AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0218070

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0218070 MtrunA17_Chr2g0320011 97.297 222 4 1 1 222 1 220 3.93e-158 444
Msa0218070 MtrunA17_Chr2g0284091 88.372 172 20 0 51 222 1 172 3.60e-113 328
Msa0218070 MtrunA17_Chr1g0193081 77.907 172 38 0 51 222 36 207 4.35e-99 293
Msa0218070 MtrunA17_Chr7g0270371 77.457 173 38 1 51 222 35 207 4.46e-97 288
Msa0218070 MtrunA17_Chr1g0194201 74.286 175 45 0 48 222 33 207 5.32e-96 286
Msa0218070 MtrunA17_Chr8g0350311 69.540 174 53 0 49 222 98 271 6.32e-88 267
Msa0218070 MtrunA17_Chr7g0246591 70.414 169 50 0 54 222 105 273 1.57e-87 266
Msa0218070 MtrunA17_Chr2g0277011 67.262 168 55 0 55 222 55 222 2.30e-83 254
Msa0218070 MtrunA17_Chr4g0065851 35.252 139 79 3 90 222 25 158 7.28e-22 93.2
Msa0218070 MtrunA17_Chr2g0287271 32.121 165 98 4 64 222 2 158 1.24e-20 89.7
Msa0218070 MtrunA17_Chr3g0096631 33.094 139 82 3 90 222 25 158 7.76e-20 87.4
Msa0218070 MtrunA17_Chr6g0482681 33.333 147 84 4 82 222 84 222 2.82e-19 85.9
Msa0218070 MtrunA17_Chr5g0443351 33.813 139 81 3 90 222 22 155 5.45e-19 84.7
Msa0218070 MtrunA17_Chr5g0409421 36.567 134 67 5 96 222 10 132 1.18e-18 83.6
Msa0218070 MtrunA17_Chr8g0392811 30.935 139 85 3 90 222 117 250 1.23e-18 84.0
Msa0218070 MtrunA17_Chr8g0369451 37.313 134 66 5 96 222 10 132 1.73e-17 80.5
Msa0218070 MtrunA17_Chr7g0218071 33.793 145 83 4 84 222 114 251 1.30e-16 78.2
Msa0218070 MtrunA17_Chr8g0348481 36.296 135 75 3 94 222 41 170 1.38e-16 77.4
Msa0218070 MtrunA17_Chr8g0385971 34.228 149 85 4 80 222 80 221 1.60e-16 77.8
Msa0218070 MtrunA17_Chr8g0359091 30.612 147 88 4 82 222 21 159 1.86e-16 77.4
Msa0218070 MtrunA17_Chr8g0387401 37.037 135 74 4 94 222 56 185 2.06e-16 77.0
Msa0218070 MtrunA17_Chr7g0248461 36.296 135 75 3 94 222 42 171 2.77e-16 76.6
Msa0218070 MtrunA17_Chr3g0130781 32.117 137 79 5 96 222 24 156 7.53e-16 75.5
Msa0218070 MtrunA17_Chr2g0314321 30.935 139 85 3 90 222 25 158 1.28e-15 75.1
Msa0218070 MtrunA17_Chr3g0099951 34.965 143 72 5 90 222 14 145 1.73e-15 74.3
Msa0218070 MtrunA17_Chr2g0317501 35.862 145 80 4 84 222 128 265 2.92e-15 74.3
Msa0218070 MtrunA17_Chr5g0397261 33.333 135 79 3 94 222 45 174 3.57e-15 73.6
Msa0218070 MtrunA17_Chr4g0030151 32.331 133 79 3 96 222 45 172 3.57e-15 73.6
Msa0218070 MtrunA17_Chr1g0201571 32.639 144 84 4 85 222 121 257 3.92e-15 73.6
Msa0218070 MtrunA17_Chr5g0396361 34.899 149 84 5 80 222 130 271 5.74e-15 73.2
Msa0218070 MtrunA17_Chr4g0061141 34.667 150 75 5 84 222 99 236 7.51e-15 72.8
Msa0218070 MtrunA17_Chr3g0131861 33.951 162 88 6 67 222 80 228 8.23e-15 72.8
Msa0218070 MtrunA17_Chr8g0383451 34.328 134 75 4 95 222 3 129 1.64e-14 71.6
Msa0218070 MtrunA17_Chr6g0453711 30.872 149 82 5 84 222 104 241 1.64e-14 72.0
Msa0218070 MtrunA17_Chr4g0030231 34.586 133 76 3 96 222 46 173 2.69e-14 70.9
Msa0218070 MtrunA17_Chr6g0483151 30.405 148 86 5 83 222 5 143 3.68e-14 70.5
Msa0218070 MtrunA17_Chr2g0286421 33.103 145 84 4 84 222 96 233 6.16e-14 70.1
Msa0218070 MtrunA17_Chr4g0051261 32.593 135 73 5 96 222 10 134 9.45e-14 68.9
Msa0218070 MtrunA17_Chr4g0066691 33.103 145 84 4 84 222 98 235 1.19e-13 69.3
Msa0218070 MtrunA17_Chr7g0272841 28.767 146 81 5 90 222 68 203 3.24e-13 68.2
Msa0218070 MtrunA17_Chr2g0304111 33.117 154 83 7 78 222 11 153 6.10e-13 67.0
Msa0218070 MtrunA17_Chr8g0379091 33.577 137 72 5 94 222 30 155 1.44e-12 66.2
Msa0218070 MtrunA17_Chr5g0441011 34.694 147 76 6 85 222 8 143 1.80e-12 65.9
Msa0218070 MtrunA17_Chr4g0013131 30.496 141 79 5 90 222 12 141 5.48e-12 64.3
Msa0218070 MtrunA17_Chr2g0284031 29.448 163 91 6 73 222 9 160 5.52e-12 64.3
Msa0218070 MtrunA17_Chr8g0339221 30.435 138 82 6 90 222 4 132 6.16e-12 64.3
Msa0218070 MtrunA17_Chr2g0293491 32.090 134 75 5 96 222 10 134 8.51e-12 63.2
Msa0218070 MtrunA17_Chr1g0150321 35.915 142 70 6 90 222 13 142 1.05e-11 63.5
Msa0218070 MtrunA17_Chr3g0093571 29.861 144 80 4 96 222 31 170 2.09e-11 62.8
Msa0218070 MtrunA17_Chr4g0063881 32.394 142 76 5 90 222 11 141 2.14e-11 62.8
Msa0218070 MtrunA17_Chr4g0001331 32.867 143 73 8 90 222 20 149 3.28e-11 62.0
Msa0218070 MtrunA17_Chr3g0107011 30.986 142 77 6 90 222 14 143 3.37e-11 62.0
Msa0218070 MtrunA17_Chr8g0358921 28.188 149 92 6 82 222 41 182 3.47e-11 61.6
Msa0218070 MtrunA17_Chr5g0432471 30.986 142 77 6 90 222 12 141 4.68e-11 61.6
Msa0218070 MtrunA17_Chr1g0205421 32.394 142 75 5 90 222 12 141 5.18e-11 61.6
Msa0218070 MtrunA17_Chr3g0114511 31.469 143 73 6 90 222 32 159 6.27e-11 61.2
Msa0218070 MtrunA17_Chr1g0149711 33.333 144 84 6 85 222 84 221 7.61e-11 60.8
Msa0218070 MtrunA17_Chr3g0093391 29.861 144 80 4 96 222 29 168 7.67e-11 61.2
