AlfalfaGEDB | Msa0224090

M. sativa cultivar zhongmu-4 / Msa0224090

KEGG: K09422
GO: GO:0000228 GO:0000723 GO:0000781 GO:0000784 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003691 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006355 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0006996 GO:0008150 GO:0008152 GO:0009628 GO:0009651 GO:0009719 GO:0009723 GO:0009725 GO:0009733 GO:0009751 GO:0009753 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010468 GO:0010556 GO:0014070 GO:0016043 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0031627 GO:0031974 GO:0031981 GO:0032200 GO:0034641 GO:0042162 GO:0042221 GO:0042493 GO:0042592 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0046677 GO:0046686 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051276 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0098687 GO:0140110 GO:1901360 GO:1901363 GO:1901700 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00249.34

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0224090	AES67811.1	57.609	92	27	2	1	92	1	80	2.22e-24	98.2

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0224090	sp\|F4I7L1\|TRB4_ARATH	59.722	72	20	2	1	63	1	72	1.18e-19	83.6

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0224090	G7IS31	57.609	92	27	2	1	92	1	80	1.06e-24	98.2

TFs/TRs:

Gene ID	Type	Classification
Msa0224090	TF	MYB-related

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0224090	MtrunA17_Chr2g0329921	51.648	91	40	2	1	87	1	91	9.81e-22	86.3
Msa0224090	MtrunA17_Chr8g0383821	62.963	54	20	0	4	57	6	59	7.67e-19	75.1
Msa0224090	MtrunA17_Chr8g0384671	50.000	76	38	0	4	79	6	81	3.14e-18	76.6
Msa0224090	MtrunA17_Chr1g0166681	67.308	52	17	0	4	55	6	57	4.04e-18	74.7
Msa0224090	MtrunA17_Chr8g0342871	55.385	65	27	1	1	63	1	65	1.42e-17	74.7
Msa0224090	MtrunA17_Chr2g0293381	63.462	52	19	0	4	55	6	57	3.55e-17	73.9
Msa0224090	MtrunA17_Chr4g0054131	62.745	51	19	0	4	54	6	56	1.53e-16	72.4
Msa0224090	MtrunA17_Chr5g0401061	62.745	51	19	0	4	54	6	56	2.00e-16	72.0
Msa0224090	MtrunA17_Chr1g0195261	53.333	45	21	0	4	48	6	50	2.96e-11	53.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0224090	AT1G17520.1	59.722	72	20	2	1	63	1	72	1.20e-20	83.6
Msa0224090	AT1G17520.4	59.722	72	20	2	1	63	1	72	1.27e-20	83.6
Msa0224090	AT1G17520.2	59.722	72	20	2	1	63	1	72	1.28e-20	83.6
Msa0224090	AT1G17520.3	59.722	72	20	2	1	63	1	72	1.29e-20	83.6
Msa0224090	AT1G72740.1	61.404	57	20	1	1	55	1	57	3.21e-18	77.0
Msa0224090	AT1G72740.2	61.404	57	20	1	1	55	1	57	3.47e-18	76.6
Msa0224090	AT1G49950.3	61.538	52	20	0	4	55	6	57	4.31e-16	71.2
Msa0224090	AT1G49950.1	61.538	52	20	0	4	55	6	57	4.31e-16	71.2
Msa0224090	AT1G49950.2	61.538	52	20	0	4	55	6	57	4.31e-16	71.2
Msa0224090	AT5G67580.2	61.538	52	20	0	4	55	6	57	3.52e-15	68.9
Msa0224090	AT5G67580.1	61.538	52	20	0	4	55	6	57	3.52e-15	68.9
Msa0224090	AT3G49850.1	60.784	51	20	0	5	55	7	57	1.43e-14	67.0
Msa0224090	AT3G49850.2	60.784	51	20	0	5	55	7	57	1.43e-14	67.0

