Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0230190 | KEH24950.1 | 91.393 | 244 | 17 | 2 | 1 | 240 | 1 | 244 | 1.14e-161 | 458 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0230190 | sp|Q9STS6|LBD27_ARATH | 57.292 | 96 | 40 | 1 | 9 | 104 | 36 | 130 | 2.87e-31 | 120 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0230190 | A0A072UGP3 | 91.393 | 244 | 17 | 2 | 1 | 240 | 1 | 244 | 5.44e-162 | 458 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0230190 | TF | LOB |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0230190 | MtrunA17_Chr6g0452661 | 91.393 | 244 | 17 | 2 | 1 | 240 | 1 | 244 | 1.05e-165 | 458 |
| Msa0230190 | MtrunA17_Chr5g0439111 | 54.545 | 242 | 90 | 6 | 1 | 230 | 1 | 234 | 1.83e-64 | 201 |
| Msa0230190 | MtrunA17_Chr6g0452691 | 64.234 | 137 | 46 | 1 | 1 | 134 | 1 | 137 | 1.12e-58 | 184 |
| Msa0230190 | MtrunA17_Chr6g0452671 | 56.429 | 140 | 57 | 2 | 7 | 143 | 9 | 147 | 2.14e-49 | 161 |
| Msa0230190 | MtrunA17_Chr4g0055441 | 48.108 | 185 | 51 | 4 | 10 | 190 | 3 | 146 | 5.95e-47 | 153 |
| Msa0230190 | MtrunA17_Chr4g0059901 | 60.256 | 78 | 30 | 1 | 8 | 85 | 9 | 85 | 6.64e-29 | 110 |
| Msa0230190 | MtrunA17_Chr5g0402611 | 44.037 | 109 | 53 | 2 | 7 | 108 | 6 | 113 | 1.07e-28 | 110 |
| Msa0230190 | MtrunA17_Chr3g0115431 | 52.500 | 80 | 37 | 1 | 10 | 89 | 10 | 88 | 5.04e-28 | 106 |
| Msa0230190 | MtrunA17_Chr2g0311601 | 45.745 | 94 | 50 | 1 | 10 | 103 | 14 | 106 | 1.34e-27 | 103 |
| Msa0230190 | MtrunA17_Chr8g0342351 | 40.385 | 156 | 84 | 2 | 8 | 163 | 11 | 157 | 4.63e-27 | 105 |
| Msa0230190 | MtrunA17_Chr8g0353981 | 47.126 | 87 | 45 | 1 | 3 | 89 | 1 | 86 | 7.43e-27 | 103 |
| Msa0230190 | MtrunA17_Chr3g0106101 | 45.745 | 94 | 50 | 1 | 10 | 103 | 8 | 100 | 8.54e-27 | 102 |
| Msa0230190 | MtrunA17_Chr7g0227511 | 41.237 | 97 | 56 | 1 | 7 | 103 | 9 | 104 | 1.95e-26 | 101 |
| Msa0230190 | MtrunA17_Chr6g0449651 | 42.268 | 97 | 55 | 1 | 7 | 103 | 65 | 160 | 2.97e-26 | 101 |
| Msa0230190 | MtrunA17_Chr3g0117121 | 43.299 | 97 | 54 | 1 | 7 | 103 | 8 | 103 | 3.78e-26 | 100 |
| Msa0230190 | MtrunA17_Chr4g0058651 | 42.268 | 97 | 55 | 1 | 7 | 103 | 9 | 104 | 4.37e-26 | 100 |
| Msa0230190 | MtrunA17_Chr6g0449641 | 41.237 | 97 | 56 | 1 | 7 | 103 | 7 | 102 | 7.08e-26 | 99.0 |
| Msa0230190 | MtrunA17_Chr4g0030001 | 45.217 | 115 | 60 | 3 | 10 | 123 | 25 | 137 | 2.41e-25 | 99.0 |
| Msa0230190 | MtrunA17_Chr6g0449311 | 48.000 | 125 | 50 | 3 | 37 | 149 | 1 | 122 | 3.67e-25 | 97.1 |
| Msa0230190 | MtrunA17_Chr5g0437571 | 41.237 | 97 | 56 | 1 | 7 | 103 | 4 | 99 | 6.81e-25 | 97.1 |
| Msa0230190 | MtrunA17_Chr4g0043421 | 42.553 | 94 | 53 | 1 | 10 | 103 | 16 | 108 | 7.02e-25 | 97.1 |
| Msa0230190 | MtrunA17_Chr3g0114061 | 36.552 | 145 | 83 | 4 | 10 | 146 | 9 | 152 | 9.61e-25 | 96.3 |
| Msa0230190 | MtrunA17_Chr3g0097221 | 43.299 | 97 | 54 | 1 | 7 | 103 | 5 | 100 | 1.55e-24 | 99.4 |
