AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0220690

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0220690 MtrunA17_Chr2g0324381 84.500 200 15 1 199 382 1 200 4.60e-125 358
Msa0220690 MtrunA17_Chr2g0316511 57.068 191 61 4 208 379 1 189 5.22e-66 209
Msa0220690 MtrunA17_Chr2g0324351 51.613 248 52 5 10 189 237 484 6.87e-59 199
Msa0220690 MtrunA17_Chr3g0145031 48.958 192 74 5 208 377 1 190 3.00e-53 176
Msa0220690 MtrunA17_Chr3g0145041 49.412 170 64 4 229 381 8 172 8.71e-50 166
Msa0220690 MtrunA17_Chr2g0324361 53.125 96 27 2 232 311 14 107 1.76e-24 96.7
Msa0220690 MtrunA17_Chr5g0447451 43.119 109 53 3 264 363 20 128 2.83e-20 86.3
Msa0220690 MtrunA17_Chr4g0021121 58.750 80 25 2 115 189 435 511 1.57e-17 84.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0220690 AT4G22140.2 54.774 199 61 4 208 379 1 197 3.76e-64 204
Msa0220690 AT4G22140.1 54.774 199 61 4 208 379 1 197 5.92e-64 204
Msa0220690 AT4G22140.3 54.774 199 61 4 208 379 1 197 5.92e-64 204
Msa0220690 AT4G39100.1 47.753 178 73 3 219 378 14 189 3.10e-52 174
Msa0220690 AT4G04260.2 51.515 165 60 4 232 379 10 171 4.76e-43 149
Msa0220690 AT4G39100.2 48.993 149 58 2 219 349 14 162 7.49e-43 147
Msa0220690 AT4G04260.1 55.714 140 59 2 240 379 22 158 1.17e-42 147
Msa0220690 AT4G04260.3 47.857 140 53 4 208 327 1 140 4.61e-29 110

Find 109 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATGTTCACCTGATTTAA+AGG 0.193987 2_1:+69102841 Msa0220690:CDS
TCCTGAGAATTTGGTGAGTA+TGG 0.208742 2_1:+69100903 Msa0220690:CDS
GATTATGTGGTGCAGGATTC+TGG 0.223438 2_1:-69100837 None:intergenic
TTGAGCAAATTGAGAAAGTT+TGG 0.249482 2_1:-69100785 None:intergenic
AAAGGCATGCCCACGCATTT+AGG 0.253844 2_1:-69102776 None:intergenic
AGTACAGAATTAAAATTTAC+AGG 0.253996 2_1:-69100982 None:intergenic
TGAGGTTGTTGTGTGGTTAC+TGG 0.308496 2_1:-69100379 None:intergenic
TGTGACGCAGTTGGCGGATT+TGG 0.316080 2_1:-69100427 None:intergenic
GCGTTGCCATCCTGAGAATT+TGG 0.319880 2_1:+69100894 Msa0220690:CDS
TTCACCTGATTTAAAGGATT+AGG 0.322143 2_1:+69102847 Msa0220690:CDS
TCACCTGATTTAAAGGATTA+GGG 0.322613 2_1:+69102848 Msa0220690:CDS
ACGATTACTTTAGCAGGTTC+TGG 0.332292 2_1:+69102336 Msa0220690:CDS
ATTAACCTATCCGGGTTATA+AGG 0.334701 2_1:-69102530 None:intergenic
AGGATTAGGGACTTAGTAAC+TGG 0.354424 2_1:+69102861 Msa0220690:exon
TTATAACCCGGATAGGTTAA+TGG 0.355817 2_1:+69102532 Msa0220690:CDS
AATCAGAGGATGGAGGAAAC+TGG 0.360912 2_1:+69100955 Msa0220690:CDS
AACCCTTTGAGCAGCTAGTT+GGG 0.368728 2_1:+69102929 Msa0220690:three_prime_UTR
AACTTGTAGCTTGGCTTGTA+AGG 0.372323 2_1:-69100651 None:intergenic
GATTTCATTGCTTCATCAAA+AGG 0.383487 2_1:-69102794 None:intergenic
ACGCATAAGTTGCAGCTGGC+TGG 0.400394 2_1:-69100875 None:intergenic
ACTTACACTTCAACGCGTTC+AGG 0.403385 2_1:-69102386 None:intergenic
CACAGCACCATTAACCTATC+CGG 0.404617 2_1:-69102539 None:intergenic
TGAGCAAATTGAGAAAGTTT+GGG 0.409329 2_1:-69100784 None:intergenic
CGCATAAGTTGCAGCTGGCT+GGG 0.421179 2_1:-69100874 None:intergenic
CGCATTCTGTTTCTGAGAAA+GGG 0.423600 2_1:-69101538 None:intergenic
AGTCCCTAATCCTTTAAATC+AGG 0.432271 2_1:-69102851 None:intergenic
GCGCATTCTGTTTCTGAGAA+AGG 0.438042 2_1:-69101539 None:intergenic
TTGGTTTGTTCCTGCCAGTA+TGG 0.439578 2_1:-69102085 None:intergenic
AAATATTGTTGTGCGGTTGA+TGG 0.439764 2_1:-69100499 None:intergenic
TTAGGTACGCGCTCGGTTTG+CGG 0.441457 2_1:-69100733 None:intergenic
TATTGAGCTAGTCTGCATAC+TGG 0.449324 2_1:+69102962 Msa0220690:three_prime_UTR
CGACAACAAGGCAAGGGAAA+AGG 0.450024 2_1:+69101587 Msa0220690:CDS
AAAACATACGCGTCCTTCAA+TGG 0.453876 2_1:-69102192 None:intergenic
