AlfalfaGEDB | Msa0215720

M. sativa cultivar zhongmu-4 / Msa0215720

KEGG: -
GO: -
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0215720	KEH26464.1	57.798	109	23	2	425	516	77	179	1.75e-20	106
Msa0215720	KEH26464.1	69.002	471	93	8	1	424	2	466	0.0	633

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0215720	A0A072U9K2	57.798	109	23	2	425	516	77	179	8.36e-21	106
Msa0215720	A0A072U9K2	69.002	471	93	8	1	424	2	466	0.0	633

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TFs/TRs:

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Gene ID	Type	Classification
Msa0215720	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0215720	MtrunA17_Chr6g0476171	65.468	278	36	4	168	424	3	241	7.13e-120	352
Msa0215720	MtrunA17_Chr1g0163101	60.209	191	35	4	1	170	1	171	4.56e-67	214
Msa0215720	MtrunA17_Chr1g0163101	74.725	91	20	1	426	516	77	164	9.58e-36	131
Msa0215720	MtrunA17_Chr1g0163141	65.359	153	39	2	32	170	18	170	9.87e-57	187
Msa0215720	MtrunA17_Chr1g0163141	47.015	134	50	3	404	516	30	163	1.79e-25	103
Msa0215720	MtrunA17_Chr1g0163131	38.629	321	130	9	5	278	6	306	2.17e-56	191
Msa0215720	MtrunA17_Chr1g0163131	72.826	92	23	1	425	516	77	166	3.38e-33	128
Msa0215720	MtrunA17_Chr1g0163131	45.455	77	40	1	352	428	232	306	9.01e-14	72.4
Msa0215720	MtrunA17_Chr6g0476181	62.252	151	40	2	32	170	9	154	2.45e-55	182
Msa0215720	MtrunA17_Chr6g0476181	57.798	109	23	2	425	516	45	147	3.03e-26	104

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 86 sgRNAs with CRISPR-Local

