AlfalfaGEDB | Msa0218620

M. sativa cultivar zhongmu-4 / Msa0218620

KEGG: -
GO: GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007154 GO:0007165 GO:0007602 GO:0008150 GO:0009314 GO:0009416 GO:0009581 GO:0009582 GO:0009583 GO:0009585 GO:0009605 GO:0009628 GO:0009639 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010017 GO:0010018 GO:0010218 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0042752 GO:0042753 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051606 GO:0051716 GO:0060255 GO:0065007 GO:0071214 GO:0071478 GO:0071482 GO:0071489 GO:0071490 GO:0080090 GO:0104004 GO:0140110 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0218620	XP_024632563.1	71.958	189	34	3	18	198	39	216	3.23e-76	263
Msa0218620	XP_024632563.1	73.020	404	29	5	116	448	301	695	0.0	567

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0218620	sp\|Q6NQJ7\|FRS4_ARATH	45.407	381	130	7	146	448	307	687	2.10e-96	308
Msa0218620	sp\|Q6NQJ7\|FRS4_ARATH	69.118	136	35	2	18	146	22	157	3.19e-53	193

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0218620	A0A396JDB2	71.958	189	34	3	18	198	39	216	1.54e-76	263
Msa0218620	A0A396JDB2	73.020	404	29	5	116	448	301	695	0.0	567

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TFs/TRs:

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Gene ID	Type	Classification
Msa0218620	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0218620	MtrunA17_Chr2g0329031	73.020	404	29	5	116	448	301	695	0.0	567
Msa0218620	MtrunA17_Chr2g0329031	71.958	189	34	3	18	198	39	216	2.97e-80	263
Msa0218620	MtrunA17_Chr1g0207551	38.235	340	133	5	146	411	350	686	1.59e-61	214
Msa0218620	MtrunA17_Chr1g0207551	43.151	146	60	5	18	145	59	199	7.89e-18	86.3
Msa0218620	MtrunA17_Chr3g0080351	37.821	312	118	4	158	393	396	707	1.58e-59	208
Msa0218620	MtrunA17_Chr3g0080351	52.632	95	23	2	18	90	101	195	5.64e-20	93.2
Msa0218620	MtrunA17_Chr3g0118611	29.749	279	128	3	148	358	265	543	7.27e-32	129
Msa0218620	MtrunA17_Chr5g0440451	29.286	280	128	4	148	358	368	646	1.78e-29	122
Msa0218620	MtrunA17_Chr5g0448761	25.974	308	145	5	159	383	398	705	8.68e-29	119
Msa0218620	MtrunA17_Chr5g0448511	24.150	294	145	4	159	374	170	463	7.25e-25	107

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0218620	AT1G76320.1	45.407	381	130	7	146	448	307	687	2.13e-97	308
Msa0218620	AT1G76320.1	69.118	136	35	2	18	146	22	157	3.24e-54	193
Msa0218620	AT1G76320.4	45.407	381	130	7	146	448	307	687	2.13e-97	308
Msa0218620	AT1G76320.4	69.118	136	35	2	18	146	22	157	3.24e-54	193
Msa0218620	AT1G76320.2	45.526	380	129	7	146	448	307	685	3.18e-97	308
Msa0218620	AT1G76320.2	69.118	136	35	2	18	146	22	157	3.65e-54	192
Msa0218620	AT1G76320.3	45.730	363	121	5	146	432	307	669	1.91e-96	304
Msa0218620	AT1G76320.3	69.118	136	35	2	18	146	22	157	1.26e-54	193
Msa0218620	AT4G15090.1	38.944	303	113	3	146	376	370	672	2.38e-65	224
Msa0218620	AT4G15090.1	41.611	149	61	6	18	145	76	219	3.95e-17	84.7

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Find 105 sgRNAs with CRISPR-Local

