AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0239270

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0239270 MtrunA17_Chr2g0292521 77.778 135 30 0 45 179 883 1017 4.75e-68 223
Msa0239270 MtrunA17_Chr2g0292521 88.235 34 4 0 3 36 742 775 3.04e-12 64.3
Msa0239270 MtrunA17_Chr2g0316131 59.524 126 34 1 45 170 16 124 7.91e-45 144
Msa0239270 MtrunA17_Chr2g0316171 59.524 126 34 1 45 170 16 124 7.91e-45 144
Msa0239270 MtrunA17_Chr3g0129081 53.030 132 61 1 45 176 851 981 4.96e-41 147
Msa0239270 MtrunA17_Chr5g0440581 44.444 135 75 0 45 179 809 943 7.44e-35 129
Msa0239270 MtrunA17_Chr5g0437741 41.270 126 67 3 49 174 595 713 1.65e-20 88.2
Msa0239270 MtrunA17_Chr4g0062911 31.783 129 80 3 46 174 445 565 1.93e-15 73.6
Msa0239270 MtrunA17_Chr2g0334191 35.659 129 75 5 49 174 892 1015 1.41e-14 70.9
Msa0239270 MtrunA17_Chr4g0058561 36.719 128 71 5 46 169 451 572 1.56e-14 70.9
Msa0239270 MtrunA17_Chr4g0046511 36.879 141 79 5 32 168 779 913 1.58e-14 70.9
Msa0239270 MtrunA17_Chr3g0134221 36.066 122 67 6 50 169 492 604 2.57e-14 70.1
Msa0239270 MtrunA17_Chr1g0213501 31.788 151 96 5 29 176 198 344 4.16e-14 69.3
Msa0239270 MtrunA17_Chr5g0414561 35.821 134 80 4 45 176 441 570 4.50e-14 69.3
Msa0239270 MtrunA17_Chr7g0239381 35.821 134 80 4 45 176 438 567 8.64e-14 68.6
Msa0239270 MtrunA17_Chr4g0053221 40.833 120 63 5 50 169 464 575 8.80e-14 68.6
Msa0239270 MtrunA17_Chr5g0437731 43.750 80 42 2 70 149 48 124 3.98e-13 63.2
Msa0239270 MtrunA17_Chr5g0407721 34.286 140 82 5 41 176 457 590 5.42e-13 66.2
Msa0239270 MtrunA17_Chr4g0035464 34.752 141 81 6 32 168 767 900 6.11e-13 66.2
Msa0239270 MtrunA17_Chr8g0393211 34.109 129 75 5 52 179 479 598 6.68e-13 66.2
Msa0239270 MtrunA17_Chr2g0333691 37.931 116 63 5 37 149 209 318 7.60e-13 65.9
Msa0239270 MtrunA17_Chr4g0005201 34.127 126 76 3 49 174 603 721 9.02e-13 65.9
Msa0239270 MtrunA17_Chr1g0160711 30.469 128 85 3 52 176 430 556 9.72e-13 65.5
Msa0239270 MtrunA17_Chr8g0366981 31.298 131 85 3 40 168 391 518 1.97e-12 64.7
Msa0239270 MtrunA17_Chr8g0380951 31.783 129 76 5 48 169 780 903 3.16e-12 64.3
Msa0239270 MtrunA17_Chr8g0373501 35.772 123 72 4 49 169 435 552 3.58e-12 63.9
Msa0239270 MtrunA17_Chr1g0183231 31.746 126 78 5 49 172 806 925 3.65e-12 63.9
Msa0239270 MtrunA17_Chr8g0363191 33.333 126 77 4 45 168 515 635 3.77e-12 63.9
Msa0239270 MtrunA17_Chr6g0487011 31.496 127 78 5 29 149 17 140 3.84e-12 62.0
Msa0239270 MtrunA17_Chr2g0277081 33.557 149 87 7 32 176 770 910 3.98e-12 63.9
Msa0239270 MtrunA17_Chr4g0054521 33.803 142 83 6 32 169 772 906 4.22e-12 63.9
Msa0239270 MtrunA17_Chr6g0464171 35.433 127 72 5 34 153 809 932 4.61e-12 63.5
Msa0239270 MtrunA17_Chr1g0211611 35.135 111 66 5 46 156 396 500 4.63e-12 63.5
Msa0239270 MtrunA17_Chr8g0380941 31.538 130 75 6 48 169 811 934 5.57e-12 63.5
Msa0239270 MtrunA17_Chr1g0194101 31.788 151 96 5 29 176 153 299 5.70e-12 63.2
Msa0239270 MtrunA17_Chr1g0175731 31.126 151 97 5 29 176 165 311 6.75e-12 62.8
Msa0239270 MtrunA17_Chr1g0190411 30.508 177 98 8 12 169 758 928 7.00e-12 63.2
Msa0239270 MtrunA17_Chr5g0406841 31.206 141 83 7 45 179 252 384 7.02e-12 63.2
Msa0239270 MtrunA17_Chr1g0158511 28.000 150 95 5 23 168 359 499 7.07e-12 63.2
Msa0239270 MtrunA17_Chr7g0258951 31.126 151 97 5 29 176 161 307 7.51e-12 62.8
