AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0243680


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0243680 MtrunA17_Chr2g0296861 90.506 158 15 0 1 158 1 158 6.57e-93 266
Msa0243680 MtrunA17_Chr3g0107461 50.000 154 76 1 1 153 1 154 1.34e-46 149
Msa0243680 MtrunA17_Chr3g0107381 45.570 158 86 0 1 158 1 158 8.11e-46 149
Msa0243680 MtrunA17_Chr3g0110131 44.156 154 86 0 1 154 1 154 6.57e-38 129
Msa0243680 MtrunA17_Chr4g0014321 37.662 154 95 1 1 153 1 154 4.63e-35 122
Msa0243680 MtrunA17_Chr3g0105791 47.541 122 63 1 30 150 2 123 5.12e-34 116
Msa0243680 MtrunA17_Chr4g0031421 46.400 125 66 1 30 153 2 126 1.49e-33 115
Msa0243680 MtrunA17_Chr3g0107331 40.268 149 89 0 4 152 3 151 2.06e-33 115
Msa0243680 MtrunA17_Chr2g0283941 38.125 160 94 2 1 157 1 158 1.70e-32 115
Msa0243680 MtrunA17_Chr4g0013041 35.849 159 99 2 1 158 1 157 9.63e-32 112
Msa0243680 MtrunA17_Chr5g0432691 33.987 153 101 0 1 153 1 153 3.77e-31 110
Msa0243680 MtrunA17_Chr4g0014031 37.179 156 97 1 1 155 1 156 1.29e-29 107
Msa0243680 MtrunA17_Chr3g0107361 37.162 148 93 0 6 153 5 152 1.31e-29 105
Msa0243680 MtrunA17_Chr8g0351751 37.975 158 95 2 1 155 1 158 1.60e-29 108
Msa0243680 MtrunA17_Chr3g0106421 43.077 130 73 1 30 158 2 131 3.44e-29 106
Msa0243680 MtrunA17_Chr1g0197461 33.987 153 100 1 6 158 5 156 5.14e-29 104
Msa0243680 MtrunA17_Chr3g0107351 35.811 148 95 0 6 153 5 152 1.08e-28 103
Msa0243680 MtrunA17_Chr5g0420351 34.839 155 100 1 1 155 1 154 1.30e-28 103
Msa0243680 MtrunA17_Chr4g0014121 37.580 157 97 1 1 156 29 185 1.10e-27 101
Msa0243680 MtrunA17_Chr1g0188681 34.641 153 99 1 1 153 1 152 1.30e-27 100
Msa0243680 MtrunA17_Chr3g0107341 36.111 144 92 0 6 149 5 148 6.82e-27 99.0
Msa0243680 MtrunA17_Chr1g0197261 31.613 155 105 1 1 155 1 154 3.26e-24 92.0
Msa0243680 MtrunA17_Chr2g0296831 36.752 117 74 0 1 117 1 117 1.68e-23 89.4
Msa0243680 MtrunA17_Chr4g0014131 35.032 157 101 1 1 156 1 157 6.18e-23 90.5
Msa0243680 MtrunA17_Chr1g0197321 31.210 157 104 3 1 155 1 155 6.28e-22 86.3
Msa0243680 MtrunA17_Chr1g0193311 34.839 155 100 1 1 155 1 154 7.36e-22 85.9
Msa0243680 MtrunA17_Chr3g0106501 38.583 127 77 1 30 155 2 128 8.26e-22 85.9
Msa0243680 MtrunA17_Chr4g0012761 35.256 156 98 3 1 155 1 154 1.51e-21 85.1
Msa0243680 MtrunA17_Chr7g0218151 32.258 155 104 1 1 155 1 154 4.31e-21 84.0
