AlfalfaGEDB | Msa0220150

M. sativa cultivar zhongmu-4 / Msa0220150

KEGG: -
GO: -
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0220150	RHN51853.1	32.184	174	69	2	9	182	9	133	3.69e-16	93.2
Msa0220150	RHN51853.1	37.423	163	89	1	34	183	222	384	4.65e-20	105
Msa0220150	RHN51853.1	43.682	277	142	2	203	466	225	500	1.27e-74	253

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0220150	A0A396HGY8	32.184	174	69	2	9	182	9	133	1.76e-16	93.2
Msa0220150	A0A396HGY8	37.423	163	89	1	34	183	222	384	2.22e-20	105
Msa0220150	A0A396HGY8	43.682	277	142	2	203	466	225	500	6.06e-75	253

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TFs/TRs:

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Gene ID	Type	Classification
Msa0220150	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0220150	MtrunA17_Chr6g0473661	43.682	277	142	2	203	466	225	500	1.17e-78	253
Msa0220150	MtrunA17_Chr6g0473661	37.423	163	89	1	34	183	222	384	4.27e-24	105
Msa0220150	MtrunA17_Chr6g0473661	32.184	174	69	2	9	182	9	133	3.40e-20	93.2
Msa0220150	MtrunA17_Chr6g0473651	49.640	139	69	1	212	350	216	353	1.47e-36	139
Msa0220150	MtrunA17_Chr6g0473651	44.531	128	71	0	56	183	226	353	2.04e-24	104
Msa0220150	MtrunA17_Chr6g0473651	31.214	173	108	3	279	443	23	192	3.18e-23	101
Msa0220150	MtrunA17_Chr6g0473651	47.887	71	37	0	112	182	22	92	8.05e-13	70.1
Msa0220150	MtrunA17_Chr6g0473921	36.957	138	87	0	328	465	1	138	8.28e-32	119
Msa0220150	MtrunA17_Chr1g0156191	37.500	136	84	1	294	429	1	135	1.55e-27	107
Msa0220150	MtrunA17_Chr1g0206471	29.767	215	139	2	207	410	38	251	1.38e-26	108

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 117 sgRNAs with CRISPR-Local

Find 251 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCACGACGGTCCAGTT+TGG	0.405460	2_1:-68336616	None:intergenic
AAACCCACCTAAGTCAGACA+TGG	0.685362	2_1:-68335301	None:intergenic
AAACCTATGGTCAGCGGCAG+TGG	0.455398	2_1:+68338574	Msa0220150:CDS
AAACTGGACCGTCGTGATTT+TGG	0.142859	2_1:+68336618	Msa0220150:CDS
AAATCTGTTGTTGATCGTCA+AGG	0.579813	2_1:-68336550	None:intergenic
AACCAGACTTTGCCGTGGTA+TGG	0.440028	2_1:+68338289	Msa0220150:intron
AACGCTTTGGATGCTGCACA+AGG	0.608033	2_1:+68335336	Msa0220150:CDS
AACGTATACCCCAAGTGAGA+TGG	0.579805	2_1:+68336162	Msa0220150:CDS
AATATGCCTTATGCTATGAT+TGG	0.515256	2_1:+68338412	Msa0220150:CDS
AATGAACCTCGGCAACGCAA+GGG	0.607154	2_1:-68338086	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACTCTTTATTATGGTCCTTT+AGG	+	chr2_1:68335716-68335735	Msa0220150:intron	30.0%
ATCAAACATAACGATATCAG+AGG	-	chr2_1:68335883-68335902	None:intergenic	30.0%
TATGTCCTCATCTATTAGAA+TGG	-	chr2_1:68336259-68336278	None:intergenic	30.0%
TAGTCTTTCAAAAAGCAGTT+AGG	-	chr2_1:68336348-68336367	None:intergenic	30.0%
TACTCCAATAATATCCAAAC+TGG	+	chr2_1:68336602-68336621	Msa0220150:CDS	30.0%
ATCAAATCTGCATTTGTAGA+CGG	-	chr2_1:68337078-68337097	None:intergenic	30.0%
ACAAATGCAGATTTGATTAG+AGG	+	chr2_1:68337081-68337100	Msa0220150:intron	30.0%
ACAACATATACTTCAGTTCA+GGG	-	chr2_1:68337175-68337194	None:intergenic	30.0%
AACAACATATACTTCAGTTC+AGG	-	chr2_1:68337176-68337195	None:intergenic	30.0%
ACGAATAACAATCAAATTGC+CGG	-	chr2_1:68337207-68337226	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	68335303	68338672	68335303	ID=Msa0220150;Name=Msa0220150
chr2_1	mRNA	68335303	68338672	68335303	ID=Msa0220150-mRNA-1;Parent=Msa0220150;Name=Msa0220150-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0\|0\|0\|0.5\|0.6\|0.66\|6\|0\|473
chr2_1	exon	68335303	68335375	68335303	ID=Msa0220150-mRNA-1:exon:19324;Parent=Msa0220150-mRNA-1
chr2_1	exon	68336140	68336225	68336140	ID=Msa0220150-mRNA-1:exon:19325;Parent=Msa0220150-mRNA-1
chr2_1	exon	68336330	68336747	68336330	ID=Msa0220150-mRNA-1:exon:19326;Parent=Msa0220150-mRNA-1
chr2_1	exon	68337258	68337337	68337258	ID=Msa0220150-mRNA-1:exon:19327;Parent=Msa0220150-mRNA-1
chr2_1	exon	68337809	68338195	68337809	ID=Msa0220150-mRNA-1:exon:19328;Parent=Msa0220150-mRNA-1
chr2_1	exon	68338295	68338672	68338295	ID=Msa0220150-mRNA-1:exon:19329;Parent=Msa0220150-mRNA-1
chr2_1	CDS	68335303	68335375	68335303	ID=Msa0220150-mRNA-1:cds;Parent=Msa0220150-mRNA-1
chr2_1	CDS	68336140	68336225	68336140	ID=Msa0220150-mRNA-1:cds;Parent=Msa0220150-mRNA-1
chr2_1	CDS	68336330	68336747	68336330	ID=Msa0220150-mRNA-1:cds;Parent=Msa0220150-mRNA-1
chr2_1	CDS	68337258	68337337	68337258	ID=Msa0220150-mRNA-1:cds;Parent=Msa0220150-mRNA-1
chr2_1	CDS	68337809	68338195	68337809	ID=Msa0220150-mRNA-1:cds;Parent=Msa0220150-mRNA-1
chr2_1	CDS	68338295	68338672	68338295	ID=Msa0220150-mRNA-1:cds;Parent=Msa0220150-mRNA-1