| Msa0230190 | MtrunA17_Chr1g0200201 | 42.982 | 114 | 63 | 2 | 10 | 123 | 73 | 184 | 3.25e-24 | 97.1 |
| Msa0230190 | MtrunA17_Chr2g0330201 | 41.429 | 140 | 66 | 3 | 8 | 145 | 18 | 143 | 3.93e-24 | 98.2 |
| Msa0230190 | MtrunA17_Chr5g0432451 | 41.573 | 89 | 48 | 2 | 1 | 89 | 38 | 122 | 1.91e-22 | 92.4 |
| Msa0230190 | MtrunA17_Chr8g0374591 | 41.176 | 102 | 56 | 2 | 3 | 104 | 5 | 102 | 2.21e-22 | 89.4 |
| Msa0230190 | MtrunA17_Chr5g0438091 | 30.723 | 166 | 97 | 4 | 10 | 172 | 40 | 190 | 2.48e-22 | 91.3 |
| Msa0230190 | MtrunA17_Chr4g0040831 | 39.669 | 121 | 65 | 3 | 2 | 118 | 4 | 120 | 8.94e-22 | 89.7 |
| Msa0230190 | MtrunA17_Chr5g0437401 | 34.127 | 126 | 76 | 3 | 5 | 130 | 7 | 125 | 1.04e-21 | 89.0 |
| Msa0230190 | MtrunA17_Chr7g0225111 | 37.963 | 108 | 63 | 2 | 10 | 114 | 6 | 112 | 1.33e-21 | 87.0 |
| Msa0230190 | MtrunA17_Chr3g0129071 | 35.849 | 106 | 64 | 2 | 1 | 106 | 42 | 143 | 3.14e-21 | 89.4 |
| Msa0230190 | MtrunA17_Chr5g0444351 | 35.106 | 94 | 60 | 1 | 10 | 103 | 6 | 98 | 6.51e-21 | 85.9 |
| Msa0230190 | MtrunA17_Chr3g0113891 | 36.111 | 108 | 64 | 2 | 5 | 112 | 10 | 112 | 2.18e-20 | 85.5 |
| Msa0230190 | MtrunA17_Chr1g0184271 | 37.963 | 108 | 65 | 2 | 10 | 116 | 13 | 119 | 1.36e-18 | 82.0 |
| Msa0230190 | MtrunA17_Chr8g0374601 | 37.255 | 102 | 60 | 2 | 3 | 104 | 23 | 120 | 2.17e-18 | 82.4 |
| Msa0230190 | MtrunA17_Chr7g0260971 | 41.176 | 85 | 46 | 3 | 10 | 93 | 15 | 96 | 3.10e-17 | 77.8 |
| Msa0230190 | MtrunA17_Chr1g0184281 | 37.755 | 98 | 57 | 3 | 10 | 106 | 16 | 110 | 8.46e-17 | 76.6 |
| Msa0230190 | MtrunA17_Chr7g0245871 | 37.755 | 98 | 59 | 2 | 10 | 106 | 28 | 124 | 1.62e-16 | 76.6 |
| Msa0230190 | MtrunA17_Chr7g0261031 | 37.755 | 98 | 59 | 2 | 10 | 106 | 9 | 105 | 1.96e-16 | 75.9 |
| Msa0230190 | MtrunA17_Chr6g0460951 | 38.636 | 88 | 49 | 3 | 3 | 89 | 37 | 120 | 2.42e-16 | 75.9 |
| Msa0230190 | MtrunA17_Chr8g0374581 | 35.238 | 105 | 63 | 3 | 3 | 106 | 5 | 105 | 8.44e-16 | 74.3 |
| Msa0230190 | MtrunA17_Chr8g0351721 | 33.613 | 119 | 73 | 4 | 10 | 127 | 19 | 132 | 8.92e-16 | 73.9 |
| Msa0230190 | MtrunA17_Chr5g0435641 | 37.931 | 87 | 50 | 3 | 8 | 93 | 6 | 89 | 1.42e-15 | 72.8 |
| Msa0230190 | MtrunA17_Chr6g0460941 | 38.272 | 81 | 48 | 2 | 10 | 89 | 8 | 87 | 3.64e-15 | 69.3 |
| Msa0230190 | MtrunA17_Chr2g0326041 | 58.333 | 48 | 20 | 0 | 10 | 57 | 34 | 81 | 4.77e-15 | 68.6 |
| Msa0230190 | MtrunA17_Chr8g0351701 | 33.673 | 98 | 63 | 2 | 10 | 106 | 24 | 120 | 2.04e-14 | 70.5 |
| Msa0230190 | MtrunA17_Chr4g0046201 | 37.037 | 81 | 50 | 1 | 9 | 89 | 6 | 85 | 2.86e-14 | 70.5 |
| Msa0230190 | MtrunA17_Chr7g0245861 | 36.782 | 87 | 49 | 4 | 10 | 93 | 13 | 96 | 3.38e-12 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0230190 | AT3G47870.1 | 57.292 | 96 | 40 | 1 | 9 | 104 | 36 | 130 | 2.92e-32 | 120 |
| Msa0230190 | AT3G26660.1 | 43.750 | 96 | 53 | 1 | 8 | 103 | 4 | 98 | 3.65e-28 | 104 |
