AlfalfaGEDB | Msa0219740

M. sativa cultivar zhongmu-4 / Msa0219740

KEGG: -
GO: GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0009892 GO:0010468 GO:0010556 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0022603 GO:0031323 GO:0031326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0048519 GO:0048579 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048586 GO:0048831 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051171 GO:0051239 GO:0051241 GO:0051252 GO:0060255 GO:0060688 GO:0065007 GO:0080090 GO:0140110 GO:1900618 GO:1903506 GO:1905428 GO:2000026 GO:2000028 GO:2000032 GO:2000112 GO:2000241 GO:2000242 GO:2001141
PFAM: PF06203.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0219740	XP_039686690.1	94.457	433	24	0	1	433	1	433	0.0	855

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0219740	sp\|Q9LJ44\|COL12_ARATH	38.283	431	189	13	1	428	1	357	4.88e-81	257

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0219740	G7IKL4	92.841	433	23	1	1	433	97	521	0.0	833

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TFs/TRs:

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Gene ID	Type	Classification
Msa0219740	TR	Others

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0219740	MtrunA17_Chr2g0322941	93.000	200	14	0	1	200	40	239	1.42e-137	394
Msa0219740	MtrunA17_Chr2g0322951	95.238	147	7	0	287	433	1	147	1.57e-100	296
Msa0219740	MtrunA17_Chr5g0429341	34.014	441	242	13	1	429	1	404	6.19e-58	195
Msa0219740	MtrunA17_Chr3g0120711	33.110	447	239	14	1	429	1	405	1.95e-56	191
Msa0219740	MtrunA17_Chr7g0251781	27.753	454	227	19	1	422	1	385	5.18e-27	111
Msa0219740	MtrunA17_Chr1g0209071	44.231	104	57	1	1	104	1	103	5.50e-22	96.7
Msa0219740	MtrunA17_Chr7g0268691	45.000	100	54	1	5	103	20	119	5.46e-21	94.0
Msa0219740	MtrunA17_Chr3g0136701	43.902	82	46	0	4	85	5	86	1.43e-13	70.9
Msa0219740	MtrunA17_Chr4g0072871	37.805	82	51	0	4	85	21	102	4.95e-12	67.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0219740	AT3G21880.3	38.283	431	189	13	1	428	5	361	4.56e-82	257
Msa0219740	AT3G21880.2	38.283	431	189	13	1	428	5	361	4.56e-82	257
Msa0219740	AT3G21880.1	38.283	431	189	13	1	428	1	357	4.96e-82	257
Msa0219740	AT4G15250.1	35.945	434	168	11	1	430	1	328	1.81e-67	218
Msa0219740	AT5G48250.1	32.215	447	205	13	1	429	1	367	2.26e-55	188
Msa0219740	AT3G07650.3	32.671	453	194	15	1	429	1	366	1.44e-54	186
Msa0219740	AT3G07650.2	32.671	453	194	15	1	429	1	366	1.44e-54	186
Msa0219740	AT3G07650.4	32.671	453	194	15	1	429	1	366	1.44e-54	186
Msa0219740	AT3G07650.1	32.671	453	194	15	1	429	1	366	1.44e-54	186
Msa0219740	AT1G68190.1	44.361	133	66	2	1	129	10	138	5.06e-26	108

