AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0230250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0230250 MtrunA17_Chr6g0470151 46.791 374 164 10 1 360 1 353 3.39e-62 207
Msa0230250 MtrunA17_Chr2g0293901 77.586 174 35 2 1 174 1 170 1.06e-61 200
Msa0230250 MtrunA17_Chr2g0289181 43.777 233 51 3 324 476 1 233 2.44e-55 184
Msa0230250 MtrunA17_Chr8g0366921 46.386 332 152 9 1 318 1 320 3.44e-55 187
Msa0230250 MtrunA17_Chr7g0266621 47.962 319 146 5 1 316 1 302 4.53e-47 165
Msa0230250 MtrunA17_Chr8g0368721 51.402 321 144 7 1 318 1 312 2.47e-43 155
Msa0230250 MtrunA17_Chr1g0202461 47.706 109 54 3 303 411 229 334 2.80e-25 107
Msa0230250 MtrunA17_Chr8g0348311 47.959 98 48 2 314 411 134 228 1.43e-22 98.6
Msa0230250 MtrunA17_Chr7g0248691 47.059 102 51 2 312 413 131 229 8.31e-22 96.3
Msa0230250 MtrunA17_Chr6g0470161 55.072 69 31 0 408 476 52 120 1.51e-20 87.0
Msa0230250 MtrunA17_Chr2g0293481 39.669 121 61 3 302 411 221 340 6.71e-20 92.4
Msa0230250 MtrunA17_Chr4g0053281 42.593 108 55 1 294 394 216 323 1.79e-19 89.4
Msa0230250 MtrunA17_Chr4g0071521 45.783 83 42 1 315 394 220 302 1.87e-19 89.0
Msa0230250 MtrunA17_Chr8g0387501 40.351 114 66 2 338 451 130 241 2.46e-19 87.8
Msa0230250 MtrunA17_Chr8g0379461 46.429 84 38 1 337 420 76 152 5.43e-19 86.3
Msa0230250 MtrunA17_Chr5g0401821 45.783 83 42 1 315 394 174 256 6.17e-19 86.7
Msa0230250 MtrunA17_Chr7g0270051 40.171 117 57 2 288 397 3 113 9.62e-19 85.9
Msa0230250 MtrunA17_Chr2g0319961 57.377 61 26 0 337 397 80 140 4.17e-18 84.0
Msa0230250 MtrunA17_Chr2g0284321 55.738 61 27 0 337 397 35 95 4.80e-18 82.8
Msa0230250 MtrunA17_Chr1g0184691 57.627 59 25 0 338 396 294 352 8.53e-18 84.7
Msa0230250 MtrunA17_Chr7g0228941 50.769 65 32 0 331 395 46 110 2.12e-17 78.6
Msa0230250 MtrunA17_Chr1g0147881 52.459 61 29 0 334 394 259 319 4.99e-17 82.4
Msa0230250 MtrunA17_Chr3g0139021 40.230 87 51 1 309 394 285 371 1.16e-16 81.6
Msa0230250 MtrunA17_Chr4g0074001 46.250 80 41 2 319 397 340 418 1.58e-16 82.0
Msa0230250 MtrunA17_Chr7g0263531 52.632 76 34 2 333 408 185 258 6.74e-16 79.7
Msa0230250 MtrunA17_Chr7g0263531 42.424 66 37 1 332 397 356 420 7.08e-14 73.6
Msa0230250 MtrunA17_Chr7g0218081 50.000 66 32 1 332 397 405 469 2.60e-15 78.2
Msa0230250 MtrunA17_Chr7g0218081 46.970 66 33 2 333 398 224 287 3.99e-13 71.6
Msa0230250 MtrunA17_Chr5g0445311 43.056 72 40 1 326 397 353 423 7.27e-15 77.0
Msa0230250 MtrunA17_Chr5g0445311 44.444 72 38 2 336 407 199 268 4.43e-12 68.2
Msa0230250 MtrunA17_Chr3g0079691 42.391 92 47 4 336 425 279 366 2.07e-14 75.9
Msa0230250 MtrunA17_Chr3g0079691 35.354 99 52 2 310 397 457 554 4.01e-12 68.6
Msa0230250 MtrunA17_Chr3g0101131 41.667 72 41 1 326 397 404 474 3.23e-14 75.1
Msa0230250 MtrunA17_Chr3g0101131 45.946 74 38 2 333 406 239 310 5.01e-13 71.2
Msa0230250 MtrunA17_Chr2g0302391 44.615 65 36 0 337 401 211 275 5.52e-14 73.9
Msa0230250 MtrunA17_Chr5g0447701 48.485 66 33 1 332 397 247 311 6.04e-14 73.6
Msa0230250 MtrunA17_Chr3g0099641 31.387 137 83 2 278 414 203 328 1.06e-13 73.2
Msa0230250 MtrunA17_Chr5g0418191 45.455 66 35 1 332 397 315 379 1.18e-13 72.8
Msa0230250 MtrunA17_Chr5g0418191 49.231 65 31 2 333 397 147 209 1.09e-11 66.6
Msa0230250 MtrunA17_Chr3g0136961 45.614 57 31 0 338 394 139 195 1.69e-13 71.2
Msa0230250 MtrunA17_Chr5g0432161 48.333 60 30 1 338 397 422 480 2.31e-13 72.4
Msa0230250 MtrunA17_Chr5g0432161 52.381 63 28 2 336 398 243 303 2.03e-12 69.3
Msa0230250 MtrunA17_Chr2g0322391 36.036 111 66 2 304 414 321 426 2.46e-13 72.4
Msa0230250 MtrunA17_Chr4g0075201 27.429 175 108 4 239 397 156 327 2.67e-13 72.0
Msa0230250 MtrunA17_Chr7g0270191 36.170 94 56 3 307 397 112 204 4.80e-13 69.7
Msa0230250 MtrunA17_Chr4g0001361 42.188 64 37 0 338 401 163 226 5.86e-13 69.7
Msa0230250 MtrunA17_Chr7g0249311 30.435 138 78 6 281 402 321 456 7.31e-13 70.9
Msa0230250 MtrunA17_Chr7g0249311 44.000 75 36 3 336 408 178 248 5.43e-11 65.1
Msa0230250 MtrunA17_Chr1g0194481 36.667 90 55 2 317 406 139 226 7.53e-13 69.3
Msa0230250 MtrunA17_Chr7g0238921 40.698 86 45 3 324 409 113 192 7.72e-13 69.3
Msa0230250 MtrunA17_Chr7g0270831 38.462 78 47 1 320 397 200 276 9.09e-13 70.1
Msa0230250 MtrunA17_Chr3g0106491 46.667 60 31 1 338 397 366 424 1.01e-12 70.1
Msa0230250 MtrunA17_Chr3g0106491 51.613 62 28 2 336 397 197 256 4.57e-12 68.2
Msa0230250 MtrunA17_Chr4g0042831 43.750 64 35 1 334 397 275 337 1.23e-12 69.3
Msa0230250 MtrunA17_Chr4g0042831 33.010 103 61 2 299 401 114 208 7.65e-11 63.9
Msa0230250 MtrunA17_Chr4g0025061 36.145 83 53 0 332 414 355 437 1.41e-12 70.1
Msa0230250 MtrunA17_Chr1g0150271 27.778 162 100 4 243 399 14 163 1.71e-12 67.4
Msa0230250 MtrunA17_Chr2g0288871 35.000 100 61 3 299 397 269 365 1.94e-12 69.3
