AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0202990


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0202990 MtrunA17_Chr2g0300511 97.541 122 3 0 1 122 1 122 1.92e-87 259
Msa0202990 MtrunA17_Chr4g0070591 82.540 126 18 2 3 128 5 126 4.81e-72 220
Msa0202990 MtrunA17_Chr8g0343881 68.908 119 33 2 2 120 6 120 5.90e-58 183
Msa0202990 MtrunA17_Chr4g0021261 62.016 129 31 4 7 130 25 140 5.78e-47 154
Msa0202990 MtrunA17_Chr1g0152191 55.833 120 53 0 7 126 144 263 3.23e-42 147
Msa0202990 MtrunA17_Chr5g0409471 60.177 113 29 2 7 119 16 112 2.88e-41 139
Msa0202990 MtrunA17_Chr7g0265931 54.688 128 43 4 2 120 66 187 6.94e-36 127
Msa0202990 MtrunA17_Chr3g0127641 46.610 118 41 3 7 124 112 207 1.50e-28 108
Msa0202990 MtrunA17_Chr8g0351521 43.697 119 45 3 3 119 50 148 1.27e-23 94.7
Msa0202990 MtrunA17_Chr8g0351521 66.667 39 13 0 75 113 302 340 3.02e-13 65.5
Msa0202990 MtrunA17_Chr8g0351291 56.522 46 20 0 75 120 51 96 7.30e-13 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0202990 AT3G13960.1 71.681 113 28 2 7 119 16 124 5.27e-56 180
Msa0202990 AT2G06200.1 64.167 120 36 2 5 117 4 123 3.39e-48 155
Msa0202990 AT2G06200.2 63.636 121 37 2 4 117 42 162 3.75e-48 156
Msa0202990 AT4G37740.1 51.908 131 55 4 5 134 162 285 8.96e-40 140
Msa0202990 AT2G36400.1 58.407 113 44 2 8 119 77 187 4.98e-36 128
Msa0202990 AT3G52910.1 55.172 116 44 4 8 119 83 194 3.78e-34 122
Msa0202990 AT2G22840.1 56.034 116 43 4 5 119 131 239 4.96e-34 124
Msa0202990 AT2G06200.3 92.157 51 2 1 69 117 1 51 4.57e-27 99.8
Msa0202990 AT5G53660.1 49.123 114 37 3 6 119 58 150 8.75e-27 103
Msa0202990 AT4G24150.3 43.200 125 60 3 1 119 104 223 1.12e-24 98.2
Msa0202990 AT4G24150.1 41.007 139 53 3 8 119 150 286 1.24e-24 98.2
Msa0202990 AT4G24150.2 43.200 125 60 3 1 119 142 261 1.26e-24 98.2
Msa0202990 AT2G45480.2 39.669 121 56 3 3 116 19 129 1.24e-18 80.5
Msa0202990 AT2G45480.3 39.669 121 56 3 3 116 19 129 5.66e-18 79.3
Msa0202990 AT2G45480.3 60.417 48 18 1 72 119 303 349 9.08e-14 67.4
Msa0202990 AT2G45480.1 39.669 121 56 3 3 116 17 127 5.80e-18 79.3
Msa0202990 AT2G45480.1 60.417 48 18 1 72 119 301 347 9.15e-14 67.4

Find 26 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCCATTGTGATGGAGTAAA+AGG 0.243712 2_1:+26148421 None:intergenic
CTTTCACAATATTTAGAATC+TGG 0.287060 2_1:+26147516 None:intergenic
ATTTACAAGTATATGGCTTC+TGG 0.291564 2_1:-26148376 Msa0202990:CDS
