AlfalfaGEDB | Msa0220380

M. sativa cultivar zhongmu-4 / Msa0220380

KEGG: K04733
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0007623 GO:0008150 GO:0008152 GO:0009506 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0030054 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048511 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564
PFAM: PF13855.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0220380	XP_013465046.1	83.051	177	4	1	496	646	600	776	3.62e-87	299
Msa0220380	XP_013465046.1	90.979	521	25	2	1	499	1	521	0.0	842

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0220380	sp\|Q6R2K3\|SRF3_ARATH	53.045	509	188	10	28	499	21	515	2.52e-128	400
Msa0220380	sp\|Q6R2K3\|SRF3_ARATH	65.761	184	33	2	490	643	585	768	6.50e-69	242

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0220380	A0A072VBT6	83.051	177	4	1	496	646	600	776	1.73e-87	299
Msa0220380	A0A072VBT6	90.979	521	25	2	1	499	1	521	0.0	842

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0220380	MtrunA17_Chr2g0323941	90.979	521	25	2	1	499	1	521	0.0	842
Msa0220380	MtrunA17_Chr2g0323941	83.051	177	4	1	496	646	600	776	3.33e-91	299
Msa0220380	MtrunA17_Chr4g0022431	53.345	583	177	5	10	499	3	583	1.92e-177	526
Msa0220380	MtrunA17_Chr4g0022431	65.657	198	37	3	484	650	646	843	2.20e-77	263
Msa0220380	MtrunA17_Chr2g0281501	41.715	513	222	15	13	499	4	465	3.89e-83	276
Msa0220380	MtrunA17_Chr2g0281501	60.843	166	39	1	496	635	545	710	1.19e-57	207
Msa0220380	MtrunA17_Chr2g0289161	35.532	470	245	9	33	499	36	450	2.81e-64	225
Msa0220380	MtrunA17_Chr2g0289161	54.286	175	51	4	496	642	530	703	2.62e-46	175
Msa0220380	MtrunA17_Chr4g0000081	41.975	243	135	3	17	254	1	242	5.89e-51	188
Msa0220380	MtrunA17_Chr4g0000081	48.276	174	56	4	496	642	516	682	2.44e-36	145

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0220380	AT4G03390.2	53.045	509	188	10	28	499	21	515	2.56e-129	400
Msa0220380	AT4G03390.2	65.761	184	33	2	490	643	585	768	6.61e-70	242
Msa0220380	AT4G03390.1	53.045	509	188	10	28	499	21	515	2.56e-129	400
Msa0220380	AT4G03390.1	65.761	184	33	2	490	643	585	768	6.61e-70	242
Msa0220380	AT2G20850.2	48.842	518	199	10	22	499	17	508	1.08e-124	384
Msa0220380	AT2G20850.2	69.811	53	16	0	496	548	588	640	1.95e-16	83.6
Msa0220380	AT2G20850.1	48.842	518	199	10	22	499	17	508	7.78e-124	385
Msa0220380	AT2G20850.1	64.286	182	39	1	493	648	585	766	3.22e-68	238
Msa0220380	AT3G14350.3	34.146	697	328	18	20	629	5	657	4.67e-94	305
Msa0220380	AT1G11130.1	38.148	540	255	13	20	499	7	527	7.17e-89	293

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Find 204 sgRNAs with CRISPR-Local