Msa0230250 MtrunA17_Chr7g0249301 47.541 61 29 3 338 397 293 351 3.52e-12 67.8
Msa0230250 MtrunA17_Chr7g0266611 63.077 65 23 1 412 476 102 165 4.18e-12 64.3
Msa0230250 MtrunA17_Chr4g0025051 36.145 83 53 0 332 414 330 412 4.38e-12 68.2
Msa0230250 MtrunA17_Chr7g0243891 47.143 70 35 2 329 398 208 275 6.58e-12 67.8
Msa0230250 MtrunA17_Chr1g0150281 43.860 57 32 0 338 394 141 197 6.59e-12 66.2
Msa0230250 MtrunA17_Chr4g0004911 42.424 66 37 1 332 397 331 395 8.36e-12 67.4
Msa0230250 MtrunA17_Chr3g0129321 40.625 64 37 1 334 397 115 177 1.87e-11 63.5
Msa0230250 MtrunA17_Chr1g0150801 44.262 61 33 1 334 394 99 158 2.28e-11 62.4
Msa0230250 MtrunA17_Chr2g0294231 45.000 60 32 1 338 397 204 262 2.42e-11 65.5
Msa0230250 MtrunA17_Chr4g0060271 38.462 78 46 1 322 397 238 315 2.48e-11 65.9
Msa0230250 MtrunA17_Chr2g0333851 37.805 82 47 1 322 399 150 231 2.93e-11 64.7
Msa0230250 MtrunA17_Chr4g0051881 41.429 70 39 2 333 402 175 242 2.97e-11 64.7
Msa0230250 MtrunA17_Chr7g0216891 31.858 113 72 3 282 394 169 276 4.96e-11 65.1
Msa0230250 MtrunA17_Chr5g0443131 41.538 65 37 1 338 402 158 221 5.05e-11 63.9
Msa0230250 MtrunA17_Chr4g0068541 37.681 69 42 1 338 406 209 276 6.33e-11 64.3
Msa0230250 MtrunA17_Chr5g0418781 35.000 100 63 2 295 394 83 180 7.13e-11 62.8
Msa0230250 MtrunA17_Chr4g0051181 41.667 60 35 0 338 397 244 303 9.01e-11 63.9
Msa0230250 MtrunA17_Chr1g0188941 32.184 87 54 2 311 397 29 110 9.37e-11 58.9
Msa0230250 MtrunA17_Chr8g0383251 37.349 83 49 2 317 397 86 167 9.75e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0230250 AT5G52830.1 51.020 98 46 2 314 411 143 238 4.52e-25 106
Msa0230250 AT4G01250.1 32.850 207 126 6 277 475 72 273 2.42e-24 103
Msa0230250 AT2G34830.1 42.308 104 49 3 312 406 182 283 1.18e-18 88.6
Msa0230250 AT2G34830.2 42.308 104 49 3 312 406 191 292 1.44e-18 88.2
Msa0230250 AT5G45050.4 44.681 94 50 2 314 406 1017 1109 1.97e-18 89.4
Msa0230250 AT5G45050.1 44.681 94 50 2 314 406 1156 1248 2.15e-18 89.4
Msa0230250 AT2G24570.1 53.968 63 29 0 334 396 240 302 2.33e-18 86.3
Msa0230250 AT5G45050.2 44.681 94 50 2 314 406 1128 1220 2.33e-18 89.0
Msa0230250 AT4G23550.1 41.758 91 52 1 321 411 124 213 2.39e-18 86.3
Msa0230250 AT2G23320.1 57.895 57 24 0 338 394 241 297 2.85e-18 85.9
Msa0230250 AT4G31550.2 55.738 61 27 0 334 394 242 302 2.86e-18 86.3
Msa0230250 AT4G31550.1 55.738 61 27 0 334 394 243 303 2.87e-18 86.3
Msa0230250 AT5G28650.1 47.059 85 42 1 315 396 237 321 3.71e-18 85.9
Msa0230250 AT1G30650.1 42.308 104 49 3 312 406 184 285 3.86e-18 87.0
Msa0230250 AT1G29280.1 45.055 91 41 1 316 397 44 134 4.78e-18 84.3
Msa0230250 AT4G24240.1 45.783 83 42 1 315 394 256 338 7.25e-18 85.5
Msa0230250 AT3G04670.1 47.059 85 42 1 315 396 237 321 7.41e-18 85.1
Msa0230250 AT3G58710.1 54.098 61 28 0 337 397 71 131 4.62e-17 81.6
Msa0230250 AT3G58710.2 54.098 61 28 0 337 397 70 130 5.00e-17 81.6
Msa0230250 AT5G07100.1 35.294 136 80 5 265 397 163 293 2.06e-16 80.5
Msa0230250 AT5G07100.1 43.056 72 40 1 330 401 110 180 1.54e-11 65.9
Msa0230250 AT4G30935.1 40.000 105 58 3 294 397 290 390 2.31e-16 81.6
Msa0230250 AT2G30590.1 55.932 59 26 0 338 396 314 372 3.61e-16 80.5
Msa0230250 AT5G07100.2 35.294 136 80 5 265 397 70 200 4.51e-16 77.4
Msa0230250 AT5G07100.2 42.466 73 41 1 330 402 17 88 3.89e-11 63.2
Msa0230250 AT5G07100.4 35.294 136 80 5 265 397 70 200 4.51e-16 77.4
Msa0230250 AT5G07100.4 42.466 73 41 1 330 402 17 88 3.89e-11 63.2
Msa0230250 AT5G07100.5 35.294 136 80 5 265 397 70 200 4.51e-16 77.4
Msa0230250 AT5G07100.5 42.466 73 41 1 330 402 17 88 3.89e-11 63.2
Msa0230250 AT5G07100.3 35.294 136 80 5 265 397 70 200 4.51e-16 77.4
Msa0230250 AT5G07100.3 42.466 73 41 1 330 402 17 88 3.89e-11 63.2
Msa0230250 AT3G01080.2 48.485 66 33 1 332 397 339 403 4.74e-15 77.8
Msa0230250 AT3G01080.2 45.714 70 36 2 333 402 201 268 1.15e-13 73.6
Msa0230250 AT2G04880.1 35.652 115 60 2 296 397 253 366 5.11e-15 77.8
Msa0230250 AT2G04880.2 35.115 131 66 4 296 413 229 353 5.13e-15 77.4
Msa0230250 AT3G01080.1 48.485 66 33 1 332 397 301 365 6.07e-15 77.0
Msa0230250 AT3G01080.1 45.714 70 36 2 333 402 163 230 1.51e-13 72.8
Msa0230250 AT4G26640.7 41.667 72 41 1 326 397 330 400 3.36e-14 75.1
Msa0230250 AT4G26640.7 34.146 123 71 3 292 414 134 246 1.56e-11 67.0
Msa0230250 AT4G26640.5 41.667 72 41 1 326 397 194 264 3.85e-14 74.3
Msa0230250 AT4G26640.5 41.250 80 45 2 335 414 33 110 4.19e-11 65.1
Msa0230250 AT4G26640.6 41.667 72 41 1 326 397 194 264 3.85e-14 74.3
Msa0230250 AT4G26640.6 41.250 80 45 2 335 414 33 110 4.19e-11 65.1
Msa0230250 AT4G26640.4 41.667 72 41 1 326 397 194 264 3.85e-14 74.3
Msa0230250 AT4G26640.4 41.250 80 45 2 335 414 33 110 4.19e-11 65.1
Msa0230250 AT4G26640.3 41.667 72 41 1 326 397 194 264 3.85e-14 74.3