GGCACCAACACGGTCGTTAG+AGG 0.459119 2_1:+69101628 Msa0220690:CDS
CCCATACTCACCAAATTCTC+AGG 0.460854 2_1:-69100904 None:intergenic
GAACCCTTTGAGCAGCTAGT+TGG 0.462589 2_1:+69102928 Msa0220690:three_prime_UTR
TTCTGGAGGGAAGCACTTTG+AGG 0.474465 2_1:-69100820 None:intergenic
TTCCTGACCGTTGGATTATG+TGG 0.479887 2_1:-69100850 None:intergenic
GTGCTAACGATTACTTTAGC+AGG 0.480077 2_1:+69102330 Msa0220690:CDS
GTTACTGGATCTTGAGATGA+GGG 0.493647 2_1:-69100364 None:intergenic
AAACTTTCTCAATTTGCTCA+AGG 0.493671 2_1:+69100787 Msa0220690:CDS
CAGCAACCTACAGAACATAA+TGG 0.494473 2_1:+69101564 Msa0220690:CDS
GAACAGCATGCCGATGCAAA+AGG 0.498793 2_1:+69100696 Msa0220690:CDS
CGTCCTTCAATGGTTTCCTC+AGG 0.503827 2_1:-69102182 None:intergenic
ACCGTTGGATTATGTGGTGC+AGG 0.509994 2_1:-69100844 None:intergenic
TGGCTGGGATTCCTGACCGT+TGG 0.511267 2_1:-69100859 None:intergenic
AAAAGGAACAAATACACAAT+CGG 0.520774 2_1:+69101604 Msa0220690:CDS
GAAGGAAAATACTGAGTCAA+AGG 0.525283 2_1:-69100454 None:intergenic
CATAGTGCTGACACAATTGA+AGG 0.525786 2_1:+69102257 Msa0220690:CDS
ACCCCAACTAGCTGCTCAAA+GGG 0.527649 2_1:-69102931 None:intergenic
TTTGAGCAGCTAGTTGGGGT+TGG 0.532577 2_1:+69102934 Msa0220690:three_prime_UTR
GGAACTTGTGACGCAGTTGG+CGG 0.534842 2_1:-69100433 None:intergenic
TACATGAAGCTTGAAGTTGT+AGG 0.537934 2_1:+69102308 Msa0220690:CDS
CTTTCTCAATTTGCTCAAGG+TGG 0.538857 2_1:+69100790 Msa0220690:CDS
TTGTCGCCATTATGTTCTGT+AGG 0.543808 2_1:-69101570 None:intergenic
AACCCCAACTAGCTGCTCAA+AGG 0.545082 2_1:-69102932 None:intergenic
TGAGAGGTGAACTTGTAGCT+TGG 0.545890 2_1:-69100660 None:intergenic
CCTGAGAATTTGGTGAGTAT+GGG 0.549796 2_1:+69100904 Msa0220690:CDS
CGACCTGAGGAAACCATTGA+AGG 0.551081 2_1:+69102179 Msa0220690:CDS
GAGTGTAAGCATTTACTGAT+GGG 0.552591 2_1:-69100624 None:intergenic
CACCACATAATCCAACGGTC+AGG 0.554627 2_1:+69100848 Msa0220690:CDS
ATCAGAGGATGGAGGAAACT+GGG 0.556538 2_1:+69100956 Msa0220690:CDS
GGTAAGTATTAATCAGAGGA+TGG 0.560622 2_1:+69100945 Msa0220690:CDS
GGCAACGCATAAGTTGCAGC+TGG 0.563490 2_1:-69100879 None:intergenic
ACCCTTTGAGCAGCTAGTTG+GGG 0.569548 2_1:+69102930 Msa0220690:three_prime_UTR
GTAGCTTGGCTTGTAAGGTA+CGG 0.573169 2_1:-69100646 None:intergenic
GGTTACTGGATCTTGAGATG+AGG 0.574535 2_1:-69100365 None:intergenic
TTATGTTCTGTAGGTTGCTG+CGG 0.575653 2_1:-69101561 None:intergenic
GGCGGATTTGGTGAAGACGC+GGG 0.580512 2_1:-69100415 None:intergenic
CAGGTACCATCCTAAATGCG+TGG 0.581382 2_1:+69102766 Msa0220690:intron
GGAGTGTAAGCATTTACTGA+TGG 0.584343 2_1:-69100625 None:intergenic
ACAGCACCATTAACCTATCC+GGG 0.588194 2_1:-69102538 None:intergenic
AGATGCCTTATAACCCGGAT+AGG 0.594661 2_1:+69102525 Msa0220690:CDS
TACTGGATCTTGAGATGAGG+GGG 0.602448 2_1:-69100362 None:intergenic
GAAACAGTAGGCAACTTCCA+TGG 0.608176 2_1:-69102988 None:intergenic
AAAGGAACTTGTGACGCAGT+TGG 0.609957 2_1:-69100436 None:intergenic
CATGCTGTTCGGAAATGTGT+TGG 0.610516 2_1:-69100683 None:intergenic
GCATGCCCACGCATTTAGGA+TGG 0.615056 2_1:-69102772 None:intergenic
GGGAGTCAGAGGCGATCTTG+TGG 0.617629 2_1:+69100924 Msa0220690:CDS
TGGCGGATTTGGTGAAGACG+CGG 0.618742 2_1:-69100416 None:intergenic
ATAATGGCGACAACAAGGCA+AGG 0.623162 2_1:+69101580 Msa0220690:CDS
TTGGTGAGTATGGGAGTCAG+AGG 0.623193 2_1:+69100913 Msa0220690:CDS
ATGTGAGATGCCTTATAACC+CGG 0.623493 2_1:+69102520 Msa0220690:CDS
TACTCACCAAATTCTCAGGA+TGG 0.623583 2_1:-69100900 None:intergenic
TCATTCATTAGGTACGCGCT+CGG 0.625466 2_1:-69100740 None:intergenic
GCGGGCAGCATGTAATAATG+AGG 0.625949 2_1:-69100397 None:intergenic
TTCGGAAATGTGTTGGTGAG+AGG 0.626461 2_1:-69100676 None:intergenic
AAGTATTAATCAGAGGATGG+AGG 0.627703 2_1:+69100948 Msa0220690:CDS
