic 20.0%
!!! ACTTAAAGTATCTTCAATAT+TGG - chr1_4:68796991-68797010 Msa0180800:intron 20.0%
!!! ATGTTTTACAATTCTACTTA+AGG - chr1_4:68797090-68797109 Msa0180800:intron 20.0%
!!! TGTTTTACAATTCTACTTAA+GGG - chr1_4:68797091-68797110 Msa0180800:intron 20.0%
!!! AGAAATCATGATTTTGTAAA+AGG + chr1_4:68797391-68797410 None:intergenic 20.0%
!!! ATTACTTGCATTTTAAGTTT+AGG - chr1_4:68797708-68797727 Msa0180800:five_prime_UTR 20.0%
!! AGAGTTGAAGTAATAATAAT+AGG - chr1_4:68798117-68798136 Msa0180800:five_prime_UTR 20.0%
!! GAGTTGAAGTAATAATAATA+GGG - chr1_4:68798118-68798137 Msa0180800:five_prime_UTR 20.0%
! TGGTAAGCCTAAAAAATTAA+AGG - chr1_4:68795325-68795344 Msa0180800:three_prime_UTR 25.0%
! AGATATGCCAATTATAACAA+AGG + chr1_4:68795669-68795688 None:intergenic 25.0%
!! TTTGTTATAATTGGCATATC+TGG - chr1_4:68795668-68795687 Msa0180800:CDS 25.0%
! ATATATGGTGCAAAAAGATA+AGG - chr1_4:68795709-68795728 Msa0180800:CDS 25.0%
! AAAATGAAATTGATCAAGAC+AGG + chr1_4:68795915-68795934 None:intergenic 25.0%
! ATAAAATGAAAACCATACAG+TGG + chr1_4:68796109-68796128 None:intergenic 25.0%
! AGATTATTCAAGAGTAGATT+AGG - chr1_4:68796289-68796308 Msa0180800:CDS 25.0%
! AGAAATTACAATGTGTTTCA+AGG + chr1_4:68796790-68796809 None:intergenic 25.0%
! ATTGTAATTTCTCTTAATCC+AGG - chr1_4:68796798-68796817 Msa0180800:intron 25.0%
!!! CCATGTTTTTTTGTCAAAAA+AGG + chr1_4:68796833-68796852 None:intergenic 25.0%
!!! CCTTTTTTGACAAAAAAACA+TGG - chr1_4:68796830-68796849 Msa0180800:intron 25.0%
! GCACTGAATTATGTTAAATA+GGG - chr1_4:68797113-68797132 Msa0180800:intron 25.0%
!! ACTCTATAAGAATTTTACAC+AGG - chr1_4:68797138-68797157 Msa0180800:intron 25.0%
!! CTCTATAAGAATTTTACACA+GGG - chr1_4:68797139-68797158 Msa0180800:intron 25.0%
! TAGAATAAAAATGACAGAAC+TGG + chr1_4:68797206-68797225 None:intergenic 25.0%
! GATATCACTATTTACCTTAT+TGG + chr1_4:68797361-68797380 None:intergenic 25.0%
! AGTACTATAATATGAAATCC+AGG + chr1_4:68797414-68797433 None:intergenic 25.0%
! AACTTAAAATGCAAGTAATC+TGG + chr1_4:68797707-68797726 None:intergenic 25.0%
!! TCTGGATATGTATTTTCAAT+TGG + chr1_4:68798220-68798239 None:intergenic 25.0%
! ATTGAAAATACATATCCAGA+CGG - chr1_4:68798220-68798239 Msa0180800:five_prime_UTR 25.0%
! TTGATGTTACAAATGATGAA+AGG - chr1_4:68798341-68798360 Msa0180800:five_prime_UTR 25.0%
! TATTTAAATGGTACAAGCTT+TGG - chr1_4:68798374-68798393 Msa0180800:five_prime_UTR 25.0%
