AlfalfaGEDB | Msa0192930

M. sativa cultivar zhongmu-4 / Msa0192930

KEGG: K17604
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0192930	MCH82909.1	69.583	240	72	1	18	257	4	242	8.52e-120	357

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0192930	sp\|Q6NQJ7\|FRS4_ARATH	34.112	214	115	6	48	250	3	201	1.62e-29	120

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0192930	A0A392M6P6	69.583	240	72	1	18	257	4	242	4.07e-120	357

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TFs/TRs:

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Gene ID	Type	Classification
Msa0192930	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0192930	MtrunA17_Chr2g0296781	75.238	210	52	0	48	257	3	212	1.13e-113	342
Msa0192930	MtrunA17_Chr1g0196541	57.143	210	90	0	48	257	3	212	2.40e-81	258
Msa0192930	MtrunA17_Chr6g0477261	88.119	101	12	0	157	257	1	101	1.86e-57	194
Msa0192930	MtrunA17_Chr5g0441581	38.983	236	128	5	28	257	15	240	1.05e-42	154
Msa0192930	MtrunA17_Chr6g0476431	61.628	86	33	0	48	133	3	88	5.63e-34	118
Msa0192930	MtrunA17_Chr2g0329031	33.175	211	120	5	48	250	20	217	1.68e-29	116
Msa0192930	MtrunA17_Chr2g0291591	30.204	245	162	5	18	257	39	279	1.58e-28	113
Msa0192930	MtrunA17_Chr6g0451441	32.500	200	125	5	10	203	52	247	4.76e-24	100
Msa0192930	MtrunA17_Chr7g0258861	28.194	227	152	4	39	257	7	230	6.05e-24	98.2
Msa0192930	MtrunA17_Chr1g0207551	29.018	224	137	6	39	250	31	244	1.42e-23	99.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0192930	AT1G76320.3	34.112	214	115	6	48	250	3	201	1.52e-30	120
Msa0192930	AT1G76320.2	34.112	214	115	6	48	250	3	201	1.64e-30	120
Msa0192930	AT1G76320.1	34.112	214	115	6	48	250	3	201	1.65e-30	120
Msa0192930	AT1G76320.4	34.112	214	115	6	48	250	3	201	1.65e-30	120
Msa0192930	AT4G15090.1	30.435	230	139	6	39	255	48	269	2.02e-27	111
Msa0192930	AT4G15090.5	30.435	230	139	6	39	255	48	269	2.02e-27	111
Msa0192930	AT4G15090.3	30.435	230	139	6	39	255	48	269	2.02e-27	111
Msa0192930	AT4G15090.2	30.435	230	139	6	39	255	48	269	2.02e-27	111
Msa0192930	AT4G15090.4	30.435	230	139	6	39	255	48	269	2.02e-27	111
Msa0192930	AT1G52520.1	30.483	269	155	9	9	257	37	293	3.78e-20	90.1

