AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0230260

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0230260 MtrunA17_Chr2g0289181 43.777 233 51 3 159 311 1 233 7.27e-57 183
Msa0230260 MtrunA17_Chr6g0470151 41.860 172 82 3 27 195 197 353 2.10e-29 115
Msa0230260 MtrunA17_Chr1g0202461 47.706 109 54 3 138 246 229 334 4.31e-26 106
Msa0230260 MtrunA17_Chr8g0348311 47.000 100 50 2 149 248 134 230 3.53e-23 97.8
Msa0230260 MtrunA17_Chr7g0248691 46.602 103 52 2 146 248 130 229 1.34e-22 95.9
Msa0230260 MtrunA17_Chr6g0470161 40.367 109 42 1 203 311 35 120 2.91e-21 87.4
Msa0230260 MtrunA17_Chr7g0266621 49.558 113 47 2 40 151 199 302 9.44e-21 90.5
Msa0230260 MtrunA17_Chr2g0293481 39.669 121 61 3 137 246 221 340 1.64e-20 91.7
Msa0230260 MtrunA17_Chr8g0387501 40.351 114 66 2 173 286 130 241 5.04e-20 87.8
Msa0230260 MtrunA17_Chr4g0071521 45.783 83 42 1 150 229 220 302 6.01e-20 88.6
Msa0230260 MtrunA17_Chr4g0053281 42.593 108 55 1 129 229 216 323 9.17e-20 88.2
Msa0230260 MtrunA17_Chr8g0379461 46.429 84 38 1 172 255 76 152 1.24e-19 86.3
Msa0230260 MtrunA17_Chr5g0401821 45.783 83 42 1 150 229 174 256 1.98e-19 86.3
Msa0230260 MtrunA17_Chr7g0270051 40.171 117 57 2 123 232 3 113 2.51e-19 85.9
Msa0230260 MtrunA17_Chr2g0284321 35.714 154 90 5 144 290 2 153 6.47e-19 83.6
Msa0230260 MtrunA17_Chr2g0319961 57.377 61 26 0 172 232 80 140 1.73e-18 83.6
Msa0230260 MtrunA17_Chr1g0184691 53.968 63 29 0 169 231 290 352 3.07e-18 84.0
Msa0230260 MtrunA17_Chr8g0368721 48.387 93 47 1 62 153 220 312 7.29e-18 82.4
Msa0230260 MtrunA17_Chr7g0228941 50.769 65 32 0 166 230 46 110 8.71e-18 78.2
Msa0230260 MtrunA17_Chr1g0147881 43.210 81 45 1 149 229 240 319 1.47e-17 82.0
Msa0230260 MtrunA17_Chr3g0139021 42.683 82 46 1 151 231 292 373 4.45e-17 81.3
Msa0230260 MtrunA17_Chr4g0074001 52.381 63 29 1 170 232 357 418 5.82e-17 81.3
Msa0230260 MtrunA17_Chr7g0263531 52.632 76 34 2 168 243 185 258 5.08e-16 78.2
Msa0230260 MtrunA17_Chr7g0263531 42.424 66 37 1 167 232 356 420 2.54e-14 73.2
Msa0230260 MtrunA17_Chr7g0218081 47.222 72 37 1 161 232 399 469 8.55e-16 77.8
Msa0230260 MtrunA17_Chr7g0218081 46.970 66 33 2 168 233 224 287 2.78e-13 70.1
Msa0230260 MtrunA17_Chr5g0445311 43.056 72 40 1 161 232 353 423 2.10e-15 76.6
Msa0230260 MtrunA17_Chr5g0445311 44.444 72 38 2 171 242 199 268 2.62e-12 67.4
Msa0230260 MtrunA17_Chr8g0366921 46.875 96 49 2 59 153 226 320 2.48e-15 75.5
Msa0230260 MtrunA17_Chr3g0079691 41.758 91 49 3 171 260 279 366 9.85e-15 74.7
Msa0230260 MtrunA17_Chr3g0079691 35.354 99 52 2 145 232 457 554 1.21e-12 68.6
Msa0230260 MtrunA17_Chr3g0101131 41.667 72 41 1 161 232 404 474 1.01e-14 74.7
Msa0230260 MtrunA17_Chr3g0101131 45.946 74 38 2 168 241 239 310 2.84e-13 70.1
Msa0230260 MtrunA17_Chr5g0447701 48.485 66 33 1 167 232 247 311 2.11e-14 73.2
Msa0230260 MtrunA17_Chr3g0099641 31.387 137 83 2 113 249 203 328 2.32e-14 73.2
Msa0230260 MtrunA17_Chr2g0302391 44.615 65 36 0 172 236 211 275 2.88e-14 72.8
Msa0230260 MtrunA17_Chr5g0418191 38.889 90 54 1 161 250 309 397 4.10e-14 72.4
Msa0230260 MtrunA17_Chr5g0418191 35.484 124 71 5 168 285 147 267 6.65e-12 65.9
Msa0230260 MtrunA17_Chr3g0136961 45.614 57 31 0 173 229 139 195 9.19e-14 70.5
Msa0230260 MtrunA17_Chr2g0322391 36.036 111 66 2 139 249 321 426 9.26e-14 71.6
Msa0230260 MtrunA17_Chr5g0432161 48.333 60 30 1 173 232 422 480 9.79e-14 71.6
Msa0230260 MtrunA17_Chr5g0432161 52.381 63 28 2 171 233 243 303 8.58e-13 68.9
Msa0230260 MtrunA17_Chr4g0075201 31.298 131 81 3 108 232 200 327 1.21e-13 71.2
Msa0230260 MtrunA17_Chr7g0270191 36.170 94 56 3 142 232 112 204 1.83e-13 69.7
Msa0230260 MtrunA17_Chr1g0194481 36.667 90 55 2 152 241 139 226 2.16e-13 69.7
Msa0230260 MtrunA17_Chr7g0249311 30.328 122 70 3 130 237 336 456 2.27e-13 70.5
Msa0230260 MtrunA17_Chr7g0249311 45.205 73 38 2 171 243 178 248 3.95e-11 63.9
Msa0230260 MtrunA17_Chr4g0001361 42.188 64 37 0 173 236 163 226 2.29e-13 69.7
Msa0230260 MtrunA17_Chr3g0106491 43.750 64 35 1 169 232 362 424 3.87e-13 69.7
Msa0230260 MtrunA17_Chr3g0106491 51.613 62 28 2 171 232 197 256 2.23e-12 67.4
Msa0230260 MtrunA17_Chr4g0042831 43.750 64 35 1 169 232 275 337 4.52e-13 69.3
Msa0230260 MtrunA17_Chr4g0042831 33.010 103 61 2 134 236 114 208 2.78e-11 63.9
Msa0230260 MtrunA17_Chr1g0150271 27.778 162 100 4 78 234 14 163 4.66e-13 67.8
Msa0230260 MtrunA17_Chr7g0270831 38.462 78 47 1 155 232 200 276 4.91e-13 69.3
Msa0230260 MtrunA17_Chr7g0238921 40.698 86 45 3 159 244 113 192 4.96e-13 68.6
Msa0230260 MtrunA17_Chr4g0025061 36.145 83 53 0 167 249 355 437 5.78e-13 69.3
Msa0230260 MtrunA17_Chr2g0288871 35.000 100 61 3 134 232 269 365 7.55e-13 68.9
Msa0230260 MtrunA17_Chr4g0025051 36.145 83 53 0 167 249 330 412 1.64e-12 67.8
Msa0230260 MtrunA17_Chr7g0249301 47.541 61 29 3 173 232 293 351 2.08e-12 67.0
Msa0230260 MtrunA17_Chr7g0266611 63.077 65 23 1 247 311 102 165 2.45e-12 64.3
Msa0230260 MtrunA17_Chr7g0243891 47.143 70 35 2 164 233 208 275 3.17e-12 67.0
