AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0188410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0188410 MtrunA17_Chr2g0277461 88.387 465 31 4 1 460 1 447 0.0 828
Msa0188410 MtrunA17_Chr2g0277531 72.152 316 72 6 7 317 14 318 9.91e-161 457
Msa0188410 MtrunA17_Chr1g0178011 49.891 459 198 10 6 455 5 440 1.81e-134 395
Msa0188410 MtrunA17_Chr5g0424931 45.665 519 204 13 7 458 13 520 7.77e-134 396
Msa0188410 MtrunA17_Chr5g0417281 44.015 518 218 13 3 458 1 508 2.26e-120 362
Msa0188410 MtrunA17_Chr5g0436761 43.376 468 239 13 5 459 4 458 1.10e-107 327
Msa0188410 MtrunA17_Chr5g0417211 42.765 463 191 12 5 459 4 400 4.69e-103 313
Msa0188410 MtrunA17_Chr2g0289221 52.516 318 136 6 6 317 13 321 7.92e-103 311
Msa0188410 MtrunA17_Chr3g0115271 35.572 551 248 16 5 458 11 551 2.59e-92 291
Msa0188410 MtrunA17_Chr5g0425261 45.198 354 164 13 7 346 6 343 2.50e-89 277
Msa0188410 MtrunA17_Chr5g0424801 45.042 353 164 13 7 344 6 343 3.10e-89 276
Msa0188410 MtrunA17_Chr5g0424741 41.388 418 176 15 6 363 5 413 8.87e-81 259
Msa0188410 MtrunA17_Chr2g0326761 39.152 401 179 12 12 358 10 399 5.22e-80 262
Msa0188410 MtrunA17_Chr4g0028971 36.740 411 191 11 4 355 3 403 3.85e-76 251
Msa0188410 MtrunA17_Chr1g0181211 35.075 402 191 11 12 353 26 417 5.12e-74 246
Msa0188410 MtrunA17_Chr1g0199681 38.035 397 186 13 13 358 94 481 2.42e-73 244
Msa0188410 MtrunA17_Chr7g0264171 35.507 414 191 15 10 358 28 430 1.85e-68 229
Msa0188410 MtrunA17_Chr7g0264161 39.617 313 158 12 23 317 2 301 1.64e-59 197
Msa0188410 MtrunA17_Chr5g0425661 49.268 205 93 4 13 214 37 233 2.66e-56 186
Msa0188410 MtrunA17_Chr8g0385791 32.398 392 247 9 13 391 59 445 8.50e-51 184
Msa0188410 MtrunA17_Chr8g0376771 33.711 353 212 9 13 349 23 369 2.83e-48 177
Msa0188410 MtrunA17_Chr4g0070181 33.333 354 209 10 10 348 20 361 2.97e-48 178
Msa0188410 MtrunA17_Chr2g0282961 30.747 348 205 9 13 337 53 387 1.46e-47 173
Msa0188410 MtrunA17_Chr2g0285461 32.670 352 218 8 13 349 23 370 2.07e-47 175
Msa0188410 MtrunA17_Chr2g0301251 32.768 354 211 10 10 348 20 361 4.19e-47 174
Msa0188410 MtrunA17_Chr3g0109391 31.842 380 239 8 13 379 26 398 4.51e-47 174
Msa0188410 MtrunA17_Chr5g0433301 32.295 353 218 8 13 349 24 371 4.74e-46 171
Msa0188410 MtrunA17_Chr2g0276781 32.500 360 218 9 13 356 23 373 2.64e-45 167
Msa0188410 MtrunA17_Chr1g0156321 33.529 340 207 9 13 338 43 377 3.39e-45 168
Msa0188410 MtrunA17_Chr4g0046371 32.044 362 196 11 8 337 33 376 5.16e-44 164
Msa0188410 MtrunA17_Chr8g0389081 32.479 351 217 10 5 340 24 369 3.63e-43 163
Msa0188410 MtrunA17_Chr4g0009751 30.055 366 237 9 13 365 19 378 1.36e-41 156
Msa0188410 MtrunA17_Chr8g0346711 31.065 338 212 9 13 337 29 358 8.47e-40 152
Msa0188410 MtrunA17_Chr7g0239061 28.647 377 243 9 13 373 14 380 1.11e-39 151
Msa0188410 MtrunA17_Chr4g0029671 30.435 368 228 10 13 356 54 417 1.14e-37 146
Msa0188410 MtrunA17_Chr2g0326281 30.278 360 212 8 13 337 56 411 1.36e-37 146
Msa0188410 MtrunA17_Chr5g0419861 58.333 120 40 6 51 165 9 123 7.14e-33 121
Msa0188410 MtrunA17_Chr5g0425651 48.780 123 51 6 239 350 1 122 1.66e-24 98.2
Msa0188410 MtrunA17_Chr5g0424791 41.830 153 71 5 66 214 13 151 1.14e-23 96.7
Msa0188410 MtrunA17_Chr8g0356211 27.928 222 136 8 131 337 8 220 4.47e-17 82.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0188410 AT1G77850.1 37.653 409 198 11 9 370 15 413 3.74e-85 273
Msa0188410 AT1G77850.2 37.653 409 198 11 9 370 15 413 3.82e-85 273
Msa0188410 AT4G30080.1 37.157 401 187 13 12 358 18 407 1.14e-73 244
Msa0188410 AT2G28350.2 35.749 414 195 12 5 358 2 404 1.51e-72 241
Msa0188410 AT2G28350.1 35.749 414 195 12 5 358 2 404 2.36e-72 242
