AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0235270


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0235270 MtrunA17_Chr2g0283811 98.137 161 2 1 1 160 1 161 3.29e-116 325
Msa0235270 MtrunA17_Chr6g0453321 70.370 81 23 1 19 99 110 189 1.58e-36 131
Msa0235270 MtrunA17_Chr4g0042481 67.500 80 26 0 20 99 96 175 1.56e-35 128
Msa0235270 MtrunA17_Chr7g0217581 71.053 76 21 1 24 99 211 285 1.24e-34 127
Msa0235270 MtrunA17_Chr8g0355261 67.105 76 24 1 25 99 96 171 4.25e-34 124
Msa0235270 MtrunA17_Chr7g0254161 71.233 73 20 1 28 99 82 154 6.23e-33 120
Msa0235270 MtrunA17_Chr6g0460731 73.239 71 18 1 30 99 79 149 6.45e-32 117
Msa0235270 MtrunA17_Chr5g0417551 60.714 84 31 1 18 99 97 180 2.71e-31 115
Msa0235270 MtrunA17_Chr3g0092111 57.778 90 29 1 19 99 100 189 5.22e-31 115
Msa0235270 MtrunA17_Chr5g0417601 70.423 71 18 1 32 99 123 193 1.59e-30 113
Msa0235270 MtrunA17_Chr6g0460721 69.014 71 21 1 30 99 72 142 1.70e-30 112
Msa0235270 MtrunA17_Chr5g0417571 71.667 60 17 0 40 99 132 191 7.23e-30 110
Msa0235270 MtrunA17_Chr5g0417561 67.647 68 22 0 32 99 105 172 8.17e-30 111
Msa0235270 MtrunA17_Chr2g0328021 55.263 76 34 0 24 99 40 115 2.61e-27 103
Msa0235270 MtrunA17_Chr8g0367161 47.000 100 51 1 24 123 12 109 2.75e-26 101
Msa0235270 MtrunA17_Chr8g0341671 59.155 71 23 1 34 98 2 72 3.98e-26 98.6
Msa0235270 MtrunA17_Chr8g0374251 48.515 101 39 3 48 138 70 167 8.32e-26 100
Msa0235270 MtrunA17_Chr4g0010151 70.175 57 17 0 43 99 79 135 1.36e-25 100
Msa0235270 MtrunA17_Chr8g0374111 71.154 52 15 0 46 97 29 80 4.17e-25 96.3
Msa0235270 MtrunA17_Chr8g0346551 63.636 66 22 1 36 99 12 77 5.55e-25 97.1
Msa0235270 MtrunA17_Chr4g0031411 64.286 70 24 1 43 111 72 141 8.86e-25 97.4
Msa0235270 MtrunA17_Chr2g0326001 55.263 76 34 0 24 99 18 93 1.20e-24 96.7
Msa0235270 MtrunA17_Chr3g0127201 64.912 57 20 0 43 99 24 80 1.42e-24 96.3
Msa0235270 MtrunA17_Chr4g0061761 69.811 53 16 0 44 96 38 90 1.75e-24 96.7
Msa0235270 MtrunA17_Chr2g0308011 73.469 49 13 0 46 94 37 85 2.68e-24 94.0
Msa0235270 MtrunA17_Chr4g0047001 56.098 82 27 2 48 120 57 138 3.36e-24 96.7
Msa0235270 MtrunA17_Chr7g0223311 76.000 50 12 0 48 97 79 128 4.09e-24 96.3
Msa0235270 MtrunA17_Chr7g0237921 68.421 57 18 0 43 99 73 129 8.76e-24 95.1
Msa0235270 MtrunA17_Chr1g0188851 54.412 68 31 0 30 97 27 94 1.25e-23 93.6
Msa0235270 MtrunA17_Chr5g0411951 68.519 54 17 0 43 96 40 93 1.84e-23 94.0
