AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0242660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0242660 MtrunA17_Chr2g0294911 96.700 606 19 1 1 605 1 606 0.0 1217
Msa0242660 MtrunA17_Chr4g0067981 62.225 683 171 14 1 600 2 680 0.0 762
Msa0242660 MtrunA17_Chr1g0161431 59.091 308 117 3 25 325 8 313 1.93e-115 357
Msa0242660 MtrunA17_Chr3g0137101 48.189 359 151 7 26 356 6 357 1.97e-101 321
Msa0242660 MtrunA17_Chr3g0123131 58.462 260 92 3 28 277 13 266 2.79e-95 303
Msa0242660 MtrunA17_Chr8g0376881 57.874 254 105 1 26 277 23 276 2.22e-92 295
Msa0242660 MtrunA17_Chr1g0149841 44.444 378 174 9 24 385 1 358 3.57e-92 293
Msa0242660 MtrunA17_Chr7g0275891 49.827 289 120 5 24 291 1 285 6.60e-85 269
Msa0242660 MtrunA17_Chr2g0304841 42.973 370 178 7 24 363 14 380 3.34e-84 277
Msa0242660 MtrunA17_Chr4g0074401 42.703 370 172 6 17 368 6 353 2.19e-82 270
Msa0242660 MtrunA17_Chr1g0149851 52.045 269 110 3 24 273 1 269 1.96e-79 261
Msa0242660 MtrunA17_Chr4g0074411 44.706 340 149 8 22 346 12 327 6.96e-79 261
Msa0242660 MtrunA17_Chr7g0275401 47.751 289 139 4 24 303 1 286 4.12e-75 241
Msa0242660 MtrunA17_Chr4g0074421 51.515 198 73 3 78 264 2 187 3.41e-59 196
Msa0242660 MtrunA17_Chr3g0134461 47.034 236 76 2 25 259 5 192 5.57e-59 195
Msa0242660 MtrunA17_Chr3g0134481 43.882 237 75 2 25 261 5 183 4.55e-57 190
Msa0242660 MtrunA17_Chr4g0009851 43.096 239 112 5 28 266 11 225 1.63e-56 193
Msa0242660 MtrunA17_Chr1g0149831 50.739 203 79 4 87 278 1 193 4.20e-56 189
Msa0242660 MtrunA17_Chr4g0009901 42.917 240 113 4 28 267 10 225 2.08e-55 189
Msa0242660 MtrunA17_Chr6g0471041 38.843 242 120 6 27 266 13 228 4.56e-45 165
Msa0242660 MtrunA17_Chr4g0009981 41.753 194 90 1 73 266 2 172 2.37e-44 158
Msa0242660 MtrunA17_Chr8g0391911 34.766 256 141 6 28 269 9 252 2.04e-35 134
Msa0242660 MtrunA17_Chr6g0473731 37.838 185 86 5 108 284 10 173 3.76e-29 120
Msa0242660 MtrunA17_Chr8g0383961 42.754 138 76 1 27 164 13 147 1.48e-28 115
Msa0242660 MtrunA17_Chr8g0350761 30.000 270 165 6 30 277 19 286 1.35e-27 116
Msa0242660 MtrunA17_Chr4g0011071 35.220 159 80 3 78 236 39 174 3.91e-27 108
Msa0242660 MtrunA17_Chr4g0031961 47.458 118 47 3 186 295 99 209 5.31e-24 103
Msa0242660 MtrunA17_Chr4g0009991 39.286 140 61 2 120 259 1 116 7.97e-23 94.4
Msa0242660 MtrunA17_Chr3g0127941 36.875 160 94 4 32 186 56 213 3.50e-22 101
Msa0242660 MtrunA17_Chr7g0276361 38.971 136 74 2 29 158 11 143 1.20e-21 99.4
Msa0242660 MtrunA17_Chr6g0456631 36.220 127 78 2 26 150 7 132 1.30e-21 92.4
Msa0242660 MtrunA17_Chr4g0030271 35.333 150 91 3 6 150 62 210 3.30e-21 98.2
Msa0242660 MtrunA17_Chr7g0255711 57.971 69 29 0 203 271 84 152 3.87e-21 94.4
Msa0242660 MtrunA17_Chr1g0181811 39.344 122 71 1 32 150 85 206 8.73e-21 97.1
Msa0242660 MtrunA17_Chr3g0091641 35.971 139 84 2 19 152 9 147 1.63e-20 95.5
Msa0242660 MtrunA17_Chr4g0061021 35.507 138 84 2 18 152 21 156 5.85e-19 90.9
Msa0242660 MtrunA17_Chr8g0345901 39.344 122 67 3 34 150 38 157 9.02e-19 90.5
Msa0242660 MtrunA17_Chr5g0400241 37.903 124 68 2 145 268 255 369 7.35e-17 84.0
Msa0242660 MtrunA17_Chr5g0400241 37.008 127 76 2 28 153 14 137 6.85e-14 74.7
Msa0242660 MtrunA17_Chr1g0198601 36.552 145 78 4 198 330 199 341 3.91e-16 80.5
Msa0242660 MtrunA17_Chr5g0423031 45.679 81 43 1 28 108 10 89 6.10e-16 76.3
Msa0242660 MtrunA17_Chr4g0010001 44.444 81 44 1 28 108 10 89 6.51e-16 73.6
Msa0242660 MtrunA17_Chr5g0417521 55.714 70 24 2 206 274 11 74 4.66e-15 77.0
Msa0242660 MtrunA17_Chr4g0040481 41.379 87 46 1 209 295 157 238 4.67e-15 77.8
Msa0242660 MtrunA17_Chr4g0054981 47.887 71 37 0 209 279 309 379 7.68e-15 77.8
Msa0242660 MtrunA17_Chr4g0054981 36.220 127 77 2 28 153 14 137 1.65e-13 73.6
Msa0242660 MtrunA17_Chr6g0469321 63.462 52 19 0 227 278 71 122 3.68e-14 71.2
Msa0242660 MtrunA17_Chr4g0042451 55.932 59 25 1 206 263 13 71 7.59e-14 73.2
Msa0242660 MtrunA17_Chr2g0324891 53.333 60 28 0 209 268 160 219 7.89e-14 73.9
Msa0242660 MtrunA17_Chr2g0321461 55.932 59 25 1 206 263 41 99 8.23e-14 73.6
Msa0242660 MtrunA17_Chr6g0461641 51.667 60 28 1 205 263 37 96 2.00e-13 72.4
Msa0242660 MtrunA17_Chr5g0422621 30.220 182 115 5 151 325 133 309 2.28e-13 72.0
Msa0242660 MtrunA17_Chr5g0402631 50.000 72 35 1 207 277 211 282 8.42e-13 70.1
Msa0242660 MtrunA17_Chr2g0310011 54.237 59 26 1 206 263 31 89 2.27e-12 68.9
Msa0242660 MtrunA17_Chr8g0356501 54.237 59 26 1 206 263 33 91 2.92e-12 68.6
Msa0242660 MtrunA17_Chr4g0045361 50.746 67 32 1 207 272 271 337 3.03e-12 68.9
Msa0242660 MtrunA17_Chr1g0197171 52.308 65 27 2 203 263 34 98 7.92e-12 66.6
Msa0242660 MtrunA17_Chr7g0274471 59.259 54 21 1 210 262 218 271 1.17e-11 67.0
Msa0242660 MtrunA17_Chr4g0062921 34.074 135 77 4 166 295 188 315 1.25e-11 67.0
Msa0242660 MtrunA17_Chr7g0261911 49.206 63 31 1 206 267 258 320 1.95e-11 66.6
Msa0242660 MtrunA17_Chr1g0190301 50.847 59 28 1 205 262 170 228 5.60e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0242660 AT3G16857.2 46.999 683 241 21 21 600 26 690 2.14e-174 511
Msa0242660 AT3G16857.1 46.364 660 234 20 21 577 26 668 5.21e-163 481
Msa0242660 AT3G16857.3 44.671 638 232 21 66 600 1 620 1.13e-143 430
Msa0242660 AT2G01760.1 46.053 380 159 9 24 373 3 366 9.03e-96 298
Msa0242660 AT2G25180.1 59.924 262 93 3 26 281 11 266 9.05e-96 305
Msa0242660 AT2G01760.2 42.892 408 159 10 24 373 3 394 1.49e-89 283
Msa0242660 AT4G31920.1 47.143 350 142 8 24 354 9 334 1.56e-87 283
