Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0207920 | XP_039687084.1 | 84.426 | 122 | 17 | 1 | 1 | 120 | 1 | 122 | 6.99e-69 | 213 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0207920 | G7INX6 | 84.426 | 122 | 17 | 1 | 1 | 120 | 1 | 122 | 3.34e-69 | 213 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0207920 | TF | B3 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0207920 | MtrunA17_Chr2g0281511 | 84.426 | 122 | 17 | 1 | 1 | 120 | 1 | 122 | 6.43e-73 | 213 |
| Msa0207920 | MtrunA17_Chr2g0309121 | 81.148 | 122 | 20 | 2 | 1 | 120 | 1 | 121 | 1.41e-67 | 199 |
| Msa0207920 | MtrunA17_Chr3g0081891 | 81.250 | 80 | 13 | 1 | 1 | 78 | 1 | 80 | 1.73e-42 | 134 |
| Msa0207920 | MtrunA17_Chr7g0276661 | 35.878 | 131 | 62 | 5 | 7 | 117 | 38 | 166 | 5.90e-20 | 80.1 |
| Msa0207920 | MtrunA17_Chr5g0430431 | 48.214 | 56 | 29 | 0 | 59 | 114 | 199 | 254 | 3.27e-13 | 63.9 |
| Msa0207920 | MtrunA17_Chr0c01g0489371 | 39.560 | 91 | 51 | 2 | 22 | 108 | 224 | 314 | 5.65e-13 | 63.5 |
| Msa0207920 | MtrunA17_Chr8g0372431 | 27.835 | 97 | 68 | 1 | 24 | 118 | 6 | 102 | 8.54e-13 | 60.1 |
| Msa0207920 | MtrunA17_Chr8g0356961 | 36.170 | 94 | 56 | 2 | 19 | 108 | 72 | 165 | 3.02e-12 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAATGTTACTGCTTCTATTT+TGG | 0.123735 | 2_1:-40944911 | Msa0207920:CDS |
| GAAGTTAATGGTGCTGAATT+AGG | 0.163350 | 2_1:-40944970 | Msa0207920:CDS |
| AAAATTCCGATGTTCATTAT+TGG | 0.199581 | 2_1:-40944939 | Msa0207920:CDS |
| TGTATGCTACCGTCGTTAAC+TGG | 0.297739 | 2_1:-40944550 | Msa0207920:CDS |
| GAGATTGGAGTTGAAGTTAA+TGG | 0.344431 | 2_1:-40944982 | Msa0207920:CDS |
| AAGTTAATGGTGCTGAATTA+GGG | 0.367629 | 2_1:-40944969 | Msa0207920:CDS |
| CAGTCAATAATCCTCCTAAT+AGG | 0.398760 | 2_1:-40944574 | Msa0207920:CDS |
| TTACCAGTTTCTTCATCAAA+AGG | 0.404700 | 2_1:+40944716 | None:intergenic |
| CCTCGACGTATTGCTGAGAA+TGG | 0.434646 | 2_1:-40944767 | Msa0207920:CDS |
| ACGGTAGCATACATCCTATT+AGG | 0.440754 | 2_1:+40944560 | None:intergenic |
| TACCAGTTTCTTCATCAAAA+GGG | 0.488019 | 2_1:+40944717 | None:intergenic |
| GTATGCTACCGTCGTTAACT+GGG | 0.522932 | 2_1:-40944549 | Msa0207920:CDS |
| AGCATGCATCAGAAATACAT+AGG | 0.564126 | 2_1:-40944659 | Msa0207920:CDS |
| CTTGAAAAGTGCCTAAAAGT+TGG | 0.570138 | 2_1:-40944614 | Msa0207920:CDS |
| AGAAATACATAGGGAGAGGA+TGG | 0.573439 | 2_1:-40944649 | Msa0207920:CDS |
| CATCAGAAATACATAGGGAG+AGG | 0.580360 | 2_1:-40944653 | Msa0207920:CDS |
| GCATGCATCAGAAATACATA+GGG | 0.596673 | 2_1:-40944658 | Msa0207920:CDS |
| GTAGCATACATCCTATTAGG+AGG | 0.633251 | 2_1:+40944563 | None:intergenic |
| ATACTTCCAATAATGAACAT+CGG | 0.646998 | 2_1:+40944933 | None:intergenic |
| CCATTCTCAGCAATACGTCG+AGG | 0.710417 | 2_1:+40944767 | None:intergenic |
| GAATCAGTCCCAGTTAACGA+CGG | 0.733495 | 2_1:+40944541 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAATTCCGATGTTCATTAT+TGG | - | chr2_1:40944593-40944612 | Msa0207920:CDS | 25.0% |