AGTGTAAGCATTTACTGATG+GGG 0.631945 2_1:-69100623 None:intergenic
TTACTGGATCTTGAGATGAG+GGG 0.635937 2_1:-69100363 None:intergenic
AGGTACCATCCTAAATGCGT+GGG 0.636148 2_1:+69102767 Msa0220690:intron
AGAACATAATGGCGACAACA+AGG 0.636635 2_1:+69101575 Msa0220690:CDS
TGGAGGGAAGCACTTTGAGG+AGG 0.641258 2_1:-69100817 None:intergenic
TGTGTGGAAGTTGCAATCAA+AGG 0.645860 2_1:+69102558 Msa0220690:CDS
GTGTAAGCATTTACTGATGG+GGG 0.650672 2_1:-69100622 None:intergenic
TCCTGCACCACATAATCCAA+CGG 0.653869 2_1:+69100843 Msa0220690:CDS
TTGTGGTAAGTATTAATCAG+AGG 0.660821 2_1:+69100941 Msa0220690:CDS
TATGTGGTGCAGGATTCTGG+AGG 0.661370 2_1:-69100834 None:intergenic
ATAGTGCTGACACAATTGAA+GGG 0.668881 2_1:+69102258 Msa0220690:CDS
ATTACCTCTAACGACCGTGT+TGG 0.672565 2_1:-69101632 None:intergenic
AGTCTGCATACTGGATACCA+TGG 0.673066 2_1:+69102971 Msa0220690:three_prime_UTR
CACAATCGGAGGCACCAACA+CGG 0.673961 2_1:+69101618 Msa0220690:CDS
TAATGGCGACAACAAGGCAA+GGG 0.678546 2_1:+69101581 Msa0220690:CDS
AGGAACAAATACACAATCGG+AGG 0.686184 2_1:+69101607 Msa0220690:CDS
ATGTGGTGCAGGATTCTGGA+GGG 0.691226 2_1:-69100833 None:intergenic
GTAATAATGAGGTTGTTGTG+TGG 0.693156 2_1:-69100386 None:intergenic
ATACTGAAAATATTGTTGTG+CGG 0.696324 2_1:-69100506 None:intergenic
AAGAAGGTATTATCGACCTG+AGG 0.704026 2_1:+69102166 Msa0220690:intron
GATAGGTTAATGGTGCTGTG+TGG 0.736018 2_1:+69102542 Msa0220690:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGTTAAAGAAAATAAAAA+AGG + chr2_1:69101412-69101431 Msa0220690:intron 10.0%
!! GAAAAATTAATTAGGTTTAA+TGG - chr2_1:69101503-69101522 None:intergenic 15.0%
!!! AAACCTAATTAATTTTTCAT+AGG + chr2_1:69101505-69101524 Msa0220690:intron 15.0%
!! AGTACAGAATTAAAATTTAC+AGG - chr2_1:69100985-69101004 None:intergenic 20.0%
!! TAATGTTATATAGCATATCA+TGG + chr2_1:69101107-69101126 Msa0220690:intron 20.0%
!! TAATCCTCAAATATTACAAA+CGG - chr2_1:69101215-69101234 None:intergenic 20.0%
!! AAAAAAAAAAAACGTTATGC+TGG - chr2_1:69101289-69101308 None:intergenic 20.0%
!!! AAACACTGATATTTTTTTCT+AGG + chr2_1:69102411-69102430 Msa0220690:intron 20.0%
!!! AACACTGATATTTTTTTCTA+GGG + chr2_1:69102412-69102431 Msa0220690:intron 20.0%
!!! ATTTTACTTTTATGTTTCGA+AGG + chr2_1:69102489-69102508 Msa0220690:intron 20.0%
!! TGTATAATGAATTATTATGC+AGG + chr2_1:69102605-69102624 Msa0220690:intron 20.0%
! ATACTGAAAATATTGTTGTG+CGG - chr2_1:69100509-69100528 None:intergenic 25.0%
!!! ATTTTAATTCTGTACTTGAG+AGG + chr2_1:69100989-69101008 Msa0220690:CDS 25.0%
! ATGCTATATAACATTATTGC+CGG - chr2_1:69101103-69101122 None:intergenic 25.0%
! AATCCTCAAATATTACAAAC+GGG - chr2_1:69101214-69101233 None:intergenic 25.0%
! CGAATATATCAATTATTGTG+AGG + chr2_1:69101329-69101348 Msa0220690:intron 25.0%
! ATATCAATTATTGTGAGGAA+AGG + chr2_1:69101334-69101353 Msa0220690:intron 25.0%
!! TTCTTTTGAATAGATTCATG+TGG - chr2_1:69101367-69101386 None:intergenic 25.0%
! CTGCCTATGAAAAATTAATT+AGG - chr2_1:69101511-69101530 None:intergenic 25.0%
! AAAAGGAACAAATACACAAT+CGG + chr2_1:69101604-69101623 Msa0220690:CDS 25.0%
!! ATTTGAAGCAATTTGCTTAA+CGG - chr2_1:69101688-69101707 None:intergenic 25.0%
! AAGTAGATAGTTCAAAAACA+TGG + chr2_1:69101731-69101750 Msa0220690:intron 25.0%
!!! ACACTGATATTTTTTTCTAG+GGG + chr2_1:69102413-69102432 Msa0220690:intron 25.0%
!! ATCAAAGGTAAATTGGATTT+TGG + chr2_1:69102573-69102592 Msa0220690:intron 25.0%
!! TCAAAGGTAAATTGGATTTT+GGG + chr2_1:69102574-69102593 Msa0220690:intron 25.0%
! GAAATATTTCAATTTCTTGC+AGG + chr2_1:69102747-69102766 Msa0220690:intron 25.0%
! AAAATGTTCACCTGATTTAA+AGG + chr2_1:69102841-69102860 Msa0220690:CDS 25.0%
