AlfalfaGEDB | Msa0202990

M. sativa cultivar zhongmu-4 / Msa0202990

KEGG: -
GO: -
PFAM: PF08880.14

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0202990	KEH37559.1	97.541	122	3	0	1	122	1	122	1.22e-83	259

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0202990	sp\|Q8L8A6\|GRF5_ARATH	71.681	113	28	2	7	119	16	124	5.18e-55	180

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0202990	A0A072V671	97.541	122	3	0	1	122	1	122	5.84e-84	259

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TFs/TRs:

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Gene ID	Type	Classification
Msa0202990	TF	GRF

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0202990	MtrunA17_Chr2g0300511	97.541	122	3	0	1	122	1	122	1.92e-87	259
Msa0202990	MtrunA17_Chr4g0070591	82.540	126	18	2	3	128	5	126	4.81e-72	220
Msa0202990	MtrunA17_Chr8g0343881	68.908	119	33	2	2	120	6	120	5.90e-58	183
Msa0202990	MtrunA17_Chr4g0021261	62.016	129	31	4	7	130	25	140	5.78e-47	154
Msa0202990	MtrunA17_Chr1g0152191	55.833	120	53	0	7	126	144	263	3.23e-42	147
Msa0202990	MtrunA17_Chr5g0409471	60.177	113	29	2	7	119	16	112	2.88e-41	139
Msa0202990	MtrunA17_Chr7g0265931	54.688	128	43	4	2	120	66	187	6.94e-36	127
Msa0202990	MtrunA17_Chr3g0127641	46.610	118	41	3	7	124	112	207	1.50e-28	108
Msa0202990	MtrunA17_Chr8g0351521	43.697	119	45	3	3	119	50	148	1.27e-23	94.7
Msa0202990	MtrunA17_Chr8g0351521	66.667	39	13	0	75	113	302	340	3.02e-13	65.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0202990	AT3G13960.1	71.681	113	28	2	7	119	16	124	5.27e-56	180
Msa0202990	AT2G06200.1	64.167	120	36	2	5	117	4	123	3.39e-48	155
Msa0202990	AT2G06200.2	63.636	121	37	2	4	117	42	162	3.75e-48	156
Msa0202990	AT4G37740.1	51.908	131	55	4	5	134	162	285	8.96e-40	140
Msa0202990	AT2G36400.1	58.407	113	44	2	8	119	77	187	4.98e-36	128
Msa0202990	AT3G52910.1	55.172	116	44	4	8	119	83	194	3.78e-34	122
Msa0202990	AT2G22840.1	56.034	116	43	4	5	119	131	239	4.96e-34	124
Msa0202990	AT2G06200.3	92.157	51	2	1	69	117	1	51	4.57e-27	99.8
Msa0202990	AT5G53660.1	49.123	114	37	3	6	119	58	150	8.75e-27	103
Msa0202990	AT4G24150.3	43.200	125	60	3	1	119	104	223	1.12e-24	98.2