Msa0218070 MtrunA17_Chr8g0346081 30.556 144 84 4 86 222 13 147 7.83e-11 60.8
Msa0218070 MtrunA17_Chr5g0428031 30.282 142 79 5 90 222 9 139 9.38e-11 60.8
Msa0218070 MtrunA17_Chr7g0273601 29.940 167 88 6 67 222 90 238 9.39e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0218070 AT1G06390.2 75.676 222 34 2 1 222 1 202 1.11e-117 341
Msa0218070 AT1G06390.1 75.676 222 34 2 1 222 1 202 1.11e-117 341
Msa0218070 AT2G30980.1 74.324 222 39 3 1 222 1 204 8.29e-114 332
Msa0218070 AT4G18710.1 86.628 172 23 0 51 222 1 172 2.66e-109 319
Msa0218070 AT4G18710.2 87.349 166 21 0 57 222 1 166 4.02e-108 312
Msa0218070 AT5G26751.1 79.070 172 36 0 51 222 30 201 2.96e-100 296
Msa0218070 AT5G14640.1 79.651 172 35 0 51 222 35 206 1.96e-99 295
Msa0218070 AT5G14640.2 79.651 172 35 0 51 222 35 206 1.96e-99 295
Msa0218070 AT3G05840.2 77.326 172 39 0 51 222 34 205 4.12e-99 294
Msa0218070 AT3G05840.1 77.326 172 39 0 51 222 34 205 4.12e-99 294
Msa0218070 AT4G00720.1 71.006 169 49 0 54 222 102 270 2.60e-88 268
Msa0218070 AT3G61160.6 64.740 173 61 0 50 222 69 241 1.56e-81 248
Msa0218070 AT3G61160.2 64.740 173 61 0 50 222 69 241 1.49e-80 248
Msa0218070 AT3G61160.3 64.327 171 61 0 52 222 64 234 1.46e-79 242
Msa0218070 AT3G61160.5 64.327 171 61 0 52 222 64 234 2.18e-79 243
Msa0218070 AT3G61160.1 64.327 171 61 0 52 222 64 234 1.43e-78 242
Msa0218070 AT3G61160.4 64.327 171 61 0 52 222 64 234 1.52e-78 242
Msa0218070 AT1G09840.3 63.793 174 61 1 51 222 42 215 5.09e-78 240
Msa0218070 AT1G09840.7 63.793 174 61 1 51 222 42 215 5.09e-78 240
Msa0218070 AT1G09840.10 63.793 174 61 1 51 222 42 215 5.09e-78 240
Msa0218070 AT1G09840.6 63.793 174 61 1 51 222 42 215 5.09e-78 240
Msa0218070 AT1G09840.4 63.793 174 61 1 51 222 42 215 5.09e-78 240
Msa0218070 AT1G09840.1 63.793 174 61 1 51 222 42 215 5.09e-78 240
Msa0218070 AT1G09840.9 63.793 174 61 1 51 222 42 215 5.09e-78 240
Msa0218070 AT1G09840.5 63.793 174 61 1 51 222 42 215 5.09e-78 240
Msa0218070 AT1G09840.8 63.793 174 61 1 51 222 42 215 5.09e-78 240
Msa0218070 AT1G09840.2 63.793 174 61 1 51 222 42 215 5.09e-78 240
Msa0218070 AT1G57870.1 63.584 173 61 1 52 222 42 214 5.76e-78 240
Msa0218070 AT1G57870.4 63.584 173 61 1 52 222 42 214 5.76e-78 240
Msa0218070 AT1G57870.2 63.584 173 61 1 52 222 42 214 5.76e-78 240
Msa0218070 AT1G57870.3 63.584 173 61 1 52 222 42 214 1.38e-77 240
Msa0218070 AT1G09840.11 64.773 88 31 0 135 222 1 88 1.36e-35 127
Msa0218070 AT5G19010.1 34.532 139 80 3 90 222 25 158 1.57e-20 89.7
Msa0218070 AT3G18040.5 31.410 156 93 4 73 222 4 151 1.36e-19 87.0
Msa0218070 AT3G18040.1 31.410 156 93 4 73 222 9 156 1.48e-19 87.0
Msa0218070 AT1G18150.3 34.014 147 83 4 82 222 99 237 1.67e-19 87.0
Msa0218070 AT1G18150.2 34.014 147 83 4 82 222 99 237 1.67e-19 87.0
Msa0218070 AT1G18150.1 34.014 147 83 4 82 222 99 237 1.67e-19 87.0
Msa0218070 AT3G18040.4 32.051 156 92 4 73 222 118 265 1.87e-19 86.7
Msa0218070 AT3G18040.3 32.051 156 92 4 73 222 118 265 2.65e-19 86.3
Msa0218070 AT2G42880.1 32.374 139 83 3 90 222 25 158 5.77e-19 85.5
Msa0218070 AT2G42880.2 32.374 139 83 3 90 222 25 158 6.62e-19 84.7
Msa0218070 AT1G73670.1 34.014 147 83 4 82 222 85 223 6.72e-19 85.1
Msa0218070 AT4G19110.2 36.364 132 70 4 96 222 10 132 2.84e-18 83.2
Msa0218070 AT4G19110.1 36.364 132 70 4 96 222 10 132 2.93e-18 83.2
Msa0218070 AT2G01450.3 33.094 139 82 3 90 222 16 149 3.86e-18 82.8
Msa0218070 AT2G01450.1 33.094 139 82 3 90 222 16 149 3.86e-18 82.8
Msa0218070 AT2G01450.2 33.094 139 82 3 90 222 16 149 3.86e-18 82.8
Msa0218070 AT2G01450.4 33.094 139 82 3 90 222 16 149 3.86e-18 82.8
Msa0218070 AT3G14720.1 31.655 139 84 3 90 222 25 158 5.72e-17 79.3
Msa0218070 AT4G01370.1 34.815 135 77 3 94 222 47 176 7.95e-17 78.6
Msa0218070 AT1G53510.1 30.935 139 85 3 90 222 25 158 1.82e-16 78.2
Msa0218070 AT3G14720.2 31.655 139 84 3 90 222 25 158 1.87e-16 77.8
Msa0218070 AT3G14720.3 31.655 139 84 3 90 222 25 158 1.87e-16 77.8
Msa0218070 AT3G59790.1 31.683 202 100 9 46 222 5 193 2.22e-16 77.4
Msa0218070 AT1G71530.4 30.841 214 115 8 15 222 92 278 5.69e-16 75.9
Msa0218070 AT1G71530.3 30.841 214 115 8 15 222 92 278 6.50e-16 76.3
Msa0218070 AT1G71530.2 31.401 207 109 8 22 222 99 278 7.43e-16 76.3
Msa0218070 AT1G71530.1 31.401 207 109 8 22 222 99 278 8.90e-16 75.9
Msa0218070 AT4G11330.1 33.333 135 79 3 94 222 47 176 2.37e-15 74.3
Msa0218070 AT1G33770.1 35.032 157 86 7 75 222 123 272 2.75e-15 74.7
Msa0218070 AT1G18670.2 34.483 145 82 5 84 222 125 262 8.61e-15 73.2
Msa0218070 AT3G06030.1 30.872 149 80 6 87 222 65 203 9.24e-15 73.2
Msa0218070 AT5G50860.2 33.557 149 86 4 80 222 104 245 1.06e-14 72.8
