AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0199480

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0199480 MtrunA17_Chr2g0293901 81.176 170 29 1 1 167 1 170 4.99e-63 202
Msa0199480 MtrunA17_Chr8g0366921 45.152 330 152 8 1 311 1 320 1.01e-56 189
Msa0199480 MtrunA17_Chr7g0266621 47.134 314 149 5 1 309 1 302 1.54e-45 159
Msa0199480 MtrunA17_Chr8g0368721 50.470 319 143 6 1 311 1 312 4.40e-44 156
Msa0199480 MtrunA17_Chr6g0470151 45.260 327 147 9 1 311 1 311 1.01e-39 146
Msa0199480 MtrunA17_Chr2g0289181 37.500 200 45 3 311 430 34 233 2.73e-32 122
Msa0199480 MtrunA17_Chr6g0470161 55.072 69 31 0 362 430 52 120 1.25e-20 87.0
Msa0199480 MtrunA17_Chr7g0266611 63.077 65 23 1 366 430 102 165 3.98e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 96 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTCTGAAACAGAATTTAT+TGG 0.120089 2_1:+18521765 Msa0199480:CDS
TAGAAACCCAAACTAGTTCT+AGG 0.250090 2_1:+18520182 Msa0199480:CDS
ACAAAGTTCTTAATTTCTTC+TGG 0.267372 2_1:-18521651 None:intergenic
GTCGGGGTGGTGGTGGTTGA+AGG 0.269792 2_1:-18519647 None:intergenic
GTAAAACCCTAGAACTAGTT+TGG 0.270284 2_1:-18520189 None:intergenic
AAGGTGGTGGTAGTGGTAAT+TGG 0.290628 2_1:-18519872 None:intergenic
CTAGTTTGGGTTTCTAGAAT+TGG 0.312695 2_1:-18520175 None:intergenic
CAAACCTCAGATCAAGAAAA+AGG 0.317679 2_1:+18520233 Msa0199480:CDS
GGTGGTGGTGGTTGAAGGGG+TGG 0.333560 2_1:-18519642 None:intergenic
AGGTGGTGGTAGTGGTAATT+GGG 0.338809 2_1:-18519871 None:intergenic
GGTAATTGGGGTAATGATAG+TGG 0.346701 2_1:-18519858 None:intergenic
AGGGATAATTGTGGTGGGAT+TGG 0.353569 2_1:-18519582 None:intergenic
ATACGGGTGCTAGCGGGGTT+AGG 0.359448 2_1:-18519602 None:intergenic
TGGTGGTGGTTGAAGGGGTG+GGG 0.363221 2_1:-18519640 None:intergenic
CGGGGTGGTGGTGGTTGAAG+GGG 0.364013 2_1:-18519645 None:intergenic
CTTCAATGGATAATGATGAT+TGG 0.367851 2_1:+18519318 None:intergenic
TATTATTAGTGATGTGGGTC+GGG 0.369132 2_1:-18519664 None:intergenic
TTCAATGGATAATGATGATT+GGG 0.373937 2_1:+18519319 None:intergenic
GGGAATGTGAATAGCGGTGG+TGG 0.376751 2_1:-18519813 None:intergenic
GCTTATATTGTGACGTATAA+AGG 0.379319 2_1:+18521609 Msa0199480:CDS
GTGGTGGTGGTTAGGATTTG+AGG 0.379807 2_1:-18519419 None:intergenic
GATTGTTGCTGATTTGTGTT+TGG 0.383802 2_1:-18520211 None:intergenic
GTGGTGGTGGTTGAAGGGGT+GGG 0.384113 2_1:-18519641 None:intergenic
TCTAACTCTATGAAGTCATT+TGG 0.386725 2_1:-18519542 None:intergenic
TACTGATGGTGGTGGTGGTT+AGG 0.393621 2_1:-18519427 None:intergenic
TCTGACATAGCTGTTATGTT+TGG 0.394725 2_1:-18521819 None:intergenic
AGTGATGTGGGTCGGGGTGG+TGG 0.395367 2_1:-18519657 None:intergenic
TAAAACCCTAGAACTAGTTT+GGG 0.406708 2_1:-18520188 None:intergenic
AATTGTGGTGGGATTGGAAT+TGG 0.413486 2_1:-18519576 None:intergenic
TCGGGGTGGTGGTGGTTGAA+GGG 0.422426 2_1:-18519646 None:intergenic
ACTAACACTAGGATATTGAT+TGG 0.429065 2_1:-18519990 None:intergenic
GAAATTAAGAACTTTGTTGT+TGG 0.434538 2_1:+18521657 Msa0199480:CDS
