AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0207740

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0207740 MtrunA17_Chr2g0306641 97.800 500 10 1 1 500 3 501 0.0 1008
Msa0207740 MtrunA17_Chr8g0376381 50.588 510 198 12 14 486 30 522 2.79e-163 473
Msa0207740 MtrunA17_Chr3g0110971 46.939 539 209 12 14 486 60 587 1.36e-158 463
Msa0207740 MtrunA17_Chr7g0224271 42.105 399 190 9 114 486 290 673 4.77e-92 294
Msa0207740 MtrunA17_Chr4g0076091 33.410 437 268 10 65 485 113 542 1.91e-69 231
Msa0207740 MtrunA17_Chr2g0319091 36.990 392 220 10 113 485 195 578 1.69e-67 227
Msa0207740 MtrunA17_Chr4g0057291 35.024 414 239 9 94 488 262 664 2.64e-66 226
Msa0207740 MtrunA17_Chr8g0357601 35.661 401 214 13 116 490 174 556 3.37e-66 223
Msa0207740 MtrunA17_Chr5g0445331 36.272 397 216 13 113 486 150 532 3.43e-66 222
Msa0207740 MtrunA17_Chr3g0101141 38.287 397 204 14 113 486 164 542 1.88e-65 221
Msa0207740 MtrunA17_Chr5g0447691 36.340 388 216 12 117 486 170 544 1.09e-64 219
Msa0207740 MtrunA17_Chr1g0159461 35.059 425 242 12 86 486 178 592 5.10e-64 218
Msa0207740 MtrunA17_Chr7g0245601 37.366 372 199 11 128 486 442 792 9.99e-63 218
Msa0207740 MtrunA17_Chr3g0125811 32.770 473 274 16 36 486 120 570 3.43e-61 210
Msa0207740 MtrunA17_Chr4g0057891 34.568 405 216 14 114 487 135 521 1.31e-60 207
Msa0207740 MtrunA17_Chr2g0290241 32.000 400 249 12 104 487 34 426 1.51e-57 197
Msa0207740 MtrunA17_Chr4g0038761 35.789 380 208 12 128 491 88 447 1.29e-56 195
Msa0207740 MtrunA17_Chr3g0085681 30.952 420 223 9 125 486 26 436 4.33e-56 193
Msa0207740 MtrunA17_Chr1g0200401 31.294 425 238 16 99 488 56 461 1.19e-54 190
Msa0207740 MtrunA17_Chr2g0328391 32.199 382 239 8 118 485 293 668 2.06e-52 188
Msa0207740 MtrunA17_Chr4g0031721 33.245 379 235 8 119 485 311 683 9.22e-51 184
Msa0207740 MtrunA17_Chr4g0031731 28.856 402 266 6 96 483 228 623 8.16e-50 180
Msa0207740 MtrunA17_Chr4g0038851 29.932 441 232 14 115 487 39 470 1.29e-49 177
Msa0207740 MtrunA17_Chr1g0183641 30.023 443 262 14 64 485 61 476 8.95e-49 174
Msa0207740 MtrunA17_Chr4g0031711 30.412 388 248 7 114 485 351 732 2.18e-48 177
Msa0207740 MtrunA17_Chr3g0114841 33.333 399 230 12 114 485 110 499 2.64e-48 174
Msa0207740 MtrunA17_Chr4g0031701 28.747 407 270 7 91 483 223 623 2.69e-48 176
Msa0207740 MtrunA17_Chr2g0328341 32.199 382 239 8 116 485 264 637 5.40e-48 175
Msa0207740 MtrunA17_Chr5g0396491 29.372 446 229 12 115 487 47 479 1.28e-47 171
Msa0207740 MtrunA17_Chr3g0085361 32.092 349 177 8 190 486 10 350 2.21e-46 165
Msa0207740 MtrunA17_Chr4g0039661 31.974 466 266 18 56 486 106 555 4.53e-46 169
Msa0207740 MtrunA17_Chr7g0238891 30.628 382 243 8 119 486 367 740 1.84e-45 169
Msa0207740 MtrunA17_Chr2g0294461 29.545 396 250 8 114 488 200 587 2.11e-45 167
Msa0207740 MtrunA17_Chr5g0424131 32.518 409 241 14 106 488 102 501 4.49e-45 165
Msa0207740 MtrunA17_Chr2g0328411 30.446 404 261 12 92 485 260 653 4.73e-45 167
Msa0207740 MtrunA17_Chr4g0031741 31.854 383 237 12 117 485 277 649 1.87e-44 166
Msa0207740 MtrunA17_Chr2g0328381 29.449 472 294 14 31 485 191 640 2.31e-43 162
Msa0207740 MtrunA17_Chr2g0328431 29.310 406 263 11 92 485 260 653 3.47e-43 162
Msa0207740 MtrunA17_Chr2g0328371 37.154 253 148 6 194 441 5 251 2.23e-42 151
Msa0207740 MtrunA17_Chr7g0230771 32.105 380 224 9 121 486 372 731 5.01e-41 157
Msa0207740 MtrunA17_Chr8g0383981 31.088 386 219 15 121 485 136 495 1.90e-40 152
Msa0207740 MtrunA17_Chr2g0294471 27.157 394 259 9 114 488 194 578 2.31e-40 153
Msa0207740 MtrunA17_Chr7g0230731 31.579 380 226 9 121 486 369 728 3.27e-39 151
Msa0207740 MtrunA17_Chr5g0401131 29.086 361 235 6 147 486 130 490 4.88e-38 145
Msa0207740 MtrunA17_Chr2g0328401 29.477 363 227 12 107 453 273 622 3.30e-37 145
Msa0207740 MtrunA17_Chr2g0328361 29.288 379 229 10 116 485 266 614 3.69e-36 141
Msa0207740 MtrunA17_Chr7g0270031 27.877 391 249 11 114 486 179 554 9.51e-35 137
Msa0207740 MtrunA17_Chr6g0468561 27.720 386 240 9 123 486 294 662 1.34e-34 137
Msa0207740 MtrunA17_Chr1g0194621 27.136 398 250 11 114 486 136 518 2.45e-33 132
Msa0207740 MtrunA17_Chr4g0012071 27.068 399 256 13 105 487 173 552 9.62e-33 131
Msa0207740 MtrunA17_Chr7g0236851 32.646 291 179 9 204 485 374 656 2.45e-32 130
Msa0207740 MtrunA17_Chr5g0401421 26.730 419 244 13 123 485 34 445 1.88e-31 126
Msa0207740 MtrunA17_Chr4g0053901 25.647 425 239 14 123 485 58 467 5.58e-29 119
Msa0207740 MtrunA17_Chr2g0329591 25.852 352 224 8 162 486 110 451 9.91e-28 115
Msa0207740 MtrunA17_Chr8g0373561 26.842 380 247 9 121 486 179 541 1.18e-26 113
Msa0207740 MtrunA17_Chr8g0344101 27.157 313 192 9 197 486 301 600 4.51e-22 99.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0207740 AT1G14920.1 48.350 515 212 11 14 486 27 529 5.93e-161 467
Msa0207740 AT3G03450.1 47.554 511 216 13 17 486 46 545 1.57e-150 441
Msa0207740 AT1G66350.1 49.595 494 210 15 14 486 31 506 8.21e-149 436
