AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0221170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0221170 MtrunA17_Chr2g0325521 98.381 247 4 0 1 247 1 247 7.22e-180 493
Msa0221170 MtrunA17_Chr4g0041051 71.875 256 63 3 1 247 1 256 6.45e-121 344
Msa0221170 MtrunA17_Chr4g0045901 44.672 244 124 6 2 235 13 255 4.52e-51 167
Msa0221170 MtrunA17_Chr7g0255961 47.253 182 86 4 54 234 70 242 3.74e-42 144
Msa0221170 MtrunA17_Chr1g0198801 40.594 202 109 3 45 242 144 338 2.16e-34 126
Msa0221170 MtrunA17_Chr3g0113721 33.333 201 115 5 46 234 26 219 7.00e-31 114
Msa0221170 MtrunA17_Chr5g0412371 38.462 143 74 3 54 188 40 176 4.12e-26 101
Msa0221170 MtrunA17_Chr5g0427361 40.606 165 72 2 49 188 27 190 4.73e-25 99.4
Msa0221170 MtrunA17_Chr2g0283714 51.250 80 36 1 91 170 16 92 3.47e-20 83.6
Msa0221170 MtrunA17_Chr5g0411341 40.244 82 49 0 28 109 459 540 3.21e-12 65.9
Msa0221170 MtrunA17_Chr8g0366751 39.506 81 49 0 29 109 429 509 8.35e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0221170 AT1G68810.1 41.327 196 107 5 45 236 160 351 2.27e-33 124
Msa0221170 AT3G25710.1 46.622 148 70 2 45 188 118 260 1.01e-31 119
Msa0221170 AT3G56770.1 38.857 175 98 4 51 223 37 204 1.60e-30 113
Msa0221170 AT2G40200.1 39.634 164 89 3 61 219 65 223 1.99e-29 111
Msa0221170 AT2G41130.1 40.571 175 96 3 51 223 59 227 4.30e-27 105
Msa0221170 AT4G38070.1 36.154 130 76 2 59 188 92 214 1.29e-17 80.1
Msa0221170 AT4G17880.1 40.789 76 45 0 39 114 393 468 2.24e-11 63.5
Msa0221170 AT5G46760.1 44.156 77 42 1 39 114 391 467 2.29e-11 63.5
Msa0221170 AT1G32640.1 36.634 101 61 2 33 130 421 521 3.64e-11 63.2

Find 68 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATCTTCTTCACTGGTTT+TGG 0.180837 2_1:-69767114 Msa0221170:CDS
CACTTCATCTTATGATAATA+AGG 0.194458 2_1:-69765797 Msa0221170:CDS
ATTGAATGATGAAAAGAAAA+AGG 0.226203 2_1:+69767353 None:intergenic
AATGATGCTCTAATTGAATA+AGG 0.233216 2_1:+69765873 None:intergenic
TGTTGGGGTTGGAGTGTATT+TGG 0.233217 2_1:+69767394 None:intergenic
CTTATCTCATCACTGTCTTT+TGG 0.234136 2_1:+69765930 None:intergenic
ATATTCAGTTCTTCATCTTT+AGG 0.245592 2_1:-69765582 Msa0221170:CDS
CCAAGAGGGTGTTCAGAATA+TGG 0.263756 2_1:+69767229 None:intergenic
TCACAAGAAGGTGGATTAAA+TGG 0.279597 2_1:-69765900 Msa0221170:CDS
TTTGCTGTTGATGCTTCTTC+TGG 0.299468 2_1:+69767489 None:intergenic
GGCTGTTATATTTGTGTGTT+TGG 0.304280 2_1:+69767310 None:intergenic
TTCACATGTAATGTGAATCT+TGG 0.305383 2_1:-69765527 Msa0221170:three_prime_UTR
TGTTGTTAATTTGTCATGTT+GGG 0.312268 2_1:+69767378 None:intergenic
ACAATGAATGAACTCATTCT+AGG 0.319427 2_1:-69765423 Msa0221170:three_prime_UTR
TTTCAAGGTGTAAATGGAAA+TGG 0.359014 2_1:-69767143 Msa0221170:CDS
TGTGGAGGCAGCTGTGAAAT+TGG 0.360925 2_1:-69767072 Msa0221170:CDS
TGTTATCCCAGGTGCTAATA+AGG 0.365921 2_1:-69766940 Msa0221170:intron
TGTAATAACTTCAGCTAGTA+AGG 0.369694 2_1:+69766007 None:intergenic
AAGGGCAGAGATTGCAACTT+TGG 0.372459 2_1:-69765778 Msa0221170:CDS
GTAAAGATTACTTCTTATAG+TGG 0.377802 2_1:+69767511 None:intergenic
TATGATGATGATAGTGAGTT+TGG 0.389810 2_1:+69767437 None:intergenic
TTGTTGTTAATTTGTCATGT+TGG 0.399163 2_1:+69767377 None:intergenic
