AlfalfaGEDB | Msa0218410

M. sativa cultivar zhongmu-4 / Msa0218410

KEGG: -
GO: -
PFAM: PF00643.27

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0218410	KEH38906.1	82.791	215	37	0	5	219	1	215	3.18e-123	369
Msa0218410	KEH38906.1	89.669	242	25	0	223	464	1	242	1.95e-156	453

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0218410	sp\|Q1G3Q4\|RITF1_ARATH	40.000	140	73	5	9	148	8	136	2.70e-22	99.0
Msa0218410	sp\|Q1G3Q4\|RITF1_ARATH	40.000	140	73	5	227	366	8	136	8.08e-22	97.8

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0218410	A0A072VBE7	82.791	215	37	0	5	219	1	215	1.52e-123	369
Msa0218410	A0A072VBE7	89.669	242	25	0	223	464	1	242	9.32e-157	453

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TFs/TRs:

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Gene ID	Type	Classification
Msa0218410	TF	PLATZ

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0218410	MtrunA17_Chr2g0320561	89.300	243	26	0	222	464	1	243	5.51e-141	404
Msa0218410	MtrunA17_Chr2g0320561	88.889	216	24	0	4	219	1	216	6.20e-126	365
Msa0218410	MtrunA17_Chr2g0320551	85.526	152	22	0	222	373	1	152	1.82e-91	275
Msa0218410	MtrunA17_Chr2g0320551	79.605	152	31	0	4	155	1	152	1.54e-83	254
Msa0218410	MtrunA17_Chr2g0320521	59.016	244	87	3	221	464	2	232	3.37e-88	269
Msa0218410	MtrunA17_Chr2g0320521	65.464	194	65	1	3	196	2	193	1.21e-86	265
Msa0218410	MtrunA17_Chr4g0020811	73.510	151	40	0	3	153	2	152	3.70e-75	233
Msa0218410	MtrunA17_Chr4g0020811	71.523	151	43	0	221	371	2	152	2.05e-71	223
Msa0218410	MtrunA17_Chr2g0308401	73.333	150	40	0	4	153	1	150	3.79e-74	230
Msa0218410	MtrunA17_Chr2g0308401	71.333	150	43	0	222	371	1	150	1.80e-70	220

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0218410	AT1G43000.1	37.959	245	117	8	221	463	5	216	1.69e-36	134
Msa0218410	AT1G43000.1	46.897	145	67	3	3	147	5	139	9.58e-36	132
Msa0218410	AT1G43000.2	37.959	245	117	8	221	463	12	223	2.23e-36	134
Msa0218410	AT1G43000.2	46.897	145	67	3	3	147	12	146	1.11e-35	132
Msa0218410	AT1G21000.2	34.413	247	146	7	221	463	9	243	2.51e-36	134
Msa0218410	AT1G21000.2	45.890	146	69	5	3	148	9	144	3.47e-31	120
Msa0218410	AT1G21000.1	34.413	247	146	7	221	463	12	246	3.19e-36	134
Msa0218410	AT1G21000.1	45.890	146	69	5	3	148	12	147	4.13e-31	120
Msa0218410	AT1G76590.1	34.855	241	140	8	226	463	19	245	3.24e-34	129
Msa0218410	AT1G76590.1	44.366	142	69	5	8	149	19	150	1.47e-29	116

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Find 67 sgRNAs with CRISPR-Local