Msa0207740 AT5G17490.1 46.169 496 234 11 13 486 32 516 1.60e-143 422
Msa0207740 AT2G01570.1 54.922 386 162 6 105 486 204 581 1.46e-142 422
Msa0207740 AT2G01570.1 53.846 65 29 1 15 78 44 108 4.04e-13 72.0
Msa0207740 AT5G48150.1 36.456 395 219 9 111 486 109 490 2.07e-66 222
Msa0207740 AT5G48150.4 36.456 395 219 9 111 486 109 490 2.07e-66 222
Msa0207740 AT5G48150.2 36.456 395 219 9 111 486 109 490 2.07e-66 222
Msa0207740 AT5G48150.3 36.456 395 219 9 111 486 109 490 2.07e-66 222
Msa0207740 AT2G04890.1 36.364 385 221 8 113 486 42 413 3.20e-66 219
Msa0207740 AT1G50600.3 35.750 400 236 8 100 486 135 526 5.00e-66 222
Msa0207740 AT1G50600.2 35.750 400 236 8 100 486 210 601 1.28e-65 223
Msa0207740 AT1G50600.1 35.750 400 236 8 100 486 206 597 1.54e-65 223
Msa0207740 AT5G41920.1 35.696 395 219 13 112 490 29 404 1.04e-62 210
Msa0207740 AT1G21450.1 34.500 400 246 8 98 486 199 593 6.98e-61 210
Msa0207740 AT3G54220.1 35.310 371 209 9 128 485 297 649 2.16e-59 207
Msa0207740 AT1G50420.1 31.614 446 217 11 115 486 48 479 2.61e-58 201
Msa0207740 AT1G63100.1 34.606 393 220 10 119 485 247 628 3.15e-58 204
Msa0207740 AT1G63100.2 34.606 393 220 10 119 485 272 653 5.68e-58 203
Msa0207740 AT4G17230.1 35.219 389 221 12 119 489 153 528 2.98e-55 193
Msa0207740 AT4G17230.2 35.219 389 221 12 119 489 153 528 2.98e-55 193
Msa0207740 AT1G55580.1 34.359 390 217 13 116 486 76 445 2.79e-49 176
Msa0207740 AT1G07530.1 32.570 393 245 9 106 485 379 764 4.61e-49 180
Msa0207740 AT5G66770.1 33.148 359 215 10 145 486 234 584 1.24e-47 174
Msa0207740 AT5G59450.1 29.310 406 262 9 98 486 201 598 1.53e-47 174
Msa0207740 AT3G46600.3 29.703 404 265 9 95 486 151 547 1.18e-46 171
Msa0207740 AT3G46600.1 29.703 404 265 9 95 486 183 579 1.55e-46 171
Msa0207740 AT3G46600.2 30.423 378 244 9 121 486 1 371 9.89e-46 164
Msa0207740 AT2G29060.2 30.729 384 245 10 121 490 281 657 1.00e-43 164
Msa0207740 AT3G50650.1 33.721 344 201 8 160 486 209 542 1.20e-43 162
Msa0207740 AT2G29060.1 30.729 384 245 10 121 490 318 694 1.51e-43 164
Msa0207740 AT2G29065.1 27.436 390 241 9 124 485 252 627 1.81e-38 149
Msa0207740 AT1G07520.2 27.750 400 246 12 114 485 144 528 2.17e-38 147
Msa0207740 AT1G07520.4 27.750 400 246 12 114 485 144 528 2.17e-38 147
Msa0207740 AT1G07520.1 27.750 400 246 12 114 485 308 692 3.40e-38 149
Msa0207740 AT1G07520.3 27.750 400 246 12 114 485 309 693 3.65e-38 148
Msa0207740 AT4G08250.1 29.668 391 247 17 113 485 96 476 3.76e-38 145
Msa0207740 AT2G37650.2 28.052 385 247 10 119 486 330 701 5.34e-36 142
Msa0207740 AT2G37650.1 28.052 385 247 10 119 486 342 713 5.56e-36 142
Msa0207740 AT5G52510.1 29.565 345 215 9 162 486 304 640 1.13e-35 140
Msa0207740 AT4G00150.1 29.310 348 220 9 148 486 224 554 2.86e-34 136
Msa0207740 AT4G37650.1 27.882 373 229 11 148 486 163 529 8.48e-33 131
Msa0207740 AT2G45160.1 29.558 362 216 11 148 486 294 639 1.09e-31 129
Msa0207740 AT3G49950.1 27.551 392 250 14 123 490 29 410 5.53e-29 119
Msa0207740 AT3G60630.1 28.758 306 192 9 194 486 330 622 6.17e-29 120
Msa0207740 AT4G36710.1 26.555 418 272 12 84 486 88 485 2.08e-28 118
Msa0207740 AT3G13840.1 25.309 324 198 11 181 486 213 510 1.00e-16 83.2

Find 136 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATAACGAGAACGGGTTTA+TGG 0.172499 2_1:-40617071 Msa0207740:CDS
TTCGGGTATTGAAATGGTTT+TGG 0.204240 2_1:-40617149 Msa0207740:CDS
CGGGTTCAGACCGTTGCATT+TGG 0.261310 2_1:-40616855 Msa0207740:CDS
CCATAAACCCGTTCTCGTTA+TGG 0.276597 2_1:+40617071 None:intergenic
CCGGCCTTTGATTGCGGCTT+CGG 0.318572 2_1:-40616729 Msa0207740:CDS
TCAATACCCGAATGAGAATT+CGG 0.329389 2_1:+40617160 None:intergenic
TTTCAGCTGCATCGTCTTCT+CGG 0.338452 2_1:-40617196 Msa0207740:CDS
CGGCATTGGTCCGCCGTCGC+TGG 0.354188 2_1:-40617383 Msa0207740:CDS
CCTCCGTTTCTCCGGCTTAC+CGG 0.355777 2_1:-40617403 Msa0207740:CDS
CTTTGATTGCGGCTTCGGCT+TGG 0.374762 2_1:-40616724 Msa0207740:CDS
CACTATGATCCCTCCGACAT+TGG 0.380904 2_1:-40617979 Msa0207740:CDS
CGTGACAACCTCCGTGAAAT+CGG 0.385600 2_1:-40617355 Msa0207740:CDS
ATCCCTCCGACATTGGCAAT+TGG 0.385878 2_1:-40617972 Msa0207740:CDS
GAATCAAGGGAGCTTGACTT+TGG 0.395394 2_1:-40616762 Msa0207740:CDS
TTCTCATTCGGGTATTGAAA+TGG 0.397931 2_1:-40617155 Msa0207740:CDS
GGGTTCAGACCGTTGCATTT+GGG 0.405803 2_1:-40616854 Msa0207740:CDS
TTTCTCCGGCTTACCGGCAT+TGG 0.406008 2_1:-40617397 Msa0207740:CDS
TGACTCGCTTGAAGCGTGTC+CGG 0.418691 2_1:-40617008 Msa0207740:CDS
GCTTTGAACCGTCGCATCTT+CGG 0.420783 2_1:-40617649 Msa0207740:CDS
TTCGAGTATAGCTTGATTTG+CGG 0.426009 2_1:+40617528 None:intergenic
AACGTTAGATTCGCCTTTCG+CGG 0.429000 2_1:-40617301 Msa0207740:CDS
CCACGCCGCAACGCCGCGAA+AGG 0.429090 2_1:+40617288 None:intergenic
TGTAAAGCCATGGATGCTTC+AGG 0.430657 2_1:-40617254 Msa0207740:CDS
AATCAAGGGAGCTTGACTTT+GGG 0.434762 2_1:-40616761 Msa0207740:CDS
AGAGGCCGTGTGGTCAAATT+CGG 0.437374 2_1:+40617933 None:intergenic
TGACTTTGGGTTGGCATAGC+CGG 0.439605 2_1:-40616748 Msa0207740:CDS