TATGTCAGAAGATTTATTGA+TGG 0.435423 2_1:+18521833 Msa0199480:CDS
GATGTGGGTCGGGGTGGTGG+TGG 0.445392 2_1:-18519654 None:intergenic
TACGGGTGCTAGCGGGGTTA+GGG 0.449094 2_1:-18519601 None:intergenic
TGGAGTTAGAGTTTCAGAAT+TGG 0.449537 2_1:-18519396 None:intergenic
TGTGTATGTTGTGGACTAGA+TGG 0.450832 2_1:-18520136 None:intergenic
AAAGGTGGAATTTCGATTTG+AGG 0.455769 2_1:-18519705 None:intergenic
GACTTTAACCACCTCAATAG+TGG 0.457259 2_1:+18521855 Msa0199480:CDS
GGAATGTGAATAGCGGTGGT+GGG 0.459869 2_1:-18519812 None:intergenic
ACCTGAGAATTGCTAGTCTC+TGG 0.471222 2_1:-18521717 None:intergenic
GAGCATAACATCTTCTACTT+CGG 0.472162 2_1:-18519780 None:intergenic
GTTAATATTATTAGTGATGT+GGG 0.476825 2_1:-18519669 None:intergenic
GCCAGAGACTAGCAATTCTC+AGG 0.477878 2_1:+18521716 Msa0199480:CDS
TGAAGTCATTTGGTTTAAGC+GGG 0.482952 2_1:-18519532 None:intergenic
AGCGGTTGTGATTTCAATGC+AGG 0.486927 2_1:-18520089 None:intergenic
TTTGGAATACGGGTGCTAGC+GGG 0.488713 2_1:-18519608 None:intergenic
ATATTATTAGTGATGTGGGT+CGG 0.491960 2_1:-18519665 None:intergenic
ATGAAGTCATTTGGTTTAAG+CGG 0.492100 2_1:-18519533 None:intergenic
TTGCTTCTTTGTGTATGTTG+TGG 0.506190 2_1:-18520145 None:intergenic
AGAAACCCAAACTAGTTCTA+GGG 0.507577 2_1:+18520183 Msa0199480:CDS
GTTAGCGAAGGTGGTGGTAG+TGG 0.510257 2_1:-18519879 None:intergenic
ATTATTAGTGATGTGGGTCG+GGG 0.521424 2_1:-18519663 None:intergenic
GAATGTGAATAGCGGTGGTG+GGG 0.524266 2_1:-18519811 None:intergenic
GGTAGGAGTAGTGGCAGTCA+AGG 0.527115 2_1:-18519921 None:intergenic
CAAGGTGCTAATAGTGTTGG+CGG 0.530004 2_1:-18519903 None:intergenic
AGTTAGAGTTTCAGAATTGG+TGG 0.535695 2_1:-18519393 None:intergenic
GGAATAAAGCACCACTATTG+AGG 0.537769 2_1:-18521866 None:intergenic
GTTGGCGGGGTTAGCGAAGG+TGG 0.541707 2_1:-18519888 None:intergenic
TGTTAATATTATTAGTGATG+TGG 0.542659 2_1:-18519670 None:intergenic
GGTGGTGAGAGTAGTGATGG+TGG 0.545701 2_1:-18519834 None:intergenic
GATAGTGTTGGCAGTAGAGA+TGG 0.547801 2_1:-18519942 None:intergenic
GTAATTGGGGTAATGATAGT+GGG 0.548235 2_1:-18519857 None:intergenic
GATGTTACTGATGGTGGTGG+TGG 0.548240 2_1:-18519432 None:intergenic
GGCGGGGTTAGCGAAGGTGG+TGG 0.550477 2_1:-18519885 None:intergenic
AAGGTGCTAATAGTGTTGGC+GGG 0.550983 2_1:-18519902 None:intergenic
GTGGTGGTTAGGATTTGAGG+TGG 0.552967 2_1:-18519416 None:intergenic
AGTAGAGATGGTAGGAGTAG+TGG 0.558152 2_1:-18519930 None:intergenic
GGGTTAGGGATAATTGTGGT+GGG 0.569034 2_1:-18519587 None:intergenic
GGATATGATGTTACTGATGG+TGG 0.569473 2_1:-18519438 None:intergenic
GGTGGCAGAAGTAGTAAAGG+TGG 0.572647 2_1:-18519375 None:intergenic
ATTGGTGGCAGAAGTAGTAA+AGG 0.577716 2_1:-18519378 None:intergenic
GTGGTGAGAGTAGTGATGGT+GGG 0.578827 2_1:-18519833 None:intergenic
AGTCAAGGTGCTAATAGTGT+TGG 0.585343 2_1:-18519906 None:intergenic
TGTTGTTGTTCAATGAGTGT+TGG 0.587742 2_1:-18519737 None:intergenic
GTGTTGGCAGTAGAGATGGT+AGG 0.592781 2_1:-18519938 None:intergenic