GAGTATCGACAGTTTCTTGC+TGG 0.401116 2_1:-69765693 Msa0221170:CDS
AGGGCAGAGATTGCAACTTT+GGG 0.424505 2_1:-69765777 Msa0221170:CDS
TCTTATATCAGACAATAAAC+CGG 0.424693 2_1:+69765833 None:intergenic
CCATATTCTGAACACCCTCT+TGG 0.428144 2_1:-69767229 Msa0221170:five_prime_UTR
GGTGTAAATGGAAATGGATA+TGG 0.430429 2_1:-69767137 Msa0221170:CDS
TTAATTTGTCATGTTGGGGT+TGG 0.442961 2_1:+69767383 None:intergenic
ATGATGCTCTAATTGAATAA+GGG 0.448376 2_1:+69765874 None:intergenic
ATCTTGGGTTGTACATTTGT+TGG 0.451003 2_1:-69765511 Msa0221170:three_prime_UTR
ATATGGTTCATCTTCTTCAC+TGG 0.451383 2_1:-69767120 Msa0221170:CDS
TCTTGGGTTGTACATTTGTT+GGG 0.456987 2_1:-69765510 Msa0221170:three_prime_UTR
CATATTCTGAACACCCTCTT+GGG 0.457908 2_1:-69767228 Msa0221170:five_prime_UTR
AGCACTACTCTCTCTGCCTC+TGG 0.479098 2_1:-69767466 Msa0221170:five_prime_UTR
TAAAGATTACTTCTTATAGT+GGG 0.485015 2_1:+69767512 None:intergenic
ACTTCATCTTATGATAATAA+GGG 0.489015 2_1:-69765796 Msa0221170:CDS
TCGACAGTTTCTTGCTGGAT+CGG 0.489881 2_1:-69765688 Msa0221170:CDS
AAAGCTTCAATACTTCTCTC+AGG 0.498631 2_1:+69767032 None:intergenic
CTTATATCAGACAATAAACC+GGG 0.504302 2_1:+69765834 None:intergenic
TTATATTTGTGTGTTTGGAA+TGG 0.508497 2_1:+69767315 None:intergenic
TCACATGTAATGTGAATCTT+GGG 0.510126 2_1:-69765526 Msa0221170:three_prime_UTR
GGAATAGAAGTTATGTCAGC+AGG 0.512851 2_1:+69765373 None:intergenic
GAACTAACCTTATTAGCACC+TGG 0.521161 2_1:+69766933 None:intergenic
AAGACAACGTTGGAAGAAGA+AGG 0.529141 2_1:+69767274 None:intergenic
AGCAAGAAACTGTCGATACT+CGG 0.542573 2_1:+69765695 None:intergenic
GTTGTTAATTTGTCATGTTG+GGG 0.557500 2_1:+69767379 None:intergenic
TTATGTTGTGAATACAAACC+CGG 0.569012 2_1:-69765852 Msa0221170:CDS
GTGAGTTTGGTGAGATCCAG+AGG 0.570542 2_1:+69767450 None:intergenic
AACTAACCTTATTAGCACCT+GGG 0.573545 2_1:+69766934 None:intergenic
AATGCAGCACAAGCAAGTGA+AGG 0.583141 2_1:-69765963 Msa0221170:CDS
AAAGATTACTTCTTATAGTG+GGG 0.583347 2_1:+69767513 None:intergenic
GCTTCAAGTAGAATGAATGA+TGG 0.589941 2_1:-69767170 Msa0221170:CDS
GCAGAGATTGCAACTTTGGG+AGG 0.592208 2_1:-69765774 Msa0221170:CDS
GAAGAAGGATGAGGAGACAA+AGG 0.595640 2_1:+69767289 None:intergenic
ATGCAGCACAAGCAAGTGAA+GGG 0.603381 2_1:-69765962 Msa0221170:CDS
ATCATAGTGAAGCAGAGAGG+AGG 0.603979 2_1:-69767004 Msa0221170:CDS
TCTTAGAAACTAGAAAGAGA+AGG 0.613686 2_1:-69765614 Msa0221170:CDS
TAGAAACTAGAAAGAGAAGG+AGG 0.614835 2_1:-69765611 Msa0221170:CDS
AAGATTACTTCTTATAGTGG+GGG 0.618050 2_1:+69767514 None:intergenic
ATAAGAGTTGAATCACAAGA+AGG 0.621854 2_1:-69765912 Msa0221170:CDS
ACACTTCGTAGTGTTATCCC+AGG 0.622939 2_1:-69766951 Msa0221170:CDS
AGAGTTGAATCACAAGAAGG+TGG 0.624818 2_1:-69765909 Msa0221170:CDS
GACACAAAGAAAGACAACGT+TGG 0.650206 2_1:+69767264 None:intergenic
AAACAACACAGTTCCCAAGA+GGG 0.661371 2_1:+69767215 None:intergenic
ACGTTGGAAGAAGAAGGATG+AGG 0.668662 2_1:+69767280 None:intergenic
AAAACAACACAGTTCCCAAG+AGG 0.688172 2_1:+69767214 None:intergenic
GCTGTGAAATTGGAACGCAA+AGG 0.704659 2_1:-69767062 Msa0221170:CDS
AGAATCATAGTGAAGCAGAG+AGG 0.708784 2_1:-69767007 Msa0221170:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GGAATAGAATAATTTAATTT+TGG - chr2_1:69766667-69766686 Msa0221170:intron 15.0%