CGGGTTTATGGAACGGTTCA+CGG 0.439903 2_1:-40617059 Msa0207740:CDS
CAAGCTCTTGCTCTTCGTCC+TGG 0.444577 2_1:-40617430 Msa0207740:CDS
CGTAGTGTGCTGCGAGGGAC+CGG 0.445861 2_1:-40616933 Msa0207740:CDS
TCCCTCCGACATTGGCAATT+GGG 0.446214 2_1:-40617971 Msa0207740:CDS
CGGTGAAGTGTGCGAATTTG+AGG 0.451224 2_1:+40617548 None:intergenic
CGAGACGGAGGCCGATTTCA+CGG 0.453634 2_1:+40617344 None:intergenic
ATCCGACCCGAATTCTCATT+CGG 0.456753 2_1:-40617167 Msa0207740:CDS
GGCGGCTGTGCTGAGAGATC+TGG 0.456772 2_1:+40617886 None:intergenic
CAATACCCGAATGAGAATTC+GGG 0.457985 2_1:+40617161 None:intergenic
ATCTCGGCCATAGCTTTATC+CGG 0.466984 2_1:+40616980 None:intergenic
TCCGACCCGAATTCTCATTC+GGG 0.467756 2_1:-40617166 Msa0207740:CDS
ATAGCTTTATCCGGTTCAAC+CGG 0.472673 2_1:+40616989 None:intergenic
AAGCGAACCATAACGAGAAC+GGG 0.473338 2_1:-40617078 Msa0207740:CDS
TGAAGCGTGTCCGGTTGAAC+CGG 0.475946 2_1:-40616999 Msa0207740:CDS
CAGCGACGGCGGACCAATGC+CGG 0.478193 2_1:+40617384 None:intergenic
GACATGAACCGTTGGTTAAG+TGG 0.479723 2_1:-40616898 Msa0207740:CDS
CGACATTTCTCAATTCGCTT+CGG 0.481188 2_1:-40618010 Msa0207740:CDS
AAGGAGCGGCTCGGTAAAGC+CGG 0.483410 2_1:-40616875 Msa0207740:CDS
CCTTTCGCGGCGTTGCGGCG+TGG 0.496391 2_1:-40617288 Msa0207740:CDS
CGGAGAAACGGAGGTCCGCC+AGG 0.496483 2_1:+40617412 None:intergenic
CGTGGTAATCTTCCATTGGC+TGG 0.502641 2_1:-40617757 Msa0207740:CDS
TATTAACTGTCGAAGAAGAT+TGG 0.503596 2_1:+40617845 None:intergenic
TTCGCGGCGTTGCGGCGTGG+CGG 0.506872 2_1:-40617285 Msa0207740:CDS
CTCTCTTTGTAAATACATCT+CGG 0.511803 2_1:+40616964 None:intergenic
CAAAGCGTCGATGAAGTATC+CGG 0.512312 2_1:+40617666 None:intergenic
TGGTGATGGTGGTGGTGTTG+TGG 0.512589 2_1:+40617865 None:intergenic
CTGGCGGACCTCCGTTTCTC+CGG 0.512726 2_1:-40617411 Msa0207740:CDS
GCGGACCAATGCCGGTAAGC+CGG 0.513787 2_1:+40617392 None:intergenic
ACCTCATGCATGGTCTTCAA+TGG 0.514040 2_1:-40617465 Msa0207740:CDS
GAACTCACCTGAAGCATCCA+TGG 0.518449 2_1:+40617247 None:intergenic
GCATAGCCGGCCTTTGATTG+CGG 0.520541 2_1:-40616735 Msa0207740:CDS
ATTCGCCTTTCGCGGCGTTG+CGG 0.526404 2_1:-40617293 Msa0207740:CDS
CAAGCCGAAGCCGCAATCAA+AGG 0.528493 2_1:+40616725 None:intergenic
AAGGGAGCTTGACTTTGGGT+TGG 0.530891 2_1:-40616757 Msa0207740:CDS
TCGTTAATCGAAGGCATGCA+AGG 0.531649 2_1:-40617733 Msa0207740:CDS
AAGAAGATTGGTGATGGTGG+TGG 0.534719 2_1:+40617857 None:intergenic
CCGAAGCCGCAATCAAAGGC+CGG 0.539000 2_1:+40616729 None:intergenic
CGCTCCTTCCACTTAACCAA+CGG 0.539436 2_1:+40616890 None:intergenic
ATGCCGGTAAGCCGGAGAAA+CGG 0.543739 2_1:+40617400 None:intergenic
CGTTCTCGTAAAGAGAAACT+TGG 0.543788 2_1:+40617608 None:intergenic
TCGATTAACGAGCCAGCCAA+TGG 0.545335 2_1:+40617745 None:intergenic
CAAATGCAACGGTCTGAACC+CGG 0.552323 2_1:+40616856 None:intergenic
GCAACGTGGTAATCTTCCAT+TGG 0.552649 2_1:-40617761 Msa0207740:CDS
AGAGAAATCGTAGGGTAAAG+AGG 0.554274 2_1:+40617915 None:intergenic
ATGAACACAATCTCCGGCAT+CGG 0.555034 2_1:-40617697 Msa0207740:CDS
TCTGACTTTGTAGCCGACAT+TGG 0.557024 2_1:+40618101 None:intergenic
GCAAACATGAACACAATCTC+CGG 0.557304 2_1:-40617703 Msa0207740:CDS
TGGAAGATTACCACGTTGCA+CGG 0.558728 2_1:+40617765 None:intergenic
CACCCAATTGCCAATGTCGG+AGG 0.559532 2_1:+40617969 None:intergenic
ATTGATTTCAACCTCATGCA+TGG 0.563574 2_1:-40617475 Msa0207740:CDS
AAAGGCGAATCTAACGTTAA+CGG 0.563777 2_1:+40617306 None:intergenic
AGGAGCGGCTCGGTAAAGCC+GGG 0.565092 2_1:-40616874 Msa0207740:CDS
TCATGTCTCTCAACTCGAGC+CGG 0.568274 2_1:+40616914 None:intergenic
TATTGCGTGGAAGAGAATCA+AGG 0.570726 2_1:-40616776 Msa0207740:CDS
GACAAGCTTCGTAGTAGTGA+TGG 0.571422 2_1:+40617575 None:intergenic
TCCGGCATCGGAAAAGTCGC+CGG 0.573578 2_1:-40617685 Msa0207740:CDS
GAAGCGAACCATAACGAGAA+CGG 0.581490 2_1:-40617079 Msa0207740:CDS
GTCCACCCAATTGCCAATGT+CGG 0.582283 2_1:+40617966 None:intergenic
ACGTTGCACGGAATCTGCAC+AGG 0.591505 2_1:+40617777 None:intergenic
TGATGGTGGTGGTGTTGTGG+CGG 0.592809 2_1:+40617868 None:intergenic
CTTTGTAGCCGACATTGGCA+AGG 0.593493 2_1:+40618106 None:intergenic
AAGTGTGCGAATTTGAGGTA+TGG 0.594726 2_1:+40617553 None:intergenic
GGTTGAACCGGATAAAGCTA+TGG 0.595176 2_1:-40616987 Msa0207740:CDS
TCTCGTAAAGAGAAACTTGG+TGG 0.598426 2_1:+40617611 None:intergenic
CGATTTCACGGAGGTTGTCA+CGG 0.600453 2_1:+40617356 None:intergenic
GGAAGTTCAGAGAAATCGTA+GGG 0.603775 2_1:+40617907 None:intergenic
GCTCTTGCTCTTCGTCCTGG+CGG 0.606990 2_1:-40617427 Msa0207740:CDS
CTCCGACATTGGCAATTGGG+TGG 0.609033 2_1:-40617968 Msa0207740:CDS
ACCCAATTGCCAATGTCGGA+GGG 0.609324 2_1:+40617970 None:intergenic
TGGAAGTTCAGAGAAATCGT+AGG 0.615760 2_1:+40617906 None:intergenic
TGCAACGTAGTGTGCTGCGA+GGG 0.616117 2_1:-40616938 Msa0207740:CDS
AGCGTGCAAGTTCAGCGAGA+CGG 0.617149 2_1:+40617329 None:intergenic
GACGGCCTCCTTGCCAATGT+CGG 0.620771 2_1:-40618114 Msa0207740:CDS
CGTTAATCGAAGGCATGCAA+GGG 0.622830 2_1:-40617732 Msa0207740:CDS
TGAACCGTTGGTTAAGTGGA+AGG 0.623165 2_1:-40616894 Msa0207740:CDS
ACGAGAACGGGTTTATGGAA+CGG 0.624479 2_1:-40617066 Msa0207740:CDS