Msa0188410 AT4G23980.1 33.731 335 208 6 13 337 12 342 1.03e-49 179
Msa0188410 AT4G23980.2 33.731 335 208 6 13 337 12 342 1.13e-49 179
Msa0188410 AT5G37020.2 31.806 371 238 7 13 370 22 390 2.57e-48 177
Msa0188410 AT5G37020.1 31.806 371 238 7 13 370 22 390 2.87e-48 177
Msa0188410 AT1G30330.1 33.428 353 214 9 13 349 23 370 4.27e-47 174
Msa0188410 AT1G30330.3 33.428 353 214 9 13 349 25 372 4.54e-47 174
Msa0188410 AT1G30330.2 33.428 353 214 9 13 349 25 372 4.54e-47 174
Msa0188410 AT5G62000.4 32.099 405 255 9 13 403 61 459 7.25e-47 174
Msa0188410 AT5G62000.2 34.167 360 215 10 13 356 61 414 7.79e-47 174
Msa0188410 AT5G62000.5 34.167 360 215 10 13 356 61 414 7.79e-47 174
Msa0188410 AT5G62000.3 34.167 360 215 10 13 356 61 414 7.79e-47 174
Msa0188410 AT5G62000.1 34.167 360 215 10 13 356 61 414 7.79e-47 174
Msa0188410 AT5G20730.1 32.687 361 217 9 3 348 14 363 1.05e-46 174
Msa0188410 AT5G20730.3 32.687 361 217 9 3 348 14 363 1.17e-46 174
Msa0188410 AT2G33860.1 31.988 347 202 9 13 337 54 388 1.45e-46 170
Msa0188410 AT2G46530.1 31.421 366 229 9 13 364 21 378 1.68e-46 170
Msa0188410 AT2G46530.3 31.421 366 229 9 13 364 42 399 3.43e-46 169
Msa0188410 AT5G20730.2 32.687 361 216 10 3 348 14 362 4.41e-46 172
Msa0188410 AT1G34310.1 33.237 346 200 9 8 337 22 352 2.95e-45 166
Msa0188410 AT3G61830.1 31.680 363 218 10 13 356 25 376 3.88e-45 166
Msa0188410 AT1G19850.2 34.294 347 202 11 10 338 40 378 2.16e-44 167
Msa0188410 AT1G19850.1 34.294 347 202 11 10 338 51 389 2.28e-44 167
Msa0188410 AT1G34390.1 32.659 346 200 10 8 337 22 350 5.59e-44 163
Msa0188410 AT1G35520.1 32.174 345 215 8 3 337 17 352 9.76e-44 162
Msa0188410 AT5G60450.2 31.445 353 212 8 8 337 36 381 1.54e-43 164
Msa0188410 AT1G19220.1 30.833 360 220 10 4 348 16 361 2.28e-43 164
Msa0188410 AT5G60450.1 31.609 348 208 8 13 337 65 405 4.06e-43 162
Msa0188410 AT1G35540.1 32.456 342 208 10 8 337 22 352 1.76e-42 159
Msa0188410 AT1G35240.1 32.353 340 211 8 8 337 22 352 2.76e-42 158
Msa0188410 AT1G34410.1 31.765 340 213 8 8 337 22 352 1.42e-41 156
Msa0188410 AT1G59750.2 31.339 351 222 9 13 350 22 366 2.08e-40 154
Msa0188410 AT1G59750.4 31.339 351 222 9 13 350 22 366 2.19e-40 154
Msa0188410 AT1G59750.1 31.339 351 222 9 13 350 22 366 2.30e-40 154
Msa0188410 AT1G59750.3 31.339 351 222 9 13 350 22 366 2.30e-40 154
Msa0188410 AT1G34170.1 30.372 349 222 9 1 337 13 352 4.42e-40 150
Msa0188410 AT1G34170.2 29.942 344 204 8 1 337 13 326 1.05e-37 144
Msa0188410 AT1G34170.3 29.942 344 204 8 1 337 13 326 3.86e-37 143
Msa0188410 AT3G61830.2 31.392 309 182 10 67 356 28 325 5.63e-32 129
Msa0188410 AT5G37020.3 32.584 267 165 7 117 370 31 295 3.13e-31 127
Msa0188410 AT1G43950.1 34.259 216 121 5 8 217 22 222 5.27e-30 116
Msa0188410 AT2G33860.2 32.479 234 141 6 117 337 2 231 7.88e-30 121
Msa0188410 AT2G46530.2 31.679 262 164 7 114 364 34 291 3.45e-29 120
Msa0188410 ATMG00940.1 43.210 81 42 3 236 313 65 144 9.99e-11 60.8

Find 101 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCTCGTACTTCTAATTTA+TGG 0.108193 2_1:-3600624 Msa0188410:CDS
TTTGAATATCGTAGGTTAAT+TGG 0.128409 2_1:-3599291 Msa0188410:intron
GATGGTTCAATTATGTTATT+TGG 0.174464 2_1:-3598932 Msa0188410:CDS
GCACATGTGTGCCAAATTTC+TGG 0.215696 2_1:+3601504 None:intergenic
CGCATGTGCGTCCAGAAATT+TGG 0.217939 2_1:-3601515 Msa0188410:CDS
TTCATCTTAACTCTCATTCT+AGG 0.234993 2_1:+3600694 None:intergenic
GATTGGCAACCTCTTCTTTA+GGG 0.275578 2_1:+3601238 None:intergenic
AGATTGGCAACCTCTTCTTT+AGG 0.275924 2_1:+3601237 None:intergenic
TAATTTATGGCGCATGCTTC+AGG 0.288610 2_1:-3600611 Msa0188410:intron
ATAGAGATTGTCCCTTGATA+TGG 0.294890 2_1:+3600649 None:intergenic
