AlfalfaGEDB | Msa0216630

M. sativa cultivar zhongmu-4 / Msa0216630

KEGG: K04733
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019899 GO:0031625 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044389 GO:0044464 GO:0046777 GO:0071704 GO:0071944 GO:0140096 GO:1901564
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0216630	XP_013464762.1	78.107	676	50	4	1	598	132	787	0.0	1030

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0216630	sp\|Q8RWZ5\|SD25_ARATH	53.323	647	208	11	25	598	196	821	0.0	640

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0216630	A0A072VAH2	78.107	676	50	4	1	598	132	787	0.0	1030

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0216630	MtrunA17_Chr2g0318171	78.107	676	50	4	1	598	132	787	0.0	1030
Msa0216630	MtrunA17_Chr2g0314611	70.778	681	93	7	1	598	132	789	0.0	904
Msa0216630	MtrunA17_Chr3g0137741	69.811	636	108	8	25	598	204	817	0.0	855
Msa0216630	MtrunA17_Chr1g0149381	55.105	715	178	15	1	598	137	825	0.0	692
Msa0216630	MtrunA17_Chr2g0333091	51.789	587	230	9	41	598	252	814	0.0	566
Msa0216630	MtrunA17_Chr3g0114851	34.615	676	311	17	6	576	177	826	1.19e-106	341
Msa0216630	MtrunA17_Chr1g0202331	40.530	528	237	17	86	565	308	806	4.09e-106	339
Msa0216630	MtrunA17_Chr6g0467281	50.427	351	148	8	246	591	461	790	9.40e-105	335
Msa0216630	MtrunA17_Chr6g0467301	38.765	583	282	19	50	591	247	795	1.83e-104	334
Msa0216630	MtrunA17_Chr4g0023071	43.294	425	205	10	153	573	382	774	3.42e-101	325

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0216630	AT4G32300.1	53.323	647	208	11	25	598	196	821	0.0	640
Msa0216630	AT1G34300.1	38.926	596	294	20	42	597	229	794	1.84e-106	340
Msa0216630	AT5G35370.1	36.511	556	267	16	77	572	283	812	1.68e-105	338
Msa0216630	AT5G24080.3	38.129	556	266	16	86	593	182	707	4.88e-105	333
Msa0216630	AT5G24080.1	38.129	556	266	16	86	593	182	707	4.88e-105	333
Msa0216630	AT2G19130.1	38.269	520	269	11	77	573	286	776	6.38e-104	333
Msa0216630	AT5G24080.2	39.238	525	244	15	86	565	309	803	9.56e-104	333
Msa0216630	AT4G00340.1	37.670	515	268	15	77	565	274	761	4.77e-90	296
Msa0216630	AT5G20050.1	42.480	379	177	6	218	575	42	400	3.00e-87	278
Msa0216630	AT4G00340.2	37.109	512	254	15	77	565	274	740	1.14e-86	286