Find 535 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAGTCCCTATTGTGAC+CGG	0.541668	2_1:+65570521	None:intergenic
AAAGAAAAGGAATAGCTCAT+AGG	0.373915	2_1:-65563676	Msa0218410:CDS
AAGAAAAGGAATAGCTCATA+GGG	0.480707	2_1:-65563675	Msa0218410:CDS
AAGAAGATCATCATATAATG+AGG	0.589252	2_1:-65570676	Msa0218410:CDS
AAGAATGCTTTCTCTGTCAT+CGG	0.539585	2_1:+65563742	None:intergenic
AAGATCATCATATAATGAGG+TGG	0.649716	2_1:-65570673	Msa0218410:CDS
AATGTTGAACCTCTTAGATT+GGG	0.435133	2_1:+65564329	None:intergenic
AATGTTGAACCTCTTAGGTT+GGG	0.427697	2_1:+65570551	None:intergenic
AATTGATGGGAAAGAGGAAG+AGG	0.477013	2_1:-65563795	Msa0218410:CDS
AATTGATGGGAAAGAGGAAG+AGG	0.481751	2_1:-65570023	Msa0218410:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGATCATCATATAATGAGG+TGG	-	chr2_1:65564099-65564118	Msa0218410:intron	30.0%
CACATGTTTGTAAATTTCGA+TGG	+	chr2_1:65564131-65564150	None:intergenic	30.0%
CATCGAAATTTACAAACATG+TGG	-	chr2_1:65564129-65564148	Msa0218410:intron	30.0%
AGGAGTGGAATAAAATATAC+TGG	+	chr2_1:65564415-65564434	None:intergenic	30.0%
TTAGAAGACGTAAGAATTGA+TGG	-	chr2_1:65564735-65564754	Msa0218410:CDS	30.0%
TAGAAGACGTAAGAATTGAT+GGG	-	chr2_1:65564736-65564755	Msa0218410:CDS	30.0%
CAAGCCAAATTCAAAAAGAA+AGG	-	chr2_1:65564851-65564870	Msa0218410:intron	30.0%
AAAGAAAGGGAATAGCTTAT+AGG	-	chr2_1:65564865-65564884	Msa0218410:intron	30.0%
AAGAAAGGGAATAGCTTATA+GGG	-	chr2_1:65564866-65564885	Msa0218410:intron	30.0%
TTGACGATTAAATGTTCGAA+AGG	+	chr2_1:65564997-65565016	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	65563661	65571133	65563661	ID=Msa0218410;Name=Msa0218410
chr2_1	mRNA	65563661	65571133	65563661	ID=Msa0218410-mRNA-1;Parent=Msa0218410;Name=Msa0218410-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0\|0\|0\|0.85\|0.83\|0.85\|7\|0\|464
chr2_1	exon	65571128	65571133	65571128	ID=Msa0218410-mRNA-1:exon:18296;Parent=Msa0218410-mRNA-1
chr2_1	exon	65570862	65571041	65570862	ID=Msa0218410-mRNA-1:exon:18295;Parent=Msa0218410-mRNA-1
chr2_1	exon	65570461	65570700	65570461	ID=Msa0218410-mRNA-1:exon:18294;Parent=Msa0218410-mRNA-1
chr2_1	exon	65569970	65570203	65569970	ID=Msa0218410-mRNA-1:exon:18293;Parent=Msa0218410-mRNA-1
chr2_1	exon	65564644	65564823	65564644	ID=Msa0218410-mRNA-1:exon:18292;Parent=Msa0218410-mRNA-1
chr2_1	exon	65564239	65564478	65564239	ID=Msa0218410-mRNA-1:exon:18291;Parent=Msa0218410-mRNA-1
chr2_1	exon	65563661	65563975	65563661	ID=Msa0218410-mRNA-1:exon:18290;Parent=Msa0218410-mRNA-1
chr2_1	CDS	65571128	65571133	65571128	ID=Msa0218410-mRNA-1:cds;Parent=Msa0218410-mRNA-1
chr2_1	CDS	65570862	65571041	65570862	ID=Msa0218410-mRNA-1:cds;Parent=Msa0218410-mRNA-1
chr2_1	CDS	65570461	65570700	65570461	ID=Msa0218410-mRNA-1:cds;Parent=Msa0218410-mRNA-1
chr2_1	CDS	65569970	65570203	65569970	ID=Msa0218410-mRNA-1:cds;Parent=Msa0218410-mRNA-1
chr2_1	CDS	65564644	65564823	65564644	ID=Msa0218410-mRNA-1:cds;Parent=Msa0218410-mRNA-1
chr2_1	CDS	65564239	65564478	65564239	ID=Msa0218410-mRNA-1:cds;Parent=Msa0218410-mRNA-1
chr2_1	CDS	65563661	65563975	65563661	ID=Msa0218410-mRNA-1:cds;Parent=Msa0218410-mRNA-1