!!! GGAATAGAATAATTTAATTT+TGG - chr2_1:69766667-69766686 Msa0221170:intron 15.0%
!! TAAAGATTACTTCTTATAGT+GGG + chr2_1:69765375-69765394 None:intergenic 20.0%
!! TTGAATGATGAAAAGAAAAA+GGG + chr2_1:69765533-69765552 None:intergenic 20.0%
!! ATTGAATGATGAAAAGAAAA+AGG + chr2_1:69765534-69765553 None:intergenic 20.0%
!!! AATTTTCTTTCCTAAAAGAA+GGG - chr2_1:69766646-69766665 Msa0221170:intron 20.0%
!! ATATCATGTTTATTTGTTTG+TGG + chr2_1:69766794-69766813 None:intergenic 20.0%
!! ACTTCATCTTATGATAATAA+GGG - chr2_1:69767088-69767107 Msa0221170:CDS 20.0%
!! TAAAGATTACTTCTTATAGT+GGG + chr2_1:69765375-69765394 None:intergenic 20.0%
!! TTGAATGATGAAAAGAAAAA+GGG + chr2_1:69765533-69765552 None:intergenic 20.0%
!! ATTGAATGATGAAAAGAAAA+AGG + chr2_1:69765534-69765553 None:intergenic 20.0%
!!! AATTTTCTTTCCTAAAAGAA+GGG - chr2_1:69766646-69766665 Msa0221170:intron 20.0%
!! ATATCATGTTTATTTGTTTG+TGG + chr2_1:69766794-69766813 None:intergenic 20.0%
!! ACTTCATCTTATGATAATAA+GGG - chr2_1:69767088-69767107 Msa0221170:CDS 20.0%
! GTAAAGATTACTTCTTATAG+TGG + chr2_1:69765376-69765395 None:intergenic 25.0%
!!! TGTTGTTAATTTGTCATGTT+GGG + chr2_1:69765509-69765528 None:intergenic 25.0%
!!! TTGTTGTTAATTTGTCATGT+TGG + chr2_1:69765510-69765529 None:intergenic 25.0%
! TTATATTTGTGTGTTTGGAA+TGG + chr2_1:69765572-69765591 None:intergenic 25.0%
! TTTGAATTACTAGTTAGTAC+TGG - chr2_1:69766016-69766035 Msa0221170:CDS 25.0%
! TGATTAACTCCAAATATTTG+AGG - chr2_1:69766069-69766088 Msa0221170:intron 25.0%
! GATTAACTCCAAATATTTGA+GGG - chr2_1:69766070-69766089 Msa0221170:intron 25.0%
! ATTAACTCCAAATATTTGAG+GGG - chr2_1:69766071-69766090 Msa0221170:intron 25.0%
! ATTCAAATGACCATAAAAGA+GGG + chr2_1:69766318-69766337 None:intergenic 25.0%
!! CAAAATGTAGTGAGTATTTT+TGG - chr2_1:69766476-69766495 Msa0221170:intron 25.0%
!!! ATGTAGCTTTTAGTTTTCAA+TGG - chr2_1:69766614-69766633 Msa0221170:intron 25.0%
! TTAGGAAAGAAAATTGAACT+TGG + chr2_1:69766641-69766660 None:intergenic 25.0%
!! CAATTTTCTTTCCTAAAAGA+AGG - chr2_1:69766645-69766664 Msa0221170:intron 25.0%
!! TTATTCTATTCCCTTCTTTT+AGG + chr2_1:69766659-69766678 None:intergenic 25.0%
! AAGTATAAGCATAACTAAGT+TGG + chr2_1:69766750-69766769 None:intergenic 25.0%
! AACTTAGTTATGCTTATACT+TGG - chr2_1:69766749-69766768 Msa0221170:intron 25.0%
!!! GTGTACTTTTTTAACTTAGT+TGG - chr2_1:69766845-69766864 Msa0221170:intron 25.0%
! ATGATGCTCTAATTGAATAA+GGG + chr2_1:69767013-69767032 None:intergenic 25.0%
! AATGATGCTCTAATTGAATA+AGG + chr2_1:69767014-69767033 None:intergenic 25.0%
! TCTTATATCAGACAATAAAC+CGG + chr2_1:69767054-69767073 None:intergenic 25.0%
! CACTTCATCTTATGATAATA+AGG - chr2_1:69767087-69767106 Msa0221170:CDS 25.0%
! ATATTCAGTTCTTCATCTTT+AGG - chr2_1:69767302-69767321 Msa0221170:five_prime_UTR 25.0%
! AAAGATTACTTCTTATAGTG+GGG + chr2_1:69765374-69765393 None:intergenic 25.0%
! GTAAAGATTACTTCTTATAG+TGG + chr2_1:69765376-69765395 None:intergenic 25.0%
!!! TGTTGTTAATTTGTCATGTT+GGG + chr2_1:69765509-69765528 None:intergenic 25.0%