| Msa0230190 | AT3G26620.1 | 43.750 | 96 | 53 | 1 | 8 | 103 | 4 | 98 | 4.71e-28 | 104 |
| Msa0230190 | AT5G66870.1 | 35.762 | 151 | 90 | 3 | 10 | 154 | 8 | 157 | 3.58e-27 | 107 |
| Msa0230190 | AT3G13850.1 | 48.000 | 100 | 51 | 1 | 8 | 107 | 35 | 133 | 3.93e-27 | 105 |
| Msa0230190 | AT1G65620.1 | 50.602 | 83 | 40 | 1 | 7 | 89 | 7 | 88 | 7.03e-27 | 103 |
| Msa0230190 | AT1G65620.4 | 50.602 | 83 | 40 | 1 | 7 | 89 | 7 | 88 | 7.03e-27 | 103 |
| Msa0230190 | AT1G65620.3 | 50.602 | 83 | 40 | 1 | 7 | 89 | 7 | 88 | 7.03e-27 | 103 |
| Msa0230190 | AT1G65620.5 | 50.602 | 83 | 40 | 1 | 7 | 89 | 7 | 88 | 7.03e-27 | 103 |
| Msa0230190 | AT1G65620.2 | 50.602 | 83 | 40 | 1 | 7 | 89 | 7 | 88 | 7.03e-27 | 103 |
| Msa0230190 | AT3G27650.1 | 42.857 | 98 | 55 | 1 | 7 | 104 | 37 | 133 | 2.02e-26 | 101 |
| Msa0230190 | AT5G63090.4 | 42.268 | 97 | 55 | 1 | 7 | 103 | 9 | 104 | 1.76e-25 | 99.4 |
| Msa0230190 | AT5G63090.3 | 42.268 | 97 | 55 | 1 | 7 | 103 | 9 | 104 | 1.76e-25 | 99.4 |
| Msa0230190 | AT5G63090.2 | 42.268 | 97 | 55 | 1 | 7 | 103 | 9 | 104 | 1.76e-25 | 99.4 |
| Msa0230190 | AT5G63090.1 | 42.268 | 97 | 55 | 1 | 7 | 103 | 9 | 104 | 1.76e-25 | 99.4 |
| Msa0230190 | AT1G07900.1 | 45.745 | 94 | 50 | 1 | 10 | 103 | 34 | 126 | 4.37e-25 | 98.6 |
| Msa0230190 | AT2G23660.2 | 52.500 | 80 | 37 | 1 | 10 | 89 | 6 | 84 | 1.15e-24 | 100 |
| Msa0230190 | AT2G23660.3 | 52.500 | 80 | 37 | 1 | 10 | 89 | 6 | 84 | 1.15e-24 | 100 |
| Msa0230190 | AT2G23660.1 | 52.500 | 80 | 37 | 1 | 10 | 89 | 6 | 84 | 1.15e-24 | 100 |
| Msa0230190 | AT1G31320.1 | 48.750 | 80 | 40 | 1 | 10 | 89 | 14 | 92 | 1.44e-24 | 96.7 |
| Msa0230190 | AT2G28500.1 | 45.745 | 94 | 50 | 1 | 10 | 103 | 56 | 148 | 4.73e-24 | 97.1 |
| Msa0230190 | AT2G30130.1 | 41.489 | 94 | 54 | 1 | 10 | 103 | 9 | 101 | 1.79e-23 | 94.4 |
| Msa0230190 | AT3G50510.1 | 38.182 | 110 | 63 | 2 | 5 | 110 | 8 | 116 | 7.92e-22 | 90.1 |
| Msa0230190 | AT3G50510.2 | 38.182 | 110 | 63 | 2 | 5 | 110 | 8 | 116 | 7.92e-22 | 90.1 |
| Msa0230190 | AT2G40470.2 | 41.573 | 89 | 48 | 2 | 1 | 89 | 15 | 99 | 9.17e-22 | 90.1 |
| Msa0230190 | AT2G40470.1 | 41.573 | 89 | 48 | 2 | 1 | 89 | 40 | 124 | 2.00e-21 | 89.7 |
| Msa0230190 | AT1G16530.1 | 39.000 | 100 | 59 | 2 | 10 | 108 | 15 | 113 | 4.15e-21 | 87.4 |
| Msa0230190 | AT2G30340.2 | 41.573 | 89 | 48 | 2 | 1 | 89 | 48 | 132 | 5.12e-21 | 89.7 |
| Msa0230190 | AT2G30340.1 | 41.573 | 89 | 48 | 2 | 1 | 89 | 47 | 131 | 5.24e-21 | 89.7 |
| Msa0230190 | AT3G11090.1 | 38.824 | 85 | 51 | 1 | 5 | 89 | 7 | 90 | 1.76e-20 | 85.9 |
| Msa0230190 | AT1G72980.1 | 39.167 | 120 | 64 | 3 | 9 | 121 | 13 | 130 | 3.44e-20 | 86.3 |
| Msa0230190 | AT1G06280.1 | 44.444 | 81 | 44 | 1 | 7 | 87 | 22 | 101 | 3.57e-19 | 83.2 |
| Msa0230190 | AT5G15060.1 | 47.945 | 73 | 37 | 1 | 10 | 81 | 18 | 90 | 3.98e-18 | 79.0 |
| Msa0230190 | AT3G58190.1 | 35.915 | 142 | 80 | 7 | 10 | 148 | 12 | 145 | 1.01e-16 | 77.0 |