AGATGGACATCGTTGGTTAT+TGG 0.309930 2_1:+3601267 None:intergenic
TTGAATATCGTAGGTTAATT+GGG 0.314702 2_1:-3599290 Msa0188410:intron
TGCCCAACTAAGTGATAAAA+AGG 0.319098 2_1:-3599146 Msa0188410:CDS
CTGTTCGTTACCGACGTTCA+TGG 0.320691 2_1:-3601069 Msa0188410:CDS
TTCATCGCGGTTGTTGAGAT+TGG 0.361556 2_1:+3601221 None:intergenic
TAAACTCTTGAATGCAACTT+TGG 0.370692 2_1:+3601462 None:intergenic
GATATATAACATGTTTCTCT+TGG 0.384809 2_1:+3601008 None:intergenic
CAGAGGAGTTCAAAATAATA+TGG 0.402834 2_1:+3599002 None:intergenic
AATGGAATTCCTTTGTTAAG+AGG 0.404581 2_1:-3600981 Msa0188410:CDS
TCAACGGGAAAGTTATTTGT+TGG 0.427846 2_1:-3600916 Msa0188410:CDS
CAACGGGAAAGTTATTTGTT+GGG 0.431447 2_1:-3600915 Msa0188410:CDS
GCAGTTTGTTGCAGCGGCGG+CGG 0.440577 2_1:-3600878 Msa0188410:CDS
TGAGTTGGAGAAGGCAGTAA+TGG 0.441158 2_1:-3600842 Msa0188410:CDS
TCATCTTAACTCTCATTCTA+GGG 0.445548 2_1:+3600695 None:intergenic
ATGAATCAATGAAGATTCAA+TGG 0.450458 2_1:-3600720 Msa0188410:CDS
TGAGTCTTTCATGAAAATAA+CGG 0.452973 2_1:+3600936 None:intergenic
TGAAGACTGATAAATCTTCA+AGG 0.454632 2_1:-3600675 Msa0188410:CDS
CAGGTGTTGAGACGAGGATT+CGG 0.457588 2_1:+3601089 None:intergenic
TTGGGATGAATTTCAAGTCT+CGG 0.458166 2_1:-3599272 Msa0188410:CDS
ACTATCCTCGAGGGGATGAT+TGG 0.466368 2_1:-3600774 Msa0188410:CDS
AGTCAACCCGTGGTGGGTTG+AGG 0.468552 2_1:-3599236 Msa0188410:CDS
CACGCAGTTTGTTGCAGCGG+CGG 0.472211 2_1:-3600881 Msa0188410:CDS
CATGAACGTCGGTAACGAAC+AGG 0.475779 2_1:+3601070 None:intergenic
ATATATAACATGTTTCTCTT+GGG 0.481990 2_1:+3601009 None:intergenic
TAGAGATTGTCCCTTGATAT+GGG 0.482488 2_1:+3600650 None:intergenic
AAAAGAGTGTGTGTGGGTAA+TGG 0.489755 2_1:+3601389 None:intergenic
TCTCATATCAAGGGAGATGA+TGG 0.490070 2_1:-3598872 Msa0188410:CDS
CACCACGGGTTGACTCGTCT+TGG 0.505317 2_1:+3599244 None:intergenic
TGCCGATCCTCACACGGATG+AGG 0.509768 2_1:-3601313 Msa0188410:CDS
TCTTCAAGGATCCCATATCA+AGG 0.512818 2_1:-3600661 Msa0188410:CDS
AGTTATTTGTTGGGATTCGC+AGG 0.513561 2_1:-3600906 Msa0188410:CDS
AAGCCGCGGGAAGACATTAT+CGG 0.516617 2_1:+3601122 None:intergenic
AGTAATGGAGGCATTGAAGT+TGG 0.517015 2_1:-3600827 Msa0188410:CDS
GACCTCATCCGTGTGAGGAT+CGG 0.528604 2_1:+3601311 None:intergenic
TTTCACAATTCTCATATCAA+GGG 0.529587 2_1:-3598881 Msa0188410:CDS
ACATGTTATATATCTCTGAA+TGG 0.530162 2_1:-3600999 Msa0188410:CDS
AGCGGCGGCGGAGCAGAAGA+AGG 0.532222 2_1:-3600866 Msa0188410:CDS
AAGAGGAAGAAACTCGTGGC+TGG 0.538632 2_1:-3600964 Msa0188410:CDS
GGAGCAGAAGAAGGATGAGT+TGG 0.543711 2_1:-3600857 Msa0188410:CDS
GGTGATACTTTATCATGTAT+AGG 0.550061 2_1:+3599031 None:intergenic
TGGGAAACCCGCGGCAGACA+TGG 0.563883 2_1:+3601028 None:intergenic
AAATTGTTTACTATCCTCGA+GGG 0.568194 2_1:-3600783 Msa0188410:CDS
CGGCACAAAAGCGAGGTATG+TGG 0.571206 2_1:+3601142 None:intergenic
GAAGAAGGATGAGTTGGAGA+AGG 0.573545 2_1:-3600851 Msa0188410:CDS
AGAGTGTGTGTGGGTAATGG+AGG 0.575190 2_1:+3601392 None:intergenic
GAGACGAGGATTCGGCCTCT+AGG 0.578409 2_1:+3601097 None:intergenic
ATTTCTGGACGCACATGCGA+TGG 0.580120 2_1:+3601519 None:intergenic
TCTTGAAAACCCTAAAGAAG+AGG 0.581476 2_1:-3601247 Msa0188410:CDS
TCTCCTTGCCGATCCTCACA+CGG 0.588085 2_1:-3601319 Msa0188410:CDS
CTTCCCGCGGCTTGACCTAG+AGG 0.588102 2_1:-3601112 Msa0188410:CDS
AATGGAGGCATTGAAGTTGG+CGG 0.590740 2_1:-3600824 Msa0188410:CDS
AATGAGATCACTTCAACGGA+AGG 0.592309 2_1:-3599088 Msa0188410:CDS
GAAGTTGGCGGAAGAGAACA+AGG 0.600106 2_1:-3600812 Msa0188410:CDS
GTTCTGATGTTAACAATGCT+CGG 0.602948 2_1:-3601170 Msa0188410:CDS
AAATAACCTCAACCCACCAC+GGG 0.608942 2_1:+3599230 None:intergenic
CTTCAAGGATCCCATATCAA+GGG 0.609531 2_1:-3600660 Msa0188410:CDS
GAGACGATACAAGGAGTGAA+AGG 0.609567 2_1:+3601351 None:intergenic