GCTATACTCGAAGCTTTCAA+CGG 0.625134 2_1:-40617517 Msa0207740:CDS
CAATGACGTGGACACAATCG+TGG 0.629821 2_1:+40617494 None:intergenic
GTTAAGTGGAAGGAGCGGCT+CGG 0.631755 2_1:-40616884 Msa0207740:CDS
TTGGCTGGCTCGTTAATCGA+AGG 0.631890 2_1:-40617742 Msa0207740:CDS
TTCAGCGGAGGGTTATTGCG+TGG 0.637968 2_1:-40616789 Msa0207740:CDS
ATTGCGTGGAAGAGAATCAA+GGG 0.640198 2_1:-40616775 Msa0207740:CDS
AGTTGAGAGACATGAACCGT+TGG 0.643232 2_1:-40616906 Msa0207740:CDS
GCATTCGAACCCAAATGCAA+CGG 0.649133 2_1:+40616845 None:intergenic
GCAAGAGCTTGAATTAACGC+TGG 0.649987 2_1:+40617442 None:intergenic
TCACGGTCATCCAGCGACGG+CGG 0.651033 2_1:+40617373 None:intergenic
CAATGTCGGAGGGATCATAG+TGG 0.653195 2_1:+40617980 None:intergenic
GGTGAGTTCTAAAGAAGCTG+TGG 0.654073 2_1:-40617233 Msa0207740:CDS
GTGCAAGTTCAGCGAGACGG+AGG 0.656208 2_1:+40617332 None:intergenic
GAGTTCTAAAGAAGCTGTGG+CGG 0.658727 2_1:-40617230 Msa0207740:CDS
TTGTCACGGTCATCCAGCGA+CGG 0.659920 2_1:+40617370 None:intergenic
CGTTGGTTAAGTGGAAGGAG+CGG 0.660011 2_1:-40616889 Msa0207740:CDS
TGTAGCCGACATTGGCAAGG+AGG 0.660431 2_1:+40618109 None:intergenic
TCAAAGACAAAAGACATCGA+CGG 0.665997 2_1:-40618132 Msa0207740:CDS
CTGTCGAAGAAGATTGGTGA+TGG 0.666887 2_1:+40617851 None:intergenic
CTGCACAGGTCATCAGCATG+TGG 0.667697 2_1:+40617791 None:intergenic
CCGGTAAGCCGGAGAAACGG+AGG 0.669681 2_1:+40617403 None:intergenic
CCTCTCCGAATTTGACCACA+CGG 0.676826 2_1:-40617938 Msa0207740:CDS
GGCTTGAAGATGTAAAGCCA+TGG 0.681200 2_1:-40617264 Msa0207740:CDS
TCGAAGAAGATTGGTGATGG+TGG 0.682954 2_1:+40617854 None:intergenic
ATGCAACGTAGTGTGCTGCG+AGG 0.694303 2_1:-40616939 Msa0207740:CDS
AGTGTTTCAGCATCTAACAA+AGG 0.702442 2_1:+40616692 None:intergenic
CGTAGGGTAAAGAGGCCGTG+TGG 0.707233 2_1:+40617923 None:intergenic
GCCATTGAAGACCATGCATG+AGG 0.712810 2_1:+40617464 None:intergenic
ACCCGAATGAGAATTCGGGT+CGG 0.713077 2_1:+40617165 None:intergenic
CCGTGTGGTCAAATTCGGAG+AGG 0.714620 2_1:+40617938 None:intergenic
TGAGGTTGAAATCAATGACG+TGG 0.716676 2_1:+40617482 None:intergenic
GACGGAGGCCGATTTCACGG+AGG 0.751201 2_1:+40617347 None:intergenic
TGTGCAGATTCCGTGCAACG+TGG 0.753527 2_1:-40617775 Msa0207740:CDS
GTTTATGGAACGGTTCACGG+AGG 0.758651 2_1:-40617056 Msa0207740:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTAATTTGCATTATTAT+AGG + chr2_1:40616811-40616830 None:intergenic 10.0%
!!! AATTTTCAACTTTTTTTAAA+AGG + chr2_1:40618080-40618099 None:intergenic 10.0%
!!! TTATATATTTTTACAGTTTG+TGG - chr2_1:40617823-40617842 Msa0207740:CDS 15.0%
!! TTTCTTTAATAAGAGTAAGT+AGG - chr2_1:40616834-40616853 Msa0207740:CDS 20.0%
!!! ATTTTCTACAACTTAATTGT+AGG + chr2_1:40616942-40616961 None:intergenic 20.0%
!!! TTTTAATATGACTAGTACTT+TGG - chr2_1:40617113-40617132 Msa0207740:CDS 20.0%
!!! TCAAAAAAGGTTTTCAAATT+TGG - chr2_1:40617168-40617187 Msa0207740:CDS 20.0%
!!! CAAAAAAGGTTTTCAAATTT+GGG - chr2_1:40617169-40617188 Msa0207740:CDS 20.0%
!! TAAATAGATAATGTAGAAAC+TGG - chr2_1:40617332-40617351 Msa0207740:CDS 20.0%
!! TTTATACAATATTGGGATTT+GGG + chr2_1:40617977-40617996 None:intergenic 20.0%
!!! TTTATAATTGAGTTTTAGCT+GGG + chr2_1:40618329-40618348 None:intergenic 20.0%
!! TTTATGTTTCTATTGCAACA+TGG + chr2_1:40616906-40616925 None:intergenic 25.0%
! GTTCCTATAATCATTTGTAA+AGG + chr2_1:40616983-40617002 None:intergenic 25.0%
! ACAAATGATTATAGGAACAT+AGG - chr2_1:40616985-40617004 Msa0207740:CDS 25.0%
!!! TTTCAAGTTAAGTGGTAAAT+TGG + chr2_1:40617212-40617231 None:intergenic 25.0%
!!! AATTAGGATTTCAAGTTAAG+TGG + chr2_1:40617220-40617239 None:intergenic 25.0%
! CTTGAAATCCTAATTATTTG+AGG - chr2_1:40617225-40617244 Msa0207740:CDS 25.0%
! ATCATTTGCCTCAAATAATT+AGG + chr2_1:40617236-40617255 None:intergenic 25.0%
! TACACATTCCTGTTTATATT+TGG - chr2_1:40617751-40617770 Msa0207740:CDS 25.0%
! AACAAATGCCAAATATAAAC+AGG + chr2_1:40617762-40617781 None:intergenic 25.0%
! GTTTATATTTGGCATTTGTT+TGG - chr2_1:40617762-40617781 Msa0207740:CDS 25.0%
! AAAATCGTAACAAATCACAA+AGG - chr2_1:40617883-40617902 Msa0207740:CDS 25.0%
! TAACAAATCACAAAGGTTAA+TGG - chr2_1:40617890-40617909 Msa0207740:CDS 25.0%
! GTTTATACAATATTGGGATT+TGG + chr2_1:40617978-40617997 None:intergenic 25.0%
!! GATGCTGTTTATACAATATT+GGG + chr2_1:40617984-40618003 None:intergenic 25.0%
!! AGATGCTGTTTATACAATAT+TGG + chr2_1:40617985-40618004 None:intergenic 25.0%
! AAATTATCAAAAGCCAACTT+TGG - chr2_1:40618005-40618024 Msa0207740:CDS 25.0%
! AAATCAAGTAGAGAAAAAAG+AGG + chr2_1:40618248-40618267 None:intergenic 25.0%
! ATGTAATGTAATGTATAGAG+TGG + chr2_1:40618292-40618311 None:intergenic 25.0%
!!! GTTTATAATTGAGTTTTAGC+TGG + chr2_1:40618330-40618349 None:intergenic 25.0%
!!! TGGTAAGTTTTGAATTCTTA+TGG - chr2_1:40618422-40618441 Msa0207740:intron 25.0%
AGGTACTTAATTAAGCAAGT+AGG - chr2_1:40616854-40616873 Msa0207740:CDS 30.0%
GGTACTTAATTAAGCAAGTA+GGG - chr2_1:40616855-40616874 Msa0207740:CDS 30.0%
TTAGCAGAGACAAAATCAAA+GGG - chr2_1:40616880-40616899 Msa0207740:CDS 30.0%
GTTGCAATAGAAACATAAAC+AGG - chr2_1:40616907-40616926 Msa0207740:CDS 30.0%