Msa0243680 MtrunA17_Chr1g0188661 30.323 155 107 1 1 155 1 154 2.75e-20 82.0
Msa0243680 MtrunA17_Chr1g0188651 34.839 155 94 4 1 153 1 150 8.67e-18 75.5
Msa0243680 MtrunA17_Chr4g0008701 28.814 118 80 1 11 128 14 127 4.18e-11 60.1
Msa0243680 MtrunA17_Chr7g0267601 27.273 121 78 2 11 128 14 127 5.33e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0243680 AT5G48670.1 41.558 154 90 0 1 154 1 154 4.49e-32 116
Msa0243680 AT3G05860.2 37.584 149 93 0 1 149 1 149 9.45e-30 108
Msa0243680 AT1G65300.1 37.748 151 94 0 4 154 3 153 9.74e-30 109
Msa0243680 AT1G65330.1 40.000 150 88 2 6 154 5 153 1.20e-29 109
Msa0243680 AT3G05860.3 39.744 156 80 3 1 149 1 149 5.18e-29 107
Msa0243680 AT3G05860.1 39.744 156 80 3 1 149 1 149 5.41e-29 107
Msa0243680 AT1G31630.1 33.333 153 91 3 6 153 5 151 4.89e-24 95.9
Msa0243680 AT1G31640.1 36.735 147 82 3 11 152 10 150 1.49e-23 95.9
Msa0243680 AT5G27810.1 43.182 88 50 0 30 117 2 89 7.04e-23 87.8
Msa0243680 AT5G26650.1 32.237 152 103 0 4 155 2 153 1.77e-22 92.0
Msa0243680 AT5G27960.1 31.579 152 104 0 4 155 2 153 1.51e-21 89.0
Msa0243680 AT1G22590.2 36.111 108 69 0 11 118 11 118 5.46e-21 84.3
Msa0243680 AT2G28700.1 29.530 149 105 0 1 149 1 149 5.40e-20 84.7
Msa0243680 AT2G40210.1 29.710 138 96 1 1 138 1 137 6.62e-15 71.2
Msa0243680 AT5G26580.1 28.931 159 89 5 1 154 1 140 3.12e-14 68.9
Msa0243680 AT5G26630.1 26.623 154 113 0 1 154 1 154 4.38e-14 67.4
Msa0243680 AT5G06500.1 28.182 110 75 1 1 110 1 106 3.93e-13 65.1

Find 30 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGATCTATCATTGCTTAT+TGG 0.201999 2_2:-20968211 Msa0243680:CDS
CTTCTCTTTCGATTTCATTA+TGG 0.221424 2_2:+20968339 None:intergenic
GGTGATTTCACTAATCTTCT+TGG 0.273592 2_2:+20968531 None:intergenic
ATTCATACGGGTGAATTTGA+TGG 0.333961 2_2:-20968256 Msa0243680:CDS
AGATGGTGTTGGACAAGTTT+AGG 0.350542 2_2:-20968423 Msa0243680:CDS
GTTTAGGAGCTTTCCTGAAT+CGG 0.417309 2_2:-20968407 Msa0243680:CDS
TAAACTTGTCCAACACCATC+TGG 0.418271 2_2:+20968425 None:intergenic
GGAAGACAACATTCAACAAA+AGG 0.464060 2_2:-20968953 Msa0243680:CDS
ATCTATGATGAAAACAATGA+TGG 0.489972 2_2:-20968478 Msa0243680:CDS
ACTTGGTGAATTCATTCATA+CGG 0.496749 2_2:-20968269 Msa0243680:CDS
AGAGAAGTTGAAGAAACAAA+AGG 0.500916 2_2:-20968323 Msa0243680:CDS
CCTGAATCGGAACAAAGAAA+AGG 0.516491 2_2:-20968394 Msa0243680:CDS
