AlfalfaGEDB | Msa0234080

M. sativa cultivar zhongmu-4 / Msa0234080

KEGG: K17604
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0234080	XP_039685680.1	84.856	799	101	3	1	779	1	799	0.0	1392

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0234080	sp\|Q9SZL8\|FRS5_ARATH	27.925	641	382	14	32	665	70	637	1.53e-69	248

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0234080	D3YBB9	76.456	807	173	4	1	798	49	847	0.0	1283

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TFs/TRs:

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Gene ID	Type	Classification
Msa0234080	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0234080	MtrunA17_Chr3g0088371	83.152	184	28	1	618	798	1	184	8.76e-103	315
Msa0234080	MtrunA17_Chr2g0324011	30.709	635	364	12	36	665	8	571	3.05e-101	325
Msa0234080	MtrunA17_Chr3g0082121	31.693	691	384	17	41	727	49	655	1.43e-100	326
Msa0234080	MtrunA17_Chr1g0202341	29.606	635	366	17	34	662	50	609	6.25e-80	271
Msa0234080	MtrunA17_Chr8g0363841	28.776	629	375	13	40	665	22	580	5.12e-75	257
Msa0234080	MtrunA17_Chr1g0203721	26.026	780	452	20	1	739	16	711	5.73e-73	256
Msa0234080	MtrunA17_Chr2g0296781	27.688	679	405	20	39	708	1	602	1.71e-68	240
Msa0234080	MtrunA17_Chr5g0448761	25.318	707	449	14	31	728	56	692	2.00e-68	241
Msa0234080	MtrunA17_Chr6g0458391	26.984	630	357	15	41	662	231	765	1.99e-65	233
Msa0234080	MtrunA17_Chr6g0477261	29.412	527	314	14	180	699	7	482	5.96e-65	229

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0234080	AT4G38180.1	27.925	641	382	14	32	665	70	637	1.55e-70	248
Msa0234080	AT4G15090.1	25.000	696	435	15	50	741	66	678	2.07e-59	217
Msa0234080	AT4G15090.5	25.000	696	435	15	50	741	66	678	2.07e-59	217
Msa0234080	AT4G15090.3	25.000	696	435	15	50	741	66	678	2.07e-59	217
Msa0234080	AT4G15090.2	25.000	696	435	15	50	741	66	678	2.07e-59	217
Msa0234080	AT4G15090.4	25.000	696	435	15	50	741	66	678	2.07e-59	217
Msa0234080	AT2G27110.2	25.070	714	423	19	10	719	29	634	1.09e-53	200
Msa0234080	AT2G27110.1	25.070	714	423	19	10	719	29	634	1.09e-53	200
Msa0234080	AT2G27110.3	27.308	509	313	11	213	719	36	489	4.79e-53	197
Msa0234080	AT3G06250.2	25.118	633	351	16	34	661	189	703	5.24e-52	194