Gene Sequence

>Msa0220150

ATGTCTGACTTAGGTGGGTTTGTTTTAACTGGAAACGCTTTGGATGCTGCACAAGGAATGCTGTCATTGGCAGGTGGGGGTAACGACTTTCATCCAACGTATACCCCAAGTGAGATGGCAATGATGAGGACATACTTCCTTGAATTTGACACTATGTCTGGAATGGGTCGGATGCCTAACTGCTTTTTGAAAGACTATGGTGGTTATATTGAAGACTACGTTATGTTGCGAGATCCAATGAACAATGAGATTGAGGTTCGACTGTATCGCAGAGGCGACAAGCTTTATATGAGGAAAGGTTGGTATGGACTGAAGGACTTCTACAACATTGAGCTTGGTGCATGGATAGACTTGACATATGAAAGACCAAATCTTATGACCTTGACGATCAACAACAGATTTTTCGAGGAAATTTTGTATCCTGCTCACACTACTCCAATAATATCCAAACTGGACCGTCGTGATTTTGGGGGGAATTTGGATGATTTTTTTGAGACATTCACTATTGAATTGACTTACTCGGATGTTAACTCAGGATGTTTGGTATTCACTGTGGATTGCTTTATATTTCCGTTGTACTCTGCTAACGACCTCGATCCTGTGCGAAGGGACCTGTCTCGGTTGAGGACTTATCGTCTCGAGCTAGATAGCGACATTTTAAAGGCGAGGTTACCTTCATGTTTTGCAAAAGAAGTAGGCGTGCATATTGATGATCATGTTATGCTTCGTGATCCAAACAACAATATCATTGTTGTTGAAGTTCAGAAAAAGAATGGCAAGGTCTACTTCAGACAAGGCTGGTCTAGGGTGAAGGATATTTATAACATTGTAGATGGTGCATGGGTCTATCTGAAATATGCGGGGCCTAATCTACTACTCATGACCATAAGAAACAAGGCTGGATTGGAGATACCATATCCATTATCCGATATATCCCTTGCGTTGCCGAGGTTCATTACTTCATCAGGGGAGAGCCAGTTAGTGCATTTTTTCCGCACATCTGTCAAGATTTTGACTTCGTCTGATGTCCTCTCGGGCACTTTGACTTTGCCGTGGTATGGCTTCTGCCAGAAATCCTTGCCACAGAAACCTACCACACTTACGATTGTTGGTCCGAACGGCTGTACATGGAGATGTGATCTGACCTTCGCTGTTGCAAATAATATGCCTTATGCTATGATTGGTGGTGATTGGAACCTGATTTGCACATCGCAGAGGTTGGTTGCAGGCCATGCAGTTAAGTTCGCTGTTGTCCATGACAAAGATAACACAGTCCTTGGTTTCCGACATACCCCAATACACTGCACCCATGGATACTATGTGAAACCTATGGTCAGCGGCAGTGGTCGATATGTGTACCAGGTCAATCACTATCTCAAATATTGCAAGGCGAAGAGTATGCTGGAACAGATGTCGGGATGA

Protein sequence

>Msa0220150

MSDLGGFVLTGNALDAAQGMLSLAGGGNDFHPTYTPSEMAMMRTYFLEFDTMSGMGRMPNCFLKDYGGYIEDYVMLRDPMNNEIEVRLYRRGDKLYMRKGWYGLKDFYNIELGAWIDLTYERPNLMTLTINNRFFEEILYPAHTTPIISKLDRRDFGGNLDDFFETFTIELTYSDVNSGCLVFTVDCFIFPLYSANDLDPVRRDLSRLRTYRLELDSDILKARLPSCFAKEVGVHIDDHVMLRDPNNNIIVVEVQKKNGKVYFRQGWSRVKDIYNIVDGAWVYLKYAGPNLLLMTIRNKAGLEIPYPLSDISLALPRFITSSGESQLVHFFRTSVKILTSSDVLSGTLTLPWYGFCQKSLPQKPTTLTIVGPNGCTWRCDLTFAVANNMPYAMIGGDWNLICTSQRLVAGHAVKFAVVHDKDNTVLGFRHTPIHCTHGYYVKPMVSGSGRYVYQVNHYLKYCKAKSMLEQMSG*