Msa0239270 MtrunA17_Chr5g0442151 34.559 136 77 8 49 178 781 910 7.58e-12 63.2
Msa0239270 MtrunA17_Chr6g0486961 32.000 125 76 5 29 147 763 884 7.74e-12 63.2
Msa0239270 MtrunA17_Chr4g0069241 33.775 151 93 5 29 176 152 298 7.77e-12 62.8
Msa0239270 MtrunA17_Chr3g0117731 36.207 116 68 4 50 165 161 270 8.11e-12 62.8
Msa0239270 MtrunA17_Chr4g0018701 32.540 126 76 5 49 172 824 942 8.49e-12 62.8
Msa0239270 MtrunA17_Chr1g0158471 27.333 150 96 5 23 168 359 499 8.64e-12 62.8
Msa0239270 MtrunA17_Chr5g0413981 30.827 133 80 5 32 157 147 274 8.96e-12 62.4
Msa0239270 MtrunA17_Chr8g0362931 35.514 107 64 3 45 149 983 1086 1.15e-11 62.8
Msa0239270 MtrunA17_Chr8g0364671 31.298 131 82 4 32 157 144 271 1.19e-11 62.0
Msa0239270 MtrunA17_Chr5g0406251 33.628 113 69 5 50 162 394 500 1.33e-11 62.4
Msa0239270 MtrunA17_Chr7g0270121 34.921 126 71 6 49 169 482 601 1.35e-11 62.4
Msa0239270 MtrunA17_Chr1g0194541 36.296 135 69 8 49 174 393 519 1.44e-11 62.0
Msa0239270 MtrunA17_Chr6g0464211 35.200 125 71 5 36 153 810 931 1.45e-11 62.4
Msa0239270 MtrunA17_Chr4g0049601 27.068 133 90 4 46 176 454 581 1.92e-11 62.0
Msa0239270 MtrunA17_Chr6g0478621 32.558 129 77 5 52 179 482 601 2.00e-11 61.6
Msa0239270 MtrunA17_Chr8g0363501 34.234 111 68 3 45 153 334 441 2.04e-11 61.6
Msa0239270 MtrunA17_Chr1g0157231 33.043 115 67 5 50 162 244 350 2.16e-11 61.6
Msa0239270 MtrunA17_Chr1g0158761 27.517 149 99 4 31 176 420 562 2.34e-11 61.6
Msa0239270 MtrunA17_Chr6g0464141 34.646 127 73 5 34 153 809 932 2.40e-11 61.6
Msa0239270 MtrunA17_Chr1g0209521 33.835 133 75 5 49 174 459 585 2.42e-11 61.6
Msa0239270 MtrunA17_Chr7g0239361 33.058 121 68 4 45 155 453 570 2.48e-11 61.6
Msa0239270 MtrunA17_Chr2g0300201 35.433 127 72 6 34 153 809 932 2.64e-11 61.6
Msa0239270 MtrunA17_Chr5g0434141 35.714 126 75 4 46 169 692 813 2.81e-11 61.6
Msa0239270 MtrunA17_Chr8g0362841 32.540 126 78 4 45 168 640 760 2.92e-11 61.2
Msa0239270 MtrunA17_Chr1g0159161 31.343 134 79 5 46 179 389 509 2.95e-11 61.2
Msa0239270 MtrunA17_Chr8g0362431 34.234 111 68 3 45 153 596 703 3.25e-11 61.2
Msa0239270 MtrunA17_Chr3g0144551 30.827 133 86 4 49 179 234 362 3.47e-11 60.8
Msa0239270 MtrunA17_Chr5g0408571 32.558 129 77 4 34 155 287 412 3.60e-11 60.8
Msa0239270 MtrunA17_Chr5g0439561 35.484 124 69 6 45 164 429 545 4.12e-11 60.8
Msa0239270 MtrunA17_Chr8g0344471 28.058 139 89 4 46 181 495 625 4.14e-11 60.8
Msa0239270 MtrunA17_Chr4g0063691 32.710 107 68 3 45 149 633 737 4.19e-11 60.8
Msa0239270 MtrunA17_Chr3g0143961 31.655 139 80 7 45 176 164 294 4.31e-11 60.5
Msa0239270 MtrunA17_Chr7g0221431 34.965 143 78 6 32 169 221 353 4.55e-11 60.5
Msa0239270 MtrunA17_Chr6g0487031 37.864 103 58 4 48 147 805 904 4.93e-11 60.8
Msa0239270 MtrunA17_Chr8g0363401 34.234 111 68 3 45 153 603 710 5.19e-11 60.5
Msa0239270 MtrunA17_Chr2g0324171 33.846 130 71 6 45 169 440 559 5.35e-11 60.5
Msa0239270 MtrunA17_Chr8g0392271 31.967 122 77 4 49 168 680 797 5.41e-11 60.5
Msa0239270 MtrunA17_Chr1g0160761 28.125 128 88 3 52 176 437 563 7.74e-11 60.1
Msa0239270 MtrunA17_Chr3g0127671 32.857 140 79 6 48 176 829 964 7.74e-11 60.1
Msa0239270 MtrunA17_Chr3g0140861 35.238 105 61 4 49 149 760 861 7.81e-11 60.1
Msa0239270 MtrunA17_Chr6g0464161 34.646 127 73 5 34 153 538 661 7.97e-11 60.1