Msa0242660 AT1G67710.1 49.702 336 128 9 29 333 8 333 5.53e-86 278
Msa0242660 AT5G58080.1 46.875 320 128 7 26 324 12 310 6.33e-79 262
Msa0242660 AT2G01760.3 42.271 317 137 9 87 373 1 301 2.57e-62 209
Msa0242660 AT1G67710.2 45.683 278 110 9 87 333 1 268 1.64e-56 197
Msa0242660 AT5G49240.1 42.915 247 134 3 18 259 28 272 9.27e-54 185
Msa0242660 AT1G49190.1 35.254 295 149 7 27 296 29 306 8.74e-46 167
Msa0242660 AT3G62670.1 39.407 236 128 5 33 262 42 268 1.96e-45 167
Msa0242660 AT1G67710.3 41.473 258 110 9 107 333 12 259 1.16e-40 154
Msa0242660 AT5G07210.2 33.955 268 145 6 32 269 16 281 7.71e-39 151
Msa0242660 AT5G07210.1 32.971 276 145 7 32 269 16 289 2.77e-36 144
Msa0242660 AT2G27070.1 32.103 271 147 7 32 268 16 283 3.03e-33 134
Msa0242660 AT1G49190.2 44.800 125 69 0 27 151 29 153 2.86e-29 123
Msa0242660 AT5G59570.2 52.273 88 42 0 194 281 125 212 3.48e-24 103
Msa0242660 AT5G59570.1 52.273 88 42 0 194 281 125 212 3.48e-24 103
Msa0242660 AT3G46640.2 46.364 110 52 1 194 303 128 230 3.63e-23 101
Msa0242660 AT3G46640.1 46.364 110 52 1 194 303 128 230 3.63e-23 101
Msa0242660 AT2G46790.1 40.650 123 68 2 32 150 37 158 4.60e-23 103
Msa0242660 AT2G46790.3 40.650 123 68 2 32 150 37 158 4.80e-23 102
Msa0242660 AT3G46640.3 49.020 102 45 1 194 295 128 222 6.02e-23 100
Msa0242660 AT5G24470.1 39.200 125 71 2 31 151 49 172 5.08e-21 97.4
Msa0242660 AT5G60100.7 39.837 123 69 2 32 150 64 185 5.50e-21 96.3
Msa0242660 AT5G60100.6 39.837 123 69 2 32 150 64 185 5.50e-21 96.3
Msa0242660 AT5G60100.4 39.837 123 69 2 32 150 64 185 5.50e-21 96.3
Msa0242660 AT5G60100.5 39.837 123 69 2 32 150 64 185 6.82e-21 96.7
Msa0242660 AT5G60100.3 39.837 123 69 2 32 150 64 185 6.82e-21 96.7
Msa0242660 AT5G60100.1 39.837 123 69 2 32 150 64 185 6.82e-21 96.7
Msa0242660 AT2G40970.1 60.606 66 26 0 203 268 98 163 8.80e-21 92.4
Msa0242660 AT5G60100.2 39.837 123 69 2 32 150 64 185 1.71e-20 95.5
Msa0242660 AT5G05090.1 59.091 66 27 0 203 268 74 139 2.42e-20 91.7
Msa0242660 AT3G10760.1 59.091 66 27 0 203 268 98 163 3.78e-20 92.4
Msa0242660 AT5G61380.1 38.710 124 73 1 32 152 19 142 4.13e-20 95.1
Msa0242660 AT5G02810.1 40.164 122 70 1 32 150 78 199 5.38e-20 94.7
Msa0242660 AT5G44190.2 50.649 77 38 0 203 279 140 216 8.44e-18 85.9
Msa0242660 AT5G44190.1 50.649 77 38 0 203 279 140 216 1.06e-17 85.9
Msa0242660 AT2G20570.1 39.823 113 63 2 170 277 108 220 4.33e-16 81.3
Msa0242660 AT2G20570.2 38.060 134 77 2 145 277 108 236 2.15e-14 76.3
Msa0242660 AT3G04030.1 51.282 78 30 3 206 282 41 111 3.54e-14 75.1
Msa0242660 AT3G04030.3 51.282 78 30 3 206 282 41 111 4.17e-14 75.1
Msa0242660 AT5G18240.2 50.000 82 37 3 205 285 40 118 5.53e-14 74.7
Msa0242660 AT5G18240.3 50.000 82 37 3 205 285 40 118 5.53e-14 74.7
Msa0242660 AT5G18240.5 50.000 82 37 3 205 285 40 118 5.82e-14 74.7
Msa0242660 AT5G18240.1 50.000 82 37 3 205 285 40 118 5.89e-14 74.7
Msa0242660 AT5G18240.4 50.000 82 37 3 205 285 40 118 5.89e-14 74.7
Msa0242660 AT3G04030.2 48.052 77 33 2 206 282 41 110 4.88e-13 71.6
Msa0242660 AT4G18020.8 36.029 136 78 3 26 154 12 145 5.18e-13 72.0
Msa0242660 AT4G18020.8 51.613 62 29 1 207 268 294 354 6.93e-13 71.6
Msa0242660 AT4G18020.11 36.029 136 78 3 26 154 12 145 5.18e-13 72.0
Msa0242660 AT4G18020.11 51.613 62 29 1 207 268 294 354 6.93e-13 71.6
Msa0242660 AT4G18020.4 36.029 136 78 3 26 154 12 145 5.18e-13 72.0
Msa0242660 AT4G18020.4 51.613 62 29 1 207 268 294 354 6.93e-13 71.6
Msa0242660 AT4G18020.5 36.029 136 78 3 26 154 12 145 5.18e-13 72.0
Msa0242660 AT4G18020.5 51.613 62 29 1 207 268 294 354 6.93e-13 71.6
Msa0242660 AT4G18020.10 36.029 136 78 3 26 154 12 145 5.18e-13 72.0
Msa0242660 AT4G18020.10 51.613 62 29 1 207 268 294 354 6.93e-13 71.6
Msa0242660 AT4G18020.7 36.029 136 78 3 26 154 12 145 5.18e-13 72.0
Msa0242660 AT4G18020.7 51.613 62 29 1 207 268 294 354 6.93e-13 71.6
Msa0242660 AT4G18020.3 36.029 136 78 3 26 154 12 145 7.01e-13 72.0
Msa0242660 AT4G18020.3 51.613 62 29 1 207 268 294 354 7.38e-13 71.6
Msa0242660 AT4G18020.2 36.029 136 78 3 26 154 12 145 7.01e-13 72.0
Msa0242660 AT4G18020.2 51.613 62 29 1 207 268 294 354 7.38e-13 71.6
Msa0242660 AT4G18020.1 36.029 136 78 3 26 154 12 145 7.01e-13 72.0
Msa0242660 AT4G18020.1 51.613 62 29 1 207 268 294 354 7.38e-13 71.6
Msa0242660 AT4G18020.6 36.029 136 78 3 26 154 12 145 7.01e-13 72.0
Msa0242660 AT4G18020.6 51.613 62 29 1 207 268 294 354 7.38e-13 71.6
Msa0242660 AT4G18020.9 36.029 136 78 3 26 154 12 145 7.01e-13 72.0
Msa0242660 AT4G18020.9 51.613 62 29 1 207 268 294 354 7.38e-13 71.6
Msa0242660 AT1G69580.1 54.237 59 26 1 206 263 30 88 7.35e-13 70.5
Msa0242660 AT1G69580.2 54.237 59 26 1 206 263 30 88 7.54e-13 70.5
Msa0242660 AT3G24120.2 52.308 65 27 2 203 263 31 95 3.01e-12 68.2
Msa0242660 AT3G24120.1 52.308 65 27 2 203 263 31 95 3.31e-12 68.2
Msa0242660 AT1G79430.2 53.333 60 27 1 205 263 29 88 3.65e-12 68.6
Msa0242660 AT3G25790.1 38.655 119 61 4 214 330 200 308 3.86e-12 68.6
Msa0242660 AT4G13640.2 52.308 65 27 2 203 263 27 91 7.37e-12 67.0
Msa0242660 AT4G13640.1 52.308 65 27 2 203 263 27 91 7.48e-12 67.0
Msa0242660 AT1G49560.1 37.383 107 59 2 209 307 191 297 1.98e-11 66.2
Msa0242660 AT3G12730.1 49.275 69 28 2 202 263 9 77 2.72e-11 64.3
Msa0242660 AT1G25550.1 45.070 71 38 1 198 267 197 267 3.78e-11 65.5
Msa0242660 AT5G29000.4 45.714 70 35 2 196 262 215 284 5.82e-11 64.7
Msa0242660 AT1G68670.1 45.679 81 42 2 194 272 198 278 5.98e-11 65.1