Find 17 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGGCAGAATTTAACTTCTA+AGG	0.272609	2_1:+73831487	Msa0224090:CDS
TGATTGTTTACCAGGATAAA+TGG	0.284224	2_1:+73831468	Msa0224090:intron
ACAGAAGAAGCGTTAGAAAA+AGG	0.338386	2_1:+73831247	Msa0224090:CDS
TACAAGGTGTTACACGATAA+TGG	0.364573	2_1:+73831600	Msa0224090:CDS
TCCTTATCATGCTGCTTCTT+CGG	0.379902	2_1:+73831523	Msa0224090:CDS
AGAAATACGGCGTCGGAAAG+TGG	0.422049	2_1:+73831275	Msa0224090:CDS
GCGAGTATTTGACCGAGCTC+AGG	0.447776	2_1:-73831316	None:intergenic
ACCGAAGAAGCAGCATGATA+AGG	0.505859	2_1:-73831524	None:intergenic
CTAGCCAATGCTACTCTACA+AGG	0.513732	2_1:+73831584	Msa0224090:CDS
AACAACCTTGAGATCAGTGT+TGG	0.530301	2_1:-73831347	None:intergenic
GAAATACGGCGTCGGAAAGT+GGG	0.534166	2_1:+73831276	Msa0224090:CDS
AACACCTTGTAGAGTAGCAT+TGG	0.543073	2_1:-73831588	None:intergenic
AAGAGATGGGAAATCAAAAG+TGG	0.558180	2_1:+73831215	None:intergenic
CGAGTATTTGACCGAGCTCA+GGG	0.577095	2_1:-73831315	None:intergenic
TCGTTCCAACACTGATCTCA+AGG	0.583235	2_1:+73831342	Msa0224090:CDS
GCATTGGCTAGAGCTAGAGC+AGG	0.593031	2_1:-73831572	None:intergenic
GGTGTCAAGAAATACGGCGT+CGG	0.659365	2_1:+73831268	Msa0224090:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TACTTCTCTAATTAATATTA+GGG	+	chr2_1:73831377-73831396	Msa0224090:intron	15.0%
!!	CTACTTCTCTAATTAATATT+AGG	+	chr2_1:73831376-73831395	Msa0224090:intron	20.0%
!!	TCTAATTAATATTAGGGTTA+GGG	+	chr2_1:73831383-73831402	Msa0224090:intron	20.0%
!	CTCTAATTAATATTAGGGTT+AGG	+	chr2_1:73831382-73831401	Msa0224090:intron	25.0%
!	TCAATTGTGTCTATCATTAT+TGG	+	chr2_1:73831438-73831457	Msa0224090:intron	25.0%
!	GTTATAATTGATTGTTTACC+AGG	+	chr2_1:73831460-73831479	Msa0224090:intron	25.0%
!!	GAAAAAGGTGTCAAGAAATA+CGG	+	chr2_1:73831262-73831281	Msa0224090:CDS	30.0%
	TGATTGTTTACCAGGATAAA+TGG	+	chr2_1:73831468-73831487	Msa0224090:intron	30.0%
!	TTAAATTCTGCCATTTATCC+TGG	-	chr2_1:73831481-73831500	None:intergenic	30.0%
!	ATGGCAGAATTTAACTTCTA+AGG	+	chr2_1:73831487-73831506	Msa0224090:CDS	30.0%
!!	ACAGAAGAAGCGTTAGAAAA+AGG	+	chr2_1:73831247-73831266	Msa0224090:CDS	35.0%
!!	ATTTTGAAAGACCCTGAGCT+CGG	+	chr2_1:73831304-73831323	Msa0224090:CDS	40.0%
	AACAACCTTGAGATCAGTGT+TGG	-	chr2_1:73831350-73831369	None:intergenic	40.0%
!	TCCTTATCATGCTGCTTCTT+CGG	+	chr2_1:73831523-73831542	Msa0224090:CDS	40.0%
	AACACCTTGTAGAGTAGCAT+TGG	-	chr2_1:73831591-73831610	None:intergenic	40.0%
	TCGTTCCAACACTGATCTCA+AGG	+	chr2_1:73831342-73831361	Msa0224090:CDS	45.0%
	ACCGAAGAAGCAGCATGATA+AGG	-	chr2_1:73831527-73831546	None:intergenic	45.0%
	CTAGCCAATGCTACTCTACA+AGG	+	chr2_1:73831584-73831603	Msa0224090:CDS	45.0%
	GGTGTCAAGAAATACGGCGT+CGG	+	chr2_1:73831268-73831287	Msa0224090:CDS	50.0%
!	AGAAATACGGCGTCGGAAAG+TGG	+	chr2_1:73831275-73831294	Msa0224090:CDS	50.0%
!	GAAATACGGCGTCGGAAAGT+GGG	+	chr2_1:73831276-73831295	Msa0224090:CDS	50.0%
	CGAGTATTTGACCGAGCTCA+GGG	-	chr2_1:73831318-73831337	None:intergenic	50.0%
	GCGAGTATTTGACCGAGCTC+AGG	-	chr2_1:73831319-73831338	None:intergenic	55.0%
!!	GCATTGGCTAGAGCTAGAGC+AGG	-	chr2_1:73831575-73831594	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	73831220	73831619	73831220	ID=Msa0224090;Name=Msa0224090
chr2_1	mRNA	73831220	73831619	73831220	ID=Msa0224090-mRNA-1;Parent=Msa0224090;Name=Msa0224090-mRNA-1;_AED=0.56;_eAED=0.71;_QI=0\|0\|0\|1\|1\|1\|2\|0\|93
chr2_1	exon	73831220	73831363	73831220	ID=Msa0224090-mRNA-1:exon:21747;Parent=Msa0224090-mRNA-1
chr2_1	exon	73831482	73831619	73831482	ID=Msa0224090-mRNA-1:exon:21748;Parent=Msa0224090-mRNA-1
chr2_1	CDS	73831220	73831363	73831220	ID=Msa0224090-mRNA-1:cds;Parent=Msa0224090-mRNA-1
chr2_1	CDS	73831482	73831619	73831482	ID=Msa0224090-mRNA-1:cds;Parent=Msa0224090-mRNA-1

Gene Sequence	>Msa0224090 ATGGGAAATCAAAAGTGGACATCTGAAACAGAAGAAGCGTTAGAAAAAGGTGTCAAGAAATACGGCGTCGGAAAGTGGGCAGATATTTTGAAAGACCCTGAGCTCGGTCAAATACTCGCTAGTCGTTCCAACACTGATCTCAAGGATAAATGGCAGAATTTAACTTCTAAGGACAAATCATTTGTTCCTTATCATGCTGCTTCTTCGGTCAATGTTGCTTTGTCTCATGTTGTTCCTGCTCTAGCTCTAGCCAATGCTACTCTACAAGGTGTTACACGATAA
Protein sequence	>Msa0224090 MGNQKWTSETEEALEKGVKKYGVGKWADILKDPELGQILASRSNTDLKDKWQNLTSKDKSFVPYHAASSVNVALSHVVPALALANATLQGVTR*