!!! GCTCTCACCTTTAATTTTTT+AGG + chr1_4:68795335-68795354 None:intergenic 30.0%
!! TCTCAAAGTTTTGTACAAGA+AGG + chr1_4:68795550-68795569 None:intergenic 30.0%
!! TTCTAGTTTCTTATTCTCCT+CGG - chr1_4:68795578-68795597 Msa0180800:CDS 30.0%
ACAAAGGTCGATCATAAAAA+AGG + chr1_4:68795653-68795672 None:intergenic 30.0%
! TGATCGACCTTTGTTATAAT+TGG - chr1_4:68795659-68795678 Msa0180800:CDS 30.0%
GGTTATAACCAATTTATGCA+GGG + chr1_4:68795814-68795833 None:intergenic 30.0%
TGGTTATAACCAATTTATGC+AGG + chr1_4:68795815-68795834 None:intergenic 30.0%
!!! AGTATGATTTTGGGATTTGT+TGG - chr1_4:68795946-68795965 Msa0180800:intron 30.0%
TAGGCCAAAGAATATATACA+AGG - chr1_4:68796010-68796029 Msa0180800:intron 30.0%
GGATCCTTGTATATATTCTT+TGG + chr1_4:68796017-68796036 None:intergenic 30.0%
CAAGGAATAAGTTGTGAAAA+AGG - chr1_4:68796228-68796247 Msa0180800:intron 30.0%
TAAGTTGTGAAAAAGGATGT+TGG - chr1_4:68796235-68796254 Msa0180800:CDS 30.0%
TGAGAGAATATGATAGATGA+AGG + chr1_4:68796456-68796475 None:intergenic 30.0%
TTACAATGTGTTTCAAGGAA+AGG + chr1_4:68796785-68796804 None:intergenic 30.0%
! GTTACTCTTTTGAACAGATT+TGG - chr1_4:68796929-68796948 Msa0180800:intron 30.0%
! GGCACTGAATTATGTTAAAT+AGG - chr1_4:68797112-68797131 Msa0180800:intron 30.0%
!!! GCTCATTTTTTATGAATGCT+AGG - chr1_4:68797265-68797284 Msa0180800:intron 30.0%
TCACTATTTACCTTATTGGT+AGG + chr1_4:68797357-68797376 None:intergenic 30.0%
TACAAAATCATGATTTCTCC+TGG - chr1_4:68797393-68797412 Msa0180800:intron 30.0%
! TACAACAAAATTTGTTCTCG+AGG + chr1_4:68797477-68797496 None:intergenic 30.0%
ATGTAATGTCTGTCACTAAT+AGG - chr1_4:68797639-68797658 Msa0180800:exon 30.0%
! TAATAGGTTCTTTGTTTGCT+TGG - chr1_4:68797655-68797674 Msa0180800:five_prime_UTR 30.0%
ACCAAAAATTTCTCTGTTGT+AGG - chr1_4:68797760-68797779 Msa0180800:five_prime_UTR 30.0%
! TCCTACAACAGAGAAATTTT+TGG + chr1_4:68797764-68797783 None:intergenic 30.0%
AGAAAGTGAGTGCTTTATAT+TGG - chr1_4:68798064-68798083 Msa0180800:five_prime_UTR 30.0%
!! TTCTGTGACCTATTTTAAGT+TGG - chr1_4:68798183-68798202 Msa0180800:five_prime_UTR 30.0%
!!! TCTGTGACCTATTTTAAGTT+GGG - chr1_4:68798184-68798203 Msa0180800:five_prime_UTR 30.0%
GGAGTATGTCTGTATTTAAA+TGG - chr1_4:68798362-68798381 Msa0180800:five_prime_UTR 30.0%
AAGGTAATTGACAGGAAAAG+AGG + chr1_4:68795212-68795231 None:intergenic 35.0%
TAGAAACAAATGGACGAAAC+AGG + chr1_4:68795235-68795254 None:intergenic 35.0%