Msa0239270 MtrunA17_Chr7g0236881 31.538 130 82 5 42 168 708 833 8.02e-11 60.1
Msa0239270 MtrunA17_Chr5g0408821 35.156 128 71 5 36 155 204 327 8.87e-11 59.7
Msa0239270 MtrunA17_Chr1g0181731 31.579 133 80 5 48 175 697 823 9.48e-11 60.1
Msa0239270 MtrunA17_Chr5g0428581 33.333 135 75 6 30 158 128 253 9.59e-11 59.7
Msa0239270 MtrunA17_Chr2g0324471 37.273 110 59 5 45 149 464 568 9.87e-11 59.7
Msa0239270 MtrunA17_Chr7g0248491 30.709 127 82 4 49 173 261 383 9.98e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0239270 AT4G20940.1 66.443 149 49 1 31 179 840 987 3.64e-62 207
Msa0239270 AT5G10020.1 49.686 159 74 3 21 176 842 997 1.10e-44 157
Msa0239270 AT5G10020.2 49.686 159 74 3 21 176 794 949 1.26e-44 157
Msa0239270 AT2G27060.3 42.748 131 75 0 49 179 741 871 1.53e-32 123
Msa0239270 AT2G27060.1 42.748 131 75 0 49 179 834 964 1.55e-32 123
Msa0239270 AT1G15530.1 36.641 131 76 4 48 176 458 583 6.08e-18 80.9
Msa0239270 AT3G51740.1 35.714 126 74 3 49 174 637 755 1.50e-16 77.0
Msa0239270 AT2G26730.1 32.653 147 83 5 35 174 430 567 3.02e-16 76.3
Msa0239270 AT5G01540.1 34.921 126 72 4 45 168 464 581 2.30e-15 73.6
Msa0239270 AT3G50230.2 36.641 131 76 5 50 179 499 623 2.45e-15 73.6
Msa0239270 AT3G50230.1 36.641 131 76 5 50 179 487 611 2.47e-15 73.6
Msa0239270 AT1G70110.1 32.386 176 92 8 9 168 396 560 1.27e-14 71.6
Msa0239270 AT5G01560.1 33.835 133 78 4 45 175 461 585 1.61e-14 71.2
Msa0239270 AT2G43230.1 31.788 151 96 5 29 176 197 343 3.92e-14 69.7
Msa0239270 AT2G43230.2 31.788 151 96 5 29 176 231 377 5.53e-14 69.3
Msa0239270 AT3G59350.6 32.450 151 95 5 29 176 214 360 7.21e-14 68.9
Msa0239270 AT3G59350.2 32.450 151 95 5 29 176 157 303 7.36e-14 68.9
Msa0239270 AT3G59350.3 32.450 151 95 5 29 176 199 345 7.96e-14 68.9
Msa0239270 AT3G59350.1 32.450 151 95 5 29 176 199 345 7.96e-14 68.9
Msa0239270 AT3G59350.4 32.450 151 95 5 29 176 199 345 8.28e-14 68.9
Msa0239270 AT2G30740.5 33.113 151 94 5 29 176 157 303 1.05e-13 68.6
Msa0239270 AT2G30740.2 33.113 151 94 5 29 176 157 303 1.05e-13 68.6
Msa0239270 AT2G30740.10 33.113 151 94 5 29 176 157 303 1.05e-13 68.6
Msa0239270 AT2G30740.11 33.113 151 94 5 29 176 157 303 1.05e-13 68.6
Msa0239270 AT2G30740.6 33.113 151 94 5 29 176 157 303 1.05e-13 68.6
Msa0239270 AT2G30740.15 33.113 151 94 5 29 176 157 303 1.05e-13 68.6
Msa0239270 AT2G30740.4 33.113 151 94 5 29 176 157 303 1.05e-13 68.6
Msa0239270 AT2G30740.14 33.113 151 94 5 29 176 157 303 1.05e-13 68.6
Msa0239270 AT2G30740.7 33.113 151 94 5 29 176 157 303 1.05e-13 68.6
Msa0239270 AT2G30740.1 33.113 151 94 5 29 176 157 303 1.05e-13 68.6
Msa0239270 AT2G30740.13 33.113 151 94 5 29 176 157 303 1.05e-13 68.6
Msa0239270 AT2G30740.8 33.113 151 94 5 29 176 157 303 1.05e-13 68.6
Msa0239270 AT2G30740.12 33.113 151 94 5 29 176 157 303 1.05e-13 68.6
Msa0239270 AT2G30740.3 33.113 151 94 5 29 176 157 303 1.05e-13 68.6
Msa0239270 AT2G30740.9 33.113 151 94 5 29 176 157 303 1.05e-13 68.6
Msa0239270 AT1G68400.1 36.923 130 69 6 49 174 464 584 1.98e-13 68.2
Msa0239270 AT1G06700.1 32.450 151 95 5 29 176 154 300 3.33e-13 67.0
Msa0239270 AT1G06700.3 32.450 151 95 5 29 176 154 300 3.33e-13 67.0
Msa0239270 AT1G06700.2 32.450 151 95 5 29 176 154 300 3.33e-13 67.0
Msa0239270 AT1G24030.4 33.083 133 77 5 32 157 146 273 3.45e-13 66.6