Msa0230260 MtrunA17_Chr1g0150281 43.860 57 32 0 173 229 141 197 3.29e-12 65.9
Msa0230260 MtrunA17_Chr4g0004911 42.424 66 37 1 167 232 331 395 4.29e-12 66.6
Msa0230260 MtrunA17_Chr4g0060271 38.462 78 46 1 157 232 238 315 8.70e-12 65.9
Msa0230260 MtrunA17_Chr2g0294231 45.000 60 32 1 173 232 204 262 9.08e-12 65.1
Msa0230260 MtrunA17_Chr4g0051881 41.429 70 39 2 168 237 175 242 1.04e-11 64.7
Msa0230260 MtrunA17_Chr2g0333851 37.805 82 47 1 157 234 150 231 1.05e-11 64.7
Msa0230260 MtrunA17_Chr1g0150801 44.262 61 33 1 169 229 99 158 1.27e-11 62.0
Msa0230260 MtrunA17_Chr7g0216891 31.858 113 72 3 117 229 169 276 1.38e-11 65.1
Msa0230260 MtrunA17_Chr3g0129321 40.625 64 37 1 169 232 115 177 2.42e-11 62.0
Msa0230260 MtrunA17_Chr5g0443131 41.538 65 37 1 173 237 158 221 2.72e-11 63.5
Msa0230260 MtrunA17_Chr5g0418781 34.314 102 65 2 128 229 81 180 2.96e-11 63.2
Msa0230260 MtrunA17_Chr1g0188941 32.184 87 54 2 146 232 29 110 3.36e-11 59.7
Msa0230260 MtrunA17_Chr4g0068541 37.681 69 42 1 173 241 209 276 3.68e-11 63.5
Msa0230260 MtrunA17_Chr4g0051181 41.667 60 35 0 173 232 244 303 3.70e-11 63.5
Msa0230260 MtrunA17_Chr8g0383251 42.424 66 37 1 167 232 103 167 6.43e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0230260 AT5G52830.1 51.020 98 46 2 149 246 143 238 6.13e-26 105
Msa0230260 AT4G01250.1 32.850 207 126 6 112 310 72 273 2.96e-25 103
Msa0230260 AT5G45050.2 38.983 118 70 2 149 265 1128 1244 2.00e-19 89.4
Msa0230260 AT5G45050.1 38.983 118 70 2 149 265 1156 1272 2.05e-19 89.4
Msa0230260 AT5G45050.4 38.983 118 70 2 149 265 1017 1133 2.10e-19 89.4
Msa0230260 AT2G34830.1 42.308 104 49 3 147 241 182 283 4.14e-19 87.4
Msa0230260 AT2G34830.2 42.308 104 49 3 147 241 191 292 5.09e-19 87.4
Msa0230260 AT4G23550.1 41.758 91 52 1 156 246 124 213 6.60e-19 85.9
Msa0230260 AT2G24570.1 53.968 63 29 0 169 231 240 302 8.90e-19 85.5
Msa0230260 AT4G31550.2 55.738 61 27 0 169 229 242 302 1.24e-18 85.1
Msa0230260 AT4G31550.1 55.738 61 27 0 169 229 243 303 1.24e-18 85.1
Msa0230260 AT1G29280.1 45.055 91 41 1 151 232 44 134 1.27e-18 84.0
Msa0230260 AT5G28650.1 47.059 85 42 1 150 231 237 321 1.39e-18 85.1
Msa0230260 AT1G30650.1 42.308 104 49 3 147 241 184 285 1.51e-18 85.9
Msa0230260 AT2G23320.1 57.895 57 24 0 173 229 241 297 1.53e-18 84.7
Msa0230260 AT3G04670.1 47.059 85 42 1 150 231 237 321 2.42e-18 84.3
Msa0230260 AT4G24240.1 45.783 83 42 1 150 229 256 338 3.02e-18 84.3
Msa0230260 AT3G58710.1 51.515 66 32 0 172 237 71 136 1.65e-17 81.3
Msa0230260 AT3G58710.2 51.515 66 32 0 172 237 70 135 1.74e-17 81.3
Msa0230260 AT4G30935.1 33.742 163 90 5 71 232 245 390 7.78e-17 81.3
Msa0230260 AT5G07100.1 35.294 136 80 5 100 232 163 293 8.40e-17 79.7
Msa0230260 AT5G07100.1 43.056 72 40 1 165 236 110 180 7.39e-12 65.5
Msa0230260 AT5G07100.2 35.294 136 80 5 100 232 70 200 9.47e-17 78.2
Msa0230260 AT5G07100.2 43.056 72 40 1 165 236 17 87 1.49e-11 63.5
Msa0230260 AT5G07100.4 35.294 136 80 5 100 232 70 200 9.47e-17 78.2
Msa0230260 AT5G07100.4 43.056 72 40 1 165 236 17 87 1.49e-11 63.5
Msa0230260 AT5G07100.5 35.294 136 80 5 100 232 70 200 9.47e-17 78.2
Msa0230260 AT5G07100.5 43.056 72 40 1 165 236 17 87 1.49e-11 63.5
Msa0230260 AT5G07100.3 35.294 136 80 5 100 232 70 200 9.47e-17 78.2
Msa0230260 AT5G07100.3 43.056 72 40 1 165 236 17 87 1.49e-11 63.5
Msa0230260 AT2G30590.1 52.381 63 30 0 169 231 310 372 1.67e-16 79.7
Msa0230260 AT2G04880.1 35.115 131 66 4 131 248 253 377 1.04e-15 77.8
Msa0230260 AT2G04880.2 35.115 131 66 4 131 248 229 353 1.17e-15 77.4
Msa0230260 AT3G01080.2 48.485 66 33 1 167 232 339 403 1.47e-15 77.4
Msa0230260 AT3G01080.2 45.714 70 36 2 168 237 201 268 7.19e-14 72.4
Msa0230260 AT3G01080.1 48.485 66 33 1 167 232 301 365 1.78e-15 77.0
Msa0230260 AT3G01080.1 45.714 70 36 2 168 237 163 230 8.84e-14 72.0
Msa0230260 AT4G26640.7 41.667 72 41 1 161 232 330 400 1.15e-14 74.7
Msa0230260 AT4G26640.7 34.146 123 71 3 127 249 134 246 4.78e-12 67.0
Msa0230260 AT4G26640.5 41.667 72 41 1 161 232 194 264 1.28e-14 74.3
Msa0230260 AT4G26640.5 34.454 119 68 3 131 249 2 110 1.35e-11 65.1
Msa0230260 AT4G26640.6 41.667 72 41 1 161 232 194 264 1.28e-14 74.3
Msa0230260 AT4G26640.6 34.454 119 68 3 131 249 2 110 1.35e-11 65.1
Msa0230260 AT4G26640.4 41.667 72 41 1 161 232 194 264 1.28e-14 74.3
Msa0230260 AT4G26640.4 34.454 119 68 3 131 249 2 110 1.35e-11 65.1
Msa0230260 AT4G26640.3 41.667 72 41 1 161 232 194 264 1.28e-14 74.3
Msa0230260 AT4G26640.3 34.454 119 68 3 131 249 2 110 1.35e-11 65.1
Msa0230260 AT4G26640.2 41.667 72 41 1 161 232 370 440 1.32e-14 74.7
Msa0230260 AT4G26640.2 34.146 123 71 3 127 249 174 286 5.42e-12 66.6
Msa0230260 AT4G26640.1 41.667 72 41 1 161 232 298 368 1.51e-14 74.3
Msa0230260 AT4G26640.1 34.146 123 71 3 127 249 102 214 6.84e-12 66.2
Msa0230260 AT1G69810.2 43.590 78 43 1 167 243 140 217 1.97e-14 73.2
Msa0230260 AT1G69810.1 43.590 78 43 1 167 243 198 275 2.35e-14 73.6
Msa0230260 AT2G37260.1 41.463 82 43 2 152 229 325 405 2.88e-14 73.2
