AlfalfaGEDB | Msa0203320

M. sativa cultivar zhongmu-4 / Msa0203320

KEGG: K04733
GO: GO:0000003 GO:0001558 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006935 GO:0008150 GO:0008152 GO:0009506 GO:0009605 GO:0009856 GO:0009987 GO:0010183 GO:0010769 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0022603 GO:0022604 GO:0030054 GO:0030427 GO:0032501 GO:0032502 GO:0035838 GO:0036211 GO:0040008 GO:0040011 GO:0042221 GO:0042330 GO:0042802 GO:0042995 GO:0043170 GO:0043412 GO:0043900 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044463 GO:0044464 GO:0044706 GO:0045595 GO:0048638 GO:0048856 GO:0048868 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0050918 GO:0051128 GO:0051239 GO:0051286 GO:0051510 GO:0051704 GO:0055044 GO:0060284 GO:0065007 GO:0071704 GO:0071944 GO:0080092 GO:0090404 GO:0090406 GO:0120025 GO:0120038 GO:0140096 GO:1901564 GO:2000241
PFAM: PF08263.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0203320	AES65591.1	95.577	633	25	2	1	633	1	630	0.0	1237

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0203320	sp\|Q9M1L7\|PRK3_ARATH	49.216	638	302	8	10	633	4	633	0.0	616

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0203320	G7IIP0	95.577	633	25	2	1	633	1	630	0.0	1237

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0203320	MtrunA17_Chr4g0070291	66.563	643	186	9	3	633	2	627	0.0	840
Msa0203320	MtrunA17_Chr2g0301071	94.369	444	22	2	1	444	1	441	0.0	796
Msa0203320	MtrunA17_Chr8g0344471	49.769	649	272	10	30	632	31	671	0.0	634
Msa0203320	MtrunA17_Chr8g0388991	38.547	633	358	8	6	632	10	617	2.83e-148	442
Msa0203320	MtrunA17_Chr8g0379571	39.553	627	352	10	9	632	54	656	8.78e-148	443
Msa0203320	MtrunA17_Chr2g0334251	38.162	642	383	8	3	632	13	652	2.15e-146	440
Msa0203320	MtrunA17_Chr1g0147701	39.185	638	341	12	8	632	12	615	1.23e-144	434
Msa0203320	MtrunA17_Chr2g0301061	96.907	194	6	0	440	633	4	197	1.50e-136	397
Msa0203320	MtrunA17_Chr3g0128551	33.029	657	373	20	1	632	1	615	3.36e-103	327
Msa0203320	MtrunA17_Chr4g0062911	34.472	615	370	14	28	632	27	618	5.51e-100	318

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0203320	AT3G42880.1	49.216	638	302	8	10	633	4	633	0.0	616
Msa0203320	AT5G20690.1	47.962	638	289	10	30	632	29	658	0.0	609
Msa0203320	AT5G35390.1	39.874	637	367	9	1	632	4	629	1.49e-156	466
Msa0203320	AT1G72460.1	42.212	642	332	14	9	628	6	630	2.56e-149	446
Msa0203320	AT3G20190.1	39.043	648	352	11	7	632	21	647	4.58e-147	442
Msa0203320	AT4G31250.1	39.051	653	346	12	7	631	12	640	5.45e-142	429
Msa0203320	AT1G50610.1	39.320	618	322	11	17	607	33	624	3.78e-137	416
Msa0203320	AT2G07040.1	38.424	609	349	9	30	631	23	612	5.23e-137	415
Msa0203320	AT3G02880.1	36.557	610	357	15	30	631	28	615	3.87e-109	342
Msa0203320	AT5G16590.1	35.473	592	346	14	52	631	46	613	1.49e-106	335

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Find 166 sgRNAs with CRISPR-Local

