Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0182010 | G7INX6 | 81.967 | 122 | 22 | 0 | 1 | 122 | 1 | 122 | 3.83e-69 | 213 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0182010 | TF | B3 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0182010 | MtrunA17_Chr2g0281511 | 81.967 | 122 | 22 | 0 | 1 | 122 | 1 | 122 | 7.37e-73 | 213 |
| Msa0182010 | MtrunA17_Chr2g0309121 | 79.508 | 122 | 24 | 1 | 1 | 122 | 1 | 121 | 1.46e-68 | 202 |
| Msa0182010 | MtrunA17_Chr3g0081891 | 82.500 | 80 | 14 | 0 | 1 | 80 | 1 | 80 | 6.60e-43 | 135 |
| Msa0182010 | MtrunA17_Chr7g0276661 | 33.871 | 124 | 65 | 4 | 11 | 119 | 45 | 166 | 5.94e-19 | 77.4 |
| Msa0182010 | MtrunA17_Chr5g0430431 | 37.778 | 90 | 46 | 1 | 37 | 116 | 165 | 254 | 1.11e-15 | 70.5 |
| Msa0182010 | MtrunA17_Chr0c01g0489371 | 38.462 | 91 | 54 | 1 | 22 | 110 | 224 | 314 | 1.69e-14 | 68.2 |
| Msa0182010 | MtrunA17_Chr8g0372431 | 28.125 | 96 | 69 | 0 | 24 | 119 | 6 | 101 | 1.29e-13 | 62.0 |
| Msa0182010 | MtrunA17_Chr8g0356961 | 37.079 | 89 | 54 | 1 | 24 | 110 | 77 | 165 | 2.41e-13 | 63.2 |
| Msa0182010 | MtrunA17_Chr7g0228081 | 31.250 | 80 | 55 | 0 | 41 | 120 | 13 | 92 | 4.41e-12 | 57.8 |
| Msa0182010 | MtrunA17_Chr8g0356951 | 35.165 | 91 | 55 | 2 | 24 | 110 | 217 | 307 | 2.44e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAATGTTACTGCTTCTATTT+TGG | 0.172708 | 1_4:+70431946 | Msa0182010:CDS |
| AAAATGTTGATGTTCATTAT+TGG | 0.262235 | 1_4:+70431918 | Msa0182010:CDS |
| ATTCTCTAATTCAACACTAT+TGG | 0.312782 | 1_4:-70431891 | None:intergenic |
| TGTATGCTACTATCGTTAAC+TGG | 0.336033 | 1_4:+70432307 | Msa0182010:CDS |
| TTTGGTTGAAAATAATGTTT+TGG | 0.336817 | 1_4:+70431964 | Msa0182010:CDS |
| ATCACAGTCATAAGATTTAC+CGG | 0.342234 | 1_4:-70432157 | None:intergenic |
| CGATCAATAATCCTCCTAAT+AGG | 0.349986 | 1_4:+70432283 | Msa0182010:CDS |
| ATAGTAGCATACATCCTATT+AGG | 0.365679 | 1_4:-70432297 | None:intergenic |
| CTTCTTTCTGATGAAGAAAC+CGG | 0.407335 | 1_4:+70432138 | Msa0182010:CDS |
| CTTGAAAAGGGCCTAAAAGT+TGG | 0.503487 | 1_4:+70432243 | Msa0182010:CDS |
| CATCATAAATACATGGGAAG+AGG | 0.531704 | 1_4:+70432204 | Msa0182010:CDS |
| AAGCATGCATCATAAATACA+TGG | 0.533552 | 1_4:+70432197 | Msa0182010:CDS |
| CATTTCTCAGCAACACGTTG+AGG | 0.589472 | 1_4:-70432090 | None:intergenic |
| GTATGCTACTATCGTTAACT+GGG | 0.597139 | 1_4:+70432308 | Msa0182010:CDS |
| GTAGCATACATCCTATTAGG+AGG | 0.614063 | 1_4:-70432294 | None:intergenic |
| AGCATGCATCATAAATACAT+GGG | 0.618685 | 1_4:+70432198 | Msa0182010:CDS |
| ATAAATACATGGGAAGAGGA+TGG | 0.641504 | 1_4:+70432208 | Msa0182010:CDS |
| ATGATGCATGCTTCTTGACG+CGG | 0.660170 | 1_4:-70432187 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATGTTGATGTTCATTAT+TGG | + | chr1_4:70431918-70431937 | Msa0182010:CDS | 20.0% |
