AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0251000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0251000 MtrunA17_Chr2g0309101 88.551 428 30 2 42 451 1 427 0.0 733
Msa0251000 MtrunA17_Chr2g0320281 45.055 91 49 1 50 140 31 120 5.53e-21 95.5
Msa0251000 MtrunA17_Chr4g0030891 42.857 91 51 1 50 140 33 122 7.80e-21 94.4
Msa0251000 MtrunA17_Chr4g0028131 45.556 90 48 1 51 140 31 119 3.44e-20 93.2
Msa0251000 MtrunA17_Chr1g0212601 41.121 107 59 4 39 144 3 106 8.87e-18 82.8
Msa0251000 MtrunA17_Chr8g0382651 41.000 100 57 2 42 140 22 120 2.90e-17 83.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0251000 AT1G05577.1 39.791 382 167 13 46 422 7 330 1.79e-63 209
Msa0251000 AT2G28150.1 46.739 92 48 1 49 140 31 121 1.56e-23 103
Msa0251000 AT3G46110.2 43.269 104 58 1 37 140 5 107 2.37e-22 97.4
Msa0251000 AT3G46110.1 43.269 104 58 1 37 140 5 107 2.92e-22 97.8
Msa0251000 AT5G10150.1 39.823 113 66 2 42 154 36 146 5.25e-21 95.1
Msa0251000 AT5G59790.1 41.758 91 52 1 50 140 45 134 9.07e-21 94.7
Msa0251000 AT5G59790.2 41.758 91 52 1 50 140 45 134 1.14e-20 94.0
Msa0251000 AT5G10150.2 39.823 113 66 2 42 154 36 146 1.37e-20 93.6

Find 67 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGAGACAAAGATCTCTTT+TGG 0.288400 2_2:-40154698 Msa0251000:CDS
GGAAATCAGTCAGGATTCTT+TGG 0.308801 2_2:-40153122 Msa0251000:CDS
CAAATCCACACTACCCCTTT+TGG 0.310924 2_2:-40153495 Msa0251000:intron
ATATGTGTGGCAGGATTTAA+TGG 0.312486 2_2:-40153571 Msa0251000:CDS
GACGTATTCATTATCAGAAA+TGG 0.358743 2_2:+40153530 None:intergenic
TAGGACCAAGCAAAGGATTC+AGG 0.359159 2_2:+40153748 None:intergenic
CAAACTTCTTATAAACCATT+TGG 0.361784 2_2:-40152099 Msa0251000:CDS
GGCACATTAATAGGCTCCTT+AGG 0.392655 2_2:+40152704 None:intergenic
ACGTATTCATTATCAGAAAT+GGG 0.408507 2_2:+40153531 None:intergenic
TCAGTTACTTACGAGCAAAT+TGG 0.422889 2_2:+40152072 None:intergenic
GCACATTAATAGGCTCCTTA+GGG 0.443875 2_2:+40152705 None:intergenic
TGATCTAAATCAAATGTTAT+CGG 0.450570 2_2:-40153143 Msa0251000:CDS
CAAAAGGCGGAGAAGTTAGA+AGG 0.456209 2_2:-40154452 Msa0251000:CDS
TGTGGATTTGTGATCCTTTG+AGG 0.457496 2_2:+40153508 None:intergenic
ACTTCTTATAAACCATTTGG+TGG 0.460136 2_2:-40152096 Msa0251000:CDS
ATGATTCAACAACCTCTTGT+AGG 0.473719 2_2:+40153729 None:intergenic
AAAGATATTAAGAGATGGCT+TGG 0.473793 2_2:-40153793 Msa0251000:intron
GATTCTTCTTCTGATAAAGA+TGG 0.475364 2_2:+40153199 None:intergenic
GTTCTTCATCTTGCTCGAAA+CGG 0.488480 2_2:-40154366 Msa0251000:CDS
CATAATTTGATGAGTAAGAA+GGG 0.494107 2_2:-40152953 Msa0251000:CDS
TGATAAAGTACATACCAAAA+GGG 0.495189 2_2:+40153481 None:intergenic
CATGACCACTCATGTGACTA+AGG 0.495215 2_2:+40154412 None:intergenic
AATGTTATCGGAAATCAGTC+AGG 0.496121 2_2:-40153131 Msa0251000:CDS