Msa0218070 AT5G50860.1 34.899 149 84 5 80 222 104 245 1.13e-14 72.8
Msa0218070 AT4G10010.2 31.646 158 90 7 75 222 138 287 1.48e-14 72.4
Msa0218070 AT1G66750.1 30.201 149 89 4 80 222 3 142 1.54e-14 72.0
Msa0218070 AT5G39420.1 31.724 145 86 4 84 222 99 236 2.79e-14 71.6
Msa0218070 AT5G01810.1 31.469 143 79 6 88 222 10 141 3.43e-14 71.2
Msa0218070 AT5G01810.2 31.469 143 79 6 88 222 10 141 3.43e-14 71.2
Msa0218070 AT5G01810.3 31.469 143 79 6 88 222 10 141 3.60e-14 70.9
Msa0218070 AT1G73690.1 32.237 152 77 6 83 222 3 140 3.75e-14 70.9
Msa0218070 AT1G18670.1 34.483 145 82 5 84 222 125 262 3.77e-14 71.2
Msa0218070 AT1G18670.3 34.483 145 82 5 84 222 125 262 3.96e-14 71.2
Msa0218070 AT2G46070.3 35.766 137 73 6 94 222 79 208 4.09e-14 70.9
Msa0218070 AT2G46070.1 35.766 137 73 6 94 222 45 174 4.69e-14 70.5
Msa0218070 AT1G09000.2 28.378 148 83 5 88 222 67 204 4.77e-14 70.9
Msa0218070 AT1G09000.1 28.378 148 83 5 88 222 67 204 5.01e-14 70.9
Msa0218070 AT2G46070.2 35.766 137 73 6 94 222 45 174 5.25e-14 70.1
Msa0218070 AT1G53050.2 34.667 150 75 5 84 222 128 265 5.42e-14 70.9
Msa0218070 AT1G53050.1 34.667 150 75 5 84 222 128 265 5.42e-14 70.9
Msa0218070 AT3G45640.1 30.827 133 81 3 96 222 44 171 6.27e-14 70.1
Msa0218070 AT1G59580.2 31.655 139 84 3 90 222 32 165 6.92e-14 70.1
Msa0218070 AT1G59580.1 31.655 139 84 3 90 222 32 165 6.92e-14 70.1
Msa0218070 AT1G07880.1 33.835 133 77 3 96 222 39 166 9.65e-14 68.6
Msa0218070 AT1G07880.2 36.842 133 73 4 96 222 39 166 1.18e-13 69.3
Msa0218070 AT4G36450.1 33.813 139 81 3 90 222 32 165 1.25e-13 69.3
Msa0218070 AT2G30360.1 29.944 177 85 7 56 222 5 152 1.55e-13 69.3
Msa0218070 AT5G44290.7 32.432 148 87 5 81 222 128 268 2.58e-13 68.6
Msa0218070 AT1G74330.3 31.724 145 86 4 84 222 115 252 2.94e-13 68.6
Msa0218070 AT1G54960.1 26.966 178 99 7 66 222 36 203 3.12e-13 68.6
Msa0218070 AT1G54960.2 26.966 178 99 7 66 222 36 203 3.12e-13 68.6
Msa0218070 AT5G44290.6 32.432 148 87 5 81 222 128 268 3.90e-13 68.2
Msa0218070 AT5G44290.2 32.432 148 87 5 81 222 128 268 4.36e-13 68.2
Msa0218070 AT5G44290.5 32.432 148 87 5 81 222 128 268 4.36e-13 68.2
Msa0218070 AT5G44290.4 32.432 148 87 5 81 222 128 268 4.36e-13 68.2
Msa0218070 AT5G44290.1 32.432 148 87 5 81 222 128 268 4.36e-13 68.2
Msa0218070 AT5G44290.3 32.432 148 87 5 81 222 128 268 4.36e-13 68.2
Msa0218070 AT1G74330.2 33.793 145 83 5 84 222 115 252 7.76e-13 67.4
Msa0218070 AT1G74330.1 33.793 145 83 5 84 222 115 252 7.79e-13 67.4
Msa0218070 AT1G09600.1 31.210 157 89 6 67 217 146 289 2.87e-12 65.9
Msa0218070 AT4G14580.1 33.803 142 75 7 90 222 21 152 2.89e-12 65.5
Msa0218070 AT2G26980.1 33.333 147 78 6 85 222 9 144 3.03e-12 65.5
Msa0218070 AT2G26980.3 33.333 147 78 6 85 222 9 144 3.27e-12 65.5
Msa0218070 AT2G26980.4 33.333 147 78 6 85 222 19 154 3.64e-12 65.5
Msa0218070 AT1G53570.7 30.968 155 87 7 78 222 202 346 5.06e-12 65.1
Msa0218070 AT1G53570.6 30.968 155 87 7 78 222 202 346 5.20e-12 65.1
Msa0218070 AT5G45820.1 33.099 142 74 5 90 222 12 141 5.69e-12 64.7
Msa0218070 AT2G26980.2 31.973 147 80 5 85 222 9 144 6.00e-12 63.9
Msa0218070 AT2G26980.5 33.333 147 78 6 85 222 9 144 6.20e-12 64.7
Msa0218070 AT1G53570.1 30.968 155 87 7 78 222 202 346 6.48e-12 64.7
Msa0218070 AT1G53570.5 30.968 155 87 7 78 222 202 346 6.70e-12 64.7
Msa0218070 AT1G53570.2 30.968 155 87 7 78 222 202 346 6.73e-12 64.7
Msa0218070 AT1G53570.3 30.968 155 87 7 78 222 202 346 6.73e-12 64.7
Msa0218070 AT1G53570.4 30.968 155 87 7 78 222 202 346 7.05e-12 64.7
Msa0218070 AT1G30270.1 32.394 142 76 5 90 222 31 161 9.10e-12 64.3
Msa0218070 AT4G24400.1 31.690 142 77 5 90 222 9 139 9.82e-12 63.9
Msa0218070 AT4G24400.2 31.690 142 77 5 90 222 9 139 9.90e-12 63.9
Msa0218070 AT1G03740.1 28.492 179 106 7 59 222 173 344 1.03e-11 64.3
Msa0218070 AT1G30270.2 32.394 142 76 5 90 222 31 161 1.08e-11 63.9
Msa0218070 AT1G67580.1 31.579 133 78 5 96 222 412 537 1.26e-11 63.9
Msa0218070 AT1G67580.2 31.579 133 78 5 96 222 412 537 1.26e-11 63.9
Msa0218070 AT5G45810.1 31.690 142 76 6 90 222 28 157 1.76e-11 63.2
Msa0218070 AT3G01085.3 26.897 145 93 3 84 222 109 246 1.81e-11 63.5
Msa0218070 AT3G01085.1 26.897 145 93 3 84 222 109 246 1.82e-11 63.5
Msa0218070 AT3G01085.2 26.897 145 93 3 84 222 109 246 1.82e-11 63.5
Msa0218070 AT5G10930.1 30.263 152 82 6 83 222 3 142 2.69e-11 62.8
Msa0218070 AT3G08720.1 29.927 137 78 6 94 222 144 270 5.04e-11 62.0
Msa0218070 AT3G08720.3 29.927 137 78 6 94 222 144 270 5.04e-11 62.0
Msa0218070 AT3G08720.4 29.927 137 78 6 94 222 144 270 5.04e-11 62.0
Msa0218070 AT3G08720.2 29.927 137 78 6 94 222 144 270 5.04e-11 62.0
Msa0218070 AT1G03740.2 31.724 145 86 5 84 222 207 344 5.68e-11 62.0