!!! CTCATTTTAGAATGCATTGA+GGG - chr2_1:69100601-69100620 None:intergenic 30.0%
!!! TCTCATTTTAGAATGCATTG+AGG - chr2_1:69100602-69100621 None:intergenic 30.0%
TGAGCAAATTGAGAAAGTTT+GGG - chr2_1:69100787-69100806 None:intergenic 30.0%
TTGAGCAAATTGAGAAAGTT+TGG - chr2_1:69100788-69100807 None:intergenic 30.0%
! AAACTTTCTCAATTTGCTCA+AGG + chr2_1:69100787-69100806 Msa0220690:CDS 30.0%
TTGTGGTAAGTATTAATCAG+AGG + chr2_1:69100941-69100960 Msa0220690:CDS 30.0%
TAGAATGTACAAAGTTCGAT+AGG + chr2_1:69101012-69101031 Msa0220690:intron 30.0%
ATCCTCAAATATTACAAACG+GGG - chr2_1:69101213-69101232 None:intergenic 30.0%
AAAAGGAATTACCTAATCGA+TGG + chr2_1:69101429-69101448 Msa0220690:intron 30.0%
! TTTGAAGCAATTTGCTTAAC+GGG - chr2_1:69101687-69101706 None:intergenic 30.0%
!!! GTTTTAAATTGCACTGATTG+TGG - chr2_1:69101977-69101996 None:intergenic 30.0%
AGGATAATATGAACAGAATG+AGG + chr2_1:69102135-69102154 Msa0220690:intron 30.0%
GGATAATATGAACAGAATGA+GGG + chr2_1:69102136-69102155 Msa0220690:intron 30.0%
AGTTGCAATCAAAGGTAAAT+TGG + chr2_1:69102566-69102585 Msa0220690:intron 30.0%
TTATTATGCAGGACCTTATT+TGG + chr2_1:69102616-69102635 Msa0220690:intron 30.0%
TGAATGTATGATGCCAAATA+AGG - chr2_1:69102632-69102651 None:intergenic 30.0%
AGATCTGTAAGGAAATAAAC+TGG - chr2_1:69102720-69102739 None:intergenic 30.0%
TTTCTGTCATAAGATCTGTA+AGG - chr2_1:69102731-69102750 None:intergenic 30.0%
GATTTCATTGCTTCATCAAA+AGG - chr2_1:69102797-69102816 None:intergenic 30.0%
TTCACCTGATTTAAAGGATT+AGG + chr2_1:69102847-69102866 Msa0220690:CDS 30.0%
TCACCTGATTTAAAGGATTA+GGG + chr2_1:69102848-69102867 Msa0220690:CDS 30.0%
!!! AAAGCTGTTTTCTTTTTCGA+AGG + chr2_1:69102896-69102915 Msa0220690:three_prime_UTR 30.0%
GTAATAATGAGGTTGTTGTG+TGG - chr2_1:69100389-69100408 None:intergenic 35.0%
GAAGGAAAATACTGAGTCAA+AGG - chr2_1:69100457-69100476 None:intergenic 35.0%
TATTGATACTGCAGATCAGA+AGG - chr2_1:69100475-69100494 None:intergenic 35.0%
AAATATTGTTGTGCGGTTGA+TGG - chr2_1:69100502-69100521 None:intergenic 35.0%
!!! ATTTTAGAATGCATTGAGGG+TGG - chr2_1:69100598-69100617 None:intergenic 35.0%
AGTGTAAGCATTTACTGATG+GGG - chr2_1:69100626-69100645 None:intergenic 35.0%
GAGTGTAAGCATTTACTGAT+GGG - chr2_1:69100627-69100646 None:intergenic 35.0%
! ACTGAATATGCCTTTTGCAT+CGG - chr2_1:69100709-69100728 None:intergenic 35.0%
GGTAAGTATTAATCAGAGGA+TGG + chr2_1:69100945-69100964 Msa0220690:CDS 35.0%
AAGTATTAATCAGAGGATGG+AGG + chr2_1:69100948-69100967 Msa0220690:CDS 35.0%
TCTGTTTGAATAACAGTGTC+CGG + chr2_1:69101081-69101100 Msa0220690:intron 35.0%
ATAGTAAAGTGCACATTGCT+AGG - chr2_1:69101152-69101171 None:intergenic 35.0%
TCCTCAAATATTACAAACGG+GGG - chr2_1:69101212-69101231 None:intergenic 35.0%
! CGTATGTTTTCATGGAGATA+AGG + chr2_1:69102205-69102224 Msa0220690:CDS 35.0%
!! ATAGTGCTGACACAATTGAA+GGG + chr2_1:69102258-69102277 Msa0220690:CDS 35.0%
TACATGAAGCTTGAAGTTGT+AGG + chr2_1:69102308-69102327 Msa0220690:CDS 35.0%
! TTCTGGTACAATGCTTTTAC+TGG + chr2_1:69102353-69102372 Msa0220690:CDS 35.0%
! TCTGGTACAATGCTTTTACT+GGG + chr2_1:69102354-69102373 Msa0220690:CDS 35.0%
ATTAACCTATCCGGGTTATA+AGG - chr2_1:69102533-69102552 None:intergenic 35.0%
TTATAACCCGGATAGGTTAA+TGG + chr2_1:69102532-69102551 Msa0220690:CDS 35.0%
TCTAAGAAGTAATGCTGTGT+TGG + chr2_1:69102685-69102704 Msa0220690:intron 35.0%
AGTCCCTAATCCTTTAAATC+AGG - chr2_1:69102854-69102873 None:intergenic 35.0%
TTACTGGATCTTGAGATGAG+GGG - chr2_1:69100366-69100385 None:intergenic 40.0%
GTTACTGGATCTTGAGATGA+GGG - chr2_1:69100367-69100386 None:intergenic 40.0%
GTGTAAGCATTTACTGATGG+GGG - chr2_1:69100625-69100644 None:intergenic 40.0%