GAGCAAATTGGTCCACCAAA+TGG 0.501260 2_2:+40152084 None:intergenic
TGCTCAGAAAACTGCCCCTA+AGG 0.506577 2_2:-40152720 Msa0251000:CDS
TCATAATTTGATGAGTAAGA+AGG 0.513194 2_2:-40152954 Msa0251000:CDS
CAAGAGTGGATATGTGTGGC+AGG 0.519703 2_2:-40153580 Msa0251000:CDS
TGTCATTGTTGAGTTTGTGT+TGG 0.520810 2_2:-40154531 Msa0251000:intron
AGATGGCTTGGTGAAGTGAG+AGG 0.523481 2_2:-40153781 Msa0251000:CDS
ACCTACCTGAATCCTTTGCT+TGG 0.529099 2_2:-40153753 Msa0251000:CDS
AAATCAAGCATGGAAACAAA+AGG 0.531171 2_2:-40154468 Msa0251000:CDS
AGAGATCTTTGTCTCTCTGC+CGG 0.533661 2_2:+40154703 None:intergenic
TTTCAGCTCGGACAGGTCAT+CGG 0.536641 2_2:-40153098 Msa0251000:CDS
GAGATGAAATAATCAAGAGC+AGG 0.544676 2_2:-40152146 Msa0251000:CDS
TAAGGAGCCTATTAATGTGC+CGG 0.548310 2_2:-40152702 Msa0251000:CDS
CTTGAAAAGATATTAAGAGA+TGG 0.548408 2_2:-40153798 Msa0251000:intron
ATCAAATTATGATACAAAGA+AGG 0.549252 2_2:+40152965 None:intergenic
AAATCTTGTAAAGGAATGAA+AGG 0.559264 2_2:-40151295 Msa0251000:CDS
TGTAAAGGAATGAAAGGCAT+GGG 0.564737 2_2:-40151289 Msa0251000:CDS
AAAAGGGCTGAGATTTGTAA+AGG 0.565816 2_2:-40152677 Msa0251000:CDS
AAACACATGAAATCTTGTAA+AGG 0.567959 2_2:-40151304 Msa0251000:CDS
TTGTAAAGGAATGAAAGGCA+TGG 0.572745 2_2:-40151290 Msa0251000:CDS
GATAATGAATACGTCCTCAA+AGG 0.574396 2_2:-40153522 Msa0251000:CDS
CGTATTCATTATCAGAAATG+GGG 0.578739 2_2:+40153532 None:intergenic
CCTTTGCTTGGTCCTACAAG+AGG 0.578853 2_2:-40153741 Msa0251000:intron
ATTTGTAAAGGAGACAAACT+AGG 0.580114 2_2:-40152665 Msa0251000:CDS
ACATACCAAAAGGGGTAGTG+TGG 0.583512 2_2:+40153490 None:intergenic
TGAAGCTGTACAAGTTGAAG+AGG 0.593606 2_2:-40153245 Msa0251000:CDS
GTTGATGATGCAGGCAATGT+GGG 0.595370 2_2:-40151354 Msa0251000:intron
AAGTTCATCAAAATCAAGCA+TGG 0.603008 2_2:-40154478 Msa0251000:CDS
CACATTAATAGGCTCCTTAG+GGG 0.608654 2_2:+40152706 None:intergenic
GATAAAGTACATACCAAAAG+GGG 0.611244 2_2:+40153482 None:intergenic
TACTTCCTTAGTCACATGAG+TGG 0.612205 2_2:-40154417 Msa0251000:CDS
ACAAACTAGGAGGATCAGCT+AGG 0.620815 2_2:-40152652 Msa0251000:intron
TTCAGCTACAACAATTGCTG+AGG 0.631029 2_2:-40151263 Msa0251000:CDS
TGAAGAACACGAATGAGATG+AGG 0.633710 2_2:+40154381 None:intergenic
AACTATAGGAGGTACAAGAG+TGG 0.640091 2_2:-40153594 Msa0251000:intron
GTATTCATTATCAGAAATGG+GGG 0.640428 2_2:+40153533 None:intergenic
TGAGACGATGTTGATGACGT+TGG 0.649599 2_2:+40152863 None:intergenic
TTGGATTGGTTGTGGAACTG+TGG 0.657832 2_2:-40152777 Msa0251000:CDS
CGAAACGGTGTTTATCTCAG+AGG 0.661351 2_2:-40154351 Msa0251000:intron
ACCAAGCAAAGGATTCAGGT+AGG 0.685603 2_2:+40153752 None:intergenic
GGTACAAGAGTGGATATGTG+TGG 0.687025 2_2:-40153584 Msa0251000:CDS
TGTAAAGGAGACAAACTAGG+AGG 0.689066 2_2:-40152662 Msa0251000:CDS