Msa0218070 AT3G08730.1 26.984 189 110 8 44 222 94 264 7.51e-11 61.6
Msa0218070 AT3G08730.2 26.984 189 110 8 44 222 94 264 7.51e-11 61.6

Find 64 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATAACTTACCAAAAGATTT+TGG 0.078191 2_1:+65100113 None:intergenic
CATGTTAACTTCAGATCTTC+AGG 0.253544 2_1:-65100189 Msa0218070:intron
AAACAAGCCCACACCCGTTT+AGG 0.318064 2_1:+65101119 None:intergenic
CGTGAAGCCCCAAAATCTTT+TGG 0.331667 2_1:-65100122 None:intergenic
TGCTTCAAGGAAATTACGTT+TGG 0.345363 2_1:+65100442 None:intergenic
GTGCTTGTTGCGGTGGTTGA+TGG 0.375998 2_1:+65103030 None:intergenic
GGCAAACTCCTGGCACAGTA+TGG 0.379893 2_1:+65100152 None:intergenic
GAAATGGCCACCATGCCGTT+GGG 0.382034 2_1:-65103065 Msa0218070:CDS
CATAGATCAGGGGCATTCTC+TGG 0.400773 2_1:+65100314 None:intergenic
AGAACTCGGTACATCGTCTC+AGG 0.408870 2_1:+65100358 None:intergenic
TATATAATTTCACATAGATC+AGG 0.418201 2_1:+65100302 None:intergenic
GTGAAAACGACGGCGGTTAA+CGG 0.434979 2_1:-65102945 Msa0218070:CDS
GTTGTTGGCACGGGATCATT+CGG 0.438459 2_1:-65100704 Msa0218070:CDS
TGAAATGGCCACCATGCCGT+TGG 0.466146 2_1:-65103066 Msa0218070:exon
ACGGCGGTTAACGGCGGCTC+CGG 0.466148 2_1:-65102936 Msa0218070:CDS
GCTTCAAGGAAATTACGTTT+GGG 0.466648 2_1:+65100443 None:intergenic
GGTTAACGGCGGCTCCGGTA+TGG 0.472476 2_1:-65102931 Msa0218070:CDS
AACTGTAACGGCGGTGGTAA+CGG 0.473438 2_1:+65102996 None:intergenic
CACATAATCTCAACCACCAT+TGG 0.475881 2_1:-65101158 Msa0218070:CDS
TGAAACACAGGCAAAGTGCT+TGG 0.494079 2_1:-65100554 Msa0218070:intron
AGAGAAGAAACAGTGCTTCA+AGG 0.498038 2_1:+65100429 None:intergenic
TCCCGTGCCAACAACACGTT+CGG 0.500515 2_1:+65100712 None:intergenic
TACATGGCCGAACGTGTTGT+TGG 0.506279 2_1:-65100719 Msa0218070:CDS
CAGGCAAAGTGCTTGGAGAC+TGG 0.508036 2_1:-65100547 Msa0218070:CDS
CTTGCAGACAATCAGTTACA+TGG 0.508309 2_1:-65100735 Msa0218070:intron
TTTGGGGCTTCACGTCCCTA+TGG 0.509279 2_1:+65100131 None:intergenic
GGCCGAACGTGTTGTTGGCA+CGG 0.511433 2_1:-65100714 Msa0218070:CDS
CTGTGCCAGGAGTTTGCCAT+AGG 0.519690 2_1:-65100147 Msa0218070:CDS
GCCGAACGTGTTGTTGGCAC+GGG 0.521820 2_1:-65100713 Msa0218070:CDS
ATGTTAACTTCAGATCTTCA+GGG 0.522133 2_1:-65100188 Msa0218070:intron
GGTGGTAACGGTAACGGTAG+TGG 0.523081 2_1:+65103008 None:intergenic
GAGAGTGTAGCTTTCTGAAA+TGG 0.525198 2_1:-65103081 Msa0218070:exon
CTTGGAGACTGGTGAGGCAG+TGG 0.528534 2_1:-65100536 Msa0218070:CDS
GACAGTAATGATGCAGTCAC+TGG 0.530679 2_1:-65101182 Msa0218070:CDS
CTCCGGTATGGAGACTGATA+AGG 0.535528 2_1:-65102919 Msa0218070:intron
TGAGCTTGTTGTAACTGTAA+CGG 0.537989 2_1:+65102984 None:intergenic
AGCTCAAACCGTGAATGAGC+CGG 0.545354 2_1:-65102967 Msa0218070:CDS
GCATACATCCATACTGTGCC+AGG 0.545838 2_1:-65100160 Msa0218070:CDS
CACCTTATCAGTCTCCATAC+CGG 0.552206 2_1:+65102917 None:intergenic
GTGATGTGGCCCCAACGGCA+TGG 0.554010 2_1:+65103055 None:intergenic
ATAATCTCAACCACCATTGG+AGG 0.560555 2_1:-65101155 Msa0218070:CDS
ACGTCCCTATGGCAAACTCC+TGG 0.573471 2_1:+65100142 None:intergenic
ATATAATTTCACATAGATCA+GGG 0.581499 2_1:+65100303 None:intergenic
TGTTGTAACTGTAACGGCGG+TGG 0.583386 2_1:+65102990 None:intergenic
GGTAGTGGTGCTTGTTGCGG+TGG 0.587513 2_1:+65103023 None:intergenic
TGAGGCAGTGGCTATAAAGA+AGG 0.596681 2_1:-65100524 Msa0218070:CDS
AAATGGCCACCATGCCGTTG+GGG 0.597985 2_1:-65103064 Msa0218070:CDS
CTTGTTGCGGTGGTTGATGG+TGG 0.598310 2_1:+65103033 None:intergenic
AACGGTAGTGGTGCTTGTTG+CGG 0.598923 2_1:+65103020 None:intergenic
TGGTGGTGATGTGGCCCCAA+CGG 0.608534 2_1:+65103050 None:intergenic
TGTGCCAGGAGTTTGCCATA+GGG 0.616642 2_1:-65100146 Msa0218070:CDS
ACTGCAGTTAATGCGCGTGA+TGG 0.625912 2_1:-65100470 Msa0218070:CDS
GGTGGTTGATGGTGGTGATG+TGG 0.637495 2_1:+65103041 None:intergenic
TGTTAACTTCAGATCTTCAG+GGG 0.648931 2_1:-65100187 Msa0218070:intron
TATAATTTCACATAGATCAG+GGG 0.651922 2_1:+65100304 None:intergenic
AGAAGGTATTGCAAGACAAG+CGG 0.653012 2_1:-65100507 Msa0218070:CDS
AACGGCGGTGGTAACGGTAA+CGG 0.656919 2_1:+65103002 None:intergenic
GAATGAGCCGGTGAAAACGA+CGG 0.659287 2_1:-65102955 Msa0218070:CDS
AAAACGACGGCGGTTAACGG+CGG 0.667392 2_1:-65102942 Msa0218070:CDS
GAACTCGGTACATCGTCTCA+GGG 0.674810 2_1:+65100359 None:intergenic
ATGTGGCCCCAACGGCATGG+TGG 0.682247 2_1:+65103058 None:intergenic
GCTTGTTGTAACTGTAACGG+CGG 0.690809 2_1:+65102987 None:intergenic
AAAGTGCTTGGAGACTGGTG+AGG 0.724717 2_1:-65100542 Msa0218070:CDS
TGAGCCGGTGAAAACGACGG+CGG 0.783984 2_1:-65102952 Msa0218070:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAAATAGATCTTTAATA+TGG + chr2_1:65102054-65102073 None:intergenic 10.0%
!!! AAAATTATCTTTTTTAATAG+AGG - chr2_1:65102276-65102295 Msa0218070:intron 10.0%
!!! AATTGAAGTAATAAATTTTA+AGG - chr2_1:65102630-65102649 Msa0218070:intron 10.0%