ATTAGTGATGTGGGTCGGGG+TGG 0.595677 2_1:-18519660 None:intergenic
TTGGAATACGGGTGCTAGCG+GGG 0.599630 2_1:-18519607 None:intergenic
GGGGTTAGGGATAATTGTGG+TGG 0.606726 2_1:-18519588 None:intergenic
GGTGGGAATGTGAATAGCGG+TGG 0.610840 2_1:-18519816 None:intergenic
AGTGTTCGTCATGATAGTGT+TGG 0.615034 2_1:-18519954 None:intergenic
AATTGGGGTAATGATAGTGG+GGG 0.618091 2_1:-18519855 None:intergenic
TAATTGGGGTAATGATAGTG+GGG 0.618793 2_1:-18519856 None:intergenic
ATCATCATAGCATGATGATG+TGG 0.621279 2_1:-18519471 None:intergenic
TATGTTGTGGACTAGATGGA+TGG 0.621555 2_1:-18520132 None:intergenic
TGATGATGTGGTACTTTGAG+AGG 0.630706 2_1:-18519459 None:intergenic
GGTGGTGGTAGTGGTAATTG+GGG 0.635605 2_1:-18519870 None:intergenic
TATGATGTTACTGATGGTGG+TGG 0.635627 2_1:-18519435 None:intergenic
AGTGTTGGCGGGGTTAGCGA+AGG 0.641015 2_1:-18519891 None:intergenic
AGAGGATATGATGTTACTGA+TGG 0.644683 2_1:-18519441 None:intergenic
ATAAAGCACCACTATTGAGG+TGG 0.665488 2_1:-18521863 None:intergenic
GAAGTCATTTGGTTTAAGCG+GGG 0.666201 2_1:-18519531 None:intergenic
AGCGGGGTTAGGGATAATTG+TGG 0.672234 2_1:-18519591 None:intergenic
AGGTGCTAATAGTGTTGGCG+GGG 0.672241 2_1:-18519901 None:intergenic
GATGGTGGGAATGTGAATAG+CGG 0.710352 2_1:-18519819 None:intergenic
GTATGCACGATCAAAATGAG+CGG 0.723045 2_1:-18520107 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAATAAAAAATATATAT+AGG + chr2_1:18520933-18520952 Msa0199480:intron 0.0%
!!! TTTATTTTATTTTTTGTCTT+TGG - chr2_1:18520923-18520942 None:intergenic 10.0%
!! ATGGATAAAAATTCATTAAA+GGG + chr2_1:18520467-18520486 Msa0199480:intron 15.0%
!!! ATAGAAAAAGTAATTTTACA+GGG - chr2_1:18520685-18520704 None:intergenic 15.0%
!!! AATAGAAAAAGTAATTTTAC+AGG - chr2_1:18520686-18520705 None:intergenic 15.0%
!! TTAATATGAATAATATTTGC+AGG + chr2_1:18520851-18520870 Msa0199480:intron 15.0%
!! TAATATGAATAATATTTGCA+GGG + chr2_1:18520852-18520871 Msa0199480:intron 15.0%
!! GTTAATATTATTAGTGATGT+GGG - chr2_1:18519672-18519691 None:intergenic 20.0%
!! TGTTAATATTATTAGTGATG+TGG - chr2_1:18519673-18519692 None:intergenic 20.0%
!!! TTTTTTCTGTAAGAAAAGAA+AGG - chr2_1:18520373-18520392 None:intergenic 20.0%
!! CATGGATAAAAATTCATTAA+AGG + chr2_1:18520466-18520485 Msa0199480:intron 20.0%
!!! AAATAGTGACAATAAACTTT+TGG + chr2_1:18520607-18520626 Msa0199480:intron 20.0%
!! AAAGTGAAAGATATAAACTT+AGG - chr2_1:18521060-18521079 None:intergenic 20.0%
!! TGATAAACAAACAATTGATA+CGG + chr2_1:18521094-18521113 Msa0199480:intron 20.0%
!!! TTTATATAAAGGTATGCAAT+CGG - chr2_1:18521209-18521228 None:intergenic 20.0%
!! AAGAGATTGCATTATTTTCT+TGG - chr2_1:18519508-18519527 None:intergenic 25.0%
!!! GATAATGATTTTTGGAATAC+GGG - chr2_1:18519621-18519640 None:intergenic 25.0%
!!! GGATAATGATTTTTGGAATA+CGG - chr2_1:18519622-18519641 None:intergenic 25.0%
! GTAAGAAAAGAAAGGTAAAA+TGG - chr2_1:18520365-18520384 None:intergenic 25.0%
!! TTTTGAATGTATGAAAACCT+TGG - chr2_1:18520502-18520521 None:intergenic 25.0%