CAGCCTTTACAAATGATTAT+AGG - chr2_1:40616977-40616996 Msa0207740:CDS 30.0%
! TGTAAAGTCACTTTGTGTAT+CGG + chr2_1:40617066-40617085 None:intergenic 30.0%
GAGTTCATTACACTCAAAAA+AGG - chr2_1:40617155-40617174 Msa0207740:CDS 30.0%
! TAGAGAATGCTAAACTGTTT+TGG + chr2_1:40617284-40617303 None:intergenic 30.0%
AATACAAATTATCACCTGCT+AGG - chr2_1:40617604-40617623 Msa0207740:CDS 30.0%
AAATAACAGTATACCCAAGT+TGG - chr2_1:40617693-40617712 Msa0207740:CDS 30.0%
! TTTTACTCCAATTCCAACTT+GGG + chr2_1:40617709-40617728 None:intergenic 30.0%
! ATTTTACTCCAATTCCAACT+TGG + chr2_1:40617710-40617729 None:intergenic 30.0%
AGTTGGAATTGGAGTAAAAT+TGG - chr2_1:40617710-40617729 Msa0207740:CDS 30.0%
TACAGTTGTACTATAGTTTC+TGG - chr2_1:40617854-40617873 Msa0207740:CDS 30.0%
!!! GTGATTTGTTACGATTTTCA+GGG + chr2_1:40617881-40617900 None:intergenic 30.0%
!!! TGTGATTTGTTACGATTTTC+AGG + chr2_1:40617882-40617901 None:intergenic 30.0%
!!! CTTCAATTTTTAGTAGCCTT+AGG + chr2_1:40618033-40618052 None:intergenic 30.0%
CTAAGGCTACTAAAAATTGA+AGG - chr2_1:40618031-40618050 Msa0207740:CDS 30.0%
GTAAAATGTATGAGCATGAT+GGG + chr2_1:40618173-40618192 None:intergenic 30.0%
TGTAAAATGTATGAGCATGA+TGG + chr2_1:40618174-40618193 None:intergenic 30.0%
GTGATGTGTGAGAAAATTAA+GGG + chr2_1:40618208-40618227 None:intergenic 30.0%
AGTGATGTGTGAGAAAATTA+AGG + chr2_1:40618209-40618228 None:intergenic 30.0%
AATGTAATGTATAGAGTGGT+GGG + chr2_1:40618288-40618307 None:intergenic 30.0%
TAATGTAATGTATAGAGTGG+TGG + chr2_1:40618289-40618308 None:intergenic 30.0%
AACTTATAAGTGTTAGTGTG+TGG + chr2_1:40618392-40618411 None:intergenic 30.0%
CTTATAAGTTCAAAACTGTG+TGG - chr2_1:40618402-40618421 Msa0207740:intron 30.0%
TATTAACTGTCGAAGAAGAT+TGG + chr2_1:40618761-40618780 None:intergenic 30.0%
! TCAACAACTGTAACGATTTT+CGG + chr2_1:40619500-40619519 None:intergenic 30.0%
CTCTCTTTGTAAATACATCT+CGG + chr2_1:40619642-40619661 None:intergenic 30.0%
TACAATGACGTTCTACTTGA+AGG - chr2_1:40616765-40616784 Msa0207740:CDS 35.0%
! GAAGGAAAGACTGTACTTTT+AGG - chr2_1:40616783-40616802 Msa0207740:CDS 35.0%
CTTAGCAGAGACAAAATCAA+AGG - chr2_1:40616879-40616898 Msa0207740:CDS 35.0%
CGGTCTTAGAAGAAATTGTT+TGG + chr2_1:40617046-40617065 None:intergenic 35.0%
AGCTAAATTTCTCCATGTGT+AGG - chr2_1:40617461-40617480 Msa0207740:CDS 35.0%
AGGTTTAGCAGGTATAAGAT+AGG - chr2_1:40617624-40617643 Msa0207740:CDS 35.0%
CAAATCACAAAGGTTAATGG+TGG - chr2_1:40617893-40617912 Msa0207740:CDS 35.0%
! GATGTCTTTTGTCTTTGATG+GGG + chr2_1:40618469-40618488 None:intergenic 35.0%
! CGATGTCTTTTGTCTTTGAT+GGG + chr2_1:40618470-40618489 None:intergenic 35.0%
! TCGATGTCTTTTGTCTTTGA+TGG + chr2_1:40618471-40618490 None:intergenic 35.0%
TCAAAGACAAAAGACATCGA+CGG - chr2_1:40618471-40618490 Msa0207740:intron 35.0%
! TTCGAGTATAGCTTGATTTG+CGG + chr2_1:40619078-40619097 None:intergenic 35.0%
! ATTGATTTCAACCTCATGCA+TGG - chr2_1:40619128-40619147 Msa0207740:intron 35.0%
AAAGGCGAATCTAACGTTAA+CGG + chr2_1:40619300-40619319 None:intergenic 35.0%
TCAATACCCGAATGAGAATT+CGG + chr2_1:40619446-40619465 None:intergenic 35.0%
!! TTCTCATTCGGGTATTGAAA+TGG - chr2_1:40619448-40619467 Msa0207740:intron 35.0%
!!! TTCGGGTATTGAAATGGTTT+TGG - chr2_1:40619454-40619473 Msa0207740:intron 35.0%
!!! TCGGGTATTGAAATGGTTTT+GGG - chr2_1:40619455-40619474 Msa0207740:intron 35.0%
!!! GTATTGAAATGGTTTTGGGT+TGG - chr2_1:40619459-40619478 Msa0207740:intron 35.0%
AGTGTTTCAGCATCTAACAA+AGG + chr2_1:40619914-40619933 None:intergenic 35.0%
!! GTGTCACAGAAAGCATCTTA+AGG + chr2_1:40616720-40616739 None:intergenic 40.0%
AAGATGCTTTCTGTGACACA+TGG - chr2_1:40616721-40616740 Msa0207740:CDS 40.0%
! AGGCAAATGATAGTGACAGT+GGG - chr2_1:40617245-40617264 Msa0207740:CDS 40.0%
TGTACTTCATAGCCTACACA+TGG + chr2_1:40617476-40617495 None:intergenic 40.0%
ATGTGTAGGCTATGAAGTAC+AGG - chr2_1:40617475-40617494 Msa0207740:CDS 40.0%
AGGTTATGTGTAGTATACCG+TGG - chr2_1:40617495-40617514 Msa0207740:CDS 40.0%
GGTTATGTGTAGTATACCGT+GGG - chr2_1:40617496-40617515 Msa0207740:CDS 40.0%
CTTTCTTCATTTAGCACCCA+CGG + chr2_1:40617515-40617534 None:intergenic 40.0%
AAAACAAGCTCGTGAAAGCA+AGG - chr2_1:40617540-40617559 Msa0207740:CDS 40.0%
TTATACCTGCTAAACCTAGC+AGG + chr2_1:40617621-40617640 None:intergenic 40.0%
! CAGTATACCCAAGTTGGAAT+TGG - chr2_1:40617699-40617718 Msa0207740:CDS 40.0%
! AAAGGTTAATGGTGGTAGTG+TGG - chr2_1:40617901-40617920 Msa0207740:CDS 40.0%
AAAGCCAACTTTGGTACCTA+AGG - chr2_1:40618014-40618033 Msa0207740:CDS 40.0%
CGACATTTCTCAATTCGCTT+CGG - chr2_1:40618593-40618612 Msa0207740:intron 40.0%
AGAGAAATCGTAGGGTAAAG+AGG + chr2_1:40618691-40618710 None:intergenic 40.0%
GGAAGTTCAGAGAAATCGTA+GGG + chr2_1:40618699-40618718 None:intergenic 40.0%
TGGAAGTTCAGAGAAATCGT+AGG + chr2_1:40618700-40618719 None:intergenic 40.0%
GCAAACATGAACACAATCTC+CGG - chr2_1:40618900-40618919 Msa0207740:intron 40.0%
!!! TATTTTGACCGAAGATGCGA+CGG + chr2_1:40618965-40618984 None:intergenic 40.0%