AGCTCTTATTTACAAGTATA+TGG 0.299426 2_1:-26148383 Msa0202990:CDS
AACTATCTGCAAATGGGTTT+AGG 0.375973 2_1:-26147615 Msa0202990:CDS
TGCAAGGAGAATGAAGTTAC+TGG 0.391304 2_1:-26147270 Msa0202990:CDS
GAAGAACAGATGGAAAGAAA+TGG 0.403362 2_1:-26147560 Msa0202990:CDS
CTTCTACATCTACCTGGCTC+TGG 0.445022 2_1:+26147579 None:intergenic
TGGATGGAACTATCTGCAAA+TGG 0.447963 2_1:-26147622 Msa0202990:CDS
TTGGTAAGGTAGACGCTGAT+AGG 0.460291 2_1:+26147295 None:intergenic
ATTCTCCTTGCACCTTGGTA+AGG 0.465903 2_1:+26147281 None:intergenic
CAGTGTTGGTGTTGTTGAGT+AGG 0.494839 2_1:+26148277 None:intergenic
CTCTCGGTAGTTCTACAAAC+GGG 0.499015 2_1:-26147322 Msa0202990:intron
GGATGGAACTATCTGCAAAT+GGG 0.512539 2_1:-26147621 Msa0202990:CDS
TGGTAAGGTAGACGCTGATA+GGG 0.539865 2_1:+26147296 None:intergenic
CCTCTCGGTAGTTCTACAAA+CGG 0.553923 2_1:-26147323 Msa0202990:intron
GGTAGATGTAGAAGAACAGA+TGG 0.565829 2_1:-26147570 Msa0202990:CDS
TCAAGTTCTTGCCATTGTGA+TGG 0.567945 2_1:+26148412 None:intergenic
ACTTCATTCTCCTTGCACCT+TGG 0.574225 2_1:+26147276 None:intergenic
TCTGTTCTTCTACATCTACC+TGG 0.588963 2_1:+26147573 None:intergenic
TGTGAAAGACACATGCATAG+AGG 0.590712 2_1:-26147501 Msa0202990:CDS
GCAAGGAGAATGAAGTTACT+GGG 0.596245 2_1:-26147269 Msa0202990:CDS
GTCTACCTTACCAAGGTGCA+AGG 0.607221 2_1:-26147286 Msa0202990:CDS
AGAGAAAGGACTTACAGTGT+TGG 0.629687 2_1:+26148263 None:intergenic
AAACCGTTCAAGAAAGCCTG+TGG 0.641388 2_1:-26147475 Msa0202990:CDS
TATCAGCGTCTACCTTACCA+AGG 0.719838 2_1:-26147293 Msa0202990:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAGAGATAATTTAGTTAA+AGG - chr2_1:26147983-26148002 Msa0202990:intron 15.0%
!!! TTATTTTGATTTTTTTCAGT+TGG - chr2_1:26148057-26148076 Msa0202990:intron 15.0%
!! TATAGAGATAATTTAGTTAA+AGG - chr2_1:26147983-26148002 Msa0202990:intron 15.0%
!!! TTATTTTGATTTTTTTCAGT+TGG - chr2_1:26148057-26148076 Msa0202990:intron 15.0%
!!! AGTATGTTTTAAACTCTTAA+CGG + chr2_1:26147479-26147498 None:intergenic 20.0%
!!! TTTAAAGCAAATTTAGAAAG+TGG - chr2_1:26147645-26147664 Msa0202990:intron 20.0%
!!! TATATTATATCAAAGGTGTT+AGG + chr2_1:26147767-26147786 None:intergenic 20.0%
!! GAAGATGTATATTATATCAA+AGG + chr2_1:26147774-26147793 None:intergenic 20.0%
!!! CTATACATTCATAATCTTTT+AGG + chr2_1:26147968-26147987 None:intergenic 20.0%
!! AAAAAATCAAAATAACCCTT+TGG + chr2_1:26148052-26148071 None:intergenic 20.0%
!!! AGTATGTTTTAAACTCTTAA+CGG + chr2_1:26147479-26147498 None:intergenic 20.0%
