AlfalfaGEDB | Msa0220020

M. sativa cultivar zhongmu-4 / Msa0220020

KEGG: K17604
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0220020	XP_039685680.1	84.731	799	102	3	1	779	1	799	0.0	1389

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0220020	sp\|Q9SZL8\|FRS5_ARATH	27.769	641	383	14	32	665	70	637	1.19e-68	245

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0220020	D3YBB9	76.208	807	175	4	1	798	49	847	0.0	1279

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TFs/TRs:

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Gene ID	Type	Classification
Msa0220020	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0220020	MtrunA17_Chr3g0082121	31.838	691	383	17	41	727	49	655	1.88e-101	328
Msa0220020	MtrunA17_Chr3g0088371	82.609	184	29	1	618	798	1	184	4.31e-101	310
Msa0220020	MtrunA17_Chr2g0324011	30.551	635	365	12	36	665	8	571	5.80e-101	324
Msa0220020	MtrunA17_Chr1g0202341	29.606	635	366	17	34	662	50	609	2.59e-79	269
Msa0220020	MtrunA17_Chr8g0363841	28.776	629	375	13	40	665	22	580	1.88e-74	255
Msa0220020	MtrunA17_Chr1g0203721	26.026	780	452	20	1	739	16	711	4.93e-73	256
Msa0220020	MtrunA17_Chr5g0448761	25.035	707	451	14	31	728	56	692	2.19e-68	241
Msa0220020	MtrunA17_Chr2g0296781	27.910	670	397	20	39	699	1	593	7.06e-68	239
Msa0220020	MtrunA17_Chr6g0458391	26.984	630	357	15	41	662	231	765	5.49e-65	231
Msa0220020	MtrunA17_Chr6g0477261	29.222	527	315	14	180	699	7	482	2.85e-64	227

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0220020	AT4G38180.1	27.769	641	383	14	32	665	70	637	1.21e-69	245
Msa0220020	AT4G15090.1	24.827	721	448	16	50	753	66	709	1.76e-60	220
Msa0220020	AT4G15090.5	24.827	721	448	16	50	753	66	709	1.76e-60	220
Msa0220020	AT4G15090.3	24.827	721	448	16	50	753	66	709	1.76e-60	220
Msa0220020	AT4G15090.2	24.827	721	448	16	50	753	66	709	1.76e-60	220
Msa0220020	AT4G15090.4	24.827	721	448	16	50	753	66	709	1.76e-60	220
Msa0220020	AT2G27110.2	26.298	597	366	15	127	719	108	634	2.53e-53	199
Msa0220020	AT2G27110.1	26.298	597	366	15	127	719	108	634	2.53e-53	199
Msa0220020	AT2G27110.3	27.308	509	313	11	213	719	36	489	5.59e-53	196
Msa0220020	AT3G06250.2	24.961	633	352	16	34	661	189	703	2.47e-51	192