Msa0230250 AT4G26640.3 41.250 80 45 2 335 414 33 110 4.19e-11 65.1
Msa0230250 AT4G26640.2 41.667 72 41 1 326 397 370 440 3.98e-14 75.1
Msa0230250 AT4G26640.2 34.146 123 71 3 292 414 174 286 1.74e-11 67.0
Msa0230250 AT4G26640.1 41.667 72 41 1 326 397 298 368 4.54e-14 74.7
Msa0230250 AT4G26640.1 34.146 123 71 3 292 414 102 214 2.40e-11 66.2
Msa0230250 AT3G01080.3 45.714 70 36 2 333 402 201 268 4.66e-14 73.6
Msa0230250 AT2G37260.1 41.463 82 43 2 317 394 325 405 5.37e-14 74.3
Msa0230250 AT2G37260.1 50.769 65 30 2 333 397 161 223 5.67e-12 67.8
Msa0230250 AT1G69810.2 43.590 78 43 1 332 408 140 217 6.18e-14 73.2
Msa0230250 AT1G69810.1 43.590 78 43 1 332 408 198 275 8.15e-14 73.6
Msa0230250 AT2G37260.3 41.463 82 43 2 317 394 245 325 8.74e-14 73.2
Msa0230250 AT2G37260.3 50.769 65 30 2 333 397 81 143 9.54e-12 66.6
Msa0230250 AT2G37260.2 41.463 82 43 2 317 394 243 323 9.43e-14 72.8
Msa0230250 AT2G37260.2 50.769 65 30 2 333 397 79 141 1.04e-11 66.6
Msa0230250 AT1G13960.1 43.939 66 36 1 332 397 404 468 1.02e-13 73.6
Msa0230250 AT1G13960.1 44.928 69 36 2 333 401 225 291 3.89e-12 68.9
Msa0230250 AT2G03340.1 43.939 66 36 1 332 397 410 474 1.11e-13 73.6
Msa0230250 AT2G03340.1 35.484 124 63 4 333 444 246 364 4.28e-12 68.6
Msa0230250 AT2G38470.1 51.471 68 31 2 331 398 178 243 1.22e-13 73.6
Msa0230250 AT2G38470.1 46.667 60 31 1 338 397 363 421 3.72e-13 72.0
Msa0230250 AT1G13960.2 43.939 66 36 1 332 397 251 315 2.12e-13 72.0
Msa0230250 AT1G13960.2 44.928 69 36 2 333 401 72 138 9.96e-12 66.6
Msa0230250 AT5G26170.1 47.619 63 32 1 332 394 108 169 4.29e-13 67.8
Msa0230250 AT4G04450.2 36.145 83 53 0 332 414 193 275 8.36e-13 70.5
Msa0230250 AT4G22070.4 39.394 66 40 0 332 397 292 357 1.42e-12 70.1
Msa0230250 AT4G22070.3 39.394 66 40 0 332 397 292 357 1.42e-12 70.1
Msa0230250 AT4G22070.2 39.394 66 40 0 332 397 292 357 1.42e-12 70.1
Msa0230250 AT4G22070.1 39.394 66 40 0 332 397 292 357 1.42e-12 70.1
Msa0230250 AT2G30250.1 45.312 64 34 1 338 401 329 391 1.52e-12 69.7
Msa0230250 AT4G04450.1 36.145 83 53 0 332 414 287 369 1.79e-12 69.7
Msa0230250 AT1G80840.1 37.805 82 47 1 323 400 128 209 1.82e-12 68.6
Msa0230250 AT4G26440.1 38.542 96 53 3 325 420 170 259 2.00e-12 69.7
Msa0230250 AT2G47260.1 36.667 90 55 2 308 397 146 233 3.40e-12 68.2
Msa0230250 AT1G62300.1 40.984 61 36 0 337 397 312 372 4.71e-12 68.6
Msa0230250 AT5G43290.1 51.667 60 28 1 335 394 112 170 8.25e-12 66.2
Msa0230250 AT1G69310.4 37.805 82 49 2 314 395 125 204 1.23e-11 65.9
Msa0230250 AT1G69310.2 37.805 82 49 2 314 395 125 204 1.23e-11 65.9
Msa0230250 AT1G69310.1 37.805 82 49 2 314 395 125 204 1.23e-11 65.9
Msa0230250 AT1G55600.1 38.356 73 43 2 326 397 295 366 1.49e-11 67.0
Msa0230250 AT5G56270.1 50.000 60 28 2 338 397 274 331 1.55e-11 67.0
Msa0230250 AT5G56270.1 43.333 60 33 1 338 397 488 546 1.82e-11 67.0
Msa0230250 AT4G18170.1 29.365 126 82 3 277 402 118 236 2.33e-11 65.5
Msa0230250 AT4G31800.2 40.000 60 36 0 338 397 112 171 2.92e-11 63.9
Msa0230250 AT4G31800.1 40.000 60 36 0 338 397 177 236 4.11e-11 64.3
Msa0230250 AT4G12020.5 33.333 129 68 6 333 449 397 519 4.58e-11 66.2
Msa0230250 AT1G29860.1 38.272 81 47 2 327 405 124 203 5.04e-11 63.9
Msa0230250 AT5G49520.1 37.179 78 47 2 320 397 205 280 5.45e-11 64.7
Msa0230250 AT4G12020.10 33.333 129 68 6 333 449 464 586 6.17e-11 65.9
Msa0230250 AT4G12020.1 33.333 129 68 6 333 449 464 586 6.19e-11 65.9
Msa0230250 AT4G12020.9 33.333 129 68 6 333 449 464 586 6.19e-11 65.9
Msa0230250 AT4G12020.6 33.333 129 68 6 333 449 464 586 6.22e-11 65.9
Msa0230250 AT4G12020.8 33.333 129 68 6 333 449 464 586 6.22e-11 65.9
Msa0230250 AT4G12020.4 33.333 129 68 6 333 449 464 586 6.23e-11 65.9
Msa0230250 AT4G12020.7 33.333 129 68 6 333 449 464 586 6.24e-11 65.9
Msa0230250 AT4G12020.3 33.333 129 68 6 333 449 464 586 6.68e-11 65.5
Msa0230250 AT4G12020.2 33.333 129 68 6 333 449 464 586 6.69e-11 65.5
Msa0230250 AT1G18860.1 43.333 60 34 0 338 397 240 299 6.74e-11 65.1
Msa0230250 AT3G62340.1 40.000 85 47 3 314 397 96 177 6.93e-11 63.5
Msa0230250 AT5G15130.2 37.662 77 46 1 323 397 169 245 7.60e-11 64.7
Msa0230250 AT5G41570.1 31.183 93 61 2 304 394 63 154 8.39e-11 61.2

Find 112 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTCTGAAACAGAATTTAT+TGG 0.120089 2_2:+2273911 Msa0230250:CDS
TAGAAACCCAAACTAGTTCT+AGG 0.250090 2_2:+2272328 Msa0230250:CDS
ACAAAGTTCTTAATTTCTTC+TGG 0.267372 2_2:-2273797 None:intergenic
GTCGGGGTGGTGGTGGTTGA+AGG 0.269792 2_2:-2271793 None:intergenic
GTAAAACCCTAGAACTAGTT+TGG 0.270284 2_2:-2272335 None:intergenic
GGGGATAATGGTGGTGGTGG+TGG 0.286343 2_2:-2271767 None:intergenic
AAGGTGGTGGTAGTGGTAAT+TGG 0.290628 2_2:-2272018 None:intergenic