!! AAACCATAATTACTATTATT+AGG + chr2_1:65100579-65100598 None:intergenic 15.0%
!!! AATTTTTTTATTGTGCTTTT+AGG - chr2_1:65100658-65100677 Msa0218070:intron 15.0%
!!! ATTTTTTTATTGTGCTTTTA+GGG - chr2_1:65100659-65100678 Msa0218070:intron 15.0%
!! AAAATGAAAAATTGAAAATG+TGG - chr2_1:65100756-65100775 Msa0218070:intron 15.0%
!!! GAATATTGTTTCTTTAATTT+AGG - chr2_1:65100778-65100797 Msa0218070:intron 15.0%
!!! TTAATTTTTTTTTGGGTTTA+TGG + chr2_1:65101100-65101119 None:intergenic 15.0%
!!! ATCCACATTAATTTTTTTTT+GGG + chr2_1:65101107-65101126 None:intergenic 15.0%
!!! AATCCACATTAATTTTTTTT+TGG + chr2_1:65101108-65101127 None:intergenic 15.0%
!!! ATTGTTTTTTATTGACAAAA+AGG - chr2_1:65101334-65101353 Msa0218070:intron 15.0%
!! ATAGTTTCTATTTATATCAT+TGG - chr2_1:65101390-65101409 Msa0218070:intron 15.0%
!! TATTTATTACTATTAATTGC+AGG - chr2_1:65102065-65102084 Msa0218070:intron 15.0%
!! AGTATTAGTCAATTAAAAAT+TGG - chr2_1:65102318-65102337 Msa0218070:intron 15.0%
!! GTATTAGTCAATTAAAAATT+GGG - chr2_1:65102319-65102338 Msa0218070:intron 15.0%
!! CCTATTAATATTAATTAGAT+AGG + chr2_1:65102359-65102378 None:intergenic 15.0%
!! CCTATCTAATTAATATTAAT+AGG - chr2_1:65102356-65102375 Msa0218070:intron 15.0%
!!! CTTCAAAATTATACTTTTTA+AGG + chr2_1:65102437-65102456 None:intergenic 15.0%
!! TCACTATTTGAAAATTGTAT+AGG - chr2_1:65100811-65100830 Msa0218070:intron 20.0%
!!! ATATTGTTGTAGAAGTTTTT+GGG - chr2_1:65100896-65100915 Msa0218070:intron 20.0%
!!! TAAGGTAGTTTGTTTTAATT+TGG - chr2_1:65100972-65100991 Msa0218070:intron 20.0%
!! AACCCAAAAAAAAATTAATG+TGG - chr2_1:65101102-65101121 Msa0218070:intron 20.0%
!!! TAATATTGTGATTTTTGTGT+TGG - chr2_1:65101300-65101319 Msa0218070:intron 20.0%
!!! TATTGCTTGTAATGTTATTT+TGG - chr2_1:65101366-65101385 Msa0218070:intron 20.0%
!! TCTTGTCATATAAGTTATAA+CGG - chr2_1:65101690-65101709 Msa0218070:intron 20.0%
!! ATAACTTATCTTCAATAAGT+CGG + chr2_1:65101922-65101941 None:intergenic 20.0%
!!! TGTTCTTTTATTTCTGTTTT+AGG - chr2_1:65102016-65102035 Msa0218070:intron 20.0%
!! AATATTGACAATAACAGTTA+TGG - chr2_1:65102490-65102509 Msa0218070:intron 20.0%
!! ATATTGACAATAACAGTTAT+GGG - chr2_1:65102491-65102510 Msa0218070:intron 20.0%
!! GCATAAATTTATGAAACAAA+TGG + chr2_1:65102528-65102547 None:intergenic 20.0%
!! ATATAATTTCACATAGATCA+GGG + chr2_1:65102990-65103009 None:intergenic 20.0%
!! TATATAATTTCACATAGATC+AGG + chr2_1:65102991-65103010 None:intergenic 20.0%
! CGACCTAATAATAGTAATTA+TGG - chr2_1:65100573-65100592 Msa0218070:intron 25.0%
! TGTTGAAAAATTGAAGTTCA+TGG - chr2_1:65100694-65100713 Msa0218070:CDS 25.0%
!! CATATTGTTGTAGAAGTTTT+TGG - chr2_1:65100895-65100914 Msa0218070:intron 25.0%
!! GAAAGTAGTGTTTTCAATTA+AGG - chr2_1:65100954-65100973 Msa0218070:intron 25.0%
!!! TGTTTTAATTTGGAATTGTC+TGG - chr2_1:65100982-65101001 Msa0218070:intron 25.0%
!!! AAATGGTTTTAATTTGCTTC+AGG - chr2_1:65101452-65101471 Msa0218070:intron 25.0%
!!! AATGGTTTTAATTTGCTTCA+GGG - chr2_1:65101453-65101472 Msa0218070:intron 25.0%
! ACTTCTGACAAATTAAGATA+AGG + chr2_1:65101780-65101799 None:intergenic 25.0%
!! ATAAGTTATTAAGCCAGTTT+TGG - chr2_1:65101933-65101952 Msa0218070:intron 25.0%
! TAATAACTAACTACCAAAAC+TGG + chr2_1:65101949-65101968 None:intergenic 25.0%
!! AAAAATTGGGTTATTTCCTA+CGG - chr2_1:65102332-65102351 Msa0218070:intron 25.0%
!! TTTTCCCTATTCGAAATTTT+TGG - chr2_1:65102395-65102414 Msa0218070:intron 25.0%
!!! TTTTTAAGGTTCAACAAATG+AGG + chr2_1:65102423-65102442 None:intergenic 25.0%
! TTTATTGAAATAGCATTGCA+AGG + chr2_1:65102471-65102490 None:intergenic 25.0%
! TTACTTCAATTATCTTTACC+TGG + chr2_1:65102621-65102640 None:intergenic 25.0%
!!! TATTGTAGTGTTTTAGAACT+CGG + chr2_1:65102949-65102968 None:intergenic 25.0%
! TATAATTTCACATAGATCAG+GGG + chr2_1:65102989-65103008 None:intergenic 25.0%
! TTATATACATACCAAGTATG+AGG - chr2_1:65103004-65103023 Msa0218070:CDS 25.0%
AGTGGTTGAGAAAAAGAAAA+AGG - chr2_1:65100729-65100748 Msa0218070:CDS 30.0%
ATCATGTTGTCTTAACCTAA+CGG - chr2_1:65100845-65100864 Msa0218070:intron 30.0%
!! ATTATCTCCTAGGTTGTTTT+AGG - chr2_1:65100932-65100951 Msa0218070:intron 30.0%
!!! AACCTATATTGGGTTTTGAA+CGG + chr2_1:65101038-65101057 None:intergenic 30.0%
! ATTTTAAATGTGGAGATGTG+GGG + chr2_1:65101206-65101225 None:intergenic 30.0%
! CATTTTAAATGTGGAGATGT+GGG + chr2_1:65101207-65101226 None:intergenic 30.0%
! ACATTTTAAATGTGGAGATG+TGG + chr2_1:65101208-65101227 None:intergenic 30.0%
! CCATTTGAAACAACAAATCA+AGG + chr2_1:65101438-65101457 None:intergenic 30.0%
!! CCTTGATTTGTTGTTTCAAA+TGG - chr2_1:65101435-65101454 Msa0218070:intron 30.0%
!! TTCACAAGAAAGCATCAAAT+GGG + chr2_1:65101543-65101562 None:intergenic 30.0%
!! ATTCACAAGAAAGCATCAAA+TGG + chr2_1:65101544-65101563 None:intergenic 30.0%