Gene Sequence

>Msa0218410

ATGACGGATATGATGAGTGTACCTTGGCTTGTAAACTTGCTGGCAATCACTTCGTTTTTCACAACATGTGAAGCCCATCCAAACAAGTTCAGAAATGAGCGGAACAAGTTCTGCCTCGACTGCAATGAAAATCCATTATGTGAATTTTGTATAGAATCTCGTCACAAAGGTCATAGAGTAATACAGATAAGAAGATCATCATATAATGAGGTGGTTAAGACCATCGAAATTTACAAACATGTGGATATCCTCGGAATTCAAACCTACGTGATTAATAGCTCTAGTGTTGTATTCTTAAGCAAGAGAGCTCATGCCCAACCTAAGAGGTTCAACATTGGAAAAACCGGTCACAATAGGGACTCTTTTTGTAAGAGATGTGATAGAAATCTTGCAGACCCTACCTACTTTTGCTCTTTGGCATGCAAGTTTGAGTTTATCAAAAAGGATGGTGGCTTTTTCGTAAACCCAAGAGAGATGGAAGAAATGGAAAGATATCTAGATCAATCAATCAAGGCATCGCCTAGAAAAGAGAAGGCAAAAAAATGTTGCAAACAAAATCTGAAACGAAAATTAGAAGACGTAAGAATTGATGGGAAAGAGGAAGAGGAAGACGTAGAAAAAGAAAAGAAAGAAGGCGCCGATGACAAAGAAAGCATTCCGGATATGATGTGTGTACCTTGGCTTGTAAACTTGCTGGCAATCACTTCATTTTTCGCAACATGTGAAGCTCATCCAAACAAGTCGAAAAATGAGCGGAACAAGTTTTGTCTCGACTGCAATGAAAATCCATTATGTGAATCTTGTATAGAATCTCGTCACACAGATCATCGAGTAATCCAGATAAGGAGGTCATCATATAATGAAGTAGTGAAGACTAGTGAAATTTACAAACTTGTGGATATCCTCGGAATTCAAACCTATGTCATTAATAGCTCTAGTGTTGTATACTTAAACAAGAGAGCTCATGCCCAATCTAAGAGGTTCAACATTGGAAAAATGGGTCACAGTAGGGGCTCTTTTTGTAAGAGATGTGATATATATCTTATGGACTCTACTTATTTTTGCTCTTTGGCATGCAAGTTTGCGTTTATAAAAAAGGATGGAGGCTTTTTTGTAAACCCTAAAGAGATGGAAGAAATGGAAAGATATCTAGAGCAATCAATCAAGGCATCGCCTAAAAAAGAGAAGGCCAAAAAATATTGCAAACAAAATCTGAAACGAAAATTAGAAGACGCAAGAATTGATGGGAAAGAGGAAGAGGAAGACGTAGAAAAAGAAAGGGAAGAAGGCGCCGATGACAGAGAAAGCATTCTTCCAGTTAATCGTTCATTGGCTTCACTTCGCAAGCCAAATTCAAAAAGAAAAGGAATAGCTCATAGGGCACCTTTTTTTTAA

Protein sequence

>Msa0218410

MTDMMSVPWLVNLLAITSFFTTCEAHPNKFRNERNKFCLDCNENPLCEFCIESRHKGHRVIQIRRSSYNEVVKTIEIYKHVDILGIQTYVINSSSVVFLSKRAHAQPKRFNIGKTGHNRDSFCKRCDRNLADPTYFCSLACKFEFIKKDGGFFVNPREMEEMERYLDQSIKASPRKEKAKKCCKQNLKRKLEDVRIDGKEEEEDVEKEKKEGADDKESIPDMMCVPWLVNLLAITSFFATCEAHPNKSKNERNKFCLDCNENPLCESCIESRHTDHRVIQIRRSSYNEVVKTSEIYKLVDILGIQTYVINSSSVVYLNKRAHAQSKRFNIGKMGHSRGSFCKRCDIYLMDSTYFCSLACKFAFIKKDGGFFVNPKEMEEMERYLEQSIKASPKKEKAKKYCKQNLKRKLEDARIDGKEEEEDVEKEREEGADDRESILPVNRSLASLRKPNSKRKGIAHRAPFF*