Msa0235270 MtrunA17_Chr4g0047761 67.308 52 17 0 48 99 74 125 8.24e-23 92.0
Msa0235270 MtrunA17_Chr4g0030051 54.054 74 34 0 24 97 19 92 8.86e-23 91.3
Msa0235270 MtrunA17_Chr2g0282441 53.333 75 28 1 30 97 2 76 1.01e-22 92.0
Msa0235270 MtrunA17_Chr1g0177251 64.516 62 21 1 39 99 4 65 1.11e-22 91.3
Msa0235270 MtrunA17_Chr3g0126201 65.517 58 20 0 42 99 47 104 1.23e-22 91.7
Msa0235270 MtrunA17_Chr2g0282511 71.429 49 14 0 46 94 45 93 1.52e-22 91.3
Msa0235270 MtrunA17_Chr2g0282051 41.489 94 50 1 6 99 11 99 1.99e-22 90.1
Msa0235270 MtrunA17_Chr3g0117601 66.667 51 17 0 46 96 61 111 1.23e-21 89.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0235270 AT1G29160.1 62.609 115 23 1 8 102 4 118 7.86e-47 150
Msa0235270 AT2G34140.1 63.964 111 24 1 8 102 4 114 1.43e-45 147
Msa0235270 AT5G62430.1 58.416 101 28 2 12 99 8 107 2.26e-35 125
Msa0235270 AT3G47500.1 66.250 80 27 0 20 99 84 163 5.84e-35 126
Msa0235270 AT5G39660.3 82.540 63 11 0 40 102 132 194 1.97e-34 125
Msa0235270 AT5G39660.1 82.540 63 11 0 40 102 132 194 1.97e-34 125
Msa0235270 AT5G39660.2 82.540 63 11 0 40 102 132 194 1.97e-34 125
Msa0235270 AT1G26790.1 56.311 103 38 1 23 125 88 183 4.57e-34 123
Msa0235270 AT1G69570.1 60.440 91 29 1 34 124 120 203 6.79e-33 120
Msa0235270 AT5G60850.1 57.692 78 33 0 46 123 51 128 8.75e-27 102
Msa0235270 AT1G51700.1 71.698 53 15 0 46 98 33 85 4.58e-26 98.2
Msa0235270 AT3G21270.1 76.923 52 12 0 46 97 29 80 1.07e-25 97.4
Msa0235270 AT2G28810.2 72.727 55 15 0 43 97 74 128 2.23e-25 99.4
Msa0235270 AT3G50410.1 71.698 53 15 0 46 98 30 82 3.07e-25 97.4
Msa0235270 AT2G28810.1 72.727 55 15 0 43 97 91 145 3.67e-25 99.0
Msa0235270 AT2G37590.1 68.421 57 18 0 43 99 86 142 9.04e-25 97.8
Msa0235270 AT3G55370.1 68.421 57 18 0 43 99 73 129 9.19e-25 97.8
Msa0235270 AT3G55370.2 68.421 57 18 0 43 99 73 129 1.01e-24 98.2
Msa0235270 AT3G55370.3 68.421 57 18 0 43 99 118 174 1.39e-24 97.8
Msa0235270 AT5G65590.1 68.421 57 18 0 43 99 38 94 1.52e-24 97.1
Msa0235270 AT1G07640.2 52.500 80 38 0 18 97 49 128 3.21e-24 96.3
Msa0235270 AT1G07640.1 76.000 50 12 0 48 97 23 72 3.68e-24 95.1
Msa0235270 AT1G07640.3 52.500 80 38 0 18 97 57 136 3.83e-24 96.3
Msa0235270 AT4G38000.1 62.712 59 21 1 43 100 38 96 9.20e-24 93.6
Msa0235270 AT5G66940.1 67.925 53 17 0 46 98 32 84 9.55e-24 93.2
Msa0235270 AT5G60200.1 66.667 57 19 0 43 99 52 108 1.10e-23 93.6