| Msa0230190 | AT2G42430.1 | 40.000 | 85 | 47 | 3 | 10 | 93 | 16 | 97 | 3.72e-16 | 75.9 |
| Msa0230190 | AT3G03760.1 | 38.272 | 81 | 48 | 2 | 10 | 89 | 52 | 131 | 8.69e-16 | 75.5 |
| Msa0230190 | AT4G00220.1 | 35.354 | 99 | 62 | 2 | 12 | 109 | 20 | 117 | 1.40e-15 | 73.9 |
| Msa0230190 | AT4G00210.1 | 35.922 | 103 | 60 | 4 | 10 | 111 | 12 | 109 | 4.12e-15 | 72.4 |
| Msa0230190 | AT2G42440.1 | 36.735 | 98 | 60 | 2 | 10 | 106 | 8 | 104 | 4.29e-15 | 72.8 |
| Msa0230190 | AT5G06080.1 | 39.535 | 86 | 48 | 3 | 9 | 93 | 7 | 89 | 5.29e-15 | 71.2 |
| Msa0230190 | AT2G31310.1 | 39.506 | 81 | 47 | 2 | 10 | 89 | 8 | 87 | 2.00e-14 | 70.1 |
| Msa0230190 | AT2G45420.1 | 39.759 | 83 | 46 | 3 | 12 | 93 | 40 | 119 | 3.68e-14 | 70.5 |
| Msa0230190 | AT5G35900.1 | 32.979 | 94 | 62 | 1 | 10 | 103 | 6 | 98 | 1.65e-13 | 67.8 |
| Msa0230190 | AT2G19820.1 | 37.037 | 81 | 51 | 0 | 10 | 90 | 13 | 93 | 6.22e-13 | 64.3 |
| Msa0230190 | AT2G45410.1 | 36.145 | 83 | 49 | 3 | 12 | 93 | 19 | 98 | 7.56e-12 | 63.2 |
Find 51 sgRNAs with CRISPR-Local
Find 102 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCAAGTGTTTAACAATTA+TGG | 0.193339 | 2_2:-2096726 | Msa0230190:CDS |
| CGTGTAAATTTCCCAATTCA+TGG | 0.260696 | 2_2:-2096885 | Msa0230190:CDS |
| TGAGCATCGCCGAACATCTT+TGG | 0.279625 | 2_2:+2097011 | None:intergenic |
| GGTTTATCGGCCGGAAAATA+AGG | 0.293537 | 2_2:+2097032 | None:intergenic |
| GCCGAACATCTTTGGTTTAT+CGG | 0.298825 | 2_2:+2097019 | None:intergenic |
| GAAGATCCTAAACGAAATTA+AGG | 0.309552 | 2_2:-2096961 | Msa0230190:CDS |
| CATGCTGCACAAGCCTTGTT+TGG | 0.313109 | 2_2:+2097098 | None:intergenic |
| AGTCTTCTAAACAATTAAAA+TGG | 0.313955 | 2_2:-2096821 | Msa0230190:CDS |
| AAACTTGAATATAAATTCTA+TGG | 0.323327 | 2_2:-2096595 | Msa0230190:CDS |
| ACACCAAGACATCCATGAAT+TGG | 0.323416 | 2_2:+2096873 | None:intergenic |
| CACCATCCTTAATTTCGTTT+AGG | 0.338574 | 2_2:+2096955 | None:intergenic |
| GGCGATGCTCATCGTTTGTT+TGG | 0.378693 | 2_2:-2096999 | Msa0230190:CDS |
| AGAATTGGACAGTGAAGAAT+TGG | 0.385906 | 2_2:-2096514 | Msa0230190:CDS |
| TTTGATTGGGAGGAAATAGA+AGG | 0.386548 | 2_2:-2096462 | Msa0230190:CDS |
| TTGAAAACTAGTAGAAGGTT+TGG | 0.389422 | 2_2:+2096743 | None:intergenic |
| AACACTTGAAAACTAGTAGA+AGG | 0.390895 | 2_2:+2096738 | None:intergenic |
| AAATTAGAACAATATTTGAT+TGG | 0.395330 | 2_2:-2096476 | Msa0230190:CDS |
| GGACAATGTCAAATTAGAAT+TGG | 0.404194 | 2_2:-2096529 | Msa0230190:CDS |
| TTCCCAATTCATGGATGTCT+TGG | 0.407544 | 2_2:-2096876 | Msa0230190:CDS |
| GTTTGGTTTGATGATATTCA+TGG | 0.411799 | 2_2:+2097115 | None:intergenic |
| AATTAGAACAATATTTGATT+GGG | 0.439912 | 2_2:-2096475 | Msa0230190:CDS |
| CACCAAGACATCCATGAATT+GGG | 0.452237 | 2_2:+2096874 | None:intergenic |
| TACTGATGAACTACCTAGTT+CGG | 0.470461 | 2_2:-2096430 | Msa0230190:CDS |
| GGTGTTATTCATATGTATGC+AGG | 0.474469 | 2_2:-2096855 | Msa0230190:CDS |