Find 407 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGACATAATATATTTAG+TGG	0.334344	2_1:+61108728	Msa0215720:CDS
AAAATGAATTGGGTACAACG+AGG	0.756191	2_1:+61106734	Msa0215720:CDS
AACAATTAGAAGTGTTGATG+AGG	0.583313	2_1:+61107941	Msa0215720:CDS
AACAGCTGCCTTAAAACACA+AGG	0.666453	2_1:+61108437	Msa0215720:CDS
AAGGAAATTCGTAGAGACAT+TGG	0.511665	2_1:+61106660	Msa0215720:intron
AAGGAACATGACTTAAAAGT+TGG	0.442738	2_1:+61108044	Msa0215720:CDS
AATAGAGACAGAGGAGGCCG+CGG	0.610798	2_1:+61107351	Msa0215720:intron
AATCTGACCCTCCTTTGTAT+TGG	0.385935	2_1:-61106826	None:intergenic
AATTCGGTGGAGTTGAAAGT+AGG	0.502201	2_1:+61108752	Msa0215720:CDS
ACATATTCCATATCTTCTTC+GGG	0.475083	2_1:-61108389	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAATGAATGAAAACGGAAA+AGG	-	chr2_1:61105825-61105844	None:intergenic	30.0%
CATATGCTTGACTTAAAATG+TGG	+	chr2_1:61106475-61106494	Msa0215720:intron	30.0%
GTCTGAGTTCAAAATGAATT+GGG	+	chr2_1:61106724-61106743	Msa0215720:CDS	30.0%
CAATGATTATGATTGTGTTG+AGG	+	chr2_1:61106952-61106971	Msa0215720:CDS	30.0%
GATAATTTGAGAGTGAAACT+AGG	-	chr2_1:61107453-61107472	None:intergenic	30.0%
ATAGGAAACATACCAATCTA+TGG	-	chr2_1:61107494-61107513	None:intergenic	30.0%
GGTACAAAAATCACTATCAT+GGG	-	chr2_1:61107527-61107546	None:intergenic	30.0%
AGGTACAAAAATCACTATCA+TGG	-	chr2_1:61107528-61107547	None:intergenic	30.0%
TTAACAAGAGAAACATTTGG+GGG	-	chr2_1:61107611-61107630	None:intergenic	30.0%
AGCTAACAATAGAGACATTT+AGG	+	chr2_1:61107747-61107766	Msa0215720:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	61105704	61113786	61105704	ID=Msa0215720;Name=Msa0215720
chr2_1	mRNA	61105704	61113786	61105704	ID=Msa0215720-mRNA-1;Parent=Msa0215720;Name=Msa0215720-mRNA-1;_AED=0.14;_eAED=0.14;_QI=0\|0\|0\|1\|0.11\|0.2\|10\|0\|516
chr2_1	exon	61105704	61105796	61105704	ID=Msa0215720-mRNA-1:exon:16849;Parent=Msa0215720-mRNA-1
chr2_1	exon	61106674	61106840	61106674	ID=Msa0215720-mRNA-1:exon:16850;Parent=Msa0215720-mRNA-1
chr2_1	exon	61106877	61107125	61106877	ID=Msa0215720-mRNA-1:exon:16851;Parent=Msa0215720-mRNA-1
chr2_1	exon	61107356	61107482	61107356	ID=Msa0215720-mRNA-1:exon:16852;Parent=Msa0215720-mRNA-1
chr2_1	exon	61107885	61107977	61107885	ID=Msa0215720-mRNA-1:exon:16853;Parent=Msa0215720-mRNA-1
chr2_1	exon	61108023	61108164	61108023	ID=Msa0215720-mRNA-1:exon:16854;Parent=Msa0215720-mRNA-1
chr2_1	exon	61108354	61108467	61108354	ID=Msa0215720-mRNA-1:exon:16855;Parent=Msa0215720-mRNA-1
chr2_1	exon	61108559	61108617	61108559	ID=Msa0215720-mRNA-1:exon:16856;Parent=Msa0215720-mRNA-1
chr2_1	exon	61108701	61108928	61108701	ID=Msa0215720-mRNA-1:exon:16857;Parent=Msa0215720-mRNA-1
chr2_1	exon	61113508	61113786	61113508	ID=Msa0215720-mRNA-1:exon:16858;Parent=Msa0215720-mRNA-1
chr2_1	CDS	61105704	61105796	61105704	ID=Msa0215720-mRNA-1:cds;Parent=Msa0215720-mRNA-1
chr2_1	CDS	61106674	61106840	61106674	ID=Msa0215720-mRNA-1:cds;Parent=Msa0215720-mRNA-1
chr2_1	CDS	61106877	61107125	61106877	ID=Msa0215720-mRNA-1:cds;Parent=Msa0215720-mRNA-1
chr2_1	CDS	61107356	61107482	61107356	ID=Msa0215720-mRNA-1:cds;Parent=Msa0215720-mRNA-1
chr2_1	CDS	61107885	61107977	61107885	ID=Msa0215720-mRNA-1:cds;Parent=Msa0215720-mRNA-1
chr2_1	CDS	61108023	61108164	61108023	ID=Msa0215720-mRNA-1:cds;Parent=Msa0215720-mRNA-1
chr2_1	CDS	61108354	61108467	61108354	ID=Msa0215720-mRNA-1:cds;Parent=Msa0215720-mRNA-1
chr2_1	CDS	61108559	61108617	61108559	ID=Msa0215720-mRNA-1:cds;Parent=Msa0215720-mRNA-1
chr2_1	CDS	61108701	61108928	61108701	ID=Msa0215720-mRNA-1:cds;Parent=Msa0215720-mRNA-1
chr2_1	CDS	61113508	61113786	61113508	ID=Msa0215720-mRNA-1:cds;Parent=Msa0215720-mRNA-1