CTAGTTTGGGTTTCTAGAAT+TGG 0.312695 2_2:-2272321 None:intergenic
CAAACCTCAGATCAAGAAAA+AGG 0.317679 2_2:+2272379 Msa0230250:CDS
GGTTGAAGGGGTGGGGATAA+TGG 0.324698 2_2:-2271779 None:intergenic
GGTGGTGGTGGTTGAAGGGG+TGG 0.333560 2_2:-2271788 None:intergenic
AGGTGGTGGTAGTGGTAATT+GGG 0.338809 2_2:-2272017 None:intergenic
GGTAATTGGGGTAATGATAG+TGG 0.346701 2_2:-2272004 None:intergenic
AGGGATAATTGTGGTGGGAT+TGG 0.353569 2_2:-2271710 None:intergenic
ATACGGGTGCTAGCGGGGTT+AGG 0.359448 2_2:-2271730 None:intergenic
TGGTGGTGGTTGAAGGGGTG+GGG 0.363221 2_2:-2271786 None:intergenic
CGGGGTGGTGGTGGTTGAAG+GGG 0.364013 2_2:-2271791 None:intergenic
CTTCAATGGATAATGATGAT+TGG 0.367851 2_2:+2271443 None:intergenic
TATTATTAGTGATGTGGGTC+GGG 0.369132 2_2:-2271810 None:intergenic
TTCAATGGATAATGATGATT+GGG 0.373937 2_2:+2271444 None:intergenic
GGGAATGTGAATAGCGGTGG+TGG 0.376751 2_2:-2271959 None:intergenic
GCTTATATTGTGACGTATAA+AGG 0.379319 2_2:+2273755 Msa0230250:CDS
GTGGTGGTGGTTAGGATTTG+AGG 0.379807 2_2:-2271547 None:intergenic
GATTGTTGCTGATTTGTGTT+TGG 0.383802 2_2:-2272357 None:intergenic
GTGGTGGTGGTTGAAGGGGT+GGG 0.384113 2_2:-2271787 None:intergenic
TCTAACTCTATGAAGTCATT+TGG 0.386725 2_2:-2271670 None:intergenic
TACTGATGGTGGTGGTGGTT+AGG 0.393621 2_2:-2271555 None:intergenic
TCTGACATAGCTGTTATGTT+TGG 0.394725 2_2:-2273965 None:intergenic
AGTGATGTGGGTCGGGGTGG+TGG 0.395367 2_2:-2271803 None:intergenic
TAAAACCCTAGAACTAGTTT+GGG 0.406708 2_2:-2272334 None:intergenic
GGTGGGGATAATGGTGGTGG+TGG 0.411568 2_2:-2271770 None:intergenic
AATTGTGGTGGGATTGGAAT+TGG 0.413486 2_2:-2271704 None:intergenic
TCGGGGTGGTGGTGGTTGAA+GGG 0.422426 2_2:-2271792 None:intergenic
TGAAGGGGTGGGGATAATGG+TGG 0.425891 2_2:-2271776 None:intergenic
ACTAACACTAGGATATTGAT+TGG 0.429065 2_2:-2272136 None:intergenic
GAAATTAAGAACTTTGTTGT+TGG 0.434538 2_2:+2273803 Msa0230250:CDS
TATGTCAGAAGATTTATTGA+TGG 0.435423 2_2:+2273979 Msa0230250:CDS
GATGTGGGTCGGGGTGGTGG+TGG 0.445392 2_2:-2271800 None:intergenic
TACGGGTGCTAGCGGGGTTA+GGG 0.449094 2_2:-2271729 None:intergenic
TGGAGTTAGAGTTTCAGAAT+TGG 0.449537 2_2:-2271524 None:intergenic
TGTGTATGTTGTGGACTAGA+TGG 0.450832 2_2:-2272282 None:intergenic
AAAGGTGGAATTTCGATTTG+AGG 0.455769 2_2:-2271851 None:intergenic
GACTTTAACCACCTCAATAG+TGG 0.457259 2_2:+2274001 Msa0230250:CDS
GGAATGTGAATAGCGGTGGT+GGG 0.459869 2_2:-2271958 None:intergenic
AGGGGTGGGGATAATGGTGG+TGG 0.468517 2_2:-2271773 None:intergenic
ACCTGAGAATTGCTAGTCTC+TGG 0.471222 2_2:-2273863 None:intergenic
GAGCATAACATCTTCTACTT+CGG 0.472162 2_2:-2271926 None:intergenic
TGAAGTCATTTGGTTTATGC+GGG 0.474842 2_2:-2271660 None:intergenic
GTTAATATTATTAGTGATGT+GGG 0.476825 2_2:-2271815 None:intergenic
GCCAGAGACTAGCAATTCTC+AGG 0.477878 2_2:+2273862 Msa0230250:CDS
AGCGGTTGTGATTTCAATGC+AGG 0.486927 2_2:-2272235 None:intergenic
ATATTATTAGTGATGTGGGT+CGG 0.491960 2_2:-2271811 None:intergenic
TTTGGAATACGGGTGCTAGC+GGG 0.501625 2_2:-2271736 None:intergenic
ATAACACACCAAGGACATCT+TGG 0.504754 2_2:-2272544 None:intergenic
TTGCTTCTTTGTGTATGTTG+TGG 0.506190 2_2:-2272291 None:intergenic
AGAAACCCAAACTAGTTCTA+GGG 0.507577 2_2:+2272329 Msa0230250:CDS
TGTCCTTGGTGTGTTATATG+AGG 0.509380 2_2:+2272550 Msa0230250:CDS
GTTAGCGAAGGTGGTGGTAG+TGG 0.510257 2_2:-2272025 None:intergenic
CAAGGTGCTAATAGCGTTGG+CGG 0.513265 2_2:-2272049 None:intergenic
GTTGGCGGGGTTAGCGAAGG+TGG 0.515885 2_2:-2272034 None:intergenic
ATGAAGTCATTTGGTTTATG+CGG 0.518878 2_2:-2271661 None:intergenic
ATTATTAGTGATGTGGGTCG+GGG 0.521424 2_2:-2271809 None:intergenic
GAATGTGAATAGCGGTGGTG+GGG 0.524266 2_2:-2271957 None:intergenic
GGTAGGAGTAGTGGCAGTCA+AGG 0.527115 2_2:-2272067 None:intergenic
AGTTAGAGTTTCAGAATTGG+TGG 0.535695 2_2:-2271521 None:intergenic
GGAATAAAGCACCACTATTG+AGG 0.537769 2_2:-2274012 None:intergenic
GGCGGGGTTAGCGAAGGTGG+TGG 0.538342 2_2:-2272031 None:intergenic
TGTTAATATTATTAGTGATG+TGG 0.542659 2_2:-2271816 None:intergenic
AAGGTGCTAATAGCGTTGGC+GGG 0.545221 2_2:-2272048 None:intergenic
GGTGGTGAGAGTAGTGATGG+TGG 0.545701 2_2:-2271980 None:intergenic
GATAGTGTTGGCAGTAGAGA+TGG 0.547801 2_2:-2272088 None:intergenic
GTAATTGGGGTAATGATAGT+GGG 0.548235 2_2:-2272003 None:intergenic
GATGTTACTGATGGTGGTGG+TGG 0.548240 2_2:-2271560 None:intergenic
GTGGTGGTTAGGATTTGAGG+TGG 0.552967 2_2:-2271544 None:intergenic
AAAGAATGCCAAGATGTCCT+TGG 0.553216 2_2:+2272536 Msa0230250:CDS
AGTAGAGATGGTAGGAGTAG+TGG 0.558152 2_2:-2272076 None:intergenic
GGGTTAGGGATAATTGTGGT+GGG 0.569034 2_2:-2271715 None:intergenic
GGATATGATGTTACTGATGG+TGG 0.569473 2_2:-2271566 None:intergenic
TATGTTGTGGACTAGATGGA+TGG 0.570891 2_2:-2272278 None:intergenic
TGAACTTTCAACAGACTCAT+GGG 0.572196 2_2:+2272578 Msa0230250:CDS
GGTGGCAGAAGTAGTAAAGG+TGG 0.572647 2_2:-2271503 None:intergenic