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Find 133 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTGATGTATACAGCTA+TGG	0.477077	2_1:-62724972	Msa0216630:CDS
AAAAGTTGTTCAAATGTTAG+AGG	0.428209	2_1:-62724718	Msa0216630:CDS
AAAGTGAATTTCTTCTGGAT+TGG	0.364489	2_1:-62725447	Msa0216630:CDS
AAAGTGATGTATACAGCTAT+GGG	0.550881	2_1:-62724971	Msa0216630:CDS
AAAGTTGTTCAAATGTTAGA+GGG	0.571298	2_1:-62724717	Msa0216630:CDS
AAGACATGATGTTTGACAAC+TGG	0.410808	2_1:+62726077	None:intergenic
AAGCATTCATCACCTTAATT+TGG	0.204204	2_1:-62725569	Msa0216630:CDS
AAGCTGGAAGGTATTGGTCA+AGG	0.586413	2_1:-62725628	Msa0216630:CDS
AAGCTTGGTCAAGGAGGTTT+TGG	0.272727	2_1:-62725700	Msa0216630:CDS
AAGTGAATTTCTTCTGGATT+GGG	0.365810	2_1:-62725446	Msa0216630:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACGACAATAATACGGTAATT+TGG	-	chr2_1:62724657-62724676	Msa0216630:CDS	30.0%
ACCCCACTTATTAAAATTTC+TGG	-	chr2_1:62724753-62724772	Msa0216630:CDS	30.0%
AAACACAATTAGCAAAGCAA+TGG	-	chr2_1:62724936-62724955	Msa0216630:CDS	30.0%
ATTGCAAGACACTGAAAATT+TGG	-	chr2_1:62724960-62724979	Msa0216630:CDS	30.0%
TTTATTTGAACTAGGTTCAC+TGG	+	chr2_1:62725091-62725110	None:intergenic	30.0%
TAGGCCAATTTATTTGAACT+AGG	+	chr2_1:62725099-62725118	None:intergenic	30.0%
AGATTTGATCTCAAGAACAT+AGG	+	chr2_1:62725118-62725137	None:intergenic	30.0%
TATGTTCTTGAGATCAAATC+TGG	-	chr2_1:62725117-62725136	Msa0216630:CDS	30.0%
TTTCAAAACTCCAGACTTAT+TGG	-	chr2_1:62725161-62725180	Msa0216630:CDS	30.0%
TCAGAATGATAACCGTAAAA+CGG	-	chr2_1:62725189-62725208	Msa0216630:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	62724614	62727128	62724614	ID=Msa0216630;Name=Msa0216630
chr2_1	mRNA	62724614	62727128	62724614	ID=Msa0216630-mRNA-1;Parent=Msa0216630;Name=Msa0216630-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0\|0\|0\|0.6\|1\|1\|5\|0\|598
chr2_1	exon	62727072	62727128	62727072	ID=Msa0216630-mRNA-1:exon:17386;Parent=Msa0216630-mRNA-1
chr2_1	exon	62726492	62726585	62726492	ID=Msa0216630-mRNA-1:exon:17385;Parent=Msa0216630-mRNA-1
chr2_1	exon	62725439	62726399	62725439	ID=Msa0216630-mRNA-1:exon:17384;Parent=Msa0216630-mRNA-1
chr2_1	exon	62725313	62725388	62725313	ID=Msa0216630-mRNA-1:exon:17383;Parent=Msa0216630-mRNA-1
chr2_1	exon	62724614	62725222	62724614	ID=Msa0216630-mRNA-1:exon:17382;Parent=Msa0216630-mRNA-1
chr2_1	CDS	62727072	62727128	62727072	ID=Msa0216630-mRNA-1:cds;Parent=Msa0216630-mRNA-1
chr2_1	CDS	62726492	62726585	62726492	ID=Msa0216630-mRNA-1:cds;Parent=Msa0216630-mRNA-1
chr2_1	CDS	62725439	62726399	62725439	ID=Msa0216630-mRNA-1:cds;Parent=Msa0216630-mRNA-1
chr2_1	CDS	62725313	62725388	62725313	ID=Msa0216630-mRNA-1:cds;Parent=Msa0216630-mRNA-1
chr2_1	CDS	62724614	62725222	62724614	ID=Msa0216630-mRNA-1:cds;Parent=Msa0216630-mRNA-1