!! GTTTCGTCCATTTGTTTCTA+AGG - chr1_4:68795235-68795254 Msa0180800:three_prime_UTR 35.0%
! GGAGAGTATGAAATCTACAA+GGG + chr1_4:68795264-68795283 None:intergenic 35.0%
ATTTCATACTCTCCCAAGAT+TGG - chr1_4:68795270-68795289 Msa0180800:three_prime_UTR 35.0%
TTTCATACTCTCCCAAGATT+GGG - chr1_4:68795271-68795290 Msa0180800:three_prime_UTR 35.0%
ATTAAAGGTGAGAGCTAAAC+AGG - chr1_4:68795340-68795359 Msa0180800:three_prime_UTR 35.0%
AAGGATGAACGATGTATCAA+GGG + chr1_4:68795531-68795550 None:intergenic 35.0%
TTTCTTATTCTCCTCGGATT+GGG - chr1_4:68795584-68795603 Msa0180800:CDS 35.0%
AAAAGAGCAGACTGATCAAA+GGG + chr1_4:68795627-68795646 None:intergenic 35.0%
TATAACCAATTTATGCAGGG+TGG + chr1_4:68795811-68795830 None:intergenic 35.0%
ATATACAAGGATCCAGATGA+TGG - chr1_4:68796023-68796042 Msa0180800:intron 35.0%
TATACAAGGATCCAGATGAT+GGG - chr1_4:68796024-68796043 Msa0180800:intron 35.0%
TTAAGCAATAGCTGCATATG+TGG - chr1_4:68796156-68796175 Msa0180800:intron 35.0%
TGTCGAGTCATGTATTTGAA+AGG - chr1_4:68796180-68796199 Msa0180800:intron 35.0%
GTCGAGTCATGTATTTGAAA+GGG - chr1_4:68796181-68796200 Msa0180800:intron 35.0%
TCAAGAGTAGATTAGGTGTT+TGG - chr1_4:68796296-68796315 Msa0180800:intron 35.0%
CAAGTACATGGTCTGTTTAA+CGG - chr1_4:68796333-68796352 Msa0180800:intron 35.0%
AAGTACATGGTCTGTTTAAC+GGG - chr1_4:68796334-68796353 Msa0180800:intron 35.0%
GACATTGAGTTTAGTGACAT+TGG - chr1_4:68796369-68796388 Msa0180800:intron 35.0%
! ACATTGAGCTTTTGTGACAT+CGG - chr1_4:68796537-68796556 Msa0180800:intron 35.0%
! ACATTGAGCTTTTGTGACAT+CGG - chr1_4:68796537-68796556 Msa0180800:intron 35.0%
TCCGCTTAATAGATGTATTG+AGG - chr1_4:68796753-68796772 Msa0180800:CDS 35.0%
CCCTCAATACATCTATTAAG+CGG + chr1_4:68796757-68796776 None:intergenic 35.0%
CCGCTTAATAGATGTATTGA+GGG - chr1_4:68796754-68796773 Msa0180800:CDS 35.0%
ATGCTTCGTCCTCAAAATAT+CGG + chr1_4:68796962-68796981 None:intergenic 35.0%
! TAGCGATGCTTTGTTCAAAT+GGG - chr1_4:68797224-68797243 Msa0180800:intron 35.0%
GAAGAAGCTCAAGAAAATAG+AGG + chr1_4:68797302-68797321 None:intergenic 35.0%
! TGCAAATTTTCGCAATGCAA+TGG - chr1_4:68797326-68797345 Msa0180800:intron 35.0%
GAGACAAAGATAGACAGATA+TGG - chr1_4:68797510-68797529 Msa0180800:CDS 35.0%
TACCTACTGCATCACAATTT+CGG + chr1_4:68797576-68797595 None:intergenic 35.0%
TTTCTTTGCCTTGTGTTTCT+TGG - chr1_4:68797733-68797752 Msa0180800:five_prime_UTR 35.0%