Msa0239270 AT1G48480.1 32.168 143 81 6 35 169 450 584 4.24e-13 67.0
Msa0239270 AT1G24030.2 33.083 133 77 5 32 157 146 273 5.30e-13 66.6
Msa0239270 AT1G24030.6 33.083 133 77 5 32 157 55 182 5.62e-13 65.9
Msa0239270 AT1G24030.3 33.083 133 77 5 32 157 92 219 5.71e-13 66.2
Msa0239270 AT1G24030.1 33.083 133 77 5 32 157 160 287 6.63e-13 66.2
Msa0239270 AT5G43020.1 31.788 151 98 3 27 175 456 603 9.68e-13 65.9
Msa0239270 AT5G25930.1 29.452 146 92 6 37 178 781 919 1.02e-12 65.9
Msa0239270 AT5G65700.1 33.803 142 83 6 32 169 777 911 1.58e-12 65.5
Msa0239270 AT5G65700.2 33.803 142 83 6 32 169 777 911 1.58e-12 65.5
Msa0239270 AT4G29050.2 31.944 144 83 5 32 168 440 575 1.71e-12 65.5
Msa0239270 AT4G29050.1 31.944 144 83 5 32 168 426 561 1.84e-12 65.1
Msa0239270 AT1G24650.1 37.302 126 73 4 46 169 647 768 2.00e-12 65.1
Msa0239270 AT1G61370.2 34.146 123 61 5 48 160 448 560 2.18e-12 65.1
Msa0239270 AT3G49670.1 33.099 142 84 6 32 169 773 907 2.24e-12 65.1
Msa0239270 AT1G61370.1 34.146 123 61 5 48 160 599 711 2.26e-12 65.1
Msa0239270 AT3G55550.1 33.835 133 82 4 45 175 442 570 2.53e-12 64.7
Msa0239270 AT1G70130.1 34.091 132 67 7 46 168 430 550 2.60e-12 64.7
Msa0239270 AT2G15300.1 34.783 138 73 8 45 176 573 699 3.42e-12 64.3
Msa0239270 AT2G48010.1 33.333 132 80 5 49 177 386 512 3.73e-12 64.3
Msa0239270 AT5G67200.1 36.364 121 70 5 50 169 497 611 4.42e-12 63.9
Msa0239270 AT3G24550.1 34.375 128 74 5 46 169 375 496 4.44e-12 63.9
Msa0239270 AT1G08590.1 30.147 136 87 5 44 177 823 952 4.64e-12 64.3
Msa0239270 AT1G66150.1 37.008 127 72 6 46 169 688 809 4.68e-12 63.9
Msa0239270 AT1G11050.1 33.962 159 90 7 32 181 380 532 5.27e-12 63.9
Msa0239270 AT1G31420.1 28.188 149 97 5 32 176 387 529 5.46e-12 63.9
Msa0239270 AT1G31420.3 28.188 149 97 5 32 176 387 529 5.46e-12 63.9
Msa0239270 AT4G28650.1 32.061 131 81 5 49 177 805 929 8.37e-12 63.5
Msa0239270 AT4G28350.1 33.071 127 74 6 45 168 445 563 8.48e-12 63.2
Msa0239270 AT5G16590.1 33.058 121 71 5 49 168 443 554 1.22e-11 62.8
Msa0239270 AT5G55830.1 29.927 137 83 4 46 176 464 593 1.23e-11 62.8
Msa0239270 AT5G61480.1 34.375 128 67 6 51 172 828 944 1.31e-11 62.8
Msa0239270 AT4G34440.2 38.211 123 69 5 49 169 410 527 1.32e-11 62.8
Msa0239270 AT4G34440.1 38.211 123 69 5 49 169 410 527 1.32e-11 62.8
Msa0239270 AT1G29730.4 29.730 148 94 6 28 170 717 859 1.91e-11 62.4
Msa0239270 AT5G60270.1 34.906 106 63 3 46 149 439 540 2.39e-11 62.0
Msa0239270 AT1G29730.3 28.571 147 96 5 28 170 750 891 2.44e-11 62.0
Msa0239270 AT3G53810.1 30.323 155 98 4 32 179 429 580 2.56e-11 62.0
Msa0239270 AT4G20270.1 33.071 127 76 6 49 171 810 931 2.85e-11 62.0
Msa0239270 AT1G29730.1 30.345 145 91 6 28 167 717 856 3.38e-11 61.6
Msa0239270 AT1G29730.2 29.167 144 93 5 28 167 750 888 3.88e-11 61.6
Msa0239270 AT1G75820.1 33.099 142 84 6 32 169 774 908 3.94e-11 61.2
Msa0239270 AT1G69270.1 28.467 137 88 6 51 182 361 492 4.10e-11 61.2
Msa0239270 AT4G02420.1 31.655 139 90 3 32 168 432 567 4.39e-11 61.2
Msa0239270 AT4G32710.1 34.127 126 74 5 49 169 487 608 4.78e-11 61.2
Msa0239270 AT2G31880.1 32.168 143 89 5 32 169 444 583 5.58e-11 60.8
Msa0239270 AT2G30730.1 31.783 129 81 5 49 174 153 277 6.17e-11 60.5
Msa0239270 AT2G30730.2 31.783 129 81 5 49 174 163 287 6.52e-11 60.5
Msa0239270 AT1G31420.2 29.545 132 83 5 49 176 403 528 7.02e-11 60.5