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Find 124 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGCTCTGGTTTATGTAA+TGG	0.504865	2_2:+6567752	None:intergenic
AACAAAGAAGGTTTCAAGAA+GGG	0.545537	2_2:-6567950	Msa0234080:CDS
AACAATAAATATCGGCCATT+AGG	0.388497	2_2:-6567350	Msa0234080:intron
AACTCGTCAAAAGGAGATGG+TGG	0.603142	2_2:-6567558	Msa0234080:CDS
AAGAAAGACCAAATCCAATT+AGG	0.397771	2_2:-6565502	Msa0234080:CDS
AATCAGATTTCGACTACAAT+TGG	0.318540	2_2:-6566582	Msa0234080:CDS
AATCGAACAAGTGAATGGTC+TGG	0.540971	2_2:+6566379	None:intergenic
AATGAAGCTCTCTCGAAACC+AGG	0.565261	2_2:-6567500	Msa0234080:CDS
AATTCTTGATTGTTATGCAT+TGG	0.467731	2_2:-6565264	Msa0234080:three_prime_UTR
ACAAAATAAGAAATCGGATA+AGG	0.469178	2_2:-6567996	Msa0234080:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATATTAACTTCATCTGAGAG+TGG	-	chr2_2:6565304-6565323	Msa0234080:three_prime_UTR	30.0%
TAAAGAATCATGGAAGTCTT+TGG	-	chr2_2:6565390-6565409	Msa0234080:exon	30.0%
CGTTATGTTTGTAACAAAGA+AGG	-	chr2_2:6565544-6565563	Msa0234080:CDS	30.0%
TAACAAAGAAGGTTTCAAGA+AGG	-	chr2_2:6565555-6565574	Msa0234080:CDS	30.0%
AACAAAGAAGGTTTCAAGAA+GGG	-	chr2_2:6565556-6565575	Msa0234080:CDS	30.0%
TAAAGAAAAACCAGAGGAAA+GGG	-	chr2_2:6565645-6565664	Msa0234080:CDS	30.0%
AGAACATCTTATGAAGTGAT+GGG	-	chr2_2:6565859-6565878	Msa0234080:CDS	30.0%
TTTAAGTCCGAAAATCGAAA+AGG	+	chr2_2:6565913-6565932	None:intergenic	30.0%
TGGATATCATAGTAAAAACC+TGG	+	chr2_2:6566027-6566046	None:intergenic	30.0%
CAACAAGATTATGCTCTATT+TGG	-	chr2_2:6566099-6566118	Msa0234080:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_2	gene	6565245	6568283	6565245	ID=Msa0234080;Name=Msa0234080
chr2_2	mRNA	6565245	6568283	6565245	ID=Msa0234080-mRNA-1;Parent=Msa0234080;Name=Msa0234080-mRNA-1;_AED=0.00;_eAED=0.00;_QI=50\|1\|1\|1\|1\|1\|2\|152\|800
chr2_2	exon	6565245	6566916	6565245	ID=Msa0234080-mRNA-1:exon:2906;Parent=Msa0234080-mRNA-1
chr2_2	exon	6567351	6568283	6567351	ID=Msa0234080-mRNA-1:exon:2905;Parent=Msa0234080-mRNA-1
chr2_2	five_prime_UTR	6568234	6568283	6568234	ID=Msa0234080-mRNA-1:five_prime_utr;Parent=Msa0234080-mRNA-1
chr2_2	CDS	6567351	6568233	6567351	ID=Msa0234080-mRNA-1:cds;Parent=Msa0234080-mRNA-1
chr2_2	CDS	6565397	6566916	6565397	ID=Msa0234080-mRNA-1:cds;Parent=Msa0234080-mRNA-1
chr2_2	three_prime_UTR	6565245	6565396	6565245	ID=Msa0234080-mRNA-1:three_prime_utr;Parent=Msa0234080-mRNA-1