| TTTAACAATTATGGTGATGT+TGG | 0.489657 | 2_2:-2096717 | Msa0230190:CDS |
| GCCGATAAACCAAAGATGTT+CGG | 0.490468 | 2_2:-2097020 | Msa0230190:CDS |
| AACATCTTTGGTTTATCGGC+CGG | 0.491014 | 2_2:+2097023 | None:intergenic |
| TAGAACAATATTTGATTGGG+AGG | 0.497294 | 2_2:-2096472 | Msa0230190:CDS |
| AATTTGACATTGTCCCCACC+AGG | 0.497390 | 2_2:+2096537 | None:intergenic |
| AAAACTAGTAGAAGGTTTGG+AGG | 0.499480 | 2_2:+2096746 | None:intergenic |
| ATATAAATTCTATGGATACA+AGG | 0.500818 | 2_2:-2096587 | Msa0230190:CDS |
| GAAGAATTATTGACATCATG+AGG | 0.508817 | 2_2:+2096627 | None:intergenic |
| TTTGGTTTGATGATATTCAT+GGG | 0.513678 | 2_2:+2097116 | None:intergenic |
| CCCACGTTCATCTATAGCAC+TGG | 0.520231 | 2_2:+2096386 | None:intergenic |
| GCTATAGATGAACGTGGGAA+AGG | 0.547005 | 2_2:-2096381 | Msa0230190:CDS |
| TCACTAACATAAGAAGAAGA+TGG | 0.561786 | 2_2:+2096657 | None:intergenic |
| TATAAATTCTATGGATACAA+GGG | 0.572771 | 2_2:-2096586 | Msa0230190:CDS |
| AAGAATTATTGACATCATGA+GGG | 0.573919 | 2_2:+2096628 | None:intergenic |
| TCTATATTAAAATCCGAACT+AGG | 0.583710 | 2_2:+2096417 | None:intergenic |
| ATCCTAAACGAAATTAAGGA+TGG | 0.593456 | 2_2:-2096957 | Msa0230190:CDS |
| ATTATTGACATCATGAGGGG+TGG | 0.594299 | 2_2:+2096632 | None:intergenic |
| GAATATCATCAAACCAAACA+AGG | 0.597163 | 2_2:-2097111 | Msa0230190:CDS |
| GTGGTGATAGCAACCCTGGT+GGG | 0.642025 | 2_2:-2096551 | Msa0230190:CDS |
| TTGAGTGGTGATAGCAACCC+TGG | 0.645703 | 2_2:-2096555 | Msa0230190:CDS |
| ATTTGACATTGTCCCCACCA+GGG | 0.654875 | 2_2:+2096538 | None:intergenic |
| ACAAGGGTTGCAAAATTGAG+TGG | 0.655912 | 2_2:-2096570 | Msa0230190:CDS |
| AGAATTATTGACATCATGAG+GGG | 0.666482 | 2_2:+2096629 | None:intergenic |
| TCCAGTGCTATAGATGAACG+TGG | 0.671304 | 2_2:-2096387 | Msa0230190:CDS |
| TGGTGATAGCAACCCTGGTG+GGG | 0.705436 | 2_2:-2096550 | Msa0230190:CDS |
| CCAGTGCTATAGATGAACGT+GGG | 0.709401 | 2_2:-2096386 | Msa0230190:CDS |
| AGTGGTGATAGCAACCCTGG+TGG | 0.759892 | 2_2:-2096552 | Msa0230190:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATATTTTGTTGTTGTAAA+TGG | + | chr2_2:2096715-2096734 | None:intergenic | 15.0% |
| !! | AAACTTGAATATAAATTCTA+TGG | - | chr2_2:2096889-2096908 | Msa0230190:CDS | 15.0% |
| !! | AAATTAGAACAATATTTGAT+TGG | - | chr2_2:2097008-2097027 | Msa0230190:CDS | 15.0% |
| !! | AATTAGAACAATATTTGATT+GGG | - | chr2_2:2097009-2097028 | Msa0230190:CDS | 15.0% |
| !!! | AAATATTTTGTTGTTGTAAA+TGG | + | chr2_2:2096715-2096734 | None:intergenic | 15.0% |
| !! | AAACTTGAATATAAATTCTA+TGG | - | chr2_2:2096889-2096908 | Msa0230190:CDS | 15.0% |
| !! | AAATTAGAACAATATTTGAT+TGG | - | chr2_2:2097008-2097027 | Msa0230190:CDS | 15.0% |
| !! | AATTAGAACAATATTTGATT+GGG | - | chr2_2:2097009-2097028 | Msa0230190:CDS | 15.0% |
| !! | AGTCTTCTAAACAATTAAAA+TGG | - | chr2_2:2096663-2096682 | Msa0230190:CDS | 20.0% |