Msa0230260 AT2G37260.1 50.769 65 30 2 168 232 161 223 4.26e-12 66.6
Msa0230260 AT3G01080.3 45.714 70 36 2 168 237 201 268 3.80e-14 72.4
Msa0230260 AT1G13960.1 43.939 66 36 1 167 232 404 468 4.12e-14 73.2
Msa0230260 AT1G13960.1 44.928 69 36 2 168 236 225 291 1.82e-12 68.2
Msa0230260 AT2G03340.1 43.939 66 36 1 167 232 410 474 4.23e-14 73.2
Msa0230260 AT2G03340.1 35.484 124 63 4 168 279 246 364 1.65e-12 68.2
Msa0230260 AT2G37260.3 41.463 82 43 2 152 229 245 325 4.55e-14 72.4
Msa0230260 AT2G37260.3 50.769 65 30 2 168 232 81 143 6.81e-12 65.9
Msa0230260 AT2G37260.2 41.463 82 43 2 152 229 243 323 4.89e-14 72.0
Msa0230260 AT2G37260.2 50.769 65 30 2 168 232 79 141 7.54e-12 65.9
Msa0230260 AT2G38470.1 51.471 68 31 2 166 233 178 243 5.88e-14 72.8
Msa0230260 AT2G38470.1 46.667 60 31 1 173 232 363 421 1.38e-13 71.6
Msa0230260 AT1G13960.2 43.939 66 36 1 167 232 251 315 8.70e-14 71.6
Msa0230260 AT1G13960.2 44.928 69 36 2 168 236 72 138 4.43e-12 66.6
Msa0230260 AT4G04450.2 36.145 83 53 0 167 249 193 275 2.11e-13 70.9
Msa0230260 AT5G26170.1 47.619 63 32 1 167 229 108 169 2.69e-13 67.4
Msa0230260 AT4G04450.1 36.145 83 53 0 167 249 287 369 4.44e-13 70.1
Msa0230260 AT2G30250.1 45.312 64 34 1 173 236 329 391 6.17e-13 69.3
Msa0230260 AT4G22070.4 39.394 66 40 0 167 232 292 357 6.60e-13 69.3
Msa0230260 AT4G22070.3 39.394 66 40 0 167 232 292 357 6.60e-13 69.3
Msa0230260 AT4G22070.2 39.394 66 40 0 167 232 292 357 6.60e-13 69.3
Msa0230260 AT4G22070.1 39.394 66 40 0 167 232 292 357 6.60e-13 69.3
Msa0230260 AT1G80840.1 37.805 82 47 1 158 235 128 209 7.60e-13 68.2
Msa0230260 AT4G26440.1 38.542 96 53 3 160 255 170 259 8.18e-13 69.3
Msa0230260 AT2G47260.1 36.667 90 55 2 143 232 146 233 1.00e-12 68.2
Msa0230260 AT1G62300.1 40.984 61 36 0 172 232 312 372 2.20e-12 67.8
Msa0230260 AT5G43290.1 51.667 60 28 1 170 229 112 170 3.71e-12 65.9
Msa0230260 AT1G69310.4 37.805 82 49 2 149 230 125 204 6.50e-12 65.5
Msa0230260 AT1G69310.2 37.805 82 49 2 149 230 125 204 6.50e-12 65.5
Msa0230260 AT1G69310.1 37.805 82 49 2 149 230 125 204 6.50e-12 65.5
Msa0230260 AT1G55600.1 38.356 73 43 2 161 232 295 366 7.29e-12 66.2
Msa0230260 AT4G18170.1 36.047 86 53 2 152 237 153 236 7.69e-12 65.5
Msa0230260 AT5G56270.1 43.333 60 33 1 173 232 488 546 8.61e-12 66.2
Msa0230260 AT5G56270.1 50.000 60 28 2 173 232 274 331 1.12e-11 65.9
Msa0230260 AT4G12020.5 33.333 129 68 6 168 284 397 519 1.13e-11 66.2
Msa0230260 AT4G12020.11 33.333 129 68 6 168 284 464 586 1.15e-11 66.2
Msa0230260 AT4G31800.2 40.000 60 36 0 173 232 112 171 1.16e-11 64.3
Msa0230260 AT4G12020.4 33.333 129 68 6 168 284 464 586 1.36e-11 65.9
Msa0230260 AT1G29860.1 38.272 81 47 2 162 240 124 203 1.36e-11 64.3
Msa0230260 AT4G12020.10 33.333 129 68 6 168 284 464 586 1.61e-11 65.9
Msa0230260 AT4G12020.7 33.333 129 68 6 168 284 464 586 1.66e-11 65.9
Msa0230260 AT4G31800.1 40.000 60 36 0 173 232 177 236 1.66e-11 64.3
Msa0230260 AT4G12020.6 33.333 129 68 6 168 284 464 586 1.68e-11 65.9
Msa0230260 AT4G12020.8 33.333 129 68 6 168 284 464 586 1.68e-11 65.9
Msa0230260 AT4G12020.1 33.333 129 68 6 168 284 464 586 1.68e-11 65.9
Msa0230260 AT4G12020.9 33.333 129 68 6 168 284 464 586 1.68e-11 65.9
Msa0230260 AT4G12020.3 33.333 129 68 6 168 284 464 586 1.69e-11 65.9
Msa0230260 AT4G12020.2 33.333 129 68 6 168 284 464 586 1.70e-11 65.5
Msa0230260 AT3G62340.1 40.000 85 47 3 149 232 96 177 1.84e-11 63.9
Msa0230260 AT5G49520.1 37.179 78 47 2 155 232 205 280 2.10e-11 64.7
Msa0230260 AT1G18860.1 43.333 60 34 0 173 232 240 299 2.47e-11 64.7
Msa0230260 AT5G15130.2 37.662 77 46 1 158 232 169 245 3.20e-11 64.3
Msa0230260 AT5G15130.1 37.662 77 46 1 158 232 211 287 3.77e-11 64.3
Msa0230260 AT5G41570.1 31.183 93 61 2 139 229 63 154 6.34e-11 60.8
Msa0230260 AT1G66550.2 36.364 110 59 4 149 253 78 181 8.74e-11 61.6

Find 62 sgRNAs with CRISPR-Local

Find 264 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTCTGAAACAGAATTTAT+TGG 0.120089 2_2:-2302426 Msa0230260:CDS
TAGAAACCCAAACTAGTTCT+AGG 0.250090 2_2:-2304007 Msa0230260:CDS
ACAAAGTTCTTAATTTCTTC+TGG 0.267372 2_2:+2302540 None:intergenic
GTAAAACCCTAGAACTAGTT+TGG 0.270284 2_2:+2304000 Msa0230270:intron
AAGGTGGTGGTAGTGGTAAT+TGG 0.290628 2_2:+2304317 Msa0230270:CDS
CTAGTTTGGGTTTCTAGAAT+TGG 0.312695 2_2:+2304014 Msa0230270:intron
CAAACCTCAGATCAAGAAAA+AGG 0.317679 2_2:-2303956 Msa0230260:intron
AGGTGGTGGTAGTGGTAATT+GGG 0.338809 2_2:+2304318 Msa0230270:CDS
GGTAATTGGGGTAATGATAG+TGG 0.346701 2_2:+2304331 Msa0230270:CDS
GGGAATGTGAATAGCGGTGG+TGG 0.376751 2_2:+2304376 Msa0230270:CDS
GCTTATATTGTGACGTATAA+AGG 0.379319 2_2:-2302582 Msa0230260:CDS
GATTGTTGCTGATTTGTGTT+TGG 0.383802 2_2:+2303978 Msa0230270:intron
TCTGACATAGCTGTTATGTT+TGG 0.394725 2_2:+2302372 None:intergenic
TAAAACCCTAGAACTAGTTT+GGG 0.406708 2_2:+2304001 Msa0230270:intron
ACTAACACTAGGATATTGAT+TGG 0.429065 2_2:+2304199 Msa0230270:intron
GAAATTAAGAACTTTGTTGT+TGG 0.434538 2_2:-2302534 Msa0230260:CDS
TATGTCAGAAGATTTATTGA+TGG 0.435423 2_2:-2302358 Msa0230260:CDS