!! TTGTCACTATTTAAAACATG+TGG - chr2_1:18520599-18520618 None:intergenic 25.0%
! TATCAGTGCTCTTTATATAA+AGG - chr2_1:18521220-18521239 None:intergenic 25.0%
! TTAATCATTCTTTGCAATCT+TGG - chr2_1:18521318-18521337 None:intergenic 25.0%
!! TGATTTGTATCTTTAACAGT+AGG + chr2_1:18521345-18521364 Msa0199480:intron 25.0%
! TCTTTAACAGTAGGAAAATA+CGG + chr2_1:18521354-18521373 Msa0199480:intron 25.0%
!! GAAATCTATGAATGATTTGT+AGG + chr2_1:18521511-18521530 Msa0199480:intron 25.0%
!!! TTCTCTACATATTTTTTTGC+TGG - chr2_1:18521570-18521589 None:intergenic 25.0%
!!! TCTTAATTTCTTCTGGTTTT+TGG - chr2_1:18521647-18521666 None:intergenic 25.0%
! ACAAAGTTCTTAATTTCTTC+TGG - chr2_1:18521654-18521673 None:intergenic 25.0%
!! GAAATTAAGAACTTTGTTGT+TGG + chr2_1:18521657-18521676 Msa0199480:CDS 25.0%
!!! ACAAGAGATTTTTGATTCAT+CGG - chr2_1:18521748-18521767 None:intergenic 25.0%
!!! TGTTCTGAAACAGAATTTAT+TGG + chr2_1:18521765-18521784 Msa0199480:CDS 25.0%
! TATGTCAGAAGATTTATTGA+TGG + chr2_1:18521833-18521852 Msa0199480:CDS 25.0%
! ATGAAGTCATTTGGTTTAAG+CGG - chr2_1:18519536-18519555 None:intergenic 30.0%
TCTAACTCTATGAAGTCATT+TGG - chr2_1:18519545-18519564 None:intergenic 30.0%
ATATTATTAGTGATGTGGGT+CGG - chr2_1:18519668-18519687 None:intergenic 30.0%
ACTAACACTAGGATATTGAT+TGG - chr2_1:18519993-18520012 None:intergenic 30.0%
GTTGAAAAATCACTAACACT+AGG - chr2_1:18520004-18520023 None:intergenic 30.0%
! TAAAACCCTAGAACTAGTTT+GGG - chr2_1:18520191-18520210 None:intergenic 30.0%
! ATTACCTTTTTCTTGATCTG+AGG - chr2_1:18520240-18520259 None:intergenic 30.0%
! GTTCGATGAGATTTAAGTTA+TGG - chr2_1:18520337-18520356 None:intergenic 30.0%
! CTATTTAAAACATGTGGTGT+GGG - chr2_1:18520593-18520612 None:intergenic 30.0%
! ACTATTTAAAACATGTGGTG+TGG - chr2_1:18520594-18520613 None:intergenic 30.0%
ATCCTTGCAATTTCAAAGAA+TGG + chr2_1:18520630-18520649 Msa0199480:intron 30.0%
!! TACCATTCTTTGAAATTGCA+AGG - chr2_1:18520635-18520654 None:intergenic 30.0%
AAGAACTATAACAAACACCA+GGG - chr2_1:18520740-18520759 None:intergenic 30.0%
TAAGAACTATAACAAACACC+AGG - chr2_1:18520741-18520760 None:intergenic 30.0%
TAAAAACCAAAAGCCAAAGA+TGG + chr2_1:18520890-18520909 Msa0199480:intron 30.0%
!!! AATAAACCATCTTTGGCTTT+TGG - chr2_1:18520899-18520918 None:intergenic 30.0%
! CTTTGGAAATAAACCATCTT+TGG - chr2_1:18520906-18520925 None:intergenic 30.0%
ACACATATATATGCCATGAT+GGG + chr2_1:18520963-18520982 Msa0199480:intron 30.0%
!! ATATGTTTTTGATCCCATCA+TGG - chr2_1:18520979-18520998 None:intergenic 30.0%
TTGCTCAATGGAAAAAGATA+CGG - chr2_1:18521277-18521296 None:intergenic 30.0%
! CTTTTTCCATTGAGCAATTA+AGG + chr2_1:18521280-18521299 Msa0199480:intron 30.0%
ATGATATTCTAACAACCTAC+AGG - chr2_1:18521389-18521408 None:intergenic 30.0%
GCTTATATTGTGACGTATAA+AGG + chr2_1:18521609-18521628 Msa0199480:CDS 30.0%
AGTTAGAGTTTCAGAATTGG+TGG - chr2_1:18519396-18519415 None:intergenic 35.0%