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Find 126 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGAAGTTCAAGGACAAC+TGG	0.438949	2_1:+68028380	Msa0220020:CDS
AAATGCTCTGGTTTATGTAA+TGG	0.504865	2_1:-68028330	None:intergenic
AACAAAGAAGGTTTCAAGAA+GGG	0.545537	2_1:+68028132	Msa0220020:CDS
AACAATAAATATCGGCCATT+AGG	0.388497	2_1:+68028732	Msa0220020:CDS
AACTCGTCAAAAGGAGATGG+TGG	0.603142	2_1:+68028524	Msa0220020:CDS
AAGAAAGACCAAATCCAATT+AGG	0.397771	2_1:+68030581	Msa0220020:CDS
AATCAGATTTCGACTACAAT+TGG	0.318540	2_1:+68029501	Msa0220020:CDS
AATCGAACAAGTGAATGGTC+TGG	0.540971	2_1:-68029704	None:intergenic
AATGAAGCTCTCTCGAAACC+AGG	0.565261	2_1:+68028582	Msa0220020:CDS
AATTCTTGATTGTTATGTAT+TGG	0.372244	2_1:+68030819	Msa0220020:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATATTAACTTCATCTGAGAG+TGG	+	chr2_1:68027880-68027899	Msa0220020:CDS	30.0%
CCATGATTCTTTATCACTAA+AGG	-	chr2_1:68027959-68027978	None:intergenic	30.0%
CCTTTAGTGATAAAGAATCA+TGG	+	chr2_1:68027956-68027975	Msa0220020:CDS	30.0%
TAAAGAATCATGGAAGTCTT+TGG	+	chr2_1:68027966-68027985	Msa0220020:CDS	30.0%
TAACAAAGAAGGTTTCAAGA+AGG	+	chr2_1:68028131-68028150	Msa0220020:CDS	30.0%
AACAAAGAAGGTTTCAAGAA+GGG	+	chr2_1:68028132-68028151	Msa0220020:CDS	30.0%
TAAAGAAAAACCAGAGGAAA+GGG	+	chr2_1:68028221-68028240	Msa0220020:CDS	30.0%
AGAACATCTTATGAAGTGAT+GGG	+	chr2_1:68028435-68028454	Msa0220020:CDS	30.0%
TTTAAGTCCGAAAATCGAAA+AGG	-	chr2_1:68028489-68028508	None:intergenic	30.0%
TGGATATCATAGTAAAAACC+TGG	-	chr2_1:68028603-68028622	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	68027826	68030860	68027826	ID=Msa0220020;Name=Msa0220020
chr2_1	mRNA	68027826	68030860	68027826	ID=Msa0220020-mRNA-1;Parent=Msa0220020;Name=Msa0220020-mRNA-1;_AED=0.00;_eAED=0.00;_QI=45\|1\|1\|1\|1\|1\|2\|152\|800
chr2_1	exon	68027826	68028753	68027826	ID=Msa0220020-mRNA-1:exon:19252;Parent=Msa0220020-mRNA-1
chr2_1	exon	68029189	68030860	68029189	ID=Msa0220020-mRNA-1:exon:19253;Parent=Msa0220020-mRNA-1
chr2_1	five_prime_UTR	68027826	68027870	68027826	ID=Msa0220020-mRNA-1:five_prime_utr;Parent=Msa0220020-mRNA-1
chr2_1	CDS	68027871	68028753	68027871	ID=Msa0220020-mRNA-1:cds;Parent=Msa0220020-mRNA-1
chr2_1	CDS	68029189	68030708	68029189	ID=Msa0220020-mRNA-1:cds;Parent=Msa0220020-mRNA-1
chr2_1	three_prime_UTR	68030709	68030860	68030709	ID=Msa0220020-mRNA-1:three_prime_utr;Parent=Msa0220020-mRNA-1

