AlfalfaGEDB | Msa0196660

M. sativa cultivar zhongmu-4 / Msa0196660

KEGG: K08066|K19681
GO: GO:0000429 GO:0000436 GO:0000902 GO:0000904 GO:0000976 GO:0000977 GO:0000978 GO:0000981 GO:0000982 GO:0000987 GO:0001012 GO:0001067 GO:0001077 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003712 GO:0003713 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006457 GO:0006725 GO:0006807 GO:0006928 GO:0006935 GO:0007154 GO:0007275 GO:0007399 GO:0007409 GO:0007411 GO:0007584 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009605 GO:0009653 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0009991 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0016602 GO:0018130 GO:0019216 GO:0019218 GO:0019219 GO:0019222 GO:0019438 GO:0022008 GO:0030030 GO:0030154 GO:0030182 GO:0031175 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031667 GO:0031668 GO:0031669 GO:0031670 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032989 GO:0032990 GO:0032991 GO:0032993 GO:0034641 GO:0034645 GO:0034654 GO:0040011 GO:0042221 GO:0042330 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043457 GO:0043467 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044798 GO:0045540 GO:0045893 GO:0045935 GO:0045944 GO:0045990 GO:0045991 GO:0046483 GO:0046890 GO:0048468 GO:0048518 GO:0048522 GO:0048666 GO:0048667 GO:0048699 GO:0048731 GO:0048812 GO:0048856 GO:0048858 GO:0048869 GO:0050789 GO:0050794 GO:0050810 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0061564 GO:0062012 GO:0065007 GO:0070013 GO:0070887 GO:0071496 GO:0071704 GO:0071840 GO:0080090 GO:0090181 GO:0090304 GO:0090575 GO:0097159 GO:0097485 GO:0097659 GO:0106118 GO:0120036 GO:0120039 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1902930 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
PFAM: PF00808.26

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0196660	XP_013462829.1	70.652	184	25	2	7	166	66	244	3.94e-82	253

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0196660	sp\|Q9ZVL3\|NFYC3_ARATH	49.701	167	59	4	7	152	51	213	3.46e-45	150

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0196660	I3TAY7	70.652	184	25	2	7	166	66	244	1.88e-82	253

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TFs/TRs:

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Gene ID	Type	Classification
Msa0196660	TF	NF-YC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0196660	MtrunA17_Chr2g0288481	70.652	184	25	2	7	166	66	244	3.63e-86	253
Msa0196660	MtrunA17_Chr2g0310741	54.745	137	58	2	8	144	85	217	2.28e-49	160
Msa0196660	MtrunA17_Chr7g0272991	47.904	167	63	3	7	149	82	248	5.37e-47	153
Msa0196660	MtrunA17_Chr1g0191681	66.667	99	33	0	7	105	82	180	2.69e-46	151
Msa0196660	MtrunA17_Chr3g0132191	52.555	137	52	2	8	131	37	173	2.21e-44	145
Msa0196660	MtrunA17_Chr8g0376351	94.444	72	4	0	44	115	1	72	3.70e-44	140
Msa0196660	MtrunA17_Chr2g0318881	59.740	77	30	1	27	102	23	99	1.88e-24	91.7
Msa0196660	MtrunA17_Chr2g0318921	59.740	77	30	1	27	102	23	99	2.24e-24	91.7
Msa0196660	MtrunA17_Chr3g0081161	42.308	78	45	0	27	104	9	86	7.86e-13	64.7
Msa0196660	MtrunA17_Chr8g0354151	39.744	78	47	0	27	104	9	86	3.39e-12	63.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0196660	AT1G54830.2	49.701	167	59	4	7	152	51	213	3.52e-46	150
Msa0196660	AT1G54830.3	49.701	167	59	4	7	152	51	213	3.52e-46	150
Msa0196660	AT1G54830.1	49.701	167	59	4	7	152	51	213	3.52e-46	150
Msa0196660	AT1G56170.1	56.452	124	52	1	5	128	55	176	3.56e-46	150
Msa0196660	AT1G56170.2	56.452	124	52	1	5	128	55	176	3.56e-46	150
Msa0196660	AT1G08970.3	64.815	108	36	1	8	115	62	167	3.16e-45	148
Msa0196660	AT1G08970.4	64.815	108	36	1	8	115	62	167	3.16e-45	148
Msa0196660	AT1G08970.2	64.815	108	36	1	8	115	62	167	3.16e-45	148
Msa0196660	AT1G08970.1	64.815	108	36	1	8	115	62	167	3.16e-45	148
Msa0196660	AT3G48590.1	50.000	144	52	2	8	131	47	190	6.52e-45	147