!!! TTTAAAGCAAATTTAGAAAG+TGG - chr2_1:26147645-26147664 Msa0202990:intron 20.0%
!!! TATATTATATCAAAGGTGTT+AGG + chr2_1:26147767-26147786 None:intergenic 20.0%
!! GAAGATGTATATTATATCAA+AGG + chr2_1:26147774-26147793 None:intergenic 20.0%
!!! CTATACATTCATAATCTTTT+AGG + chr2_1:26147968-26147987 None:intergenic 20.0%
!! AAAAAATCAAAATAACCCTT+TGG + chr2_1:26148052-26148071 None:intergenic 20.0%
!! AGCTCTTATTTACAAGTATA+TGG - chr2_1:26147316-26147335 Msa0202990:intron 25.0%
! ACATATGAATATAGAGAGAA+AGG + chr2_1:26147453-26147472 None:intergenic 25.0%
!!! TTTGATTTTTTTCAGTTGGA+TGG - chr2_1:26148061-26148080 Msa0202990:intron 25.0%
!! CTTTCACAATATTTAGAATC+TGG + chr2_1:26148186-26148205 None:intergenic 25.0%
!! AGCTCTTATTTACAAGTATA+TGG - chr2_1:26147316-26147335 Msa0202990:intron 25.0%
! ACATATGAATATAGAGAGAA+AGG + chr2_1:26147453-26147472 None:intergenic 25.0%
!!! TTTGATTTTTTTCAGTTGGA+TGG - chr2_1:26148061-26148080 Msa0202990:intron 25.0%
!! CTTTCACAATATTTAGAATC+TGG + chr2_1:26148186-26148205 None:intergenic 25.0%
ATTTACAAGTATATGGCTTC+TGG - chr2_1:26147323-26147342 Msa0202990:intron 30.0%
TACCATCAGAAAAAGCTATT+TGG - chr2_1:26147373-26147392 Msa0202990:intron 30.0%
!! GTATTTTTGTGTGTTGCAAA+CGG + chr2_1:26147813-26147832 None:intergenic 30.0%
TTAGCAAAGAAGCTAGTATT+TGG + chr2_1:26147881-26147900 None:intergenic 30.0%
ATTTACAAGTATATGGCTTC+TGG - chr2_1:26147323-26147342 Msa0202990:intron 30.0%
TACCATCAGAAAAAGCTATT+TGG - chr2_1:26147373-26147392 Msa0202990:intron 30.0%
!! GTATTTTTGTGTGTTGCAAA+CGG + chr2_1:26147813-26147832 None:intergenic 30.0%
TTAGCAAAGAAGCTAGTATT+TGG + chr2_1:26147881-26147900 None:intergenic 30.0%
! TTCCTTTTACTCCATCACAA+TGG - chr2_1:26147276-26147295 Msa0202990:CDS 35.0%
ATGGTAAAAAGAAGATCAGG+TGG + chr2_1:26147359-26147378 None:intergenic 35.0%
CTGATGGTAAAAAGAAGATC+AGG + chr2_1:26147362-26147381 None:intergenic 35.0%
!! GTCCAAATAGCTTTTTCTGA+TGG + chr2_1:26147378-26147397 None:intergenic 35.0%
GGAAAAAGTCTTGAAGAAAG+AGG + chr2_1:26147404-26147423 None:intergenic 35.0%
TATACATCTTCCTCTGTGTT+TGG - chr2_1:26147783-26147802 Msa0202990:intron 35.0%
TGCAACACACAAAAATACGT+TGG - chr2_1:26147815-26147834 Msa0202990:intron 35.0%
AAATGAGTCTCTTTGACCAA+AGG - chr2_1:26148033-26148052 Msa0202990:intron 35.0%
AATGAGTCTCTTTGACCAAA+GGG - chr2_1:26148034-26148053 Msa0202990:intron 35.0%