TGTGTATGTTGTGGACTAGA+TGG 0.450832 2_2:+2304053 Msa0230270:intron
GACTTTAACCACCTCAATAG+TGG 0.457259 2_2:-2302336 Msa0230260:CDS
GGAATGTGAATAGCGGTGGT+GGG 0.459869 2_2:+2304377 Msa0230270:CDS
ACCTGAGAATTGCTAGTCTC+TGG 0.471222 2_2:+2302474 None:intergenic
GCCAGAGACTAGCAATTCTC+AGG 0.477878 2_2:-2302475 Msa0230260:CDS
AGCGGTTGTGATTTCAATGC+AGG 0.486927 2_2:+2304100 Msa0230270:intron
ATAACACACCAAGGACATCT+TGG 0.504754 2_2:+2303791 None:intergenic
TTGCTTCTTTGTGTATGTTG+TGG 0.506190 2_2:+2304044 Msa0230270:intron
AGAAACCCAAACTAGTTCTA+GGG 0.507577 2_2:-2304006 Msa0230260:CDS
TGTCCTTGGTGTGTTATATG+AGG 0.509380 2_2:-2303785 Msa0230260:CDS
GTTAGCGAAGGTGGTGGTAG+TGG 0.510257 2_2:+2304310 Msa0230270:CDS
CAAGGTGCTAATAGCGTTGG+CGG 0.513265 2_2:+2304286 Msa0230270:CDS
GTTGGCGGGGTTAGCGAAGG+TGG 0.515885 2_2:+2304301 Msa0230270:CDS
GAATGTGAATAGCGGTGGTG+GGG 0.524266 2_2:+2304378 Msa0230270:CDS
GGTAGGAGTAGTGGCAGTCA+AGG 0.527115 2_2:+2304268 Msa0230270:CDS
GGAATAAAGCACCACTATTG+AGG 0.537769 2_2:+2302325 None:intergenic
GGCGGGGTTAGCGAAGGTGG+TGG 0.538342 2_2:+2304304 Msa0230270:CDS
AAGGTGCTAATAGCGTTGGC+GGG 0.545221 2_2:+2304287 Msa0230270:CDS
GGTGGTGAGAGTAGTGATGG+TGG 0.545701 2_2:+2304355 Msa0230270:CDS
GATAGTGTTGGCAGTAGAGA+TGG 0.547801 2_2:+2304247 Msa0230270:CDS
GTAATTGGGGTAATGATAGT+GGG 0.548235 2_2:+2304332 Msa0230270:CDS
AAAGAATGCCAAGATGTCCT+TGG 0.553216 2_2:-2303799 Msa0230260:CDS
AGTAGAGATGGTAGGAGTAG+TGG 0.558152 2_2:+2304259 Msa0230270:CDS
TATGTTGTGGACTAGATGGA+TGG 0.570891 2_2:+2304057 Msa0230270:intron
TGAACTTTCAACAGACTCAT+GGG 0.572196 2_2:-2303757 Msa0230260:CDS
TTAAAGGGTCTCCACATCCA+AGG 0.575832 2_2:-2303707 Msa0230260:intron
GTGGTGAGAGTAGTGATGGT+GGG 0.578827 2_2:+2304356 Msa0230270:CDS
ATGTATGAAAACCTTGGATG+TGG 0.583670 2_2:+2303696 None:intergenic
ATGAACTTTCAACAGACTCA+TGG 0.586902 2_2:-2303758 Msa0230260:CDS
GTGTTGGCAGTAGAGATGGT+AGG 0.592781 2_2:+2304251 Msa0230270:CDS
AGTCAAGGTGCTAATAGCGT+TGG 0.600403 2_2:+2304283 Msa0230270:CDS
GGTGGGAATGTGAATAGCGG+TGG 0.610840 2_2:+2304373 Msa0230270:CDS
TCATGGGCATGGAGTAAACA+TGG 0.611520 2_2:-2303741 Msa0230260:CDS
AGTGTTCGTCATGATAGTGT+TGG 0.615034 2_2:+2304235 Msa0230270:intron
AATTGGGGTAATGATAGTGG+GGG 0.618091 2_2:+2304334 Msa0230270:CDS
TAATTGGGGTAATGATAGTG+GGG 0.618793 2_2:+2304333 Msa0230270:CDS
TTTCAACAGACTCATGGGCA+TGG 0.620196 2_2:-2303752 Msa0230260:CDS
AGCGTTGGCGGGGTTAGCGA+AGG 0.628213 2_2:+2304298 Msa0230270:CDS
GGTGGTGGTAGTGGTAATTG+GGG 0.635605 2_2:+2304319 Msa0230270:CDS
TTCCCTCATATAACACACCA+AGG 0.662868 2_2:+2303782 None:intergenic
ATAAAGCACCACTATTGAGG+TGG 0.665488 2_2:+2302328 None:intergenic
GTCCTTGGTGTGTTATATGA+GGG 0.673519 2_2:-2303784 Msa0230260:CDS
AGGTGCTAATAGCGTTGGCG+GGG 0.696052 2_2:+2304288 Msa0230270:CDS
GATGGTGGGAATGTGAATAG+CGG 0.710352 2_2:+2304370 Msa0230270:CDS
GTATGCACGATCAAAATGAG+CGG 0.723045 2_2:+2304082 Msa0230270:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAATAAAAAATATATAT+AGG - chr2_2:2303458-2303477 Msa0230260:intron 0.0%
!! ATAAAATAAAAAATATATAT+AGG - chr2_2:2303458-2303477 Msa0230260:intron 0.0%
!!! TTTATTTTATTTTTTGTCTT+TGG + chr2_2:2303448-2303467 None:intergenic 10.0%
!!! TTTATTTTATTTTTTGTCTT+TGG + chr2_2:2303448-2303467 None:intergenic 10.0%
!!! ATAGAAAAAGTAATTTTACA+GGG + chr2_2:2303208-2303227 None:intergenic 15.0%
!!! AATAGAAAAAGTAATTTTAC+AGG + chr2_2:2303209-2303228 None:intergenic 15.0%
!!! TCAATTTCAATTTTTTTCTT+AGG + chr2_2:2303283-2303302 None:intergenic 15.0%
!!! ATTTTAATCCTTATTTTGAT+GGG - chr2_2:2303317-2303336 Msa0230260:intron 15.0%
!! ATTAAATATTCTACATATAG+AGG - chr2_2:2303345-2303364 Msa0230260:intron 15.0%
!! TTAATATGAATAATATTTGC+AGG - chr2_2:2303376-2303395 Msa0230260:intron 15.0%
!! TAATATGAATAATATTTGCA+GGG - chr2_2:2303377-2303396 Msa0230260:intron 15.0%
!!! ATAGAAAAAGTAATTTTACA+GGG + chr2_2:2303208-2303227 None:intergenic 15.0%
!!! AATAGAAAAAGTAATTTTAC+AGG + chr2_2:2303209-2303228 None:intergenic 15.0%
!!! TCAATTTCAATTTTTTTCTT+AGG + chr2_2:2303283-2303302 None:intergenic 15.0%
!!! ATTTTAATCCTTATTTTGAT+GGG - chr2_2:2303317-2303336 Msa0230260:intron 15.0%
!! ATTAAATATTCTACATATAG+AGG - chr2_2:2303345-2303364 Msa0230260:intron 15.0%
!! TTAATATGAATAATATTTGC+AGG - chr2_2:2303376-2303395 Msa0230260:intron 15.0%
!! TAATATGAATAATATTTGCA+GGG - chr2_2:2303377-2303396 Msa0230260:intron 15.0%
!!! TTTTTTCTGTAAGAAAAGAA+AGG + chr2_2:2302896-2302915 None:intergenic 20.0%
!! ATGGACAAAAATTCATTAAA+GGG - chr2_2:2302990-2303009 Msa0230260:intron 20.0%
!!! CATTTTAATCCTTATTTTGA+TGG - chr2_2:2303316-2303335 Msa0230260:intron 20.0%
!! TAAAGTGAAGATATAAACTT+AGG + chr2_2:2303585-2303604 None:intergenic 20.0%
!! TGATAAACAAACAATTGATA+CGG - chr2_2:2303618-2303637 Msa0230260:intron 20.0%
!!! TTTTTTCTGTAAGAAAAGAA+AGG + chr2_2:2302896-2302915 None:intergenic 20.0%
!! ATGGACAAAAATTCATTAAA+GGG - chr2_2:2302990-2303009 Msa0230260:intron 20.0%
!!! CATTTTAATCCTTATTTTGA+TGG - chr2_2:2303316-2303335 Msa0230260:intron 20.0%
!! TAAAGTGAAGATATAAACTT+AGG + chr2_2:2303585-2303604 None:intergenic 20.0%