!!! TTGTTGTTAATTTGTCATGT+TGG + chr2_1:69765510-69765529 None:intergenic 25.0%
! TTATATTTGTGTGTTTGGAA+TGG + chr2_1:69765572-69765591 None:intergenic 25.0%
! TTTGAATTACTAGTTAGTAC+TGG - chr2_1:69766016-69766035 Msa0221170:CDS 25.0%
! TGATTAACTCCAAATATTTG+AGG - chr2_1:69766069-69766088 Msa0221170:intron 25.0%
! GATTAACTCCAAATATTTGA+GGG - chr2_1:69766070-69766089 Msa0221170:intron 25.0%
! ATTAACTCCAAATATTTGAG+GGG - chr2_1:69766071-69766090 Msa0221170:intron 25.0%
! ATTCAAATGACCATAAAAGA+GGG + chr2_1:69766318-69766337 None:intergenic 25.0%
!! CAAAATGTAGTGAGTATTTT+TGG - chr2_1:69766476-69766495 Msa0221170:intron 25.0%
!!! ATGTAGCTTTTAGTTTTCAA+TGG - chr2_1:69766614-69766633 Msa0221170:intron 25.0%
! TTAGGAAAGAAAATTGAACT+TGG + chr2_1:69766641-69766660 None:intergenic 25.0%
!! CAATTTTCTTTCCTAAAAGA+AGG - chr2_1:69766645-69766664 Msa0221170:intron 25.0%
!! TTATTCTATTCCCTTCTTTT+AGG + chr2_1:69766659-69766678 None:intergenic 25.0%
! AAGTATAAGCATAACTAAGT+TGG + chr2_1:69766750-69766769 None:intergenic 25.0%
! AACTTAGTTATGCTTATACT+TGG - chr2_1:69766749-69766768 Msa0221170:intron 25.0%
!!! GTGTACTTTTTTAACTTAGT+TGG - chr2_1:69766845-69766864 Msa0221170:intron 25.0%
! ATGATGCTCTAATTGAATAA+GGG + chr2_1:69767013-69767032 None:intergenic 25.0%
! AATGATGCTCTAATTGAATA+AGG + chr2_1:69767014-69767033 None:intergenic 25.0%
! TCTTATATCAGACAATAAAC+CGG + chr2_1:69767054-69767073 None:intergenic 25.0%
! CACTTCATCTTATGATAATA+AGG - chr2_1:69767087-69767106 Msa0221170:CDS 25.0%
! ATATTCAGTTCTTCATCTTT+AGG - chr2_1:69767302-69767321 Msa0221170:five_prime_UTR 25.0%
! TATGATGATGATAGTGAGTT+TGG + chr2_1:69765450-69765469 None:intergenic 30.0%
!! GTTGTTAATTTGTCATGTTG+GGG + chr2_1:69765508-69765527 None:intergenic 30.0%
!!! GTGTTGTTTTTCAATGGAAA+TGG - chr2_1:69765683-69765702 Msa0221170:CDS 30.0%
!! ATGAATGATGGTTGTTTTCA+AGG - chr2_1:69765726-69765745 Msa0221170:CDS 30.0%
TTTCAAGGTGTAAATGGAAA+TGG - chr2_1:69765741-69765760 Msa0221170:CDS 30.0%
ATTCTTTCCCCTCAAATATT+TGG + chr2_1:69766081-69766100 None:intergenic 30.0%
!!! GTCTAGTTTTTGTACTTCAT+AGG + chr2_1:69766183-69766202 None:intergenic 30.0%
ACACTTTAACAGGGAATATA+AGG - chr2_1:69766288-69766307 Msa0221170:intron 30.0%
CATTCAAATGACCATAAAAG+AGG + chr2_1:69766319-69766338 None:intergenic 30.0%
! TGCATTTACACTGAAGTTTT+AGG - chr2_1:69766367-69766386 Msa0221170:intron 30.0%
AGTTAAATTGAGACAGTGAA+TGG - chr2_1:69766515-69766534 Msa0221170:intron 30.0%
! ACAGTGAATGGCAAAATTTT+GGG - chr2_1:69766527-69766546 Msa0221170:intron 30.0%
! AATGGCTTTACTCACATATA+TGG + chr2_1:69766555-69766574 None:intergenic 30.0%
GACTTCAAAAGTAAGCAAAA+TGG + chr2_1:69766573-69766592 None:intergenic 30.0%
TGTAATAACTTCAGCTAGTA+AGG + chr2_1:69766880-69766899 None:intergenic 30.0%
ATAAGAGTTGAATCACAAGA+AGG - chr2_1:69766972-69766991 Msa0221170:CDS 30.0%
TTATGTTGTGAATACAAACC+CGG - chr2_1:69767032-69767051 Msa0221170:CDS 30.0%
CTTATATCAGACAATAAACC+GGG + chr2_1:69767053-69767072 None:intergenic 30.0%
TCTTAGAAACTAGAAAGAGA+AGG - chr2_1:69767270-69767289 Msa0221170:five_prime_UTR 30.0%
TTCACATGTAATGTGAATCT+TGG - chr2_1:69767357-69767376 Msa0221170:five_prime_UTR 30.0%