| !!! | TTTGGTTGAAAATAATGTTT+TGG | + | chr1_4:70431964-70431983 | Msa0182010:CDS | 20.0% |
| !!! | TACAATTTTGTTCTTGAAAA+GGG | + | chr1_4:70432231-70432250 | Msa0182010:CDS | 20.0% |
| ! | ATTCTCTAATTCAACACTAT+TGG | - | chr1_4:70431894-70431913 | None:intergenic | 25.0% |
| !! | TAATGTTACTGCTTCTATTT+TGG | + | chr1_4:70431946-70431965 | Msa0182010:CDS | 25.0% |
| !!! | GAAAATAATGTTTTGGTTAG+TGG | + | chr1_4:70431971-70431990 | Msa0182010:intron | 25.0% |
| !! | GTACAATTTTGTTCTTGAAA+AGG | + | chr1_4:70432230-70432249 | Msa0182010:CDS | 25.0% |
| !! | AAATAACATCACCAACTTTT+AGG | - | chr1_4:70432257-70432276 | None:intergenic | 25.0% |
| ATCACAGTCATAAGATTTAC+CGG | - | chr1_4:70432160-70432179 | None:intergenic | 30.0% | |
| AAGCATGCATCATAAATACA+TGG | + | chr1_4:70432197-70432216 | Msa0182010:CDS | 30.0% | |
| AGCATGCATCATAAATACAT+GGG | + | chr1_4:70432198-70432217 | Msa0182010:CDS | 30.0% | |
| ATAGTAGCATACATCCTATT+AGG | - | chr1_4:70432300-70432319 | None:intergenic | 30.0% | |
| CTTCTTTCTGATGAAGAAAC+CGG | + | chr1_4:70432138-70432157 | Msa0182010:CDS | 35.0% | |
| CATCATAAATACATGGGAAG+AGG | + | chr1_4:70432204-70432223 | Msa0182010:CDS | 35.0% | |
| ATAAATACATGGGAAGAGGA+TGG | + | chr1_4:70432208-70432227 | Msa0182010:CDS | 35.0% | |
| CGATCAATAATCCTCCTAAT+AGG | + | chr1_4:70432283-70432302 | Msa0182010:CDS | 35.0% | |
| TGTATGCTACTATCGTTAAC+TGG | + | chr1_4:70432307-70432326 | Msa0182010:CDS | 35.0% | |
| GTATGCTACTATCGTTAACT+GGG | + | chr1_4:70432308-70432327 | Msa0182010:CDS | 35.0% | |
| CTTGAAAAGGGCCTAAAAGT+TGG | + | chr1_4:70432243-70432262 | Msa0182010:CDS | 40.0% | |
| GTAGCATACATCCTATTAGG+AGG | - | chr1_4:70432297-70432316 | None:intergenic | 40.0% | |
| CATTTCTCAGCAACACGTTG+AGG | - | chr1_4:70432093-70432112 | None:intergenic | 45.0% | |
| ATGATGCATGCTTCTTGACG+CGG | - | chr1_4:70432190-70432209 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_4 | gene | 70431869 | 70432335 | 70431869 | ID=Msa0182010;Name=Msa0182010 |
| chr1_4 | mRNA | 70431869 | 70432335 | 70431869 | ID=Msa0182010-mRNA-1;Parent=Msa0182010;Name=Msa0182010-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|122 |
| chr1_4 | exon | 70431869 | 70431985 | 70431869 | ID=Msa0182010-mRNA-1:exon:23490;Parent=Msa0182010-mRNA-1 |
| chr1_4 | exon | 70432084 | 70432335 | 70432084 | ID=Msa0182010-mRNA-1:exon:23491;Parent=Msa0182010-mRNA-1 |
| chr1_4 | CDS | 70431869 | 70431985 | 70431869 | ID=Msa0182010-mRNA-1:cds;Parent=Msa0182010-mRNA-1 |
| chr1_4 | CDS | 70432084 | 70432335 | 70432084 | ID=Msa0182010-mRNA-1:cds;Parent=Msa0182010-mRNA-1 |
| Gene Sequence |
| Protein sequence |
