AlfalfaGEDB | Msa0212460

M. sativa cultivar zhongmu-4 / Msa0212460

KEGG: -
GO: GO:0003674 GO:0003700 GO:0005975 GO:0005984 GO:0005991 GO:0005992 GO:0006082 GO:0006355 GO:0006520 GO:0006560 GO:0006561 GO:0006725 GO:0006790 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009064 GO:0009084 GO:0009311 GO:0009312 GO:0009403 GO:0009404 GO:0009628 GO:0009651 GO:0009700 GO:0009718 GO:0009812 GO:0009813 GO:0009889 GO:0009987 GO:0010120 GO:0010150 GO:0010468 GO:0010556 GO:0016051 GO:0016053 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019748 GO:0019752 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0034637 GO:0034641 GO:0042430 GO:0042435 GO:0042440 GO:0042538 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044262 GO:0044271 GO:0044272 GO:0044281 GO:0044283 GO:0044550 GO:0046148 GO:0046217 GO:0046283 GO:0046351 GO:0046394 GO:0046483 GO:0048366 GO:0048367 GO:0048519 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051171 GO:0051239 GO:0051241 GO:0051252 GO:0052314 GO:0052315 GO:0052317 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0090693 GO:0099402 GO:0140110 GO:1900055 GO:1900056 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607 GO:1903506 GO:1905622 GO:2000024 GO:2000026 GO:2000112 GO:2001141
PFAM: PF02365.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0212460	XP_003596151.2	77.288	295	49	2	297	573	18	312	1.85e-159	468
Msa0212460	XP_003596151.2	87.460	311	24	3	1	296	1	311	0.0	559

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0212460	sp\|Q9SK55\|NAC42_ARATH	50.883	283	106	10	16	289	12	270	5.20e-77	248
Msa0212460	sp\|Q9SK55\|NAC42_ARATH	65.934	182	42	5	289	465	6	172	4.73e-74	240

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0212460	G7IGZ8	77.288	295	49	2	297	573	18	312	8.83e-160	468
Msa0212460	G7IGZ8	87.460	311	24	3	1	296	1	311	0.0	559

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TFs/TRs:

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Gene ID	Type	Classification
Msa0212460	TF	NAC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0212460	MtrunA17_Chr2g0311651	87.460	311	24	3	1	296	1	311	0.0	559
Msa0212460	MtrunA17_Chr2g0311651	77.288	295	49	2	297	573	18	312	1.70e-163	468
Msa0212460	MtrunA17_Chr2g0311661	85.512	283	29	2	296	573	16	291	2.60e-178	504
Msa0212460	MtrunA17_Chr2g0311661	78.146	302	48	4	1	296	1	290	2.76e-169	481
Msa0212460	MtrunA17_Chr5g0442771	48.852	305	132	9	283	569	2	300	3.12e-86	270
Msa0212460	MtrunA17_Chr5g0442771	50.680	294	120	8	18	293	14	300	2.68e-85	268
Msa0212460	MtrunA17_Chr5g0417001	49.627	268	105	12	298	554	11	259	1.74e-66	221
Msa0212460	MtrunA17_Chr5g0417001	50.413	242	95	9	19	244	11	243	3.21e-66	220
Msa0212460	MtrunA17_Chr8g0361731	48.963	241	102	8	20	250	12	241	5.96e-65	217
Msa0212460	MtrunA17_Chr8g0361731	60.119	168	57	4	298	460	11	173	9.79e-64	214

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0212460	AT2G43000.1	50.883	283	106	10	16	289	12	270	5.29e-78	248
Msa0212460	AT2G43000.1	65.934	182	42	5	289	465	6	172	4.81e-75	240
Msa0212460	AT3G12910.1	46.535	303	126	12	10	291	9	296	7.16e-72	233
Msa0212460	AT3G12910.1	54.751	221	79	7	293	505	13	220	9.95e-71	230
Msa0212460	AT5G39820.1	52.830	212	75	7	298	488	17	224	8.07e-64	213
Msa0212460	AT5G39820.1	45.487	277	109	10	14	261	4	267	1.96e-61	206
Msa0212460	AT1G26870.1	45.324	278	125	6	20	292	14	269	1.93e-62	211
Msa0212460	AT1G26870.1	59.524	168	58	4	298	460	13	175	1.67e-59	204
Msa0212460	AT3G04070.2	49.133	173	76	6	16	182	5	171	3.48e-48	171
Msa0212460	AT3G04070.2	49.697	165	72	5	303	461	12	171	1.13e-46	167

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Find 85 sgRNAs with CRISPR-Local