GGAGTGTAAGCATTTACTGA+TGG - chr2_1:69100628-69100647 None:intergenic 40.0%
!! AACTTGTAGCTTGGCTTGTA+AGG - chr2_1:69100654-69100673 None:intergenic 40.0%
TTCTGCCACCTTCATTCATT+AGG - chr2_1:69100754-69100773 None:intergenic 40.0%
! CTTTCTCAATTTGCTCAAGG+TGG + chr2_1:69100790-69100809 Msa0220690:CDS 40.0%
TACTCACCAAATTCTCAGGA+TGG - chr2_1:69100903-69100922 None:intergenic 40.0%
! TCCTGAGAATTTGGTGAGTA+TGG + chr2_1:69100903-69100922 Msa0220690:CDS 40.0%
! CCTGAGAATTTGGTGAGTAT+GGG + chr2_1:69100904-69100923 Msa0220690:CDS 40.0%
AAAGTTCGATAGGTCTCCAT+AGG + chr2_1:69101022-69101041 Msa0220690:intron 40.0%
AAGTTCGATAGGTCTCCATA+GGG + chr2_1:69101023-69101042 Msa0220690:intron 40.0%
TCCCCCGTTTGTAATATTTG+AGG + chr2_1:69101208-69101227 Msa0220690:intron 40.0%
! TAAAATCGACGCCATCGATT+AGG - chr2_1:69101443-69101462 None:intergenic 40.0%
!! CGCATTCTGTTTCTGAGAAA+GGG - chr2_1:69101541-69101560 None:intergenic 40.0%
TTATGTTCTGTAGGTTGCTG+CGG - chr2_1:69101564-69101583 None:intergenic 40.0%
CAGCAACCTACAGAACATAA+TGG + chr2_1:69101564-69101583 Msa0220690:CDS 40.0%
TTGTCGCCATTATGTTCTGT+AGG - chr2_1:69101573-69101592 None:intergenic 40.0%
AGAACATAATGGCGACAACA+AGG + chr2_1:69101575-69101594 Msa0220690:CDS 40.0%
AGGAACAAATACACAATCGG+AGG + chr2_1:69101607-69101626 Msa0220690:CDS 40.0%
!! TAAGGAGAGAAGGGTTTTGA+GGG - chr2_1:69101658-69101677 None:intergenic 40.0%
! AAGCAATTTGCTTAACGGGA+AGG - chr2_1:69101683-69101702 None:intergenic 40.0%
! GCTTCAAATCGTTCCTTTTG+CGG + chr2_1:69101699-69101718 Msa0220690:intron 40.0%
ATTGCAGTGCAATGTTGAAG+AGG - chr2_1:69101811-69101830 None:intergenic 40.0%
TGGTGTGGTTACGAATAATG+TGG - chr2_1:69101864-69101883 None:intergenic 40.0%
! TCCACAGTTGTTAGGGTTTT+GGG - chr2_1:69101892-69101911 None:intergenic 40.0%
! AGTTTGCTCCACAGTTGTTA+GGG - chr2_1:69101899-69101918 None:intergenic 40.0%
CTATGTAAACGACGCTGATA+TGG - chr2_1:69102011-69102030 None:intergenic 40.0%
GAATGAGGGTCTATGTAAGA+AGG + chr2_1:69102150-69102169 Msa0220690:intron 40.0%
!! AAGAAGGTATTATCGACCTG+AGG + chr2_1:69102166-69102185 Msa0220690:intron 40.0%
AAAACATACGCGTCCTTCAA+TGG - chr2_1:69102195-69102214 None:intergenic 40.0%
!! CATAGTGCTGACACAATTGA+AGG + chr2_1:69102257-69102276 Msa0220690:CDS 40.0%
GTGCTAACGATTACTTTAGC+AGG + chr2_1:69102330-69102349 Msa0220690:CDS 40.0%
ACGATTACTTTAGCAGGTTC+TGG + chr2_1:69102336-69102355 Msa0220690:CDS 40.0%
! CTGGTACAATGCTTTTACTG+GGG + chr2_1:69102355-69102374 Msa0220690:CDS 40.0%
ATGTGAGATGCCTTATAACC+CGG + chr2_1:69102520-69102539 Msa0220690:CDS 40.0%
TGTGTGGAAGTTGCAATCAA+AGG + chr2_1:69102558-69102577 Msa0220690:CDS 40.0%
AGGATTAGGGACTTAGTAAC+TGG + chr2_1:69102861-69102880 Msa0220690:exon 40.0%
! TATTGAGCTAGTCTGCATAC+TGG + chr2_1:69102962-69102981 Msa0220690:three_prime_UTR 40.0%
TACTGGATCTTGAGATGAGG+GGG - chr2_1:69100365-69100384 None:intergenic 45.0%
GGTTACTGGATCTTGAGATG+AGG - chr2_1:69100368-69100387 None:intergenic 45.0%
TGAGGTTGTTGTGTGGTTAC+TGG - chr2_1:69100382-69100401 None:intergenic 45.0%
AAAGGAACTTGTGACGCAGT+TGG - chr2_1:69100439-69100458 None:intergenic 45.0%
! CATTGTTGTTGCTGACGATG+CGG - chr2_1:69100538-69100557 None:intergenic 45.0%
!! GTAGCTTGGCTTGTAAGGTA+CGG - chr2_1:69100649-69100668 None:intergenic 45.0%
TGAGAGGTGAACTTGTAGCT+TGG - chr2_1:69100663-69100682 None:intergenic 45.0%
! TTCGGAAATGTGTTGGTGAG+AGG - chr2_1:69100679-69100698 None:intergenic 45.0%
CATGCTGTTCGGAAATGTGT+TGG - chr2_1:69100686-69100705 None:intergenic 45.0%
!! TTTTGCATCGGCATGCTGTT+CGG - chr2_1:69100697-69100716 None:intergenic 45.0%
TCATTCATTAGGTACGCGCT+CGG - chr2_1:69100743-69100762 None:intergenic 45.0%