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Find 32 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGATCATGAAAACAGAAGA+AGG	0.505724	2_1:-14013158	Msa0196660:CDS
AAGGTCATGGGCTAATACAG+AGG	0.637560	2_1:-14013060	Msa0196660:CDS
AATGGAGCGTGACATATTTA+TGG	0.230451	2_1:-14012937	Msa0196660:CDS
AATGTTTCCAATCCAAAAGC+AGG	0.532891	2_1:-14012729	Msa0196660:CDS
AATTTGGAAAATCAACATAA+TGG	0.484356	2_1:+14012699	None:intergenic
ACTGTTGATTTCAAAAGTAA+TGG	0.437192	2_1:-14013203	Msa0196660:CDS
AGTCCTCCAAGAATGCTTAT+GGG	0.511667	2_1:-14012812	Msa0196660:CDS
AGTGAGTATGATATCAGCTG+AGG	0.690629	2_1:-14013135	Msa0196660:CDS
ATCTTCTTAATCCTTGCTAA+TGG	0.378288	2_1:+14013176	None:intergenic
ATGGAGCGTGACATATTTAT+GGG	0.438860	2_1:-14012936	Msa0196660:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATCTTCTTAATCCTTGCTAA+TGG	+	chr2_1:14012772-14012791	None:intergenic	30.0%
AAGATCATGAAAACAGAAGA+AGG	-	chr2_1:14012787-14012806	Msa0196660:CDS	30.0%
GGCATGTGAAATGTTTATTA+TGG	-	chr2_1:14012852-14012871	Msa0196660:intron	30.0%
AGGAACATATATTCCCTATT+GGG	+	chr2_1:14013056-14013075	None:intergenic	30.0%
AAGGAACATATATTCCCTAT+TGG	+	chr2_1:14013057-14013076	None:intergenic	30.0%
AGAAAAAACTTGACACCTTC+TGG	-	chr2_1:14012692-14012711	Msa0196660:CDS	35.0%
TTATTATGGAGCTAGCAACA+AGG	-	chr2_1:14012866-14012885	Msa0196660:intron	35.0%
AATGGAGCGTGACATATTTA+TGG	-	chr2_1:14013008-14013027	Msa0196660:CDS	35.0%
ATGGAGCGTGACATATTTAT+GGG	-	chr2_1:14013009-14013028	Msa0196660:CDS	35.0%
ATTCTTGGAGGACTATATGA+TGG	+	chr2_1:14013127-14013146	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	14012688	14013279	14012688	ID=Msa0196660;Name=Msa0196660
chr2_1	mRNA	14012688	14013279	14012688	ID=Msa0196660-mRNA-1;Parent=Msa0196660;Name=Msa0196660-mRNA-1;_AED=0.18;_eAED=0.18;_QI=0\|0\|0\|1\|1\|1\|2\|0\|166
chr2_1	exon	14012937	14013279	14012937	ID=Msa0196660-mRNA-1:exon:6044;Parent=Msa0196660-mRNA-1
chr2_1	exon	14012688	14012845	14012688	ID=Msa0196660-mRNA-1:exon:6043;Parent=Msa0196660-mRNA-1
chr2_1	CDS	14012937	14013279	14012937	ID=Msa0196660-mRNA-1:cds;Parent=Msa0196660-mRNA-1
chr2_1	CDS	14012688	14012845	14012688	ID=Msa0196660-mRNA-1:cds;Parent=Msa0196660-mRNA-1

Gene Sequence

>Msa0196660

TTGCAGAAAAAACTTGACACCTTCTGGTCAAAACAAAATGAAGAAGTTCAGAAGACTGTTGATTTCAAAAGTAATGGCTTGCCATTAGCAAGGATTAAGAAGATCATGAAAACAGAAGAAGGAGTGAGTATGATATCAGCTGAGGCACCTATTCTGTTTGCTAAGGCATGTGAAATGTTTATTATGGAGCTAGCAACAAGGTCATGGGCTAATACAGAGGTGAACAAAAGAAAGACACTTCAAAAGAGTGACATTGCATCTGCTGTCTCAAGTAATGAAGTGTTTGATTTCCTAGTTGATATTGTCCCTAGGGAAAAAACAATGGAGCGTGACATATTTATGGGTCCATCATATAGTCCTCCAAGAATGCTTATGGGAACACCTTCTCTCAATCAGCCTCATAACAACAGGCAGCCCTCACACCCTCTTGCAAATCAAATGTTTCCAATCCAAAAGCAGGAAAATGACCATTATGTTGATTTTCCAAATTCAGAAGATTAA

Protein sequence

>Msa0196660

LQKKLDTFWSKQNEEVQKTVDFKSNGLPLARIKKIMKTEEGVSMISAEAPILFAKACEMFIMELATRSWANTEVNKRKTLQKSDIASAVSSNEVFDFLVDIVPREKTMERDIFMGPSYSPPRMLMGTPSLNQPHNNRQPSHPLANQMFPIQKQENDHYVDFPNSED*