TTAAAGGGTCTCCACATCCA+AGG 0.575832 2_2:+2272628 Msa0230250:CDS
ATTGGTGGCAGAAGTAGTAA+AGG 0.577716 2_2:-2271506 None:intergenic
GTGGTGAGAGTAGTGATGGT+GGG 0.578827 2_2:-2271979 None:intergenic
ATGTATGAAAACCTTGGATG+TGG 0.583670 2_2:-2272639 None:intergenic
ATGAACTTTCAACAGACTCA+TGG 0.586902 2_2:+2272577 Msa0230250:CDS
TGTTGTTGTTCAATGAGTGT+TGG 0.587742 2_2:-2271883 None:intergenic
GTGTTGGCAGTAGAGATGGT+AGG 0.592781 2_2:-2272084 None:intergenic
ATTAGTGATGTGGGTCGGGG+TGG 0.595677 2_2:-2271806 None:intergenic
TTGGAATACGGGTGCTAGCG+GGG 0.599630 2_2:-2271735 None:intergenic
AGTCAAGGTGCTAATAGCGT+TGG 0.600403 2_2:-2272052 None:intergenic
GGGGTTAGGGATAATTGTGG+TGG 0.606726 2_2:-2271716 None:intergenic
GGTGGGAATGTGAATAGCGG+TGG 0.610840 2_2:-2271962 None:intergenic
TCATGGGCATGGAGTAAACA+TGG 0.611520 2_2:+2272594 Msa0230250:CDS
AGTGTTCGTCATGATAGTGT+TGG 0.615034 2_2:-2272100 None:intergenic
AATTGGGGTAATGATAGTGG+GGG 0.618091 2_2:-2272001 None:intergenic
TAATTGGGGTAATGATAGTG+GGG 0.618793 2_2:-2272002 None:intergenic
TTTCAACAGACTCATGGGCA+TGG 0.620196 2_2:+2272583 Msa0230250:CDS
ATCATCATAGCATGATGATG+TGG 0.621279 2_2:-2271599 None:intergenic
AGCGTTGGCGGGGTTAGCGA+AGG 0.628213 2_2:-2272037 None:intergenic
TGATGATGTGGTACTTTGAG+AGG 0.630706 2_2:-2271587 None:intergenic
GGTGGTGGTAGTGGTAATTG+GGG 0.635605 2_2:-2272016 None:intergenic
TATGATGTTACTGATGGTGG+TGG 0.635627 2_2:-2271563 None:intergenic
AGAGGATATGATGTTACTGA+TGG 0.644683 2_2:-2271569 None:intergenic
GAAGTCATTTGGTTTATGCG+GGG 0.651223 2_2:-2271659 None:intergenic
TTCCCTCATATAACACACCA+AGG 0.662868 2_2:-2272553 None:intergenic
ATAAAGCACCACTATTGAGG+TGG 0.665488 2_2:-2274009 None:intergenic
AGCGGGGTTAGGGATAATTG+TGG 0.672234 2_2:-2271719 None:intergenic
GTCCTTGGTGTGTTATATGA+GGG 0.673519 2_2:+2272551 Msa0230250:CDS
AGGTGCTAATAGCGTTGGCG+GGG 0.696052 2_2:-2272047 None:intergenic
GATGGTGGGAATGTGAATAG+CGG 0.710352 2_2:-2271965 None:intergenic
GTATGCACGATCAAAATGAG+CGG 0.723045 2_2:-2272253 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAATAAAAAATATATAT+AGG + chr2_2:2273082-2273101 Msa0230250:intron 0.0%
!!! TTTATTTTATTTTTTGTCTT+TGG - chr2_2:2273072-2273091 None:intergenic 10.0%
!!! ATAGAAAAAGTAATTTTACA+GGG - chr2_2:2272831-2272850 None:intergenic 15.0%
!!! AATAGAAAAAGTAATTTTAC+AGG - chr2_2:2272832-2272851 None:intergenic 15.0%
!!! CAATTTCAATATTTTTTCTT+AGG - chr2_2:2272906-2272925 None:intergenic 15.0%
!!! ATTTTAATCCTTATTTTGAT+GGG + chr2_2:2272941-2272960 Msa0230250:intron 15.0%
!! ATTAAATATTCTACATATAG+AGG + chr2_2:2272969-2272988 Msa0230250:intron 15.0%
!! TTAATATGAATAATATTTGC+AGG + chr2_2:2273000-2273019 Msa0230250:intron 15.0%
!! TAATATGAATAATATTTGCA+GGG + chr2_2:2273001-2273020 Msa0230250:intron 15.0%
!!! TGGTAATAATTTTTGGAATA+CGG - chr2_2:2271750-2271769 None:intergenic 20.0%
!! GTTAATATTATTAGTGATGT+GGG - chr2_2:2271818-2271837 None:intergenic 20.0%
!! TGTTAATATTATTAGTGATG+TGG - chr2_2:2271819-2271838 None:intergenic 20.0%
!!! TTTTTTCTGTAAGAAAAGAA+AGG - chr2_2:2272519-2272538 None:intergenic 20.0%
!! ATGGACAAAAATTCATTAAA+GGG + chr2_2:2272613-2272632 Msa0230250:CDS 20.0%
!!! CATTTTAATCCTTATTTTGA+TGG + chr2_2:2272940-2272959 Msa0230250:intron 20.0%
!! TAAAGTGAAGATATAAACTT+AGG - chr2_2:2273209-2273228 None:intergenic 20.0%
!! TGATAAACAAACAATTGATA+CGG + chr2_2:2273242-2273261 Msa0230250:intron 20.0%
!!! TTTATATAAAGGTATGCAAT+CGG - chr2_2:2273357-2273376 None:intergenic 20.0%
!! AAGAGATTGCATTATTTTCT+TGG - chr2_2:2271636-2271655 None:intergenic 25.0%
!!! GGTAATAATTTTTGGAATAC+GGG - chr2_2:2271749-2271768 None:intergenic 25.0%
! GTAAGAAAAGAAAGGTAAAA+TGG - chr2_2:2272511-2272530 None:intergenic 25.0%
! CATGGACAAAAATTCATTAA+AGG + chr2_2:2272612-2272631 Msa0230250:CDS 25.0%
!! TTTTGAATGTATGAAAACCT+TGG - chr2_2:2272648-2272667 None:intergenic 25.0%
!! AAATAGTGACAGTAAACTTT+TGG + chr2_2:2272753-2272772 Msa0230250:intron 25.0%
!!! AATTTTAGCCCATCAAAATA+AGG - chr2_2:2272952-2272971 None:intergenic 25.0%
! TATCAGTGCTCTTTATATAA+AGG - chr2_2:2273368-2273387 None:intergenic 25.0%
! TTAATCATTCTTTGCAATCT+TGG - chr2_2:2273466-2273485 None:intergenic 25.0%
!! TGATTTGTATCTTTAACAGT+AGG + chr2_2:2273493-2273512 Msa0230250:intron 25.0%
! TCTTTAACAGTAGGAAAATA+CGG + chr2_2:2273502-2273521 Msa0230250:intron 25.0%
!! GAAATCTATGAATGATTTGT+AGG + chr2_2:2273657-2273676 Msa0230250:intron 25.0%
!!! TTCTCTACATATTTTTTTGC+TGG - chr2_2:2273716-2273735 None:intergenic 25.0%
!!! TCTTAATTTCTTCTGGTTTT+TGG - chr2_2:2273793-2273812 None:intergenic 25.0%
! ACAAAGTTCTTAATTTCTTC+TGG - chr2_2:2273800-2273819 None:intergenic 25.0%
!! GAAATTAAGAACTTTGTTGT+TGG + chr2_2:2273803-2273822 Msa0230250:CDS 25.0%
!!! ACAAGAGATTTTTGATTCAT+CGG - chr2_2:2273894-2273913 None:intergenic 25.0%
