AlfalfaGEDB | Msa0211060

M. sativa cultivar zhongmu-4 / Msa0211060

KEGG: K09422
GO: GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031326 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0211060	XP_003595957.1	94.091	220	8	1	1	215	1	220	1.20e-140	412

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0211060	sp\|Q8LPH6\|MYB86_ARATH	72.093	129	35	1	1	128	1	129	2.12e-65	214

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0211060	G7IGW8	94.091	220	8	1	1	215	1	220	5.72e-141	412

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TFs/TRs:

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Gene ID	Type	Classification
Msa0211060	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0211060	MtrunA17_Chr2g0309911	94.091	220	8	1	1	215	1	220	1.10e-144	412
Msa0211060	MtrunA17_Chr2g0330041	55.140	214	77	5	1	201	1	208	8.69e-69	221
Msa0211060	MtrunA17_Chr8g0342521	52.444	225	80	8	1	214	1	209	1.63e-67	216
Msa0211060	MtrunA17_Chr1g0193371	70.769	130	37	1	1	129	1	130	6.63e-67	216
Msa0211060	MtrunA17_Chr8g0342541	52.252	222	89	6	1	214	1	213	1.07e-66	214
Msa0211060	MtrunA17_Chr4g0058631	50.476	210	84	3	1	190	1	210	2.01e-66	214
Msa0211060	MtrunA17_Chr1g0211161	54.211	190	83	3	3	191	5	191	1.36e-65	207
Msa0211060	MtrunA17_Chr7g0270891	70.769	130	37	1	1	129	1	130	2.87e-65	211
Msa0211060	MtrunA17_Chr1g0193801	69.231	130	39	1	1	129	1	130	2.30e-64	209
Msa0211060	MtrunA17_Chr3g0117091	72.222	126	34	1	1	125	1	126	7.03e-64	207

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0211060	AT3G12720.1	57.269	227	73	4	1	205	12	236	9.86e-84	257
Msa0211060	AT1G57560.1	65.278	144	50	0	3	146	4	147	1.75e-68	218
Msa0211060	AT1G57560.2	61.635	159	49	2	3	158	4	153	6.88e-68	215
Msa0211060	AT5G26660.1	72.093	129	35	1	1	128	1	129	2.15e-66	214
Msa0211060	AT4G01680.3	70.769	130	37	1	1	129	1	130	5.74e-66	209
Msa0211060	AT3G13890.2	79.661	118	23	1	1	117	1	118	9.12e-66	213
Msa0211060	AT1G09540.1	70.769	130	37	1	1	129	1	130	1.15e-65	213
Msa0211060	AT4G01680.1	70.769	130	37	1	1	129	1	130	3.85e-65	210
Msa0211060	AT3G13890.1	74.016	127	23	2	1	117	1	127	1.42e-62	205
Msa0211060	AT4G01680.2	64.789	142	37	2	1	129	1	142	1.46e-61	201

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Find 78 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTATACCGACGTCCACG+TGG	0.644776	2_1:+51810379	Msa0211060:CDS
AAAGATGTTTGATATTGGTT+AGG	0.341628	2_1:-51809559	None:intergenic
AAAGTTATCAACCACTATCT+TGG	0.379421	2_1:-51810645	None:intergenic
AAATTTATACCTGCAAATTT+TGG	0.157038	2_1:-51808025	None:intergenic
AAATTTGAGTTGTTTCTCTT+AGG	0.336904	2_1:-51809409	None:intergenic
AACTCTTTCCACATCTTTGC+AGG	0.257084	2_1:-51808701	None:intergenic
AAGATGTTTGATATTGGTTA+GGG	0.398808	2_1:-51809558	None:intergenic
AATCGTAAGGTTGCGAAGAG+AGG	0.574715	2_1:+51810334	Msa0211060:CDS
ACAAGCTTATCATCTTCTTC+AGG	0.367541	2_1:-51807959	None:intergenic
ACAGATTGTTTCTCCCGTCT+CGG	0.560472	2_1:+51810530	Msa0211060:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAAGATCAAAAGAGGATTA+TGG	+	chr2_1:51807933-51807952	Msa0211060:CDS	30.0%
GTAAGAGTCAGATATATTTG+TGG	+	chr2_1:51808197-51808216	Msa0211060:intron	30.0%
CAGCAATAGAAGAAAAACTT+AGG	-	chr2_1:51808302-51808321	None:intergenic	30.0%
AAAAAATATGATGCTAGCTG+TGG	+	chr2_1:51808663-51808682	Msa0211060:intron	30.0%
TACATAGAATTCTTGGAAAC+AGG	+	chr2_1:51808808-51808827	Msa0211060:CDS	30.0%
AGACACTTAAAAATTGTGAG+AGG	+	chr2_1:51809053-51809072	Msa0211060:intron	30.0%
AAAATTGTGAGAGGTCAAAT+AGG	+	chr2_1:51809062-51809081	Msa0211060:intron	30.0%
GAAAGTAAATTGTGAGTTTG+TGG	-	chr2_1:51809379-51809398	None:intergenic	30.0%
TATAGTTCATACTTCATTGG+AGG	+	chr2_1:51809826-51809845	Msa0211060:intron	30.0%
TTGGAGGTAATGAATAAGTT+TGG	+	chr2_1:51809842-51809861	Msa0211060:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	51807908	51810805	51807908	ID=Msa0211060;Name=Msa0211060
chr2_1	mRNA	51807908	51810805	51807908	ID=Msa0211060-mRNA-1;Parent=Msa0211060;Name=Msa0211060-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0\|0\|0\|0.75\|1\|1\|4\|0\|373
chr2_1	exon	51807908	51808037	51807908	ID=Msa0211060-mRNA-1:exon:14393;Parent=Msa0211060-mRNA-1
chr2_1	exon	51808700	51808829	51808700	ID=Msa0211060-mRNA-1:exon:14394;Parent=Msa0211060-mRNA-1
chr2_1	exon	51809273	51809658	51809273	ID=Msa0211060-mRNA-1:exon:14395;Parent=Msa0211060-mRNA-1
chr2_1	exon	51810330	51810805	51810330	ID=Msa0211060-mRNA-1:exon:14396;Parent=Msa0211060-mRNA-1
chr2_1	CDS	51807908	51808037	51807908	ID=Msa0211060-mRNA-1:cds;Parent=Msa0211060-mRNA-1
chr2_1	CDS	51808700	51808829	51808700	ID=Msa0211060-mRNA-1:cds;Parent=Msa0211060-mRNA-1
chr2_1	CDS	51809273	51809658	51809273	ID=Msa0211060-mRNA-1:cds;Parent=Msa0211060-mRNA-1
chr2_1	CDS	51810330	51810805	51810330	ID=Msa0211060-mRNA-1:cds;Parent=Msa0211060-mRNA-1