Msa0235270 AT5G02460.1 68.421 57 18 0 43 99 92 148 1.83e-23 95.1
Msa0235270 AT3G45610.1 52.778 72 34 0 28 99 22 93 2.12e-23 92.4
Msa0235270 AT4G24060.1 63.158 57 21 0 43 99 50 106 2.51e-23 94.0
Msa0235270 AT1G47655.1 64.815 54 19 0 41 94 24 77 3.22e-23 91.3
Msa0235270 AT1G28310.1 68.519 54 17 0 46 99 27 80 4.69e-23 92.8
Msa0235270 AT3G61850.2 57.812 64 27 0 36 99 52 115 4.71e-23 92.4
Msa0235270 AT3G61850.3 57.812 64 27 0 36 99 52 115 4.71e-23 92.4
Msa0235270 AT3G52440.1 66.102 59 19 1 44 101 22 80 4.72e-23 91.7
Msa0235270 AT3G52440.2 66.102 59 19 1 44 101 41 99 4.89e-23 92.0
Msa0235270 AT3G61850.1 57.812 64 27 0 36 99 64 127 5.55e-23 92.4
Msa0235270 AT1G21340.1 47.872 94 40 2 48 138 40 127 6.57e-23 91.7
Msa0235270 AT1G28310.2 68.519 54 17 0 46 99 51 104 6.88e-23 92.8
Msa0235270 AT3G61850.5 57.812 64 27 0 36 99 52 115 7.42e-23 92.4
Msa0235270 AT3G61850.4 57.812 64 27 0 36 99 64 127 8.01e-23 92.4
Msa0235270 AT1G64620.1 65.385 52 18 0 43 94 46 97 1.13e-22 92.4
Msa0235270 AT2G28510.1 71.154 52 15 0 48 99 50 101 1.19e-22 91.3
Msa0235270 AT4G00940.1 71.154 52 15 0 48 99 70 121 1.75e-22 91.3
Msa0235270 AT4G00940.3 71.154 52 15 0 48 99 70 121 1.75e-22 91.3
Msa0235270 AT4G00940.2 71.154 52 15 0 48 99 70 121 1.75e-22 91.3
Msa0235270 AT5G62940.1 69.388 49 15 0 48 96 75 123 1.33e-21 89.7
Msa0235270 AT4G21050.1 67.308 52 17 0 48 99 26 77 2.36e-21 86.3
Msa0235270 AT2G46590.1 68.750 48 15 0 46 93 68 115 6.47e-21 87.8
Msa0235270 AT2G46590.2 68.750 48 15 0 46 93 80 127 6.81e-21 87.8
Msa0235270 AT4G21040.1 63.462 52 19 0 48 99 27 78 3.48e-19 81.3
Msa0235270 AT4G21080.1 61.538 52 20 0 48 99 27 78 2.52e-18 79.3
Msa0235270 AT4G21030.1 45.588 68 37 0 48 115 23 90 1.65e-13 65.5

Find 45 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATACAAGGGATTAAGCTATT+TGG 0.296201 2_2:-8094704 Msa0235270:CDS
GGTCTTCCTCCGCCGCTTGC+CGG 0.325640 2_2:+8094304 None:intergenic
GCTTCATAGACACAACTTTC+CGG 0.327262 2_2:+8094407 None:intergenic
GTAAGAGCTGTCAAAGATAC+TGG 0.350596 2_2:-8094514 Msa0235270:CDS
ATGTCCAAGATGCAAGAGTA+TGG 0.357770 2_2:-8094591 Msa0235270:CDS
GATGATGGCCATAACTACTT+AGG 0.390058 2_2:-8094374 Msa0235270:CDS
CGGGGCCCTCCGTAACGTTC+CGG 0.411302 2_2:-8094483 Msa0235270:CDS
ATCCTCGTGACCGGGCGGCT+TGG 0.424026 2_2:+8094439 None:intergenic
GTTTCCATACTCTTGCATCT+TGG 0.436933 2_2:+8094587 None:intergenic
AAAGATACTGGACGGCTGGC+GGG 0.444950 2_2:-8094502 Msa0235270:CDS