Find 236 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAATGGAGAGAGAGAGG+AGG	0.550539	2_1:+27083897	None:intergenic
AAAGCAGCTGCTGAGGTATT+GGG	0.325609	2_1:-27082695	Msa0203320:CDS
AAAGCATAATCACTTAATAG+AGG	0.525083	2_1:+27081725	None:intergenic
AAAGCTGATGTTTATTGTCT+CGG	0.480020	2_1:-27081614	Msa0203320:CDS
AACAACCCCGACCCACGTAC+CGG	0.516469	2_1:+27083757	None:intergenic
AACCGCTTAAAGATTGCTAA+AGG	0.551469	2_1:-27081851	Msa0203320:CDS
AACTGGGCGAAGAAATCTCC+AGG	0.532898	2_1:+27083530	None:intergenic
AAGAGTTGGGAGACGGAACG+TGG	0.589786	2_1:-27082825	Msa0203320:CDS
AAGCAGCTGCTGAGGTATTG+GGG	0.599251	2_1:-27082694	Msa0203320:CDS
AAGCCATACTTTCTTCAAGG+AGG	0.639778	2_1:+27083505	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGACACGAAAACTTTATAGA+AGG	-	chr2_1:27082245-27082264	Msa0203320:intron	30.0%
GACACGAAAACTTTATAGAA+GGG	-	chr2_1:27082246-27082265	Msa0203320:intron	30.0%
ATGAGGGAGATGAATAAAAT+TGG	-	chr2_1:27082670-27082689	Msa0203320:CDS	30.0%
TGAGGGAGATGAATAAAATT+GGG	-	chr2_1:27082671-27082690	Msa0203320:CDS	30.0%
ACATATAACAAGCTTCCTTT+AGG	+	chr2_1:27082814-27082833	None:intergenic	30.0%
TTTATGTTTCGGCAAAATTG+TGG	+	chr2_1:27083006-27083025	None:intergenic	30.0%
AAACTTAGGTCAAGTTAATC+TGG	+	chr2_1:27083108-27083127	None:intergenic	30.0%
GATACCGAGTTATAAAACTT+AGG	+	chr2_1:27083122-27083141	None:intergenic	30.0%
TTCCTTTAGCAATCTTTAAG+CGG	+	chr2_1:27083429-27083448	None:intergenic	30.0%
AGGTTGAAAATTCAGTGTAA+AGG	+	chr2_1:27083471-27083490	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	27081340	27083957	27081340	ID=Msa0203320;Name=Msa0203320
chr2_1	mRNA	27081340	27083957	27081340	ID=Msa0203320-mRNA-1;Parent=Msa0203320;Name=Msa0203320-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0\|0\|0\|1\|1\|1\|3\|0\|633
chr2_1	exon	27083058	27083957	27083058	ID=Msa0203320-mRNA-1:exon:10040;Parent=Msa0203320-mRNA-1
chr2_1	exon	27082456	27082885	27082456	ID=Msa0203320-mRNA-1:exon:10039;Parent=Msa0203320-mRNA-1
chr2_1	exon	27081340	27081911	27081340	ID=Msa0203320-mRNA-1:exon:10038;Parent=Msa0203320-mRNA-1
chr2_1	CDS	27083058	27083957	27083058	ID=Msa0203320-mRNA-1:cds;Parent=Msa0203320-mRNA-1
chr2_1	CDS	27082456	27082885	27082456	ID=Msa0203320-mRNA-1:cds;Parent=Msa0203320-mRNA-1
chr2_1	CDS	27081340	27081911	27081340	ID=Msa0203320-mRNA-1:cds;Parent=Msa0203320-mRNA-1