AGCGCGTACCTAATGAATGA+AGG + chr2_1:69100743-69100762 Msa0220690:intron 45.0%
GCGTACCTAATGAATGAAGG+TGG + chr2_1:69100746-69100765 Msa0220690:intron 45.0%
GATTATGTGGTGCAGGATTC+TGG - chr2_1:69100840-69100859 None:intergenic 45.0%
TCCTGCACCACATAATCCAA+CGG + chr2_1:69100843-69100862 Msa0220690:CDS 45.0%
TTCCTGACCGTTGGATTATG+TGG - chr2_1:69100853-69100872 None:intergenic 45.0%
CCCATACTCACCAAATTCTC+AGG - chr2_1:69100907-69100926 None:intergenic 45.0%
AATCAGAGGATGGAGGAAAC+TGG + chr2_1:69100955-69100974 Msa0220690:CDS 45.0%
ATCAGAGGATGGAGGAAACT+GGG + chr2_1:69100956-69100975 Msa0220690:CDS 45.0%
AGTTCGATAGGTCTCCATAG+GGG + chr2_1:69101024-69101043 Msa0220690:intron 45.0%
!! GCGCATTCTGTTTCTGAGAA+AGG - chr2_1:69101542-69101561 None:intergenic 45.0%
ATAATGGCGACAACAAGGCA+AGG + chr2_1:69101580-69101599 Msa0220690:CDS 45.0%
TAATGGCGACAACAAGGCAA+GGG + chr2_1:69101581-69101600 Msa0220690:CDS 45.0%
ATTACCTCTAACGACCGTGT+TGG - chr2_1:69101635-69101654 None:intergenic 45.0%
! GTAAGGAGAGAAGGGTTTTG+AGG - chr2_1:69101659-69101678 None:intergenic 45.0%
! TTGCTTAACGGGAAGGTGTA+AGG - chr2_1:69101676-69101695 None:intergenic 45.0%
ACTTCACTCGATTCCGCAAA+AGG - chr2_1:69101715-69101734 None:intergenic 45.0%
CTGCCATAACAGCGAAATTG+CGG - chr2_1:69101788-69101807 None:intergenic 45.0%
!!! TGTTAGGGTTTTGGGGTAGT+TGG - chr2_1:69101884-69101903 None:intergenic 45.0%
! CCACAGTTGTTAGGGTTTTG+GGG - chr2_1:69101891-69101910 None:intergenic 45.0%
! CTCCACAGTTGTTAGGGTTT+TGG - chr2_1:69101893-69101912 None:intergenic 45.0%
! CAGTTTGCTCCACAGTTGTT+AGG - chr2_1:69101900-69101919 None:intergenic 45.0%
! TGTGTTTTGCTACGCCATAC+TGG + chr2_1:69102071-69102090 Msa0220690:CDS 45.0%
!! TTGGTTTGTTCCTGCCAGTA+TGG - chr2_1:69102088-69102107 None:intergenic 45.0%
!! TTTTCCACACGGGCTATGAT+TGG - chr2_1:69102107-69102126 None:intergenic 45.0%
! CTGCTCAATCTTTTCCACAC+GGG - chr2_1:69102117-69102136 None:intergenic 45.0%
! CCTGCTCAATCTTTTCCACA+CGG - chr2_1:69102118-69102137 None:intergenic 45.0%
! GAAGGACGCGTATGTTTTCA+TGG + chr2_1:69102197-69102216 Msa0220690:CDS 45.0%
ACTTACACTTCAACGCGTTC+AGG - chr2_1:69102389-69102408 None:intergenic 45.0%
AGATGCCTTATAACCCGGAT+AGG + chr2_1:69102525-69102544 Msa0220690:CDS 45.0%
ACAGCACCATTAACCTATCC+GGG - chr2_1:69102541-69102560 None:intergenic 45.0%
CACAGCACCATTAACCTATC+CGG - chr2_1:69102542-69102561 None:intergenic 45.0%
!! GATAGGTTAATGGTGCTGTG+TGG + chr2_1:69102542-69102561 Msa0220690:CDS 45.0%
AGGTACCATCCTAAATGCGT+GGG + chr2_1:69102767-69102786 Msa0220690:intron 45.0%
AACCCTTTGAGCAGCTAGTT+GGG + chr2_1:69102929-69102948 Msa0220690:three_prime_UTR 45.0%
AGTCTGCATACTGGATACCA+TGG + chr2_1:69102971-69102990 Msa0220690:three_prime_UTR 45.0%
GCGGGCAGCATGTAATAATG+AGG - chr2_1:69100400-69100419 None:intergenic 50.0%
CGCATCGTCAGCAACAACAA+TGG + chr2_1:69100536-69100555 Msa0220690:intron 50.0%
GAACAGCATGCCGATGCAAA+AGG + chr2_1:69100696-69100715 Msa0220690:CDS 50.0%
!! TTCTGGAGGGAAGCACTTTG+AGG - chr2_1:69100823-69100842 None:intergenic 50.0%
ATGTGGTGCAGGATTCTGGA+GGG - chr2_1:69100836-69100855 None:intergenic 50.0%
TATGTGGTGCAGGATTCTGG+AGG - chr2_1:69100837-69100856 None:intergenic 50.0%
ACCGTTGGATTATGTGGTGC+AGG - chr2_1:69100847-69100866 None:intergenic 50.0%
CACCACATAATCCAACGGTC+AGG + chr2_1:69100848-69100867 Msa0220690:CDS 50.0%
GCGTTGCCATCCTGAGAATT+TGG + chr2_1:69100894-69100913 Msa0220690:CDS 50.0%
TTGGTGAGTATGGGAGTCAG+AGG + chr2_1:69100913-69100932 Msa0220690:CDS 50.0%
CGACAACAAGGCAAGGGAAA+AGG + chr2_1:69101587-69101606 Msa0220690:CDS 50.0%