TGGAGTTAGAGTTTCAGAAT+TGG - chr2_1:18519399-18519418 None:intergenic 35.0%
AGAGGATATGATGTTACTGA+TGG - chr2_1:18519444-18519463 None:intergenic 35.0%
! ATCATCATAGCATGATGATG+TGG - chr2_1:18519474-18519493 None:intergenic 35.0%
! TGAAGTCATTTGGTTTAAGC+GGG - chr2_1:18519535-18519554 None:intergenic 35.0%
TATTATTAGTGATGTGGGTC+GGG - chr2_1:18519667-18519686 None:intergenic 35.0%
! AAAGGTGGAATTTCGATTTG+AGG - chr2_1:18519708-18519727 None:intergenic 35.0%
TGTTGGAAGATGAAAAAAGG+TGG - chr2_1:18519723-18519742 None:intergenic 35.0%
!! GAGTGTTGGAAGATGAAAAA+AGG - chr2_1:18519726-18519745 None:intergenic 35.0%
! TGTTGTTGTTCAATGAGTGT+TGG - chr2_1:18519740-18519759 None:intergenic 35.0%
GAGCATAACATCTTCTACTT+CGG - chr2_1:18519783-18519802 None:intergenic 35.0%
! TAATTGGGGTAATGATAGTG+GGG - chr2_1:18519859-18519878 None:intergenic 35.0%
GTAATTGGGGTAATGATAGT+GGG - chr2_1:18519860-18519879 None:intergenic 35.0%
TTGCTTCTTTGTGTATGTTG+TGG - chr2_1:18520148-18520167 None:intergenic 35.0%
!! CTAGTTTGGGTTTCTAGAAT+TGG - chr2_1:18520178-18520197 None:intergenic 35.0%
! TAGAAACCCAAACTAGTTCT+AGG + chr2_1:18520182-18520201 Msa0199480:CDS 35.0%
!! AGAAACCCAAACTAGTTCTA+GGG + chr2_1:18520183-18520202 Msa0199480:CDS 35.0%
GTAAAACCCTAGAACTAGTT+TGG - chr2_1:18520192-18520211 None:intergenic 35.0%
!! GATTGTTGCTGATTTGTGTT+TGG - chr2_1:18520214-18520233 None:intergenic 35.0%
CAAACCTCAGATCAAGAAAA+AGG + chr2_1:18520233-18520252 Msa0199480:CDS 35.0%
ATGAACTTTCAACAGACTCA+TGG + chr2_1:18520431-18520450 Msa0199480:intron 35.0%
TGAACTTTCAACAGACTCAT+GGG + chr2_1:18520432-18520451 Msa0199480:intron 35.0%
ATGTATGAAAACCTTGGATG+TGG - chr2_1:18520496-18520515 None:intergenic 35.0%
CATTGCAAATATTCCACCTT+TGG + chr2_1:18520706-18520725 Msa0199480:intron 35.0%
CACACATATATATGCCATGA+TGG + chr2_1:18520962-18520981 Msa0199480:intron 35.0%
AAGAAATATGCCACTCATTG+TGG + chr2_1:18521176-18521195 Msa0199480:intron 35.0%
AGAAATATGCCACTCATTGT+GGG + chr2_1:18521177-18521196 Msa0199480:intron 35.0%
TTCTTTGCAATCTTGGAAAG+CGG - chr2_1:18521311-18521330 None:intergenic 35.0%
!! CAGCATCATTTTGTTATGTG+AGG - chr2_1:18521685-18521704 None:intergenic 35.0%
TCTGACATAGCTGTTATGTT+TGG - chr2_1:18521822-18521841 None:intergenic 35.0%
! ATTGGTGGCAGAAGTAGTAA+AGG - chr2_1:18519381-18519400 None:intergenic 40.0%
!! TATGATGTTACTGATGGTGG+TGG - chr2_1:18519438-18519457 None:intergenic 40.0%
GGATATGATGTTACTGATGG+TGG - chr2_1:18519441-18519460 None:intergenic 40.0%
! TGATGATGTGGTACTTTGAG+AGG - chr2_1:18519462-18519481 None:intergenic 40.0%
! GAAGTCATTTGGTTTAAGCG+GGG - chr2_1:18519534-18519553 None:intergenic 40.0%
! AATTGTGGTGGGATTGGAAT+TGG - chr2_1:18519579-18519598 None:intergenic 40.0%
ATTATTAGTGATGTGGGTCG+GGG - chr2_1:18519666-18519685 None:intergenic 40.0%
!! AATTGGGGTAATGATAGTGG+GGG - chr2_1:18519858-18519877 None:intergenic 40.0%
GGTAATTGGGGTAATGATAG+TGG - chr2_1:18519861-18519880 None:intergenic 40.0%