Gene Sequence

>Msa0234080

ATGGATTTGATATTAACTTCATCTGAGAGTGGACAATTGACGGTTGAAAATTCAGTTATTGATGCGGATAGTTCTCAATGTTACTCATTTAGTGATAAAGAATCATGGAAGTCTTTGGTCTTTAAATCTATAAAAGAGATTGAAGATTTTTATTTTAATTATGCATATAACACTGGATTCTCTGTTAGGAGTATGTTGAAGTCCAAGAACAATTCACAAAATAAGAAATCGGATAAGGTACATTATGTGCGTTATGTTTGTAACAAAGAAGGTTTCAAGAAGGGAAGTCTGCTTAATCCTAAAAACAGGCCAACATGTGACAGTCCAGTTCTTGTTGAGTATGAGAAAGCTAAAGAAAAACCAGAGGAAAGGGTTGGTTGTAAAGCTGGAATATATTTGAAGTTGGATGAAGCGTTGAATGTCTATAAGATATATAAGTGGGAAGTTGATCACTGTCATCCATTACATAAACCAGAGCATTTGTGCTACTTGAGATCATTTCGAGAAGTAAATGAAGTTCAAGGACAACTTGCTTTAATAAATTCTAAAGCTGGAATGTCGATGAGAACATCTTATGAAGTGATGGGTCAAGGAGTTGGAGGCACGGAGAATCTTCCTTTTCGATTTTCGGACTTAAAGAACTATCTAATGACAACTCGTCAAAAGGAGATGGTGGCTGGTGAAGCAACTGTTATTCAAGAATTCTTTAGAAATGAAGCTCTCTCGAAACCAGGTTTTTACTATGATATCCAAGTTGATGCTGCAGAAGACATAGCCAGTATTTTTTGGGCAGATGGAATTATGCAACAAGATTATGCTCTATTTGGTGATGTTGTCAGTTTTGATACAACTTACCGAACAAACAATAAATATCGGCCATTAGCTGCCTTTCTTGGTTTTGACAATCATCGTAAGAGTGTGTTATTTGGTGCTGCGCTACTGTATGATGAGACATCAGCTACTTTTGATTGGTTGTTTACAACTTTTTTGAAGTGTATGTCCAATAAGAAACCTCAAACCATATACACAGACCAGGCTGGTGCGTTGTTGAAGTCAATTCCGAATGTTTTTCAAGGTGTTTTTCATGGTCTTTGCTCATGGCACATGGGAGAGAATGCAAAGAAAAATCTCGGCTCTCGTGCAAACAGTGCATTTTTCGATGAGTTAACTAATTTGGTTTCAAATGTCGAAGATGAATCAGATTTCGACTACAATTGGGATCAGATGATGAAAAACTGTTTTAATGGAAGGCCTACTTCGGACTTTAAGTGGCTTGTTCAAACTCATTCAAATCGTATGCATTGGTCTTCAGTTTGGGTGAGGTCACATTTTACAGCTGGTTTGAAAACGACACAGTTAAGTGAGTCTTTCAATGCTTTTCTTCGTGGATTTTTGCAGCCAGACCATTCACTTGTTCGATTCTTTAGTCATTTCAACATTATGGTTCAGAGAATGAGGGATAATCATGCTGAGTTGGACTTTAAGGCTGCAAACACTAGAACAAAAAATAATTATCCCAACAGTCAGCTTATGCGGTCCGTAGTAAACAAGTATACACCATCTTGCTTTTCATTTATTCACAGTCAGTATGACCTTTCCTTCAAATACTATTATGAGGAGGACACAACAAAAGGGTCAGAATTGAACAAGTTCTTCAAAGTTTTCACAATTGAGAAGGTTGATTATAATTACGATGATGTTGATAATGATAATGAGAGGGTTTCGAATGTTGATGTTTTGGATGCAGAATTTGAAGAAAACCTTTCCCCAAGGTTTGAAGATCATAATCGACTTGATGAAAGGGTTGTCACAGTCGACATTGGGAGCAAAAGTTTTACTTGTTCATGTCGTATGTTTGAGAACCGGGGTTTCTTATGTCGACATGTTTTCAAGATTTTGGAGGTCTTAGGTGGTCATGTGCAATATCATTTTTTGAAGACTATACCTGCACAATATGTTTTAAAGCGGTGGACTAGAGATGTGCGTCCGTCTGTCGATAAGCTGAAATCCACCATGAATGTTGGCACTAAAGACACCACTGAAGCGCAACGATATCAACAAATTTGTGCTGTTACAGTTCAGCTTTCTACACGTGTTTGCGCAGATGCGGAGGCATCTGAAATATTTCTCAACGGTGTTCTTGAGGCAGGGAAAAAGGCAGAGGAGTTTCTTATTTCTAAAGGTATTCATACAGATCGATCATCCGTTACGCCTTCTAAGTCTTCAAAAGCAGTTGCTGTCTGTGAACCATCTGTTGGGACTGCACCAAAGTTCAAAGAAAGACCAAATCCAATTAGGTCAAAGAAGCGACTTAAAAGTGATTATGAAAAAGCTAGAGCAAGACAGAAATTCCTTATAGCTCGGAAGAAGCAAAAGAAAGATGATGAGGCTTTAACAAATTAA

Protein sequence

>Msa0234080

MDLILTSSESGQLTVENSVIDADSSQCYSFSDKESWKSLVFKSIKEIEDFYFNYAYNTGFSVRSMLKSKNNSQNKKSDKVHYVRYVCNKEGFKKGSLLNPKNRPTCDSPVLVEYEKAKEKPEERVGCKAGIYLKLDEALNVYKIYKWEVDHCHPLHKPEHLCYLRSFREVNEVQGQLALINSKAGMSMRTSYEVMGQGVGGTENLPFRFSDLKNYLMTTRQKEMVAGEATVIQEFFRNEALSKPGFYYDIQVDAAEDIASIFWADGIMQQDYALFGDVVSFDTTYRTNNKYRPLAAFLGFDNHRKSVLFGAALLYDETSATFDWLFTTFLKCMSNKKPQTIYTDQAGALLKSIPNVFQGVFHGLCSWHMGENAKKNLGSRANSAFFDELTNLVSNVEDESDFDYNWDQMMKNCFNGRPTSDFKWLVQTHSNRMHWSSVWVRSHFTAGLKTTQLSESFNAFLRGFLQPDHSLVRFFSHFNIMVQRMRDNHAELDFKAANTRTKNNYPNSQLMRSVVNKYTPSCFSFIHSQYDLSFKYYYEEDTTKGSELNKFFKVFTIEKVDYNYDDVDNDNERVSNVDVLDAEFEENLSPRFEDHNRLDERVVTVDIGSKSFTCSCRMFENRGFLCRHVFKILEVLGGHVQYHFLKTIPAQYVLKRWTRDVRPSVDKLKSTMNVGTKDTTEAQRYQQICAVTVQLSTRVCADAEASEIFLNGVLEAGKKAEEFLISKGIHTDRSSVTPSKSSKAVAVCEPSVGTAPKFKERPNPIRSKKRLKSDYEKARARQKFLIARKKQKKDDEALTN*