AlfalfaGEDB | Msa0245080

M. sativa cultivar zhongmu-4 / Msa0245080

KEGG: K09338
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00046.32

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0245080	XP_013463424.1	97.209	215	4	1	1	213	1	215	2.64e-147	418

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0245080	sp\|Q05466\|HAT4_ARATH	49.032	155	79	0	53	207	102	256	1.86e-42	147

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0245080	G7IIE0	97.209	215	4	1	1	213	1	215	1.26e-147	418

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TFs/TRs:

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Gene ID	Type	Classification
Msa0245080	TF	HB-other

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0245080	MtrunA17_Chr2g0299031	97.209	215	4	1	1	213	1	215	2.43e-151	418
Msa0245080	MtrunA17_Chr4g0055851	44.886	176	97	0	35	210	75	250	2.24e-42	144
Msa0245080	MtrunA17_Chr5g0400781	57.937	126	52	1	70	195	113	237	4.32e-41	140
Msa0245080	MtrunA17_Chr8g0335131	61.062	113	44	0	80	192	146	258	4.48e-41	141
Msa0245080	MtrunA17_Chr5g0399491	53.077	130	61	0	68	197	124	253	7.26e-41	140
Msa0245080	MtrunA17_Chr7g0258831	56.115	139	57	3	58	193	135	272	9.90e-41	140
Msa0245080	MtrunA17_Chr1g0175651	56.296	135	49	3	64	193	137	266	3.81e-40	139
Msa0245080	MtrunA17_Chr4g0054421	56.061	132	55	2	63	193	110	239	4.25e-40	138
Msa0245080	MtrunA17_Chr2g0308611	60.870	115	42	2	80	193	200	312	7.63e-38	134
Msa0245080	MtrunA17_Chr1g0151971	49.677	155	74	3	47	197	94	248	2.42e-36	129

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0245080	AT4G16780.1	49.032	155	79	0	53	207	102	256	1.89e-43	147
Msa0245080	AT4G17460.1	50.336	149	68	1	49	197	110	252	8.01e-42	143
Msa0245080	AT5G47370.1	57.627	118	50	0	80	197	130	247	3.69e-40	139
Msa0245080	AT3G60390.1	55.738	122	54	0	71	192	153	274	7.89e-40	139
Msa0245080	AT5G06710.1	56.452	124	53	1	70	193	180	302	1.23e-39	139
Msa0245080	AT4G37790.1	57.143	126	51	2	70	193	114	238	6.75e-39	135
Msa0245080	AT2G44910.1	57.895	114	48	0	80	193	163	276	1.26e-38	135
Msa0245080	AT2G22800.1	55.556	126	53	2	70	193	101	225	1.82e-37	131
Msa0245080	AT2G01430.1	54.386	114	49	1	80	193	139	249	4.79e-35	125
Msa0245080	AT1G70920.1	50.000	124	54	2	70	193	59	174	4.77e-29	108