Msa0242660 AT2G03500.1 47.059 68 35 1 207 273 230 297 8.68e-11 64.7

Find 152 sgRNAs with CRISPR-Local

Find 239 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGCTTCATCAACAGTTTA+TGG 0.134096 2_2:-18971547 Msa0242660:CDS
CTTCAAACCCTTTCAGCTTC+TGG 0.197138 2_2:-18970656 Msa0242660:CDS
CCTTATCTCCGACCAGTTTC+CGG 0.220153 2_2:-18972588 Msa0242660:CDS
GTTGTTGTTGTTGAGTTTCA+TGG 0.237423 2_2:+18972655 None:intergenic
GATGTCTTGCCATCGATTTC+AGG 0.243060 2_2:-18970056 Msa0242660:CDS
GAAGCTACTTCGGGAATTAA+AGG 0.286940 2_2:-18970203 Msa0242660:CDS
AGTACGCAAGAGTCAATATT+TGG 0.295173 2_2:-18970704 Msa0242660:CDS
TATGGATGGATTCAAACTTT+TGG 0.295920 2_2:-18972036 Msa0242660:CDS
ATTAAACTAAGAAAATTAAT+TGG 0.296217 2_2:+18972787 None:intergenic
TGAGGTGTTGACCAACATTT+TGG 0.304132 2_2:+18969967 None:intergenic
TGCAAGTTACAACTGTTATA+AGG 0.306416 2_2:-18972845 Msa0242660:five_prime_UTR
CACCAGTCTGCTCAATCCTT+TGG 0.321223 2_2:-18970461 Msa0242660:CDS
GAGAACTCAAAACTAAGATT+TGG 0.328580 2_2:-18970578 Msa0242660:CDS
ACTTCGGGAATTAAAGGATT+AGG 0.335057 2_2:-18970197 Msa0242660:CDS
AAGAAGATGAAGCAGATGAT+AGG 0.337750 2_2:-18971614 Msa0242660:CDS
AAGAAAATTAATTGGTTTGT+GGG 0.341269 2_2:+18972795 None:intergenic
TTGAGCAGACTGGTGAAAAT+TGG 0.352834 2_2:+18970469 None:intergenic
TTCAGCTTCTGGCCAGTTCT+CGG 0.367341 2_2:-18970645 Msa0242660:CDS
CGACGTGCATATGCCTGATA+TGG 0.368808 2_2:-18972054 Msa0242660:CDS
TCGAGGCTTTGAAGAATATA+TGG 0.369544 2_2:-18971788 Msa0242660:CDS
CTTCGGGAATTAAAGGATTA+GGG 0.371380 2_2:-18970196 Msa0242660:CDS
ATATCCAGTAGCAGTATAAA+AGG 0.377177 2_2:-18970239 Msa0242660:CDS
AAACAATGCTTACTAATAAC+AGG 0.383212 2_2:+18971987 None:intergenic
CTATTGACTGATATTCCATT+TGG 0.387671 2_2:+18970287 None:intergenic
GGCAAGAGTGAAAGAATTCC+AGG 0.393464 2_2:-18969915 Msa0242660:CDS
TAAGAAAATTAATTGGTTTG+TGG 0.396703 2_2:+18972794 None:intergenic
TTCGGAAGAGAAAGAATAAC+TGG 0.397525 2_2:-18971755 Msa0242660:CDS
CTAGACTTTGCGCCGAGAAC+TGG 0.398375 2_2:+18970633 None:intergenic
TCATTTAGCATCTGACCTCT+TGG 0.399993 2_2:+18970365 None:intergenic
GATAATGAGTTTGACTTTGA+TGG 0.400817 2_2:-18969704 Msa0242660:CDS
CACGGAGGCCCGCCGGAAAC+TGG 0.401569 2_2:+18972576 None:intergenic
AAACGTTGCCAGTCATCTTC+AGG 0.402049 2_2:-18971174 Msa0242660:intron
TAACAGGATCTGTTATAAAC+TGG 0.403457 2_2:+18969662 None:intergenic
AACCTCAGCTCTCTTGCATT+TGG 0.403620 2_2:+18972127 None:intergenic
ATTTGCTAATCCCCAATCAT+TGG 0.406561 2_2:+18970133 None:intergenic
CATGAGTGTGTTTCTGAACC+AGG 0.407623 2_2:-18969844 Msa0242660:intron
TGCTTCGGCTCAATGTTGAT+AGG 0.409175 2_2:+18970497 None:intergenic
CAAAGAAGCCTCGAGTTGTT+TGG 0.412556 2_2:-18971575 Msa0242660:CDS
TTTCATGCGGAAGCTACTTC+GGG 0.415768 2_2:-18970212 Msa0242660:CDS
CCATCAGAAGTCCAATGATT+GGG 0.416956 2_2:-18970144 Msa0242660:CDS
TATTCAAGTTACTTTGCTGC+TGG 0.418842 2_2:+18970738 None:intergenic
TGAAAATTGGCAAACTGCTT+CGG 0.421606 2_2:+18970482 None:intergenic
ATGGAAATGATGAATGTTCC+TGG 0.426157 2_2:-18971209 Msa0242660:CDS
AGTTTGAATCCATCCATATC+AGG 0.428415 2_2:+18972041 None:intergenic
ACATCAATGTTTCCTTGTAA+AGG 0.437888 2_2:+18970074 None:intergenic
AAGATTGAAGACCTTCATAA+AGG 0.449070 2_2:+18972492 None:intergenic
CTGAGCCATCTGCGTCAACC+AGG 0.458938 2_2:+18970394 None:intergenic
ATAAGGTTGCCGGAGTTTGA+TGG 0.460200 2_2:+18972605 None:intergenic
TATGGCTGTTGTGAATCAAT+TGG 0.460490 2_2:-18971529 Msa0242660:CDS
TCCATCAGAAGTCCAATGAT+TGG 0.463174 2_2:-18970145 Msa0242660:CDS
CGTAATGAATTGTCAACAAA+AGG 0.464558 2_2:+18969939 None:intergenic
TGATTAGAAAGAGAGCTAAC+AGG 0.464856 2_2:+18969646 None:intergenic
TGGAAAGATCTGAAGCAATC+TGG 0.465336 2_2:-18971735 Msa0242660:CDS
CTCTTGACTTTGATCAGAGA+AGG 0.465507 2_2:+18969872 None:intergenic
TTCTCGCAGCACAAAGGCGT+TGG 0.468445 2_2:-18969734 Msa0242660:intron
CCGGAAACTGGTCGGAGATA+AGG 0.470882 2_2:+18972588 None:intergenic
CCCAATCATTGGACTTCTGA+TGG 0.470889 2_2:+18970144 None:intergenic
TTGTCGAGTCTGTTGGATAG+AGG 0.472904 2_2:+18970019 None:intergenic
ATGGCTGTTGTGAATCAATT+GGG 0.473696 2_2:-18971528 Msa0242660:CDS
ATGCAACCTACAGGACCAAA+TGG 0.473881 2_2:-18970302 Msa0242660:CDS
ACATATGAGCAGCAGTAAAC+CGG 0.474063 2_2:-18970540 Msa0242660:CDS
ATTTCATGCGGAAGCTACTT+CGG 0.474676 2_2:-18970213 Msa0242660:CDS
CTAGCCAAGCTTCAAGCAGC+AGG 0.477941 2_2:-18970614 Msa0242660:CDS
GGAACATATTGGATTGGAGA+TGG 0.480393 2_2:-18972015 Msa0242660:CDS
AGAAGATGAAGCAGATGATA+GGG 0.481453 2_2:-18971613 Msa0242660:CDS
CAACATGCAAATCCTTTACA+AGG 0.491783 2_2:-18970086 Msa0242660:CDS
TTCCCTCTCAGATGCACTCC+GGG 0.493223 2_2:-18970263 Msa0242660:CDS
GTAGGTTGCATTAAAGATGA+TGG 0.494028 2_2:+18970314 None:intergenic
TACTCATCTTCAACGAATGA+AGG 0.499328 2_2:-18971663 Msa0242660:CDS
GATATTCCATTTGGTCCTGT+AGG 0.499380 2_2:+18970296 None:intergenic
CAAAACTAAGATTTGGAGAT+GGG 0.507173 2_2:-18970571 Msa0242660:CDS
CAAGGATCATGAGGCAAGTA+GGG 0.508406 2_2:+18972534 None:intergenic
CTGGTCGGAGATAAGGTTGC+CGG 0.509887 2_2:+18972595 None:intergenic
ATAATGAGTTTGACTTTGAT+GGG 0.511882 2_2:-18969703 Msa0242660:CDS
ATTCAAGTTACTTTGCTGCT+GGG 0.515195 2_2:+18970739 None:intergenic
GTTGAGAAGCATCATATGTC+AGG 0.515544 2_2:+18970105 None:intergenic
TCTTGACTTTGATCAGAGAA+GGG 0.515547 2_2:+18969873 None:intergenic
GATTGGGGATTAGCAAATAC+AGG 0.520281 2_2:-18970128 Msa0242660:CDS
TTCAAAGCCTCGATTCGCAC+AGG 0.523205 2_2:+18971798 None:intergenic
ATATATGGCAGCATGTGGTT+CGG 0.524503 2_2:-18971773 Msa0242660:CDS
TCAAGGATCATGAGGCAAGT+AGG 0.525643 2_2:+18972533 None:intergenic