| !!! | TTTCAAGTGTTTAACAATTA+TGG | - | chr2_2:2096758-2096777 | Msa0230190:CDS | 20.0% |
| !! | ATATAAATTCTATGGATACA+AGG | - | chr2_2:2096897-2096916 | Msa0230190:CDS | 20.0% |
| !! | TATAAATTCTATGGATACAA+GGG | - | chr2_2:2096898-2096917 | Msa0230190:CDS | 20.0% |
| !! | AGTCTTCTAAACAATTAAAA+TGG | - | chr2_2:2096663-2096682 | Msa0230190:CDS | 20.0% |
| !!! | TTTCAAGTGTTTAACAATTA+TGG | - | chr2_2:2096758-2096777 | Msa0230190:CDS | 20.0% |
| !! | ATATAAATTCTATGGATACA+AGG | - | chr2_2:2096897-2096916 | Msa0230190:CDS | 20.0% |
| !! | TATAAATTCTATGGATACAA+GGG | - | chr2_2:2096898-2096917 | Msa0230190:CDS | 20.0% |
| !! | TTTAACAATTATGGTGATGT+TGG | - | chr2_2:2096767-2096786 | Msa0230190:CDS | 25.0% |
| ! | AAGAATTATTGACATCATGA+GGG | + | chr2_2:2096859-2096878 | None:intergenic | 25.0% |
| ! | TCTATATTAAAATCCGAACT+AGG | + | chr2_2:2097070-2097089 | None:intergenic | 25.0% |
| !! | TTTAACAATTATGGTGATGT+TGG | - | chr2_2:2096767-2096786 | Msa0230190:CDS | 25.0% |
| ! | AAGAATTATTGACATCATGA+GGG | + | chr2_2:2096859-2096878 | None:intergenic | 25.0% |
| ! | TCTATATTAAAATCCGAACT+AGG | + | chr2_2:2097070-2097089 | None:intergenic | 25.0% |
| GAATATCATCAAACCAAACA+AGG | - | chr2_2:2096373-2096392 | Msa0230190:CDS | 30.0% | |
| !!! | TGTTTTAGCTCCTTATTTTC+CGG | - | chr2_2:2096442-2096461 | Msa0230190:CDS | 30.0% |
| GAAGATCCTAAACGAAATTA+AGG | - | chr2_2:2096523-2096542 | Msa0230190:CDS | 30.0% | |
| ATCCTAAACGAAATTAAGGA+TGG | - | chr2_2:2096527-2096546 | Msa0230190:CDS | 30.0% | |
| TTGAAAACTAGTAGAAGGTT+TGG | + | chr2_2:2096744-2096763 | None:intergenic | 30.0% | |
| AACACTTGAAAACTAGTAGA+AGG | + | chr2_2:2096749-2096768 | None:intergenic | 30.0% | |
| TCACTAACATAAGAAGAAGA+TGG | + | chr2_2:2096830-2096849 | None:intergenic | 30.0% | |
| AGAATTATTGACATCATGAG+GGG | + | chr2_2:2096858-2096877 | None:intergenic | 30.0% | |
| GAAGAATTATTGACATCATG+AGG | + | chr2_2:2096860-2096879 | None:intergenic | 30.0% | |
| GGACAATGTCAAATTAGAAT+TGG | - | chr2_2:2096955-2096974 | Msa0230190:CDS | 30.0% | |
| TAGAACAATATTTGATTGGG+AGG | - | chr2_2:2097012-2097031 | Msa0230190:CDS | 30.0% | |
| GAATATCATCAAACCAAACA+AGG | - | chr2_2:2096373-2096392 | Msa0230190:CDS | 30.0% | |
| !!! | TGTTTTAGCTCCTTATTTTC+CGG | - | chr2_2:2096442-2096461 | Msa0230190:CDS | 30.0% |
| GAAGATCCTAAACGAAATTA+AGG | - | chr2_2:2096523-2096542 | Msa0230190:CDS | 30.0% | |
| ATCCTAAACGAAATTAAGGA+TGG | - | chr2_2:2096527-2096546 | Msa0230190:CDS | 30.0% | |
| TTGAAAACTAGTAGAAGGTT+TGG | + | chr2_2:2096744-2096763 | None:intergenic | 30.0% | |
| AACACTTGAAAACTAGTAGA+AGG | + | chr2_2:2096749-2096768 | None:intergenic | 30.0% | |
| TCACTAACATAAGAAGAAGA+TGG | + | chr2_2:2096830-2096849 | None:intergenic | 30.0% | |
| AGAATTATTGACATCATGAG+GGG | + | chr2_2:2096858-2096877 | None:intergenic | 30.0% | |
| GAAGAATTATTGACATCATG+AGG | + | chr2_2:2096860-2096879 | None:intergenic | 30.0% | |