! TCTACTTCTGGAAGAACTAT+AGG - chr1_4:68797804-68797823 Msa0180800:five_prime_UTR 35.0%
GGAAGAACTATAGGAACAAT+CGG - chr1_4:68797813-68797832 Msa0180800:five_prime_UTR 35.0%
!!! CATGTCATTTTTTGCACTAC+CGG + chr1_4:68798017-68798036 None:intergenic 35.0%
GAAATACTTCAGCTGATAGA+AGG - chr1_4:68798038-68798057 Msa0180800:five_prime_UTR 35.0%
TGTGCTACCCAACTTAAAAT+AGG + chr1_4:68798194-68798213 None:intergenic 35.0%
ACAGGAAAAGAGGCTAATGA+TGG + chr1_4:68795202-68795221 None:intergenic 40.0%
GAAACAGGAAGGTAATTGAC+AGG + chr1_4:68795220-68795239 None:intergenic 40.0%
AACAAATGGACGAAACAGGA+AGG + chr1_4:68795231-68795250 None:intergenic 40.0%
AGGGAAGCCTTAGAAACAAA+TGG + chr1_4:68795245-68795264 None:intergenic 40.0%
! GGGAGAGTATGAAATCTACA+AGG + chr1_4:68795265-68795284 None:intergenic 40.0%
GTATCAAAGCTCCCAATCTT+GGG + chr1_4:68795285-68795304 None:intergenic 40.0%
TGTATCAAAGCTCCCAATCT+TGG + chr1_4:68795286-68795305 None:intergenic 40.0%
AAACAGGAAAGGTGGAATGA+CGG - chr1_4:68795356-68795375 Msa0180800:three_prime_UTR 40.0%
ATCTATTGTGCAGCACTTGT+TGG + chr1_4:68795405-68795424 None:intergenic 40.0%
AGAAGGGACATATGCCAATT+GGG + chr1_4:68795454-68795473 None:intergenic 40.0%
TTAGAGGAGGAATGACAGAA+GGG + chr1_4:68795470-68795489 None:intergenic 40.0%
ACGGATAAAACAGTTAGAGG+AGG + chr1_4:68795483-68795502 None:intergenic 40.0%
GAGACGGATAAAACAGTTAG+AGG + chr1_4:68795486-68795505 None:intergenic 40.0%
AGGATGAACGATGTATCAAG+GGG + chr1_4:68795530-68795549 None:intergenic 40.0%
GAAGGATGAACGATGTATCA+AGG + chr1_4:68795532-68795551 None:intergenic 40.0%
GTTTCTTATTCTCCTCGGAT+TGG - chr1_4:68795583-68795602 Msa0180800:CDS 40.0%
CAAAAGAGCAGACTGATCAA+AGG + chr1_4:68795628-68795647 None:intergenic 40.0%
TGGTGCAAAAAGATAAGGAG+AGG - chr1_4:68795714-68795733 Msa0180800:CDS 40.0%
! CTTCCTTTGAAAAACCAACC+AGG + chr1_4:68795780-68795799 None:intergenic 40.0%
ATACGGAAAGCCCTTGAAAA+GGG + chr1_4:68795873-68795892 None:intergenic 40.0%
TATACGGAAAGCCCTTGAAA+AGG + chr1_4:68795874-68795893 None:intergenic 40.0%
!!! GCTGATGGTTGTTTTTCTCT+AGG - chr1_4:68795991-68796010 Msa0180800:intron 40.0%
GAAAACCATACAGTGGATGT+AGG + chr1_4:68796102-68796121 None:intergenic 40.0%
TAAAGTCACCGATGTCACAA+AGG - chr1_4:68796487-68796506 Msa0180800:intron 40.0%
ACATTGAGCCTTTGTGACAT+CGG + chr1_4:68796498-68796517 None:intergenic 40.0%
TCAATGTCACGGAAACTACT+TGG - chr1_4:68796511-68796530 Msa0180800:intron 40.0%