AGTTGATGATGCAGGCAATG+TGG 0.692418 2_2:-40151355 Msa0251000:intron
CCTCTTGTAGGACCAAGCAA+AGG 0.696251 2_2:+40153741 None:intergenic
TCAAGCATGGAAACAAAAGG+CGG 0.706704 2_2:-40154465 Msa0251000:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATGTGTATAAATAAAAAT+GGG + chr2_2:40152494-40152513 None:intergenic 10.0%
!! TTAATGTGTATAAATAAAAA+TGG + chr2_2:40152495-40152514 None:intergenic 10.0%
!! TATAATTATTTGAATCATAG+TGG - chr2_2:40151297-40151316 Msa0251000:CDS 15.0%
!! TAATTAAAACATTATTTGGT+TGG - chr2_2:40153709-40153728 Msa0251000:intron 15.0%
!! GAACAAATTGAATGAAAATA+TGG + chr2_2:40151628-40151647 None:intergenic 20.0%
!! CAAGGAAATAATGATTAATA+AGG + chr2_2:40152234-40152253 None:intergenic 20.0%
!! AGTTAAAGGTTAAAAAAATG+AGG + chr2_2:40152277-40152296 None:intergenic 20.0%
!! TTAGTTGCAAAAATTGATTA+TGG + chr2_2:40152312-40152331 None:intergenic 20.0%
!! CTAACAAATTATTGAACTAT+AGG - chr2_2:40152328-40152347 Msa0251000:intron 20.0%
!!! ACACATTAATATTTTGAGTA+TGG - chr2_2:40152506-40152525 Msa0251000:intron 20.0%
!! TTAGCAAAAGTTACATAATA+AGG + chr2_2:40152564-40152583 None:intergenic 20.0%
!!! TGATTTAGATCATTCTTTTT+TGG + chr2_2:40152785-40152804 None:intergenic 20.0%
!! TGATCTAAATCAAATGTTAT+CGG - chr2_2:40152793-40152812 Msa0251000:CDS 20.0%
!! ATCAAATTATGATACAAAGA+AGG + chr2_2:40152974-40152993 None:intergenic 20.0%
!! TTGAATAAACTTCAATTGTT+TGG - chr2_2:40153896-40153915 Msa0251000:intron 20.0%
!!! ATGAAAATTTTTGGTCTTAT+AGG - chr2_2:40153952-40153971 Msa0251000:intron 20.0%
! CTTGAAAAGATATTAAGAGA+TGG - chr2_2:40152138-40152157 Msa0251000:CDS 25.0%
! AAAAATGAGGTTGAGAAAAT+GGG + chr2_2:40152264-40152283 None:intergenic 25.0%
! AAAAAATGAGGTTGAGAAAA+TGG + chr2_2:40152265-40152284 None:intergenic 25.0%
! GGAGATACATATATAGTTAA+AGG + chr2_2:40152291-40152310 None:intergenic 25.0%
! ACAAATTATTGAACTATAGG+AGG - chr2_2:40152331-40152350 Msa0251000:intron 25.0%
! ACGTATTCATTATCAGAAAT+GGG + chr2_2:40152408-40152427 None:intergenic 25.0%
! TGATAAAGTACATACCAAAA+GGG + chr2_2:40152458-40152477 None:intergenic 25.0%
! ATGATAAAGTACATACCAAA+AGG + chr2_2:40152459-40152478 None:intergenic 25.0%
! TCATAATTTGATGAGTAAGA+AGG - chr2_2:40152982-40153001 Msa0251000:CDS 25.0%
! CATAATTTGATGAGTAAGAA+GGG - chr2_2:40152983-40153002 Msa0251000:CDS 25.0%
! CATGATCAAAATGAACATTA+TGG - chr2_2:40153331-40153350 Msa0251000:intron 25.0%
!! TTTGAAGAATGGTTTTTAAC+AGG + chr2_2:40153586-40153605 None:intergenic 25.0%
! TGTTAAAAACCATTCTTCAA+AGG - chr2_2:40153585-40153604 Msa0251000:CDS 25.0%
! GTTAAAAACCATTCTTCAAA+GGG - chr2_2:40153586-40153605 Msa0251000:CDS 25.0%
! CAAACTTCTTATAAACCATT+TGG - chr2_2:40153837-40153856 Msa0251000:intron 25.0%