Find 492 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATACCAAAAGAGTGGTT+TGG	0.426095	2_1:-68556475	Msa0220380:CDS
AAACATCGAGTGTCCCAGAC+AGG	0.550055	2_1:+68558553	None:intergenic
AAAGACATCTGGTATTTCTC+CGG	0.512033	2_1:+68558884	None:intergenic
AAATTTCTGTGTATAACAGG+TGG	0.661356	2_1:+68554861	None:intergenic
AACAAATGGGTCAATTACCA+AGG	0.633661	2_1:+68553579	None:intergenic
AACAATCTTACCTTCCCATT+AGG	0.400230	2_1:+68555772	None:intergenic
AACCATGCCCCATGTTACCT+TGG	0.502983	2_1:-68553596	Msa0220380:three_prime_UTR
AACGGCTGTTGTAAGGCCAA+AGG	0.614964	2_1:-68556133	Msa0220380:CDS
AACGTGACAGGTAAACTTGA+TGG	0.507845	2_1:+68559195	None:intergenic
AACTAAAGGTTCTGTACGTC+AGG	0.434740	2_1:-68554766	Msa0220380:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGGTGCTTATATGGAATAAT+GGG	-	chr2_1:68554037-68554056	Msa0220380:intron	30.0%
TGTACCTAGTTTAGTTACTA+AGG	-	chr2_1:68554720-68554739	Msa0220380:intron	30.0%
TTAGTTACTAAGGCTAAGTT+TGG	-	chr2_1:68554730-68554749	Msa0220380:intron	30.0%
GAATAGATGATTCATGAATG+AGG	-	chr2_1:68555044-68555063	Msa0220380:intron	30.0%
ATAGGTAGATAACACATACA+GGG	+	chr2_1:68555795-68555814	None:intergenic	30.0%
ACATTAGTAACAAACTCACA+TGG	+	chr2_1:68555953-68555972	None:intergenic	30.0%
TTATTATCAAGAGACCTGAA+AGG	+	chr2_1:68556047-68556066	None:intergenic	30.0%
GTGAAAGATTAACTGAAATG+AGG	+	chr2_1:68556133-68556152	None:intergenic	30.0%
ATCTCATAACAATTTGAGTG+GGG	-	chr2_1:68556246-68556265	Msa0220380:intron	30.0%
CAAATCACCTTATCAAGAAA+TGG	+	chr2_1:68556322-68556341	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	68553449	68561532	68553449	ID=Msa0220380;Name=Msa0220380
chr2_1	mRNA	68553449	68561532	68553449	ID=Msa0220380-mRNA-1;Parent=Msa0220380;Name=Msa0220380-mRNA-1;_AED=0.18;_eAED=0.18;_QI=201\|0.75\|0.82\|0.94\|0.93\|0.88\|17\|433\|650
chr2_1	exon	68553449	68553980	68553449	ID=Msa0220380-mRNA-1:exon:19433;Parent=Msa0220380-mRNA-1
chr2_1	exon	68554344	68554422	68554344	ID=Msa0220380-mRNA-1:exon:19432;Parent=Msa0220380-mRNA-1
chr2_1	exon	68554509	68554645	68554509	ID=Msa0220380-mRNA-1:exon:19431;Parent=Msa0220380-mRNA-1
chr2_1	exon	68554767	68554905	68554767	ID=Msa0220380-mRNA-1:exon:19430;Parent=Msa0220380-mRNA-1
chr2_1	exon	68555547	68555569	68555547	ID=Msa0220380-mRNA-1:exon:19429;Parent=Msa0220380-mRNA-1
chr2_1	exon	68555783	68556162	68555783	ID=Msa0220380-mRNA-1:exon:19428;Parent=Msa0220380-mRNA-1
chr2_1	exon	68556348	68556700	68556348	ID=Msa0220380-mRNA-1:exon:19427;Parent=Msa0220380-mRNA-1
chr2_1	exon	68557619	68557684	68557619	ID=Msa0220380-mRNA-1:exon:19426;Parent=Msa0220380-mRNA-1