CAATGTCACGGAAACTACTT+GGG - chr1_4:68796512-68796531 Msa0180800:intron 40.0%
CAAAAAAGGGGAATTTCACC+TGG + chr1_4:68796819-68796838 None:intergenic 40.0%
! TTTGAGGACGAAGCATTCAT+TGG - chr1_4:68796966-68796985 Msa0180800:intron 40.0%
! TTGAGGACGAAGCATTCATT+GGG - chr1_4:68796967-68796986 Msa0180800:intron 40.0%
CTTATTGTCCCACTAAAGTC+TGG - chr1_4:68797057-68797076 Msa0180800:intron 40.0%
! CTAGCGATGCTTTGTTCAAA+TGG - chr1_4:68797223-68797242 Msa0180800:intron 40.0%
TCAAGAAAATAGAGGCAGTG+AGG + chr1_4:68797294-68797313 None:intergenic 40.0%
AATGGCACATCCTACCAATA+AGG - chr1_4:68797344-68797363 Msa0180800:intron 40.0%
TACCGAAATTGTGATGCAGT+AGG - chr1_4:68797571-68797590 Msa0180800:CDS 40.0%
TGGTCAATCCAAGAAACACA+AGG + chr1_4:68797744-68797763 None:intergenic 40.0%
ATAGACAGCAGTTCTACTTC+TGG - chr1_4:68797792-68797811 Msa0180800:five_prime_UTR 40.0%
AGGTGAGAGCTAAACAGGAA+AGG - chr1_4:68795345-68795364 Msa0180800:three_prime_UTR 45.0%
TGAGAGCTAAACAGGAAAGG+TGG - chr1_4:68795348-68795367 Msa0180800:three_prime_UTR 45.0%
GAAGGGACATATGCCAATTG+GGG + chr1_4:68795453-68795472 None:intergenic 45.0%
CAGAAGGGACATATGCCAAT+TGG + chr1_4:68795455-68795474 None:intergenic 45.0%
GTTAGAGGAGGAATGACAGA+AGG + chr1_4:68795471-68795490 None:intergenic 45.0%
!! TAAGGAGAGGCACTTTATGC+TGG - chr1_4:68795727-68795746 Msa0180800:CDS 45.0%
GTTATGAGTGTAAGCCAACC+AGG - chr1_4:68795754-68795773 Msa0180800:CDS 45.0%
TTTGAAAAACCAACCAGGCC+TGG + chr1_4:68795775-68795794 None:intergenic 45.0%
!!! AGGCCTGGTTGGTTTTTCAA+AGG - chr1_4:68795774-68795793 Msa0180800:CDS 45.0%
TCAAGCCACCCTGCATAAAT+TGG - chr1_4:68795803-68795822 Msa0180800:intron 45.0%
! TGAAGTGTGTGCCCTTTTCA+AGG - chr1_4:68795859-68795878 Msa0180800:intron 45.0%
! GAAGTGTGTGCCCTTTTCAA+GGG - chr1_4:68795860-68795879 Msa0180800:intron 45.0%
! ACAACGGTTTTTGCTGGAAC+TGG - chr1_4:68796049-68796068 Msa0180800:intron 45.0%
! GGATGTAGGTAGGATTAGCA+AGG + chr1_4:68796088-68796107 None:intergenic 45.0%
TCCTACCTACATCCACTGTA+TGG - chr1_4:68796094-68796113 Msa0180800:intron 45.0%
ACCATACAGTGGATGTAGGT+AGG + chr1_4:68796098-68796117 None:intergenic 45.0%
!! TCTGTTTAACGGGTTGCACT+TGG - chr1_4:68796344-68796363 Msa0180800:intron 45.0%
GTCACAAAGGCTCAATGTCA+CGG - chr1_4:68796500-68796519 Msa0180800:intron 45.0%
!!! AGCTTTTGTGACATCGGCTT+GGG - chr1_4:68796543-68796562 Msa0180800:intron 45.0%