GGTATATGCACAAATAAAGT+AGG + chr2_1:65101575-65101594 None:intergenic 30.0%
ATAACGGTTCAAATTAATGC+AGG - chr2_1:65101706-65101725 Msa0218070:intron 30.0%
TCATACATGAGATATCTGAT+AGG - chr2_1:65101873-65101892 Msa0218070:intron 30.0%
ATTAACTCGATAAGCAATTC+TGG - chr2_1:65101965-65101984 Msa0218070:intron 30.0%
TTAACTCGATAAGCAATTCT+GGG - chr2_1:65101966-65101985 Msa0218070:intron 30.0%
! AGCAATTCTGGGTATTTTAT+AGG - chr2_1:65101977-65101996 Msa0218070:intron 30.0%
TTAAGACTCTAGTACAAAGA+TGG + chr2_1:65102200-65102219 None:intergenic 30.0%
TATTAATTAGATAGGACCGT+AGG + chr2_1:65102351-65102370 None:intergenic 30.0%
AAATTTCGAATAGGGAAAAG+AGG + chr2_1:65102394-65102413 None:intergenic 30.0%
GGAACCAAAAATTTCGAATA+GGG + chr2_1:65102402-65102421 None:intergenic 30.0%
AGGAACCAAAAATTTCGAAT+AGG + chr2_1:65102403-65102422 None:intergenic 30.0%
ATGTTAACTTCAGATCTTCA+GGG - chr2_1:65103102-65103121 Msa0218070:five_prime_UTR 30.0%
!! TGAGCTTGTTGTAACTGTAA+CGG + chr2_1:65100309-65100328 None:intergenic 35.0%
AACGAAAATTAACAGATCGC+CGG + chr2_1:65100488-65100507 None:intergenic 35.0%
!!! TCATGGAGTTTTTTGTTGAG+TGG - chr2_1:65100711-65100730 Msa0218070:CDS 35.0%
TTTAACTACACACAACCGTT+AGG + chr2_1:65100863-65100882 None:intergenic 35.0%
!!! TGTTGTAGAAGTTTTTGGGT+TGG - chr2_1:65100900-65100919 Msa0218070:intron 35.0%
GATGTAGCAAATTATCTCCT+AGG - chr2_1:65100922-65100941 Msa0218070:intron 35.0%
CTACTTTCCTAAAACAACCT+AGG + chr2_1:65100942-65100961 None:intergenic 35.0%
TACCAACTAAGTTGTGCTTA+CGG + chr2_1:65101136-65101155 None:intergenic 35.0%
! AGGCTCACACATTTTAAATG+TGG + chr2_1:65101216-65101235 None:intergenic 35.0%
GTAAAGTAGTCTAATGACTG+AGG + chr2_1:65101236-65101255 None:intergenic 35.0%
! TGTGATTTTTGTGTTGGAGT+TGG - chr2_1:65101306-65101325 Msa0218070:intron 35.0%
!! CATTGGTTCCTTGCTTAAAA+TGG - chr2_1:65101407-65101426 Msa0218070:intron 35.0%
! AGGAAACTCCATTTTAAGCA+AGG + chr2_1:65101418-65101437 None:intergenic 35.0%
TGATGCTTTCTTGTGAATTG+TGG - chr2_1:65101546-65101565 Msa0218070:intron 35.0%
GTAGTCAAGACTCAAAACTT+TGG + chr2_1:65101807-65101826 None:intergenic 35.0%
TGACTCAGCAAAGTCATTAA+TGG + chr2_1:65101856-65101875 None:intergenic 35.0%
! ATCATTCGGAATTGTTTTCC+AGG - chr2_1:65102600-65102619 Msa0218070:intron 35.0%
GCTTCAAGGAAATTACGTTT+GGG + chr2_1:65102850-65102869 None:intergenic 35.0%
TGCTTCAAGGAAATTACGTT+TGG + chr2_1:65102851-65102870 None:intergenic 35.0%
!! AGACGAACTTTTTCTGAACT+TGG - chr2_1:65102895-65102914 Msa0218070:intron 35.0%
!! ACTTTTTCTGAACTTGGTCA+TGG - chr2_1:65102901-65102920 Msa0218070:intron 35.0%
AATAGAGAAGACCTCATACT+TGG + chr2_1:65103018-65103037 None:intergenic 35.0%
! GGTCTTCTCTATTTGCATTT+TGG - chr2_1:65103025-65103044 Msa0218070:CDS 35.0%
!! TCTCTATTTGCATTTTGGTC+TGG - chr2_1:65103030-65103049 Msa0218070:CDS 35.0%
CATGTTAACTTCAGATCTTC+AGG - chr2_1:65103101-65103120 Msa0218070:five_prime_UTR 35.0%
TGTTAACTTCAGATCTTCAG+GGG - chr2_1:65103103-65103122 Msa0218070:five_prime_UTR 35.0%
! GAGAGTGTAGCTTTCTGAAA+TGG - chr2_1:65100209-65100228 Msa0218070:intron 40.0%
TAACTACAGTTCTCGTTGCT+TGG - chr2_1:65100418-65100437 Msa0218070:CDS 40.0%
AGAAAGACTTTGAACTTCGC+CGG + chr2_1:65100549-65100568 None:intergenic 40.0%
CGAAGTTCAAAGTCTTTCTC+TGG - chr2_1:65100550-65100569 Msa0218070:intron 40.0%
!!! CTATATTGGGTTTTGAACGG+AGG + chr2_1:65101035-65101054 None:intergenic 40.0%
CTCCGTTCAAAACCCAATAT+AGG - chr2_1:65101033-65101052 Msa0218070:intron 40.0%
ATCGGGTTCGAACCTATATT+GGG + chr2_1:65101048-65101067 None:intergenic 40.0%
TATCGGGTTCGAACCTATAT+TGG + chr2_1:65101049-65101068 None:intergenic 40.0%
TTCGAACCCGATATAGTTTG+AGG - chr2_1:65101056-65101075 Msa0218070:intron 40.0%
AACACCGCCTCAAACTATAT+CGG + chr2_1:65101066-65101085 None:intergenic 40.0%
TTCCGTAAGCACAACTTAGT+TGG - chr2_1:65101131-65101150 Msa0218070:intron 40.0%
TGCAAGGACAATATTACGCA+GGG - chr2_1:65101158-65101177 Msa0218070:CDS 40.0%
GTTAAGTGTGAAGAGCTCAA+AGG + chr2_1:65101596-65101615 None:intergenic 40.0%
CTTCACACTTAACCCATCTT+TGG - chr2_1:65101603-65101622 Msa0218070:intron 40.0%
CAGAACGCAAAACCAAAGAT+GGG + chr2_1:65101618-65101637 None:intergenic 40.0%
TCAGAACGCAAAACCAAAGA+TGG + chr2_1:65101619-65101638 None:intergenic 40.0%
!!! CTTTGGTTTTGCGTTCTGAT+TGG - chr2_1:65101620-65101639 Msa0218070:intron 40.0%
TAGAGCTTAACCAGCAGAAA+TGG + chr2_1:65101656-65101675 None:intergenic 40.0%
CACATAATCTCAACCACCAT+TGG - chr2_1:65102132-65102151 Msa0218070:intron 40.0%
ATAATCTCAACCACCATTGG+AGG - chr2_1:65102135-65102154 Msa0218070:intron 40.0%
GCAAAAACGGTGAACCTAAA+CGG - chr2_1:65102157-65102176 Msa0218070:intron 40.0%
CAAAAACGGTGAACCTAAAC+GGG - chr2_1:65102158-65102177 Msa0218070:intron 40.0%