! AACTATCTGCAAATGGGTTT+AGG - chr2_1:26148084-26148103 Msa0202990:intron 35.0%
GAAGAACAGATGGAAAGAAA+TGG - chr2_1:26148139-26148158 Msa0202990:intron 35.0%
! TTCCTTTTACTCCATCACAA+TGG - chr2_1:26147276-26147295 Msa0202990:CDS 35.0%
ATGGTAAAAAGAAGATCAGG+TGG + chr2_1:26147359-26147378 None:intergenic 35.0%
CTGATGGTAAAAAGAAGATC+AGG + chr2_1:26147362-26147381 None:intergenic 35.0%
!! GTCCAAATAGCTTTTTCTGA+TGG + chr2_1:26147378-26147397 None:intergenic 35.0%
GGAAAAAGTCTTGAAGAAAG+AGG + chr2_1:26147404-26147423 None:intergenic 35.0%
TATACATCTTCCTCTGTGTT+TGG - chr2_1:26147783-26147802 Msa0202990:intron 35.0%
TGCAACACACAAAAATACGT+TGG - chr2_1:26147815-26147834 Msa0202990:intron 35.0%
AAATGAGTCTCTTTGACCAA+AGG - chr2_1:26148033-26148052 Msa0202990:intron 35.0%
AATGAGTCTCTTTGACCAAA+GGG - chr2_1:26148034-26148053 Msa0202990:intron 35.0%
! AACTATCTGCAAATGGGTTT+AGG - chr2_1:26148084-26148103 Msa0202990:intron 35.0%
GAAGAACAGATGGAAAGAAA+TGG - chr2_1:26148139-26148158 Msa0202990:intron 35.0%
! TGCCATTGTGATGGAGTAAA+AGG + chr2_1:26147281-26147300 None:intergenic 40.0%
TCAAGTTCTTGCCATTGTGA+TGG + chr2_1:26147290-26147309 None:intergenic 40.0%
AGAGAAAGGACTTACAGTGT+TGG + chr2_1:26147439-26147458 None:intergenic 40.0%
!! TATCAAAGGTGTTAGGTCAG+TGG + chr2_1:26147760-26147779 None:intergenic 40.0%
AAACGGATAACCAAACACAG+AGG + chr2_1:26147796-26147815 None:intergenic 40.0%
! TACTAGCTTCTTTGCTAACG+TGG - chr2_1:26147883-26147902 Msa0202990:intron 40.0%
GTTTGCAAAACGGTGTTTGA+TGG + chr2_1:26147925-26147944 None:intergenic 40.0%
TTCGTACAGCGTTTGCAAAA+CGG + chr2_1:26147935-26147954 None:intergenic 40.0%
TGGATGGAACTATCTGCAAA+TGG - chr2_1:26148077-26148096 Msa0202990:intron 40.0%
GGATGGAACTATCTGCAAAT+GGG - chr2_1:26148078-26148097 Msa0202990:intron 40.0%
AGAAAAATAGATCCAGAGCC+AGG - chr2_1:26148108-26148127 Msa0202990:intron 40.0%
TCTGTTCTTCTACATCTACC+TGG + chr2_1:26148129-26148148 None:intergenic 40.0%
GGTAGATGTAGAAGAACAGA+TGG - chr2_1:26148129-26148148 Msa0202990:intron 40.0%
TGTGAAAGACACATGCATAG+AGG - chr2_1:26148198-26148217 Msa0202990:intron 40.0%
TGCAAGGAGAATGAAGTTAC+TGG - chr2_1:26148429-26148448 Msa0202990:CDS 40.0%
GCAAGGAGAATGAAGTTACT+GGG - chr2_1:26148430-26148449 Msa0202990:CDS 40.0%
! TGCCATTGTGATGGAGTAAA+AGG + chr2_1:26147281-26147300 None:intergenic 40.0%
TCAAGTTCTTGCCATTGTGA+TGG + chr2_1:26147290-26147309 None:intergenic 40.0%
AGAGAAAGGACTTACAGTGT+TGG + chr2_1:26147439-26147458 None:intergenic 40.0%