GCTCTAACCCTAAGTAGTTA+TGG 0.448424 2_2:+8094366 None:intergenic
CGTAACGTTCCGGTTGGTGC+TGG 0.452777 2_2:-8094473 Msa0235270:CDS
TTCCGCCGACCAGCACCAAC+CGG 0.465056 2_2:+8094464 None:intergenic
ATGATGGCCATAACTACTTA+GGG 0.502994 2_2:-8094373 Msa0235270:CDS
TGAACCGCTATCCTCGTGAC+CGG 0.509343 2_2:+8094430 None:intergenic
CAAAGATACTGGACGGCTGG+CGG 0.511046 2_2:-8094503 Msa0235270:CDS
TTCCGGTTGGTGCTGGTCGG+CGG 0.523760 2_2:-8094466 Msa0235270:CDS
AAAAGAAAGTGAAGATCAAA+CGG 0.525439 2_2:-8094642 Msa0235270:CDS
CAGCACCAACCGGAACGTTA+CGG 0.531259 2_2:+8094474 None:intergenic
TTGCATGAGGAATTAAAAGA+AGG 0.537463 2_2:-8094668 Msa0235270:CDS
TGTCAAAGATACTGGACGGC+TGG 0.537711 2_2:-8094506 Msa0235270:CDS
AGAAAAGGTGAAGAGATACA+AGG 0.544735 2_2:-8094719 Msa0235270:CDS
ATACTCTTGCATCTTGGACA+TGG 0.546078 2_2:+8094593 None:intergenic
TCACGAGGATAGCGGTTCAC+CGG 0.553799 2_2:-8094426 Msa0235270:CDS
AGCCAAGCCGCCCGGTCACG+AGG 0.555102 2_2:-8094441 Msa0235270:CDS
CGGCGGAAAGCCAAGCCGCC+CGG 0.555195 2_2:-8094449 Msa0235270:CDS
GCCCTCCGTAACGTTCCGGT+TGG 0.558409 2_2:-8094479 Msa0235270:CDS
ACGTTCCGGTTGGTGCTGGT+CGG 0.560179 2_2:-8094469 Msa0235270:CDS
GAACCGCTATCCTCGTGACC+GGG 0.561130 2_2:+8094431 None:intergenic
AAGCGGCGGAGGAAGACCTC+AGG 0.572311 2_2:-8094299 Msa0235270:CDS
AAGATACTGGACGGCTGGCG+GGG 0.577667 2_2:-8094501 Msa0235270:CDS
AATATGGCTGAAGTGAGAAA+AGG 0.587615 2_2:-8094734 Msa0235270:CDS
GAAAAGGTGAAGAGATACAA+GGG 0.603099 2_2:-8094718 Msa0235270:CDS
CACAACAATAAAATTGCATG+AGG 0.620119 2_2:-8094681 Msa0235270:CDS
GAAGCTGCTAGTTCTGATGA+TGG 0.628789 2_2:-8094389 Msa0235270:CDS
CGGCGGAGGAAGACCTCAGG+TGG 0.630380 2_2:-8094296 Msa0235270:CDS
TGCTAATGAATAACCACCTG+AGG 0.631649 2_2:+8094283 None:intergenic
ACCAACCGGAACGTTACGGA+GGG 0.632472 2_2:+8094478 None:intergenic
GAGCTGTCAAAGATACTGGA+CGG 0.644495 2_2:-8094510 Msa0235270:CDS
CACCAACCGGAACGTTACGG+AGG 0.647589 2_2:+8094477 None:intergenic
TCCCGAAAATCACTCTGAGG+AGG 0.648141 2_2:+8094335 None:intergenic
CCGCCCGGTCACGAGGATAG+CGG 0.672452 2_2:-8094434 Msa0235270:CDS
ACTTCCCGAAAATCACTCTG+AGG 0.686816 2_2:+8094332 None:intergenic
AGAAAGTGAAGATCAAACGG+TGG 0.696077 2_2:-8094639 Msa0235270:CDS
CCGCTATCCTCGTGACCGGG+CGG 0.737412 2_2:+8094434 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAAGAAAGTGAAGATCAAA+CGG - chr2_2:8094360-8094379 Msa0235270:CDS 25.0%
ATACAAGGGATTAAGCTATT+TGG - chr2_2:8094298-8094317 Msa0235270:CDS 30.0%