TGTGACATCGGTGACTTTAG+TGG - chr1_4:68796580-68796599 Msa0180800:CDS 45.0%
GAGTTGCACTTGGATGACAT+TGG + chr1_4:68796688-68796707 None:intergenic 45.0%
GGAAACTACTTGGGTGACAT+TGG + chr1_4:68796521-68796540 None:intergenic 45.0%
ACCCAAGTAGTTTCCCTGTT+GGG - chr1_4:68796715-68796734 Msa0180800:intron 45.0%
ACCCAACAGGGAAACTACTT+GGG + chr1_4:68796719-68796738 None:intergenic 45.0%
AACCCAACAGGGAAACTACT+TGG + chr1_4:68796720-68796739 None:intergenic 45.0%
CATGCTTTGTCATTGCTGCT+TGG - chr1_4:68796853-68796872 Msa0180800:intron 45.0%
! GGCTCAGAACCGATATTTTG+AGG - chr1_4:68796950-68796969 Msa0180800:intron 45.0%
TCCCACTAAAGTCTGGAAGA+GGG - chr1_4:68797064-68797083 Msa0180800:intron 45.0%
TCCCTCTTCCAGACTTTAGT+GGG + chr1_4:68797068-68797087 None:intergenic 45.0%
ATCCCTCTTCCAGACTTTAG+TGG + chr1_4:68797069-68797088 None:intergenic 45.0%
! CGATGCTTTGTTCAAATGGG+TGG - chr1_4:68797227-68797246 Msa0180800:intron 45.0%
! ATACCGTATGGCATTGGAGA+AGG + chr1_4:68797983-68798002 None:intergenic 45.0%
ACTTCAACTCTCATCTGCGA+TGG + chr1_4:68798108-68798127 None:intergenic 45.0%
TCTAACGAGTTTCAACCGTC+TGG + chr1_4:68798238-68798257 None:intergenic 45.0%
TTATCGCTCCACTCCCCAAT+TGG - chr1_4:68795437-68795456 Msa0180800:three_prime_UTR 50.0%
GACATATGCCAATTGGGGAG+TGG + chr1_4:68795448-68795467 None:intergenic 50.0%
TGCTGTCTAAACCCAATCCG+AGG + chr1_4:68795598-68795617 None:intergenic 50.0%
AAAAACCAACCAGGCCTGGT+TGG + chr1_4:68795771-68795790 None:intergenic 50.0%
GCGCTTAAGTACAGCTCAAC+AGG + chr1_4:68795973-68795992 None:intergenic 50.0%
!! GAGCTGTACTTAAGCGCTGA+TGG - chr1_4:68795976-68795995 Msa0180800:intron 50.0%
!! ATCCAGATGATGGGCGACAA+CGG - chr1_4:68796033-68796052 Msa0180800:intron 50.0%
!! GAGCTTTTGTGACATCGGCT+TGG - chr1_4:68796542-68796561 Msa0180800:intron 50.0%
GGTGACATTGGAGTTGCACT+TGG + chr1_4:68796698-68796717 None:intergenic 50.0%
CACCCAAGTAGTTTCCCTGT+TGG - chr1_4:68796714-68796733 Msa0180800:intron 50.0%
GTCCCACTAAAGTCTGGAAG+AGG - chr1_4:68797063-68797082 Msa0180800:intron 50.0%
!! CTAGGTTCTGGAAGTGGTCT+TGG + chr1_4:68797854-68797873 None:intergenic 50.0%
GCTCCTTCTCCAATGCCATA+CGG - chr1_4:68797977-68797996 Msa0180800:five_prime_UTR 50.0%
! TTTTTGCACTACCGGAGCCA+GGG + chr1_4:68798009-68798028 None:intergenic 50.0%
!!! TTTTTTGCACTACCGGAGCC+AGG + chr1_4:68798010-68798029 None:intergenic 50.0%
GGCACACACTTCACGCTGAA+TGG + chr1_4:68795852-68795871 None:intergenic 55.0%