Gene Sequence

>Msa0220020

ATGGATTTGATATTAACTTCATCTGAGAGTGGACAATTGACGGTTGAAAATTCAGTTATTGATGCGGATAGTTCTCAATGTTACTCCTTTAGTGATAAAGAATCATGGAAGTCTTTGGTCTTTAAATCTATAAAAGAGATTGAAGATTTTTATTTTAATTATGCATATAACACTGGATTCTCTGTTAGGAGTATGTTGAAGTCCAAGAACAATTCACAAAATAAGAAATCGGATAAGGTACATTATGTGCGTTATGCTTGTAACAAAGAAGGTTTCAAGAAGGGAAGTCTGCTTAATCCTAAAAACAGGCCAACATGTGACAGTCCAGTTCTTGTTGAGTATGAGAAGGCTAAAGAAAAACCAGAGGAAAGGGTTGGTTGTAAAGCTGGAATATATTTGAAGTTGGATGAAGCGTTGAATGTCTATAAGATATATAAGTGGGAAGTTGATCACTGTCATCCATTACATAAACCAGAGCATTTGTGCTACTTGAGATCATTTCGAGAAGTAAATGAAGTTCAAGGACAACTGGCTTTAATAAATTCTAAAGCTGGAATGTCGATGAGAACATCTTATGAAGTGATGGGTCAAGGAGTTGGAGGCACGGAGAATCTTCCTTTTCGATTTTCGGACTTAAAGAACTATCTAATGACAACTCGTCAAAAGGAGATGGTGGCTGGTGAAGCAACTGTTATTCAAGAATTCTTTAGAAATGAAGCTCTCTCGAAACCAGGTTTTTACTATGATATCCAAGTTGATGCTGCAGAAGACATAGCCAGTATTTTTTGGGCAGATGGAATTATGCAACAAGATTATGCTCTATTTGGAGATGTTGTCAGTTTTGATACAACTTACCGAACAAACAATAAATATCGGCCATTAGCTGTCTTTCTTGGTTTTGACAATCATCGTAAGAGTGTGTTATTTGGTGCTGCGCTACTGTATGATGAGACATCAGCTACTTTTGATTGGTTGTTTACAACTTTTTTGAAGTGTATGTCCAATAAGAAACCTCAAACCATATACACAGACCAGGCTGGTGCGTTGTTGAAGTCAATTCCGAATGTTTTTCAAGGTGTTTTTCATGGTCTTTGCTCATGGCACATGGGAGAGAATGCAAAGAAAAATCTCGGCTCTCGTGCAAACAGTGCATTTTTCGATGAGTTAACTAATTTGGTTTCAAATGTCGAAGATGAATCAGATTTCGACTACAATTGGGATCAGATGATGAAAAACTGTTTTAATGGAAGGCCTACTTCGGACTTTAAGTGGCTTGTTCAAACTCATTCAAATCGTATGCATTGGTCTTCAGTTTGGGTGAGGTCACATTTTACAGCTGGTTTGAAAACGACTCAGTTAAGTGAGTCTTTCAATGCTTTTCTTCGTGGATTTTTGCAGCCAGACCATTCACTTGTTCGATTCTTTAGTCATTTCAACATTATGGTACAGAGAATGAGGGATAATCATGCTGAGTTGGACTTTAAGGCTGCAAACACTAGAACAAAAAATAATTATCCCAACAGTCAGCTTATGCGGTCCGTAGTAAACAAGTATACACCATCTTGCTTTTCATTTATTCACAGTCAGTATGACCTTTCCTTCAAATACTATTATGAGGAGGACACAACAAAAGGGTCTGAATTGAACAAGTTCTTCAAAGTTTTCACAATTGAGAAGGTTGATTATAATTACGATGATGTTGATAATGATAATGAGAGGGTTTCGAATGTTGATGTTTTGGATGCAGAATTTGAAGAAAACCTTTCCCCAAGGTTTGAAGATCATAATCGACTTGATGAAAGGGTTGTCACAGTCGACATTAGGAGTAAAAGTTTTACTTGTTCATGTCGTATGTTTGAGAACCGGGGTTTCTTATGTCGACATGTTTTCAAGATTTTGGAGGTCTTAGGTGGTCATGTGCAATATCATTTTTTGAAGACTATACCTGCACAATATGTTTTAAAGCGGTGGACTAGAGATGTGCGTCCGTCTGTCGATAAGCTGAAATCCACCATGAATGTTGGCACTAAAGACACCACTCAAGCACAACGATATCAACAAATTTGTGCTGTTACAGTTCAGCTTTCTACACGTGTTTGCGCAGCTGCGGAGGCATCTGAAATATTTCTCAACGGTGTTCTTGAGGCAGGGAAAAAGGCAGAGGAGTTTCTTATATCTAAAGGTATTCATACAGATCGATCATCCGTTACGCCTTCTAAGTCTTCAAAAGCAGTTGCTGTCTATGAACCATCTGTTGGGACTGCACCAAAGTTCAAAGAAAGACCAAATCCAATTAGGTCAAAGAAGCGACTTAAAAGTGATTATGAAAAAGCTAGAGCAAGACAGAAACTCCTTATAGCTCGGAAGAAGCAAAAGAAAGATGATGAGGCTTTAACAATTTAA

Protein sequence

>Msa0220020

MDLILTSSESGQLTVENSVIDADSSQCYSFSDKESWKSLVFKSIKEIEDFYFNYAYNTGFSVRSMLKSKNNSQNKKSDKVHYVRYACNKEGFKKGSLLNPKNRPTCDSPVLVEYEKAKEKPEERVGCKAGIYLKLDEALNVYKIYKWEVDHCHPLHKPEHLCYLRSFREVNEVQGQLALINSKAGMSMRTSYEVMGQGVGGTENLPFRFSDLKNYLMTTRQKEMVAGEATVIQEFFRNEALSKPGFYYDIQVDAAEDIASIFWADGIMQQDYALFGDVVSFDTTYRTNNKYRPLAVFLGFDNHRKSVLFGAALLYDETSATFDWLFTTFLKCMSNKKPQTIYTDQAGALLKSIPNVFQGVFHGLCSWHMGENAKKNLGSRANSAFFDELTNLVSNVEDESDFDYNWDQMMKNCFNGRPTSDFKWLVQTHSNRMHWSSVWVRSHFTAGLKTTQLSESFNAFLRGFLQPDHSLVRFFSHFNIMVQRMRDNHAELDFKAANTRTKNNYPNSQLMRSVVNKYTPSCFSFIHSQYDLSFKYYYEEDTTKGSELNKFFKVFTIEKVDYNYDDVDNDNERVSNVDVLDAEFEENLSPRFEDHNRLDERVVTVDIRSKSFTCSCRMFENRGFLCRHVFKILEVLGGHVQYHFLKTIPAQYVLKRWTRDVRPSVDKLKSTMNVGTKDTTQAQRYQQICAVTVQLSTRVCAAAEASEIFLNGVLEAGKKAEEFLISKGIHTDRSSVTPSKSSKAVAVYEPSVGTAPKFKERPNPIRSKKRLKSDYEKARARQKLLIARKKQKKDDEALTI*