CACAACAATAAAATTGCATG+AGG - chr2_2:8094321-8094340 Msa0235270:CDS 30.0%
TTGCATGAGGAATTAAAAGA+AGG - chr2_2:8094334-8094353 Msa0235270:CDS 30.0%
AGAAAAGGTGAAGAGATACA+AGG - chr2_2:8094283-8094302 Msa0235270:CDS 35.0%
GAAAAGGTGAAGAGATACAA+GGG - chr2_2:8094284-8094303 Msa0235270:CDS 35.0%
CAGCTCTTACAAAAATGTCT+TGG + chr2_2:8094478-8094497 None:intergenic 35.0%
ATGATGGCCATAACTACTTA+GGG - chr2_2:8094629-8094648 Msa0235270:CDS 35.0%
!!! GATTTTCGGGAAGTATTTTC+CGG - chr2_2:8094679-8094698 Msa0235270:CDS 35.0%
AGAAAGTGAAGATCAAACGG+TGG - chr2_2:8094363-8094382 Msa0235270:CDS 40.0%
ATACTCTTGCATCTTGGACA+TGG + chr2_2:8094412-8094431 None:intergenic 40.0%
ATGTCCAAGATGCAAGAGTA+TGG - chr2_2:8094411-8094430 Msa0235270:CDS 40.0%
GTTTCCATACTCTTGCATCT+TGG + chr2_2:8094418-8094437 None:intergenic 40.0%
GTAAGAGCTGTCAAAGATAC+TGG - chr2_2:8094488-8094507 Msa0235270:CDS 40.0%
GCTTCATAGACACAACTTTC+CGG + chr2_2:8094598-8094617 None:intergenic 40.0%
! GATGATGGCCATAACTACTT+AGG - chr2_2:8094628-8094647 Msa0235270:CDS 40.0%
GCTCTAACCCTAAGTAGTTA+TGG + chr2_2:8094639-8094658 None:intergenic 40.0%
TGCTAATGAATAACCACCTG+AGG + chr2_2:8094722-8094741 None:intergenic 40.0%
GAGCTGTCAAAGATACTGGA+CGG - chr2_2:8094492-8094511 Msa0235270:CDS 45.0%
!! GAAGCTGCTAGTTCTGATGA+TGG - chr2_2:8094613-8094632 Msa0235270:CDS 45.0%
! TGCCTCCTCAGAGTGATTTT+CGG - chr2_2:8094665-8094684 Msa0235270:CDS 45.0%
ACTTCCCGAAAATCACTCTG+AGG + chr2_2:8094673-8094692 None:intergenic 45.0%
TGTCAAAGATACTGGACGGC+TGG - chr2_2:8094496-8094515 Msa0235270:CDS 50.0%
! GCCTCCTCAGAGTGATTTTC+GGG - chr2_2:8094666-8094685 Msa0235270:CDS 50.0%
! TCCCGAAAATCACTCTGAGG+AGG + chr2_2:8094670-8094689 None:intergenic 50.0%
!!! GGGAAGTATTTTCCGGCAAG+CGG - chr2_2:8094686-8094705 Msa0235270:CDS 50.0%
! AAGTATTTTCCGGCAAGCGG+CGG - chr2_2:8094689-8094708 Msa0235270:CDS 50.0%
CAAAGATACTGGACGGCTGG+CGG - chr2_2:8094499-8094518 Msa0235270:CDS 55.0%
AAAGATACTGGACGGCTGGC+GGG - chr2_2:8094500-8094519 Msa0235270:CDS 55.0%
ACCAACCGGAACGTTACGGA+GGG + chr2_2:8094527-8094546 None:intergenic 55.0%
CAGCACCAACCGGAACGTTA+CGG + chr2_2:8094531-8094550 None:intergenic 55.0%
TGAACCGCTATCCTCGTGAC+CGG + chr2_2:8094575-8094594 None:intergenic 55.0%
! TCACGAGGATAGCGGTTCAC+CGG - chr2_2:8094576-8094595 Msa0235270:CDS 55.0%
AAGATACTGGACGGCTGGCG+GGG - chr2_2:8094501-8094520 Msa0235270:CDS 60.0%