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Find 36 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAATGTGAACAAACCTT+TGG	0.458446	2_2:+24003727	Msa0245080:CDS
AACTAAGTGAGGAGAATCTT+AGG	0.353207	2_2:+24004950	Msa0245080:CDS
AATAACAACATCCTCCTCAT+TGG	0.419038	2_2:-24005088	None:intergenic
AATATGGAAGCCCAATGAGG+AGG	0.651470	2_2:+24005077	Msa0245080:CDS
AATCATCTAAAGCTGCAAAT+TGG	0.315157	2_2:+24005031	Msa0245080:CDS
AATCTAACTCTATGGTGCTG+TGG	0.578396	2_2:-24005119	None:intergenic
AATTGTGCTGTGACGCTTGA+AGG	0.428719	2_2:-24003939	None:intergenic
ACACCACCAATGACAACAAC+GGG	0.443518	2_2:+24003864	Msa0245080:CDS
ACGGGTGCTCAAAGAAATTG+AGG	0.587563	2_2:+24003882	Msa0245080:CDS
AGCAAAGACAGGTTGAAGTT+TGG	0.373329	2_2:+24004214	Msa0245080:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACAATGTGAACAAACCTT+TGG	+	chr2_2:24003727-24003746	Msa0245080:CDS	30.0%
TTTCTTGCATGATGAACAAA+TGG	-	chr2_2:24005058-24005077	None:intergenic	30.0%
GTTCATCATGCAAGAAAATA+TGG	+	chr2_2:24005061-24005080	Msa0245080:CDS	30.0%
AAACAATGTGAACAAACCTT+TGG	+	chr2_2:24003727-24003746	Msa0245080:CDS	30.0%
TTTCTTGCATGATGAACAAA+TGG	-	chr2_2:24005058-24005077	None:intergenic	30.0%
GTTCATCATGCAAGAAAATA+TGG	+	chr2_2:24005061-24005080	Msa0245080:CDS	30.0%
CCTATCATGGTTCAAATCAA+TGG	-	chr2_2:24003774-24003793	None:intergenic	35.0%
TCAATTCATTTGCCCTATCA+TGG	-	chr2_2:24003787-24003806	None:intergenic	35.0%
TTTACATGCAGGCTCAAAAA+CGG	+	chr2_2:24004166-24004185	Msa0245080:intron	35.0%
AGAGCAAGGTATGAATCATT+TGG	+	chr2_2:24004249-24004268	Msa0245080:intron	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_2	gene	24003656	24005143	24003656	ID=Msa0245080;Name=Msa0245080
chr2_2	mRNA	24003656	24005143	24003656	ID=Msa0245080-mRNA-1;Parent=Msa0245080;Name=Msa0245080-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0\|0\|0\|1\|1\|1\|3\|0\|213
chr2_2	exon	24003656	24003967	24003656	ID=Msa0245080-mRNA-1:exon:9247;Parent=Msa0245080-mRNA-1
chr2_2	exon	24004177	24004256	24004177	ID=Msa0245080-mRNA-1:exon:9248;Parent=Msa0245080-mRNA-1
chr2_2	exon	24004894	24005143	24004894	ID=Msa0245080-mRNA-1:exon:9249;Parent=Msa0245080-mRNA-1
chr2_2	CDS	24003656	24003967	24003656	ID=Msa0245080-mRNA-1:cds;Parent=Msa0245080-mRNA-1
chr2_2	CDS	24004177	24004256	24004177	ID=Msa0245080-mRNA-1:cds;Parent=Msa0245080-mRNA-1
chr2_2	CDS	24004894	24005143	24004894	ID=Msa0245080-mRNA-1:cds;Parent=Msa0245080-mRNA-1

Gene Sequence

>Msa0245080

ATGGATGATGATCATCACATGGCATGCACGTTGGATCTTAATCTTAAACTTGGATTAGGAAGTTATGTGCAAAACAATGTGAACAAACCTTTGGTTGATTCAAAAGAAGAATTGGCCATTGATTTGAACCATGATAGGGCAAATGAATTGAGTTTGAAGAGAGTGCATGAAGAACAAGCAAACACAGTTGAAGAAGAAATTGCTATTGACACCACCAATGACAACAACGGGTGCTCAAAGAAATTGAGGCTTACAACTGAGCAATCAACCAAGCTTGAAAATGCCTTCAAGCGTCACAGCACAATTAACACCGCTCAAAAACGGGCACTTGCTGAGGAATTGAATTTAAAGCAAAGACAGGTTGAAGTTTGGTTCCAGAACAGAAGAGCAAGGACTAAGCTGAAACAAACAGAGGTGAACTGTATATACTTGAGAAAATGTCATGAGAAACTAAGTGAGGAGAATCTTAGGTTGAAGAAGGAATTAGAAGAACTGCGTGCATTAAAAGTTGGACCATCAAACACAACTCAATCATCTAAAGCTGCAAATTGGACCATTTGTTCATCATGCAAGAAAATATGGAAGCCCAATGAGGAGGATGTTGTTATTAAGAGCAGCCACAGCACCATAGAGTTAGATTAA

Protein sequence

>Msa0245080

MDDDHHMACTLDLNLKLGLGSYVQNNVNKPLVDSKEELAIDLNHDRANELSLKRVHEEQANTVEEEIAIDTTNDNNGCSKKLRLTTEQSTKLENAFKRHSTINTAQKRALAEELNLKQRQVEVWFQNRRARTKLKQTEVNCIYLRKCHEKLSEENLRLKKELEELRALKVGPSNTTQSSKAANWTICSSCKKIWKPNEEDVVIKSSHSTIELD*