AACCGTTGTCGCCCATCATC+TGG + chr1_4:68796038-68796057 None:intergenic 55.0%
! TGGGCGACAACGGTTTTTGC+TGG - chr1_4:68796043-68796062 Msa0180800:intron 55.0%
CCTCTCAGTGCAACCCAACA+GGG + chr1_4:68796731-68796750 None:intergenic 55.0%
! CCTGTTGGGTTGCACTGAGA+GGG - chr1_4:68796729-68796748 Msa0180800:CDS 55.0%
!! GCAGCACTAGGTTCTGGAAG+TGG + chr1_4:68797860-68797879 None:intergenic 55.0%
TAGTGCATGCACAGGTGGCT+GGG + chr1_4:68797909-68797928 None:intergenic 55.0%
! ACTGGTGGTAGTGCATGCAC+AGG + chr1_4:68797917-68797936 None:intergenic 55.0%
!! ACGCTTGTAGCAGGTACTGG+TGG + chr1_4:68797932-68797951 None:intergenic 55.0%
GCAACGCTTGTAGCAGGTAC+TGG + chr1_4:68797935-68797954 None:intergenic 55.0%
GTCACAGCAACGCTTGTAGC+AGG + chr1_4:68797941-68797960 None:intergenic 55.0%
! GGGGGTATACCGTATGGCAT+TGG + chr1_4:68797989-68798008 None:intergenic 55.0%
ATGCCATACGGTATACCCCC+TGG - chr1_4:68797989-68798008 Msa0180800:five_prime_UTR 55.0%
! TTTTGCACTACCGGAGCCAG+GGG + chr1_4:68798008-68798027 None:intergenic 55.0%
GAGTGTAAGCCAACCAGGCC+TGG - chr1_4:68795759-68795778 Msa0180800:CDS 60.0%
GTAAGCCAACCAGGCCTGGT+TGG - chr1_4:68795763-68795782 Msa0180800:CDS 60.0%
! CCCTGTTGGGTTGCACTGAG+AGG - chr1_4:68796728-68796747 Msa0180800:CDS 60.0%
CCCTCTCAGTGCAACCCAAC+AGG + chr1_4:68796732-68796751 None:intergenic 60.0%
! GGAAGCGCAGCACTAGGTTC+TGG + chr1_4:68797866-68797885 None:intergenic 60.0%
GAACCTAGTGCTGCGCTTCC+TGG - chr1_4:68797866-68797885 Msa0180800:five_prime_UTR 60.0%
TAGTGCTGCGCTTCCTGGTC+CGG - chr1_4:68797871-68797890 Msa0180800:five_prime_UTR 60.0%
AGTGCATGCACAGGTGGCTG+GGG + chr1_4:68797908-68797927 None:intergenic 60.0%
GTAGTGCATGCACAGGTGGC+TGG + chr1_4:68797910-68797929 None:intergenic 60.0%
! GGTGGTAGTGCATGCACAGG+TGG + chr1_4:68797914-68797933 None:intergenic 60.0%
! GAGCCAGGGGGTATACCGTA+TGG + chr1_4:68797995-68798014 None:intergenic 60.0%
TACGGTATACCCCCTGGCTC+CGG - chr1_4:68797995-68798014 Msa0180800:five_prime_UTR 60.0%
TTTGCACTACCGGAGCCAGG+GGG + chr1_4:68798007-68798026 None:intergenic 60.0%
! GGACCAGGAAGCGCAGCACT+AGG + chr1_4:68797872-68797891 None:intergenic 65.0%
AGGTGGCTGGGGTTGAGTCG+AGG + chr1_4:68797897-68797916 None:intergenic 65.0%
GGTTGAGTCGAGGCCGGACC+AGG + chr1_4:68797887-68797906 None:intergenic 70.0%
GGCTGGGGTTGAGTCGAGGC+CGG + chr1_4:68797893-68797912 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 68795194 68798404 68795194 ID=Msa0180800;Name=Msa0180800