! GGGAAGGTGTAAGGAGAGAA+GGG - chr2_1:69101667-69101686 None:intergenic 50.0%
CGAAATTGCGGCTGCAATTG+CGG - chr2_1:69101776-69101795 None:intergenic 50.0%
CAGCCGCAATTTCGCTGTTA+TGG + chr2_1:69101782-69101801 Msa0220690:intron 50.0%
CCCCAAAACCCTAACAACTG+TGG + chr2_1:69101888-69101907 Msa0220690:intron 50.0%
GACACACATTGCGGTCATTG+TGG - chr2_1:69101947-69101966 None:intergenic 50.0%
! TTTTGCTACGCCATACTGGC+AGG + chr2_1:69102075-69102094 Msa0220690:CDS 50.0%
CAAACCAATCATAGCCCGTG+TGG + chr2_1:69102100-69102119 Msa0220690:intron 50.0%
CCGTGTGGAAAAGATTGAGC+AGG + chr2_1:69102115-69102134 Msa0220690:intron 50.0%
CGACCTGAGGAAACCATTGA+AGG + chr2_1:69102179-69102198 Msa0220690:CDS 50.0%
CGTCCTTCAATGGTTTCCTC+AGG - chr2_1:69102185-69102204 None:intergenic 50.0%
CAGGTACCATCCTAAATGCG+TGG + chr2_1:69102766-69102785 Msa0220690:intron 50.0%
! AAAGGCATGCCCACGCATTT+AGG - chr2_1:69102779-69102798 None:intergenic 50.0%
GAACCCTTTGAGCAGCTAGT+TGG + chr2_1:69102928-69102947 Msa0220690:three_prime_UTR 50.0%
ACCCTTTGAGCAGCTAGTTG+GGG + chr2_1:69102930-69102949 Msa0220690:three_prime_UTR 50.0%
ACCCCAACTAGCTGCTCAAA+GGG - chr2_1:69102934-69102953 None:intergenic 50.0%
AACCCCAACTAGCTGCTCAA+AGG - chr2_1:69102935-69102954 None:intergenic 50.0%
TTTGAGCAGCTAGTTGGGGT+TGG + chr2_1:69102934-69102953 Msa0220690:three_prime_UTR 50.0%
!! TGGCGGATTTGGTGAAGACG+CGG - chr2_1:69100419-69100438 None:intergenic 55.0%
!! TGTGACGCAGTTGGCGGATT+TGG - chr2_1:69100430-69100449 None:intergenic 55.0%
GGAACTTGTGACGCAGTTGG+CGG - chr2_1:69100436-69100455 None:intergenic 55.0%
ACCTGCTGAGGTGACTGTTG+AGG - chr2_1:69100562-69100581 None:intergenic 55.0%
TGCAGAGATTGCACCTGCTG+AGG - chr2_1:69100574-69100593 None:intergenic 55.0%
! TTAGGTACGCGCTCGGTTTG+CGG - chr2_1:69100736-69100755 None:intergenic 55.0%
!! TGGAGGGAAGCACTTTGAGG+AGG - chr2_1:69100820-69100839 None:intergenic 55.0%
CGCATAAGTTGCAGCTGGCT+GGG - chr2_1:69100877-69100896 None:intergenic 55.0%
ACGCATAAGTTGCAGCTGGC+TGG - chr2_1:69100878-69100897 None:intergenic 55.0%
GGCAACGCATAAGTTGCAGC+TGG - chr2_1:69100882-69100901 None:intergenic 55.0%
CGATAGGTCTCCATAGGGGA+TGG + chr2_1:69101028-69101047 Msa0220690:intron 55.0%
GTCTCCATAGGGGATGGTCA+GGG + chr2_1:69101034-69101053 Msa0220690:intron 55.0%
TTCACCCTGACCATCCCCTA+TGG - chr2_1:69101041-69101060 None:intergenic 55.0%
!! CACAATCGGAGGCACCAACA+CGG + chr2_1:69101618-69101637 Msa0220690:CDS 55.0%
! CGGGAAGGTGTAAGGAGAGA+AGG - chr2_1:69101668-69101687 None:intergenic 55.0%
!!! GGGTTTTGGGGTAGTTGGTG+TGG - chr2_1:69101879-69101898 None:intergenic 55.0%
GGATGCTGCGACACACATTG+CGG - chr2_1:69101956-69101975 None:intergenic 55.0%
GCATGCCCACGCATTTAGGA+TGG - chr2_1:69102775-69102794 None:intergenic 55.0%
!! GGCGGATTTGGTGAAGACGC+GGG - chr2_1:69100418-69100437 None:intergenic 60.0%
GCCTCAACAGTCACCTCAGC+AGG + chr2_1:69100558-69100577 Msa0220690:intron 60.0%
TGGCTGGGATTCCTGACCGT+TGG - chr2_1:69100862-69100881 None:intergenic 60.0%
GGGAGTCAGAGGCGATCTTG+TGG + chr2_1:69100924-69100943 Msa0220690:CDS 60.0%
GGTCTCCATAGGGGATGGTC+AGG + chr2_1:69101033-69101052 Msa0220690:intron 60.0%
GGCACCAACACGGTCGTTAG+AGG + chr2_1:69101628-69101647 Msa0220690:CDS 60.0%
TGCGGCTGCAATTGCGGTTG+CGG - chr2_1:69101770-69101789 None:intergenic 60.0%
GCGGCTGCAATTGCGGTTGC+GGG - chr2_1:69101769-69101788 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 69100358 69103001 69100358 ID=Msa0220690;Name=Msa0220690
chr2_1 mRNA 69100358 69103001 69100358 ID=Msa0220690-mRNA-1;Parent=Msa0220690;Name=Msa0220690-mRNA-1;_AED=0.40;_eAED=0.42;_QI=0|0.33|0.2|1|0.33|0.2|10|133|382