Gene Sequence

>Msa0216630

ATGGAATTGCAAGGCACTGGAAATTTGGTTTTGCTTGGTAACGACGACAATAATACGCTGGTTTCAAAACTCCAGACTTATTGGACTATTCAGAATGATAACCGTAAAACGGTTAACATGAATGGCGATGTTGTGGCTTTTGCAAATCTCATTTTAGGCAAAGACGGTGTCATTACTTTCTTGAATCTCAACGGTACTAGATCAAACGGTGATGCTTCACTAAGAATACCAAGAGATCCTTGTGGTACACCAGAACCTTGTGATCCCTACGGAATATGCACAAACAACCGTATGTGTAGCTGTCCTCTTGTTCTTCTTCCTAGTTGTAAACCGGGTTTTGCCTCTCCTTGCGATGATAAATCAAAAATATCTGTTGAATTTCTGAAAGTTGATGACGGACTTGGTTATTTTGCGCTTGATTTTCTTCGTCCCTATTCGAATACTGATTTATCCAGTTGTCAAACATCATGTCTTGGAAATTGCTCTTGTCTTGCAATGTTCTTTCATAGAAGTTCAGGGAACTGTTTCTTGTTGGACAGTGTTGGAAGTTTTCAGAAATCTGATGATGCTAATGCTTCTGGTTATGTTTCTTACATTAAGGTTGCTCGTGATGGAGGTCAAAGAAGCAATAAGCACATCATAGTTGTTGTGATCGTTGTCATAACAACTTTTCTTGTTCTGCTCTTTATGGGGGTTCTATACTACAAGAAAAAGAAAAAGTTACCTCGAGAGAATTCAGAAGAAGAAAATTTCTTGGAGAGTTTAACCGGCATGCCAATCCGTTTCCGCTATAAAGATCTTGAAGTTGCAACTAATAACTTCTCTGTGAAGCTTGGTCAAGGAGGTTTTGGATCAGTTTATAAAGGGCTTCTGCCTGATGGGACTGAATTAGCTGTGAAGAAGCTGGAAGGTATTGGTCAAGGGAAGAAAGAGTTCAGAGCTGAAGTTAGCATCATCGGAAGCATTCATCACCTTAATTTGGTAAGGCTAAAAGGATTTTCTGCAGATGGAACTCATAGGCTTCTAGTTTACGAGTACATGGCTAATAACTCATTGGACAAATGGATATTCAAGAAGAAAAAAAGTGAATTTCTTCTGGATTGGGATACAAGAGTAAAAGGGCAACCCGGTGCACTAAAGCTCCCGCATATGCGGGGTCCAGGAAGGGGTCCCACCATTTGGTGTGTTTTGCGCCAAGGTTACCCCTCTTATCTGCATCAAGAGTGTGACTCAAAAATTGTCCACTGTGATATCAAGCCTGAAAATGTACTTCTAGATGATCATTTCATTGCCAAAGTTTTGGATTTTGGTTTAGCTAAGCTTATGAATAGAGAACAAAGCCATGTTTTTACAACATTAAGAGGAACTCGTGGCTATCTTGCGCCTGAGTGGATAACAAACTCTGCTATATCAGAGAAAAGTGATGTATACAGCTATGGGATGGTGTTGCTAGAGATAATTGGGGGGAGGAAGAATTATGATCCTACCGAGACGTCGGAGAAATTCAATTTCCCTCGTTTTGCGTTTAAGATGATGGAAGAAGGGAAAGTGACAGATATACTTGATTCAGAGTTGAAAATAGATGATGAGAATGATGATAGGGTTCATTGTGCTATAAGTACTGCATTGTGGTGTATACAGGAAGACATGTCAATGAGACCTTCCATGACAAAAGTTGTTCAAATGTTAGAGGGTCTTTGCACTGTTCCTAAACCACCAAAATCCTCCAATGAAGGAAACACCTCCTCTTCTTCTGATGCTTATCTATCAGCAGTTCGTCTTTCAGGCCCGAGATAG

Protein sequence

>Msa0216630

MELQGTGNLVLLGNDDNNTLVSKLQTYWTIQNDNRKTVNMNGDVVAFANLILGKDGVITFLNLNGTRSNGDASLRIPRDPCGTPEPCDPYGICTNNRMCSCPLVLLPSCKPGFASPCDDKSKISVEFLKVDDGLGYFALDFLRPYSNTDLSSCQTSCLGNCSCLAMFFHRSSGNCFLLDSVGSFQKSDDANASGYVSYIKVARDGGQRSNKHIIVVVIVVITTFLVLLFMGVLYYKKKKKLPRENSEEENFLESLTGMPIRFRYKDLEVATNNFSVKLGQGGFGSVYKGLLPDGTELAVKKLEGIGQGKKEFRAEVSIIGSIHHLNLVRLKGFSADGTHRLLVYEYMANNSLDKWIFKKKKSEFLLDWDTRVKGQPGALKLPHMRGPGRGPTIWCVLRQGYPSYLHQECDSKIVHCDIKPENVLLDDHFIAKVLDFGLAKLMNREQSHVFTTLRGTRGYLAPEWITNSAISEKSDVYSYGMVLLEIIGGRKNYDPTETSEKFNFPRFAFKMMEEGKVTDILDSELKIDDENDDRVHCAISTALWCIQEDMSMRPSMTKVVQMLEGLCTVPKPPKSSNEGNTSSSSDAYLSAVRLSGPR*