TTTGTAGAAAAGAGTGTGTG+TGG 0.609647 2_1:+3601382 None:intergenic
GAACACGCAGTTTGTTGCAG+CGG 0.616426 2_1:-3600884 Msa0188410:CDS
GAGTTTCTTCCTCTTAACAA+AGG 0.617502 2_1:+3600972 None:intergenic
TTTGAATGAGATCACTTCAA+CGG 0.618869 2_1:-3599092 Msa0188410:CDS
GTGTTGCAATTGTGAGTCGA+AGG 0.619072 2_1:+3598968 None:intergenic
CGGCCTCTAGGTCAAGCCGC+GGG 0.620797 2_1:+3601109 None:intergenic
CGAAGGAAACTTTGTCTCAG+AGG 0.621557 2_1:+3598985 None:intergenic
TCAACAGCTGAGACGATACA+AGG 0.622390 2_1:+3601342 None:intergenic
GAAATTGTTTACTATCCTCG+AGG 0.624214 2_1:-3600784 Msa0188410:CDS
GTTGGAGAAGGCAGTAATGG+AGG 0.624776 2_1:-3600839 Msa0188410:CDS
ATGAGATCACTTCAACGGAA+GGG 0.630141 2_1:-3599087 Msa0188410:CDS
GTAATGGAGGAAGAAGAAGA+AGG 0.639327 2_1:+3601405 None:intergenic
AGAGTTTATTATTTCCCACA+AGG 0.642714 2_1:-3601447 Msa0188410:CDS
AACGAACAGGTGTTGAGACG+AGG 0.644655 2_1:+3601083 None:intergenic
CAAGACGAGTCAACCCGTGG+TGG 0.644679 2_1:-3599243 Msa0188410:CDS
TTGTGAAAATTGCTTACATG+AGG 0.646619 2_1:+3598896 None:intergenic
AAGACGAGTCAACCCGTGGT+GGG 0.646955 2_1:-3599242 Msa0188410:CDS
TCGGCCTCTAGGTCAAGCCG+CGG 0.648171 2_1:+3601108 None:intergenic
GAAATAACCTCAACCCACCA+CGG 0.649182 2_1:+3599229 None:intergenic
TGGTTGCTTCTGAACAGACA+TGG 0.659994 2_1:+3601539 None:intergenic
TGTTTCTCTTGGGAAACCCG+CGG 0.663878 2_1:+3601019 None:intergenic
ACAAAGACCTCATCCGTGTG+AGG 0.664295 2_1:+3601306 None:intergenic
TGTTAAGAGGAAGAAACTCG+TGG 0.667790 2_1:-3600968 Msa0188410:CDS
TTCCAAGACGAGTCAACCCG+TGG 0.669719 2_1:-3599246 Msa0188410:CDS
TTCAAAATAATATGGCTGTG+AGG 0.673161 2_1:+3599010 None:intergenic
ACATTATCGGCACAAAAGCG+AGG 0.676914 2_1:+3601135 None:intergenic
TTGTAGAAAAGAGTGTGTGT+GGG 0.681947 2_1:+3601383 None:intergenic
GTGCCGATAATGTCTTCCCG+CGG 0.682542 2_1:-3601125 Msa0188410:CDS
TGAAACTTCACCATGAACGT+CGG 0.683050 2_1:+3601059 None:intergenic
AATTGTTTACTATCCTCGAG+GGG 0.689459 2_1:-3600782 Msa0188410:CDS
TGATGGCTATATAAGAGCTG+CGG 0.708137 2_1:-3598855 None:intergenic
CATCCGTGTGAGGATCGGCA+AGG 0.710355 2_1:+3601316 None:intergenic
TCACACCAATCATCCCCTCG+AGG 0.748749 2_1:+3600769 None:intergenic
CAAAAGAAACAACTTCATCG+CGG 0.749390 2_1:+3601208 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATTTTCTTAAATTTATTAT+CGG + chr2_1:3599929-3599948 None:intergenic 10.0%
!!! ATTTTTTTGTGGTTTTTTTT+TGG - chr2_1:3600072-3600091 Msa0188410:intron 15.0%
!! CGTATATTTGAATATTTAAT+TGG - chr2_1:3600424-3600443 Msa0188410:intron 15.0%
!! GTAAAAAAAAATAGATGTTA+AGG + chr2_1:3600471-3600490 None:intergenic 15.0%
!!! CTTTATTTGAACAATTAATA+TGG - chr2_1:3600986-3601005 Msa0188410:CDS 15.0%
!! ACGATATTCAAATAAAAAAA+TGG + chr2_1:3601094-3601113 None:intergenic 15.0%
!!! TTTTTTTATTTGAATATCGT+AGG - chr2_1:3601094-3601113 Msa0188410:CDS 15.0%
!!! AGTTCTAAATTTTTTTGTTT+GGG + chr2_1:3601216-3601235 None:intergenic 15.0%
!!! TAGTTCTAAATTTTTTTGTT+TGG + chr2_1:3601217-3601236 None:intergenic 15.0%
!! ATATATAACATGTTTCTCTT+GGG + chr2_1:3599387-3599406 None:intergenic 20.0%
!!! ATAATTTCTTTTCCTTTTCT+TGG - chr2_1:3599806-3599825 Msa0188410:intron 20.0%
!!! GTTGATAAATGATTTTTTTG+TGG - chr2_1:3600061-3600080 Msa0188410:intron 20.0%
!!! GTTTTTTTTTGGTATAAATC+AGG - chr2_1:3600083-3600102 Msa0188410:intron 20.0%
!! TCTAATTATAGATATAGCAT+TGG + chr2_1:3600157-3600176 None:intergenic 20.0%
!! TTTAACTATAGATGAAACTT+TGG + chr2_1:3600873-3600892 None:intergenic 20.0%
!! ATAGTTAAATGACTCATTAA+AGG - chr2_1:3600884-3600903 Msa0188410:CDS 20.0%
!!! TTCTAAATTTTTTTGTTTGG+GGG + chr2_1:3601214-3601233 None:intergenic 20.0%
!!! GTTCTAAATTTTTTTGTTTG+GGG + chr2_1:3601215-3601234 None:intergenic 20.0%
! GATATATAACATGTTTCTCT+TGG + chr2_1:3599388-3599407 None:intergenic 25.0%