GCTTCGGCTCAATGTTGATA+GGG 0.528322 2_2:+18970498 None:intergenic
CCGCATATTTGCGCTGCCAA+AGG 0.530696 2_2:+18970445 None:intergenic
AAACCGGTGAACTTTCTTCA+CGG 0.535622 2_2:-18970524 Msa0242660:CDS
ATACAAATTCTCATGCTACA+AGG 0.536556 2_2:-18970340 Msa0242660:CDS
CTTTGGCAGCGCAAATATGC+GGG 0.536705 2_2:-18970444 Msa0242660:CDS
TCAAAACTAAGATTTGGAGA+TGG 0.536886 2_2:-18970572 Msa0242660:CDS
ATCTCCGACCAGTTTCCGGC+GGG 0.537771 2_2:-18972584 Msa0242660:CDS
TTGTATCTTCGAAGGTTGAG+CGG 0.540940 2_2:-18970767 Msa0242660:CDS
GAACTGGCCAGAAGCTGAAA+GGG 0.541735 2_2:+18970649 None:intergenic
AAGCCCCTGGTTGACGCAGA+TGG 0.544982 2_2:-18970399 Msa0242660:CDS
AATACCGTTTGTATCTTCGA+AGG 0.548651 2_2:-18970775 Msa0242660:CDS
TTAATCTCGATGTTTGTACC+TGG 0.554637 2_2:+18969897 None:intergenic
TCATCTTTAATGCAACCTAC+AGG 0.556863 2_2:-18970311 Msa0242660:CDS
TTAGTGATGTCGGCTGATGA+TGG 0.559693 2_2:-18971876 Msa0242660:intron
TTTCCCTCTCAGATGCACTC+CGG 0.561462 2_2:-18970264 Msa0242660:CDS
ATATCATACCTGAAGATGAC+TGG 0.562314 2_2:+18971166 None:intergenic
TGAGCCATCTGCGTCAACCA+GGG 0.564665 2_2:+18970395 None:intergenic
AGAACTGGCCAGAAGCTGAA+AGG 0.565004 2_2:+18970648 None:intergenic
TAGTTGCCAATGTAAATAGT+TGG 0.567960 2_2:-18969623 Msa0242660:three_prime_UTR
GGAACACCAACTATTTACAT+TGG 0.570285 2_2:+18969617 None:intergenic
ACAAGTACACGGAGGCCCGC+CGG 0.571204 2_2:+18972569 None:intergenic
TGACTCTTGCGTACTCATGA+AGG 0.572243 2_2:+18970712 None:intergenic
ATTCCGTGAAGAAAGTTCAC+CGG 0.576781 2_2:+18970521 None:intergenic
TGCCAAAGGATTGAGCAGAC+TGG 0.579512 2_2:+18970459 None:intergenic
CAAGGTGATCACCAAAATGT+TGG 0.586348 2_2:-18969978 Msa0242660:CDS
GCAAGAGCTCAAAGAAGAGG+AGG 0.587165 2_2:-18971641 Msa0242660:CDS
CTTCTCTGATCAAAGTCAAG+AGG 0.587257 2_2:-18969871 Msa0242660:CDS
GAGCCATCTGCGTCAACCAG+GGG 0.591634 2_2:+18970396 None:intergenic
CCACGATGGCCTGAAATCGA+TGG 0.592221 2_2:+18970047 None:intergenic
AAGTCCTGCTGCTTGAAGCT+TGG 0.592461 2_2:+18970610 None:intergenic
TGTCAACAAAAGGTGTAATG+AGG 0.594092 2_2:+18969949 None:intergenic
AGATGAGTAATTAGCATCAG+TGG 0.594629 2_2:+18971677 None:intergenic
AAGATCAATTTCATTTACAG+AGG 0.595752 2_2:+18970676 None:intergenic
CCTTTGGCAGCGCAAATATG+CGG 0.597795 2_2:-18970445 Msa0242660:CDS
TGGTGAAGTTGAGGTTGACA+TGG 0.599570 2_2:+18972625 None:intergenic
CTTGACTTTGATCAGAGAAG+GGG 0.600312 2_2:+18969874 None:intergenic
TACCCGGAGTGCATCTGAGA+GGG 0.609734 2_2:+18970261 None:intergenic
AATAGAGATGGTATGAGTCA+AGG 0.612883 2_2:-18969996 Msa0242660:CDS
AATCAAACCTGTGCGAATCG+AGG 0.614923 2_2:-18971805 Msa0242660:CDS
TCAACTTCACCATCAAACTC+CGG 0.617812 2_2:-18972614 Msa0242660:CDS
TATCTCCGACCAGTTTCCGG+CGG 0.618522 2_2:-18972585 Msa0242660:CDS
GTGCATATGCCTGATATGGA+TGG 0.621582 2_2:-18972050 Msa0242660:CDS
CAGACTCGACAAAATAGAGA+TGG 0.622886 2_2:-18970008 Msa0242660:CDS
CATCAGAAGTCCAATGATTG+GGG 0.623338 2_2:-18970143 Msa0242660:CDS
CCATCGATTTCAGGCCATCG+TGG 0.623771 2_2:-18970047 Msa0242660:CDS
GCATTCTTTCAAGGATCATG+AGG 0.625199 2_2:+18972525 None:intergenic
CTGCTGCAAAGAGAAGCCCC+TGG 0.625822 2_2:-18970412 Msa0242660:CDS
TGGAAATGATGAATGTTCCT+GGG 0.628797 2_2:-18971208 Msa0242660:CDS
AAAGATCTGAAGCAATCTGG+TGG 0.628824 2_2:-18971732 Msa0242660:CDS
GAAGAATATATGGCAGCATG+TGG 0.629609 2_2:-18971778 Msa0242660:CDS
TCAACCTTCGAAGATACAAA+CGG 0.630642 2_2:+18970771 None:intergenic
TTCGGGAATTAAAGGATTAG+GGG 0.632056 2_2:-18970195 Msa0242660:CDS
TTGTATTTCTCGCAGCACAA+AGG 0.634173 2_2:-18969740 Msa0242660:intron
AAGGCAAGAGCTCAAAGAAG+AGG 0.635070 2_2:-18971644 Msa0242660:CDS
ACGCAAGAGTCAATATTTGG+AGG 0.635081 2_2:-18970701 Msa0242660:CDS
TCGGGAATTAAAGGATTAGG+GGG 0.636049 2_2:-18970194 Msa0242660:CDS
TCAATAGACCAAACAACTCG+AGG 0.639848 2_2:+18971567 None:intergenic
GAGGCCCGCCGGAAACTGGT+CGG 0.643720 2_2:+18972580 None:intergenic
GGAGTTTGATGGTGAAGTTG+AGG 0.651951 2_2:+18972616 None:intergenic
CATCATCAACAACAAGTACA+CGG 0.653506 2_2:+18972558 None:intergenic
GAATGATGATTAGATTCATG+TGG 0.654143 2_2:+18969596 None:intergenic
CAAGAGCTCAAAGAAGAGGA+GGG 0.663100 2_2:-18971640 Msa0242660:CDS
TACCAAATGCAAGAGAGCTG+AGG 0.664673 2_2:-18972129 Msa0242660:CDS
TTTGTTGACAATTCATTACG+GGG 0.665334 2_2:-18969936 Msa0242660:CDS
ATACTGCTACTGGATATACC+CGG 0.681469 2_2:+18970245 None:intergenic
TATAAAAGGTGCATTTCATG+CGG 0.685544 2_2:-18970225 Msa0242660:CDS
ATACCCGGAGTGCATCTGAG+AGG 0.700149 2_2:+18970260 None:intergenic
ATGGCTCAGTCTCAACCAAG+AGG 0.702081 2_2:-18970380 Msa0242660:CDS
GTAATGAAAGGTGTGACACA+CGG 0.719143 2_2:-18971843 Msa0242660:CDS
ATAAACTGGAATGTTGTCTG+CGG 0.732217 2_2:+18969676 None:intergenic
CATCAACAACAAGTACACGG+AGG 0.818660 2_2:+18972561 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAACTAAGAAAATTAAT+TGG + chr2_2:18969536-18969555 None:intergenic 10.0%
!!! GTTTTTTTTTTTTATTGATT+TGG - chr2_2:18970352-18970371 Msa0242660:CDS 10.0%
!!! GATTTTTATATGTAATGTTA+AGG - chr2_2:18971025-18971044 Msa0242660:intron 15.0%
!! CAAGTAAACATAAATATTTA+GGG + chr2_2:18972523-18972542 None:intergenic 15.0%
!! TTAGTTTATGTAAAAATTTC+AGG - chr2_2:18972796-18972815 Msa0242660:five_prime_UTR 15.0%
!!! AAGAAAATTAATTGGTTTGT+GGG + chr2_2:18969528-18969547 None:intergenic 20.0%
!!! TAAGAAAATTAATTGGTTTG+TGG + chr2_2:18969529-18969548 None:intergenic 20.0%
!!! TTTTTTTGTTGTCTACTATT+TGG - chr2_2:18969633-18969652 Msa0242660:three_prime_UTR 20.0%
!!! TTTTAACACAAATTCATGAT+GGG + chr2_2:18969873-18969892 None:intergenic 20.0%
!!! TTTTTAACACAAATTCATGA+TGG + chr2_2:18969874-18969893 None:intergenic 20.0%