Msa0239270 AT2G01820.1 34.586 133 80 5 46 176 685 812 7.28e-11 60.5
Msa0239270 AT5G63930.1 31.884 138 72 7 41 169 898 1022 8.40e-11 60.5
Msa0239270 AT5G63930.2 31.884 138 72 7 41 169 834 958 8.58e-11 60.5
Msa0239270 AT5G20690.1 26.429 140 92 4 45 181 481 612 9.38e-11 60.1

Find 31 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAACACATTTATAGCTTTA+AGG 0.096850 2_2:+13541919 None:intergenic
TTTGACCAAGTTGTTCTTCA+TGG 0.252272 2_2:-13541945 Msa0239270:CDS
AAGAGCACCAACCGAAGTTT+TGG 0.266027 2_2:-13542676 Msa0239270:CDS
GAAGAACAACTTGGTCAAAA+TGG 0.306780 2_2:+13541949 None:intergenic
ATTGAAAAGATTCTTGATAC+TGG 0.323331 2_2:-13541825 Msa0239270:CDS
ATTCTTGATACTGGTGCCTT+AGG 0.351134 2_2:-13541816 Msa0239270:CDS
CTTCTTTGAAGCAGCTAACT+TGG 0.356377 2_2:+13541782 None:intergenic
CACATCACTATCACACCTTC+TGG 0.366588 2_2:+13541698 None:intergenic
TTCTTTGAAGCAGCTAACTT+GGG 0.433499 2_2:+13541783 None:intergenic
AGGTTGTCGGATAGATTGAT+TGG 0.460697 2_2:-13542736 Msa0239270:CDS
GGTCAAAATGGAGATAGTTC+AGG 0.478130 2_2:+13541961 None:intergenic
TGTGATAGTGATGTGATAAC+TGG 0.492139 2_2:-13541690 Msa0239270:CDS
CTTCTTCCCAAAACTTCGGT+TGG 0.510353 2_2:+13542669 None:intergenic
TACCAGCTTGAGTCATAAGT+TGG 0.523324 2_2:+13541850 None:intergenic
GTCAATGAATCACCAAACTC+GGG 0.547212 2_2:+13542787 None:intergenic
GAGGTGTTGATCTTACAGAT+TGG 0.548922 2_2:-13541659 Msa0239270:CDS
AGTCAATGAATCACCAAACT+CGG 0.552452 2_2:+13542786 None:intergenic
CACCAACTTATGACTCAAGC+TGG 0.557119 2_2:-13541852 Msa0239270:CDS
ACTACTTCTTCCCAAAACTT+CGG 0.563682 2_2:+13542665 None:intergenic
TCTGAGTTGAAGGAAGGCAT+TGG 0.567808 2_2:+13541759 None:intergenic
CTTGGGTGCGTGATAACCTA+AGG 0.568146 2_2:+13541800 None:intergenic
GGTTTCCATGAAGAACAACT+TGG 0.568393 2_2:+13541940 None:intergenic
AAGACTAGATACGAGGTTGT+CGG 0.572239 2_2:-13542749 Msa0239270:CDS
GATGTGATAACTGGTGAGGA+AGG 0.605510 2_2:-13541681 Msa0239270:CDS
AAAACATCTGAGTTGAAGGA+AGG 0.606096 2_2:+13541753 None:intergenic
GTGATAACTGGTGAGGAAGG+AGG 0.620636 2_2:-13541678 Msa0239270:CDS
CTAAGATTGCGAGTAGCAGA+AGG 0.624928 2_2:-13541636 Msa0239270:CDS
CTAGCAGTTGACGTAGCATG+TGG 0.650949 2_2:-13541984 Msa0239270:CDS
TAGTGATGTGATAACTGGTG+AGG 0.708049 2_2:-13541685 Msa0239270:CDS
ATCTTACAAGACTAGATACG+AGG 0.724775 2_2:-13542756 Msa0239270:CDS
TCAATGAATCACCAAACTCG+GGG 0.732009 2_2:+13542788 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGTATAAAAATTAGATATA+AGG + chr2_2:13542051-13542070 None:intergenic 10.0%
!! ATGTATAAAAATTAGATATA+AGG + chr2_2:13542051-13542070 None:intergenic 10.0%
!!! GAAGAATTTTTTAATTAAGC+AGG + chr2_2:13542005-13542024 None:intergenic 20.0%
!!! TTTTTAAACTTTTAACCAGA+AGG - chr2_2:13542691-13542710 Msa0239270:CDS 20.0%
!!! GAAGAATTTTTTAATTAAGC+AGG + chr2_2:13542005-13542024 None:intergenic 20.0%
!!! TTTTTAAACTTTTAACCAGA+AGG - chr2_2:13542691-13542710 Msa0239270:CDS 20.0%
!!! CATTTTCTTGATGATACTTT+AGG - chr2_2:13541698-13541717 Msa0239270:CDS 25.0%
!!! AAGAGTTTGTTAAAGAGATA+AGG - chr2_2:13541855-13541874 Msa0239270:CDS 25.0%
! GAAATAAAACTAGAGTTGTT+TGG - chr2_2:13541915-13541934 Msa0239270:CDS 25.0%
!!! AAAAAATCTAAGGCTTTGTT+TGG + chr2_2:13542136-13542155 None:intergenic 25.0%
!!! CAAAGCCTTAGATTTTTTTT+TGG - chr2_2:13542138-13542157 Msa0239270:intron 25.0%