TCTCGTAAAGAGAAACTTGG+TGG + chr2_1:40618995-40619014 None:intergenic 40.0%
CGTTCTCGTAAAGAGAAACT+TGG + chr2_1:40618998-40619017 None:intergenic 40.0%
! AAGTGTGCGAATTTGAGGTA+TGG + chr2_1:40619053-40619072 None:intergenic 40.0%
GCTATACTCGAAGCTTTCAA+CGG - chr2_1:40619086-40619105 Msa0207740:intron 40.0%
TGAGGTTGAAATCAATGACG+TGG + chr2_1:40619124-40619143 None:intergenic 40.0%
CAATACCCGAATGAGAATTC+GGG + chr2_1:40619445-40619464 None:intergenic 40.0%
!!! AAATGGTTTTGGGTTGGATC+CGG - chr2_1:40619465-40619484 Msa0207740:intron 40.0%
! CGATTTTCGGATTCAAACTC+CGG + chr2_1:40619487-40619506 None:intergenic 40.0%
!! ATAGCTTTATCCGGTTCAAC+CGG + chr2_1:40619617-40619636 None:intergenic 40.0%
!!! GTTGTTGACTCTGTTTTCAG+CGG - chr2_1:40619799-40619818 Msa0207740:intron 40.0%
TATTGCGTGGAAGAGAATCA+AGG - chr2_1:40619827-40619846 Msa0207740:intron 40.0%
ATTGCGTGGAAGAGAATCAA+GGG - chr2_1:40619828-40619847 Msa0207740:intron 40.0%
AATCAAGGGAGCTTGACTTT+GGG - chr2_1:40619842-40619861 Msa0207740:intron 40.0%
CTGTGACACATGGTTACTAC+AGG - chr2_1:40616731-40616750 Msa0207740:CDS 45.0%
! GAGGCAAATGATAGTGACAG+TGG - chr2_1:40617244-40617263 Msa0207740:CDS 45.0%
AACCTCTCTGCCTCATTCAT+TGG - chr2_1:40617379-40617398 Msa0207740:CDS 45.0%
ACCTCTCTGCCTCATTCATT+GGG - chr2_1:40617380-40617399 Msa0207740:CDS 45.0%
AATACCCGACCCAATGAATG+AGG + chr2_1:40617392-40617411 None:intergenic 45.0%
TATCACCTGCTAGGTTTAGC+AGG - chr2_1:40617613-40617632 Msa0207740:CDS 45.0%
TACAACAAGTGGTTCCTCCT+GGG + chr2_1:40617954-40617973 None:intergenic 45.0%
TGGGATTTGGGTACAACAAG+TGG + chr2_1:40617965-40617984 None:intergenic 45.0%
! GTAGCCTTAGGTACCAAAGT+TGG + chr2_1:40618021-40618040 None:intergenic 45.0%
TCTGACTTTGTAGCCGACAT+TGG + chr2_1:40618505-40618524 None:intergenic 45.0%
!! AAGAAGATTGGTGATGGTGG+TGG + chr2_1:40618749-40618768 None:intergenic 45.0%
!! TCGAAGAAGATTGGTGATGG+TGG + chr2_1:40618752-40618771 None:intergenic 45.0%
CTGTCGAAGAAGATTGGTGA+TGG + chr2_1:40618755-40618774 None:intergenic 45.0%
TGGAAGATTACCACGTTGCA+CGG + chr2_1:40618841-40618860 None:intergenic 45.0%
GCAACGTGGTAATCTTCCAT+TGG - chr2_1:40618842-40618861 Msa0207740:intron 45.0%
! TCGTTAATCGAAGGCATGCA+AGG - chr2_1:40618870-40618889 Msa0207740:intron 45.0%
! CGTTAATCGAAGGCATGCAA+GGG - chr2_1:40618871-40618890 Msa0207740:intron 45.0%
ATGAACACAATCTCCGGCAT+CGG - chr2_1:40618906-40618925 Msa0207740:intron 45.0%
!! CAAAGCGTCGATGAAGTATC+CGG + chr2_1:40618940-40618959 None:intergenic 45.0%
GACAAGCTTCGTAGTAGTGA+TGG + chr2_1:40619031-40619050 None:intergenic 45.0%
ACCTCATGCATGGTCTTCAA+TGG - chr2_1:40619138-40619157 Msa0207740:intron 45.0%
GCAAGAGCTTGAATTAACGC+TGG + chr2_1:40619164-40619183 None:intergenic 45.0%
AACGTTAGATTCGCCTTTCG+CGG - chr2_1:40619302-40619321 Msa0207740:intron 45.0%
GGCTTGAAGATGTAAAGCCA+TGG - chr2_1:40619339-40619358 Msa0207740:intron 45.0%
TGTAAAGCCATGGATGCTTC+AGG - chr2_1:40619349-40619368 Msa0207740:intron 45.0%
!! GGTGAGTTCTAAAGAAGCTG+TGG - chr2_1:40619370-40619389 Msa0207740:intron 45.0%
!! GAGTTCTAAAGAAGCTGTGG+CGG - chr2_1:40619373-40619392 Msa0207740:intron 45.0%
TTTCAGCTGCATCGTCTTCT+CGG - chr2_1:40619407-40619426 Msa0207740:intron 45.0%
ATCCGACCCGAATTCTCATT+CGG - chr2_1:40619436-40619455 Msa0207740:intron 45.0%
GAAGCGAACCATAACGAGAA+CGG - chr2_1:40619524-40619543 Msa0207740:intron 45.0%
AAGCGAACCATAACGAGAAC+GGG - chr2_1:40619525-40619544 Msa0207740:intron 45.0%
CCATAAACCCGTTCTCGTTA+TGG + chr2_1:40619535-40619554 None:intergenic 45.0%
! CCATAACGAGAACGGGTTTA+TGG - chr2_1:40619532-40619551 Msa0207740:intron 45.0%
! ACGAGAACGGGTTTATGGAA+CGG - chr2_1:40619537-40619556 Msa0207740:intron 45.0%
CGCTTCAAGCGAGTCAAAAA+CGG + chr2_1:40619591-40619610 None:intergenic 45.0%
GGTTGAACCGGATAAAGCTA+TGG - chr2_1:40619616-40619635 Msa0207740:intron 45.0%
!! ATCTCGGCCATAGCTTTATC+CGG + chr2_1:40619626-40619645 None:intergenic 45.0%
AGTTGAGAGACATGAACCGT+TGG - chr2_1:40619697-40619716 Msa0207740:intron 45.0%
! GACATGAACCGTTGGTTAAG+TGG - chr2_1:40619705-40619724 Msa0207740:intron 45.0%
! TGAACCGTTGGTTAAGTGGA+AGG - chr2_1:40619709-40619728 Msa0207740:intron 45.0%
GCATTCGAACCCAAATGCAA+CGG + chr2_1:40619761-40619780 None:intergenic 45.0%
! TTGACTCTGTTTTCAGCGGA+GGG - chr2_1:40619803-40619822 Msa0207740:intron 45.0%
GAATCAAGGGAGCTTGACTT+TGG - chr2_1:40619841-40619860 Msa0207740:intron 45.0%
!! GAGGCAGAGAGGTTGATGAT+GGG + chr2_1:40617373-40617392 None:intergenic 50.0%
!! TGAGGCAGAGAGGTTGATGA+TGG + chr2_1:40617374-40617393 None:intergenic 50.0%
ACCCAATGAATGAGGCAGAG+AGG + chr2_1:40617384-40617403 None:intergenic 50.0%
CTCTGCCTCATTCATTGGGT+CGG - chr2_1:40617384-40617403 Msa0207740:CDS 50.0%
TCTGCCTCATTCATTGGGTC+GGG - chr2_1:40617385-40617404 Msa0207740:CDS 50.0%
!! AAGGTGCTGTCAAGTGTGTC+AGG - chr2_1:40617559-40617578 Msa0207740:CDS 50.0%
! AGGTGCTGTCAAGTGTGTCA+GGG - chr2_1:40617560-40617579 Msa0207740:CDS 50.0%
ATAGTAGTAGACCCAGACCC+AGG - chr2_1:40617934-40617953 Msa0207740:CDS 50.0%