!!! TGTTCTGAAACAGAATTTAT+TGG + chr2_2:2273911-2273930 Msa0230250:CDS 25.0%
! TATGTCAGAAGATTTATTGA+TGG + chr2_2:2273979-2273998 Msa0230250:CDS 25.0%
! ATGAAGTCATTTGGTTTATG+CGG - chr2_2:2271664-2271683 None:intergenic 30.0%
TCTAACTCTATGAAGTCATT+TGG - chr2_2:2271673-2271692 None:intergenic 30.0%
ATATTATTAGTGATGTGGGT+CGG - chr2_2:2271814-2271833 None:intergenic 30.0%
ACTAACACTAGGATATTGAT+TGG - chr2_2:2272139-2272158 None:intergenic 30.0%
GTTGAAAAATCACTAACACT+AGG - chr2_2:2272150-2272169 None:intergenic 30.0%
! TAAAACCCTAGAACTAGTTT+GGG - chr2_2:2272337-2272356 None:intergenic 30.0%
! ATTACCTTTTTCTTGATCTG+AGG - chr2_2:2272386-2272405 None:intergenic 30.0%
! GTTCGATGAGATTTAAGTTA+TGG - chr2_2:2272483-2272502 None:intergenic 30.0%
! CTATTTAAAACATGTGGTGT+GGG - chr2_2:2272739-2272758 None:intergenic 30.0%
! ACTATTTAAAACATGTGGTG+TGG - chr2_2:2272740-2272759 None:intergenic 30.0%
! CTGTCACTATTTAAAACATG+TGG - chr2_2:2272745-2272764 None:intergenic 30.0%
ATCCTTGCAATTTCAAAGAA+TGG + chr2_2:2272776-2272795 Msa0230250:intron 30.0%
!! TACCATTCTTTGAAATTGCA+AGG - chr2_2:2272781-2272800 None:intergenic 30.0%
TAAAAACCAAAAGCCAAAGA+TGG + chr2_2:2273039-2273058 Msa0230250:intron 30.0%
!!! AATAAACCATCTTTGGCTTT+TGG - chr2_2:2273048-2273067 None:intergenic 30.0%
! CTTTGGAAATAAACCATCTT+TGG - chr2_2:2273055-2273074 None:intergenic 30.0%
ACACATATATATGCCATGAT+GGG + chr2_2:2273112-2273131 Msa0230250:intron 30.0%
!! ATATGTTTTTGATCCCATCA+TGG - chr2_2:2273128-2273147 None:intergenic 30.0%
TTGCTCAATGGAAAAAGATA+CGG - chr2_2:2273425-2273444 None:intergenic 30.0%
! CTTTTTCCATTGAGCAATTA+AGG + chr2_2:2273428-2273447 Msa0230250:intron 30.0%
ATGATATTCTAACAACCTAC+AGG - chr2_2:2273537-2273556 None:intergenic 30.0%
GCTTATATTGTGACGTATAA+AGG + chr2_2:2273755-2273774 Msa0230250:CDS 30.0%
AGTTAGAGTTTCAGAATTGG+TGG - chr2_2:2271524-2271543 None:intergenic 35.0%
TGGAGTTAGAGTTTCAGAAT+TGG - chr2_2:2271527-2271546 None:intergenic 35.0%
AGAGGATATGATGTTACTGA+TGG - chr2_2:2271572-2271591 None:intergenic 35.0%
! ATCATCATAGCATGATGATG+TGG - chr2_2:2271602-2271621 None:intergenic 35.0%
! TGAAGTCATTTGGTTTATGC+GGG - chr2_2:2271663-2271682 None:intergenic 35.0%
!!! GTGGTGGTGGTAATAATTTT+TGG - chr2_2:2271757-2271776 None:intergenic 35.0%
TATTATTAGTGATGTGGGTC+GGG - chr2_2:2271813-2271832 None:intergenic 35.0%
! AAAGGTGGAATTTCGATTTG+AGG - chr2_2:2271854-2271873 None:intergenic 35.0%
TGTTGGAAGATGAAAAAAGG+TGG - chr2_2:2271869-2271888 None:intergenic 35.0%
!! GAGTGTTGGAAGATGAAAAA+AGG - chr2_2:2271872-2271891 None:intergenic 35.0%
! TGTTGTTGTTCAATGAGTGT+TGG - chr2_2:2271886-2271905 None:intergenic 35.0%
GAGCATAACATCTTCTACTT+CGG - chr2_2:2271929-2271948 None:intergenic 35.0%
! TAATTGGGGTAATGATAGTG+GGG - chr2_2:2272005-2272024 None:intergenic 35.0%
GTAATTGGGGTAATGATAGT+GGG - chr2_2:2272006-2272025 None:intergenic 35.0%
TTGCTTCTTTGTGTATGTTG+TGG - chr2_2:2272294-2272313 None:intergenic 35.0%
!! CTAGTTTGGGTTTCTAGAAT+TGG - chr2_2:2272324-2272343 None:intergenic 35.0%
! TAGAAACCCAAACTAGTTCT+AGG + chr2_2:2272328-2272347 Msa0230250:CDS 35.0%
!! AGAAACCCAAACTAGTTCTA+GGG + chr2_2:2272329-2272348 Msa0230250:CDS 35.0%
GTAAAACCCTAGAACTAGTT+TGG - chr2_2:2272338-2272357 None:intergenic 35.0%
!! GATTGTTGCTGATTTGTGTT+TGG - chr2_2:2272360-2272379 None:intergenic 35.0%
CAAACCTCAGATCAAGAAAA+AGG + chr2_2:2272379-2272398 Msa0230250:CDS 35.0%
ATGAACTTTCAACAGACTCA+TGG + chr2_2:2272577-2272596 Msa0230250:CDS 35.0%
TGAACTTTCAACAGACTCAT+GGG + chr2_2:2272578-2272597 Msa0230250:CDS 35.0%
ATGTATGAAAACCTTGGATG+TGG - chr2_2:2272642-2272661 None:intergenic 35.0%
CATTGCAAATATTCCACCTT+TGG + chr2_2:2272852-2272871 Msa0230250:intron 35.0%
AGGAACTATAACAAACACCA+GGG - chr2_2:2272886-2272905 None:intergenic 35.0%
TAGGAACTATAACAAACACC+AGG - chr2_2:2272887-2272906 None:intergenic 35.0%
CACACATATATATGCCATGA+TGG + chr2_2:2273111-2273130 Msa0230250:intron 35.0%
AAGAAATATGCCACTCATTG+TGG + chr2_2:2273324-2273343 Msa0230250:intron 35.0%
AGAAATATGCCACTCATTGT+GGG + chr2_2:2273325-2273344 Msa0230250:intron 35.0%
!!! TGTGGAAAGTTGTTGGTTTT+TGG - chr2_2:2273400-2273419 None:intergenic 35.0%
TTCTTTGCAATCTTGGAAAG+CGG - chr2_2:2273459-2273478 None:intergenic 35.0%
!! CAGCATCATTTTGTTATGTG+AGG - chr2_2:2273831-2273850 None:intergenic 35.0%
TCTGACATAGCTGTTATGTT+TGG - chr2_2:2273968-2273987 None:intergenic 35.0%
! ATTGGTGGCAGAAGTAGTAA+AGG - chr2_2:2271509-2271528 None:intergenic 40.0%
!! TATGATGTTACTGATGGTGG+TGG - chr2_2:2271566-2271585 None:intergenic 40.0%
GGATATGATGTTACTGATGG+TGG - chr2_2:2271569-2271588 None:intergenic 40.0%
! TGATGATGTGGTACTTTGAG+AGG - chr2_2:2271590-2271609 None:intergenic 40.0%
! GAAGTCATTTGGTTTATGCG+GGG - chr2_2:2271662-2271681 None:intergenic 40.0%