TCACATGTAATGTGAATCTT+GGG - chr2_1:69767358-69767377 Msa0221170:five_prime_UTR 30.0%
ACAATGAATGAACTCATTCT+AGG - chr2_1:69767461-69767480 Msa0221170:five_prime_UTR 30.0%
AAGATTACTTCTTATAGTGG+GGG + chr2_1:69765373-69765392 None:intergenic 30.0%
! TATGATGATGATAGTGAGTT+TGG + chr2_1:69765450-69765469 None:intergenic 30.0%
!! GTTGTTAATTTGTCATGTTG+GGG + chr2_1:69765508-69765527 None:intergenic 30.0%
!!! GTGTTGTTTTTCAATGGAAA+TGG - chr2_1:69765683-69765702 Msa0221170:CDS 30.0%
!! ATGAATGATGGTTGTTTTCA+AGG - chr2_1:69765726-69765745 Msa0221170:CDS 30.0%
TTTCAAGGTGTAAATGGAAA+TGG - chr2_1:69765741-69765760 Msa0221170:CDS 30.0%
ATTCTTTCCCCTCAAATATT+TGG + chr2_1:69766081-69766100 None:intergenic 30.0%
!!! GTCTAGTTTTTGTACTTCAT+AGG + chr2_1:69766183-69766202 None:intergenic 30.0%
ACACTTTAACAGGGAATATA+AGG - chr2_1:69766288-69766307 Msa0221170:intron 30.0%
CATTCAAATGACCATAAAAG+AGG + chr2_1:69766319-69766338 None:intergenic 30.0%
! TGCATTTACACTGAAGTTTT+AGG - chr2_1:69766367-69766386 Msa0221170:intron 30.0%
AGTTAAATTGAGACAGTGAA+TGG - chr2_1:69766515-69766534 Msa0221170:intron 30.0%
! ACAGTGAATGGCAAAATTTT+GGG - chr2_1:69766527-69766546 Msa0221170:intron 30.0%
! AATGGCTTTACTCACATATA+TGG + chr2_1:69766555-69766574 None:intergenic 30.0%
GACTTCAAAAGTAAGCAAAA+TGG + chr2_1:69766573-69766592 None:intergenic 30.0%
TGTAATAACTTCAGCTAGTA+AGG + chr2_1:69766880-69766899 None:intergenic 30.0%
ATAAGAGTTGAATCACAAGA+AGG - chr2_1:69766972-69766991 Msa0221170:CDS 30.0%
TTATGTTGTGAATACAAACC+CGG - chr2_1:69767032-69767051 Msa0221170:CDS 30.0%
CTTATATCAGACAATAAACC+GGG + chr2_1:69767053-69767072 None:intergenic 30.0%
TCTTAGAAACTAGAAAGAGA+AGG - chr2_1:69767270-69767289 Msa0221170:five_prime_UTR 30.0%
TTCACATGTAATGTGAATCT+TGG - chr2_1:69767357-69767376 Msa0221170:five_prime_UTR 30.0%
TCACATGTAATGTGAATCTT+GGG - chr2_1:69767358-69767377 Msa0221170:five_prime_UTR 30.0%
ACAATGAATGAACTCATTCT+AGG - chr2_1:69767461-69767480 Msa0221170:five_prime_UTR 30.0%
!! TTAATTTGTCATGTTGGGGT+TGG + chr2_1:69765504-69765523 None:intergenic 35.0%
GGCTGTTATATTTGTGTGTT+TGG + chr2_1:69765577-69765596 None:intergenic 35.0%
!!! GGAACTGTGTTGTTTTTCAA+TGG - chr2_1:69765677-69765696 Msa0221170:CDS 35.0%
GCTTCAAGTAGAATGAATGA+TGG - chr2_1:69765714-69765733 Msa0221170:CDS 35.0%
!! GGTTGTTTTCAAGGTGTAAA+TGG - chr2_1:69765735-69765754 Msa0221170:CDS 35.0%
GGTGTAAATGGAAATGGATA+TGG - chr2_1:69765747-69765766 Msa0221170:CDS 35.0%
ATATGGTTCATCTTCTTCAC+TGG - chr2_1:69765764-69765783 Msa0221170:CDS 35.0%
!!! TTCATCTTCTTCACTGGTTT+TGG - chr2_1:69765770-69765789 Msa0221170:CDS 35.0%
!!! CTGGTTTTGGATAGAGAAAA+AGG - chr2_1:69765783-69765802 Msa0221170:CDS 35.0%
AAAGCTTCAATACTTCTCTC+AGG + chr2_1:69765855-69765874 None:intergenic 35.0%
AACTAACCTTATTAGCACCT+GGG + chr2_1:69765953-69765972 None:intergenic 35.0%
AAGACTCACACACTTTAACA+GGG - chr2_1:69766279-69766298 Msa0221170:intron 35.0%
! GACAGTGAATGGCAAAATTT+TGG - chr2_1:69766526-69766545 Msa0221170:intron 35.0%
! CTTATCTCATCACTGTCTTT+TGG + chr2_1:69766957-69766976 None:intergenic 35.0%
!! TCACAAGAAGGTGGATTAAA+TGG - chr2_1:69766984-69767003 Msa0221170:CDS 35.0%