Find 386 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTTTCAGTTCGAGGTTT+TGG	0.254700	2_1:-65894384	Msa0218620:CDS
AACTCCCCGGAAAACATCAC+CGG	0.571503	2_1:-65895980	Msa0218620:five_prime_UTR
AAGAATTCGACTGCAGGTGG+TGG	0.578130	2_1:-65895382	Msa0218620:CDS
AAGAGGTTCAATCCAAGGTC+CGG	0.533736	2_1:-65894085	Msa0218620:CDS
AAGCCATGTAGTAACTTTCT+TGG	0.341888	2_1:+65894006	None:intergenic
AAGTTGTTTGAGACACTACA+AGG	0.562837	2_1:+65896026	None:intergenic
AATACCTGGACGGTTGTTTG+TGG	0.522553	2_1:+65895940	None:intergenic
ACAAATGTATATATCAATCA+TGG	0.501166	2_1:-65894772	Msa0218620:CDS
ACAAGATAAAGGGCGGAGTT+TGG	0.376984	2_1:-65895302	Msa0218620:intron
ACATGCACGATCATCATACA+AGG	0.650096	2_1:+65894673	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GAAAAAAGTAAATACCTGGA+CGG	+	chr2_1:65893798-65893817	None:intergenic	30.0%
AAGTCATTATTCCTCCATTT+AGG	-	chr2_1:65893899-65893918	Msa0218620:CDS	30.0%
AATCCATAATTGCACCTAAA+TGG	+	chr2_1:65893916-65893935	None:intergenic	30.0%
TCAAGACCCTAATAATGAAA+TGG	-	chr2_1:65893961-65893980	Msa0218620:CDS	30.0%
ATACGTTGAGTAATGATGTT+CGG	-	chr2_1:65894308-65894327	Msa0218620:intron	30.0%
ATGAATCGTCAACAAGATAA+AGG	-	chr2_1:65894412-65894431	Msa0218620:CDS	30.0%
TGAATCGTCAACAAGATAAA+GGG	-	chr2_1:65894413-65894432	Msa0218620:CDS	30.0%
TGAATTGTTTATGCATATGC+AGG	-	chr2_1:65894477-65894496	Msa0218620:CDS	30.0%
ATGCTCTTCATTCAAATCAA+TGG	+	chr2_1:65894527-65894546	None:intergenic	30.0%
TATATATCAATCATGGACAG+AGG	-	chr2_1:65894960-65894979	Msa0218620:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	65893648	65896099	65893648	ID=Msa0218620;Name=Msa0218620
chr2_1	mRNA	65893648	65896099	65893648	ID=Msa0218620-mRNA-1;Parent=Msa0218620;Name=Msa0218620-mRNA-1;_AED=0.16;_eAED=0.16;_QI=209\|0.25\|0.11\|1\|0.12\|0.22\|9\|0\|448
chr2_1	exon	65893648	65893698	65893648	ID=Msa0218620-mRNA-1:exon:18545;Parent=Msa0218620-mRNA-1
chr2_1	exon	65893782	65894002	65893782	ID=Msa0218620-mRNA-1:exon:18544;Parent=Msa0218620-mRNA-1
chr2_1	exon	65894021	65894233	65894021	ID=Msa0218620-mRNA-1:exon:18543;Parent=Msa0218620-mRNA-1
chr2_1	exon	65894384	65894586	65894384	ID=Msa0218620-mRNA-1:exon:18542;Parent=Msa0218620-mRNA-1
chr2_1	exon	65894632	65894852	65894632	ID=Msa0218620-mRNA-1:exon:18541;Parent=Msa0218620-mRNA-1
chr2_1	exon	65895303	65895500	65895303	ID=Msa0218620-mRNA-1:exon:18540;Parent=Msa0218620-mRNA-1
chr2_1	exon	65895519	65895706	65895519	ID=Msa0218620-mRNA-1:exon:18539;Parent=Msa0218620-mRNA-1
chr2_1	exon	65895724	65895826	65895724	ID=Msa0218620-mRNA-1:exon:18538;Parent=Msa0218620-mRNA-1
chr2_1	exon	65895945	65896099	65895945	ID=Msa0218620-mRNA-1:exon:18537;Parent=Msa0218620-mRNA-1
chr2_1	five_prime_UTR	65895945	65896099	65895945	ID=Msa0218620-mRNA-1:five_prime_utr;Parent=Msa0218620-mRNA-1
chr2_1	five_prime_UTR	65895773	65895826	65895773	ID=Msa0218620-mRNA-1:five_prime_utr;Parent=Msa0218620-mRNA-1
chr2_1	CDS	65895724	65895772	65895724	ID=Msa0218620-mRNA-1:cds;Parent=Msa0218620-mRNA-1
chr2_1	CDS	65895519	65895706	65895519	ID=Msa0218620-mRNA-1:cds;Parent=Msa0218620-mRNA-1
chr2_1	CDS	65895303	65895500	65895303	ID=Msa0218620-mRNA-1:cds;Parent=Msa0218620-mRNA-1
chr2_1	CDS	65894632	65894852	65894632	ID=Msa0218620-mRNA-1:cds;Parent=Msa0218620-mRNA-1
chr2_1	CDS	65894384	65894586	65894384	ID=Msa0218620-mRNA-1:cds;Parent=Msa0218620-mRNA-1
chr2_1	CDS	65894021	65894233	65894021	ID=Msa0218620-mRNA-1:cds;Parent=Msa0218620-mRNA-1
chr2_1	CDS	65893782	65894002	65893782	ID=Msa0218620-mRNA-1:cds;Parent=Msa0218620-mRNA-1
chr2_1	CDS	65893648	65893698	65893648	ID=Msa0218620-mRNA-1:cds;Parent=Msa0218620-mRNA-1