! GGAAACCAAAAAAAAATCTA+AGG + chr2_2:13542146-13542165 None:intergenic 25.0%
!!! TAGATTTTTTTTTGGTTTCC+AGG - chr2_2:13542146-13542165 Msa0239270:intron 25.0%
!! ACAACACATTTATAGCTTTA+AGG + chr2_2:13542488-13542507 None:intergenic 25.0%
! ATTGAAAAGATTCTTGATAC+TGG - chr2_2:13542579-13542598 Msa0239270:intron 25.0%
!!! CATTTTCTTGATGATACTTT+AGG - chr2_2:13541698-13541717 Msa0239270:CDS 25.0%
!!! AAGAGTTTGTTAAAGAGATA+AGG - chr2_2:13541855-13541874 Msa0239270:CDS 25.0%
! GAAATAAAACTAGAGTTGTT+TGG - chr2_2:13541915-13541934 Msa0239270:CDS 25.0%
!!! AAAAAATCTAAGGCTTTGTT+TGG + chr2_2:13542136-13542155 None:intergenic 25.0%
!!! CAAAGCCTTAGATTTTTTTT+TGG - chr2_2:13542138-13542157 Msa0239270:intron 25.0%
! GGAAACCAAAAAAAAATCTA+AGG + chr2_2:13542146-13542165 None:intergenic 25.0%
!!! TAGATTTTTTTTTGGTTTCC+AGG - chr2_2:13542146-13542165 Msa0239270:intron 25.0%
!! ACAACACATTTATAGCTTTA+AGG + chr2_2:13542488-13542507 None:intergenic 25.0%
! ATTGAAAAGATTCTTGATAC+TGG - chr2_2:13542579-13542598 Msa0239270:intron 25.0%
!! AACTCAGATGTTTTTGCTTT+TGG - chr2_2:13542660-13542679 Msa0239270:intron 30.0%
!! AACTCAGATGTTTTTGCTTT+TGG - chr2_2:13542660-13542679 Msa0239270:intron 30.0%
AGTCAATGAATCACCAAACT+CGG + chr2_2:13541621-13541640 None:intergenic 35.0%
ATCTTACAAGACTAGATACG+AGG - chr2_2:13541648-13541667 Msa0239270:CDS 35.0%
ACTACTTCTTCCCAAAACTT+CGG + chr2_2:13541742-13541761 None:intergenic 35.0%
TACATGGCAACTTTGGATAA+TGG - chr2_2:13541774-13541793 Msa0239270:CDS 35.0%
CTTTAATTCTCTGATCGACT+AGG - chr2_2:13542357-13542376 Msa0239270:intron 35.0%
GAAGAACAACTTGGTCAAAA+TGG + chr2_2:13542458-13542477 None:intergenic 35.0%
TTTGACCAAGTTGTTCTTCA+TGG - chr2_2:13542459-13542478 Msa0239270:intron 35.0%
!! TTCTTTGAAGCAGCTAACTT+GGG + chr2_2:13542624-13542643 None:intergenic 35.0%
! AAAACATCTGAGTTGAAGGA+AGG + chr2_2:13542654-13542673 None:intergenic 35.0%
! AGCAAAAACATCTGAGTTGA+AGG + chr2_2:13542658-13542677 None:intergenic 35.0%
! TGTGATAGTGATGTGATAAC+TGG - chr2_2:13542714-13542733 Msa0239270:CDS 35.0%
AGTCAATGAATCACCAAACT+CGG + chr2_2:13541621-13541640 None:intergenic 35.0%
ATCTTACAAGACTAGATACG+AGG - chr2_2:13541648-13541667 Msa0239270:CDS 35.0%
ACTACTTCTTCCCAAAACTT+CGG + chr2_2:13541742-13541761 None:intergenic 35.0%
TACATGGCAACTTTGGATAA+TGG - chr2_2:13541774-13541793 Msa0239270:CDS 35.0%
CTTTAATTCTCTGATCGACT+AGG - chr2_2:13542357-13542376 Msa0239270:intron 35.0%
GAAGAACAACTTGGTCAAAA+TGG + chr2_2:13542458-13542477 None:intergenic 35.0%
TTTGACCAAGTTGTTCTTCA+TGG - chr2_2:13542459-13542478 Msa0239270:intron 35.0%
!! TTCTTTGAAGCAGCTAACTT+GGG + chr2_2:13542624-13542643 None:intergenic 35.0%
! AAAACATCTGAGTTGAAGGA+AGG + chr2_2:13542654-13542673 None:intergenic 35.0%
! AGCAAAAACATCTGAGTTGA+AGG + chr2_2:13542658-13542677 None:intergenic 35.0%
! TGTGATAGTGATGTGATAAC+TGG - chr2_2:13542714-13542733 Msa0239270:CDS 35.0%
TCAATGAATCACCAAACTCG+GGG + chr2_2:13541619-13541638 None:intergenic 40.0%
GTCAATGAATCACCAAACTC+GGG + chr2_2:13541620-13541639 None:intergenic 40.0%
AAGACTAGATACGAGGTTGT+CGG - chr2_2:13541655-13541674 Msa0239270:CDS 40.0%
AGGTTGTCGGATAGATTGAT+TGG - chr2_2:13541668-13541687 Msa0239270:CDS 40.0%
!!! GTTTTGGGAAGAAGTAGTCA+CGG - chr2_2:13541744-13541763 Msa0239270:CDS 40.0%
TACCTCTTACATGGCAACTT+TGG - chr2_2:13541767-13541786 Msa0239270:CDS 40.0%
