AlfalfaGEDB | Msa0215580

M. sativa cultivar zhongmu-4 / Msa0215580

KEGG: -
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010321 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0048580 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051239 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000026 GO:2000112 GO:2001141
PFAM: PF03110.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0215580	XP_013464662.2	93.220	177	12	0	1	177	36	212	3.92e-104	307

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0215580	sp\|Q38741\|SBP1_ANTMA	60.177	113	41	1	30	142	22	130	8.29e-39	132

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0215580	A0A072VA58	94.737	171	9	0	7	177	1	171	4.33e-101	297

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TFs/TRs:

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Gene ID	Type	Classification
Msa0215580	TF	SBP

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0215580	MtrunA17_Chr2g0317041	91.818	110	9	0	1	110	36	145	1.65e-56	175
Msa0215580	MtrunA17_Chr8g0360361	68.333	120	36	1	60	177	61	180	1.79e-51	163
Msa0215580	MtrunA17_Chr2g0317031	95.522	67	3	0	111	177	9	75	3.00e-42	136
Msa0215580	MtrunA17_Chr2g0282521	60.952	105	39	1	38	140	36	140	3.14e-38	128
Msa0215580	MtrunA17_Chr4g0046991	67.391	92	30	0	48	139	57	148	4.02e-38	128
Msa0215580	MtrunA17_Chr8g0334671	61.765	102	39	0	37	138	160	261	4.76e-36	127
Msa0215580	MtrunA17_Chr3g0122411	64.045	89	32	0	49	137	150	238	8.30e-36	129
Msa0215580	MtrunA17_Chr7g0258471	72.727	77	21	0	61	137	139	215	1.70e-34	125
Msa0215580	MtrunA17_Chr2g0302791	67.442	86	28	0	64	149	139	224	1.73e-34	128
Msa0215580	MtrunA17_Chr7g0270541	67.391	92	30	0	56	147	137	228	3.39e-34	127

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0215580	AT1G53160.3	63.551	107	36	2	50	156	42	145	5.41e-39	131
Msa0215580	AT1G53160.2	65.049	103	33	2	54	156	46	145	9.50e-39	130
Msa0215580	AT1G53160.1	65.049	103	33	2	54	156	46	145	9.50e-39	130
Msa0215580	AT3G15270.1	51.351	148	65	2	7	148	1	147	1.51e-38	130
Msa0215580	AT1G02065.1	61.321	106	37	2	50	151	171	276	9.16e-37	130
Msa0215580	AT2G33810.1	71.250	80	23	0	60	139	50	129	6.82e-36	122
Msa0215580	AT1G69170.1	56.731	104	45	0	34	137	94	197	2.99e-35	127
Msa0215580	AT3G60030.1	62.136	103	34	2	64	166	127	224	1.26e-34	129
Msa0215580	AT1G20980.1	60.784	102	38	1	54	155	112	211	8.57e-34	126
Msa0215580	AT2G42200.1	68.675	83	26	0	62	144	72	154	1.85e-33	122