!! AAAAACTCAATCAAACAAAA+GGG + chr2_2:18969955-18969974 None:intergenic 20.0%
!! AAAAAACTCAATCAAACAAA+AGG + chr2_2:18969956-18969975 None:intergenic 20.0%
!! CCAGAACTAAAAATAAAAAT+GGG + chr2_2:18969987-18970006 None:intergenic 20.0%
!! ACCAGAACTAAAAATAAAAA+TGG + chr2_2:18969988-18970007 None:intergenic 20.0%
!!! TAAGTGTTTTTTTCAACTAT+TGG - chr2_2:18970069-18970088 Msa0242660:CDS 20.0%
!! ATATCTTGGAAAAACTTTAA+GGG + chr2_2:18970920-18970939 None:intergenic 20.0%
!! TATATCTTGGAAAAACTTTA+AGG + chr2_2:18970921-18970940 None:intergenic 20.0%
!! AGAAATATTAAGATTCTATG+GGG + chr2_2:18970993-18971012 None:intergenic 20.0%
!! CAGAAATATTAAGATTCTAT+GGG + chr2_2:18970994-18971013 None:intergenic 20.0%
!!! ATCATTTCCATAATCTTTTT+AGG + chr2_2:18971102-18971121 None:intergenic 20.0%
!! CTTCTAAACTAACTTATTAT+AGG + chr2_2:18971245-18971264 None:intergenic 20.0%
!!! ATAATAAGTTAGTTTAGAAG+TGG - chr2_2:18971245-18971264 Msa0242660:intron 20.0%
!!! TCTAATAACATCTAGAATAT+CGG + chr2_2:18971323-18971342 None:intergenic 20.0%
!! CCAAGTAAACATAAATATTT+AGG + chr2_2:18972524-18972543 None:intergenic 20.0%
!! CCTAAATATTTATGTTTACT+TGG - chr2_2:18972521-18972540 Msa0242660:CDS 20.0%
!! AGTGAAAAGTGATGATTTTA+TGG + chr2_2:18969577-18969596 None:intergenic 25.0%
!!! CCCATTTTTATTTTTAGTTC+TGG - chr2_2:18969984-18970003 Msa0242660:CDS 25.0%
!! GCTTTTGATATGATTTAGTA+TGG - chr2_2:18970033-18970052 Msa0242660:CDS 25.0%
!! AAAGTCTCAAACTTTAACTT+TGG + chr2_2:18970103-18970122 None:intergenic 25.0%
!! ATTTGATTCTGTTTTCTTGT+TGG - chr2_2:18970152-18970171 Msa0242660:CDS 25.0%
!! TTTGATTCTGTTTTCTTGTT+GGG - chr2_2:18970153-18970172 Msa0242660:CDS 25.0%
! CTTCTTAGAGAGAATAAAAA+CGG - chr2_2:18970225-18970244 Msa0242660:CDS 25.0%
! TTCTTAGAGAGAATAAAAAC+GGG - chr2_2:18970226-18970245 Msa0242660:CDS 25.0%
!!! TTCAAACTTTTGGAACATAT+TGG - chr2_2:18970294-18970313 Msa0242660:CDS 25.0%
! AAACAATGCTTACTAATAAC+AGG + chr2_2:18970336-18970355 None:intergenic 25.0%
! CAAGGACATAACATAATTAA+GGG + chr2_2:18970834-18970853 None:intergenic 25.0%
! GAAATATTAAGATTCTATGG+GGG + chr2_2:18970992-18971011 None:intergenic 25.0%
! GCAGAAATATTAAGATTCTA+TGG + chr2_2:18970995-18971014 None:intergenic 25.0%
! AAATTGATATTGATATGCAG+AGG - chr2_2:18971071-18971090 Msa0242660:intron 25.0%
! CTTAACAGTAACAAAAATAG+AGG + chr2_2:18971198-18971217 None:intergenic 25.0%
! AAGATCAATTTCATTTACAG+AGG + chr2_2:18971647-18971666 None:intergenic 25.0%
!! CTTTTGTTGACAATTCATTA+CGG - chr2_2:18972382-18972401 Msa0242660:intron 25.0%
!! TTTTGTTGACAATTCATTAC+GGG - chr2_2:18972383-18972402 Msa0242660:intron 25.0%
!! ATAATGAGTTTGACTTTGAT+GGG - chr2_2:18972617-18972636 Msa0242660:CDS 25.0%
TGCAAGTTACAACTGTTATA+AGG - chr2_2:18969475-18969494 Msa0242660:three_prime_UTR 30.0%
AAGATTGAAGACCTTCATAA+AGG + chr2_2:18969831-18969850 None:intergenic 30.0%
!! TATGGATGGATTCAAACTTT+TGG - chr2_2:18970284-18970303 Msa0242660:CDS 30.0%
!!! ACTTTTGGAACATATTGGAT+TGG - chr2_2:18970299-18970318 Msa0242660:CDS 30.0%
!!! TGAATGTGTTTTAGTGATGT+CGG - chr2_2:18970434-18970453 Msa0242660:CDS 30.0%
GCAAGGACATAACATAATTA+AGG + chr2_2:18970835-18970854 None:intergenic 30.0%
! TGAAAGAGAACATTTTTGCA+AGG + chr2_2:18970852-18970871 None:intergenic 30.0%
!! TCATCTTTGTTTGTTTTCCA+CGG - chr2_2:18970869-18970888 Msa0242660:intron 30.0%
! TTGGAAAAACTTTAAGGGAA+TGG + chr2_2:18970915-18970934 None:intergenic 30.0%
GAATCACAAGGATTATATCT+TGG + chr2_2:18970934-18970953 None:intergenic 30.0%
AACAATCTTGTAGAATCACA+AGG + chr2_2:18970946-18970965 None:intergenic 30.0%
GGTCATATCTCATATAACAT+GGG + chr2_2:18971224-18971243 None:intergenic 30.0%
AGGTCATATCTCATATAACA+TGG + chr2_2:18971225-18971244 None:intergenic 30.0%
! ATAAGTTAGTTTAGAAGTGG+TGG - chr2_2:18971248-18971267 Msa0242660:intron 30.0%
!! CGTACAAACTGATTTTGTAA+AGG - chr2_2:18971282-18971301 Msa0242660:intron 30.0%
! GATTTTGTAAAGGTTGAGTT+TGG - chr2_2:18971292-18971311 Msa0242660:intron 30.0%
ACTGTAACTCTTAACATTCA+AGG + chr2_2:18971463-18971482 None:intergenic 30.0%
!!! AATCTTAGTTTTGAGTTCTC+AGG + chr2_2:18971742-18971761 None:intergenic 30.0%
GAGAACTCAAAACTAAGATT+TGG - chr2_2:18971742-18971761 Msa0242660:CDS 30.0%
TCAAAACTAAGATTTGGAGA+TGG - chr2_2:18971748-18971767 Msa0242660:CDS 30.0%
CAAAACTAAGATTTGGAGAT+GGG - chr2_2:18971749-18971768 Msa0242660:CDS 30.0%
ATACAAATTCTCATGCTACA+AGG - chr2_2:18971980-18971999 Msa0242660:intron 30.0%
! CTATTGACTGATATTCCATT+TGG + chr2_2:18972036-18972055 None:intergenic 30.0%
ATATCCAGTAGCAGTATAAA+AGG - chr2_2:18972081-18972100 Msa0242660:CDS 30.0%
! TATAAAAGGTGCATTTCATG+CGG - chr2_2:18972095-18972114 Msa0242660:CDS 30.0%
ACATCAATGTTTCCTTGTAA+AGG + chr2_2:18972249-18972268 None:intergenic 30.0%
CGTAATGAATTGTCAACAAA+AGG + chr2_2:18972384-18972403 None:intergenic 30.0%
TTTGTTGACAATTCATTACG+GGG - chr2_2:18972384-18972403 Msa0242660:intron 30.0%
! AATATTTAGGGTCAACAGAA+AGG + chr2_2:18972511-18972530 None:intergenic 30.0%
! GATAATGAGTTTGACTTTGA+TGG - chr2_2:18972616-18972635 Msa0242660:CDS 30.0%
TAACAGGATCTGTTATAAAC+TGG + chr2_2:18972661-18972680 None:intergenic 30.0%
TAGTTGCCAATGTAAATAGT+TGG - chr2_2:18972697-18972716 Msa0242660:five_prime_UTR 30.0%
GAATGATGATTAGATTCATG+TGG + chr2_2:18972727-18972746 None:intergenic 30.0%
ATGTAAAAATTTCAGGCTTC+AGG - chr2_2:18972803-18972822 Msa0242660:five_prime_UTR 30.0%
TGTAAAAATTTCAGGCTTCA+GGG - chr2_2:18972804-18972823 Msa0242660:five_prime_UTR 30.0%