! ACATGTTATATATCTCTGAA+TGG - chr2_1:3599394-3599413 Msa0188410:intron 25.0%
! TGAGTCTTTCATGAAAATAA+CGG + chr2_1:3599460-3599479 None:intergenic 25.0%
!! TATTTTCATGAAAGACTCAA+CGG - chr2_1:3599461-3599480 Msa0188410:intron 25.0%
! ATGAATCAATGAAGATTCAA+TGG - chr2_1:3599673-3599692 Msa0188410:intron 25.0%
! TATCTCGTACTTCTAATTTA+TGG - chr2_1:3599769-3599788 Msa0188410:intron 25.0%
! AAATGAAATGCAATATCGAT+TGG + chr2_1:3600368-3600387 None:intergenic 25.0%
!! TCGGTTAATTTACCAATTTT+GGG - chr2_1:3600756-3600775 Msa0188410:CDS 25.0%
!! GTCATATGAGATAGATTTTA+AGG - chr2_1:3600917-3600936 Msa0188410:CDS 25.0%
!!! ATGAGATAGATTTTAAGGTT+AGG - chr2_1:3600922-3600941 Msa0188410:CDS 25.0%
!!! TGAGATAGATTTTAAGGTTA+GGG - chr2_1:3600923-3600942 Msa0188410:CDS 25.0%
! TTTGAATATCGTAGGTTAAT+TGG - chr2_1:3601102-3601121 Msa0188410:CDS 25.0%
! TTGAATATCGTAGGTTAATT+GGG - chr2_1:3601103-3601122 Msa0188410:CDS 25.0%
!! TTCCTTTTTATCACTTAGTT+GGG + chr2_1:3601252-3601271 None:intergenic 25.0%
!! TTTCCTTTTTATCACTTAGT+TGG + chr2_1:3601253-3601272 None:intergenic 25.0%
! AACACCAAAAATTATAGTGA+TGG - chr2_1:3601443-3601462 Msa0188410:CDS 25.0%
!! TGAACCATCACTATAATTTT+TGG + chr2_1:3601450-3601469 None:intergenic 25.0%
! GATGGTTCAATTATGTTATT+TGG - chr2_1:3601461-3601480 Msa0188410:CDS 25.0%
!! TTTTCACAATTCTCATATCA+AGG - chr2_1:3601511-3601530 Msa0188410:CDS 25.0%
! TTTCACAATTCTCATATCAA+GGG - chr2_1:3601512-3601531 Msa0188410:CDS 25.0%
!!! ATGCAACTTTGGAATTTTGA+CGG + chr2_1:3598923-3598942 None:intergenic 30.0%
TAAACTCTTGAATGCAACTT+TGG + chr2_1:3598934-3598953 None:intergenic 30.0%
AGAGTTTATTATTTCCCACA+AGG - chr2_1:3598946-3598965 Msa0188410:CDS 30.0%
AATGGAATTCCTTTGTTAAG+AGG - chr2_1:3599412-3599431 Msa0188410:intron 30.0%
! ATTTTCATGAAAGACTCAAC+GGG - chr2_1:3599462-3599481 Msa0188410:intron 30.0%
AAATTGTTTACTATCCTCGA+GGG - chr2_1:3599610-3599629 Msa0188410:intron 30.0%
TCATCTTAACTCTCATTCTA+GGG + chr2_1:3599701-3599720 None:intergenic 30.0%
TTCATCTTAACTCTCATTCT+AGG + chr2_1:3599702-3599721 None:intergenic 30.0%
TGAAGACTGATAAATCTTCA+AGG - chr2_1:3599718-3599737 Msa0188410:intron 30.0%
TTTGCATAAGATCCAAGAAA+AGG + chr2_1:3599821-3599840 None:intergenic 30.0%
GTGTTTCATAGCTAGATAAT+TGG + chr2_1:3599854-3599873 None:intergenic 30.0%
TATTAAACGTCTCCTAATGT+CGG + chr2_1:3600001-3600020 None:intergenic 30.0%
ATACTAGATACTATGAAGCA+CGG + chr2_1:3600032-3600051 None:intergenic 30.0%
CTGTAATAATCTCTCTATGT+GGG + chr2_1:3600543-3600562 None:intergenic 30.0%
TCTGTAATAATCTCTCTATG+TGG + chr2_1:3600544-3600563 None:intergenic 30.0%
!! AAAACAAACGAGGTTTTATG+AGG + chr2_1:3600573-3600592 None:intergenic 30.0%
! TCATAAAACCTCGTTTGTTT+TGG - chr2_1:3600572-3600591 Msa0188410:intron 30.0%
!! AATAAGCATTGATGTCAAAC+GGG + chr2_1:3600613-3600632 None:intergenic 30.0%
!! CAATAAGCATTGATGTCAAA+CGG + chr2_1:3600614-3600633 None:intergenic 30.0%
GTTTGACATCAATGCTTATT+GGG - chr2_1:3600613-3600632 Msa0188410:CDS 30.0%
ACATTGAGACTTATCCTATA+GGG + chr2_1:3600703-3600722 None:intergenic 30.0%
TACATTGAGACTTATCCTAT+AGG + chr2_1:3600704-3600723 None:intergenic 30.0%
! GTCGGTTAATTTACCAATTT+TGG - chr2_1:3600755-3600774 Msa0188410:CDS 30.0%
TAAATAGCAACTCCCAAAAT+TGG + chr2_1:3600771-3600790 None:intergenic 30.0%
ACAATTAATATGGTGCATTG+TGG - chr2_1:3600996-3601015 Msa0188410:CDS 30.0%
!!! AATTTTTTTGTTTGGGGGAA+TGG + chr2_1:3601209-3601228 None:intergenic 30.0%
TTTGAATGAGATCACTTCAA+CGG - chr2_1:3601301-3601320 Msa0188410:CDS 30.0%
GGTGATACTTTATCATGTAT+AGG + chr2_1:3601365-3601384 None:intergenic 30.0%
TTCAAAATAATATGGCTGTG+AGG + chr2_1:3601386-3601405 None:intergenic 30.0%