!! TGATAAACAAACAATTGATA+CGG - chr2_2:2303618-2303637 Msa0230260:intron 20.0%
! GTAAGAAAAGAAAGGTAAAA+TGG + chr2_2:2302888-2302907 None:intergenic 25.0%
! CATGGACAAAAATTCATTAA+AGG - chr2_2:2302989-2303008 Msa0230260:intron 25.0%
!! TTTTGAATGTATGAAAACCT+TGG + chr2_2:2303025-2303044 None:intergenic 25.0%
!! AAATAGTGACAGTAAACTTT+TGG - chr2_2:2303130-2303149 Msa0230260:intron 25.0%
!!! AATTTTAGCCCATCAAAATA+AGG + chr2_2:2303328-2303347 None:intergenic 25.0%
!! CTTATATAAAGGTATGCAAT+CGG + chr2_2:2303733-2303752 None:intergenic 25.0%
! ATATCAGTGCTCTTATATAA+AGG + chr2_2:2303744-2303763 None:intergenic 25.0%
! TTAATCATTCTTTGCAATCT+TGG + chr2_2:2303841-2303860 None:intergenic 25.0%
!! TGATTTGTATCTTTAACAGT+AGG - chr2_2:2303868-2303887 Msa0230260:intron 25.0%
! TCTTTAACAGTAGGAAAATA+CGG - chr2_2:2303877-2303896 Msa0230260:intron 25.0%
!! GAAATCTATGAATGATTTGT+AGG - chr2_2:2304032-2304051 Msa0230260:CDS 25.0%
!!! TTCTCTACATATTTTTTTGC+TGG + chr2_2:2304091-2304110 Msa0230270:intron 25.0%
!!! TCTTAATTTCTTCTGGTTTT+TGG + chr2_2:2304168-2304187 Msa0230270:intron 25.0%
! ACAAAGTTCTTAATTTCTTC+TGG + chr2_2:2304175-2304194 Msa0230270:intron 25.0%
!! GAAATTAAGAACTTTGTTGT+TGG - chr2_2:2304178-2304197 Msa0230260:CDS 25.0%
!!! ACAAGAGATTTTTGATTCAT+CGG + chr2_2:2304269-2304288 Msa0230270:CDS 25.0%
!!! TGTTCTGAAACAGAATTTAT+TGG - chr2_2:2304286-2304305 Msa0230260:CDS 25.0%
! TATGTCAGAAGATTTATTGA+TGG - chr2_2:2304354-2304373 Msa0230260:CDS 25.0%
! GTAAGAAAAGAAAGGTAAAA+TGG + chr2_2:2302888-2302907 None:intergenic 25.0%
! CATGGACAAAAATTCATTAA+AGG - chr2_2:2302989-2303008 Msa0230260:intron 25.0%
!! TTTTGAATGTATGAAAACCT+TGG + chr2_2:2303025-2303044 None:intergenic 25.0%
!! AAATAGTGACAGTAAACTTT+TGG - chr2_2:2303130-2303149 Msa0230260:intron 25.0%
!!! AATTTTAGCCCATCAAAATA+AGG + chr2_2:2303328-2303347 None:intergenic 25.0%
!! CTTATATAAAGGTATGCAAT+CGG + chr2_2:2303733-2303752 None:intergenic 25.0%
! ATATCAGTGCTCTTATATAA+AGG + chr2_2:2303744-2303763 None:intergenic 25.0%
! TTAATCATTCTTTGCAATCT+TGG + chr2_2:2303841-2303860 None:intergenic 25.0%
!! TGATTTGTATCTTTAACAGT+AGG - chr2_2:2303868-2303887 Msa0230260:intron 25.0%
! TCTTTAACAGTAGGAAAATA+CGG - chr2_2:2303877-2303896 Msa0230260:intron 25.0%
!! GAAATCTATGAATGATTTGT+AGG - chr2_2:2304032-2304051 Msa0230260:CDS 25.0%
!!! TTCTCTACATATTTTTTTGC+TGG + chr2_2:2304091-2304110 Msa0230270:intron 25.0%
!!! TCTTAATTTCTTCTGGTTTT+TGG + chr2_2:2304168-2304187 Msa0230270:intron 25.0%
! ACAAAGTTCTTAATTTCTTC+TGG + chr2_2:2304175-2304194 Msa0230270:intron 25.0%
!! GAAATTAAGAACTTTGTTGT+TGG - chr2_2:2304178-2304197 Msa0230260:CDS 25.0%
!!! ACAAGAGATTTTTGATTCAT+CGG + chr2_2:2304269-2304288 Msa0230270:CDS 25.0%
!!! TGTTCTGAAACAGAATTTAT+TGG - chr2_2:2304286-2304305 Msa0230260:CDS 25.0%
! TATGTCAGAAGATTTATTGA+TGG - chr2_2:2304354-2304373 Msa0230260:CDS 25.0%
ACTAACACTAGGATATTGAT+TGG + chr2_2:2302516-2302535 None:intergenic 30.0%
GTTGAAAAATCACTAACACT+AGG + chr2_2:2302527-2302546 None:intergenic 30.0%
! TAAAACCCTAGAACTAGTTT+GGG + chr2_2:2302714-2302733 None:intergenic 30.0%
! ATTACCTTTTTCTTGATCTG+AGG + chr2_2:2302763-2302782 None:intergenic 30.0%
! GTTCGATGAGATTTAAGTTA+TGG + chr2_2:2302860-2302879 None:intergenic 30.0%
! CTATTTAAAACATGTGGTGT+GGG + chr2_2:2303116-2303135 None:intergenic 30.0%
! ACTATTTAAAACATGTGGTG+TGG + chr2_2:2303117-2303136 None:intergenic 30.0%
! CTGTCACTATTTAAAACATG+TGG + chr2_2:2303122-2303141 None:intergenic 30.0%
ATCCTTGCAATTTCAAAGAA+TGG - chr2_2:2303153-2303172 Msa0230260:intron 30.0%
!! TACCATTCTTTGAAATTGCA+AGG + chr2_2:2303158-2303177 None:intergenic 30.0%
TAAAAACCAAAAGCCAAAGA+TGG - chr2_2:2303415-2303434 Msa0230260:intron 30.0%
!!! AATAAACCATCTTTGGCTTT+TGG + chr2_2:2303424-2303443 None:intergenic 30.0%
! CTTTGGAAATAAACCATCTT+TGG + chr2_2:2303431-2303450 None:intergenic 30.0%
ACACATATATATGCCATGAT+GGG - chr2_2:2303488-2303507 Msa0230260:intron 30.0%
!! ATATGTTTTTGATCCCATCA+TGG + chr2_2:2303504-2303523 None:intergenic 30.0%
TTGCTCAATGGAAAAAGATA+CGG + chr2_2:2303800-2303819 None:intergenic 30.0%
! CTTTTTCCATTGAGCAATTA+AGG - chr2_2:2303803-2303822 Msa0230260:CDS 30.0%
ATGATATTCTAACAACCTAC+AGG + chr2_2:2303912-2303931 Msa0230270:intron 30.0%
GCTTATATTGTGACGTATAA+AGG - chr2_2:2304130-2304149 Msa0230260:CDS 30.0%
ACTAACACTAGGATATTGAT+TGG + chr2_2:2302516-2302535 None:intergenic 30.0%
GTTGAAAAATCACTAACACT+AGG + chr2_2:2302527-2302546 None:intergenic 30.0%
! TAAAACCCTAGAACTAGTTT+GGG + chr2_2:2302714-2302733 None:intergenic 30.0%
! ATTACCTTTTTCTTGATCTG+AGG + chr2_2:2302763-2302782 None:intergenic 30.0%
! GTTCGATGAGATTTAAGTTA+TGG + chr2_2:2302860-2302879 None:intergenic 30.0%
! CTATTTAAAACATGTGGTGT+GGG + chr2_2:2303116-2303135 None:intergenic 30.0%
! ACTATTTAAAACATGTGGTG+TGG + chr2_2:2303117-2303136 None:intergenic 30.0%
! CTGTCACTATTTAAAACATG+TGG + chr2_2:2303122-2303141 None:intergenic 30.0%
ATCCTTGCAATTTCAAAGAA+TGG - chr2_2:2303153-2303172 Msa0230260:intron 30.0%