| ! | ATACTTCCAATAATGAACAT+CGG | + | chr2_1:40944602-40944621 | None:intergenic | 25.0% |
| !! | TAATGTTACTGCTTCTATTT+TGG | - | chr2_1:40944621-40944640 | Msa0207920:CDS | 25.0% |
| ! | AAGTTAATGGTGCTGAATTA+GGG | - | chr2_1:40944563-40944582 | Msa0207920:CDS | 30.0% |
| ! | TTTTGGATGGAAACAATGTT+TGG | - | chr2_1:40944638-40944657 | Msa0207920:CDS | 30.0% |
| TACCAGTTTCTTCATCAAAA+GGG | + | chr2_1:40944818-40944837 | None:intergenic | 30.0% | |
| TTACCAGTTTCTTCATCAAA+AGG | + | chr2_1:40944819-40944838 | None:intergenic | 30.0% | |
| ! | AAATAACGTCACCAACTTTT+AGG | + | chr2_1:40944932-40944951 | None:intergenic | 30.0% |
| GAGATTGGAGTTGAAGTTAA+TGG | - | chr2_1:40944550-40944569 | Msa0207920:CDS | 35.0% | |
| ! | GAAGTTAATGGTGCTGAATT+AGG | - | chr2_1:40944562-40944581 | Msa0207920:CDS | 35.0% |
| !! | GTTACTGCTTCTATTTTGGA+TGG | - | chr2_1:40944625-40944644 | Msa0207920:CDS | 35.0% |
| TGGAAACAATGTTTGGTTAG+TGG | - | chr2_1:40944645-40944664 | Msa0207920:CDS | 35.0% | |
| AGCATGCATCAGAAATACAT+AGG | - | chr2_1:40944873-40944892 | Msa0207920:intron | 35.0% | |
| GCATGCATCAGAAATACATA+GGG | - | chr2_1:40944874-40944893 | Msa0207920:intron | 35.0% | |
| !! | CTTGAAAAGTGCCTAAAAGT+TGG | - | chr2_1:40944918-40944937 | Msa0207920:CDS | 35.0% |
| CAGTCAATAATCCTCCTAAT+AGG | - | chr2_1:40944958-40944977 | Msa0207920:CDS | 35.0% | |
| ! | CTCCCTTTTGATGAAGAAAC+TGG | - | chr2_1:40944813-40944832 | Msa0207920:intron | 40.0% |
| CATCAGAAATACATAGGGAG+AGG | - | chr2_1:40944879-40944898 | Msa0207920:intron | 40.0% | |
| AGAAATACATAGGGAGAGGA+TGG | - | chr2_1:40944883-40944902 | Msa0207920:intron | 40.0% | |
| GTAGCATACATCCTATTAGG+AGG | + | chr2_1:40944972-40944991 | None:intergenic | 40.0% | |
| ACGGTAGCATACATCCTATT+AGG | + | chr2_1:40944975-40944994 | None:intergenic | 40.0% | |
| TGTATGCTACCGTCGTTAAC+TGG | - | chr2_1:40944982-40945001 | Msa0207920:CDS | 45.0% | |
| GTATGCTACCGTCGTTAACT+GGG | - | chr2_1:40944983-40945002 | Msa0207920:CDS | 45.0% | |
| CCATTCTCAGCAATACGTCG+AGG | + | chr2_1:40944768-40944787 | None:intergenic | 50.0% | |
| CCTCGACGTATTGCTGAGAA+TGG | - | chr2_1:40944765-40944784 | Msa0207920:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_1 | gene | 40944544 | 40945010 | 40944544 | ID=Msa0207920;Name=Msa0207920 |
| chr2_1 | mRNA | 40944544 | 40945010 | 40944544 | ID=Msa0207920-mRNA-1;Parent=Msa0207920;Name=Msa0207920-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|2|0|120 |
| chr2_1 | exon | 40944900 | 40945010 | 40944900 | ID=Msa0207920-mRNA-1:exon:12609;Parent=Msa0207920-mRNA-1 |
| chr2_1 | exon | 40944544 | 40944795 | 40944544 | ID=Msa0207920-mRNA-1:exon:12608;Parent=Msa0207920-mRNA-1 |
| chr2_1 | CDS | 40944900 | 40945010 | 40944900 | ID=Msa0207920-mRNA-1:cds;Parent=Msa0207920-mRNA-1 |
| chr2_1 | CDS | 40944544 | 40944795 | 40944544 | ID=Msa0207920-mRNA-1:cds;Parent=Msa0207920-mRNA-1 |
| Gene Sequence |
| Protein sequence |