ATCCAAAGTTGCCATGTAAG+AGG + chr2_2:13541772-13541791 None:intergenic 40.0%
!! GTTTGTTGTTGAGAGTGAAG+TGG - chr2_2:13541796-13541815 Msa0239270:CDS 40.0%
ATCTTGTGATCAAAAGCTCC+TGG + chr2_2:13542167-13542186 None:intergenic 40.0%
GGTCAAAATGGAGATAGTTC+AGG + chr2_2:13542446-13542465 None:intergenic 40.0%
GGTTTCCATGAAGAACAACT+TGG + chr2_2:13542467-13542486 None:intergenic 40.0%
TACCAGCTTGAGTCATAAGT+TGG + chr2_2:13542557-13542576 None:intergenic 40.0%
ATTCTTGATACTGGTGCCTT+AGG - chr2_2:13542588-13542607 Msa0239270:intron 40.0%
!! CTTCTTTGAAGCAGCTAACT+TGG + chr2_2:13542625-13542644 None:intergenic 40.0%
TAGTGATGTGATAACTGGTG+AGG - chr2_2:13542719-13542738 Msa0239270:CDS 40.0%
!! GAGGTGTTGATCTTACAGAT+TGG - chr2_2:13542745-13542764 Msa0239270:CDS 40.0%
TCAATGAATCACCAAACTCG+GGG + chr2_2:13541619-13541638 None:intergenic 40.0%
GTCAATGAATCACCAAACTC+GGG + chr2_2:13541620-13541639 None:intergenic 40.0%
AAGACTAGATACGAGGTTGT+CGG - chr2_2:13541655-13541674 Msa0239270:CDS 40.0%
AGGTTGTCGGATAGATTGAT+TGG - chr2_2:13541668-13541687 Msa0239270:CDS 40.0%
!!! GTTTTGGGAAGAAGTAGTCA+CGG - chr2_2:13541744-13541763 Msa0239270:CDS 40.0%
TACCTCTTACATGGCAACTT+TGG - chr2_2:13541767-13541786 Msa0239270:CDS 40.0%
ATCCAAAGTTGCCATGTAAG+AGG + chr2_2:13541772-13541791 None:intergenic 40.0%
!! GTTTGTTGTTGAGAGTGAAG+TGG - chr2_2:13541796-13541815 Msa0239270:CDS 40.0%
ATCTTGTGATCAAAAGCTCC+TGG + chr2_2:13542167-13542186 None:intergenic 40.0%
GGTCAAAATGGAGATAGTTC+AGG + chr2_2:13542446-13542465 None:intergenic 40.0%
GGTTTCCATGAAGAACAACT+TGG + chr2_2:13542467-13542486 None:intergenic 40.0%
TACCAGCTTGAGTCATAAGT+TGG + chr2_2:13542557-13542576 None:intergenic 40.0%
ATTCTTGATACTGGTGCCTT+AGG - chr2_2:13542588-13542607 Msa0239270:intron 40.0%
!! CTTCTTTGAAGCAGCTAACT+TGG + chr2_2:13542625-13542644 None:intergenic 40.0%
TAGTGATGTGATAACTGGTG+AGG - chr2_2:13542719-13542738 Msa0239270:CDS 40.0%
!! GAGGTGTTGATCTTACAGAT+TGG - chr2_2:13542745-13542764 Msa0239270:CDS 40.0%
!!! AAGAGCACCAACCGAAGTTT+TGG - chr2_2:13541728-13541747 Msa0239270:CDS 45.0%
!! AGAGCACCAACCGAAGTTTT+GGG - chr2_2:13541729-13541748 Msa0239270:CDS 45.0%
CTTCTTCCCAAAACTTCGGT+TGG + chr2_2:13541738-13541757 None:intergenic 45.0%
TAGTCACGGTACCTCTTACA+TGG - chr2_2:13541758-13541777 Msa0239270:CDS 45.0%
TTGAGAGTGAAGTGGTTGTC+TGG - chr2_2:13541804-13541823 Msa0239270:CDS 45.0%
AAACGATCCACCACTGACAT+GGG - chr2_2:13542383-13542402 Msa0239270:intron 45.0%
CACCAACTTATGACTCAAGC+TGG - chr2_2:13542552-13542571 Msa0239270:intron 45.0%
! TCTGAGTTGAAGGAAGGCAT+TGG + chr2_2:13542648-13542667 None:intergenic 45.0%
CACATCACTATCACACCTTC+TGG + chr2_2:13542709-13542728 None:intergenic 45.0%
GATGTGATAACTGGTGAGGA+AGG - chr2_2:13542723-13542742 Msa0239270:CDS 45.0%
CTAAGATTGCGAGTAGCAGA+AGG - chr2_2:13542768-13542787 Msa0239270:CDS 45.0%
!!! AAGAGCACCAACCGAAGTTT+TGG - chr2_2:13541728-13541747 Msa0239270:CDS 45.0%
!! AGAGCACCAACCGAAGTTTT+GGG - chr2_2:13541729-13541748 Msa0239270:CDS 45.0%
CTTCTTCCCAAAACTTCGGT+TGG + chr2_2:13541738-13541757 None:intergenic 45.0%
TAGTCACGGTACCTCTTACA+TGG - chr2_2:13541758-13541777 Msa0239270:CDS 45.0%
TTGAGAGTGAAGTGGTTGTC+TGG - chr2_2:13541804-13541823 Msa0239270:CDS 45.0%
AAACGATCCACCACTGACAT+GGG - chr2_2:13542383-13542402 Msa0239270:intron 45.0%