Gene Sequence

>Msa0215720

ATGTCAAGTGTTCACTATGGACGTGGGAGTGAAGCTAAGGGAATGCATTTTTACCAAACCATTCAACTTCGCCAGCTTCAAGACCAAGAACTAAGACATTGGAAAGATGTATCAAATCCAACATCCCTTAGACTTCCAAATGGGTCTGAGTTCAAAATGAATTGGGTACAACGAGGTGATGATGTTTGGCTTCAGAATTGGAAGAGGTTTGCGCGCTCTCTCAGATGTGGAGATCTTGTAGTTTTCCAATACAAAGGAGGTTTGGAAATTGACTATTCATGTATGAGATGCAATGATGAACAAGAAGCTAAGGATAGTGATGATGATGACAATGACAATGATTATGATTGTGTTGAGGTTCTGAATGACGGTGAAAATACCAGACAACCCCTCAAGAAAAAGAAAATCAACTCAAATGGCATTGCTACTGTTGCTGCTACACCTCAAGGGACAAACATTGACAAGAGAAAAATTAATATCAATGCAACCCTGCAAAAAATTTCAGGCAAAGACAGAGGAGGCCGCGGTAAGAAAGCTAAGAAATGCACAAAAACTAAACATGGGAATGAGAGAAGCTGCACAAATTATCATCCCAATGAGAATCCTAGTTTCACTCTCAAATTATCCCGATCTCATAGGCTACCTACATGGTTTTCAAACGAATACATGAATGAGTTATTGCAAGGGAAGGCAACAATTAGAAGTGTTGATGAGGACAGGGCACGGTACGTTGGTTGGAAGTCATTTTCAAAGGAACATGACTTAAAAGTTGGTGATGCATGTAAATTTGAGATGACTCAACGCGAACCACTTTCATTCACAATTACCATTATCCCAGCCACAAAGGAACCATGCCCAAAGCAGTTTCAAGAAAACCAACAAGTGCAAATAAAATCAGAGACAATGCCCGAAGAAGATATGGAATATGTGGGCAATTTTGGAGAACAAATTTCAAACAGCTGCCTTAAAACACAAGGTCTCAGGAATTCTGTAAGTCCGAATTTACATGACAAATTCAAGGTTTTTGTGACATCATTAGACAAGTTGGTTGTACTACCAAGTGAGCTGTTAAAAAGACATAATATATTTAGTGGAAATTCGGTGGAGTTGAAAGTAGGGGAAGGAACATGGTTTGTAGAGGTGAGTTATAATGAACATCTCGACTATGGATGGTTCACTAAAGGCTGGCAAGAGTTTCTGAGAGATTGCAAAGTAAAGACTGGAGACACTTGCTTGTTTGAGATGATTGATGTGCAAAATCATGTGTTCAAGAAAAGGTTTGCATGGTCACTTAGATGTAGAGATCTTTTAGTTTTTCAATATAAAGGAGGGTCATATTTTCATGTCGTTATATTAGATGAAAGTAAATTAGAAATTGACTATTCAAGTTTGAGATACAATGATGACCAAAACTCTAACAAATATCGTAAACAAGAAGAAGAGAGTGATGATGATGATTGTCCTGAGATTTTGAATGATATTACTACTACTTCTACTACACCTCAAAAGACAAATATTGACAAGAGAAAAATTAATATGAATGCAACCTAA

Protein sequence

>Msa0215720

MSSVHYGRGSEAKGMHFYQTIQLRQLQDQELRHWKDVSNPTSLRLPNGSEFKMNWVQRGDDVWLQNWKRFARSLRCGDLVVFQYKGGLEIDYSCMRCNDEQEAKDSDDDDNDNDYDCVEVLNDGENTRQPLKKKKINSNGIATVAATPQGTNIDKRKININATLQKISGKDRGGRGKKAKKCTKTKHGNERSCTNYHPNENPSFTLKLSRSHRLPTWFSNEYMNELLQGKATIRSVDEDRARYVGWKSFSKEHDLKVGDACKFEMTQREPLSFTITIIPATKEPCPKQFQENQQVQIKSETMPEEDMEYVGNFGEQISNSCLKTQGLRNSVSPNLHDKFKVFVTSLDKLVVLPSELLKRHNIFSGNSVELKVGEGTWFVEVSYNEHLDYGWFTKGWQEFLRDCKVKTGDTCLFEMIDVQNHVFKKRFAWSLRCRDLLVFQYKGGSYFHVVILDESKLEIDYSSLRYNDDQNSNKYRKQEEESDDDDCPEILNDITTTSTTPQKTNIDKRKINMNAT*