| GGACAATGTCAAATTAGAAT+TGG | - | chr2_2:2096955-2096974 | Msa0230190:CDS | 30.0% | |
| TAGAACAATATTTGATTGGG+AGG | - | chr2_2:2097012-2097031 | Msa0230190:CDS | 30.0% | |
| CACCATCCTTAATTTCGTTT+AGG | + | chr2_2:2096532-2096551 | None:intergenic | 35.0% | |
| CGTGTAAATTTCCCAATTCA+TGG | - | chr2_2:2096599-2096618 | Msa0230190:CDS | 35.0% | |
| !! | GGTGTTATTCATATGTATGC+AGG | - | chr2_2:2096629-2096648 | Msa0230190:CDS | 35.0% |
| AAAACTAGTAGAAGGTTTGG+AGG | + | chr2_2:2096741-2096760 | None:intergenic | 35.0% | |
| AGAATTGGACAGTGAAGAAT+TGG | - | chr2_2:2096970-2096989 | Msa0230190:CDS | 35.0% | |
| TTTGATTGGGAGGAAATAGA+AGG | - | chr2_2:2097022-2097041 | Msa0230190:CDS | 35.0% | |
| TACTGATGAACTACCTAGTT+CGG | - | chr2_2:2097054-2097073 | Msa0230190:CDS | 35.0% | |
| CACCATCCTTAATTTCGTTT+AGG | + | chr2_2:2096532-2096551 | None:intergenic | 35.0% | |
| CGTGTAAATTTCCCAATTCA+TGG | - | chr2_2:2096599-2096618 | Msa0230190:CDS | 35.0% | |
| !! | GGTGTTATTCATATGTATGC+AGG | - | chr2_2:2096629-2096648 | Msa0230190:CDS | 35.0% |
| AAAACTAGTAGAAGGTTTGG+AGG | + | chr2_2:2096741-2096760 | None:intergenic | 35.0% | |
| AGAATTGGACAGTGAAGAAT+TGG | - | chr2_2:2096970-2096989 | Msa0230190:CDS | 35.0% | |
| TTTGATTGGGAGGAAATAGA+AGG | - | chr2_2:2097022-2097041 | Msa0230190:CDS | 35.0% | |
| TACTGATGAACTACCTAGTT+CGG | - | chr2_2:2097054-2097073 | Msa0230190:CDS | 35.0% | |
| ! | AACATCTTTGGTTTATCGGC+CGG | + | chr2_2:2096464-2096483 | None:intergenic | 40.0% |
| GCCGATAAACCAAAGATGTT+CGG | - | chr2_2:2096464-2096483 | Msa0230190:CDS | 40.0% | |
| ! | GCCGAACATCTTTGGTTTAT+CGG | + | chr2_2:2096468-2096487 | None:intergenic | 40.0% |
| TTCCCAATTCATGGATGTCT+TGG | - | chr2_2:2096608-2096627 | Msa0230190:CDS | 40.0% | |
| CACCAAGACATCCATGAATT+GGG | + | chr2_2:2096613-2096632 | None:intergenic | 40.0% | |
| ACACCAAGACATCCATGAAT+TGG | + | chr2_2:2096614-2096633 | None:intergenic | 40.0% | |
| ATTATTGACATCATGAGGGG+TGG | + | chr2_2:2096855-2096874 | None:intergenic | 40.0% | |
| ACAAGGGTTGCAAAATTGAG+TGG | - | chr2_2:2096914-2096933 | Msa0230190:CDS | 40.0% | |
| ! | AACATCTTTGGTTTATCGGC+CGG | + | chr2_2:2096464-2096483 | None:intergenic | 40.0% |
| GCCGATAAACCAAAGATGTT+CGG | - | chr2_2:2096464-2096483 | Msa0230190:CDS | 40.0% | |
| ! | GCCGAACATCTTTGGTTTAT+CGG | + | chr2_2:2096468-2096487 | None:intergenic | 40.0% |
| TTCCCAATTCATGGATGTCT+TGG | - | chr2_2:2096608-2096627 | Msa0230190:CDS | 40.0% | |
| CACCAAGACATCCATGAATT+GGG | + | chr2_2:2096613-2096632 | None:intergenic | 40.0% | |
| ACACCAAGACATCCATGAAT+TGG | + | chr2_2:2096614-2096633 | None:intergenic | 40.0% | |
| ATTATTGACATCATGAGGGG+TGG | + | chr2_2:2096855-2096874 | None:intergenic | 40.0% | |
| ACAAGGGTTGCAAAATTGAG+TGG | - | chr2_2:2096914-2096933 | Msa0230190:CDS | 40.0% | |
| GGTTTATCGGCCGGAAAATA+AGG | + | chr2_2:2096455-2096474 | None:intergenic | 45.0% | |