!! TATCAAAGGTGTTAGGTCAG+TGG + chr2_1:26147760-26147779 None:intergenic 40.0%
AAACGGATAACCAAACACAG+AGG + chr2_1:26147796-26147815 None:intergenic 40.0%
! TACTAGCTTCTTTGCTAACG+TGG - chr2_1:26147883-26147902 Msa0202990:intron 40.0%
GTTTGCAAAACGGTGTTTGA+TGG + chr2_1:26147925-26147944 None:intergenic 40.0%
TTCGTACAGCGTTTGCAAAA+CGG + chr2_1:26147935-26147954 None:intergenic 40.0%
TGGATGGAACTATCTGCAAA+TGG - chr2_1:26148077-26148096 Msa0202990:intron 40.0%
GGATGGAACTATCTGCAAAT+GGG - chr2_1:26148078-26148097 Msa0202990:intron 40.0%
AGAAAAATAGATCCAGAGCC+AGG - chr2_1:26148108-26148127 Msa0202990:intron 40.0%
TCTGTTCTTCTACATCTACC+TGG + chr2_1:26148129-26148148 None:intergenic 40.0%
GGTAGATGTAGAAGAACAGA+TGG - chr2_1:26148129-26148148 Msa0202990:intron 40.0%
TGTGAAAGACACATGCATAG+AGG - chr2_1:26148198-26148217 Msa0202990:intron 40.0%
TGCAAGGAGAATGAAGTTAC+TGG - chr2_1:26148429-26148448 Msa0202990:CDS 40.0%
GCAAGGAGAATGAAGTTACT+GGG - chr2_1:26148430-26148449 Msa0202990:CDS 40.0%
!! CAGTGTTGGTGTTGTTGAGT+AGG + chr2_1:26147425-26147444 None:intergenic 45.0%
AAACCGTTCAAGAAAGCCTG+TGG - chr2_1:26148224-26148243 Msa0202990:intron 45.0%
CTTCCACAGGCTTTCTTGAA+CGG + chr2_1:26148230-26148249 None:intergenic 45.0%
CGTTTGTAGAACTACCGAGA+GGG + chr2_1:26148378-26148397 None:intergenic 45.0%
! CCTCTCGGTAGTTCTACAAA+CGG - chr2_1:26148376-26148395 Msa0202990:CDS 45.0%
! CTCTCGGTAGTTCTACAAAC+GGG - chr2_1:26148377-26148396 Msa0202990:CDS 45.0%
TGGTAAGGTAGACGCTGATA+GGG + chr2_1:26148406-26148425 None:intergenic 45.0%
TTGGTAAGGTAGACGCTGAT+AGG + chr2_1:26148407-26148426 None:intergenic 45.0%
TATCAGCGTCTACCTTACCA+AGG - chr2_1:26148406-26148425 Msa0202990:CDS 45.0%
ATTCTCCTTGCACCTTGGTA+AGG + chr2_1:26148421-26148440 None:intergenic 45.0%
ACTTCATTCTCCTTGCACCT+TGG + chr2_1:26148426-26148445 None:intergenic 45.0%
!! CAGTGTTGGTGTTGTTGAGT+AGG + chr2_1:26147425-26147444 None:intergenic 45.0%
AAACCGTTCAAGAAAGCCTG+TGG - chr2_1:26148224-26148243 Msa0202990:intron 45.0%
CTTCCACAGGCTTTCTTGAA+CGG + chr2_1:26148230-26148249 None:intergenic 45.0%
CGTTTGTAGAACTACCGAGA+GGG + chr2_1:26148378-26148397 None:intergenic 45.0%
! CCTCTCGGTAGTTCTACAAA+CGG - chr2_1:26148376-26148395 Msa0202990:CDS 45.0%
! CTCTCGGTAGTTCTACAAAC+GGG - chr2_1:26148377-26148396 Msa0202990:CDS 45.0%
TGGTAAGGTAGACGCTGATA+GGG + chr2_1:26148406-26148425 None:intergenic 45.0%