TAGAAACTAGAAAGAGAAGG+AGG - chr2_1:69767273-69767292 Msa0221170:five_prime_UTR 35.0%
! ATCTTGGGTTGTACATTTGT+TGG - chr2_1:69767373-69767392 Msa0221170:five_prime_UTR 35.0%
! TCTTGGGTTGTACATTTGTT+GGG - chr2_1:69767374-69767393 Msa0221170:five_prime_UTR 35.0%
! GGGAACTTTTGTAGATAGTT+AGG - chr2_1:69767394-69767413 Msa0221170:five_prime_UTR 35.0%
! AGATTACTTCTTATAGTGGG+GGG + chr2_1:69765372-69765391 None:intergenic 35.0%
!! TTAATTTGTCATGTTGGGGT+TGG + chr2_1:69765504-69765523 None:intergenic 35.0%
GGCTGTTATATTTGTGTGTT+TGG + chr2_1:69765577-69765596 None:intergenic 35.0%
!!! GGAACTGTGTTGTTTTTCAA+TGG - chr2_1:69765677-69765696 Msa0221170:CDS 35.0%
GCTTCAAGTAGAATGAATGA+TGG - chr2_1:69765714-69765733 Msa0221170:CDS 35.0%
!! GGTTGTTTTCAAGGTGTAAA+TGG - chr2_1:69765735-69765754 Msa0221170:CDS 35.0%
GGTGTAAATGGAAATGGATA+TGG - chr2_1:69765747-69765766 Msa0221170:CDS 35.0%
ATATGGTTCATCTTCTTCAC+TGG - chr2_1:69765764-69765783 Msa0221170:CDS 35.0%
!!! TTCATCTTCTTCACTGGTTT+TGG - chr2_1:69765770-69765789 Msa0221170:CDS 35.0%
!!! CTGGTTTTGGATAGAGAAAA+AGG - chr2_1:69765783-69765802 Msa0221170:CDS 35.0%
AAAGCTTCAATACTTCTCTC+AGG + chr2_1:69765855-69765874 None:intergenic 35.0%
AACTAACCTTATTAGCACCT+GGG + chr2_1:69765953-69765972 None:intergenic 35.0%
AAGACTCACACACTTTAACA+GGG - chr2_1:69766279-69766298 Msa0221170:intron 35.0%
! GACAGTGAATGGCAAAATTT+TGG - chr2_1:69766526-69766545 Msa0221170:intron 35.0%
! CTTATCTCATCACTGTCTTT+TGG + chr2_1:69766957-69766976 None:intergenic 35.0%
!! TCACAAGAAGGTGGATTAAA+TGG - chr2_1:69766984-69767003 Msa0221170:CDS 35.0%
TAGAAACTAGAAAGAGAAGG+AGG - chr2_1:69767273-69767292 Msa0221170:five_prime_UTR 35.0%
! ATCTTGGGTTGTACATTTGT+TGG - chr2_1:69767373-69767392 Msa0221170:five_prime_UTR 35.0%
! TCTTGGGTTGTACATTTGTT+GGG - chr2_1:69767374-69767393 Msa0221170:five_prime_UTR 35.0%
! GGGAACTTTTGTAGATAGTT+AGG - chr2_1:69767394-69767413 Msa0221170:five_prime_UTR 35.0%
!! TTTGCTGTTGATGCTTCTTC+TGG + chr2_1:69765398-69765417 None:intergenic 40.0%
AAGACAACGTTGGAAGAAGA+AGG + chr2_1:69765613-69765632 None:intergenic 40.0%
GACACAAAGAAAGACAACGT+TGG + chr2_1:69765623-69765642 None:intergenic 40.0%
CATATTCTGAACACCCTCTT+GGG - chr2_1:69765656-69765675 Msa0221170:CDS 40.0%
AAACAACACAGTTCCCAAGA+GGG + chr2_1:69765672-69765691 None:intergenic 40.0%
AAAACAACACAGTTCCCAAG+AGG + chr2_1:69765673-69765692 None:intergenic 40.0%
TAGAGAAAAAGGAGAGCTTG+TGG - chr2_1:69765794-69765813 Msa0221170:CDS 40.0%
AGAATCATAGTGAAGCAGAG+AGG - chr2_1:69765877-69765896 Msa0221170:CDS 40.0%
TGTTATCCCAGGTGCTAATA+AGG - chr2_1:69765944-69765963 Msa0221170:CDS 40.0%
GAACTAACCTTATTAGCACC+TGG + chr2_1:69765954-69765973 None:intergenic 40.0%
TAAAGTGTGTGAGTCTTCAC+AGG + chr2_1:69766276-69766295 None:intergenic 40.0%
GAAGACTCACACACTTTAAC+AGG - chr2_1:69766278-69766297 Msa0221170:intron 40.0%
! ATAAGGACGACCCTCTTTTA+TGG - chr2_1:69766305-69766324 Msa0221170:intron 40.0%
CAAGAGCTCAATGAGTTTGA+TGG - chr2_1:69766695-69766714 Msa0221170:intron 40.0%
AGAGTTGAATCACAAGAAGG+TGG - chr2_1:69766975-69766994 Msa0221170:CDS 40.0%