Find 826 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACACTTGTTTACTACCG+TGG	0.605694	2_1:+54567244	Msa0212460:CDS
AAAACACTTGTTTACTATCG+TGG	0.541680	2_1:+54580009	Msa0212460:CDS
AAAGCAATATACTCAAATGG+AGG	0.648134	2_1:+54567193	Msa0212460:CDS
AAAGCAATATACTCAAATGG+AGG	0.648134	2_1:+54579958	Msa0212460:CDS
AAATGTAGCTGGAGCAGAGA+AGG	0.513323	2_1:+54579837	Msa0212460:CDS
AACATACTTGGAAGATCCCA+AGG	0.589388	2_1:-54578496	None:intergenic
AATTATACTATTTACAAACT+AGG	0.458098	2_1:-54581448	None:intergenic
ACCAAATATTTGGATTAAGC+AGG	0.451212	2_1:+54581354	Msa0212460:CDS
AGAGAAGATGGGTATGAACG+AGG	0.606606	2_1:+54566085	None:intergenic
AGATCAATTGAGCTCTTCAG+TGG	0.611925	2_1:+54568265	Msa0212460:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGATGAGATCAATAGCAAT+AGG	-	chr2_1:54566236-54566255	None:intergenic	30.0%
AAAATAAGAATAGGGTTTCC+AGG	+	chr2_1:54566426-54566445	Msa0212460:intron	30.0%
CATGCACAAATAGAAATAGA+TGG	+	chr2_1:54566483-54566502	Msa0212460:intron	30.0%
TTTGCACTATGTAGAAAAGT+GGG	-	chr2_1:54566967-54566986	None:intergenic	30.0%
ATTTGCACTATGTAGAAAAG+TGG	-	chr2_1:54566968-54566987	None:intergenic	30.0%
GTCAAAGAGGAAGAAAATAT+AGG	+	chr2_1:54567110-54567129	Msa0212460:CDS	30.0%
GACAAAGCAATATACTCAAA+TGG	+	chr2_1:54567190-54567209	Msa0212460:CDS	30.0%
AAAGCAATATACTCAAATGG+AGG	+	chr2_1:54567193-54567212	Msa0212460:CDS	30.0%
TAATAAGGCCAAAATTTGGT+AGG	-	chr2_1:54567410-54567429	None:intergenic	30.0%
ATATGCCAAAAAAATGAGAC+TGG	-	chr2_1:54567449-54567468	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	54566092	54581459	54566092	ID=Msa0212460;Name=Msa0212460
chr2_1	mRNA	54566092	54581459	54566092	ID=Msa0212460-mRNA-1;Parent=Msa0212460;Name=Msa0212460-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0\|0\|0\|1\|0.5\|0.22\|9\|0\|573
chr2_1	exon	54566092	54566296	54566092	ID=Msa0212460-mRNA-1:exon:15095;Parent=Msa0212460-mRNA-1
chr2_1	exon	54567068	54567265	54567068	ID=Msa0212460-mRNA-1:exon:15096;Parent=Msa0212460-mRNA-1
chr2_1	exon	54567281	54567387	54567281	ID=Msa0212460-mRNA-1:exon:15097;Parent=Msa0212460-mRNA-1
chr2_1	exon	54568017	54568239	54568017	ID=Msa0212460-mRNA-1:exon:15098;Parent=Msa0212460-mRNA-1
chr2_1	exon	54568267	54568419	54568267	ID=Msa0212460-mRNA-1:exon:15099;Parent=Msa0212460-mRNA-1
chr2_1	exon	54578356	54578511	54578356	ID=Msa0212460-mRNA-1:exon:15100;Parent=Msa0212460-mRNA-1
chr2_1	exon	54579833	54580030	54579833	ID=Msa0212460-mRNA-1:exon:15101;Parent=Msa0212460-mRNA-1
chr2_1	exon	54580046	54580152	54580046	ID=Msa0212460-mRNA-1:exon:15102;Parent=Msa0212460-mRNA-1
chr2_1	exon	54581085	54581459	54581085	ID=Msa0212460-mRNA-1:exon:15103;Parent=Msa0212460-mRNA-1
chr2_1	CDS	54566092	54566296	54566092	ID=Msa0212460-mRNA-1:cds;Parent=Msa0212460-mRNA-1
chr2_1	CDS	54567068	54567265	54567068	ID=Msa0212460-mRNA-1:cds;Parent=Msa0212460-mRNA-1
chr2_1	CDS	54567281	54567387	54567281	ID=Msa0212460-mRNA-1:cds;Parent=Msa0212460-mRNA-1
chr2_1	CDS	54568017	54568239	54568017	ID=Msa0212460-mRNA-1:cds;Parent=Msa0212460-mRNA-1
chr2_1	CDS	54568267	54568419	54568267	ID=Msa0212460-mRNA-1:cds;Parent=Msa0212460-mRNA-1
chr2_1	CDS	54578356	54578511	54578356	ID=Msa0212460-mRNA-1:cds;Parent=Msa0212460-mRNA-1
chr2_1	CDS	54579833	54580030	54579833	ID=Msa0212460-mRNA-1:cds;Parent=Msa0212460-mRNA-1
chr2_1	CDS	54580046	54580152	54580046	ID=Msa0212460-mRNA-1:cds;Parent=Msa0212460-mRNA-1
chr2_1	CDS	54581085	54581459	54581085	ID=Msa0212460-mRNA-1:cds;Parent=Msa0212460-mRNA-1