CACCAACTTATGACTCAAGC+TGG - chr2_2:13542552-13542571 Msa0239270:intron 45.0%
! TCTGAGTTGAAGGAAGGCAT+TGG + chr2_2:13542648-13542667 None:intergenic 45.0%
CACATCACTATCACACCTTC+TGG + chr2_2:13542709-13542728 None:intergenic 45.0%
GATGTGATAACTGGTGAGGA+AGG - chr2_2:13542723-13542742 Msa0239270:CDS 45.0%
CTAAGATTGCGAGTAGCAGA+AGG - chr2_2:13542768-13542787 Msa0239270:CDS 45.0%
GTGGTTGTCTGGTTGAGAGA+AGG - chr2_2:13541815-13541834 Msa0239270:CDS 50.0%
TGTCTGGTTGAGAGAAGGAG+TGG - chr2_2:13541820-13541839 Msa0239270:CDS 50.0%
GAGAAGGAGTGGCGAAACAA+AGG - chr2_2:13541831-13541850 Msa0239270:CDS 50.0%
GAAACGATCCACCACTGACA+TGG - chr2_2:13542382-13542401 Msa0239270:intron 50.0%
CTAGCAGTTGACGTAGCATG+TGG - chr2_2:13542420-13542439 Msa0239270:intron 50.0%
CTTGGGTGCGTGATAACCTA+AGG + chr2_2:13542607-13542626 None:intergenic 50.0%
GTGATAACTGGTGAGGAAGG+AGG - chr2_2:13542726-13542745 Msa0239270:CDS 50.0%
GTGGTTGTCTGGTTGAGAGA+AGG - chr2_2:13541815-13541834 Msa0239270:CDS 50.0%
TGTCTGGTTGAGAGAAGGAG+TGG - chr2_2:13541820-13541839 Msa0239270:CDS 50.0%
GAGAAGGAGTGGCGAAACAA+AGG - chr2_2:13541831-13541850 Msa0239270:CDS 50.0%
GAAACGATCCACCACTGACA+TGG - chr2_2:13542382-13542401 Msa0239270:intron 50.0%
CTAGCAGTTGACGTAGCATG+TGG - chr2_2:13542420-13542439 Msa0239270:intron 50.0%
CTTGGGTGCGTGATAACCTA+AGG + chr2_2:13542607-13542626 None:intergenic 50.0%
GTGATAACTGGTGAGGAAGG+AGG - chr2_2:13542726-13542745 Msa0239270:CDS 50.0%
CTCTACGCCCATGTCAGTGG+TGG + chr2_2:13542393-13542412 None:intergenic 60.0%
CACCACTGACATGGGCGTAG+AGG - chr2_2:13542391-13542410 Msa0239270:intron 60.0%
AGCCTCTACGCCCATGTCAG+TGG + chr2_2:13542396-13542415 None:intergenic 60.0%
CTCTACGCCCATGTCAGTGG+TGG + chr2_2:13542393-13542412 None:intergenic 60.0%
CACCACTGACATGGGCGTAG+AGG - chr2_2:13542391-13542410 Msa0239270:intron 60.0%
AGCCTCTACGCCCATGTCAG+TGG + chr2_2:13542396-13542415 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 13541611 13542815 13541611 ID=Msa0239270;Name=Msa0239270
chr2_2 mRNA 13541611 13542815 13541611 ID=Msa0239270-mRNA-1;Parent=Msa0239270;Name=Msa0239270-mRNA-1;_AED=0.36;_eAED=0.38;_QI=0|0|0|1|1|1|2|0|182
chr2_2 exon 13542670 13542815 13542670 ID=Msa0239270-mRNA-1:exon:5649;Parent=Msa0239270-mRNA-1
chr2_2 exon 13541611 13542013 13541611 ID=Msa0239270-mRNA-1:exon:5648;Parent=Msa0239270-mRNA-1
chr2_2 CDS 13542670 13542815 13542670 ID=Msa0239270-mRNA-1:cds;Parent=Msa0239270-mRNA-1
chr2_2 CDS 13541611 13542013 13541611 ID=Msa0239270-mRNA-1:cds;Parent=Msa0239270-mRNA-1
Gene Sequence

>Msa0239270

ATGGTCCCCGAGTTTGGTGATTCATTGACTGCTGAGAATCTTACAAGACTAGATACGAGGTTGTCGGATAGATTGATTGGAGAGCTACATTTTCTTGATGATACTTTAGGAGCTATGAAGAGCACCAACCGAAGTTTTGGGAAGAAGCTCAAACTAGCAGTTGACGTAGCATGTGGCCTGAACTATCTCCATTTTGACCAAGTTGTTCTTCATGGAAACCTTAAAGCTATAAATGTGTTGTTAGATATCGCTGATATGAATGCACGCGTTGCTGATTATTGTCATCACCAACTTATGACTCAAGCTGGTATTATTGAAAAGATTCTTGATACTGGTGCCTTAGGTTATCACGCACCCAAGTTAGCTGCTTCAAAGAAGCCAATGCCTTCCTTCAACTCAGATGTTTTTGCTTTTGGCGTGATATTTTTTAAACTTTTAACCAGAAGGTGTGATAGTGATGTGATAACTGGTGAGGAAGGAGGTGTTGATCTTACAGATTGGCTAAGATTGCGAGTAGCAGAAGGTCGAAGCTCAAAATATTTGACGTAA
Protein sequence

>Msa0239270

MVPEFGDSLTAENLTRLDTRLSDRLIGELHFLDDTLGAMKSTNRSFGKKLKLAVDVACGLNYLHFDQVVLHGNLKAINVLLDIADMNARVADYCHHQLMTQAGIIEKILDTGALGYHAPKLAASKKPMPSFNSDVFAFGVIFFKLLTRRCDSDVITGEEGGVDLTDWLRLRVAEGRSSKYLT*