! AGTCAAGGTGCTAATAGTGT+TGG - chr2_1:18519909-18519928 None:intergenic 40.0%
! AGTGTTCGTCATGATAGTGT+TGG - chr2_1:18519957-18519976 None:intergenic 40.0%
GTATGCACGATCAAAATGAG+CGG - chr2_1:18520110-18520129 None:intergenic 40.0%
!! TATGTTGTGGACTAGATGGA+TGG - chr2_1:18520135-18520154 None:intergenic 40.0%
!! TGTGTATGTTGTGGACTAGA+TGG - chr2_1:18520139-18520158 None:intergenic 40.0%
AAAGAATGCCAAGATGTCCT+TGG + chr2_1:18520390-18520409 Msa0199480:intron 40.0%
ATAACACACCAAGGACATCT+TGG - chr2_1:18520401-18520420 None:intergenic 40.0%
! TGTCCTTGGTGTGTTATATG+AGG + chr2_1:18520404-18520423 Msa0199480:intron 40.0%
! GTCCTTGGTGTGTTATATGA+GGG + chr2_1:18520405-18520424 Msa0199480:intron 40.0%
TTCCCTCATATAACACACCA+AGG - chr2_1:18520410-18520429 None:intergenic 40.0%
ATGGAAAAAGATACGGTGTG+TGG - chr2_1:18521270-18521289 None:intergenic 40.0%
TTCCATTGAGCAATTAAGGC+TGG + chr2_1:18521284-18521303 Msa0199480:intron 40.0%
TCTTTGCAATCTTGGAAAGC+GGG - chr2_1:18521310-18521329 None:intergenic 40.0%
AACAGTAGGAAAATACGGTG+TGG + chr2_1:18521359-18521378 Msa0199480:intron 40.0%
GACTTTAACCACCTCAATAG+TGG + chr2_1:18521855-18521874 Msa0199480:CDS 40.0%
! ATAAAGCACCACTATTGAGG+TGG - chr2_1:18521866-18521885 None:intergenic 40.0%
! GGAATAAAGCACCACTATTG+AGG - chr2_1:18521869-18521888 None:intergenic 40.0%
! AGGGATAATTGTGGTGGGAT+TGG - chr2_1:18519585-18519604 None:intergenic 45.0%
GGGTTAGGGATAATTGTGGT+GGG - chr2_1:18519590-18519609 None:intergenic 45.0%
!!! TTTTGGAATACGGGTGCTAG+CGG - chr2_1:18519612-18519631 None:intergenic 45.0%
! GGGGTGGGGATAATGATTTT+TGG - chr2_1:18519629-18519648 None:intergenic 45.0%
!! GATGGTGGGAATGTGAATAG+CGG - chr2_1:18519822-18519841 None:intergenic 45.0%
!! AGGTGGTGGTAGTGGTAATT+GGG - chr2_1:18519874-18519893 None:intergenic 45.0%
!! AAGGTGGTGGTAGTGGTAAT+TGG - chr2_1:18519875-18519894 None:intergenic 45.0%
!! AAGGTGCTAATAGTGTTGGC+GGG - chr2_1:18519905-18519924 None:intergenic 45.0%
! CAAGGTGCTAATAGTGTTGG+CGG - chr2_1:18519906-18519925 None:intergenic 45.0%
AGTAGAGATGGTAGGAGTAG+TGG - chr2_1:18519933-18519952 None:intergenic 45.0%
!! GATAGTGTTGGCAGTAGAGA+TGG - chr2_1:18519945-18519964 None:intergenic 45.0%
AGCGGTTGTGATTTCAATGC+AGG - chr2_1:18520092-18520111 None:intergenic 45.0%
TTTCAACAGACTCATGGGCA+TGG + chr2_1:18520437-18520456 Msa0199480:intron 45.0%
TCATGGGCATGGAGTAAACA+TGG + chr2_1:18520448-18520467 Msa0199480:intron 45.0%
TTAAAGGGTCTCCACATCCA+AGG + chr2_1:18520482-18520501 Msa0199480:intron 45.0%
CAGGGACAAAAAACCAAAGG+TGG - chr2_1:18520722-18520741 None:intergenic 45.0%
!!! CACCTTTGGTTTTTTGTCCC+TGG + chr2_1:18520720-18520739 Msa0199480:intron 45.0%
CACCAGGGACAAAAAACCAA+AGG - chr2_1:18520725-18520744 None:intergenic 45.0%
ATATGCCACTCATTGTGGGA+CGG + chr2_1:18521181-18521200 Msa0199480:intron 45.0%
TACGGTGTGTGGAAAGTTGT+TGG - chr2_1:18521259-18521278 None:intergenic 45.0%