! GTTGTTGTTGTTGAGTTTCA+TGG + chr2_2:18969668-18969687 None:intergenic 35.0%
CATCATCAACAACAAGTACA+CGG + chr2_2:18969765-18969784 None:intergenic 35.0%
! AAAAACGGAGCATTCTTTCA+AGG + chr2_2:18969807-18969826 None:intergenic 35.0%
GACCTTCATAAAGGCAAAAA+CGG + chr2_2:18969822-18969841 None:intergenic 35.0%
AGTTTGAATCCATCCATATC+AGG + chr2_2:18970282-18970301 None:intergenic 35.0%
! GGAAAAAACGTGGTAATGAA+AGG - chr2_2:18970465-18970484 Msa0242660:CDS 35.0%
!! TCGAGGCTTTGAAGAATATA+TGG - chr2_2:18970532-18970551 Msa0242660:CDS 35.0%
TTCGGAAGAGAAAGAATAAC+TGG - chr2_2:18970565-18970584 Msa0242660:CDS 35.0%
AGATGAGTAATTAGCATCAG+TGG + chr2_2:18970646-18970665 None:intergenic 35.0%
TACTCATCTTCAACGAATGA+AGG - chr2_2:18970657-18970676 Msa0242660:CDS 35.0%
AAGAAGATGAAGCAGATGAT+AGG - chr2_2:18970706-18970725 Msa0242660:CDS 35.0%
AGAAGATGAAGCAGATGATA+GGG - chr2_2:18970707-18970726 Msa0242660:CDS 35.0%
TGAGCTTCATCAACAGTTTA+TGG - chr2_2:18970773-18970792 Msa0242660:CDS 35.0%
TATGGCTGTTGTGAATCAAT+TGG - chr2_2:18970791-18970810 Msa0242660:intron 35.0%
ATGGCTGTTGTGAATCAATT+GGG - chr2_2:18970792-18970811 Msa0242660:intron 35.0%
TAAGATTCTATGGGGGAAAA+GGG + chr2_2:18970985-18971004 None:intergenic 35.0%
TTAAGATTCTATGGGGGAAA+AGG + chr2_2:18970986-18971005 None:intergenic 35.0%
GGCTGTTCCTAAAAAGATTA+TGG - chr2_2:18971092-18971111 Msa0242660:intron 35.0%
ATGGAAATGATGAATGTTCC+TGG - chr2_2:18971111-18971130 Msa0242660:intron 35.0%
TGGAAATGATGAATGTTCCT+GGG - chr2_2:18971112-18971131 Msa0242660:intron 35.0%
ATATCATACCTGAAGATGAC+TGG + chr2_2:18971157-18971176 None:intergenic 35.0%
CAAAATCAGTTTGTACGATG+AGG + chr2_2:18971279-18971298 None:intergenic 35.0%
AGAGCCTATATAAGATCTCA+TGG - chr2_2:18971340-18971359 Msa0242660:intron 35.0%
GAGCCTATATAAGATCTCAT+GGG - chr2_2:18971341-18971360 Msa0242660:intron 35.0%
GTAACTCTTAACATTCAAGG+TGG + chr2_2:18971460-18971479 None:intergenic 35.0%
AATACCGTTTGTATCTTCGA+AGG - chr2_2:18971545-18971564 Msa0242660:CDS 35.0%
TCAACCTTCGAAGATACAAA+CGG + chr2_2:18971552-18971571 None:intergenic 35.0%
! ATTCAAGTTACTTTGCTGCT+GGG + chr2_2:18971584-18971603 None:intergenic 35.0%
! TATTCAAGTTACTTTGCTGC+TGG + chr2_2:18971585-18971604 None:intergenic 35.0%
AGTACGCAAGAGTCAATATT+TGG - chr2_2:18971616-18971635 Msa0242660:CDS 35.0%
TGAAAATTGGCAAACTGCTT+CGG + chr2_2:18971841-18971860 None:intergenic 35.0%
GTAGGTTGCATTAAAGATGA+TGG + chr2_2:18972009-18972028 None:intergenic 35.0%
TCATCTTTAATGCAACCTAC+AGG - chr2_2:18972009-18972028 Msa0242660:CDS 35.0%
ACTTCGGGAATTAAAGGATT+AGG - chr2_2:18972123-18972142 Msa0242660:CDS 35.0%
CTTCGGGAATTAAAGGATTA+GGG - chr2_2:18972124-18972143 Msa0242660:CDS 35.0%
TTCGGGAATTAAAGGATTAG+GGG - chr2_2:18972125-18972144 Msa0242660:CDS 35.0%
! ATTTGCTAATCCCCAATCAT+TGG + chr2_2:18972190-18972209 None:intergenic 35.0%
CAACATGCAAATCCTTTACA+AGG - chr2_2:18972234-18972253 Msa0242660:intron 35.0%
AATAGAGATGGTATGAGTCA+AGG - chr2_2:18972324-18972343 Msa0242660:intron 35.0%
! TGTCAACAAAAGGTGTAATG+AGG + chr2_2:18972374-18972393 None:intergenic 35.0%
TTAATCTCGATGTTTGTACC+TGG + chr2_2:18972426-18972445 None:intergenic 35.0%
TCTTGACTTTGATCAGAGAA+GGG + chr2_2:18972450-18972469 None:intergenic 35.0%
ATAAACTGGAATGTTGTCTG+CGG + chr2_2:18972647-18972666 None:intergenic 35.0%
TGATTAGAAAGAGAGCTAAC+AGG + chr2_2:18972677-18972696 None:intergenic 35.0%
GGAACACCAACTATTTACAT+TGG + chr2_2:18972706-18972725 None:intergenic 35.0%
TCAACTTCACCATCAAACTC+CGG - chr2_2:18969706-18969725 Msa0242660:CDS 40.0%
GCATTCTTTCAAGGATCATG+AGG + chr2_2:18969798-18969817 None:intergenic 40.0%
!! CTCCGTTTTTGCCTTTATGA+AGG - chr2_2:18969817-18969836 Msa0242660:intron 40.0%
! GGAACATATTGGATTGGAGA+TGG - chr2_2:18970305-18970324 Msa0242660:CDS 40.0%
! GTAATGAAAGGTGTGACACA+CGG - chr2_2:18970477-18970496 Msa0242660:CDS 40.0%
GAAGAATATATGGCAGCATG+TGG - chr2_2:18970542-18970561 Msa0242660:CDS 40.0%
ATATATGGCAGCATGTGGTT+CGG - chr2_2:18970547-18970566 Msa0242660:CDS 40.0%
! TGGAAAGATCTGAAGCAATC+TGG - chr2_2:18970585-18970604 Msa0242660:CDS 40.0%
! AAAGATCTGAAGCAATCTGG+TGG - chr2_2:18970588-18970607 Msa0242660:CDS 40.0%
TCAATAGACCAAACAACTCG+AGG + chr2_2:18970756-18970775 None:intergenic 40.0%
TGGCCCATGAGATCTTATAT+AGG + chr2_2:18971347-18971366 None:intergenic 40.0%
ATGTCCACTTATCAGTGTGA+GGG - chr2_2:18971413-18971432 Msa0242660:intron 40.0%
TTGTATCTTCGAAGGTTGAG+CGG - chr2_2:18971553-18971572 Msa0242660:CDS 40.0%
ACGCAAGAGTCAATATTTGG+AGG - chr2_2:18971619-18971638 Msa0242660:CDS 40.0%
ACATATGAGCAGCAGTAAAC+CGG - chr2_2:18971780-18971799 Msa0242660:CDS 40.0%
AAACCGGTGAACTTTCTTCA+CGG - chr2_2:18971796-18971815 Msa0242660:CDS 40.0%
ATTCCGTGAAGAAAGTTCAC+CGG + chr2_2:18971802-18971821 None:intergenic 40.0%
TTGAGCAGACTGGTGAAAAT+TGG + chr2_2:18971854-18971873 None:intergenic 40.0%
TCATTTAGCATCTGACCTCT+TGG + chr2_2:18971958-18971977 None:intergenic 40.0%
GATATTCCATTTGGTCCTGT+AGG + chr2_2:18972027-18972046 None:intergenic 40.0%
ATACTGCTACTGGATATACC+CGG + chr2_2:18972078-18972097 None:intergenic 40.0%
! TGCACCTTTTATACTGCTAC+TGG + chr2_2:18972088-18972107 None:intergenic 40.0%
ATTTCATGCGGAAGCTACTT+CGG - chr2_2:18972107-18972126 Msa0242660:CDS 40.0%
GAAGCTACTTCGGGAATTAA+AGG - chr2_2:18972117-18972136 Msa0242660:CDS 40.0%
TCGGGAATTAAAGGATTAGG+GGG - chr2_2:18972126-18972145 Msa0242660:CDS 40.0%
TCCATCAGAAGTCCAATGAT+TGG - chr2_2:18972175-18972194 Msa0242660:intron 40.0%
CCATCAGAAGTCCAATGATT+GGG - chr2_2:18972176-18972195 Msa0242660:intron 40.0%
