AlfalfaGEDB | Msa0227150

M. sativa cultivar zhongmu-4 / Msa0227150

KEGG: -
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00010.29

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0227150	XP_039685450.1	80.556	180	20	1	1	165	14	193	1.61e-93	284

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0227150	sp\|Q9T072\|BH025_ARATH	37.391	115	68	2	55	165	105	219	5.57e-16	77.0

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0227150	A0A072TRP6	80.447	179	20	1	2	165	1	179	1.48e-93	281

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TFs/TRs:

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Gene ID	Type	Classification
Msa0227150	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0227150	MtrunA17_Chr2g0333541	90.244	164	13	1	2	165	1	161	3.69e-94	274
Msa0227150	MtrunA17_Chr8g0368371	78.723	141	15	1	2	127	1	141	1.20e-73	217
Msa0227150	MtrunA17_Chr2g0333641	41.279	172	74	6	5	152	23	191	1.61e-25	99.4
Msa0227150	MtrunA17_Chr2g0333621	39.153	189	81	6	2	162	20	202	5.37e-25	98.2
Msa0227150	MtrunA17_Chr8g0336681	59.155	71	29	0	87	157	145	215	3.21e-21	88.6
Msa0227150	MtrunA17_Chr2g0333611	47.115	104	52	3	65	167	13	114	6.79e-21	85.5
Msa0227150	MtrunA17_Chr4g0002801	47.959	98	46	1	60	152	109	206	6.28e-20	84.7
Msa0227150	MtrunA17_Chr7g0249811	59.091	66	26	1	87	151	153	218	2.49e-19	83.2
Msa0227150	MtrunA17_Chr4g0002781	54.795	73	33	0	80	152	156	228	1.05e-18	81.6
Msa0227150	MtrunA17_Chr5g0400621	47.619	84	42	1	83	166	144	225	7.55e-17	76.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0227150	AT4G37850.2	37.391	115	68	2	55	165	105	219	5.66e-17	77.0
Msa0227150	AT4G37850.1	37.391	115	68	2	55	165	105	219	5.66e-17	77.0
Msa0227150	AT2G22770.1	51.786	56	27	0	98	153	132	187	3.81e-13	66.2
Msa0227150	AT2G22750.1	52.727	55	26	0	91	145	119	173	6.12e-13	65.5
Msa0227150	AT2G22750.2	52.727	55	26	0	91	145	119	173	6.47e-13	65.5
Msa0227150	AT2G22750.4	52.727	55	26	0	91	145	147	201	7.41e-13	65.5
Msa0227150	AT2G22750.5	52.727	55	26	0	91	145	143	197	7.53e-13	65.5
Msa0227150	AT2G22750.3	52.727	55	26	0	91	145	147	201	7.66e-13	65.5
Msa0227150	AT2G22760.2	46.032	63	34	0	91	153	112	174	8.73e-12	62.0
Msa0227150	AT2G22760.1	46.032	63	34	0	91	153	112	174	9.32e-12	62.0