CAGAGGAGTTCAAAATAATA+TGG + chr2_1:3601394-3601413 None:intergenic 30.0%
TTGTGAAAATTGCTTACATG+AGG + chr2_1:3601500-3601519 None:intergenic 30.0%
! TTGTAGAAAAGAGTGTGTGT+GGG + chr2_1:3599013-3599032 None:intergenic 35.0%
! TTTGTAGAAAAGAGTGTGTG+TGG + chr2_1:3599014-3599033 None:intergenic 35.0%
!!! CTTCTTTAGGGTTTTCAAGA+TGG + chr2_1:3599146-3599165 None:intergenic 35.0%
TCTTGAAAACCCTAAAGAAG+AGG - chr2_1:3599146-3599165 Msa0188410:CDS 35.0%
CAAAAGAAACAACTTCATCG+CGG + chr2_1:3599188-3599207 None:intergenic 35.0%
GTTCTGATGTTAACAATGCT+CGG - chr2_1:3599223-3599242 Msa0188410:CDS 35.0%
GAGTTTCTTCCTCTTAACAA+AGG + chr2_1:3599424-3599443 None:intergenic 35.0%
TCAACGGGAAAGTTATTTGT+TGG - chr2_1:3599477-3599496 Msa0188410:intron 35.0%
CAACGGGAAAGTTATTTGTT+GGG - chr2_1:3599478-3599497 Msa0188410:intron 35.0%
GAAATTGTTTACTATCCTCG+AGG - chr2_1:3599609-3599628 Msa0188410:intron 35.0%
AATTGTTTACTATCCTCGAG+GGG - chr2_1:3599611-3599630 Msa0188410:intron 35.0%
! TGATTGGTGTGATTTTGTAG+TGG - chr2_1:3599635-3599654 Msa0188410:intron 35.0%
TAGAGATTGTCCCTTGATAT+GGG + chr2_1:3599746-3599765 None:intergenic 35.0%
ATAGAGATTGTCCCTTGATA+TGG + chr2_1:3599747-3599766 None:intergenic 35.0%
TTCAGTAGAATCGTATGTGT+CGG - chr2_1:3599955-3599974 Msa0188410:intron 35.0%
! TGCAATATCGATTGGTGTAA+TGG + chr2_1:3600360-3600379 None:intergenic 35.0%
AAATAGATGTTAAGGAGCGA+CGG + chr2_1:3600463-3600482 None:intergenic 35.0%
! TCAAACGGGTATTTTCATTG+TGG + chr2_1:3600599-3600618 None:intergenic 35.0%
CGTTTGACATCAATGCTTAT+TGG - chr2_1:3600612-3600631 Msa0188410:CDS 35.0%
GAGAACAAGTATGTTACTGT+CGG - chr2_1:3600737-3600756 Msa0188410:CDS 35.0%
! TTGGGATGAATTTCAAGTCT+CGG - chr2_1:3601121-3601140 Msa0188410:CDS 35.0%
TGCCCAACTAAGTGATAAAA+AGG - chr2_1:3601247-3601266 Msa0188410:CDS 35.0%
!!! AAGAAGGAGAAGCGTTTTCT+AGG + chr2_1:3598975-3598994 None:intergenic 40.0%
! GTAATGGAGGAAGAAGAAGA+AGG + chr2_1:3598991-3599010 None:intergenic 40.0%
! AAAAGAGTGTGTGTGGGTAA+TGG + chr2_1:3599007-3599026 None:intergenic 40.0%
! AGATGGACATCGTTGGTTAT+TGG + chr2_1:3599129-3599148 None:intergenic 40.0%
!! GTTTTCAAGATGGACATCGT+TGG + chr2_1:3599136-3599155 None:intergenic 40.0%
GATTGGCAACCTCTTCTTTA+GGG + chr2_1:3599158-3599177 None:intergenic 40.0%
AGATTGGCAACCTCTTCTTT+AGG + chr2_1:3599159-3599178 None:intergenic 40.0%
TGAAACTTCACCATGAACGT+CGG + chr2_1:3599337-3599356 None:intergenic 40.0%
TGTTAAGAGGAAGAAACTCG+TGG - chr2_1:3599425-3599444 Msa0188410:intron 40.0%
! AGTTATTTGTTGGGATTCGC+AGG - chr2_1:3599487-3599506 Msa0188410:intron 40.0%
!! AGTAATGGAGGCATTGAAGT+TGG - chr2_1:3599566-3599585 Msa0188410:intron 40.0%
!! TGGTGTGATTTTGTAGTGGA+TGG - chr2_1:3599639-3599658 Msa0188410:intron 40.0%
TCTTCAAGGATCCCATATCA+AGG - chr2_1:3599732-3599751 Msa0188410:intron 40.0%
CTTCAAGGATCCCATATCAA+GGG - chr2_1:3599733-3599752 Msa0188410:intron 40.0%
TAATTTATGGCGCATGCTTC+AGG - chr2_1:3599782-3599801 Msa0188410:intron 40.0%
AACACACTCACAAACACACA+CGG - chr2_1:3599869-3599888 Msa0188410:intron 40.0%
ACATCCTCACTCTTGCAAAA+AGG + chr2_1:3600300-3600319 None:intergenic 40.0%
! TTTTTGCAAGAGTGAGGATG+TGG - chr2_1:3600299-3600318 Msa0188410:intron 40.0%
!! CAAACGAGGTTTTATGAGGA+AGG + chr2_1:3600569-3600588 None:intergenic 40.0%
ATTGTGGACCAAAACAAACG+AGG + chr2_1:3600583-3600602 None:intergenic 40.0%
TATATTGCTTCGTGCCCTAT+AGG - chr2_1:3600686-3600705 Msa0188410:CDS 40.0%
ATATGGTGCATTGTGGCTAA+TGG - chr2_1:3601003-3601022 Msa0188410:CDS 40.0%
! TGCATTGTGGCTAATGGTTT+TGG - chr2_1:3601009-3601028 Msa0188410:CDS 40.0%
! GCATTGTGGCTAATGGTTTT+GGG - chr2_1:3601010-3601029 Msa0188410:CDS 40.0%
AATGAGATCACTTCAACGGA+AGG - chr2_1:3601305-3601324 Msa0188410:CDS 40.0%