GGAACAAAGAAAAGGAAAGC+TGG 0.520124 2_2:-20968386 Msa0243680:CDS
AGTTGAAGAAACAAAAGGAC+AGG 0.532305 2_2:-20968318 Msa0243680:CDS
AAAAGGAAAGCTGGATCATG+AGG 0.532487 2_2:-20968377 Msa0243680:CDS
CTTGGTGAATTCATTCATAC+GGG 0.535817 2_2:-20968268 Msa0243680:CDS
AAGAAAATGAATGATCTACT+TGG 0.551967 2_2:-20968286 Msa0243680:CDS
ACGGTTTATCCATCAAAAGA+AGG 0.566324 2_2:-20968451 Msa0243680:CDS
ACATAACTGATGACTTGAAG+AGG 0.573872 2_2:-20968974 Msa0243680:CDS
AGAAGGAGTCCAGATGGTGT+TGG 0.590630 2_2:-20968434 Msa0243680:CDS
ACCGAAAAGAAGTCGATGAG+AGG 0.615793 2_2:-20968183 Msa0243680:CDS
ATCAAAAGAAGGAGTCCAGA+TGG 0.615817 2_2:-20968440 Msa0243680:CDS
TGAAAACAATGATGGAGCAA+CGG 0.627126 2_2:-20968470 Msa0243680:CDS
ACATTCAACAAAAGGAAGCA+TGG 0.630577 2_2:-20968945 Msa0243680:intron
AGTGAAATCACCACCCTGTG+TGG 0.639509 2_2:-20968520 Msa0243680:CDS
CACATGCATCAATTCCACAC+AGG 0.642340 2_2:+20968506 None:intergenic
ACATGCATCAATTCCACACA+GGG 0.652843 2_2:+20968507 None:intergenic
AAAGGAAAGCTGGATCATGA+GGG 0.661580 2_2:-20968376 Msa0243680:CDS
CTGTGTGGAATTGATGCATG+TGG 0.686522 2_2:-20968505 Msa0243680:CDS
TGCATCAATTCCACACAGGG+TGG 0.698056 2_2:+20968510 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAATAACAATAATTTCAA+AGG + chr2_2:20968513-20968532 None:intergenic 15.0%
!! GAAAATAACAATAATTTCAA+AGG + chr2_2:20968513-20968532 None:intergenic 15.0%
!! AGTGTTCTTTATAGAAATAA+AGG + chr2_2:20968262-20968281 None:intergenic 20.0%
!!! TTTCATATTTTTAGTATCCA+TGG + chr2_2:20968350-20968369 None:intergenic 20.0%
!! TCATATTGATAAATTCCTAA+TGG + chr2_2:20968386-20968405 None:intergenic 20.0%
!! ACATAACTGATATGTTATTA+TGG + chr2_2:20968487-20968506 None:intergenic 20.0%
!! AGTGTTCTTTATAGAAATAA+AGG + chr2_2:20968262-20968281 None:intergenic 20.0%
!!! TTTCATATTTTTAGTATCCA+TGG + chr2_2:20968350-20968369 None:intergenic 20.0%
!! TCATATTGATAAATTCCTAA+TGG + chr2_2:20968386-20968405 None:intergenic 20.0%
!! ACATAACTGATATGTTATTA+TGG + chr2_2:20968487-20968506 None:intergenic 20.0%
!!! GTTTTGTTTTGTTTTTCAAC+AGG - chr2_2:20968605-20968624 Msa0243680:intron 25.0%
! ATCTATGATGAAAACAATGA+TGG - chr2_2:20968686-20968705 Msa0243680:intron 25.0%
!! ATCATTCATTTTCTTCTTCT+TGG + chr2_2:20968873-20968892 None:intergenic 25.0%
! AAGAAAATGAATGATCTACT+TGG - chr2_2:20968878-20968897 Msa0243680:intron 25.0%
!!! GTTTTGTTTTGTTTTTCAAC+AGG - chr2_2:20968605-20968624 Msa0243680:intron 25.0%