CTTGCAGACAATCAGTTACA+TGG - chr2_1:65102555-65102574 Msa0218070:intron 40.0%
AAAACGGTAGATACACAACC+CGG + chr2_1:65102712-65102731 None:intergenic 40.0%
!! ACCGTTTTGATCTGAAACAC+AGG - chr2_1:65102724-65102743 Msa0218070:intron 40.0%
GCCTGTGTTTCAGATCAAAA+CGG + chr2_1:65102728-65102747 None:intergenic 40.0%
!! AGAAGGTATTGCAAGACAAG+CGG - chr2_1:65102783-65102802 Msa0218070:intron 40.0%
AGAGAAGAAACAGTGCTTCA+AGG + chr2_1:65102864-65102883 None:intergenic 40.0%
!! GCTTGTTGTAACTGTAACGG+CGG + chr2_1:65100306-65100325 None:intergenic 45.0%
CACCTTATCAGTCTCCATAC+CGG + chr2_1:65100376-65100395 None:intergenic 45.0%
AAATTAACAGATCGCCGGTG+AGG + chr2_1:65100483-65100502 None:intergenic 45.0%
GAACCCGATATAGTTTGAGG+CGG - chr2_1:65101059-65101078 Msa0218070:intron 45.0%
ACACCGCCTCAAACTATATC+GGG + chr2_1:65101065-65101084 None:intergenic 45.0%
!! CAACTTAGTTGGTAGCTGCA+AGG - chr2_1:65101142-65101161 Msa0218070:CDS 45.0%
CTGCAAGGACAATATTACGC+AGG - chr2_1:65101157-65101176 Msa0218070:CDS 45.0%
GCAAGGACAATATTACGCAG+GGG - chr2_1:65101159-65101178 Msa0218070:CDS 45.0%
GACAATATTACGCAGGGGTT+GGG - chr2_1:65101164-65101183 Msa0218070:CDS 45.0%
ACAATATTACGCAGGGGTTG+GGG - chr2_1:65101165-65101184 Msa0218070:CDS 45.0%
!! TGGTTTTGCGTTCTGATTGG+TGG - chr2_1:65101623-65101642 Msa0218070:intron 45.0%
!! TGGCTGTTTTCCATTTCTGC+TGG - chr2_1:65101643-65101662 Msa0218070:intron 45.0%
GACAGTAATGATGCAGTCAC+TGG - chr2_1:65102108-65102127 Msa0218070:intron 45.0%
ACCACCATTGGAGGCAAAAA+CGG - chr2_1:65102144-65102163 Msa0218070:intron 45.0%
!! TTCACCGTTTTTGCCTCCAA+TGG + chr2_1:65102151-65102170 None:intergenic 45.0%
GAATAGGGAAAAGAGGCAGA+GGG + chr2_1:65102387-65102406 None:intergenic 45.0%
CTCATATTTCTTCACACCCC+CGG - chr2_1:65102690-65102709 Msa0218070:intron 45.0%
TCATATTTCTTCACACCCCC+GGG - chr2_1:65102691-65102710 Msa0218070:intron 45.0%
AAACGGTAGATACACAACCC+GGG + chr2_1:65102711-65102730 None:intergenic 45.0%
! TGAAACACAGGCAAAGTGCT+TGG - chr2_1:65102736-65102755 Msa0218070:intron 45.0%
TGAGGCAGTGGCTATAAAGA+AGG - chr2_1:65102766-65102785 Msa0218070:intron 45.0%
!! AAAAATAAATAATATTAATG+TGG - chr2_1:65101260-65101279 Msa0218070:intron 5.0%
!!! TTTTTTTTTTTTTTTTTTTG+AGG - chr2_1:65102235-65102254 Msa0218070:intron 5.0%
!!! TTTTTTTTTTTTTTTTTTGA+GGG - chr2_1:65102236-65102255 Msa0218070:intron 5.0%
! AACGGTAGTGGTGCTTGTTG+CGG + chr2_1:65100273-65100292 None:intergenic 50.0%
AACTGTAACGGCGGTGGTAA+CGG + chr2_1:65100297-65100316 None:intergenic 50.0%
TGTTGTAACTGTAACGGCGG+TGG + chr2_1:65100303-65100322 None:intergenic 50.0%
AGCTCAAACCGTGAATGAGC+CGG - chr2_1:65100323-65100342 Msa0218070:CDS 50.0%
!!! CGTTTTCACCGGCTCATTCA+CGG + chr2_1:65100334-65100353 None:intergenic 50.0%
GAATGAGCCGGTGAAAACGA+CGG - chr2_1:65100335-65100354 Msa0218070:CDS 50.0%
!! TTAACCGCCGTCGTTTTCAC+CGG + chr2_1:65100345-65100364 None:intergenic 50.0%
GTGAAAACGACGGCGGTTAA+CGG - chr2_1:65100345-65100364 Msa0218070:CDS 50.0%
CTCCGGTATGGAGACTGATA+AGG - chr2_1:65100371-65100390 Msa0218070:CDS 50.0%
TGATCCGCTGTGTAAAGCTC+CGG - chr2_1:65100449-65100468 Msa0218070:CDS 50.0%
TCAACCGGAGCTTTACACAG+CGG + chr2_1:65100456-65100475 None:intergenic 50.0%
!!! TGGGTTTTGAACGGAGGTGA+AGG + chr2_1:65101029-65101048 None:intergenic 50.0%
GGACAATATTACGCAGGGGT+TGG - chr2_1:65101163-65101182 Msa0218070:CDS 50.0%
ATGTGGAGATGTGGGGAGTT+TGG + chr2_1:65101199-65101218 None:intergenic 50.0%
!! ACCGTTTTTGCCTCCAATGG+TGG + chr2_1:65102148-65102167 None:intergenic 50.0%
AAACAAGCCCACACCCGTTT+AGG + chr2_1:65102174-65102193 None:intergenic 50.0%
CGAATAGGGAAAAGAGGCAG+AGG + chr2_1:65102388-65102407 None:intergenic 50.0%
TACATGGCCGAACGTGTTGT+TGG - chr2_1:65102571-65102590 Msa0218070:intron 50.0%
GTTGTTGGCACGGGATCATT+CGG - chr2_1:65102586-65102605 Msa0218070:intron 50.0%
AACGGTAGATACACAACCCG+GGG + chr2_1:65102710-65102729 None:intergenic 50.0%
! AAAGTGCTTGGAGACTGGTG+AGG - chr2_1:65102748-65102767 Msa0218070:intron 50.0%
ACTGCAGTTAATGCGCGTGA+TGG - chr2_1:65102820-65102839 Msa0218070:intron 50.0%
GAACTCGGTACATCGTCTCA+GGG + chr2_1:65102934-65102953 None:intergenic 50.0%
AGAACTCGGTACATCGTCTC+AGG + chr2_1:65102935-65102954 None:intergenic 50.0%
!! CATAGATCAGGGGCATTCTC+TGG + chr2_1:65102979-65102998 None:intergenic 50.0%
GCATACATCCATACTGTGCC+AGG - chr2_1:65103130-65103149 Msa0218070:five_prime_UTR 50.0%
TGTGCCAGGAGTTTGCCATA+GGG - chr2_1:65103144-65103163 Msa0218070:five_prime_UTR 50.0%
TGAAATGGCCACCATGCCGT+TGG - chr2_1:65100224-65100243 Msa0218070:intron 55.0%
GAAATGGCCACCATGCCGTT+GGG - chr2_1:65100225-65100244 Msa0218070:intron 55.0%
AAATGGCCACCATGCCGTTG+GGG - chr2_1:65100226-65100245 Msa0218070:intron 55.0%