! AATTGTGGTGGGATTGGAAT+TGG - chr2_2:2271707-2271726 None:intergenic 40.0%
ATTATTAGTGATGTGGGTCG+GGG - chr2_2:2271812-2271831 None:intergenic 40.0%
!! AATTGGGGTAATGATAGTGG+GGG - chr2_2:2272004-2272023 None:intergenic 40.0%
GGTAATTGGGGTAATGATAG+TGG - chr2_2:2272007-2272026 None:intergenic 40.0%
! AGTGTTCGTCATGATAGTGT+TGG - chr2_2:2272103-2272122 None:intergenic 40.0%
GTATGCACGATCAAAATGAG+CGG - chr2_2:2272256-2272275 None:intergenic 40.0%
!! TATGTTGTGGACTAGATGGA+TGG - chr2_2:2272281-2272300 None:intergenic 40.0%
!! TGTGTATGTTGTGGACTAGA+TGG - chr2_2:2272285-2272304 None:intergenic 40.0%
AAAGAATGCCAAGATGTCCT+TGG + chr2_2:2272536-2272555 Msa0230250:CDS 40.0%
ATAACACACCAAGGACATCT+TGG - chr2_2:2272547-2272566 None:intergenic 40.0%
! TGTCCTTGGTGTGTTATATG+AGG + chr2_2:2272550-2272569 Msa0230250:CDS 40.0%
! GTCCTTGGTGTGTTATATGA+GGG + chr2_2:2272551-2272570 Msa0230250:CDS 40.0%
TTCCCTCATATAACACACCA+AGG - chr2_2:2272556-2272575 None:intergenic 40.0%
ATGGAAAAAGATACGGTGTG+TGG - chr2_2:2273418-2273437 None:intergenic 40.0%
TTCCATTGAGCAATTAAGGC+TGG + chr2_2:2273432-2273451 Msa0230250:intron 40.0%
TCTTTGCAATCTTGGAAAGC+GGG - chr2_2:2273458-2273477 None:intergenic 40.0%
AACAGTAGGAAAATACGGTG+TGG + chr2_2:2273507-2273526 Msa0230250:intron 40.0%
GACTTTAACCACCTCAATAG+TGG + chr2_2:2274001-2274020 Msa0230250:CDS 40.0%
! ATAAAGCACCACTATTGAGG+TGG - chr2_2:2274012-2274031 None:intergenic 40.0%
! GGAATAAAGCACCACTATTG+AGG - chr2_2:2274015-2274034 None:intergenic 40.0%
! AGGGATAATTGTGGTGGGAT+TGG - chr2_2:2271713-2271732 None:intergenic 45.0%
GGGTTAGGGATAATTGTGGT+GGG - chr2_2:2271718-2271737 None:intergenic 45.0%
!!! TTTTGGAATACGGGTGCTAG+CGG - chr2_2:2271740-2271759 None:intergenic 45.0%
!! GATGGTGGGAATGTGAATAG+CGG - chr2_2:2271968-2271987 None:intergenic 45.0%
!! AGGTGGTGGTAGTGGTAATT+GGG - chr2_2:2272020-2272039 None:intergenic 45.0%
!! AAGGTGGTGGTAGTGGTAAT+TGG - chr2_2:2272021-2272040 None:intergenic 45.0%
! AGTCAAGGTGCTAATAGCGT+TGG - chr2_2:2272055-2272074 None:intergenic 45.0%
AGTAGAGATGGTAGGAGTAG+TGG - chr2_2:2272079-2272098 None:intergenic 45.0%
!! GATAGTGTTGGCAGTAGAGA+TGG - chr2_2:2272091-2272110 None:intergenic 45.0%
AGCGGTTGTGATTTCAATGC+AGG - chr2_2:2272238-2272257 None:intergenic 45.0%
TTTCAACAGACTCATGGGCA+TGG + chr2_2:2272583-2272602 Msa0230250:CDS 45.0%
TCATGGGCATGGAGTAAACA+TGG + chr2_2:2272594-2272613 Msa0230250:CDS 45.0%
TTAAAGGGTCTCCACATCCA+AGG + chr2_2:2272628-2272647 Msa0230250:CDS 45.0%
CAGGGACAAAAAACCAAAGG+TGG - chr2_2:2272868-2272887 None:intergenic 45.0%
!!! CACCTTTGGTTTTTTGTCCC+TGG + chr2_2:2272866-2272885 Msa0230250:intron 45.0%
CACCAGGGACAAAAAACCAA+AGG - chr2_2:2272871-2272890 None:intergenic 45.0%
ATATGCCACTCATTGTGGGA+CGG + chr2_2:2273329-2273348 Msa0230250:intron 45.0%
TACGGTGTGTGGAAAGTTGT+TGG - chr2_2:2273407-2273426 None:intergenic 45.0%
! ACCTGAGAATTGCTAGTCTC+TGG - chr2_2:2273866-2273885 None:intergenic 45.0%
!! AATATTATTCATATTAAAAT+TGG - chr2_2:2272997-2273016 None:intergenic 5.0%
GGTGGCAGAAGTAGTAAAGG+TGG - chr2_2:2271506-2271525 None:intergenic 50.0%
GTGGTGGTTAGGATTTGAGG+TGG - chr2_2:2271547-2271566 None:intergenic 50.0%
!! GTGGTGGTGGTTAGGATTTG+AGG - chr2_2:2271550-2271569 None:intergenic 50.0%
!! TACTGATGGTGGTGGTGGTT+AGG - chr2_2:2271558-2271577 None:intergenic 50.0%
!! GATGTTACTGATGGTGGTGG+TGG - chr2_2:2271563-2271582 None:intergenic 50.0%
GGGGTTAGGGATAATTGTGG+TGG - chr2_2:2271719-2271738 None:intergenic 50.0%
AGCGGGGTTAGGGATAATTG+TGG - chr2_2:2271722-2271741 None:intergenic 50.0%
! TTTGGAATACGGGTGCTAGC+GGG - chr2_2:2271739-2271758 None:intergenic 50.0%
GAATGTGAATAGCGGTGGTG+GGG - chr2_2:2271960-2271979 None:intergenic 50.0%
GGAATGTGAATAGCGGTGGT+GGG - chr2_2:2271961-2271980 None:intergenic 50.0%
GTGGTGAGAGTAGTGATGGT+GGG - chr2_2:2271982-2272001 None:intergenic 50.0%
!! GGTGGTGGTAGTGGTAATTG+GGG - chr2_2:2272019-2272038 None:intergenic 50.0%
!! AAGGTGCTAATAGCGTTGGC+GGG - chr2_2:2272051-2272070 None:intergenic 50.0%
! CAAGGTGCTAATAGCGTTGG+CGG - chr2_2:2272052-2272071 None:intergenic 50.0%
! GTGTTGGCAGTAGAGATGGT+AGG - chr2_2:2272087-2272106 None:intergenic 50.0%
GTGGTGTGGGTGTGTGTATT+AGG - chr2_2:2272726-2272745 None:intergenic 50.0%
GGCCAGCCTTAATTGCTCAA+TGG - chr2_2:2273437-2273456 None:intergenic 50.0%
ATACGGTGTGGACAACCTGT+AGG + chr2_2:2273519-2273538 Msa0230250:intron 50.0%
GCCAGAGACTAGCAATTCTC+AGG + chr2_2:2273862-2273881 Msa0230250:CDS 50.0%
TTGGAATACGGGTGCTAGCG+GGG - chr2_2:2271738-2271757 None:intergenic 55.0%
! TGAAGGGGTGGGGATAATGG+TGG - chr2_2:2271779-2271798 None:intergenic 55.0%
GGTTGAAGGGGTGGGGATAA+TGG - chr2_2:2271782-2271801 None:intergenic 55.0%
ATTAGTGATGTGGGTCGGGG+TGG - chr2_2:2271809-2271828 None:intergenic 55.0%