CATCAGAAGTCCAATGATTG+GGG - chr2_2:18972177-18972196 Msa0242660:intron 40.0%
! GATTGGGGATTAGCAAATAC+AGG - chr2_2:18972192-18972211 Msa0242660:intron 40.0%
!! GTTGAGAAGCATCATATGTC+AGG + chr2_2:18972218-18972237 None:intergenic 40.0%
! CTCTATTTTGTCGAGTCTGT+TGG + chr2_2:18972311-18972330 None:intergenic 40.0%
CAGACTCGACAAAATAGAGA+TGG - chr2_2:18972312-18972331 Msa0242660:intron 40.0%
CAAGGTGATCACCAAAATGT+TGG - chr2_2:18972342-18972361 Msa0242660:intron 40.0%
!!! TGAGGTGTTGACCAACATTT+TGG + chr2_2:18972356-18972375 None:intergenic 40.0%
CTTGACTTTGATCAGAGAAG+GGG + chr2_2:18972449-18972468 None:intergenic 40.0%
CTCTTGACTTTGATCAGAGA+AGG + chr2_2:18972451-18972470 None:intergenic 40.0%
CTTCTCTGATCAAAGTCAAG+AGG - chr2_2:18972449-18972468 Msa0242660:intron 40.0%
TTGTATTTCTCGCAGCACAA+AGG - chr2_2:18972580-18972599 Msa0242660:CDS 40.0%
TGGTGAAGTTGAGGTTGACA+TGG + chr2_2:18969698-18969717 None:intergenic 45.0%
!! GGAGTTTGATGGTGAAGTTG+AGG + chr2_2:18969707-18969726 None:intergenic 45.0%
ATAAGGTTGCCGGAGTTTGA+TGG + chr2_2:18969718-18969737 None:intergenic 45.0%
CATCAACAACAAGTACACGG+AGG + chr2_2:18969762-18969781 None:intergenic 45.0%
CAAGGATCATGAGGCAAGTA+GGG + chr2_2:18969789-18969808 None:intergenic 45.0%
TCAAGGATCATGAGGCAAGT+AGG + chr2_2:18969790-18969809 None:intergenic 45.0%
TACCAAATGCAAGAGAGCTG+AGG - chr2_2:18970191-18970210 Msa0242660:CDS 45.0%
AACCTCAGCTCTCTTGCATT+TGG + chr2_2:18970196-18970215 None:intergenic 45.0%
GTGCATATGCCTGATATGGA+TGG - chr2_2:18970270-18970289 Msa0242660:CDS 45.0%
! TTAGTGATGTCGGCTGATGA+TGG - chr2_2:18970444-18970463 Msa0242660:CDS 45.0%
!! GGCTGATGATGGAAAAAACG+TGG - chr2_2:18970455-18970474 Msa0242660:CDS 45.0%
AATCAAACCTGTGCGAATCG+AGG - chr2_2:18970515-18970534 Msa0242660:CDS 45.0%
AAGGCAAGAGCTCAAAGAAG+AGG - chr2_2:18970676-18970695 Msa0242660:CDS 45.0%
CAAGAGCTCAAAGAAGAGGA+GGG - chr2_2:18970680-18970699 Msa0242660:CDS 45.0%
! CAAAGAAGCCTCGAGTTGTT+TGG - chr2_2:18970745-18970764 Msa0242660:CDS 45.0%
GTACGTTAGATGAGATACCG+TGG + chr2_2:18970889-18970908 None:intergenic 45.0%
AAACGTTGCCAGTCATCTTC+AGG - chr2_2:18971146-18971165 Msa0242660:intron 45.0%
TATAAGATCTCATGGGCCAC+TGG - chr2_2:18971348-18971367 Msa0242660:intron 45.0%
CACACTGATAAGTGGACATC+TGG + chr2_2:18971412-18971431 None:intergenic 45.0%
GATGTCCACTTATCAGTGTG+AGG - chr2_2:18971412-18971431 Msa0242660:intron 45.0%
TGTCCACTTATCAGTGTGAG+GGG - chr2_2:18971414-18971433 Msa0242660:intron 45.0%
TGACTCTTGCGTACTCATGA+AGG + chr2_2:18971611-18971630 None:intergenic 45.0%
CTTCAAACCCTTTCAGCTTC+TGG - chr2_2:18971664-18971683 Msa0242660:CDS 45.0%
GCTTCGGCTCAATGTTGATA+GGG + chr2_2:18971825-18971844 None:intergenic 45.0%
TGCTTCGGCTCAATGTTGAT+AGG + chr2_2:18971826-18971845 None:intergenic 45.0%
ATGCAACCTACAGGACCAAA+TGG - chr2_2:18972018-18972037 Msa0242660:CDS 45.0%
TTTCATGCGGAAGCTACTTC+GGG - chr2_2:18972108-18972127 Msa0242660:CDS 45.0%
CCCAATCATTGGACTTCTGA+TGG + chr2_2:18972179-18972198 None:intergenic 45.0%
!! GATGTCTTGCCATCGATTTC+AGG - chr2_2:18972264-18972283 Msa0242660:intron 45.0%
GGATAGAGGAAAAACCACGA+TGG + chr2_2:18972290-18972309 None:intergenic 45.0%
TTGTCGAGTCTGTTGGATAG+AGG + chr2_2:18972304-18972323 None:intergenic 45.0%
GGCAAGAGTGAAAGAATTCC+AGG - chr2_2:18972405-18972424 Msa0242660:intron 45.0%
! CATGAGTGTGTTTCTGAACC+AGG - chr2_2:18972476-18972495 Msa0242660:intron 45.0%
ACAGAAAGGACGAACAAACC+TGG + chr2_2:18972497-18972516 None:intergenic 45.0%
CCTTATCTCCGACCAGTTTC+CGG - chr2_2:18969732-18969751 Msa0242660:intron 50.0%
CGACGTGCATATGCCTGATA+TGG - chr2_2:18970266-18970285 Msa0242660:CDS 50.0%
! TTCAAAGCCTCGATTCGCAC+AGG + chr2_2:18970525-18970544 None:intergenic 50.0%
GCAAGAGCTCAAAGAAGAGG+AGG - chr2_2:18970679-18970698 Msa0242660:CDS 50.0%
! ACGTTTTCTCTAGTGAGCCC+AGG + chr2_2:18971132-18971151 None:intergenic 50.0%
GGCTATCAAGTCACCGCTAT+TGG - chr2_2:18971369-18971388 Msa0242660:intron 50.0%
GTCCACTTATCAGTGTGAGG+GGG - chr2_2:18971415-18971434 Msa0242660:intron 50.0%
GAACTGGCCAGAAGCTGAAA+GGG + chr2_2:18971674-18971693 None:intergenic 50.0%
AGAACTGGCCAGAAGCTGAA+AGG + chr2_2:18971675-18971694 None:intergenic 50.0%
TTCAGCTTCTGGCCAGTTCT+CGG - chr2_2:18971675-18971694 Msa0242660:CDS 50.0%
! AAGTCCTGCTGCTTGAAGCT+TGG + chr2_2:18971713-18971732 None:intergenic 50.0%
CACCAGTCTGCTCAATCCTT+TGG - chr2_2:18971859-18971878 Msa0242660:CDS 50.0%
TGCCAAAGGATTGAGCAGAC+TGG + chr2_2:18971864-18971883 None:intergenic 50.0%
CCTTTGGCAGCGCAAATATG+CGG - chr2_2:18971875-18971894 Msa0242660:CDS 50.0%
CTTTGGCAGCGCAAATATGC+GGG - chr2_2:18971876-18971895 Msa0242660:intron 50.0%
ATGGCTCAGTCTCAACCAAG+AGG - chr2_2:18971940-18971959 Msa0242660:intron 50.0%
TTTCCCTCTCAGATGCACTC+CGG - chr2_2:18972056-18972075 Msa0242660:CDS 50.0%
CTGGTCGGAGATAAGGTTGC+CGG + chr2_2:18969728-18969747 None:intergenic 55.0%
CCGGAAACTGGTCGGAGATA+AGG + chr2_2:18969735-18969754 None:intergenic 55.0%
TATCTCCGACCAGTTTCCGG+CGG - chr2_2:18969735-18969754 Msa0242660:intron 55.0%
! AGCGGTGACTTGATAGCCAG+TGG + chr2_2:18971367-18971386 None:intergenic 55.0%
CACCCCCTCACACTGATAAG+TGG + chr2_2:18971420-18971439 None:intergenic 55.0%
CTAGACTTTGCGCCGAGAAC+TGG + chr2_2:18971690-18971709 None:intergenic 55.0%
CTAGCCAAGCTTCAAGCAGC+AGG - chr2_2:18971706-18971725 Msa0242660:CDS 55.0%
CCGCATATTTGCGCTGCCAA+AGG + chr2_2:18971878-18971897 None:intergenic 55.0%
TGAGCCATCTGCGTCAACCA+GGG + chr2_2:18971928-18971947 None:intergenic 55.0%