! ACCTGAGAATTGCTAGTCTC+TGG - chr2_1:18521720-18521739 None:intergenic 45.0%
!! AATATTATTCATATTAAAAT+TGG - chr2_1:18520848-18520867 None:intergenic 5.0%
GGTGGCAGAAGTAGTAAAGG+TGG - chr2_1:18519378-18519397 None:intergenic 50.0%
GTGGTGGTTAGGATTTGAGG+TGG - chr2_1:18519419-18519438 None:intergenic 50.0%
!! GTGGTGGTGGTTAGGATTTG+AGG - chr2_1:18519422-18519441 None:intergenic 50.0%
!! TACTGATGGTGGTGGTGGTT+AGG - chr2_1:18519430-18519449 None:intergenic 50.0%
!! GATGTTACTGATGGTGGTGG+TGG - chr2_1:18519435-18519454 None:intergenic 50.0%
GGGGTTAGGGATAATTGTGG+TGG - chr2_1:18519591-18519610 None:intergenic 50.0%
AGCGGGGTTAGGGATAATTG+TGG - chr2_1:18519594-18519613 None:intergenic 50.0%
! TTTGGAATACGGGTGCTAGC+GGG - chr2_1:18519611-18519630 None:intergenic 50.0%
GAATGTGAATAGCGGTGGTG+GGG - chr2_1:18519814-18519833 None:intergenic 50.0%
GGAATGTGAATAGCGGTGGT+GGG - chr2_1:18519815-18519834 None:intergenic 50.0%
GTGGTGAGAGTAGTGATGGT+GGG - chr2_1:18519836-18519855 None:intergenic 50.0%
!! GGTGGTGGTAGTGGTAATTG+GGG - chr2_1:18519873-18519892 None:intergenic 50.0%
!! AGGTGCTAATAGTGTTGGCG+GGG - chr2_1:18519904-18519923 None:intergenic 50.0%
! GTGTTGGCAGTAGAGATGGT+AGG - chr2_1:18519941-18519960 None:intergenic 50.0%
GTGGTGTGGGTGTGTGTATT+AGG - chr2_1:18520580-18520599 None:intergenic 50.0%
GGCCAGCCTTAATTGCTCAA+TGG - chr2_1:18521289-18521308 None:intergenic 50.0%
ATACGGTGTGGACAACCTGT+AGG + chr2_1:18521371-18521390 Msa0199480:intron 50.0%
GCCAGAGACTAGCAATTCTC+AGG + chr2_1:18521716-18521735 Msa0199480:CDS 50.0%
TTGGAATACGGGTGCTAGCG+GGG - chr2_1:18519610-18519629 None:intergenic 55.0%
ATTAGTGATGTGGGTCGGGG+TGG - chr2_1:18519663-18519682 None:intergenic 55.0%
GGGAATGTGAATAGCGGTGG+TGG - chr2_1:18519816-18519835 None:intergenic 55.0%
GGTGGGAATGTGAATAGCGG+TGG - chr2_1:18519819-18519838 None:intergenic 55.0%
GGTGGTGAGAGTAGTGATGG+TGG - chr2_1:18519837-18519856 None:intergenic 55.0%
!! TGGGGTAATGATAGTGGGGG+TGG - chr2_1:18519855-18519874 None:intergenic 55.0%
! GTTAGCGAAGGTGGTGGTAG+TGG - chr2_1:18519882-18519901 None:intergenic 55.0%
GGTAGGAGTAGTGGCAGTCA+AGG - chr2_1:18519924-18519943 None:intergenic 55.0%
GTAATGTGTGCGCGCGCAAA+TGG + chr2_1:18521444-18521463 Msa0199480:intron 55.0%
TACGGGTGCTAGCGGGGTTA+GGG - chr2_1:18519604-18519623 None:intergenic 60.0%
ATACGGGTGCTAGCGGGGTT+AGG - chr2_1:18519605-18519624 None:intergenic 60.0%
!! TGGTGGTGGTTGAAGGGGTG+GGG - chr2_1:18519643-18519662 None:intergenic 60.0%
!! GTGGTGGTGGTTGAAGGGGT+GGG - chr2_1:18519644-18519663 None:intergenic 60.0%
!! TCGGGGTGGTGGTGGTTGAA+GGG - chr2_1:18519649-18519668 None:intergenic 60.0%
GGGGGTGGTGAGAGTAGTGA+TGG - chr2_1:18519840-18519859 None:intergenic 60.0%
!! AGTGTTGGCGGGGTTAGCGA+AGG - chr2_1:18519894-18519913 None:intergenic 60.0%
CGGTTCCGTCCCACAATGAG+TGG - chr2_1:18521189-18521208 None:intergenic 60.0%
!! GGTGGTGGTGGTTGAAGGGG+TGG - chr2_1:18519645-18519664 None:intergenic 65.0%