! ATCTATGATGAAAACAATGA+TGG - chr2_2:20968686-20968705 Msa0243680:intron 25.0%
!! ATCATTCATTTTCTTCTTCT+TGG + chr2_2:20968873-20968892 None:intergenic 25.0%
! AAGAAAATGAATGATCTACT+TGG - chr2_2:20968878-20968897 Msa0243680:intron 25.0%
CTTCTCTTTCGATTTCATTA+TGG + chr2_2:20968828-20968847 None:intergenic 30.0%
AGAGAAGTTGAAGAAACAAA+AGG - chr2_2:20968841-20968860 Msa0243680:intron 30.0%
! ACTTGGTGAATTCATTCATA+CGG - chr2_2:20968895-20968914 Msa0243680:intron 30.0%
GAAGATCTATCATTGCTTAT+TGG - chr2_2:20968953-20968972 Msa0243680:CDS 30.0%
CTTCTCTTTCGATTTCATTA+TGG + chr2_2:20968828-20968847 None:intergenic 30.0%
AGAGAAGTTGAAGAAACAAA+AGG - chr2_2:20968841-20968860 Msa0243680:intron 30.0%
! ACTTGGTGAATTCATTCATA+CGG - chr2_2:20968895-20968914 Msa0243680:intron 30.0%
GAAGATCTATCATTGCTTAT+TGG - chr2_2:20968953-20968972 Msa0243680:CDS 30.0%
ACATAACTGATGACTTGAAG+AGG - chr2_2:20968190-20968209 Msa0243680:CDS 35.0%
GGAAGACAACATTCAACAAA+AGG - chr2_2:20968211-20968230 Msa0243680:CDS 35.0%
ACATTCAACAAAAGGAAGCA+TGG - chr2_2:20968219-20968238 Msa0243680:CDS 35.0%
AAGTTGACTGCATAACCATT+AGG - chr2_2:20968368-20968387 Msa0243680:CDS 35.0%
GGTGATTTCACTAATCTTCT+TGG + chr2_2:20968636-20968655 None:intergenic 35.0%
! TGAAAACAATGATGGAGCAA+CGG - chr2_2:20968694-20968713 Msa0243680:intron 35.0%
ACGGTTTATCCATCAAAAGA+AGG - chr2_2:20968713-20968732 Msa0243680:intron 35.0%
AGTTGAAGAAACAAAAGGAC+AGG - chr2_2:20968846-20968865 Msa0243680:intron 35.0%
CTTGGTGAATTCATTCATAC+GGG - chr2_2:20968896-20968915 Msa0243680:intron 35.0%
ATTCATACGGGTGAATTTGA+TGG - chr2_2:20968908-20968927 Msa0243680:intron 35.0%
ACATAACTGATGACTTGAAG+AGG - chr2_2:20968190-20968209 Msa0243680:CDS 35.0%
GGAAGACAACATTCAACAAA+AGG - chr2_2:20968211-20968230 Msa0243680:CDS 35.0%
ACATTCAACAAAAGGAAGCA+TGG - chr2_2:20968219-20968238 Msa0243680:CDS 35.0%
AAGTTGACTGCATAACCATT+AGG - chr2_2:20968368-20968387 Msa0243680:CDS 35.0%
GGTGATTTCACTAATCTTCT+TGG + chr2_2:20968636-20968655 None:intergenic 35.0%
! TGAAAACAATGATGGAGCAA+CGG - chr2_2:20968694-20968713 Msa0243680:intron 35.0%
ACGGTTTATCCATCAAAAGA+AGG - chr2_2:20968713-20968732 Msa0243680:intron 35.0%
AGTTGAAGAAACAAAAGGAC+AGG - chr2_2:20968846-20968865 Msa0243680:intron 35.0%
CTTGGTGAATTCATTCATAC+GGG - chr2_2:20968896-20968915 Msa0243680:intron 35.0%