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Find 36 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACATCAGTTGAGAAGGTT+TGG	0.391376	2_1:+60899544	Msa0215580:CDS
AACCACCACAACAGATGCTT+TGG	0.458273	2_1:-60899781	None:intergenic
AAGAAAGGTGTTTGTGTGAG+TGG	0.453403	2_1:+60899636	Msa0215580:CDS
AAGCAAGGAATACAATAGAG+GGG	0.716459	2_1:+60899517	Msa0215580:CDS
AATAGAAGTTGAAGAAGAGG+AGG	0.552525	2_1:+60899587	Msa0215580:CDS
AATGAACCTCACAAACCTTA+TGG	0.389353	2_1:-60899758	None:intergenic
ACTGCTGGAACTTGCACTGA+AGG	0.529046	2_1:+60902323	Msa0215580:CDS
ACTGGCCAGCAAAATCATGA+TGG	0.639807	2_1:+60902353	Msa0215580:CDS
AGAAAAGAGCCAAGTAGAGG+TGG	0.571945	2_1:+60899660	Msa0215580:CDS
AGAAAGGTGTTTGTGTGAGT+GGG	0.554222	2_1:+60899637	Msa0215580:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAAATAGAAGTTGAAGAAG+AGG	+	chr2_1:60899584-60899603	Msa0215580:CDS	30.0%
CAACTTTCTACTTGACAAAA+AGG	-	chr2_1:60899699-60899718	None:intergenic	30.0%
TTCATAAGTTTAGATGACAG+TGG	-	chr2_1:60899863-60899882	None:intergenic	30.0%
TCATCTAAACTTATGAAGCA+CGG	+	chr2_1:60899866-60899885	Msa0215580:intron	30.0%
AGTTTAGATTCTACGAAATG+CGG	-	chr2_1:60900045-60900064	None:intergenic	30.0%
AAAATTCTTGGATCCATTAG+TGG	-	chr2_1:60900187-60900206	None:intergenic	30.0%
TTTGGAAAACCTTTCTTTGT+TGG	-	chr2_1:60900701-60900720	None:intergenic	30.0%
AAAAATTCTCTCTCATCCAT+AGG	+	chr2_1:60901181-60901200	Msa0215580:intron	30.0%
ATTTGTTTGAAAAAGGTCCT+TGG	-	chr2_1:60901958-60901977	None:intergenic	30.0%
TTTCAAATAAGGTGAATGCA+TGG	-	chr2_1:60902198-60902217	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	60899513	60902442	60899513	ID=Msa0215580;Name=Msa0215580
chr2_1	mRNA	60899513	60902442	60899513	ID=Msa0215580-mRNA-1;Parent=Msa0215580;Name=Msa0215580-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0\|0\|0\|1\|1\|1\|2\|0\|177
chr2_1	exon	60899513	60899841	60899513	ID=Msa0215580-mRNA-1:exon:16770;Parent=Msa0215580-mRNA-1
chr2_1	exon	60902238	60902442	60902238	ID=Msa0215580-mRNA-1:exon:16771;Parent=Msa0215580-mRNA-1
chr2_1	CDS	60899513	60899841	60899513	ID=Msa0215580-mRNA-1:cds;Parent=Msa0215580-mRNA-1
chr2_1	CDS	60902238	60902442	60902238	ID=Msa0215580-mRNA-1:cds;Parent=Msa0215580-mRNA-1

Gene Sequence

>Msa0215580

ATGGAAGCAAGGAATACAATAGAGGGGAAGCAAACATCAGTTGAGAAGGTTTGGAAAGACATCTTATTTGAAGAAATAGAAGTTGAAGAAGAGGAGGAAGGTGGTGTTGAAGATGATGAGAAGAAGAAAGGTGTTTGTGTGAGTGGGAGAAAAGAGCCAAGTAGAGGTGGAAGATTTTCTCCACCTTTTTGTCAAGTAGAAAGTTGTGGAGCTGATTTAACTTTTGCAAAGAGATATCATCGTCGCCATAAGGTTTGTGAGGTTCATTCCAAAGCATCTGTTGTGGTGGTTGCAGGGATAAGGCAGAGGTTTTGCCAACAATGTAGCAGGTTTCATGAGCTTGCAGAGTTTGATGAATCGAAAAGAAGCTGTCGAAGACGCTTAGCGCGACACAATGAACGGCGTCGTAAAAGCACTGCTGGAACTTGCACTGAAGGAAGCGGTACTGGCCAGCAAAATCATGATGGTGATTGGAGACAAATTCATATGAACATAACAAGTAGTTCTGGTCACGATTTTCTGAATTTCAGATGA

Protein sequence

>Msa0215580

MEARNTIEGKQTSVEKVWKDILFEEIEVEEEEEGGVEDDEKKKGVCVSGRKEPSRGGRFSPPFCQVESCGADLTFAKRYHRRHKVCEVHSKASVVVVAGIRQRFCQQCSRFHELAEFDESKRSCRRRLARHNERRRKSTAGTCTEGSGTGQQNHDGDWRQIHMNITSSSGHDFLNFR*