CACCAACCGGAACGTTACGG+AGG + chr2_2:8094528-8094547 None:intergenic 60.0%
! CGTAACGTTCCGGTTGGTGC+TGG - chr2_2:8094529-8094548 Msa0235270:CDS 60.0%
!! ACGTTCCGGTTGGTGCTGGT+CGG - chr2_2:8094533-8094552 Msa0235270:CDS 60.0%
GAACCGCTATCCTCGTGACC+GGG + chr2_2:8094574-8094593 None:intergenic 60.0%
! TATTTTCCGGCAAGCGGCGG+AGG - chr2_2:8094692-8094711 Msa0235270:CDS 60.0%
GCCCTCCGTAACGTTCCGGT+TGG - chr2_2:8094523-8094542 Msa0235270:CDS 65.0%
!! TTCCGGTTGGTGCTGGTCGG+CGG - chr2_2:8094536-8094555 Msa0235270:CDS 65.0%
TTCCGCCGACCAGCACCAAC+CGG + chr2_2:8094541-8094560 None:intergenic 65.0%
AAGCGGCGGAGGAAGACCTC+AGG - chr2_2:8094703-8094722 Msa0235270:CDS 65.0%
CGGGGCCCTCCGTAACGTTC+CGG - chr2_2:8094519-8094538 Msa0235270:CDS 70.0%
ATCCTCGTGACCGGGCGGCT+TGG + chr2_2:8094566-8094585 None:intergenic 70.0%
CCGCTATCCTCGTGACCGGG+CGG + chr2_2:8094571-8094590 None:intergenic 70.0%
CCGCCCGGTCACGAGGATAG+CGG - chr2_2:8094568-8094587 Msa0235270:CDS 70.0%
GGTCTTCCTCCGCCGCTTGC+CGG + chr2_2:8094701-8094720 None:intergenic 70.0%
CGGCGGAGGAAGACCTCAGG+TGG - chr2_2:8094706-8094725 Msa0235270:CDS 70.0%
CGGCGGAAAGCCAAGCCGCC+CGG - chr2_2:8094553-8094572 Msa0235270:CDS 75.0%
AGCCAAGCCGCCCGGTCACG+AGG - chr2_2:8094561-8094580 Msa0235270:CDS 75.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 8094271 8094753 8094271 ID=Msa0235270;Name=Msa0235270
chr2_2 mRNA 8094271 8094753 8094271 ID=Msa0235270-mRNA-1;Parent=Msa0235270;Name=Msa0235270-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|160
chr2_2 exon 8094271 8094753 8094271 ID=Msa0235270-mRNA-1:exon:3525;Parent=Msa0235270-mRNA-1
chr2_2 CDS 8094271 8094753 8094271 ID=Msa0235270-mRNA-1:cds;Parent=Msa0235270-mRNA-1
Gene Sequence

>Msa0235270

ATGGCTGAAGTGAGAAAAGGTGAAGAGATACAAGGGATTAAGCTATTTGGCACAACAATAAAATTGCATGAGGAATTAAAAGAAGGTGAAAAAGAAAGTGAAGATCAAACGGTGGAGAAGAAGATAGAAAAGATCATACCATGTCCAAGATGCAAGAGTATGGAAACAAAATTTTGTTATTTCAATAATTACAACGTTAATCAACCAAGACATTTTTGTAAGAGCTGTCAAAGATACTGGACGGCTGGCGGGGCCCTCCGTAACGTTCCGGTTGGTGCTGGTCGGCGGAAAGCCAAGCCGCCCGGTCACGAGGATAGCGGTTCACCGGAAAGTTGTGTCTATGAAGCTGCTAGTTCTGATGATGGCCATAACTACTTAGGGTTAGAGCAATTTATGCCTCCTCAGAGTGATTTTCGGGAAGTATTTTCCGGCAAGCGGCGGAGGAAGACCTCAGGTGGTTATTCATTAGCATCCTCCTTGTGA

Protein sequence

>Msa0235270

MAEVRKGEEIQGIKLFGTTIKLHEELKEGEKESEDQTVEKKIEKIIPCPRCKSMETKFCYFNNYNVNQPRHFCKSCQRYWTAGGALRNVPVGAGRRKAKPPGHEDSGSPESCVYEAASSDDGHNYLGLEQFMPPQSDFREVFSGKRRRKTSGGYSLASSL*