chr1_4 mRNA 68795194 68798404 68795194 ID=Msa0180800-mRNA-1;Parent=Msa0180800;Name=Msa0180800-mRNA-1;_AED=0.02;_eAED=0.02;_QI=755|0.5|0.8|0.8|1|1|5|332|227
chr1_4 exon 68795194 68795816 68795194 ID=Msa0180800-mRNA-1:exon:22813;Parent=Msa0180800-mRNA-1
chr1_4 exon 68796233 68796311 68796233 ID=Msa0180800-mRNA-1:exon:22812;Parent=Msa0180800-mRNA-1
chr1_4 exon 68796556 68796652 68796556 ID=Msa0180800-mRNA-1:exon:22811;Parent=Msa0180800-mRNA-1
chr1_4 exon 68796724 68796778 68796724 ID=Msa0180800-mRNA-1:exon:22810;Parent=Msa0180800-mRNA-1
chr1_4 exon 68797488 68798404 68797488 ID=Msa0180800-mRNA-1:exon:22809;Parent=Msa0180800-mRNA-1
chr1_4 five_prime_UTR 68797650 68798404 68797650 ID=Msa0180800-mRNA-1:five_prime_utr;Parent=Msa0180800-mRNA-1
chr1_4 CDS 68797488 68797649 68797488 ID=Msa0180800-mRNA-1:cds;Parent=Msa0180800-mRNA-1
chr1_4 CDS 68796724 68796778 68796724 ID=Msa0180800-mRNA-1:cds;Parent=Msa0180800-mRNA-1
chr1_4 CDS 68796556 68796652 68796556 ID=Msa0180800-mRNA-1:cds;Parent=Msa0180800-mRNA-1
chr1_4 CDS 68796233 68796311 68796233 ID=Msa0180800-mRNA-1:cds;Parent=Msa0180800-mRNA-1
chr1_4 CDS 68795526 68795816 68795526 ID=Msa0180800-mRNA-1:cds;Parent=Msa0180800-mRNA-1
chr1_4 three_prime_UTR 68795194 68795525 68795194 ID=Msa0180800-mRNA-1:three_prime_utr;Parent=Msa0180800-mRNA-1
Gene Sequence

>Msa0180800

ATGATTTTGGGATTTGTTGGACCTGTTGAGCTGTACTTAAGCGCTGATGGTTGTTTTTCTCTAGGCCAAAGAATATATACAAGGATCCAGATGATGGGCGACAACGGTTTTTGCTGGAACTGGAATTTGTTCAATGCCTTGCTAATCCTACCTACATCCACTGTGAAATTCCCCTTTTTTGACAAAAAAACATGGCATGCTTTGTCATTGCTGCTTGATTTGGCTCAGAACCGATATTTTGAGGACGAAGCATTCATTGGGTACTTAAAGTATCTTCAATATTGGCAACGCCCTGAATATATTAAATTTATCATGTATCCTCACTGCCTCTATTTTCTTGAGCTTCTTCAGAATGCAAATTTTCGCAATGCAATGGCACATCCTACCAATAAGGAATTGACACATAGACAGCAGTTCTACTTCTGGAAGAACTATAGGAACAATCGGCTAAAACACATTTTACCAAGACCACTTCCAGAACCTAGTGCTGCGCTTCCTGGTCCGGCCTCGACTCAACCCCAGCCACCTGTGCATGCACTACCACCAGTACCTGCTACAAGCGTTGCTGTGACAACATCGTCTAGTCAAGCTCCTTCTCCAATGCCATACGGTATACCCCCTGGCTCCGGTAGTGCAAAAAATGACATGAGAAATACTTCAGCTGATAGAAGGAAAAGAAAGTGA
Protein sequence

>Msa0180800

MILGFVGPVELYLSADGCFSLGQRIYTRIQMMGDNGFCWNWNLFNALLILPTSTVKFPFFDKKTWHALSLLLDLAQNRYFEDEAFIGYLKYLQYWQRPEYIKFIMYPHCLYFLELLQNANFRNAMAHPTNKELTHRQQFYFWKNYRNNRLKHILPRPLPEPSAALPGPASTQPQPPVHALPPVPATSVAVTTSSSQAPSPMPYGIPPGSGSAKNDMRNTSADRRKRK*