! GATTTCATCTATGAAATGTT+AGG - chr2_2:40153918-40153937 Msa0251000:intron 25.0%
!! AAACTGTGCATGAAAATTTT+TGG - chr2_2:40153943-40153962 Msa0251000:intron 25.0%
!!! AAAATTTTTGGTCTTATAGG+TGG - chr2_2:40153955-40153974 Msa0251000:intron 25.0%
! CCATTTCTTCAAATTACTAA+TGG + chr2_2:40154071-40154090 None:intergenic 25.0%
! CCATTAGTAATTTGAAGAAA+TGG - chr2_2:40154068-40154087 Msa0251000:intron 25.0%
!!! TATAAGTTGATATTGATTGC+TGG - chr2_2:40154371-40154390 Msa0251000:CDS 25.0%
! TAATGTGTCTTTACACATAT+GGG + chr2_2:40154513-40154532 None:intergenic 25.0%
! TTAATGTGTCTTTACACATA+TGG + chr2_2:40154514-40154533 None:intergenic 25.0%
! AAACACATGAAATCTTGTAA+AGG - chr2_2:40154632-40154651 Msa0251000:intron 25.0%
! AAATCTTGTAAAGGAATGAA+AGG - chr2_2:40154641-40154660 Msa0251000:intron 25.0%
ACTAAGAGAAACAACTAACA+TGG + chr2_2:40151270-40151289 None:intergenic 30.0%
!! TCAAAAGAAGTGTCATTGTT+TGG - chr2_2:40151376-40151395 Msa0251000:intron 30.0%
AAGTTCATCAAAATCAAGCA+TGG - chr2_2:40151458-40151477 Msa0251000:intron 30.0%
AAATCAAGCATGGAAACAAA+AGG - chr2_2:40151468-40151487 Msa0251000:intron 30.0%
! TCATTCAATTTGTTCTCTAC+TGG - chr2_2:40151633-40151652 Msa0251000:intron 30.0%
TTGCACATAGTTCAAAATTG+CGG - chr2_2:40151724-40151743 Msa0251000:intron 30.0%
!! CCTCTAAAACCTTTATATTG+CGG + chr2_2:40151757-40151776 None:intergenic 30.0%
GTGTACTAAATTGTGTATCA+CGG + chr2_2:40151959-40151978 None:intergenic 30.0%
AATTGCAGATCATAGACAAT+AGG + chr2_2:40152025-40152044 None:intergenic 30.0%
AAAGATATTAAGAGATGGCT+TGG - chr2_2:40152143-40152162 Msa0251000:CDS 30.0%
GTATTCATTATCAGAAATGG+GGG + chr2_2:40152406-40152425 None:intergenic 30.0%
CGTATTCATTATCAGAAATG+GGG + chr2_2:40152407-40152426 None:intergenic 30.0%
GACGTATTCATTATCAGAAA+TGG + chr2_2:40152409-40152428 None:intergenic 30.0%
GATAAAGTACATACCAAAAG+GGG + chr2_2:40152457-40152476 None:intergenic 30.0%
GATTCTTCTTCTGATAAAGA+TGG + chr2_2:40152740-40152759 None:intergenic 30.0%
! TTGAAGAAGAGAAGCTTTTA+GGG - chr2_2:40152888-40152907 Msa0251000:CDS 30.0%
AGTAAGAAGGGTAAAAAAGA+TGG - chr2_2:40152995-40153014 Msa0251000:CDS 30.0%
! GCTTTTAATCTTAGTGAATG+AGG + chr2_2:40153107-40153126 None:intergenic 30.0%
TGACTAGTGTGTTCAAAAAT+TGG - chr2_2:40153140-40153159 Msa0251000:CDS 30.0%
AGTGTGTTCAAAAATTGGAT+TGG - chr2_2:40153145-40153164 Msa0251000:CDS 30.0%
!! TTCAAAAATTGGATTGGTTG+TGG - chr2_2:40153151-40153170 Msa0251000:CDS 30.0%
TATTAATGTGCCGGAAAAAA+GGG - chr2_2:40153243-40153262 Msa0251000:CDS 30.0%
ATTTGTAAAGGAGACAAACT+AGG - chr2_2:40153271-40153290 Msa0251000:CDS 30.0%
TTATGATACAATTTCTCACC+TGG + chr2_2:40153359-40153378 None:intergenic 30.0%
GATTCAAAACCATGTCTAAA+AGG + chr2_2:40153564-40153583 None:intergenic 30.0%