Gene Sequence

>Msa0211060

ATGGGCCATCATTGTTGCAGCAAGCAGAAGATCAAAAGAGGATTATGGTCTCCTGAAGAAGATGATAAGCTTGTTACATATATCACCACTCATGGACATAAAAGCTGGAGTTCTGTTCCAAAATTTGCAGGCCTGCAAAGATGTGGAAAGAGTTGCAGATTAAGATGGACAAACTATTTGAGACCAGATCTAAAAAGAGGTTCCTTCACTGCTGAAGAAGAGCAGATCATAATTGACATACATAGAATTCTTGGAAACAGATGGGCCCAAATAGCAAAGCACCTTCCTGGTAGAACTGACAATGAAGTGAAGAATTTTTGGAACTCTTGCATTAAAAAGAAGCTTATTTCTCAAGGCTTAGATCCACAAACTCACAATTTACTTTCTTCTTCTCATCCTAAGAGAAACAACTCAAATTTTCAAAGTTCCAATTCCATTTTCATTCTTAGTTCACAAATGCCACCAAATACTAATTATCTCATTGAAACAACAAATCAAACTTTTCCTTCACAAAATAATATTCTTCAAACTCCTACAAATGTTTCCCCTAACCAATATCAAACATCTTTCAATGTACTTTCAAACCAACTCGTTGTTCCCGAGCACGAACTGCCTCGAAAATTAACTGAGAACATATCAAACATTTCCCAAATCGTAAGGTTGCGAAGAGAGGATGCCCCGTTGACGCATCAAAGAAACTATACCGACGTCCACGTGGTTGAATGGTCGAAACCGGCTCATGGCAGGTACAAATGTAATATTGACGCCTCTTTCTCCTCTTCCATGAATATGGTGGGTATAGGTATTTGTCTTCGTGATGAAAGGGGCGAATTTATTAGTGCTAAAACAGATTGTTTCTCCCGTCTCGGCGATGTTGCTGTGGGTGAGGCCCTAGCTTTTCATACGGCGTTGAAGTGGATGGCAGATCTTCACTATGATAATGTTGACTTTGCTTTAGATTCCAAGATAGTGGTTGATAACTTTCGATCTTATGTAGTCCTAGTGAGCTTGGATGTATCATGCATGCTTGTAGACAGTTGTTTGACAATAGTTTTCAGAATTCTCATGTTGAGTTCAATAGGAGGCAAGCCAATGGGGTCGCTCATGAGTTACATAGGGTAG

Protein sequence

>Msa0211060

MGHHCCSKQKIKRGLWSPEEDDKLVTYITTHGHKSWSSVPKFAGLQRCGKSCRLRWTNYLRPDLKRGSFTAEEEQIIIDIHRILGNRWAQIAKHLPGRTDNEVKNFWNSCIKKKLISQGLDPQTHNLLSSSHPKRNNSNFQSSNSIFILSSQMPPNTNYLIETTNQTFPSQNNILQTPTNVSPNQYQTSFNVLSNQLVVPEHELPRKLTENISNISQIVRLRREDAPLTHQRNYTDVHVVEWSKPAHGRYKCNIDASFSSSMNMVGIGICLRDERGEFISAKTDCFSRLGDVAVGEALAFHTALKWMADLHYDNVDFALDSKIVVDNFRSYVVLVSLDVSCMLVDSCLTIVFRILMLSSIGGKPMGSLMSYIG*