Gene Sequence

>Msa0203320

ATGGCCGCTGCTGCCAACAGCATCCTCCTCCTCCTCCTCCTCCTCTCTCTCTCCATTCTTTCTCTCTCCACCGCACAACCACCACCTTCAGAAACACAAGCCCTCCTTAAACTAAAACAATCCTTAACAAACTCAGACAAAATCTTGTCAACATGGATACCAAATGTCTCTCCATGTTCCGGTACGTGGGTCGGGGTTGTTTGCTTCGACAATGTCATCACAGGCCTTCATTTATCAGACCTTCAACTCTCCGGTACAATAGACGTCGATGCCATCGTGGAGATTCGAGGCCTGAGGACGCTTAGCTTTGTCAACAACTCCTTCACCGGTCCTATTCCGCAGTTCAACAAACTCGGAGCCATAAAATCTCTTCTCCTCCAACAAAACCAATTCTCTGGTCCAATTCCTGGAGATTTCTTCGCCCAGTTAACCTCCTTGAAGAAAGTATGGCTTTCAGGTAATAAATTCTCCGGCAACATTCCGCCTTCGTTAACCGAACTTGATCTTCTCAAAGAGCTTCACCTCGAAGGGAATGAATTCTCCGGTCAGCTTCCGAGTCTTAAACAAGATATCAAATCATTCGATGTGTCGAATAACAAGTTAGAAGGTCCGATTCCCGAGAGTCTCGTGAGATTCGGGCCGGTTTCTTTTGCTGGAAATGAAGGACTATGTGGAAAACCGTTGGAAAAACAATGTGACAGTTCATCTGCTGCTGAATATACGCTACCGGATTCTAAAACAGAAAGTTCCAGTTCGAGTTGGGTGCCTCAAGTGATAGGACTTGTGGTTTTGGCAGTTATAGCGGCGGTTATAGGTCTGTTTGTGAAGTCGAGGCAGCGGAGAAGAGAAGATGATTTTAGCGTGGTGAGCCGAGACAGCAGTGTCGACGAAGTGATGCAGGTGCGCGTGCGGATAAGTAGAGCTTCATCTGCATCAGAAAGAGTTGGGAGACGGAACGTGGGAGAATCTTCGAAAAGAGGAGGAACGGGGGGTGGGAGTAGAAATGGGATAGGTGATATTGTGATGGTGAATGATGAGAAGGGTTCATTTGGTTTACAAGATTTGATGAAAGCAGCTGCTGAGGTATTGGGGAACGGTGGATTGGGATCGGCTTATAAGGCCGCGATGGCGAGTGGGTTGAGTGTAGTGGTGAAGAGAATGAGGGAGATGAATAAAATTGGGAAAGATGTGTTTGATGCTGAGATGAGACAGTTTGGGAGGATTAGACATGCTAATATTTTGACTCCTTTGGCTTATCATTATCGTCGAGAGGAGAAGCTTTTTGTTACTGAGTATAAGCCTAAAGGAAGCTTGTTATATGTCTTACACGGTGACAGAGGAATGTCCCATGCTGAGTTAACATGGCCAAACCGCTTAAAGATTGCTAAAGGAATTGCAAGAGGGTTAAGCTTCCTTTACACTGAATTTTCAACCTATGACTTACCACATGGAAATCTCAAATCAAGCAATGTTATACTAACTAATGATTATGAGCCTCTATTAAGTGATTATGCTTTTCAACCACTAATCAACCCAAGCATTGCAGTGCAATCAATGTTTGCATACAAAACACCAGATTACGTTCAAAACCAAAAGCTTTCGCAGAAAGCTGATGTTTATTGTCTCGGAATCATCATTCTTGAACTCATTACAGGAAAATTCCCTTCTCAGTATCATAGTAACGGTAAAGGTGGAACTGATGTTGTTCAATGGGTCCTCACAGCAATTTCTGAGAGAAGAGAAGCTGAATTGATTGATCCTGAGTTAAAAAATAATGCATCAAATTCAACCAGTAATATGCTGCAACTTCTCCTTATTGGTGCTGCTTGTACCGAAAGCAATCCGGAACAAAGACTACACATGAAGGAAGCTATTAGAAGGATAGAGGAAGTACAACTCTAA

Protein sequence

>Msa0203320

MAAAANSILLLLLLLSLSILSLSTAQPPPSETQALLKLKQSLTNSDKILSTWIPNVSPCSGTWVGVVCFDNVITGLHLSDLQLSGTIDVDAIVEIRGLRTLSFVNNSFTGPIPQFNKLGAIKSLLLQQNQFSGPIPGDFFAQLTSLKKVWLSGNKFSGNIPPSLTELDLLKELHLEGNEFSGQLPSLKQDIKSFDVSNNKLEGPIPESLVRFGPVSFAGNEGLCGKPLEKQCDSSSAAEYTLPDSKTESSSSSWVPQVIGLVVLAVIAAVIGLFVKSRQRRREDDFSVVSRDSSVDEVMQVRVRISRASSASERVGRRNVGESSKRGGTGGGSRNGIGDIVMVNDEKGSFGLQDLMKAAAEVLGNGGLGSAYKAAMASGLSVVVKRMREMNKIGKDVFDAEMRQFGRIRHANILTPLAYHYRREEKLFVTEYKPKGSLLYVLHGDRGMSHAELTWPNRLKIAKGIARGLSFLYTEFSTYDLPHGNLKSSNVILTNDYEPLLSDYAFQPLINPSIAVQSMFAYKTPDYVQNQKLSQKADVYCLGIIILELITGKFPSQYHSNGKGGTDVVQWVLTAISERREAELIDPELKNNASNSTSNMLQLLLIGAACTESNPEQRLHMKEAIRRIEEVQL*