GTACAACAAGTGGTTCCTCC+TGG + chr2_1:40617955-40617974 None:intergenic 50.0%
AGGCACACTAGCGTGACATT+AGG - chr2_1:40618051-40618070 Msa0207740:CDS 50.0%
GGCACACTAGCGTGACATTA+GGG - chr2_1:40618052-40618071 Msa0207740:CDS 50.0%
CTTTGTAGCCGACATTGGCA+AGG + chr2_1:40618500-40618519 None:intergenic 50.0%
CAATGTCGGAGGGATCATAG+TGG + chr2_1:40618626-40618645 None:intergenic 50.0%
CACTATGATCCCTCCGACAT+TGG - chr2_1:40618624-40618643 Msa0207740:intron 50.0%
ATCCCTCCGACATTGGCAAT+TGG - chr2_1:40618631-40618650 Msa0207740:intron 50.0%
TCCCTCCGACATTGGCAATT+GGG - chr2_1:40618632-40618651 Msa0207740:intron 50.0%
ACCCAATTGCCAATGTCGGA+GGG + chr2_1:40618636-40618655 None:intergenic 50.0%
GTCCACCCAATTGCCAATGT+CGG + chr2_1:40618640-40618659 None:intergenic 50.0%
CCTCTCCGAATTTGACCACA+CGG - chr2_1:40618665-40618684 Msa0207740:intron 50.0%
AGAGGCCGTGTGGTCAAATT+CGG + chr2_1:40618673-40618692 None:intergenic 50.0%
CGTGGTAATCTTCCATTGGC+TGG - chr2_1:40618846-40618865 Msa0207740:intron 50.0%
TCGATTAACGAGCCAGCCAA+TGG + chr2_1:40618861-40618880 None:intergenic 50.0%
!! TTGGCTGGCTCGTTAATCGA+AGG - chr2_1:40618861-40618880 Msa0207740:intron 50.0%
! GCTTTGAACCGTCGCATCTT+CGG - chr2_1:40618954-40618973 Msa0207740:intron 50.0%
CGGTGAAGTGTGCGAATTTG+AGG + chr2_1:40619058-40619077 None:intergenic 50.0%
CAATGACGTGGACACAATCG+TGG + chr2_1:40619112-40619131 None:intergenic 50.0%
GCCATTGAAGACCATGCATG+AGG + chr2_1:40619142-40619161 None:intergenic 50.0%
CGATTTCACGGAGGTTGTCA+CGG + chr2_1:40619250-40619269 None:intergenic 50.0%
CGTGACAACCTCCGTGAAAT+CGG - chr2_1:40619248-40619267 Msa0207740:intron 50.0%
! GAACTCACCTGAAGCATCCA+TGG + chr2_1:40619359-40619378 None:intergenic 50.0%
TCCGACCCGAATTCTCATTC+GGG - chr2_1:40619437-40619456 Msa0207740:intron 50.0%
ACCCGAATGAGAATTCGGGT+CGG + chr2_1:40619441-40619460 None:intergenic 50.0%
CGGGTTTATGGAACGGTTCA+CGG - chr2_1:40619544-40619563 Msa0207740:intron 50.0%
GTTTATGGAACGGTTCACGG+AGG - chr2_1:40619547-40619566 Msa0207740:intron 50.0%
TCATGTCTCTCAACTCGAGC+CGG + chr2_1:40619692-40619711 None:intergenic 50.0%
CGCTCCTTCCACTTAACCAA+CGG + chr2_1:40619716-40619735 None:intergenic 50.0%
! CGTTGGTTAAGTGGAAGGAG+CGG - chr2_1:40619714-40619733 Msa0207740:intron 50.0%
CAAATGCAACGGTCTGAACC+CGG + chr2_1:40619750-40619769 None:intergenic 50.0%
GGGTTCAGACCGTTGCATTT+GGG - chr2_1:40619749-40619768 Msa0207740:intron 50.0%
!! GTTGACTCTGTTTTCAGCGG+AGG - chr2_1:40619802-40619821 Msa0207740:intron 50.0%
AAGGGAGCTTGACTTTGGGT+TGG - chr2_1:40619846-40619865 Msa0207740:intron 50.0%
!! TGACTTTGGGTTGGCATAGC+CGG - chr2_1:40619855-40619874 Msa0207740:intron 50.0%
AAGTGGTTCCTCCTGGGTCT+GGG + chr2_1:40617948-40617967 None:intergenic 55.0%
!! AGAGTGGTGGGCTTGAGAGT+GGG + chr2_1:40618276-40618295 None:intergenic 55.0%
!! TAGAGTGGTGGGCTTGAGAG+TGG + chr2_1:40618277-40618296 None:intergenic 55.0%
TGTAGCCGACATTGGCAAGG+AGG + chr2_1:40618497-40618516 None:intergenic 55.0%
CACCCAATTGCCAATGTCGG+AGG + chr2_1:40618637-40618656 None:intergenic 55.0%
CTCCGACATTGGCAATTGGG+TGG - chr2_1:40618635-40618654 Msa0207740:intron 55.0%
CCGTGTGGTCAAATTCGGAG+AGG + chr2_1:40618668-40618687 None:intergenic 55.0%
!! TGATGGTGGTGGTGTTGTGG+CGG + chr2_1:40618738-40618757 None:intergenic 55.0%
!! TGGTGATGGTGGTGGTGTTG+TGG + chr2_1:40618741-40618760 None:intergenic 55.0%
CTGCACAGGTCATCAGCATG+TGG + chr2_1:40618815-40618834 None:intergenic 55.0%
ACGTTGCACGGAATCTGCAC+AGG + chr2_1:40618829-40618848 None:intergenic 55.0%
TGTGCAGATTCCGTGCAACG+TGG - chr2_1:40618828-40618847 Msa0207740:intron 55.0%
CAAGCTCTTGCTCTTCGTCC+TGG - chr2_1:40619173-40619192 Msa0207740:intron 55.0%
ATGCCGGTAAGCCGGAGAAA+CGG + chr2_1:40619206-40619225 None:intergenic 55.0%
TTTCTCCGGCTTACCGGCAT+TGG - chr2_1:40619206-40619225 Msa0207740:intron 55.0%
TTGTCACGGTCATCCAGCGA+CGG + chr2_1:40619236-40619255 None:intergenic 55.0%
AGCGTGCAAGTTCAGCGAGA+CGG + chr2_1:40619277-40619296 None:intergenic 55.0%
TGACTCGCTTGAAGCGTGTC+CGG - chr2_1:40619595-40619614 Msa0207740:intron 55.0%
TGAAGCGTGTCCGGTTGAAC+CGG - chr2_1:40619604-40619623 Msa0207740:intron 55.0%
ATGCAACGTAGTGTGCTGCG+AGG - chr2_1:40619664-40619683 Msa0207740:intron 55.0%
TGCAACGTAGTGTGCTGCGA+GGG - chr2_1:40619665-40619684 Msa0207740:intron 55.0%
GTTAAGTGGAAGGAGCGGCT+CGG - chr2_1:40619719-40619738 Msa0207740:intron 55.0%
CGGGTTCAGACCGTTGCATT+TGG - chr2_1:40619748-40619767 Msa0207740:intron 55.0%
TTCAGCGGAGGGTTATTGCG+TGG - chr2_1:40619814-40619833 Msa0207740:intron 55.0%
GCATAGCCGGCCTTTGATTG+CGG - chr2_1:40619868-40619887 Msa0207740:intron 55.0%
CAAGCCGAAGCCGCAATCAA+AGG + chr2_1:40619881-40619900 None:intergenic 55.0%
CTTTGATTGCGGCTTCGGCT+TGG - chr2_1:40619879-40619898 Msa0207740:intron 55.0%
GTAGTAGACCCAGACCCAGG+AGG - chr2_1:40617937-40617956 Msa0207740:CDS 60.0%
CAAGTGGTTCCTCCTGGGTC+TGG + chr2_1:40617949-40617968 None:intergenic 60.0%
GACGGCCTCCTTGCCAATGT+CGG - chr2_1:40618489-40618508 Msa0207740:intron 60.0%