TTCCCTCTCAGATGCACTCC+GGG - chr2_2:18972057-18972076 Msa0242660:CDS 55.0%
TACCCGGAGTGCATCTGAGA+GGG + chr2_2:18972062-18972081 None:intergenic 55.0%
ATACCCGGAGTGCATCTGAG+AGG + chr2_2:18972063-18972082 None:intergenic 55.0%
CCACGATGGCCTGAAATCGA+TGG + chr2_2:18972276-18972295 None:intergenic 55.0%
!! CCATCGATTTCAGGCCATCG+TGG - chr2_2:18972273-18972292 Msa0242660:intron 55.0%
! TTCTCGCAGCACAAAGGCGT+TGG - chr2_2:18972586-18972605 Msa0242660:CDS 55.0%
ATCTCCGACCAGTTTCCGGC+GGG - chr2_2:18969736-18969755 Msa0242660:intron 60.0%
! ATCTGGAGTGTGACTCGCGC+GGG + chr2_2:18971395-18971414 None:intergenic 60.0%
! CATCTGGAGTGTGACTCGCG+CGG + chr2_2:18971396-18971415 None:intergenic 60.0%
CTGCTGCAAAGAGAAGCCCC+TGG - chr2_2:18971908-18971927 Msa0242660:intron 60.0%
AAGCCCCTGGTTGACGCAGA+TGG - chr2_2:18971921-18971940 Msa0242660:intron 60.0%
GAGCCATCTGCGTCAACCAG+GGG + chr2_2:18971927-18971946 None:intergenic 60.0%
CTGAGCCATCTGCGTCAACC+AGG + chr2_2:18971929-18971948 None:intergenic 60.0%
ACAAGTACACGGAGGCCCGC+CGG + chr2_2:18969754-18969773 None:intergenic 65.0%
TGACTCGCGCGGGCCAATAG+CGG + chr2_2:18971385-18971404 None:intergenic 65.0%
GAGGCCCGCCGGAAACTGGT+CGG + chr2_2:18969743-18969762 None:intergenic 70.0%
CACGGAGGCCCGCCGGAAAC+TGG + chr2_2:18969747-18969766 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 18969462 18972880 18969462 ID=Msa0242660;Name=Msa0242660
chr2_2 mRNA 18969462 18972880 18969462 ID=Msa0242660-mRNA-1;Parent=Msa0242660;Name=Msa0242660-mRNA-1;_AED=0.03;_eAED=0.03;_QI=205|1|1|1|1|1|6|199|605
chr2_2 exon 18969462 18969747 18969462 ID=Msa0242660-mRNA-1:exon:7745;Parent=Msa0242660-mRNA-1
chr2_2 exon 18969845 18970798 18969845 ID=Msa0242660-mRNA-1:exon:7744;Parent=Msa0242660-mRNA-1
chr2_2 exon 18971175 18971251 18971175 ID=Msa0242660-mRNA-1:exon:7743;Parent=Msa0242660-mRNA-1
chr2_2 exon 18971520 18971894 18971520 ID=Msa0242660-mRNA-1:exon:7742;Parent=Msa0242660-mRNA-1
chr2_2 exon 18972000 18972152 18972000 ID=Msa0242660-mRNA-1:exon:7741;Parent=Msa0242660-mRNA-1
chr2_2 exon 18972504 18972880 18972504 ID=Msa0242660-mRNA-1:exon:7740;Parent=Msa0242660-mRNA-1
chr2_2 five_prime_UTR 18972676 18972880 18972676 ID=Msa0242660-mRNA-1:five_prime_utr;Parent=Msa0242660-mRNA-1
chr2_2 CDS 18972504 18972675 18972504 ID=Msa0242660-mRNA-1:cds;Parent=Msa0242660-mRNA-1
chr2_2 CDS 18972000 18972152 18972000 ID=Msa0242660-mRNA-1:cds;Parent=Msa0242660-mRNA-1
chr2_2 CDS 18971520 18971894 18971520 ID=Msa0242660-mRNA-1:cds;Parent=Msa0242660-mRNA-1
chr2_2 CDS 18971175 18971251 18971175 ID=Msa0242660-mRNA-1:cds;Parent=Msa0242660-mRNA-1
chr2_2 CDS 18969845 18970798 18969845 ID=Msa0242660-mRNA-1:cds;Parent=Msa0242660-mRNA-1
chr2_2 CDS 18969661 18969747 18969661 ID=Msa0242660-mRNA-1:cds;Parent=Msa0242660-mRNA-1
chr2_2 three_prime_UTR 18969462 18969660 18969462 ID=Msa0242660-mRNA-1:three_prime_utr;Parent=Msa0242660-mRNA-1
Gene Sequence

>Msa0242660

ATGAAACTCAACAACAACAACAAAATTTCCATGTCAACCTCAACTTCACCATCAAACTCCGGCAACCTTATCTCCGACCAGTTTCCGGCGGGCCTCCGTGTACTTGTTGTTGATGATGACCCTACTTGCCTCATGATCCTTGAAAGAATGCTCCGTTTTTGCCTTTATGAAGTTACCAAATGCAAGAGAGCTGAGGTTGCATTATCACTTCTTAGAGAGAATAAAAACGGGTTCGACATTGTTTTAAGCGACGTGCATATGCCTGATATGGATGGATTCAAACTTTTGGAACATATTGGATTGGAGATGGATCTTCCTGTTATTATGATGTCGGCTGATGATGGAAAAAACGTGGTAATGAAAGGTGTGACACACGGTGCTTGTGATTATCTAATCAAACCTGTGCGAATCGAGGCTTTGAAGAATATATGGCAGCATGTGGTTCGGAAGAGAAAGAATAACTGGAAAGATCTGAAGCAATCTGGTGGATTAGATGAAAGAGATCGAGAAAAAATTGTGTCCACTGATGCTAATTACTCATCTTCAACGAATGAAGGCAAGAGCTCAAAGAAGAGGAGGGATGAAGAAGATGAAGCAGATGATAGGGATGATTGTTCGACGTCAAAGAAGCCTCGAGTTGTTTGGTCTATTGAGCTTCATCAACAGTTTATGGCTGTTGTGAATCAATTGGGATTAGACAAGGCTGTTCCTAAAAAGATTATGGAAATGATGAATGTTCCTGGGCTCACTAGAGAAAACGTTGCCAGTCATCTTCAGAAATACCGTTTGTATCTTCGAAGGTTGAGCGGAGTTTCCCAGCAGCAAAGTAACTTGAATAACTCCTTCATGAGTACGCAAGAGTCAATATTTGGAGGAACCTCTGTAAATGAAATTGATCTTCAAACCCTTTCAGCTTCTGGCCAGTTCTCGGCGCAAAGTCTAGCCAAGCTTCAAGCAGCAGGACTTTACAGTCCTGAGAACTCAAAACTAAGATTTGGAGATGGGCAACTGCAACATATGAGCAGCAGTAAACCGGTGAACTTTCTTCACGGAATCCCTATCAACATTGAGCCGAAGCAGTTTGCCAATTTTCACCAGTCTGCTCAATCCTTTGGCAGCGCAAATATGCGGGTCAATGCTTCTGCTGCAAAGAGAAGCCCCTGGTTGACGCAGATGGCTCAGTCTCAACCAAGAGGTCAGATGCTAAATGAGAATACAAATTCTCATGCTACAAGGTTTCCATCATCTTTAATGCAACCTACAGGACCAAATGGAATATCAGTCAATAGTTTCCCTCTCAGATGCACTCCGGGTATATCCAGTAGCAGTATAAAAGGTGCATTTCATGCGGAAGCTACTTCGGGAATTAAAGGATTAGGGGGATTTAGTTATGATGTTTATAATGAACTCCATCAGAAGTCCAATGATTGGGGATTAGCAAATACAGGCCTGACATATGATGCTTCTCAACATGCAAATCCTTTACAAGGAAACATTGATGTCTTGCCATCGATTTCAGGCCATCGTGGTTTTTCCTCTATCCAACAGACTCGACAAAATAGAGATGGTATGAGTCAAGGTGATCACCAAAATGTTGGTCAACACCTCATTACACCTTTTGTTGACAATTCATTACGGGGCAAGAGTGAAAGAATTCCAGGTACAAACATCGAGATTAACCCCTTCTCTGATCAAAGTCAAGAGGATCTCATGAGTGTGTTTCTGAACCAGCACAAAGGCGTTGGACAAGTTGATAATGAGTTTGACTTTGATGGGTATTCCGCAGACAACATTCCAGTTTATAACAGATCCTGTTAG
Protein sequence

>Msa0242660

MKLNNNNKISMSTSTSPSNSGNLISDQFPAGLRVLVVDDDPTCLMILERMLRFCLYEVTKCKRAEVALSLLRENKNGFDIVLSDVHMPDMDGFKLLEHIGLEMDLPVIMMSADDGKNVVMKGVTHGACDYLIKPVRIEALKNIWQHVVRKRKNNWKDLKQSGGLDERDREKIVSTDANYSSSTNEGKSSKKRRDEEDEADDRDDCSTSKKPRVVWSIELHQQFMAVVNQLGLDKAVPKKIMEMMNVPGLTRENVASHLQKYRLYLRRLSGVSQQQSNLNNSFMSTQESIFGGTSVNEIDLQTLSASGQFSAQSLAKLQAAGLYSPENSKLRFGDGQLQHMSSSKPVNFLHGIPINIEPKQFANFHQSAQSFGSANMRVNASAAKRSPWLTQMAQSQPRGQMLNENTNSHATRFPSSLMQPTGPNGISVNSFPLRCTPGISSSSIKGAFHAEATSGIKGLGGFSYDVYNELHQKSNDWGLANTGLTYDASQHANPLQGNIDVLPSISGHRGFSSIQQTRQNRDGMSQGDHQNVGQHLITPFVDNSLRGKSERIPGTNIEINPFSDQSQEDLMSVFLNQHKGVGQVDNEFDFDGYSADNIPVYNRSC*