chr2_1 exon 69100358 69100464 69100358 ID=Msa0220690-mRNA-1:exon:19564;Parent=Msa0220690-mRNA-1
chr2_1 exon 69100487 69100541 69100487 ID=Msa0220690-mRNA-1:exon:19565;Parent=Msa0220690-mRNA-1
chr2_1 exon 69100580 69100747 69100580 ID=Msa0220690-mRNA-1:exon:19566;Parent=Msa0220690-mRNA-1
chr2_1 exon 69100772 69101010 69100772 ID=Msa0220690-mRNA-1:exon:19567;Parent=Msa0220690-mRNA-1
chr2_1 exon 69101531 69101649 69101531 ID=Msa0220690-mRNA-1:exon:19568;Parent=Msa0220690-mRNA-1
chr2_1 exon 69102063 69102116 69102063 ID=Msa0220690-mRNA-1:exon:19569;Parent=Msa0220690-mRNA-1
chr2_1 exon 69102157 69102163 69102157 ID=Msa0220690-mRNA-1:exon:19570;Parent=Msa0220690-mRNA-1
chr2_1 exon 69102172 69102402 69102172 ID=Msa0220690-mRNA-1:exon:19571;Parent=Msa0220690-mRNA-1
chr2_1 exon 69102511 69102579 69102511 ID=Msa0220690-mRNA-1:exon:19572;Parent=Msa0220690-mRNA-1
chr2_1 exon 69102769 69103001 69102769 ID=Msa0220690-mRNA-1:exon:19573;Parent=Msa0220690-mRNA-1
chr2_1 CDS 69100358 69100464 69100358 ID=Msa0220690-mRNA-1:cds;Parent=Msa0220690-mRNA-1
chr2_1 CDS 69100487 69100541 69100487 ID=Msa0220690-mRNA-1:cds;Parent=Msa0220690-mRNA-1
chr2_1 CDS 69100580 69100747 69100580 ID=Msa0220690-mRNA-1:cds;Parent=Msa0220690-mRNA-1
chr2_1 CDS 69100772 69101010 69100772 ID=Msa0220690-mRNA-1:cds;Parent=Msa0220690-mRNA-1
chr2_1 CDS 69101531 69101649 69101531 ID=Msa0220690-mRNA-1:cds;Parent=Msa0220690-mRNA-1
chr2_1 CDS 69102063 69102116 69102063 ID=Msa0220690-mRNA-1:cds;Parent=Msa0220690-mRNA-1
chr2_1 CDS 69102157 69102163 69102157 ID=Msa0220690-mRNA-1:cds;Parent=Msa0220690-mRNA-1
chr2_1 CDS 69102172 69102402 69102172 ID=Msa0220690-mRNA-1:cds;Parent=Msa0220690-mRNA-1
chr2_1 CDS 69102511 69102579 69102511 ID=Msa0220690-mRNA-1:cds;Parent=Msa0220690-mRNA-1
chr2_1 CDS 69102769 69102868 69102769 ID=Msa0220690-mRNA-1:cds;Parent=Msa0220690-mRNA-1
chr2_1 three_prime_UTR 69102869 69103001 69102869 ID=Msa0220690-mRNA-1:three_prime_utr;Parent=Msa0220690-mRNA-1
Gene Sequence

>Msa0220690

ATGACCCCCTCATCTCAAGATCCAGTAACCACACAACAACCTCATTATTACATGCTGCCCGCGTCTTCACCAAATCCGCCAACTGCGTCACAAGTTCCTTTGACTCATATCAATATCATCCATCAACCGCACAACAATATTTTCAGTATCAGCAGCCGCATCGTGCAATCTCTGCAACCACCCTCAATGCATTCTAAAATGAGACCCCCATCAGTAAATGCTTACACTCCGTACCTTACAAGCCAAGCTACAAGTTCACCTCTCACCAACACATTTCCGAACAGCATGCCGATGCAAAAGGCATATTCAGTGACGCCGCAAACCGAGCGCAAAAATTACTCTCCCAAACTTTCTCAATTTGCTCAAGGTGGATATCCTCCTCAAAGTGCTTCCCTCCAGAATCCTGCACCACATAATCCAACGGTCAGGAATCCCAGCCAGCTGCAACTTATGCGTTGCCATCCTGAGAATTTGGTGAGTATGGGAGTCAGAGGCGATCTTGTGGTAAGTATTAATCAGAGGATGGAGGAAACTGGGCAGCCTGTAAATTTTAATTCTGTACTTGAGAGCAGCTCTCCCTTTCTCAGAAACAGAATGCGCCGCAGCAACCTACAGAACATAATGGCGACAACAAGGCAAGGGAAAAGGAACAAATACACAATCGGAGGCACCAACACGGTCGTTAGAGCTAAAGACTGTGTTTTGCTACGCCATACTGGCAGGAACAAACCAATCATAGCCCGGTCTATGTATTATCGACCTGAGGAAACCATTGAAGGACGCGTATGTTTTCATGGAGATAAGGAGCTGTTTTTGTCTAATCATTATGATGTTCATAGTGCTGACACAATTGAAGGGAAGTGTTTTGTGCACACTTTGAAGAACTACATGAAGCTTGAAGTTGTAGGTGCTAACGATTACTTTAGCAGGTTCTGGTACAATGCTTTTACTGGGGCTATGACACCTGAACGCGTTGAAGTGTTGTGCAAATGTGAGATGCCTTATAACCCGGATAGGTTAATGGTGCTGTGTGGAAGTTGCAATCAAAGGTACCATCCTAAATGCGTGGGCATGCCTTTTGATGAAGCAATGAAATCAGAAGATTTTAGATTTGTTTGTTCAAAATGTTCACCTGATTTAAAGGATTAG
Protein sequence

>Msa0220690

MTPSSQDPVTTQQPHYYMLPASSPNPPTASQVPLTHINIIHQPHNNIFSISSRIVQSLQPPSMHSKMRPPSVNAYTPYLTSQATSSPLTNTFPNSMPMQKAYSVTPQTERKNYSPKLSQFAQGGYPPQSASLQNPAPHNPTVRNPSQLQLMRCHPENLVSMGVRGDLVVSINQRMEETGQPVNFNSVLESSSPFLRNRMRRSNLQNIMATTRQGKRNKYTIGGTNTVVRAKDCVLLRHTGRNKPIIARSMYYRPEETIEGRVCFHGDKELFLSNHYDVHSADTIEGKCFVHTLKNYMKLEVVGANDYFSRFWYNAFTGAMTPERVEVLCKCEMPYNPDRLMVLCGSCNQRYHPKCVGMPFDEAMKSEDFRFVCSKCSPDLKD*