GGGAATGTGAATAGCGGTGG+TGG - chr2_2:2271962-2271981 None:intergenic 55.0%
GGTGGGAATGTGAATAGCGG+TGG - chr2_2:2271965-2271984 None:intergenic 55.0%
GGTGGTGAGAGTAGTGATGG+TGG - chr2_2:2271983-2272002 None:intergenic 55.0%
!! TGGGGTAATGATAGTGGGGG+TGG - chr2_2:2272001-2272020 None:intergenic 55.0%
! GTTAGCGAAGGTGGTGGTAG+TGG - chr2_2:2272028-2272047 None:intergenic 55.0%
!! AGGTGCTAATAGCGTTGGCG+GGG - chr2_2:2272050-2272069 None:intergenic 55.0%
GGTAGGAGTAGTGGCAGTCA+AGG - chr2_2:2272070-2272089 None:intergenic 55.0%
GTAATGTGTGCGCGCGCAAA+TGG + chr2_2:2273590-2273609 Msa0230250:intron 55.0%
TACGGGTGCTAGCGGGGTTA+GGG - chr2_2:2271732-2271751 None:intergenic 60.0%
ATACGGGTGCTAGCGGGGTT+AGG - chr2_2:2271733-2271752 None:intergenic 60.0%
!! GGGGATAATGGTGGTGGTGG+TGG - chr2_2:2271770-2271789 None:intergenic 60.0%
!! GGTGGGGATAATGGTGGTGG+TGG - chr2_2:2271773-2271792 None:intergenic 60.0%
! AGGGGTGGGGATAATGGTGG+TGG - chr2_2:2271776-2271795 None:intergenic 60.0%
!! TGGTGGTGGTTGAAGGGGTG+GGG - chr2_2:2271789-2271808 None:intergenic 60.0%
!! GTGGTGGTGGTTGAAGGGGT+GGG - chr2_2:2271790-2271809 None:intergenic 60.0%
!! TCGGGGTGGTGGTGGTTGAA+GGG - chr2_2:2271795-2271814 None:intergenic 60.0%
GGGGGTGGTGAGAGTAGTGA+TGG - chr2_2:2271986-2272005 None:intergenic 60.0%
CGGTTCCGTCCCACAATGAG+TGG - chr2_2:2273337-2273356 None:intergenic 60.0%
!! GGTGGTGGTGGTTGAAGGGG+TGG - chr2_2:2271791-2271810 None:intergenic 65.0%
!! CGGGGTGGTGGTGGTTGAAG+GGG - chr2_2:2271794-2271813 None:intergenic 65.0%
!! GTCGGGGTGGTGGTGGTTGA+AGG - chr2_2:2271796-2271815 None:intergenic 65.0%
AGTGATGTGGGTCGGGGTGG+TGG - chr2_2:2271806-2271825 None:intergenic 65.0%
! GTTGGCGGGGTTAGCGAAGG+TGG - chr2_2:2272037-2272056 None:intergenic 65.0%
!! AGCGTTGGCGGGGTTAGCGA+AGG - chr2_2:2272040-2272059 None:intergenic 65.0%
! GATGTGGGTCGGGGTGGTGG+TGG - chr2_2:2271803-2271822 None:intergenic 70.0%
! GGCGGGGTTAGCGAAGGTGG+TGG - chr2_2:2272034-2272053 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 2271448 2274039 2271448 ID=Msa0230250;Name=Msa0230250
chr2_2 mRNA 2271448 2274039 2271448 ID=Msa0230250-mRNA-1;Parent=Msa0230250;Name=Msa0230250-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|0|0|1|1|1|3|0|476
chr2_2 exon 2271448 2272400 2271448 ID=Msa0230250-mRNA-1:exon:805;Parent=Msa0230250-mRNA-1
chr2_2 exon 2272533 2272649 2272533 ID=Msa0230250-mRNA-1:exon:806;Parent=Msa0230250-mRNA-1
chr2_2 exon 2273679 2274039 2273679 ID=Msa0230250-mRNA-1:exon:807;Parent=Msa0230250-mRNA-1
chr2_2 CDS 2271448 2272400 2271448 ID=Msa0230250-mRNA-1:cds;Parent=Msa0230250-mRNA-1
chr2_2 CDS 2272533 2272649 2272533 ID=Msa0230250-mRNA-1:cds;Parent=Msa0230250-mRNA-1
chr2_2 CDS 2273679 2274039 2273679 ID=Msa0230250-mRNA-1:cds;Parent=Msa0230250-mRNA-1
Gene Sequence

>Msa0230250

ATGGATAATGATGATTGGGATCTCTTTTCTATTGTGCGTAGCTGCAACACTACTACCACCTTTACTACTTCTGCCACCAATTCTGAAACTCTAACTCCACCTCAAATCCTAACCACCACCACCATCAGTAACATCATATCCTCTCAAAGTACCACATCATCATGCTATGATGATTTTACTTTTATCCAAGAAAATAATGCAATCTCTTTTGCCCCGCATAAACCAAATGACTTCATAGAGTTAGATAAATTGATGACCAATTCCAATCCCACCACAATTATCCCTAACCCCGCTAGCACCCGTATTCCAAAAATTATTACCACCACCACCACCATTATCCCCACCCCTTCAACCACCACCACCCCGACCCACATCACTAATAATATTAACACTAGTGTTCATGCCTCAAATCGAAATTCCACCTTTTTTCATCTTCCAACACTCATTGAACAACAACAAATGCAACGAAATGAGTTCACCGAAGTAGAAGATGTTATGCTCAAATTCAACCCCACCACCGCTATTCACATTCCCACCATCACTACTCTCACCACCCCCACTATCATTACCCCAATTACCACTACCACCACCTTCGCTAACCCCGCCAACGCTATTAGCACCTTGACTGCCACTACTCCTACCATCTCTACTGCCAACACTATCATGACGAACACTAGCGTTCATGAAACCAATCAATATCCTAGTGTTAGTGATTTTTCAACACTCGTTGAACAACAACATATTCATCAAAGCAACAACAATCAAGTGTTTGTTTCGAAACATGTTGCCTGCATTGAAATCACAACCGCTCATTTTGATCGTGCATACAACCATCCATCTAGTCCACAACATACACAAAGAAGCAATCAACTTCCAATTCTAGAAACCCAAACTAGTTCTAGGGTTTTACCAAACACAAATCAGCAACAATCAAACCTCAGATCAAGAAAAAGAAAAAAGAATGCCAAGATGTCCTTGGTGTGTTATATGAGGGAAGATGAACTTTCAACAGACTCATGGGCATGGAGTAAACATGGACAAAAATTCATTAAAGGGTCTCCACATCCAAGGTGCTATTATAAGTGCAGCAGTTTCGCAAATTGTCCAGCAAAAAAATATGTAGAGAAAAGCAAAACTCATGAGAACGCTTATATTGTGACGTATAAAGGAGAGCATGATCACCAAAAACCAGAAGAAATTAAGAACTTTGTTGTTGGTACCTCACATAACAAAATGATGCTGAAGTTTGATGAGCCAGAGACTAGCAATTCTCAGGTTTTTTCCGATGAATCAAAAATCTCTTGTTCTGAAACAGAATTTATTGGAAACTCTAATGATGATGATGACATTTTGATACCAAACATAACAGCTATGTCAGAAGATTTATTGATGGACTTTAACCACCTCAATAGTGGTGCTTTATTCCCATAG
Protein sequence

>Msa0230250

MDNDDWDLFSIVRSCNTTTTFTTSATNSETLTPPQILTTTTISNIISSQSTTSSCYDDFTFIQENNAISFAPHKPNDFIELDKLMTNSNPTTIIPNPASTRIPKIITTTTTIIPTPSTTTTPTHITNNINTSVHASNRNSTFFHLPTLIEQQQMQRNEFTEVEDVMLKFNPTTAIHIPTITTLTTPTIITPITTTTTFANPANAISTLTATTPTISTANTIMTNTSVHETNQYPSVSDFSTLVEQQHIHQSNNNQVFVSKHVACIEITTAHFDRAYNHPSSPQHTQRSNQLPILETQTSSRVLPNTNQQQSNLRSRKRKKNAKMSLVCYMREDELSTDSWAWSKHGQKFIKGSPHPRCYYKCSSFANCPAKKYVEKSKTHENAYIVTYKGEHDHQKPEEIKNFVVGTSHNKMMLKFDEPETSNSQVFSDESKISCSETEFIGNSNDDDDILIPNITAMSEDLLMDFNHLNSGALFP*