ATTCATACGGGTGAATTTGA+TGG - chr2_2:20968908-20968927 Msa0243680:intron 35.0%
! CAGTTTTCTGATCACTACCA+TGG - chr2_2:20968330-20968349 Msa0243680:CDS 40.0%
CATTTCTCTCAACTCTCTCA+CGG - chr2_2:20968582-20968601 Msa0243680:intron 40.0%
ATTTCTCTCAACTCTCTCAC+GGG - chr2_2:20968583-20968602 Msa0243680:intron 40.0%
ACATGCATCAATTCCACACA+GGG + chr2_2:20968660-20968679 None:intergenic 40.0%
! ATCTGGACTCCTTCTTTTGA+TGG + chr2_2:20968725-20968744 None:intergenic 40.0%
ATCAAAAGAAGGAGTCCAGA+TGG - chr2_2:20968724-20968743 Msa0243680:intron 40.0%
TAAACTTGTCCAACACCATC+TGG + chr2_2:20968742-20968761 None:intergenic 40.0%
! AGATGGTGTTGGACAAGTTT+AGG - chr2_2:20968741-20968760 Msa0243680:intron 40.0%
GTTTAGGAGCTTTCCTGAAT+CGG - chr2_2:20968757-20968776 Msa0243680:intron 40.0%
! CCTTTTCTTTGTTCCGATTC+AGG + chr2_2:20968773-20968792 None:intergenic 40.0%
CCTGAATCGGAACAAAGAAA+AGG - chr2_2:20968770-20968789 Msa0243680:intron 40.0%
GGAACAAAGAAAAGGAAAGC+TGG - chr2_2:20968778-20968797 Msa0243680:intron 40.0%
AAAAGGAAAGCTGGATCATG+AGG - chr2_2:20968787-20968806 Msa0243680:intron 40.0%
AAAGGAAAGCTGGATCATGA+GGG - chr2_2:20968788-20968807 Msa0243680:intron 40.0%
!!! TCCTCTCATCGACTTCTTTT+CGG + chr2_2:20968985-20969004 None:intergenic 40.0%
! CAGTTTTCTGATCACTACCA+TGG - chr2_2:20968330-20968349 Msa0243680:CDS 40.0%
CATTTCTCTCAACTCTCTCA+CGG - chr2_2:20968582-20968601 Msa0243680:intron 40.0%
ATTTCTCTCAACTCTCTCAC+GGG - chr2_2:20968583-20968602 Msa0243680:intron 40.0%
ACATGCATCAATTCCACACA+GGG + chr2_2:20968660-20968679 None:intergenic 40.0%
! ATCTGGACTCCTTCTTTTGA+TGG + chr2_2:20968725-20968744 None:intergenic 40.0%
ATCAAAAGAAGGAGTCCAGA+TGG - chr2_2:20968724-20968743 Msa0243680:intron 40.0%
TAAACTTGTCCAACACCATC+TGG + chr2_2:20968742-20968761 None:intergenic 40.0%
! AGATGGTGTTGGACAAGTTT+AGG - chr2_2:20968741-20968760 Msa0243680:intron 40.0%
GTTTAGGAGCTTTCCTGAAT+CGG - chr2_2:20968757-20968776 Msa0243680:intron 40.0%
! CCTTTTCTTTGTTCCGATTC+AGG + chr2_2:20968773-20968792 None:intergenic 40.0%
CCTGAATCGGAACAAAGAAA+AGG - chr2_2:20968770-20968789 Msa0243680:intron 40.0%
GGAACAAAGAAAAGGAAAGC+TGG - chr2_2:20968778-20968797 Msa0243680:intron 40.0%
AAAAGGAAAGCTGGATCATG+AGG - chr2_2:20968787-20968806 Msa0243680:intron 40.0%
AAAGGAAAGCTGGATCATGA+GGG - chr2_2:20968788-20968807 Msa0243680:intron 40.0%