Gene Sequence

>Msa0212460

ATGGGTATGAACGAGGATTTCAACATGGATAATGAATATGTTGTAGATGATGAAAATGATGTTCCACTTCCTGGATTTCGTTTCCATCCTACAGATGAAGAACTTGTTGCTTTTTATCTTAGAAGAAAACTTGATAAGAAACCTATTGCTATTGATCTCATCAAACAAATTGATATCTACAAATATGATCCTTGGGATCTTCCAAAAGCCAATGTACCTGGAGCAGAAAAGGAAGGTTACTTTTTTTGTCAAAGAGGAAGAAAATATAGGAACAGCGTTAGACCAAATAGAGTCACTGGGTCTGGATTTTGGAAAGCAACTGGAATAGACAAAGCAATATACTCAAATGGAGGAGAAGGAAATGATTGTGTTGGACTCAAAAAAACACTTGTTTACTACCGTGGTACCAAAACTGATTGGATGATGCATGAGTTTCGTCTTCCATCAAACATTGATGCCAAAACAAACATTTCCTATGCTAAGAATGATGCTGATTATGCTCATGAAGCTGAAATTTGGACATTGTGTAGAATTTTCAAAAGAAATGTATCACAAAGGAAAAACATGGCAGATGTGAAACCCTTAGCTACCAAGCGTCAAACCATTCATGAAAAGAGTAGTAGAATGAATAGCAGTATGGAGTTCAACACAAATCAAGAAACATATATCAATTTTGGTACAAGCCAAGAACATCATCACAATGAGGACAAACCAGTCAACAATTATACAAGCAATCAATTGAGCTCTTCAGTGGAACATCAACCTCAACAAATTAATGTAACACAATCTTCAAATTTTTGGATTAAGCAAGCTGCTAATGAGTTATTATCATTTGATAATTGGGATGAGCTTGGATCAGTTGTGAACTTTGTTTCAGATTCACCTAATGATGATGATGTTCCACTTCCTGGATTTCGATTCCATCCCACTGATGAAGAACTTGTTACTTTTTATCTTCGAAGAAAACTTGATAAGAAACCTATTGCTATTGATCTTATCAAACAAATTGATATCTACAAATATGATCCTTGGGATCTTCCAAAAGCAAATGTAGCTGGAGCAGAGAAGGAATGTTACTTTTTTTGCCAAAGAGGAAGAAAATATAGGAACAGCGTTAGACCAAATAGAGTGACTGGCTCTGGATTTTGGAAAGCAACTGGAATAGACAAAGCAATATACTCAAATGGAGGAGAAGGAAATGATTGTGTTGGACTCAAAAAAACACTTGTTTACTATCGTGGCACCAAAACTGATTGGATGATGCATGAGTTTCGTCTTCCATCAACCATTGATGTCAAAACAAACATTTCCTATGCTAAGAATGATGCTGATTATGCTCATGAAGCTGAAATTTGGACATTGTGTAGAATTCTCAAAAGAAATGTATCACAAAGGAAACAAATACCGCAGATAAAACAATTAGCTAACAAGCATCAAGTCAGTCATAACACGAGCACCAGAATGAACAACAATATGGAGTTCAACATAAATCAACAAACATATATAAATTTTGGTGCAAGCCATGGACATCATCACATTCATGAGGACAAATCAATCAATAACTATACAAGCAGTGAAAAAAGCAATCAGTTTCATGCAAACAATGTAACACCATCACCAAATATTTGGATTAAGCAGGATGCTAATGAGTTGTTATCATTTGATAATTGGGATGAGCTTGGATCAGTTGTGAGGTTTGCTGTTGATTCACCTAGTTTGTAA

Protein sequence

>Msa0212460

MGMNEDFNMDNEYVVDDENDVPLPGFRFHPTDEELVAFYLRRKLDKKPIAIDLIKQIDIYKYDPWDLPKANVPGAEKEGYFFCQRGRKYRNSVRPNRVTGSGFWKATGIDKAIYSNGGEGNDCVGLKKTLVYYRGTKTDWMMHEFRLPSNIDAKTNISYAKNDADYAHEAEIWTLCRIFKRNVSQRKNMADVKPLATKRQTIHEKSSRMNSSMEFNTNQETYINFGTSQEHHHNEDKPVNNYTSNQLSSSVEHQPQQINVTQSSNFWIKQAANELLSFDNWDELGSVVNFVSDSPNDDDVPLPGFRFHPTDEELVTFYLRRKLDKKPIAIDLIKQIDIYKYDPWDLPKANVAGAEKECYFFCQRGRKYRNSVRPNRVTGSGFWKATGIDKAIYSNGGEGNDCVGLKKTLVYYRGTKTDWMMHEFRLPSTIDVKTNISYAKNDADYAHEAEIWTLCRILKRNVSQRKQIPQIKQLANKHQVSHNTSTRMNNNMEFNINQQTYINFGASHGHHHIHEDKSINNYTSSEKSNQFHANNVTPSPNIWIKQDANELLSFDNWDELGSVVRFAVDSPSL*