AATCCAATGCTACTATGATT+GGG - chr2_2:40153617-40153636 Msa0251000:intron 30.0%
! TTTTACAGAAAAAGCTGTGA+TGG - chr2_2:40153768-40153787 Msa0251000:CDS 30.0%
ACTTCTTATAAACCATTTGG+TGG - chr2_2:40153840-40153859 Msa0251000:intron 30.0%
GTAATTTGAAGAAATGGAAG+CGG - chr2_2:40154074-40154093 Msa0251000:intron 30.0%
GTATTGTCAATATCAGACAT+TGG - chr2_2:40154119-40154138 Msa0251000:intron 30.0%
AAATATACCGAAACTACAAC+TGG - chr2_2:40154242-40154261 Msa0251000:intron 30.0%
AATATACCGAAACTACAACT+GGG - chr2_2:40154243-40154262 Msa0251000:intron 30.0%
! AATTTTCCCAGTTGTAGTTT+CGG + chr2_2:40154252-40154271 None:intergenic 30.0%
GCATCATCAACTACTATTTA+TGG + chr2_2:40154573-40154592 None:intergenic 30.0%
!! TAAATAGTAGTTGATGATGC+AGG - chr2_2:40154573-40154592 Msa0251000:intron 30.0%
! AGAGAGACAAAGATCTCTTT+TGG - chr2_2:40151238-40151257 Msa0251000:CDS 35.0%
! GAGAGACAAAGATCTCTTTT+GGG - chr2_2:40151239-40151258 Msa0251000:CDS 35.0%
!!! GACACTTCTTTTGAAACACA+AGG + chr2_2:40151370-40151389 None:intergenic 35.0%
!! GAAGTGTCATTGTTTGGTTT+TGG - chr2_2:40151382-40151401 Msa0251000:intron 35.0%
TGTCATTGTTGAGTTTGTGT+TGG - chr2_2:40151405-40151424 Msa0251000:intron 35.0%
!! CCGCAATATAAAGGTTTTAG+AGG - chr2_2:40151754-40151773 Msa0251000:intron 35.0%
ACTATGCTGTCATAATTGCT+TGG - chr2_2:40151818-40151837 Msa0251000:intron 35.0%
ACACAATGTTGTCAAATAGC+TGG - chr2_2:40151975-40151994 Msa0251000:intron 35.0%
ATGATTCAACAACCTCTTGT+AGG + chr2_2:40152210-40152229 None:intergenic 35.0%
ATATGTGTGGCAGGATTTAA+TGG - chr2_2:40152365-40152384 Msa0251000:intron 35.0%
GATAATGAATACGTCCTCAA+AGG - chr2_2:40152414-40152433 Msa0251000:intron 35.0%
GAGTTTCTGCATTAGTACAA+AGG + chr2_2:40152628-40152647 None:intergenic 35.0%
AATGTTATCGGAAATCAGTC+AGG - chr2_2:40152805-40152824 Msa0251000:CDS 35.0%
! GTTGAAGAAGAGAAGCTTTT+AGG - chr2_2:40152887-40152906 Msa0251000:CDS 35.0%
!! CTTTTAGGGAATGGAAAAGA+AGG - chr2_2:40152902-40152921 Msa0251000:CDS 35.0%
GTAGAAAAAGTTGAATCAGG+AGG + chr2_2:40153052-40153071 None:intergenic 35.0%
GGAGTAGAAAAAGTTGAATC+AGG + chr2_2:40153055-40153074 None:intergenic 35.0%
!!! CTTTTTTCCGGCACATTAAT+AGG + chr2_2:40153244-40153263 None:intergenic 35.0%
CTATTAATGTGCCGGAAAAA+AGG - chr2_2:40153242-40153261 Msa0251000:CDS 35.0%
AAAAGGGCTGAGATTTGTAA+AGG - chr2_2:40153259-40153278 Msa0251000:CDS 35.0%
AAAATGAACATTATGGAGCC+AGG - chr2_2:40153338-40153357 Msa0251000:intron 35.0%
CAACTGCATACGCAATTTAA+AGG + chr2_2:40153400-40153419 None:intergenic 35.0%
TTAAATTGCGTATGCAGTTG+TGG - chr2_2:40153400-40153419 Msa0251000:intron 35.0%
ATTGCAATTGTATGTTGCAG+AGG - chr2_2:40153460-40153479 Msa0251000:intron 35.0%
! CCACAATACAAACCATTTTG+AGG + chr2_2:40153486-40153505 None:intergenic 35.0%