!! GGTGGTTGATGGTGGTGATG+TGG + chr2_1:65100252-65100271 None:intergenic 55.0%
!! CTTGTTGCGGTGGTTGATGG+TGG + chr2_1:65100260-65100279 None:intergenic 55.0%
! GTGCTTGTTGCGGTGGTTGA+TGG + chr2_1:65100263-65100282 None:intergenic 55.0%
!! GGTGGTAACGGTAACGGTAG+TGG + chr2_1:65100285-65100304 None:intergenic 55.0%
!! AACGGCGGTGGTAACGGTAA+CGG + chr2_1:65100291-65100310 None:intergenic 55.0%
AAAACGACGGCGGTTAACGG+CGG - chr2_1:65100348-65100367 Msa0218070:CDS 55.0%
CTTCGCCGGAGAACAAACTC+CGG + chr2_1:65100535-65100554 None:intergenic 55.0%
ACGGTGAACCTAAACGGGTG+TGG - chr2_1:65102163-65102182 Msa0218070:intron 55.0%
CGGTGAACCTAAACGGGTGT+GGG - chr2_1:65102164-65102183 Msa0218070:intron 55.0%
TCCCGTGCCAACAACACGTT+CGG + chr2_1:65102581-65102600 None:intergenic 55.0%
ACGGTAGATACACAACCCGG+GGG + chr2_1:65102709-65102728 None:intergenic 55.0%
! CAGGCAAAGTGCTTGGAGAC+TGG - chr2_1:65102743-65102762 Msa0218070:intron 55.0%
GGCAAACTCCTGGCACAGTA+TGG + chr2_1:65103141-65103160 None:intergenic 55.0%
CTGTGCCAGGAGTTTGCCAT+AGG - chr2_1:65103143-65103162 Msa0218070:five_prime_UTR 55.0%
ACGTCCCTATGGCAAACTCC+TGG + chr2_1:65103151-65103170 None:intergenic 55.0%
! TTTGGGGCTTCACGTCCCTA+TGG + chr2_1:65103162-65103181 None:intergenic 55.0%
! TGGTGGTGATGTGGCCCCAA+CGG + chr2_1:65100243-65100262 None:intergenic 60.0%
! GGTAGTGGTGCTTGTTGCGG+TGG + chr2_1:65100270-65100289 None:intergenic 60.0%
TGAGCCGGTGAAAACGACGG+CGG - chr2_1:65100338-65100357 Msa0218070:CDS 60.0%
CTCCGGTTGAGCTTCCTCAC+CGG - chr2_1:65100466-65100485 Msa0218070:CDS 60.0%
! AGAAGCTCGAGTTGCCTCGC+CGG - chr2_1:65100513-65100532 Msa0218070:CDS 60.0%
! CCTCGCCGGAGTTTGTTCTC+CGG - chr2_1:65100527-65100546 Msa0218070:CDS 60.0%
! GGCCGAACGTGTTGTTGGCA+CGG - chr2_1:65102576-65102595 Msa0218070:intron 60.0%
! GCCGAACGTGTTGTTGGCAC+GGG - chr2_1:65102577-65102596 Msa0218070:intron 60.0%
CTTGGAGACTGGTGAGGCAG+TGG - chr2_1:65102754-65102773 Msa0218070:intron 60.0%
ATGTGGCCCCAACGGCATGG+TGG + chr2_1:65100235-65100254 None:intergenic 65.0%
GTGATGTGGCCCCAACGGCA+TGG + chr2_1:65100238-65100257 None:intergenic 65.0%
GGTTAACGGCGGCTCCGGTA+TGG - chr2_1:65100359-65100378 Msa0218070:CDS 65.0%
CGCCGGTGAGGAAGCTCAAC+CGG + chr2_1:65100471-65100490 None:intergenic 65.0%
CCGGAGAACAAACTCCGGCG+AGG + chr2_1:65100530-65100549 None:intergenic 65.0%
ACGGCGGTTAACGGCGGCTC+CGG - chr2_1:65100354-65100373 Msa0218070:CDS 70.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 65100123 65103189 65100123 ID=Msa0218070;Name=Msa0218070
chr2_1 mRNA 65100123 65103189 65100123 ID=Msa0218070-mRNA-1;Parent=Msa0218070;Name=Msa0218070-mRNA-1;_AED=0.02;_eAED=0.02;_QI=105|1|1|1|1|1|5|0|222
chr2_1 exon 65100123 65100197 65100123 ID=Msa0218070-mRNA-1:exon:18118;Parent=Msa0218070-mRNA-1
chr2_1 exon 65100294 65100566 65100294 ID=Msa0218070-mRNA-1:exon:18117;Parent=Msa0218070-mRNA-1
chr2_1 exon 65100691 65100750 65100691 ID=Msa0218070-mRNA-1:exon:18116;Parent=Msa0218070-mRNA-1
chr2_1 exon 65101133 65101225 65101133 ID=Msa0218070-mRNA-1:exon:18115;Parent=Msa0218070-mRNA-1
chr2_1 exon 65102920 65103189 65102920 ID=Msa0218070-mRNA-1:exon:18114;Parent=Msa0218070-mRNA-1
chr2_1 five_prime_UTR 65103085 65103189 65103085 ID=Msa0218070-mRNA-1:five_prime_utr;Parent=Msa0218070-mRNA-1
chr2_1 CDS 65102920 65103084 65102920 ID=Msa0218070-mRNA-1:cds;Parent=Msa0218070-mRNA-1
chr2_1 CDS 65101133 65101225 65101133 ID=Msa0218070-mRNA-1:cds;Parent=Msa0218070-mRNA-1
chr2_1 CDS 65100691 65100750 65100691 ID=Msa0218070-mRNA-1:cds;Parent=Msa0218070-mRNA-1
chr2_1 CDS 65100294 65100566 65100294 ID=Msa0218070-mRNA-1:cds;Parent=Msa0218070-mRNA-1
chr2_1 CDS 65100123 65100197 65100123 ID=Msa0218070-mRNA-1:cds;Parent=Msa0218070-mRNA-1
Gene Sequence

>Msa0218070

ATGGCCACCATGCCGTTGGGGCCACATCACCACCATCAACCACCGCAACAAGCACCACTACCGTTACCGTTACCACCGCCGTTACAGTTACAACAAGCTCAAACCGTGAATGAGCCGGTGAAAACGACGGCGGTTAACGGCGGCTCCGGTATGGAGACTGATAAGGAAATGTCAGCTAGTGTCGTTGACAGTAATGATGCAGTCACTGGTCACATAATCTCAACCACCATTGGAGGCAAAAACGGTGAACCTAAACGGACAATCAGTTACATGGCCGAACGTGTTGTTGGCACGGGATCATTCGGAATTGTTTTCCAGGCAAAGTGCTTGGAGACTGGTGAGGCAGTGGCTATAAAGAAGGTATTGCAAGACAAGCGGTACAAAAATCGTGAACTGCAGTTAATGCGCGTGATGGATCACCCAAACGTAATTTCCTTGAAGCACTGTTTCTTCTCTACAACAAGCAGAGACGAACTTTTTCTGAACTTGGTCATGGAGTATGTCCCTGAGACGATGTACCGAGTTCTAAAACACTACAATAATATCAACCAGAGAATGCCCCTGATCTATGTGAAATTATATACATACCAAATCTTCAGGGGACTAGCATACATCCATACTGTGCCAGGAGTTTGCCATAGGGACGTGAAGCCCCAAAATCTTTTG
Protein sequence

>Msa0218070

MATMPLGPHHHHQPPQQAPLPLPLPPPLQLQQAQTVNEPVKTTAVNGGSGMETDKEMSASVVDSNDAVTGHIISTTIGGKNGEPKRTISYMAERVVGTGSFGIVFQAKCLETGEAVAIKKVLQDKRYKNRELQLMRVMDHPNVISLKHCFFSTTSRDELFLNLVMEYVPETMYRVLKHYNNINQRMPLIYVKLYTYQIFRGLAYIHTVPGVCHRDVKPQNLL