!!! TCCTCTCATCGACTTCTTTT+CGG + chr2_2:20968985-20969004 None:intergenic 40.0%
CACATGCATCAATTCCACAC+AGG + chr2_2:20968661-20968680 None:intergenic 45.0%
CTGTGTGGAATTGATGCATG+TGG - chr2_2:20968659-20968678 Msa0243680:intron 45.0%
ACCGAAAAGAAGTCGATGAG+AGG - chr2_2:20968981-20969000 Msa0243680:CDS 45.0%
CACATGCATCAATTCCACAC+AGG + chr2_2:20968661-20968680 None:intergenic 45.0%
CTGTGTGGAATTGATGCATG+TGG - chr2_2:20968659-20968678 Msa0243680:intron 45.0%
ACCGAAAAGAAGTCGATGAG+AGG - chr2_2:20968981-20969000 Msa0243680:CDS 45.0%
AGTGAAATCACCACCCTGTG+TGG - chr2_2:20968644-20968663 Msa0243680:intron 50.0%
TGCATCAATTCCACACAGGG+TGG + chr2_2:20968657-20968676 None:intergenic 50.0%
AGAAGGAGTCCAGATGGTGT+TGG - chr2_2:20968730-20968749 Msa0243680:intron 50.0%
AGTGAAATCACCACCCTGTG+TGG - chr2_2:20968644-20968663 Msa0243680:intron 50.0%
TGCATCAATTCCACACAGGG+TGG + chr2_2:20968657-20968676 None:intergenic 50.0%
AGAAGGAGTCCAGATGGTGT+TGG - chr2_2:20968730-20968749 Msa0243680:intron 50.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 20968162 20969024 20968162 ID=Msa0243680;Name=Msa0243680
chr2_2 mRNA 20968162 20969024 20968162 ID=Msa0243680-mRNA-1;Parent=Msa0243680;Name=Msa0243680-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|0|0|1|1|1|2|0|158
chr2_2 exon 20968946 20969024 20968946 ID=Msa0243680-mRNA-1:exon:8351;Parent=Msa0243680-mRNA-1
chr2_2 exon 20968162 20968559 20968162 ID=Msa0243680-mRNA-1:exon:8350;Parent=Msa0243680-mRNA-1
chr2_2 CDS 20968946 20969024 20968946 ID=Msa0243680-mRNA-1:cds;Parent=Msa0243680-mRNA-1
chr2_2 CDS 20968162 20968559 20968162 ID=Msa0243680-mRNA-1:cds;Parent=Msa0243680-mRNA-1
Gene Sequence

>Msa0243680

ATGGCTGATAAAAATATGAGAGTTGACTACATAACTGATGACTTGAAGAGGAAGACAACATTCAACAAAAGGAAGCATGGTATAACCAAGAAGATTAGTGAAATCACCACCCTGTGTGGAATTGATGCATGTGGTATAATCTATGATGAAAACAATGATGGAGCAACGGTTTATCCATCAAAAGAAGGAGTCCAGATGGTGTTGGACAAGTTTAGGAGCTTTCCTGAATCGGAACAAAGAAAAGGAAAGCTGGATCATGAGGGCTTTTTGAGACAATCCATAATGAAATCGAAAGAGAAGTTGAAGAAACAAAAGGACAGGTCCAAGAAGAAGAAAATGAATGATCTACTTGGTGAATTCATTCATACGGGTGAATTTGATGGAAATTTGAGCGAGAATGATCTCGAAGATCTATCATTGCTTATTGGTGATTACCGAAAAGAAGTCGATGAGAGGATCGAATCAAGTCAAACCTGA

Protein sequence

>Msa0243680

MADKNMRVDYITDDLKRKTTFNKRKHGITKKISEITTLCGIDACGIIYDENNDGATVYPSKEGVQMVLDKFRSFPESEQRKGKLDHEGFLRQSIMKSKEKLKKQKDRSKKKKMNDLLGEFIHTGEFDGNLSENDLEDLSLLIGDYRKEVDERIESSQT*