CGTAGGGTAAAGAGGCCGTG+TGG + chr2_1:40618683-40618702 None:intergenic 60.0%
! TCCGGCATCGGAAAAGTCGC+CGG - chr2_1:40618918-40618937 Msa0207740:intron 60.0%
! TCCGGCGACTTTTCCGATGC+CGG + chr2_1:40618922-40618941 None:intergenic 60.0%
GCTCTTGCTCTTCGTCCTGG+CGG - chr2_1:40619176-40619195 Msa0207740:intron 60.0%
CCTCCGTTTCTCCGGCTTAC+CGG - chr2_1:40619200-40619219 Msa0207740:intron 60.0%
! CGAGACGGAGGCCGATTTCA+CGG + chr2_1:40619262-40619281 None:intergenic 60.0%
GTGCAAGTTCAGCGAGACGG+AGG + chr2_1:40619274-40619293 None:intergenic 60.0%
ATTCGCCTTTCGCGGCGTTG+CGG - chr2_1:40619310-40619329 Msa0207740:intron 60.0%
!! AAGGAGCGGCTCGGTAAAGC+CGG - chr2_1:40619728-40619747 Msa0207740:intron 60.0%
CCGAAGCCGCAATCAAAGGC+CGG + chr2_1:40619877-40619896 None:intergenic 60.0%
CCGGCCTTTGATTGCGGCTT+CGG - chr2_1:40619874-40619893 Msa0207740:intron 60.0%
GGCGGCTGTGCTGAGAGATC+TGG + chr2_1:40618720-40618739 None:intergenic 65.0%
! CTGGCGGACCTCCGTTTCTC+CGG - chr2_1:40619192-40619211 Msa0207740:intron 65.0%
CCGGTAAGCCGGAGAAACGG+AGG + chr2_1:40619203-40619222 None:intergenic 65.0%
GCGGACCAATGCCGGTAAGC+CGG + chr2_1:40619214-40619233 None:intergenic 65.0%
TCACGGTCATCCAGCGACGG+CGG + chr2_1:40619233-40619252 None:intergenic 65.0%
! GACGGAGGCCGATTTCACGG+AGG + chr2_1:40619259-40619278 None:intergenic 65.0%
CGTAGTGTGCTGCGAGGGAC+CGG - chr2_1:40619670-40619689 Msa0207740:intron 65.0%
!! AGGAGCGGCTCGGTAAAGCC+GGG - chr2_1:40619729-40619748 Msa0207740:intron 65.0%
CGGAGAAACGGAGGTCCGCC+AGG + chr2_1:40619194-40619213 None:intergenic 70.0%
CAGCGACGGCGGACCAATGC+CGG + chr2_1:40619222-40619241 None:intergenic 70.0%
!! CGGCATTGGTCCGCCGTCGC+TGG - chr2_1:40619220-40619239 Msa0207740:intron 75.0%
CCACGCCGCAACGCCGCGAA+AGG + chr2_1:40619318-40619337 None:intergenic 75.0%
CCTTTCGCGGCGTTGCGGCG+TGG - chr2_1:40619315-40619334 Msa0207740:intron 75.0%
TTCGCGGCGTTGCGGCGTGG+CGG - chr2_1:40619318-40619337 Msa0207740:intron 75.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 40616676 40619949 40616676 ID=Msa0207740;Name=Msa0207740
chr2_1 mRNA 40616676 40619949 40616676 ID=Msa0207740-mRNA-1;Parent=Msa0207740;Name=Msa0207740-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|0|0|0.33|1|1|3|0|500
chr2_1 exon 40619946 40619949 40619946 ID=Msa0207740-mRNA-1:exon:12519;Parent=Msa0207740-mRNA-1
chr2_1 exon 40618781 40618786 40618781 ID=Msa0207740-mRNA-1:exon:12518;Parent=Msa0207740-mRNA-1
chr2_1 exon 40616676 40618168 40616676 ID=Msa0207740-mRNA-1:exon:12517;Parent=Msa0207740-mRNA-1
chr2_1 CDS 40619946 40619949 40619946 ID=Msa0207740-mRNA-1:cds;Parent=Msa0207740-mRNA-1
chr2_1 CDS 40618781 40618786 40618781 ID=Msa0207740-mRNA-1:cds;Parent=Msa0207740-mRNA-1
chr2_1 CDS 40616676 40618168 40616676 ID=Msa0207740-mRNA-1:cds;Parent=Msa0207740-mRNA-1
Gene Sequence

>Msa0207740

ATGGTTTGTGATTCTTCTTCCCCATCAAAGACAAAAGACATCGACGGCCTCCTTGCCAATGTCGGCTACAAAGTCAGATCATCTGAGCTACATCAAGTAGCTCAGAATCTCGAACGATTAGAAAGCGCGATTGTGAACTCATCGAGCGACATTTCTCAATTCGCTTCGGATACAGTCCACTATGATCCCTCCGACATTGGCAATTGGGTGGACAATCTCCTCTCCGAATTTGACCACACGGCCTCTTTACCCTACGATTTCTCTGAACTTCCAGATCTCTCAGCACAGCCGCCACAACACCACCACCATCACCAATCTTCTTCGACAGTTAATACTGTCGAAGAAGATTCTGCAATAAAACTCGTCCACATGCTGATGACCTGTGCAGATTCCGTGCAACGTGGTAATCTTCCATTGGCTGGCTCGTTAATCGAAGGCATGCAAGGGTTGTTAGCAAACATGAACACAATCTCCGGCATCGGAAAAGTCGCCGGATACTTCATCGACGCTTTGAACCGTCGCATCTTCGGTCAAAATAATGTTAGCCACCAAGTTTCTCTTTACGAGAACGATGTTCTTTACCATCACTACTACGAAGCTTGTCCATACCTCAAATTCGCACACTTCACCGCAAATCAAGCTATACTCGAAGCTTTCAACGGCCACGATTGTGTCCACGTCATTGATTTCAACCTCATGCATGGTCTTCAATGGCCAGCGTTAATTCAAGCTCTTGCTCTTCGTCCTGGCGGACCTCCGTTTCTCCGGCTTACCGGCATTGGTCCGCCGTCGCTGGATGACCGTGACAACCTCCGTGAAATCGGCCTCCGTCTCGCTGAACTTGCACGCTCCGTTAACGTTAGATTCGCCTTTCGCGGCGTTGCGGCGTGGCGGCTTGAAGATGTAAAGCCATGGATGCTTCAGGTGAGTTCTAAAGAAGCTGTGGCGGTTAATTCTATTTTTCAGCTGCATCGTCTTCTCGGATCTGAATCCGACCCGAATTCTCATTCGGGTATTGAAATGGTTTTGGGTTGGATCCGGAGTTTGAATCCGAAAATCGTTACAGTTGTTGAACAAGAAGCGAACCATAACGAGAACGGGTTTATGGAACGGTTCACGGAGGCATTGCATTATTACTCGACCGTTTTTGACTCGCTTGAAGCGTGTCCGGTTGAACCGGATAAAGCTATGGCCGAGATGTATTTACAAAGAGAGATATGCAACGTAGTGTGCTGCGAGGGACCGGCTCGAGTTGAGAGACATGAACCGTTGGTTAAGTGGAAGGAGCGGCTCGGTAAAGCCGGGTTCAGACCGTTGCATTTGGGTTCGAATGCTTTTAAGCAAGCGAGTATGTTGTTGACTCTGTTTTCAGCGGAGGGTTATTGCGTGGAAGAGAATCAAGGGAGCTTGACTTTGGGTTGGCATAGCCGGCCTTTGATTGCGGCTTCGGCTTGGCAAGCTGTGCCTTTGTTAGATGCTGAAACACTGCGTTTTGATTGTTGA
Protein sequence

>Msa0207740

MVCDSSSPSKTKDIDGLLANVGYKVRSSELHQVAQNLERLESAIVNSSSDISQFASDTVHYDPSDIGNWVDNLLSEFDHTASLPYDFSELPDLSAQPPQHHHHHQSSSTVNTVEEDSAIKLVHMLMTCADSVQRGNLPLAGSLIEGMQGLLANMNTISGIGKVAGYFIDALNRRIFGQNNVSHQVSLYENDVLYHHYYEACPYLKFAHFTANQAILEAFNGHDCVHVIDFNLMHGLQWPALIQALALRPGGPPFLRLTGIGPPSLDDRDNLREIGLRLAELARSVNVRFAFRGVAAWRLEDVKPWMLQVSSKEAVAVNSIFQLHRLLGSESDPNSHSGIEMVLGWIRSLNPKIVTVVEQEANHNENGFMERFTEALHYYSTVFDSLEACPVEPDKAMAEMYLQREICNVVCCEGPARVERHEPLVKWKERLGKAGFRPLHLGSNAFKQASMLLTLFSAEGYCVEENQGSLTLGWHSRPLIAASAWQAVPLLDAETLRFDC*