ATGAGATCACTTCAACGGAA+GGG - chr2_1:3601306-3601325 Msa0188410:CDS 40.0%
TCTCATATCAAGGGAGATGA+TGG - chr2_1:3601521-3601540 Msa0188410:CDS 40.0%
GCACATGTGTGCCAAATTTC+TGG + chr2_1:3598892-3598911 None:intergenic 45.0%
!!! CTTTGGAATTTTGACGGCAG+CGG + chr2_1:3598917-3598936 None:intergenic 45.0%
! CGTTTTCTAGGTGACCTTGT+GGG + chr2_1:3598963-3598982 None:intergenic 45.0%
GAGACGATACAAGGAGTGAA+AGG + chr2_1:3599045-3599064 None:intergenic 45.0%
TCAACAGCTGAGACGATACA+AGG + chr2_1:3599054-3599073 None:intergenic 45.0%
TTCATCGCGGTTGTTGAGAT+TGG + chr2_1:3599175-3599194 None:intergenic 45.0%
ACATTATCGGCACAAAAGCG+AGG + chr2_1:3599261-3599280 None:intergenic 45.0%
! AAGTTTTACCATGTCTGCCG+CGG - chr2_1:3599357-3599376 Msa0188410:intron 45.0%
! GAAGAAGGATGAGTTGGAGA+AGG - chr2_1:3599542-3599561 Msa0188410:intron 45.0%
! TGAGTTGGAGAAGGCAGTAA+TGG - chr2_1:3599551-3599570 Msa0188410:intron 45.0%
!! AATGGAGGCATTGAAGTTGG+CGG - chr2_1:3599569-3599588 Msa0188410:intron 45.0%
TCCTAATGTCGGACACACAT+CGG + chr2_1:3599990-3600009 None:intergenic 45.0%
CCTCACTCTTGCAAAAAGGT+AGG + chr2_1:3600296-3600315 None:intergenic 45.0%
! CCTACCTTTTTGCAAGAGTG+AGG - chr2_1:3600293-3600312 Msa0188410:intron 45.0%
AAATAACCTCAACCCACCAC+GGG + chr2_1:3601166-3601185 None:intergenic 45.0%
GAAATAACCTCAACCCACCA+CGG + chr2_1:3601167-3601186 None:intergenic 45.0%
CGAAGGAAACTTTGTCTCAG+AGG + chr2_1:3601411-3601430 None:intergenic 45.0%
GTGTTGCAATTGTGAGTCGA+AGG + chr2_1:3601428-3601447 None:intergenic 45.0%
!!! ATATTTTAATATATCAATAA+TGG - chr2_1:3600513-3600532 Msa0188410:intron 5.0%
!!! TATTTTAATATATCAATAAT+GGG - chr2_1:3600514-3600533 Msa0188410:intron 5.0%
! ATTTCTGGACGCACATGCGA+TGG + chr2_1:3598877-3598896 None:intergenic 50.0%
CGCATGTGCGTCCAGAAATT+TGG - chr2_1:3598878-3598897 Msa0188410:CDS 50.0%
! GCGTTTTCTAGGTGACCTTG+TGG + chr2_1:3598964-3598983 None:intergenic 50.0%
! AGAGTGTGTGTGGGTAATGG+AGG + chr2_1:3599004-3599023 None:intergenic 50.0%
ACAAAGACCTCATCCGTGTG+AGG + chr2_1:3599090-3599109 None:intergenic 50.0%
AAGCCGCGGGAAGACATTAT+CGG + chr2_1:3599274-3599293 None:intergenic 50.0%
!! CAGGTGTTGAGACGAGGATT+CGG + chr2_1:3599307-3599326 None:intergenic 50.0%
!! AACGAACAGGTGTTGAGACG+AGG + chr2_1:3599313-3599332 None:intergenic 50.0%
CATGAACGTCGGTAACGAAC+AGG + chr2_1:3599326-3599345 None:intergenic 50.0%
CTGTTCGTTACCGACGTTCA+TGG - chr2_1:3599324-3599343 Msa0188410:intron 50.0%
! AGTTTTACCATGTCTGCCGC+GGG - chr2_1:3599358-3599377 Msa0188410:intron 50.0%
TGTTTCTCTTGGGAAACCCG+CGG + chr2_1:3599377-3599396 None:intergenic 50.0%
AAGAGGAAGAAACTCGTGGC+TGG - chr2_1:3599429-3599448 Msa0188410:intron 50.0%
!! GAACACGCAGTTTGTTGCAG+CGG - chr2_1:3599509-3599528 Msa0188410:intron 50.0%
GGAGCAGAAGAAGGATGAGT+TGG - chr2_1:3599536-3599555 Msa0188410:intron 50.0%
GTTGGAGAAGGCAGTAATGG+AGG - chr2_1:3599554-3599573 Msa0188410:intron 50.0%
GAAGTTGGCGGAAGAGAACA+AGG - chr2_1:3599581-3599600 Msa0188410:intron 50.0%
ACTATCCTCGAGGGGATGAT+TGG - chr2_1:3599619-3599638 Msa0188410:intron 50.0%
ACCGATGTGTGTCCGACATT+AGG - chr2_1:3599986-3600005 Msa0188410:intron 50.0%
TTTGTTTGGGGGAATGGCGT+CGG + chr2_1:3601203-3601222 None:intergenic 50.0%
TCTCCTTGCCGATCCTCACA+CGG - chr2_1:3599074-3599093 Msa0188410:CDS 55.0%
GACCTCATCCGTGTGAGGAT+CGG + chr2_1:3599085-3599104 None:intergenic 55.0%
CGGCACAAAAGCGAGGTATG+TGG + chr2_1:3599254-3599273 None:intergenic 55.0%
! GTGCCGATAATGTCTTCCCG+CGG - chr2_1:3599268-3599287 Msa0188410:CDS 55.0%
TCACACCAATCATCCCCTCG+AGG + chr2_1:3599627-3599646 None:intergenic 55.0%
TTCCAAGACGAGTCAACCCG+TGG - chr2_1:3601147-3601166 Msa0188410:CDS 55.0%
AAGACGAGTCAACCCGTGGT+GGG - chr2_1:3601151-3601170 Msa0188410:CDS 55.0%
