AlfalfaGEDB | Msa0210140

M. sativa cultivar zhongmu-4 / Msa0210140

KEGG: -
GO: -
PFAM: PF13302.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0210140	XP_003610737.1	94.186	172	9	1	1	171	19	190	7.08e-112	326

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0210140	sp\|P05332\|YP20_BACLI	32.934	167	107	3	3	166	8	172	9.54e-17	76.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0210140	G7K3P2	94.186	172	9	1	1	171	19	190	3.38e-112	326

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TFs/TRs:

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Gene ID	Type	Classification
Msa0210140	TR	GNAT

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0210140	MtrunA17_Chr5g0394621	94.186	172	9	1	1	171	19	190	6.51e-116	326
Msa0210140	MtrunA17_Chr5g0394631	79.630	162	33	0	1	162	19	180	2.95e-88	263
Msa0210140	MtrunA17_Chr5g0394631	74.432	176	38	5	1	171	228	401	1.34e-80	244
Msa0210140	MtrunA17_Chr1g0204951	67.665	167	52	1	1	167	21	185	1.56e-79	234
Msa0210140	MtrunA17_Chr1g0204971	64.072	167	58	1	1	167	21	185	7.92e-75	222
Msa0210140	MtrunA17_Chr7g0273661	40.909	154	88	1	1	154	6	156	1.90e-39	131
Msa0210140	MtrunA17_Chr7g0223351	37.952	166	98	3	2	167	7	167	4.29e-34	118
Msa0210140	MtrunA17_Chr7g0223361	37.576	165	99	2	2	166	7	167	6.98e-34	117

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0210140	AT2G32030.1	51.515	165	73	3	3	166	24	182	2.61e-56	175
Msa0210140	AT2G32020.1	50.000	166	76	2	3	167	19	178	3.30e-52	165
Msa0210140	AT3G22560.1	39.053	169	98	2	2	168	6	171	4.03e-36	124

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Find 43 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGGGGTCGGAGTGTGATTT+GGG	0.387621	2_1:+50069707	Msa0210130:intron
AAGTGGCCAAGTTCTGCTCT+TGG	0.474319	2_1:-50069638	Msa0210140:CDS
ACCTTGATGATCTCATGGTA+TGG	0.538119	2_1:-50069674	Msa0210140:CDS
AGGAAATATTTGTTCATGAA+AGG	0.480337	2_1:-50069270	Msa0210140:CDS
AGGAACAAATGTGCAGAACT+TGG	0.481600	2_1:-50069474	Msa0210140:CDS
AGTGGCCAAGTTCTGCTCTT+GGG	0.462852	2_1:-50069637	Msa0210140:CDS
ATATAGCTACCAAGTTTCTA+TGG	0.402105	2_1:-50069572	Msa0210140:CDS
ATCAAGGTCAGAAAGATTAA+GGG	0.492658	2_1:+50069689	Msa0210130:intron
ATGTTCTAGGCTATAAATAT+TGG	0.289347	2_1:-50069449	Msa0210140:CDS
ATTCTGTGAGTTGTCTTACT+TGG	0.446468	2_1:-50069379	Msa0210140:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATCAAGGTCAGAAAGATTAA+GGG	+	chr2_1:50069243-50069262	Msa0210130:intron	30.0%
ATATAGCTACCAAGTTTCTA+TGG	-	chr2_1:50069357-50069376	Msa0210140:CDS	30.0%
GCTATAAATATTGGGGTAAA+GGG	-	chr2_1:50069489-50069508	Msa0210140:CDS	30.0%
CTATAAATATTGGGGTAAAG+GGG	-	chr2_1:50069490-50069509	Msa0210140:CDS	30.0%
TCAAGGTCAGAAAGATTAAG+GGG	+	chr2_1:50069242-50069261	Msa0210130:intron	35.0%
CATCAAGGTCAGAAAGATTA+AGG	+	chr2_1:50069244-50069263	Msa0210130:intron	35.0%
ATGGACAACTGATGAAAAAG+TGG	-	chr2_1:50069274-50069293	Msa0210140:CDS	35.0%
TTGTTCCTTGATTTATCACC+AGG	+	chr2_1:50069443-50069462	Msa0210130:intron	35.0%
GGCTATAAATATTGGGGTAA+AGG	-	chr2_1:50069488-50069507	Msa0210140:CDS	35.0%
TGTTGTGAAACAAGAGGTTA+AGG	-	chr2_1:50069523-50069542	Msa0210140:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	50069218	50069733	50069218	ID=Msa0210140;Name=Msa0210140
chr2_1	mRNA	50069218	50069733	50069218	ID=Msa0210140-mRNA-1;Parent=Msa0210140;Name=Msa0210140-mRNA-1;_AED=0.27;_eAED=0.27;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|171
chr2_1	exon	50069218	50069733	50069218	ID=Msa0210140-mRNA-1:exon:13835;Parent=Msa0210140-mRNA-1
chr2_1	CDS	50069218	50069733	50069218	ID=Msa0210140-mRNA-1:cds;Parent=Msa0210140-mRNA-1

Gene Sequence

>Msa0210140

CTGACCCAAATCACACTCCGACCCCTTAATCTTTCTGACCTTGATGATCTCATGGTATGGACAACTGATGAAAAAGTGGCCAAGTTCTGCTCTTGGGAACTCTACACCAGCAAGGATGATGGCATCAACTTCATTGAAAATATAGCTACCAAGTTTCTATGGTGCAAAGCAATATGCATCAATGATCGTGCAATTGAGTGTGTTTCTCTATCCTCAAGTTCACCTGGTGATAAATCAAGGAACAAATGTGCAGAACTTGGCTATGTTCTAGGCTATAAATATTGGGGTAAAGGGGTTGCCACGTGTGTTGTGAAACAAGAGGTTAAGGCTGCATTCTGTGAGTTGTCTTACTTGGAAAGGCTTGAAGCTTTAGTTGATGTGGAAAATGTTGGGTCTCAAAGAGTTTTGGAGAAAGCTGGTTTCCAAAAAGAGGGAGTTCTAAGGAAATATTTGTTCATGAAAGGAAAAAGTAGAGATATGATCATGTCCAGTGTTCTTACTGATCTCCAACTATAA

Protein sequence

>Msa0210140

LTQITLRPLNLSDLDDLMVWTTDEKVAKFCSWELYTSKDDGINFIENIATKFLWCKAICINDRAIECVSLSSSSPGDKSRNKCAELGYVLGYKYWGKGVATCVVKQEVKAAFCELSYLERLEALVDVENVGSQRVLEKAGFQKEGVLRKYLFMKGKSRDMIMSSVLTDLQL*