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Find 120 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAATGGCTTCGTTGGTT+TGG	0.319776	2_1:+67606708	Msa0219740:CDS
AAAGGTAGATTTGTTAAAGC+AGG	0.483112	2_1:+67609163	Msa0219740:CDS
AAATAATTGAGGAAGTATAA+AGG	0.451706	2_1:-67610498	None:intergenic
AAATACAACCACGGAGATGG+AGG	0.615014	2_1:+67607754	Msa0219740:CDS
AAATTCATCAAATGCTGCTT+GGG	0.439410	2_1:+67606609	Msa0219740:CDS
AACAGTATATGAAATAATTG+AGG	0.540401	2_1:-67610509	None:intergenic
AAGATGCATACAGTTGCTGC+AGG	0.570271	2_1:-67610469	None:intergenic
AAGCATAGACGAGCGGAATC+AGG	0.420641	2_1:-67606334	None:intergenic
AAGGATCTAATAATTCATGA+GGG	0.607523	2_1:+67607637	Msa0219740:CDS
ACAAAATACAACCACGGAGA+TGG	0.506317	2_1:+67607751	Msa0219740:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAGAACGAAATCAAAACCTT+CGG	-	chr2_1:67606874-67606893	None:intergenic	30.0%
TGAATATGTTTGCAAGTACA+TGG	-	chr2_1:67607180-67607199	None:intergenic	30.0%
GAATTGGAGATCAATTAAAG+GGG	-	chr2_1:67607347-67607366	None:intergenic	30.0%
CGAATTGGAGATCAATTAAA+GGG	-	chr2_1:67607348-67607367	None:intergenic	30.0%
TCGAATTGGAGATCAATTAA+AGG	-	chr2_1:67607349-67607368	None:intergenic	30.0%
TAGAGACTAATTCTTCGAAT+TGG	-	chr2_1:67607363-67607382	None:intergenic	30.0%
AGGATCTAATAATTCATGAG+GGG	+	chr2_1:67607638-67607657	Msa0219740:CDS	30.0%
GCAGTCAAATATTATTTCGT+CGG	-	chr2_1:67607719-67607738	None:intergenic	30.0%
AATTTCGACAACAATTTGGA+TGG	+	chr2_1:67608484-67608503	Msa0219740:intron	30.0%
GGCTAATTATGTTTAGTAGA+TGG	-	chr2_1:67608587-67608606	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	67606280	67610572	67606280	ID=Msa0219740;Name=Msa0219740
chr2_1	mRNA	67606280	67610572	67606280	ID=Msa0219740-mRNA-1;Parent=Msa0219740;Name=Msa0219740-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0\|0.5\|0.6\|1\|0.75\|0.8\|5\|473\|433
chr2_1	exon	67606280	67606876	67606280	ID=Msa0219740-mRNA-1:exon:19111;Parent=Msa0219740-mRNA-1
chr2_1	exon	67607616	67607849	67607616	ID=Msa0219740-mRNA-1:exon:19112;Parent=Msa0219740-mRNA-1
chr2_1	exon	67608075	67608421	67608075	ID=Msa0219740-mRNA-1:exon:19113;Parent=Msa0219740-mRNA-1
chr2_1	exon	67609102	67609276	67609102	ID=Msa0219740-mRNA-1:exon:19114;Parent=Msa0219740-mRNA-1
chr2_1	exon	67610151	67610572	67610151	ID=Msa0219740-mRNA-1:exon:19115;Parent=Msa0219740-mRNA-1
chr2_1	CDS	67606280	67606876	67606280	ID=Msa0219740-mRNA-1:cds;Parent=Msa0219740-mRNA-1
chr2_1	CDS	67607616	67607849	67607616	ID=Msa0219740-mRNA-1:cds;Parent=Msa0219740-mRNA-1
chr2_1	CDS	67608075	67608421	67608075	ID=Msa0219740-mRNA-1:cds;Parent=Msa0219740-mRNA-1
chr2_1	CDS	67609102	67609225	67609102	ID=Msa0219740-mRNA-1:cds;Parent=Msa0219740-mRNA-1
chr2_1	three_prime_UTR	67609226	67609276	67609226	ID=Msa0219740-mRNA-1:three_prime_utr;Parent=Msa0219740-mRNA-1
chr2_1	three_prime_UTR	67610151	67610572	67610151	ID=Msa0219740-mRNA-1:three_prime_utr;Parent=Msa0219740-mRNA-1