TTGGTAAGGTAGACGCTGAT+AGG + chr2_1:26148407-26148426 None:intergenic 45.0%
TATCAGCGTCTACCTTACCA+AGG - chr2_1:26148406-26148425 Msa0202990:CDS 45.0%
ATTCTCCTTGCACCTTGGTA+AGG + chr2_1:26148421-26148440 None:intergenic 45.0%
ACTTCATTCTCCTTGCACCT+TGG + chr2_1:26148426-26148445 None:intergenic 45.0%
CTTCTACATCTACCTGGCTC+TGG + chr2_1:26148123-26148142 None:intergenic 50.0%
ACACTGCACAATCTCCCTCT+CGG - chr2_1:26148361-26148380 Msa0202990:CDS 50.0%
CCGTTTGTAGAACTACCGAG+AGG + chr2_1:26148379-26148398 None:intergenic 50.0%
GTCTACCTTACCAAGGTGCA+AGG - chr2_1:26148413-26148432 Msa0202990:CDS 50.0%
CTTCTACATCTACCTGGCTC+TGG + chr2_1:26148123-26148142 None:intergenic 50.0%
ACACTGCACAATCTCCCTCT+CGG - chr2_1:26148361-26148380 Msa0202990:CDS 50.0%
CCGTTTGTAGAACTACCGAG+AGG + chr2_1:26148379-26148398 None:intergenic 50.0%
GTCTACCTTACCAAGGTGCA+AGG - chr2_1:26148413-26148432 Msa0202990:CDS 50.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 26147260 26148461 26147260 ID=Msa0202990;Name=Msa0202990
chr2_1 mRNA 26147260 26148461 26147260 ID=Msa0202990-mRNA-1;Parent=Msa0202990;Name=Msa0202990-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|0.5|0|0.66|1|1|3|0|143
chr2_1 exon 26148278 26148461 26148278 ID=Msa0202990-mRNA-1:exon:9864;Parent=Msa0202990-mRNA-1
chr2_1 exon 26147473 26147645 26147473 ID=Msa0202990-mRNA-1:exon:9863;Parent=Msa0202990-mRNA-1
chr2_1 exon 26147260 26147334 26147260 ID=Msa0202990-mRNA-1:exon:9862;Parent=Msa0202990-mRNA-1
chr2_1 CDS 26148278 26148461 26148278 ID=Msa0202990-mRNA-1:cds;Parent=Msa0202990-mRNA-1
chr2_1 CDS 26147473 26147645 26147473 ID=Msa0202990-mRNA-1:cds;Parent=Msa0202990-mRNA-1
chr2_1 CDS 26147260 26147334 26147260 ID=Msa0202990-mRNA-1:cds;Parent=Msa0202990-mRNA-1
Gene Sequence

>Msa0202990

ATGAACAAGAACAAGTTTCCTTTTACTCCATCACAATGGCAAGAACTTGAACATCAAGCTCTTATTTACAAGTATATGGCTTCTGGTATATCTATTCCACCTGATCTTCTTTTTACCATCAGAAAAAGCTATTTGGACTCTCCTCTTTCTTCAAGACTTTTTCCTACTCAACAACACCAACACTTTGGATGGAACTATCTGCAAATGGGTTTAGGAAGAAAAATAGATCCAGAGCCAGGTAGATGTAGAAGAACAGATGGAAAGAAATGGAGATGTTCAAAAGAAGCATATCCAGATTCTAAATATTGTGAAAGACACATGCATAGAGGAAAAAACCGTTCAAGAAAGCCTGTGGAATTCTACAAACGGGAAACCCTATCAGCGTCTACCTTACCAAGGTGCAAGGAGAATGAAGTTACTGGGCTGATTTAA

Protein sequence

>Msa0202990

MNKNKFPFTPSQWQELEHQALIYKYMASGISIPPDLLFTIRKSYLDSPLSSRLFPTQQHQHFGWNYLQMGLGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVEFYKRETLSASTLPRCKENEVTGLI*