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Find 30 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTGAGGAGACTACGACT+TGG	0.649574	2_1:-8749613	Msa0192930:CDS
AACTCCAAAACCCATAGATT+GGG	0.473331	2_1:+8750029	None:intergenic
AAGAGTTATGCCCAATCTAT+GGG	0.502102	2_1:-8750039	Msa0192930:CDS
AAGTGAGGAGACTACGACTT+GGG	0.414907	2_1:-8749612	Msa0192930:CDS
AATGCATGTATTTCTTTAGA+TGG	0.249459	2_1:-8749865	Msa0192930:CDS
ACTTGGGGCTGGAGATGCTG+AGG	0.650883	2_1:-8749596	Msa0192930:CDS
AGGAGACTACGACTTGGGGC+TGG	0.514634	2_1:-8749607	Msa0192930:CDS
AGTGAGGAGACTACGACTTG+GGG	0.641932	2_1:-8749611	Msa0192930:CDS
ATGAAGAGGAAATCAGAGAG+GGG	0.681620	2_1:-8750167	Msa0192930:CDS
ATTTAATCTAGCCTTGCACT+GGG	0.609429	2_1:+8749885	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATGGGTAATAACCCAATTTA+AGG	+	chr2_1:8749496-8749515	None:intergenic	30.0%
ATTTGTATCTTCTGATGAAG+AGG	-	chr2_1:8749713-8749732	Msa0192930:CDS	30.0%
GGTTATGAGAATATGACATT+TGG	-	chr2_1:8750230-8750249	Msa0192930:intron	30.0%
ATGGGTAATAACCCAATTTA+AGG	+	chr2_1:8749496-8749515	None:intergenic	30.0%
ATTTGTATCTTCTGATGAAG+AGG	-	chr2_1:8749713-8749732	Msa0192930:CDS	30.0%
GGTTATGAGAATATGACATT+TGG	-	chr2_1:8750230-8750249	Msa0192930:intron	30.0%
TCATGAAGATGAAAAACCTC+AGG	-	chr2_1:8749788-8749807	Msa0192930:CDS	35.0%
CTAAATATAGTTCCAACCTG+AGG	+	chr2_1:8749807-8749826	None:intergenic	35.0%
AAGAGTTATGCCCAATCTAT+GGG	-	chr2_1:8749855-8749874	Msa0192930:CDS	35.0%
AACTCCAAAACCCATAGATT+GGG	+	chr2_1:8749868-8749887	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	8749459	8750457	8749459	ID=Msa0192930;Name=Msa0192930
chr2_1	mRNA	8749459	8750457	8749459	ID=Msa0192930-mRNA-1;Parent=Msa0192930;Name=Msa0192930-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0\|0\|0\|0.5\|1\|1\|2\|0\|258
chr2_1	exon	8750436	8750457	8750436	ID=Msa0192930-mRNA-1:exon:4026;Parent=Msa0192930-mRNA-1
chr2_1	exon	8749459	8750213	8749459	ID=Msa0192930-mRNA-1:exon:4025;Parent=Msa0192930-mRNA-1
chr2_1	CDS	8750436	8750457	8750436	ID=Msa0192930-mRNA-1:cds;Parent=Msa0192930-mRNA-1
chr2_1	CDS	8749459	8750213	8749459	ID=Msa0192930-mRNA-1:cds;Parent=Msa0192930-mRNA-1

Gene Sequence

>Msa0192930

ATGGAAACAGTGCAAAAATCAATGGAAGAAGTATTTGTATCTTCTGATGAAGAGGAAATCAGAGAGGGGAATAATAATTTTGATGATAGTTTAGAAGAAGATATTGTTCATGAAGATGAAAAACCTCAGGTTGGAACTATATTTAGCTCTAAAGATGAACTTTTTGAGTATTACAAGAGTTATGCCCAATCTATGGGTTTTGGAGTTGCTAAACTTAGTAACAAAAATACAGATGATGGGAAAAAATACTTTACTCTAGCATGTAGTCGTGGATCAAAGTATGTGAGTAAGGCGAAGAAGCTTTTGAACCCAAATCCTTTAACAAAAACCCAGTGCAAGGCTAGATTAAATGCATGTATTTCTTTAGATGGAACAGTTACTATTACAAAACTTTTACTTGAGCATAATCATGATCTAATCCCTAACAAAGCACGCTATTTTAGAAGTAGTAAGTTTTTGGATCCTCGTTTGAAAAGGAGGTTAGAACTCAATGATCAAGCTGGAATAAATGTAAGCAGAAATTTTCGATCAATGGTTGTTGAAGCAAATGGTTATGAGAATATGACATTTGGTGAAAAGGACTATAGAAACTATATAGATAAAGTGAGGAGACTACGACTTGGGGCTGGAGATGCTGAGGCTATTCAAAATTATTTCGTTAAAATGCAAAAGCAAAATAGTCAGTTCTATTATGTAATAGACATTGATGATAAAAGTCGCTTACGAAATGTTTTTTGGGCAGATGCGAGATGTAGGGCTGCATATGAATATGTCTAA

Protein sequence

>Msa0192930

METVQKSMEEVFVSSDEEEIREGNNNFDDSLEEDIVHEDEKPQVGTIFSSKDELFEYYKSYAQSMGFGVAKLSNKNTDDGKKYFTLACSRGSKYVSKAKKLLNPNPLTKTQCKARLNACISLDGTVTITKLLLEHNHDLIPNKARYFRSSKFLDPRLKRRLELNDQAGINVSRNFRSMVVEANGYENMTFGEKDYRNYIDKVRRLRLGAGDAEAIQNYFVKMQKQNSQFYYVIDIDDKSRLRNVFWADARCRAAYEYV*