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Find 32 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGTAAGATCATAGAACTTT+CGG	0.375512	2_1:+77837385	Msa0227150:CDS
AATGGCTGTGTAGATTGATA+AGG	0.480377	2_1:+77837528	Msa0227150:intron
AATGTCTCAGAAGCGCTTCG+AGG	0.506140	2_1:-77837320	None:intergenic
ACATAATGTCGGAGAGGAAT+AGG	0.390434	2_1:+77837348	Msa0227150:CDS
ACTAGGCCAGTTGTGGCAAT+AGG	0.327076	2_1:-77837149	None:intergenic
AGTGTCCCTATTGCCACAAC+TGG	0.491296	2_1:+77837143	Msa0227150:CDS
AGTTGAATGTGACACATTGA+TGG	0.456224	2_1:-77837183	None:intergenic
ATACTAACCTTCTTCAATCC+AGG	0.486683	2_1:-77837416	None:intergenic
ATGACAAAGGCAAAGAATCA+TGG	0.529186	2_1:-77837262	None:intergenic
CATCATCAACGGATAACCAA+GGG	0.664890	2_1:+77837282	Msa0227150:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GACATTAGATCACATAATGT+CGG	+	chr2_1:77837337-77837356	Msa0227150:CDS	30.0%
AGTTGAATGTGACACATTGA+TGG	-	chr2_1:77837186-77837205	None:intergenic	35.0%
ATGACAAAGGCAAAGAATCA+TGG	-	chr2_1:77837265-77837284	None:intergenic	35.0%
TTTGCCTTTGTCATCATCAA+CGG	+	chr2_1:77837271-77837290	Msa0227150:CDS	35.0%
CTTCTTCAATCCAGGTATAA+AGG	-	chr2_1:77837411-77837430	None:intergenic	35.0%
CTTTATACCTGGATTGAAGA+AGG	+	chr2_1:77837409-77837428	Msa0227150:CDS	35.0%
ATACTAACCTTCTTCAATCC+AGG	-	chr2_1:77837419-77837438	None:intergenic	35.0%
AATGGCTGTGTAGATTGATA+AGG	+	chr2_1:77837528-77837547	Msa0227150:intron	35.0%
GTAGATTGATAAGGTTCATG+TGG	+	chr2_1:77837537-77837556	Msa0227150:intron	35.0%
TGATAAGGTTCATGTGGTAA+CGG	+	chr2_1:77837543-77837562	Msa0227150:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	77837053	77837672	77837053	ID=Msa0227150;Name=Msa0227150
chr2_1	mRNA	77837053	77837672	77837053	ID=Msa0227150-mRNA-1;Parent=Msa0227150;Name=Msa0227150-mRNA-1;_AED=0.21;_eAED=0.23;_QI=0\|0\|0\|1\|1\|1\|2\|0\|169
chr2_1	exon	77837053	77837430	77837053	ID=Msa0227150-mRNA-1:exon:23707;Parent=Msa0227150-mRNA-1
chr2_1	exon	77837541	77837672	77837541	ID=Msa0227150-mRNA-1:exon:23708;Parent=Msa0227150-mRNA-1
chr2_1	CDS	77837053	77837430	77837053	ID=Msa0227150-mRNA-1:cds;Parent=Msa0227150-mRNA-1
chr2_1	CDS	77837541	77837672	77837541	ID=Msa0227150-mRNA-1:cds;Parent=Msa0227150-mRNA-1

Gene Sequence

>Msa0227150

GAAATGGAAGAACATTTGTGTCACACAAACAGCACATTTGATGAAGAGTTCCTCAGAGATATCCTGTATCAGACTCCACAAGATCAATTCAGTGTCCCTATTGCCACAACTGGCCTAGTAAACAACTCATCCATCAATGTGTCACATTCAACTCAACATGCTGAAGAAAATCCAACCAACTCATTATCAATACCAACAATTGAACAACACCATGATTCTTTGCCTTTGTCATCATCAACGGATAACCAAGGGTCGAATTCAAAGAAGCCTCGAAGCGCTTCTGAGACATTAGATCACATAATGTCGGAGAGGAATAGGAGACAACTACTTACAAGTAAGATCATAGAACTTTCGGCCTTTATACCTGGATTGAAGAAGATTGATAAGGTTCATGTGGTAACGGAAGCTATCAATTACGTGAAACATCTTGAAGAACGTTTGAAAGAGCTAGAAGAAGACATTAAGAAGAAAGATGCAGGATCATTGAGCACCATAAATCTCGTGTTCTAA

Protein sequence

>Msa0227150

EMEEHLCHTNSTFDEEFLRDILYQTPQDQFSVPIATTGLVNNSSINVSHSTQHAEENPTNSLSIPTIEQHHDSLPLSSSTDNQGSNSKKPRSASETLDHIMSERNRRQLLTSKIIELSAFIPGLKKIDKVHVVTEAINYVKHLEERLKELEEDIKKKDAGSLSTINLVF*