chr2_1	exon	68558528	68558593	68558528	ID=Msa0220380-mRNA-1:exon:19425;Parent=Msa0220380-mRNA-1
chr2_1	exon	68558670	68558741	68558670	ID=Msa0220380-mRNA-1:exon:19424;Parent=Msa0220380-mRNA-1
chr2_1	exon	68558858	68558929	68558858	ID=Msa0220380-mRNA-1:exon:19423;Parent=Msa0220380-mRNA-1
chr2_1	exon	68559028	68559099	68559028	ID=Msa0220380-mRNA-1:exon:19422;Parent=Msa0220380-mRNA-1
chr2_1	exon	68559186	68559251	68559186	ID=Msa0220380-mRNA-1:exon:19421;Parent=Msa0220380-mRNA-1
chr2_1	exon	68559377	68559448	68559377	ID=Msa0220380-mRNA-1:exon:19420;Parent=Msa0220380-mRNA-1
chr2_1	exon	68559530	68559659	68559530	ID=Msa0220380-mRNA-1:exon:19419;Parent=Msa0220380-mRNA-1
chr2_1	exon	68560653	68560807	68560653	ID=Msa0220380-mRNA-1:exon:19418;Parent=Msa0220380-mRNA-1
chr2_1	exon	68561360	68561532	68561360	ID=Msa0220380-mRNA-1:exon:19417;Parent=Msa0220380-mRNA-1
chr2_1	five_prime_UTR	68561360	68561532	68561360	ID=Msa0220380-mRNA-1:five_prime_utr;Parent=Msa0220380-mRNA-1
chr2_1	five_prime_UTR	68560780	68560807	68560780	ID=Msa0220380-mRNA-1:five_prime_utr;Parent=Msa0220380-mRNA-1
chr2_1	CDS	68560653	68560779	68560653	ID=Msa0220380-mRNA-1:cds;Parent=Msa0220380-mRNA-1
chr2_1	CDS	68559530	68559659	68559530	ID=Msa0220380-mRNA-1:cds;Parent=Msa0220380-mRNA-1
chr2_1	CDS	68559377	68559448	68559377	ID=Msa0220380-mRNA-1:cds;Parent=Msa0220380-mRNA-1
chr2_1	CDS	68559186	68559251	68559186	ID=Msa0220380-mRNA-1:cds;Parent=Msa0220380-mRNA-1
chr2_1	CDS	68559028	68559099	68559028	ID=Msa0220380-mRNA-1:cds;Parent=Msa0220380-mRNA-1
chr2_1	CDS	68558858	68558929	68558858	ID=Msa0220380-mRNA-1:cds;Parent=Msa0220380-mRNA-1
chr2_1	CDS	68558670	68558741	68558670	ID=Msa0220380-mRNA-1:cds;Parent=Msa0220380-mRNA-1
chr2_1	CDS	68558528	68558593	68558528	ID=Msa0220380-mRNA-1:cds;Parent=Msa0220380-mRNA-1
chr2_1	CDS	68557619	68557684	68557619	ID=Msa0220380-mRNA-1:cds;Parent=Msa0220380-mRNA-1
chr2_1	CDS	68556348	68556700	68556348	ID=Msa0220380-mRNA-1:cds;Parent=Msa0220380-mRNA-1
chr2_1	CDS	68555783	68556162	68555783	ID=Msa0220380-mRNA-1:cds;Parent=Msa0220380-mRNA-1
chr2_1	CDS	68555547	68555569	68555547	ID=Msa0220380-mRNA-1:cds;Parent=Msa0220380-mRNA-1
chr2_1	CDS	68554767	68554905	68554767	ID=Msa0220380-mRNA-1:cds;Parent=Msa0220380-mRNA-1
chr2_1	CDS	68554509	68554645	68554509	ID=Msa0220380-mRNA-1:cds;Parent=Msa0220380-mRNA-1
chr2_1	CDS	68554344	68554422	68554344	ID=Msa0220380-mRNA-1:cds;Parent=Msa0220380-mRNA-1
chr2_1	CDS	68553882	68553980	68553882	ID=Msa0220380-mRNA-1:cds;Parent=Msa0220380-mRNA-1
chr2_1	three_prime_UTR	68553449	68553881	68553449	ID=Msa0220380-mRNA-1:three_prime_utr;Parent=Msa0220380-mRNA-1