| ATTTGACATTGTCCCCACCA+GGG | + | chr2_2:2096949-2096968 | None:intergenic | 45.0% | |
| AATTTGACATTGTCCCCACC+AGG | + | chr2_2:2096950-2096969 | None:intergenic | 45.0% | |
| TCCAGTGCTATAGATGAACG+TGG | - | chr2_2:2097097-2097116 | Msa0230190:CDS | 45.0% | |
| CCAGTGCTATAGATGAACGT+GGG | - | chr2_2:2097098-2097117 | Msa0230190:CDS | 45.0% | |
| GCTATAGATGAACGTGGGAA+AGG | - | chr2_2:2097103-2097122 | Msa0230190:CDS | 45.0% | |
| GGTTTATCGGCCGGAAAATA+AGG | + | chr2_2:2096455-2096474 | None:intergenic | 45.0% | |
| ATTTGACATTGTCCCCACCA+GGG | + | chr2_2:2096949-2096968 | None:intergenic | 45.0% | |
| AATTTGACATTGTCCCCACC+AGG | + | chr2_2:2096950-2096969 | None:intergenic | 45.0% | |
| TCCAGTGCTATAGATGAACG+TGG | - | chr2_2:2097097-2097116 | Msa0230190:CDS | 45.0% | |
| CCAGTGCTATAGATGAACGT+GGG | - | chr2_2:2097098-2097117 | Msa0230190:CDS | 45.0% | |
| GCTATAGATGAACGTGGGAA+AGG | - | chr2_2:2097103-2097122 | Msa0230190:CDS | 45.0% | |
| !! | CATGCTGCACAAGCCTTGTT+TGG | + | chr2_2:2096389-2096408 | None:intergenic | 50.0% |
| ! | TGAGCATCGCCGAACATCTT+TGG | + | chr2_2:2096476-2096495 | None:intergenic | 50.0% |
| GGCGATGCTCATCGTTTGTT+TGG | - | chr2_2:2096485-2096504 | Msa0230190:CDS | 50.0% | |
| TTGAGTGGTGATAGCAACCC+TGG | - | chr2_2:2096929-2096948 | Msa0230190:CDS | 50.0% | |
| CCCACGTTCATCTATAGCAC+TGG | + | chr2_2:2097101-2097120 | None:intergenic | 50.0% | |
| !! | CATGCTGCACAAGCCTTGTT+TGG | + | chr2_2:2096389-2096408 | None:intergenic | 50.0% |
| ! | TGAGCATCGCCGAACATCTT+TGG | + | chr2_2:2096476-2096495 | None:intergenic | 50.0% |
| GGCGATGCTCATCGTTTGTT+TGG | - | chr2_2:2096485-2096504 | Msa0230190:CDS | 50.0% | |
| TTGAGTGGTGATAGCAACCC+TGG | - | chr2_2:2096929-2096948 | Msa0230190:CDS | 50.0% | |
| CCCACGTTCATCTATAGCAC+TGG | + | chr2_2:2097101-2097120 | None:intergenic | 50.0% | |
| AGTGGTGATAGCAACCCTGG+TGG | - | chr2_2:2096932-2096951 | Msa0230190:CDS | 55.0% | |
| GTGGTGATAGCAACCCTGGT+GGG | - | chr2_2:2096933-2096952 | Msa0230190:CDS | 55.0% | |
| TGGTGATAGCAACCCTGGTG+GGG | - | chr2_2:2096934-2096953 | Msa0230190:CDS | 55.0% | |
| AGTGGTGATAGCAACCCTGG+TGG | - | chr2_2:2096932-2096951 | Msa0230190:CDS | 55.0% | |
| GTGGTGATAGCAACCCTGGT+GGG | - | chr2_2:2096933-2096952 | Msa0230190:CDS | 55.0% | |
| TGGTGATAGCAACCCTGGTG+GGG | - | chr2_2:2096934-2096953 | Msa0230190:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_2 | gene | 2096371 | 2097135 | 2096371 | ID=Msa0230190;Name=Msa0230190 |
| chr2_2 | mRNA | 2096371 | 2097135 | 2096371 | ID=Msa0230190-mRNA-1;Parent=Msa0230190;Name=Msa0230190-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|-1|0|1|-1|1|1|0|254 |
| chr2_2 | exon | 2096371 | 2097135 | 2096371 | ID=Msa0230190-mRNA-1:exon:799;Parent=Msa0230190-mRNA-1 |
| chr2_2 | CDS | 2096371 | 2097135 | 2096371 | ID=Msa0230190-mRNA-1:cds;Parent=Msa0230190-mRNA-1 |
| Gene Sequence |
| Protein sequence |