!! TACCATTCTTTGAAATTGCA+AGG + chr2_2:2303158-2303177 None:intergenic 30.0%
TAAAAACCAAAAGCCAAAGA+TGG - chr2_2:2303415-2303434 Msa0230260:intron 30.0%
!!! AATAAACCATCTTTGGCTTT+TGG + chr2_2:2303424-2303443 None:intergenic 30.0%
! CTTTGGAAATAAACCATCTT+TGG + chr2_2:2303431-2303450 None:intergenic 30.0%
ACACATATATATGCCATGAT+GGG - chr2_2:2303488-2303507 Msa0230260:intron 30.0%
!! ATATGTTTTTGATCCCATCA+TGG + chr2_2:2303504-2303523 None:intergenic 30.0%
TTGCTCAATGGAAAAAGATA+CGG + chr2_2:2303800-2303819 None:intergenic 30.0%
! CTTTTTCCATTGAGCAATTA+AGG - chr2_2:2303803-2303822 Msa0230260:CDS 30.0%
ATGATATTCTAACAACCTAC+AGG + chr2_2:2303912-2303931 Msa0230270:intron 30.0%
GCTTATATTGTGACGTATAA+AGG - chr2_2:2304130-2304149 Msa0230260:CDS 30.0%
! TAATTGGGGTAATGATAGTG+GGG + chr2_2:2302382-2302401 None:intergenic 35.0%
GTAATTGGGGTAATGATAGT+GGG + chr2_2:2302383-2302402 None:intergenic 35.0%
TTGCTTCTTTGTGTATGTTG+TGG + chr2_2:2302671-2302690 None:intergenic 35.0%
!! CTAGTTTGGGTTTCTAGAAT+TGG + chr2_2:2302701-2302720 None:intergenic 35.0%
! TAGAAACCCAAACTAGTTCT+AGG - chr2_2:2302705-2302724 Msa0230260:intron 35.0%
!! AGAAACCCAAACTAGTTCTA+GGG - chr2_2:2302706-2302725 Msa0230260:intron 35.0%
GTAAAACCCTAGAACTAGTT+TGG + chr2_2:2302715-2302734 None:intergenic 35.0%
!! GATTGTTGCTGATTTGTGTT+TGG + chr2_2:2302737-2302756 None:intergenic 35.0%
CAAACCTCAGATCAAGAAAA+AGG - chr2_2:2302756-2302775 Msa0230260:intron 35.0%
ATGAACTTTCAACAGACTCA+TGG - chr2_2:2302954-2302973 Msa0230260:intron 35.0%
TGAACTTTCAACAGACTCAT+GGG - chr2_2:2302955-2302974 Msa0230260:intron 35.0%
ATGTATGAAAACCTTGGATG+TGG + chr2_2:2303019-2303038 None:intergenic 35.0%
CATTGCAAATATTCCACCTT+TGG - chr2_2:2303229-2303248 Msa0230260:intron 35.0%
AGGAACTATAACAAACACCA+GGG + chr2_2:2303263-2303282 None:intergenic 35.0%
TAGGAACTATAACAAACACC+AGG + chr2_2:2303264-2303283 None:intergenic 35.0%
CACACATATATATGCCATGA+TGG - chr2_2:2303487-2303506 Msa0230260:intron 35.0%
AAGAAATATGCCACTCATTG+TGG - chr2_2:2303700-2303719 Msa0230260:intron 35.0%
AGAAATATGCCACTCATTGT+GGG - chr2_2:2303701-2303720 Msa0230260:intron 35.0%
!!! TGTGGAAAGTTGTTGGTTTT+TGG + chr2_2:2303775-2303794 None:intergenic 35.0%
TTCTTTGCAATCTTGGAAAG+CGG + chr2_2:2303834-2303853 None:intergenic 35.0%
!! CAGCATCATTTTGTTATGTG+AGG + chr2_2:2304206-2304225 Msa0230270:intron 35.0%
TCTGACATAGCTGTTATGTT+TGG + chr2_2:2304343-2304362 Msa0230270:CDS 35.0%
! TAATTGGGGTAATGATAGTG+GGG + chr2_2:2302382-2302401 None:intergenic 35.0%
GTAATTGGGGTAATGATAGT+GGG + chr2_2:2302383-2302402 None:intergenic 35.0%
TTGCTTCTTTGTGTATGTTG+TGG + chr2_2:2302671-2302690 None:intergenic 35.0%
!! CTAGTTTGGGTTTCTAGAAT+TGG + chr2_2:2302701-2302720 None:intergenic 35.0%
! TAGAAACCCAAACTAGTTCT+AGG - chr2_2:2302705-2302724 Msa0230260:intron 35.0%
!! AGAAACCCAAACTAGTTCTA+GGG - chr2_2:2302706-2302725 Msa0230260:intron 35.0%
GTAAAACCCTAGAACTAGTT+TGG + chr2_2:2302715-2302734 None:intergenic 35.0%
!! GATTGTTGCTGATTTGTGTT+TGG + chr2_2:2302737-2302756 None:intergenic 35.0%
CAAACCTCAGATCAAGAAAA+AGG - chr2_2:2302756-2302775 Msa0230260:intron 35.0%
ATGAACTTTCAACAGACTCA+TGG - chr2_2:2302954-2302973 Msa0230260:intron 35.0%
TGAACTTTCAACAGACTCAT+GGG - chr2_2:2302955-2302974 Msa0230260:intron 35.0%
ATGTATGAAAACCTTGGATG+TGG + chr2_2:2303019-2303038 None:intergenic 35.0%
CATTGCAAATATTCCACCTT+TGG - chr2_2:2303229-2303248 Msa0230260:intron 35.0%
AGGAACTATAACAAACACCA+GGG + chr2_2:2303263-2303282 None:intergenic 35.0%
TAGGAACTATAACAAACACC+AGG + chr2_2:2303264-2303283 None:intergenic 35.0%
CACACATATATATGCCATGA+TGG - chr2_2:2303487-2303506 Msa0230260:intron 35.0%
AAGAAATATGCCACTCATTG+TGG - chr2_2:2303700-2303719 Msa0230260:intron 35.0%
AGAAATATGCCACTCATTGT+GGG - chr2_2:2303701-2303720 Msa0230260:intron 35.0%
!!! TGTGGAAAGTTGTTGGTTTT+TGG + chr2_2:2303775-2303794 None:intergenic 35.0%
TTCTTTGCAATCTTGGAAAG+CGG + chr2_2:2303834-2303853 None:intergenic 35.0%
!! CAGCATCATTTTGTTATGTG+AGG + chr2_2:2304206-2304225 Msa0230270:intron 35.0%
TCTGACATAGCTGTTATGTT+TGG + chr2_2:2304343-2304362 Msa0230270:CDS 35.0%
!! AATTGGGGTAATGATAGTGG+GGG + chr2_2:2302381-2302400 None:intergenic 40.0%
GGTAATTGGGGTAATGATAG+TGG + chr2_2:2302384-2302403 None:intergenic 40.0%
! AGTGTTCGTCATGATAGTGT+TGG + chr2_2:2302480-2302499 None:intergenic 40.0%
GTATGCACGATCAAAATGAG+CGG + chr2_2:2302633-2302652 None:intergenic 40.0%
!! TATGTTGTGGACTAGATGGA+TGG + chr2_2:2302658-2302677 None:intergenic 40.0%
!! TGTGTATGTTGTGGACTAGA+TGG + chr2_2:2302662-2302681 None:intergenic 40.0%
AAAGAATGCCAAGATGTCCT+TGG - chr2_2:2302913-2302932 Msa0230260:intron 40.0%
ATAACACACCAAGGACATCT+TGG + chr2_2:2302924-2302943 None:intergenic 40.0%
! TGTCCTTGGTGTGTTATATG+AGG - chr2_2:2302927-2302946 Msa0230260:intron 40.0%
! GTCCTTGGTGTGTTATATGA+GGG - chr2_2:2302928-2302947 Msa0230260:intron 40.0%
TTCCCTCATATAACACACCA+AGG + chr2_2:2302933-2302952 None:intergenic 40.0%
ATGGAAAAAGATACGGTGTG+TGG + chr2_2:2303793-2303812 None:intergenic 40.0%
TTCCATTGAGCAATTAAGGC+TGG - chr2_2:2303807-2303826 Msa0230260:intron 40.0%