!! CGGGGTGGTGGTGGTTGAAG+GGG - chr2_1:18519648-18519667 None:intergenic 65.0%
!! GTCGGGGTGGTGGTGGTTGA+AGG - chr2_1:18519650-18519669 None:intergenic 65.0%
AGTGATGTGGGTCGGGGTGG+TGG - chr2_1:18519660-18519679 None:intergenic 65.0%
! GTTGGCGGGGTTAGCGAAGG+TGG - chr2_1:18519891-18519910 None:intergenic 65.0%
! GATGTGGGTCGGGGTGGTGG+TGG - chr2_1:18519657-18519676 None:intergenic 70.0%
! GGCGGGGTTAGCGAAGGTGG+TGG - chr2_1:18519888-18519907 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 18519323 18521893 18519323 ID=Msa0199480;Name=Msa0199480
chr2_1 mRNA 18519323 18521893 18519323 ID=Msa0199480-mRNA-1;Parent=Msa0199480;Name=Msa0199480-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0|0|1|1|1|2|0|430
chr2_1 exon 18519323 18520254 18519323 ID=Msa0199480-mRNA-1:exon:7800;Parent=Msa0199480-mRNA-1
chr2_1 exon 18521533 18521893 18521533 ID=Msa0199480-mRNA-1:exon:7801;Parent=Msa0199480-mRNA-1
chr2_1 CDS 18519323 18520254 18519323 ID=Msa0199480-mRNA-1:cds;Parent=Msa0199480-mRNA-1
chr2_1 CDS 18521533 18521893 18521533 ID=Msa0199480-mRNA-1:cds;Parent=Msa0199480-mRNA-1
Gene Sequence

>Msa0199480

ATGGATAATGATGATTGGGATCTCTTTTCTATTGTGCGTAGCTGCAACACTACCACCTTTACTACTTCTGCCACCAATTCTGAAACTCTAACTCCACCTCAAATCCTAACCACCACCACCATCAGTAACATCATATCCTCTCAAAGTACCACATCATCATGCTATGATGATTTTACTTTTATCCAAGAAAATAATGCAATCTCTTTTGCCCCGCTTAAACCAAATGACTTCATAGAGTTAGATAAATTGATGACCAATTCCAATCCCACCACAATTATCCCTAACCCCGCTAGCACCCGTATTCCAAAAATCATTATCCCCACCCCTTCAACCACCACCACCCCGACCCACATCACTAATAATATTAACACTAGTGTTCATGCCTCAAATCGAAATTCCACCTTTTTTCATCTTCCAACACTCATTGAACAACAACAAATGCAACGAATTGAGTTCACCGAAGTAGAAGATGTTATGCTCAAATTCAACCCCACCACCGCTATTCACATTCCCACCATCACTACTCTCACCACCCCCACTATCATTACCCCAATTACCACTACCACCACCTTCGCTAACCCCGCCAACACTATTAGCACCTTGACTGCCACTACTCCTACCATCTCTACTGCCAACACTATCATGACGAACACTAGCGTTCATGAAACCAATCAATATCCTAGTGTTAGTGATTTTTCAACACTCGTTGAACAACAACATATTCATCAAAGCAACAACAATCAAGTGTTTGTTTCGAAACATGTTGCCTGCATTGAAATCACAACCGCTCATTTTGATCGTGCATACAGCCATCCATCTAGTCCACAACATACACAAAGAAGCAATCAACTTCCAATTCTAGAAACCCAAACTAGTTCTAGGGTTTTACCAAACACAAATCAGCAACAATCAAACCTCAGATCAAGAAAAAGGTGCTATTATAAGTGCAGCAGTTTCGCAAATTGTCCAGCAAAAAAATATGTAGAGAAAAGCAAAACTCATGAGAACGCTTATATTGTGACGTATAAAGGAGAGCATGATCACCAAAAACCAGAAGAAATTAAGAACTTTGTTGTTGGTACCTCACATAACAAAATGATGCTGAAGTTTGATGAGCCAGAGACTAGCAATTCTCAGGTTTTTTCCGATGAATCAAAAATCTCTTGTTCTGAAACAGAATTTATTGGAAACTCTAATGATGATGATGACATTTTGATACCAAACATAACAGCTATGTCAGAAGATTTATTGATGGACTTTAACCACCTCAATAGTGGTGCTTTATTCCCATAG
Protein sequence

>Msa0199480

MDNDDWDLFSIVRSCNTTTFTTSATNSETLTPPQILTTTTISNIISSQSTTSSCYDDFTFIQENNAISFAPLKPNDFIELDKLMTNSNPTTIIPNPASTRIPKIIIPTPSTTTTPTHITNNINTSVHASNRNSTFFHLPTLIEQQQMQRIEFTEVEDVMLKFNPTTAIHIPTITTLTTPTIITPITTTTTFANPANTISTLTATTPTISTANTIMTNTSVHETNQYPSVSDFSTLVEQQHIHQSNNNQVFVSKHVACIEITTAHFDRAYSHPSSPQHTQRSNQLPILETQTSSRVLPNTNQQQSNLRSRKRCYYKCSSFANCPAKKYVEKSKTHENAYIVTYKGEHDHQKPEEIKNFVVGTSHNKMMLKFDEPETSNSQVFSDESKISCSETEFIGNSNDDDDILIPNITAMSEDLLMDFNHLNSGALFP*