Gene Sequence

>Msa0220380

ATGTTAGAGATGAGATCTAGTTCACTGTACAAGAGAATGAAATTGAAAATTCATATACAAGTGATATTTGGATTGATGTTGATCTGTATAGTTCAGTTTTCACTTGCAGCTACTAATCCCACTGATGTTGCTGCAATTAATAGATTATATGCTGCATTGGGCAACCCTGTTCTTCCTGGGTGGGTTGCTAGTGCGGGAGACCCGTGCGGTGAAGCGTGGCAAGGCGTTCAATGTAATGATTCAGTCATACAAGAAATAACTCTGATTAGTGCAAATCTGGGTGGAGAATTGGGTGATAGCTTGGGGCCGTTTGTTTCTATCAAATCAATATCTCTTAGCAACAACCACATTGGAGGAAGTATTCCATCAAGTTTACCTGTCACGTTGCAATCCCTTTTTCTTTCAGGTAACCAGTTGACTGGAAGTATTCCAGTCTCTTTATCCACATTGACTGAGCTGACTGCCATGTCTCTTGATAATAATCTGTTAACCGGAGAAATACCAGATGTCTTTCAGTCTCTAACGCGATTGACCAATCTAGATTTATCTCATAACAATTTGAGTGGGGAATTGCCTCCATCTGTTGAGAATTTGTCCGCTGTGACCACCTTAAATTTACAGAACAATAGCCTGTCTGGGACACTCGATGTTTTGCAAGACCTTCCACTGACAGATTTGAATGTTGAGAACAACCAGTTTTCTGGACCAATACCTCCAAAGTTGTTAACCATTACCAACTTCAGAAAAGATGGAAACCAATTTGACCTCAGTAGTAATGTTACTATAGCACCATCTCGCCCACCTCATTCTCCGGTAACAGCATCTCCGGTGACAACATCACCAACAGGAACTGTAGTTTCTGGGACACCATCTTCTGGACGTGTGCCTACTAAACAGGCTGGTGGACCAACTGCATCAAAGGATTCAAGTTCCGGGAAATCCAAAAAAAATACCAAAAGAGTGGTTTGGATAACTATTTCTGGTGTATTGGGGTTTATTATCTTGGTACTGGCACTTCTTCTCTTTCTTCCAAGATGTAGCAAAAGGGAACGGGTCGGCAGAACTTCTAAGCAACATCAGATTGGAGCATATGGAGTACCAAAAACGGCTGTTGTAAGGCCAAAGGAGAATCATCAAGCAGAGAAAAATGTACAAAGAATGGAAGCATTGCCAAAGCTATTAAGTCATGACATAGATATGAGTGGACTAGATGTAGATTCAATGCCACCTCCACCTCCACCTCCTCCTCCACCTCCTGCTCCACCACTTGCTGCCGAGAAGGTGATCGTCGAGCCAACCACATTCCCCGGAGGGTCCAACATTAATCCTTCCAAAAGTAGTCTGGTTCCTCCAACATTTGCAAAATCGTTCACTATTGCAGCCCTTCAACAGTATACAAATAGCTTTTCTCAAGAAAATCTTATAGGATTAGGCATGTTGGGGAGTGTATATAGGGCAGAGCTTCCTAATGGGAAGATTTCCCTTGCTCAAAAAGTCTTGTACTTGCATGAGCAATGTCAGCCACCTGTTATACACAGAAATTTCAAGTCTGCTAACATTCTCCTTGATGAAGATCTATCTGTGCGTGTATCTGATTGTGGTTTGGCTCCACTAATAACTAAAGGTTCTGTACGTCAGCTTTCAGGACAGCTGCTAGCAGCTTACGGCTATGGGGCTCCAGAATTTGAGTCAGGAATTTACACCTATCAGAGTGATGTTTACAGCTTTGGAGTCGTTATGTTAGAACTTTTGACAGGCCGTCAGTCCTATGACAAAAAACGTCCTCGAGGTGAGCAGTTTCTTGTAAGATGGGCAATTACTCAACTTCATGACATTGATGCATTATCAAGGATGTCTGAGCCGGAATTTCGACCAGCGATGTCGGAGGTTGTCTTGTACTTGCTAAATATGATAAGGAGGGATTCTCAACAAAATGAATCAAATGAAAGATGA

Protein sequence

>Msa0220380

MLEMRSSSLYKRMKLKIHIQVIFGLMLICIVQFSLAATNPTDVAAINRLYAALGNPVLPGWVASAGDPCGEAWQGVQCNDSVIQEITLISANLGGELGDSLGPFVSIKSISLSNNHIGGSIPSSLPVTLQSLFLSGNQLTGSIPVSLSTLTELTAMSLDNNLLTGEIPDVFQSLTRLTNLDLSHNNLSGELPPSVENLSAVTTLNLQNNSLSGTLDVLQDLPLTDLNVENNQFSGPIPPKLLTITNFRKDGNQFDLSSNVTIAPSRPPHSPVTASPVTTSPTGTVVSGTPSSGRVPTKQAGGPTASKDSSSGKSKKNTKRVVWITISGVLGFIILVLALLLFLPRCSKRERVGRTSKQHQIGAYGVPKTAVVRPKENHQAEKNVQRMEALPKLLSHDIDMSGLDVDSMPPPPPPPPPPPAPPLAAEKVIVEPTTFPGGSNINPSKSSLVPPTFAKSFTIAALQQYTNSFSQENLIGLGMLGSVYRAELPNGKISLAQKVLYLHEQCQPPVIHRNFKSANILLDEDLSVRVSDCGLAPLITKGSVRQLSGQLLAAYGYGAPEFESGIYTYQSDVYSFGVVMLELLTGRQSYDKKRPRGEQFLVRWAITQLHDIDALSRMSEPEFRPAMSEVVLYLLNMIRRDSQQNESNER*