AGCAAGAAACTGTCGATACT+CGG + chr2_1:69767192-69767211 None:intergenic 40.0%
GGAATAGAAGTTATGTCAGC+AGG + chr2_1:69767514-69767533 None:intergenic 40.0%
!! TTTGCTGTTGATGCTTCTTC+TGG + chr2_1:69765398-69765417 None:intergenic 40.0%
AAGACAACGTTGGAAGAAGA+AGG + chr2_1:69765613-69765632 None:intergenic 40.0%
GACACAAAGAAAGACAACGT+TGG + chr2_1:69765623-69765642 None:intergenic 40.0%
CATATTCTGAACACCCTCTT+GGG - chr2_1:69765656-69765675 Msa0221170:CDS 40.0%
AAACAACACAGTTCCCAAGA+GGG + chr2_1:69765672-69765691 None:intergenic 40.0%
AAAACAACACAGTTCCCAAG+AGG + chr2_1:69765673-69765692 None:intergenic 40.0%
TAGAGAAAAAGGAGAGCTTG+TGG - chr2_1:69765794-69765813 Msa0221170:CDS 40.0%
AGAATCATAGTGAAGCAGAG+AGG - chr2_1:69765877-69765896 Msa0221170:CDS 40.0%
TGTTATCCCAGGTGCTAATA+AGG - chr2_1:69765944-69765963 Msa0221170:CDS 40.0%
GAACTAACCTTATTAGCACC+TGG + chr2_1:69765954-69765973 None:intergenic 40.0%
TAAAGTGTGTGAGTCTTCAC+AGG + chr2_1:69766276-69766295 None:intergenic 40.0%
GAAGACTCACACACTTTAAC+AGG - chr2_1:69766278-69766297 Msa0221170:intron 40.0%
! ATAAGGACGACCCTCTTTTA+TGG - chr2_1:69766305-69766324 Msa0221170:intron 40.0%
CAAGAGCTCAATGAGTTTGA+TGG - chr2_1:69766695-69766714 Msa0221170:intron 40.0%
AGAGTTGAATCACAAGAAGG+TGG - chr2_1:69766975-69766994 Msa0221170:CDS 40.0%
AGCAAGAAACTGTCGATACT+CGG + chr2_1:69767192-69767211 None:intergenic 40.0%
GGAATAGAAGTTATGTCAGC+AGG + chr2_1:69767514-69767533 None:intergenic 40.0%
TGTTGGGGTTGGAGTGTATT+TGG + chr2_1:69765493-69765512 None:intergenic 45.0%
GAAGAAGGATGAGGAGACAA+AGG + chr2_1:69765598-69765617 None:intergenic 45.0%
ACGTTGGAAGAAGAAGGATG+AGG + chr2_1:69765607-69765626 None:intergenic 45.0%
!! CCAAGAGGGTGTTCAGAATA+TGG + chr2_1:69765658-69765677 None:intergenic 45.0%
CCATATTCTGAACACCCTCT+TGG - chr2_1:69765655-69765674 Msa0221170:CDS 45.0%
AGAAAAAGGAGAGCTTGTGG+AGG - chr2_1:69765797-69765816 Msa0221170:CDS 45.0%
GCTGTGAAATTGGAACGCAA+AGG - chr2_1:69765822-69765841 Msa0221170:CDS 45.0%
ATCATAGTGAAGCAGAGAGG+AGG - chr2_1:69765880-69765899 Msa0221170:CDS 45.0%
ACACTTCGTAGTGTTATCCC+AGG - chr2_1:69765933-69765952 Msa0221170:CDS 45.0%
AATGCAGCACAAGCAAGTGA+AGG - chr2_1:69766921-69766940 Msa0221170:intron 45.0%
ATGCAGCACAAGCAAGTGAA+GGG - chr2_1:69766922-69766941 Msa0221170:intron 45.0%
AAGGGCAGAGATTGCAACTT+TGG - chr2_1:69767106-69767125 Msa0221170:CDS 45.0%
AGGGCAGAGATTGCAACTTT+GGG - chr2_1:69767107-69767126 Msa0221170:CDS 45.0%
!! GAGTATCGACAGTTTCTTGC+TGG - chr2_1:69767191-69767210 Msa0221170:CDS 45.0%
TCGACAGTTTCTTGCTGGAT+CGG - chr2_1:69767196-69767215 Msa0221170:exon 45.0%
TGTTGGGGTTGGAGTGTATT+TGG + chr2_1:69765493-69765512 None:intergenic 45.0%
GAAGAAGGATGAGGAGACAA+AGG + chr2_1:69765598-69765617 None:intergenic 45.0%
ACGTTGGAAGAAGAAGGATG+AGG + chr2_1:69765607-69765626 None:intergenic 45.0%
!! CCAAGAGGGTGTTCAGAATA+TGG + chr2_1:69765658-69765677 None:intergenic 45.0%
CCATATTCTGAACACCCTCT+TGG - chr2_1:69765655-69765674 Msa0221170:CDS 45.0%
AGAAAAAGGAGAGCTTGTGG+AGG - chr2_1:69765797-69765816 Msa0221170:CDS 45.0%
GCTGTGAAATTGGAACGCAA+AGG - chr2_1:69765822-69765841 Msa0221170:CDS 45.0%
ATCATAGTGAAGCAGAGAGG+AGG - chr2_1:69765880-69765899 Msa0221170:CDS 45.0%