CATCCGTGTGAGGATCGGCA+AGG + chr2_1:3599080-3599099 None:intergenic 60.0%
TGCCGATCCTCACACGGATG+AGG - chr2_1:3599080-3599099 Msa0188410:CDS 60.0%
GAGACGAGGATTCGGCCTCT+AGG + chr2_1:3599299-3599318 None:intergenic 60.0%
!! CACGCAGTTTGTTGCAGCGG+CGG - chr2_1:3599512-3599531 Msa0188410:intron 60.0%
!! CACCACGGGTTGACTCGTCT+TGG + chr2_1:3601152-3601171 None:intergenic 60.0%
CAAGACGAGTCAACCCGTGG+TGG - chr2_1:3601150-3601169 Msa0188410:CDS 60.0%
!! AGTCAACCCGTGGTGGGTTG+AGG - chr2_1:3601157-3601176 Msa0188410:CDS 60.0%
CTTCCCGCGGCTTGACCTAG+AGG - chr2_1:3599281-3599300 Msa0188410:intron 65.0%
TCGGCCTCTAGGTCAAGCCG+CGG + chr2_1:3599288-3599307 None:intergenic 65.0%
TGGGAAACCCGCGGCAGACA+TGG + chr2_1:3599368-3599387 None:intergenic 65.0%
!! GCAGTTTGTTGCAGCGGCGG+CGG - chr2_1:3599515-3599534 Msa0188410:intron 65.0%
CGGCCTCTAGGTCAAGCCGC+GGG + chr2_1:3599287-3599306 None:intergenic 70.0%
AGCGGCGGCGGAGCAGAAGA+AGG - chr2_1:3599527-3599546 Msa0188410:intron 70.0%
! TGGGGGAATGGCGTCGGAGC+AGG + chr2_1:3601197-3601216 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 3598856 3601559 3598856 ID=Msa0188410;Name=Msa0188410
chr2_1 mRNA 3598856 3601559 3598856 ID=Msa0188410-mRNA-1;Parent=Msa0188410;Name=Msa0188410-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|0.5|2|0|464
chr2_1 exon 3600612 3601559 3600612 ID=Msa0188410-mRNA-1:exon:1541;Parent=Msa0188410-mRNA-1
chr2_1 exon 3598856 3599299 3598856 ID=Msa0188410-mRNA-1:exon:1540;Parent=Msa0188410-mRNA-1
chr2_1 CDS 3600612 3601559 3600612 ID=Msa0188410-mRNA-1:cds;Parent=Msa0188410-mRNA-1
chr2_1 CDS 3598856 3599299 3598856 ID=Msa0188410-mRNA-1:cds;Parent=Msa0188410-mRNA-1
Gene Sequence

>Msa0188410

ATGTCTGTTCAGAAGCAACCATCGCATGTGCGTCCAGAAATTTGGCACACATGTGCTGCCGCTGCCGTCAAAATTCCAAAGTTGCATTCAAGAGTTTATTATTTCCCACAAGGTCACCTAGAAAACGCTTCTCCTTCTTCTTCTTCCTCCATTACCCACACACACTCTTTTCTACAAAGTTTTCGTCCTTTCACTCCTTGTATCGTCTCAGCTGTTGATCTCCTTGCCGATCCTCACACGGATGAGGTCTTTGTTAAACTGCTACTTACTCCAATAACCAACGATGTCCATCTTGAAAACCCTAAAGAAGAGGTTGCCAATCTCAACAACCGCGATGAAGTTGTTTCTTTTGTCAAGATTCTCACTCGTTCTGATGTTAACAATGCTCGGTCATTCCACATACCTCGCTTTTGTGCCGATAATGTCTTCCCGCGGCTTGACCTAGAGGCCGAATCCTCGTCTCAACACCTGTTCGTTACCGACGTTCATGGTGAAGTTTCAAAGTTTTACCATGTCTGCCGCGGGTTTCCCAAGAGAAACATGTTATATATCTCTGAATGGAATTCCTTTGTTAAGAGGAAGAAACTCGTGGCTGGAGATTCCGTTATTTTCATGAAAGACTCAACGGGAAAGTTATTTGTTGGGATTCGCAGGAACACGCAGTTTGTTGCAGCGGCGGCGGAGCAGAAGAAGGATGAGTTGGAGAAGGCAGTAATGGAGGCATTGAAGTTGGCGGAAGAGAACAAGGCATTTGAAATTGTTTACTATCCTCGAGGGGATGATTGGTGTGATTTTGTAGTGGATGGTAATGCTGTTGATGAATCAATGAAGATTCAATGGAACCCTAGAATGAGAGTTAAGATGAAGACTGATAAATCTTCAAGGATCCCATATCAAGGGACAATCTCTATTGTATCTCGTACTTCTAATTTATGGCGCATGCTTCAGGTTAATTGGGATGAATTTCAAGTCTCGGAAATTCCAAGACGAGTCAACCCGTGGTGGGTTGAGGTTATTTCTCACAAACCTGCTCCGACGCCATTCCCCCAAACAAAAAAATTTAGAACTACTCAAAGTTCTGCCCAACTAAGTGATAAAAAGGAAACATTATTGAATGATGATGCTTCAAACATTTTGAATGAGATCACTTCAACGGAAGGGTTAAGTAATGTGTCAAAAGAATTAAACATGAGTCCTATACATGATAAAGTATCACCTCACAGCCATATTATTTTGAACTCCTCTGAGACAAAGTTTCCTTCGACTCACAATTGCAACACCAAAAATTATAGTGATGGTTCAATTATGTTATTTGGTAAGATCATACAGCCTCATGTAAGCAATTTTCACAATTCTCATATCAAGGGAGATGATGGCTATATAAGAGCTGCG
Protein sequence

>Msa0188410

MSVQKQPSHVRPEIWHTCAAAAVKIPKLHSRVYYFPQGHLENASPSSSSSITHTHSFLQSFRPFTPCIVSAVDLLADPHTDEVFVKLLLTPITNDVHLENPKEEVANLNNRDEVVSFVKILTRSDVNNARSFHIPRFCADNVFPRLDLEAESSSQHLFVTDVHGEVSKFYHVCRGFPKRNMLYISEWNSFVKRKKLVAGDSVIFMKDSTGKLFVGIRRNTQFVAAAAEQKKDELEKAVMEALKLAEENKAFEIVYYPRGDDWCDFVVDGNAVDESMKIQWNPRMRVKMKTDKSSRIPYQGTISIVSRTSNLWRMLQVNWDEFQVSEIPRRVNPWWVEVISHKPAPTPFPQTKKFRTTQSSAQLSDKKETLLNDDASNILNEITSTEGLSNVSKELNMSPIHDKVSPHSHIILNSSETKFPSTHNCNTKNYSDGSIMLFGKIIQPHVSNFHNSHIKGDDGYIRAA