Gene Sequence

>Msa0218620

ATGAAATGGAATTTGAATCACATGAAGCTGCATATTCATTTTACAAAGAGGTTTGGTACTGCTAAGTTAAGTAGTCGCCGATCTAGGGCATCGAAAGAATTCATCGATGCGAAATTTTCATGTATTAGATATGGTAATAAACAACAATCGGATGACGCGATTAATCCGCGTCCTTCCCCGAAAATCGGTTGTAAAGCAAGTATGCATGTGAAGAGAAAACAGGATGGTAAATGGTATGAGCATAATCACGAGCTTTTACCTGCTCAAGCTCATTTTTTTCGGAGTCATAGGAATAATGATACGTTGAGTAATGATGTTCGGATGAGACGGAGGAAGAATTCGACTGCAGGTGGTGGTAAGTTGTTTAGCGCGTATCAGAATATTGATTGTTTGGAGAATTTGATGAATCGTCAACAAGATAAAGGGCGGAGTTTGATACCGAAACAGCTTGAGTTCTTAAGTACATGGCATGATAGCTTTATGGAAAAGTTCAACAAATGTATATATCAATCATGGACAGAGGAGCAATTTGAAAAGAGATGGTGGAAGCTGGTTGATAGGTTTAAACTTAGAGATGTTAAGTGGGTTCAAACCTTGTATGATGATCGTGCATGTTGGGTGCCAACGTTTATGAAGGATATTTCTTTGGCTGGCTTATATATCCAAGTTGATACTTCATTAAGACAATTTATAGATCAGTACCAATTGATTCTTGAAGATAGAGATGAAGAGGAAGCCAAAGCAAATTTTGATGCTTGGCATGAAACACCTGAATTAAAGTCACCTTCCCCTTTCGAAAAACAACTGCTGTTAGTTTATACTCATGAAATCTATCAAAAGTTTCAGTTCGAGGTTTTGGGTTACCTATGTAGACATGCAATTGTTGTTCTACAAATGTCTGGTGTTTTTAGCATCCCGCCAAAGTATATATTGAAAAGATGGACAAACGTCGCTTTAAGTAGGTATCCTATTGGTGAAAACTTGGAAGAGGTTCAATCCAAGGTCCGGAGATTAAATGATTTATGTAGACGAGCCATAATATTGGGTGAAGAAGGTTCTTTATCCCAAGAAAGTTCCTTAAGCGAGGCTCTAAAGCAATGTACAAATTTGAACAACTCTGTTGAGAATGCAGCCTTAGCTACCCATATTGGTTGCAATGTTGAAGAAGAGTATCAATCTATTATACCATCTAACGATAAGGTCTCTGATCCGAAAATGAATACGAGTAAGAAGGCTGCAAGGACTGGTGTAGCTGGTAGGAGTTCAGGAGGTGTTGAAGGAAGTAAGGGAAAGGTGCCTCAGCTTGGAGCAGTAAGTGGTAGTAACCATGGCTTTCAACAAATG

Protein sequence

>Msa0218620

MKWNLNHMKLHIHFTKRFGTAKLSSRRSRASKEFIDAKFSCIRYGNKQQSDDAINPRPSPKIGCKASMHVKRKQDGKWYEHNHELLPAQAHFFRSHRNNDTLSNDVRMRRRKNSTAGGGKLFSAYQNIDCLENLMNRQQDKGRSLIPKQLEFLSTWHDSFMEKFNKCIYQSWTEEQFEKRWWKLVDRFKLRDVKWVQTLYDDRACWVPTFMKDISLAGLYIQVDTSLRQFIDQYQLILEDRDEEEAKANFDAWHETPELKSPSPFEKQLLLVYTHEIYQKFQFEVLGYLCRHAIVVLQMSGVFSIPPKYILKRWTNVALSRYPIGENLEEVQSKVRRLNDLCRRAIILGEEGSLSQESSLSEALKQCTNLNNSVENAALATHIGCNVEEEYQSIIPSNDKVSDPKMNTSKKAARTGVAGRSSGGVEGSKGKVPQLGAVSGSNHGFQQM