TCTTTGCAATCTTGGAAAGC+GGG + chr2_2:2303833-2303852 None:intergenic 40.0%
AACAGTAGGAAAATACGGTG+TGG - chr2_2:2303882-2303901 Msa0230260:intron 40.0%
GACTTTAACCACCTCAATAG+TGG - chr2_2:2304376-2304395 Msa0230260:CDS 40.0%
! ATAAAGCACCACTATTGAGG+TGG + chr2_2:2304387-2304406 Msa0230270:CDS 40.0%
! GGAATAAAGCACCACTATTG+AGG + chr2_2:2304390-2304409 Msa0230270:CDS 40.0%
!! AATTGGGGTAATGATAGTGG+GGG + chr2_2:2302381-2302400 None:intergenic 40.0%
GGTAATTGGGGTAATGATAG+TGG + chr2_2:2302384-2302403 None:intergenic 40.0%
! AGTGTTCGTCATGATAGTGT+TGG + chr2_2:2302480-2302499 None:intergenic 40.0%
GTATGCACGATCAAAATGAG+CGG + chr2_2:2302633-2302652 None:intergenic 40.0%
!! TATGTTGTGGACTAGATGGA+TGG + chr2_2:2302658-2302677 None:intergenic 40.0%
!! TGTGTATGTTGTGGACTAGA+TGG + chr2_2:2302662-2302681 None:intergenic 40.0%
AAAGAATGCCAAGATGTCCT+TGG - chr2_2:2302913-2302932 Msa0230260:intron 40.0%
ATAACACACCAAGGACATCT+TGG + chr2_2:2302924-2302943 None:intergenic 40.0%
! TGTCCTTGGTGTGTTATATG+AGG - chr2_2:2302927-2302946 Msa0230260:intron 40.0%
! GTCCTTGGTGTGTTATATGA+GGG - chr2_2:2302928-2302947 Msa0230260:intron 40.0%
TTCCCTCATATAACACACCA+AGG + chr2_2:2302933-2302952 None:intergenic 40.0%
ATGGAAAAAGATACGGTGTG+TGG + chr2_2:2303793-2303812 None:intergenic 40.0%
TTCCATTGAGCAATTAAGGC+TGG - chr2_2:2303807-2303826 Msa0230260:intron 40.0%
TCTTTGCAATCTTGGAAAGC+GGG + chr2_2:2303833-2303852 None:intergenic 40.0%
AACAGTAGGAAAATACGGTG+TGG - chr2_2:2303882-2303901 Msa0230260:intron 40.0%
GACTTTAACCACCTCAATAG+TGG - chr2_2:2304376-2304395 Msa0230260:CDS 40.0%
! ATAAAGCACCACTATTGAGG+TGG + chr2_2:2304387-2304406 Msa0230270:CDS 40.0%
! GGAATAAAGCACCACTATTG+AGG + chr2_2:2304390-2304409 Msa0230270:CDS 40.0%
!! GATGGTGGGAATGTGAATAG+CGG + chr2_2:2302345-2302364 None:intergenic 45.0%
!! AGGTGGTGGTAGTGGTAATT+GGG + chr2_2:2302397-2302416 None:intergenic 45.0%
!! AAGGTGGTGGTAGTGGTAAT+TGG + chr2_2:2302398-2302417 None:intergenic 45.0%
! AGTCAAGGTGCTAATAGCGT+TGG + chr2_2:2302432-2302451 None:intergenic 45.0%
AGTAGAGATGGTAGGAGTAG+TGG + chr2_2:2302456-2302475 None:intergenic 45.0%
!! GATAGTGTTGGCAGTAGAGA+TGG + chr2_2:2302468-2302487 None:intergenic 45.0%
AGCGGTTGTGATTTCAATGC+AGG + chr2_2:2302615-2302634 None:intergenic 45.0%
TTTCAACAGACTCATGGGCA+TGG - chr2_2:2302960-2302979 Msa0230260:intron 45.0%
TCATGGGCATGGAGTAAACA+TGG - chr2_2:2302971-2302990 Msa0230260:intron 45.0%
TTAAAGGGTCTCCACATCCA+AGG - chr2_2:2303005-2303024 Msa0230260:intron 45.0%
CAGGGACAAAAAACCAAAGG+TGG + chr2_2:2303245-2303264 None:intergenic 45.0%
!!! CACCTTTGGTTTTTTGTCCC+TGG - chr2_2:2303243-2303262 Msa0230260:intron 45.0%
CACCAGGGACAAAAAACCAA+AGG + chr2_2:2303248-2303267 None:intergenic 45.0%
ATATGCCACTCATTGTGGGA+CGG - chr2_2:2303705-2303724 Msa0230260:intron 45.0%
TACGGTGTGTGGAAAGTTGT+TGG + chr2_2:2303782-2303801 None:intergenic 45.0%
! ACCTGAGAATTGCTAGTCTC+TGG + chr2_2:2304241-2304260 Msa0230270:CDS 45.0%
!! GATGGTGGGAATGTGAATAG+CGG + chr2_2:2302345-2302364 None:intergenic 45.0%
!! AGGTGGTGGTAGTGGTAATT+GGG + chr2_2:2302397-2302416 None:intergenic 45.0%
!! AAGGTGGTGGTAGTGGTAAT+TGG + chr2_2:2302398-2302417 None:intergenic 45.0%
! AGTCAAGGTGCTAATAGCGT+TGG + chr2_2:2302432-2302451 None:intergenic 45.0%
AGTAGAGATGGTAGGAGTAG+TGG + chr2_2:2302456-2302475 None:intergenic 45.0%
!! GATAGTGTTGGCAGTAGAGA+TGG + chr2_2:2302468-2302487 None:intergenic 45.0%
AGCGGTTGTGATTTCAATGC+AGG + chr2_2:2302615-2302634 None:intergenic 45.0%
TTTCAACAGACTCATGGGCA+TGG - chr2_2:2302960-2302979 Msa0230260:intron 45.0%
TCATGGGCATGGAGTAAACA+TGG - chr2_2:2302971-2302990 Msa0230260:intron 45.0%
TTAAAGGGTCTCCACATCCA+AGG - chr2_2:2303005-2303024 Msa0230260:intron 45.0%
CAGGGACAAAAAACCAAAGG+TGG + chr2_2:2303245-2303264 None:intergenic 45.0%
!!! CACCTTTGGTTTTTTGTCCC+TGG - chr2_2:2303243-2303262 Msa0230260:intron 45.0%
CACCAGGGACAAAAAACCAA+AGG + chr2_2:2303248-2303267 None:intergenic 45.0%
ATATGCCACTCATTGTGGGA+CGG - chr2_2:2303705-2303724 Msa0230260:intron 45.0%
TACGGTGTGTGGAAAGTTGT+TGG + chr2_2:2303782-2303801 None:intergenic 45.0%
! ACCTGAGAATTGCTAGTCTC+TGG + chr2_2:2304241-2304260 Msa0230270:CDS 45.0%
!! AATATTATTCATATTAAAAT+TGG + chr2_2:2303373-2303392 None:intergenic 5.0%
!! AATATTATTCATATTAAAAT+TGG + chr2_2:2303373-2303392 None:intergenic 5.0%
GAATGTGAATAGCGGTGGTG+GGG + chr2_2:2302337-2302356 None:intergenic 50.0%
GGAATGTGAATAGCGGTGGT+GGG + chr2_2:2302338-2302357 None:intergenic 50.0%
GTGGTGAGAGTAGTGATGGT+GGG + chr2_2:2302359-2302378 None:intergenic 50.0%
!! GGTGGTGGTAGTGGTAATTG+GGG + chr2_2:2302396-2302415 None:intergenic 50.0%
!! AAGGTGCTAATAGCGTTGGC+GGG + chr2_2:2302428-2302447 None:intergenic 50.0%
! CAAGGTGCTAATAGCGTTGG+CGG + chr2_2:2302429-2302448 None:intergenic 50.0%
! GTGTTGGCAGTAGAGATGGT+AGG + chr2_2:2302464-2302483 None:intergenic 50.0%
GTGGTGTGGGTGTGTGTATT+AGG + chr2_2:2303103-2303122 None:intergenic 50.0%
GGCCAGCCTTAATTGCTCAA+TGG + chr2_2:2303812-2303831 None:intergenic 50.0%