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Find 26 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCGTTCAAGAAAGCCTG+TGG	0.641388	2_1:-26147475	Msa0202990:CDS
AACTATCTGCAAATGGGTTT+AGG	0.375973	2_1:-26147615	Msa0202990:CDS
ACTTCATTCTCCTTGCACCT+TGG	0.574225	2_1:+26147276	None:intergenic
AGAGAAAGGACTTACAGTGT+TGG	0.629687	2_1:+26148263	None:intergenic
AGCTCTTATTTACAAGTATA+TGG	0.299426	2_1:-26148383	Msa0202990:CDS
ATTCTCCTTGCACCTTGGTA+AGG	0.465903	2_1:+26147281	None:intergenic
ATTTACAAGTATATGGCTTC+TGG	0.291564	2_1:-26148376	Msa0202990:CDS
CAGTGTTGGTGTTGTTGAGT+AGG	0.494839	2_1:+26148277	None:intergenic
CCTCTCGGTAGTTCTACAAA+CGG	0.553923	2_1:-26147323	Msa0202990:intron
CTCTCGGTAGTTCTACAAAC+GGG	0.499015	2_1:-26147322	Msa0202990:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTTACAAGTATATGGCTTC+TGG	-	chr2_1:26147323-26147342	Msa0202990:intron	30.0%
TACCATCAGAAAAAGCTATT+TGG	-	chr2_1:26147373-26147392	Msa0202990:intron	30.0%
TTAGCAAAGAAGCTAGTATT+TGG	+	chr2_1:26147881-26147900	None:intergenic	30.0%
ATTTACAAGTATATGGCTTC+TGG	-	chr2_1:26147323-26147342	Msa0202990:intron	30.0%
TACCATCAGAAAAAGCTATT+TGG	-	chr2_1:26147373-26147392	Msa0202990:intron	30.0%
TTAGCAAAGAAGCTAGTATT+TGG	+	chr2_1:26147881-26147900	None:intergenic	30.0%
ATGGTAAAAAGAAGATCAGG+TGG	+	chr2_1:26147359-26147378	None:intergenic	35.0%
CTGATGGTAAAAAGAAGATC+AGG	+	chr2_1:26147362-26147381	None:intergenic	35.0%
GGAAAAAGTCTTGAAGAAAG+AGG	+	chr2_1:26147404-26147423	None:intergenic	35.0%
TATACATCTTCCTCTGTGTT+TGG	-	chr2_1:26147783-26147802	Msa0202990:intron	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	26147260	26148461	26147260	ID=Msa0202990;Name=Msa0202990
chr2_1	mRNA	26147260	26148461	26147260	ID=Msa0202990-mRNA-1;Parent=Msa0202990;Name=Msa0202990-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0\|0.5\|0\|0.66\|1\|1\|3\|0\|143
chr2_1	exon	26148278	26148461	26148278	ID=Msa0202990-mRNA-1:exon:9864;Parent=Msa0202990-mRNA-1
chr2_1	exon	26147473	26147645	26147473	ID=Msa0202990-mRNA-1:exon:9863;Parent=Msa0202990-mRNA-1
chr2_1	exon	26147260	26147334	26147260	ID=Msa0202990-mRNA-1:exon:9862;Parent=Msa0202990-mRNA-1
chr2_1	CDS	26148278	26148461	26148278	ID=Msa0202990-mRNA-1:cds;Parent=Msa0202990-mRNA-1
chr2_1	CDS	26147473	26147645	26147473	ID=Msa0202990-mRNA-1:cds;Parent=Msa0202990-mRNA-1
chr2_1	CDS	26147260	26147334	26147260	ID=Msa0202990-mRNA-1:cds;Parent=Msa0202990-mRNA-1

Gene Sequence

>Msa0202990

ATGAACAAGAACAAGTTTCCTTTTACTCCATCACAATGGCAAGAACTTGAACATCAAGCTCTTATTTACAAGTATATGGCTTCTGGTATATCTATTCCACCTGATCTTCTTTTTACCATCAGAAAAAGCTATTTGGACTCTCCTCTTTCTTCAAGACTTTTTCCTACTCAACAACACCAACACTTTGGATGGAACTATCTGCAAATGGGTTTAGGAAGAAAAATAGATCCAGAGCCAGGTAGATGTAGAAGAACAGATGGAAAGAAATGGAGATGTTCAAAAGAAGCATATCCAGATTCTAAATATTGTGAAAGACACATGCATAGAGGAAAAAACCGTTCAAGAAAGCCTGTGGAATTCTACAAACGGGAAACCCTATCAGCGTCTACCTTACCAAGGTGCAAGGAGAATGAAGTTACTGGGCTGATTTAA

Protein sequence

>Msa0202990

MNKNKFPFTPSQWQELEHQALIYKYMASGISIPPDLLFTIRKSYLDSPLSSRLFPTQQHQHFGWNYLQMGLGRKIDPEPGRCRRTDGKKWRCSKEAYPDSKYCERHMHRGKNRSRKPVEFYKRETLSASTLPRCKENEVTGLI*