ACACTTCGTAGTGTTATCCC+AGG - chr2_1:69765933-69765952 Msa0221170:CDS 45.0%
AATGCAGCACAAGCAAGTGA+AGG - chr2_1:69766921-69766940 Msa0221170:intron 45.0%
ATGCAGCACAAGCAAGTGAA+GGG - chr2_1:69766922-69766941 Msa0221170:intron 45.0%
AAGGGCAGAGATTGCAACTT+TGG - chr2_1:69767106-69767125 Msa0221170:CDS 45.0%
AGGGCAGAGATTGCAACTTT+GGG - chr2_1:69767107-69767126 Msa0221170:CDS 45.0%
!! GAGTATCGACAGTTTCTTGC+TGG - chr2_1:69767191-69767210 Msa0221170:CDS 45.0%
TCGACAGTTTCTTGCTGGAT+CGG - chr2_1:69767196-69767215 Msa0221170:exon 45.0%
! GTGAGTTTGGTGAGATCCAG+AGG + chr2_1:69765437-69765456 None:intergenic 50.0%
TGTGGAGGCAGCTGTGAAAT+TGG - chr2_1:69765812-69765831 Msa0221170:CDS 50.0%
GCAGAGATTGCAACTTTGGG+AGG - chr2_1:69767110-69767129 Msa0221170:CDS 50.0%
! GTGAGTTTGGTGAGATCCAG+AGG + chr2_1:69765437-69765456 None:intergenic 50.0%
TGTGGAGGCAGCTGTGAAAT+TGG - chr2_1:69765812-69765831 Msa0221170:CDS 50.0%
GCAGAGATTGCAACTTTGGG+AGG - chr2_1:69767110-69767129 Msa0221170:CDS 50.0%
AGCACTACTCTCTCTGCCTC+TGG - chr2_1:69765418-69765437 Msa0221170:three_prime_UTR 55.0%
AGCACTACTCTCTCTGCCTC+TGG - chr2_1:69765418-69765437 Msa0221170:three_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 69765372 69767534 69765372 ID=Msa0221170;Name=Msa0221170
chr2_1 mRNA 69765372 69767534 69765372 ID=Msa0221170-mRNA-1;Parent=Msa0221170;Name=Msa0221170-mRNA-1;_AED=0.00;_eAED=0.00;_QI=324|1|1|1|1|1|2|197|247
chr2_1 exon 69765372 69766042 69765372 ID=Msa0221170-mRNA-1:exon:19772;Parent=Msa0221170-mRNA-1
chr2_1 exon 69766941 69767534 69766941 ID=Msa0221170-mRNA-1:exon:19771;Parent=Msa0221170-mRNA-1
chr2_1 five_prime_UTR 69767211 69767534 69767211 ID=Msa0221170-mRNA-1:five_prime_utr;Parent=Msa0221170-mRNA-1
chr2_1 CDS 69766941 69767210 69766941 ID=Msa0221170-mRNA-1:cds;Parent=Msa0221170-mRNA-1
chr2_1 CDS 69765569 69766042 69765569 ID=Msa0221170-mRNA-1:cds;Parent=Msa0221170-mRNA-1
chr2_1 three_prime_UTR 69765372 69765568 69765372 ID=Msa0221170-mRNA-1:three_prime_utr;Parent=Msa0221170-mRNA-1
Gene Sequence

>Msa0221170

ATGGAAATGGATGATTTTGCTTCAAGTAGAATGAATGATGGTTGTTTTCAAGGTGTAAATGGAAATGGATATGGTTCATCTTCTTCACTGGTTTTGGATAGAGAAAAAGGAGAGCTTGTGGAGGCAGCTGTGAAATTGGAACGCAAAGGTGTGTCACCTGAGAGAAGTATTGAAGCTTTGAAGAATCATAGTGAAGCAGAGAGGAGGAGAAGAGCAAGAATTAATTCACATCTTGATACACTTCGTAGTGTTATCCCAGGTGCTAATAAGTTGGACAAAGCATCCTTACTAGCTGAAGTTATTACACATTTGAAAGAGCTGAAAACAAATGCAGCACAAGCAAGTGAAGGGTTCATTATTCCAAAAGACAGTGATGAGATAAGAGTTGAATCACAAGAAGGTGGATTAAATGGTTACCCTTATTCAATTAGAGCATCATTATGTTGTGAATACAAACCCGGTTTATTGTCTGATATAAGAAAAGCACTTGATGCACTTCATCTTATGATAATAAGGGCAGAGATTGCAACTTTGGGAGGTAGAATGAAGAATGTCTTTGTCATAATTAGCTGCAAAGAACAGAATTTTGAAGATGCCGAGTATCGACAGTTTCTTGCTGGATCGGTTCATCAAGCTCTTAGATCTGTACTTGATAGATTTTCTGTTTCACAAGATATCTTAGAAACTAGAAAGAGAAGGAGGATTTCTATATTCAGTTCTTCATCTTTAGGTGATTTCTTATAA
Protein sequence

>Msa0221170

MEMDDFASSRMNDGCFQGVNGNGYGSSSSLVLDREKGELVEAAVKLERKGVSPERSIEALKNHSEAERRRRARINSHLDTLRSVIPGANKLDKASLLAEVITHLKELKTNAAQASEGFIIPKDSDEIRVESQEGGLNGYPYSIRASLCCEYKPGLLSDIRKALDALHLMIIRAEIATLGGRMKNVFVIISCKEQNFEDAEYRQFLAGSVHQALRSVLDRFSVSQDILETRKRRRISIFSSSSLGDFL*