ATACGGTGTGGACAACCTGT+AGG - chr2_2:2303894-2303913 Msa0230260:intron 50.0%
GCCAGAGACTAGCAATTCTC+AGG - chr2_2:2304237-2304256 Msa0230260:CDS 50.0%
GAATGTGAATAGCGGTGGTG+GGG + chr2_2:2302337-2302356 None:intergenic 50.0%
GGAATGTGAATAGCGGTGGT+GGG + chr2_2:2302338-2302357 None:intergenic 50.0%
GTGGTGAGAGTAGTGATGGT+GGG + chr2_2:2302359-2302378 None:intergenic 50.0%
!! GGTGGTGGTAGTGGTAATTG+GGG + chr2_2:2302396-2302415 None:intergenic 50.0%
!! AAGGTGCTAATAGCGTTGGC+GGG + chr2_2:2302428-2302447 None:intergenic 50.0%
! CAAGGTGCTAATAGCGTTGG+CGG + chr2_2:2302429-2302448 None:intergenic 50.0%
! GTGTTGGCAGTAGAGATGGT+AGG + chr2_2:2302464-2302483 None:intergenic 50.0%
GTGGTGTGGGTGTGTGTATT+AGG + chr2_2:2303103-2303122 None:intergenic 50.0%
GGCCAGCCTTAATTGCTCAA+TGG + chr2_2:2303812-2303831 None:intergenic 50.0%
ATACGGTGTGGACAACCTGT+AGG - chr2_2:2303894-2303913 Msa0230260:intron 50.0%
GCCAGAGACTAGCAATTCTC+AGG - chr2_2:2304237-2304256 Msa0230260:CDS 50.0%
GGGAATGTGAATAGCGGTGG+TGG + chr2_2:2302339-2302358 None:intergenic 55.0%
GGTGGGAATGTGAATAGCGG+TGG + chr2_2:2302342-2302361 None:intergenic 55.0%
GGTGGTGAGAGTAGTGATGG+TGG + chr2_2:2302360-2302379 None:intergenic 55.0%
!! TGGGGTAATGATAGTGGGGG+TGG + chr2_2:2302378-2302397 None:intergenic 55.0%
! GTTAGCGAAGGTGGTGGTAG+TGG + chr2_2:2302405-2302424 None:intergenic 55.0%
!! AGGTGCTAATAGCGTTGGCG+GGG + chr2_2:2302427-2302446 None:intergenic 55.0%
GGTAGGAGTAGTGGCAGTCA+AGG + chr2_2:2302447-2302466 None:intergenic 55.0%
GTAATGTGTGCGCGCGCAAA+TGG - chr2_2:2303965-2303984 Msa0230260:CDS 55.0%
GGGAATGTGAATAGCGGTGG+TGG + chr2_2:2302339-2302358 None:intergenic 55.0%
GGTGGGAATGTGAATAGCGG+TGG + chr2_2:2302342-2302361 None:intergenic 55.0%
GGTGGTGAGAGTAGTGATGG+TGG + chr2_2:2302360-2302379 None:intergenic 55.0%
!! TGGGGTAATGATAGTGGGGG+TGG + chr2_2:2302378-2302397 None:intergenic 55.0%
! GTTAGCGAAGGTGGTGGTAG+TGG + chr2_2:2302405-2302424 None:intergenic 55.0%
!! AGGTGCTAATAGCGTTGGCG+GGG + chr2_2:2302427-2302446 None:intergenic 55.0%
GGTAGGAGTAGTGGCAGTCA+AGG + chr2_2:2302447-2302466 None:intergenic 55.0%
GTAATGTGTGCGCGCGCAAA+TGG - chr2_2:2303965-2303984 Msa0230260:CDS 55.0%
GGGGGTGGTGAGAGTAGTGA+TGG + chr2_2:2302363-2302382 None:intergenic 60.0%
CGGTTCCGTCCCACAATGAG+TGG + chr2_2:2303713-2303732 None:intergenic 60.0%
GGGGGTGGTGAGAGTAGTGA+TGG + chr2_2:2302363-2302382 None:intergenic 60.0%
CGGTTCCGTCCCACAATGAG+TGG + chr2_2:2303713-2303732 None:intergenic 60.0%
! GTTGGCGGGGTTAGCGAAGG+TGG + chr2_2:2302414-2302433 None:intergenic 65.0%
!! AGCGTTGGCGGGGTTAGCGA+AGG + chr2_2:2302417-2302436 None:intergenic 65.0%
! GTTGGCGGGGTTAGCGAAGG+TGG + chr2_2:2302414-2302433 None:intergenic 65.0%
!! AGCGTTGGCGGGGTTAGCGA+AGG + chr2_2:2302417-2302436 None:intergenic 65.0%
! GGCGGGGTTAGCGAAGGTGG+TGG + chr2_2:2302411-2302430 None:intergenic 70.0%
! GGCGGGGTTAGCGAAGGTGG+TGG + chr2_2:2302411-2302430 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 2302320 2304414 2302320 ID=Msa0230260;Name=Msa0230260
chr2_2 mRNA 2302320 2304414 2302320 ID=Msa0230260-mRNA-1;Parent=Msa0230260;Name=Msa0230260-mRNA-1;_AED=0.21;_eAED=0.21;_QI=0|0|0|1|1|1|3|0|311
chr2_2 exon 2303957 2304414 2303957 ID=Msa0230260-mRNA-1:exon:822;Parent=Msa0230260-mRNA-1
chr2_2 exon 2303708 2303824 2303708 ID=Msa0230260-mRNA-1:exon:821;Parent=Msa0230260-mRNA-1
chr2_2 exon 2302320 2302680 2302320 ID=Msa0230260-mRNA-1:exon:820;Parent=Msa0230260-mRNA-1
chr2_2 CDS 2303957 2304414 2303957 ID=Msa0230260-mRNA-1:cds;Parent=Msa0230260-mRNA-1
chr2_2 CDS 2303708 2303824 2303708 ID=Msa0230260-mRNA-1:cds;Parent=Msa0230260-mRNA-1
chr2_2 CDS 2302320 2302680 2302320 ID=Msa0230260-mRNA-1:cds;Parent=Msa0230260-mRNA-1
Gene Sequence

>Msa0230260

ATGCTCAAATTCAACCCCACCACCGCTATTCACATTCCCACCATCACTACTCTCACCACCCCCACTATCATTACCCCAATTACCACTACCACCACCTTCGCTAACCCCGCCAACGCTATTAGCACCTTGACTGCCACTACTCCTACCATCTCTACTGCCAACACTATCATGACGAACACTAGCGTTCATGAAACCAATCAATATCCTAGTGTTAGTGATTTTTCAACACTCGTTGAACAACAACATATTCATCAAAGCAACAACAATCAAGTGTTTGTTTCGAAACATGTTGCCTGCATTGAAATCACAACCGCTCATTTTGATCGTGCATACAACCATCCATCTAGTCCACAACATACACAAAGAAGCAATCAACTTCCAATTCTAGAAACCCAAACTAGTTCTAGGGTTTTACCAAACACAAATCAGCAACAATCAAACCTCAGATCAAGAAAAAGAAAAAAGAATGCCAAGATGTCCTTGGTGTGTTATATGAGGGAAGATGAACTTTCAACAGACTCATGGGCATGGAGTAAACATGGACAAAAATTCATTAAAGGGTCTCCACATCCAAGGTGCTATTATAAGTGCAGCAGTTTCGCAAATTGTCCAGCAAAAAAATATGTAGAGAAAAGCAAAACTCATGAGAACGCTTATATTGTGACGTATAAAGGAGAGCATGATCACCAAAAACCAGAAGAAATTAAGAACTTTGTTGTTGGTACCTCACATAACAAAATGATGCTGAAGTTTGATGAGCCAGAGACTAGCAATTCTCAGGTTTTTTCCGATGAATCAAAAATCTCTTGTTCTGAAACAGAATTTATTGGAAACTCTAATGATGATGATGACATTTTGATACCAAACATAACAGCTATGTCAGAAGATTTATTGATGGACTTTAACCACCTCAATAGTGGTGCTTTATTCCCATAG
Protein sequence

>Msa0230260

MLKFNPTTAIHIPTITTLTTPTIITPITTTTTFANPANAISTLTATTPTISTANTIMTNTSVHETNQYPSVSDFSTLVEQQHIHQSNNNQVFVSKHVACIEITTAHFDRAYNHPSSPQHTQRSNQLPILETQTSSRVLPNTNQQQSNLRSRKRKKNAKMSLVCYMREDELSTDSWAWSKHGQKFIKGSPHPRCYYKCSSFANCPAKKYVEKSKTHENAYIVTYKGEHDHQKPEEIKNFVVGTSHNKMMLKFDEPETSNSQVFSDESKISCSETEFIGNSNDDDDILIPNITAMSEDLLMDFNHLNSGALFP*