Gene Sequence

>Msa0219740

ATGGAAGCTCTATGTGAGTTTTGTGGGGTTGCAAGAGCTGTGGTTTACTGCAAGCCTGATTCCGCTCGTCTATGCTTGCATTGTGATGGGATTGTCCACTCTGCAAATTCTCTGTCTCGCAGGCATCCCCGCTCGCTCCTCTGCGATAAGTGTAATTTCGACTCAGCAATCGTGCGCTGCGTCGATCATAAATTGTCTTTCTGTCAAGTTTGTGATTGGAATACAAATGACTGTTTTGTATTGGGACATAAACATGTGCTACTCACTTTCTACACTGGTTGTCCCTCTTTGGCAGACTTATCTAAGATTTGGCCACATTTGGTTGATGCAAATTCATCAAATGCTGCTTGGGAATCACCTAGTACTAGTGCTTTGCCTAAAACTGAAATCAGTGACCGCCGCAATCAACATTTGGAGCAGAAACCCGAAAAGAATGGCTTCGTTGGTTTGGCGAATGACAAGTTGGGCGAAGGAGATCCATGTGTCAAATATGAACCTTGGATTGAAAATTCTCCTATTATTCCATCAAATTCAAACTGCACACAATACTTCAAAGATCAAGCATTTTTATTTAATCAAGATTCAAACCAACCGAAGCTTCAAGGATGCCATGATGTTAAGGATCTAATAATTCATGAGGGGACCGGTCTATGTGAAGGTTTCAATGTGGATGATGTTCAATTAAACTTTGAAAGTGCCGACGAAATAATATTTGACTGCTCTCAAACTGCCACAAAATACAACCACGGAGATGGAGGAATAGAATGTCTATTAATGGATAAAAACATTCCAGTCACAAAATGTAGCAGTCACATTGAGACTGCGGTAGAGGCTTCTTCATCAGTTCAACAAGATTGTATGATTTTTCCATCATCAGGGGCTGGTGGATCAACAAATTTGATGCAGGGCATCAACAATGGTGCAAATTGTGCACTTATGACTCCTAGTTGCAACAGAAGCATGCCTCTAGGGTTTCCTCAGAGTCAAACTCATTCAGGCATATCAATTCAATTACCTAACATAAATGGTGAAAGCAGTGTTACCGAACTTCTAGATTGTGCGTTACCTCCAGTGTTTCATCCTGGTGAATCGCATTGGGAATCAAATTTGGAGGGTGCTTGTCCTCAAGCAAGGGATAAAGCCAAAATGAGATACCAAGAGAAAAAGAAAACTCGAACGTTTGGTAAGCAAATAAGATATGCGTCTCGCAAAGCAAGGGCTGATACAAGAAAACGCGTGAAAGGTAGATTTGTTAAAGCAGGTGAAGCATATGACTATGATCCTCTTTTGAGCGACCACTAA

Protein sequence

>Msa0219740

MEALCEFCGVARAVVYCKPDSARLCLHCDGIVHSANSLSRRHPRSLLCDKCNFDSAIVRCVDHKLSFCQVCDWNTNDCFVLGHKHVLLTFYTGCPSLADLSKIWPHLVDANSSNAAWESPSTSALPKTEISDRRNQHLEQKPEKNGFVGLANDKLGEGDPCVKYEPWIENSPIIPSNSNCTQYFKDQAFLFNQDSNQPKLQGCHDVKDLIIHEGTGLCEGFNVDDVQLNFESADEIIFDCSQTATKYNHGDGGIECLLMDKNIPVTKCSSHIETAVEASSSVQQDCMIFPSSGAGGSTNLMQGINNGANCALMTPSCNRSMPLGFPQSQTHSGISIQLPNINGESSVTELLDCALPPVFHPGESHWESNLEGACPQARDKAKMRYQEKKKTRTFGKQIRYASRKARADTRKRVKGRFVKAGEAYDYDPLLSDH*