! CCTCAAAATGGTTTGTATTG+TGG - chr2_2:40153483-40153502 Msa0251000:intron 35.0%
GACTATGACTATTCTAAGCA+TGG + chr2_2:40153536-40153555 None:intergenic 35.0%
! AGTCATGATCCTTTTAGACA+TGG - chr2_2:40153552-40153571 Msa0251000:CDS 35.0%
GAATCCAATGCTACTATGAT+TGG - chr2_2:40153616-40153635 Msa0251000:intron 35.0%
TAACCCAATCATAGTAGCAT+TGG + chr2_2:40153623-40153642 None:intergenic 35.0%
GAGATGAAATAATCAAGAGC+AGG - chr2_2:40153790-40153809 Msa0251000:CDS 35.0%
TCAGTTACTTACGAGCAAAT+TGG + chr2_2:40153867-40153886 None:intergenic 35.0%
CGTGTCTATCAATGTTTGAT+AGG + chr2_2:40154025-40154044 None:intergenic 35.0%
!! CTAAAGTGTCGAAGTTACTT+AGG - chr2_2:40154177-40154196 Msa0251000:intron 35.0%
!! TTAGGTGTTGACTAGTTTGT+AGG - chr2_2:40154195-40154214 Msa0251000:intron 35.0%
!! TGTTGACTAGTTTGTAGGTT+GGG - chr2_2:40154200-40154219 Msa0251000:intron 35.0%
TCCAACAAAGAAAGCCAATA+AGG - chr2_2:40154273-40154292 Msa0251000:intron 35.0%
!! ACCTTATTGGCTTTCTTTGT+TGG + chr2_2:40154277-40154296 None:intergenic 35.0%
TTACGATGTTTACCAACTCT+TGG - chr2_2:40154395-40154414 Msa0251000:CDS 35.0%
! ATGTGTTTGTGCATTTTCTC+AGG + chr2_2:40154620-40154639 None:intergenic 35.0%
TTGTAAAGGAATGAAAGGCA+TGG - chr2_2:40154646-40154665 Msa0251000:intron 35.0%
! TGTAAAGGAATGAAAGGCAT+GGG - chr2_2:40154647-40154666 Msa0251000:intron 35.0%
TCAAGCATGGAAACAAAAGG+CGG - chr2_2:40151471-40151490 Msa0251000:intron 40.0%
TACTTCCTTAGTCACATGAG+TGG - chr2_2:40151519-40151538 Msa0251000:intron 40.0%
TGAAGAACACGAATGAGATG+AGG + chr2_2:40151558-40151577 None:intergenic 40.0%
GTTCTTCATCTTGCTCGAAA+CGG - chr2_2:40151570-40151589 Msa0251000:intron 40.0%
GGTACACAACCGCAATATAA+AGG - chr2_2:40151745-40151764 Msa0251000:intron 40.0%
!! ATTGCTTGGTTTGTCTGTTC+TGG - chr2_2:40151832-40151851 Msa0251000:intron 40.0%
GAGAAAATGGGACTAAAGCA+AGG + chr2_2:40152252-40152271 None:intergenic 40.0%
! AACTATAGGAGGTACAAGAG+TGG - chr2_2:40152342-40152361 Msa0251000:intron 40.0%
TATCAGAAATGGGGGTTATG+AGG + chr2_2:40152398-40152417 None:intergenic 40.0%
TGTGGATTTGTGATCCTTTG+AGG + chr2_2:40152431-40152450 None:intergenic 40.0%
! TGAAGCTGTACAAGTTGAAG+AGG - chr2_2:40152691-40152710 Msa0251000:CDS 40.0%
GGAAATCAGTCAGGATTCTT+TGG - chr2_2:40152814-40152833 Msa0251000:CDS 40.0%
!!! GGATTCTTTGGTTTTCAGCT+CGG - chr2_2:40152826-40152845 Msa0251000:CDS 40.0%
!! GAAGAGAAGCTTTTAGGGAA+TGG - chr2_2:40152893-40152912 Msa0251000:CDS 40.0%
CACATTAATAGGCTCCTTAG+GGG + chr2_2:40153233-40153252 None:intergenic 40.0%
GCACATTAATAGGCTCCTTA+GGG + chr2_2:40153234-40153253 None:intergenic 40.0%
TAAGGAGCCTATTAATGTGC+CGG - chr2_2:40153234-40153253 Msa0251000:CDS 40.0%
!!! CAAATCTCAGCCCTTTTTTC+CGG + chr2_2:40153256-40153275 None:intergenic 40.0%
TGTAAAGGAGACAAACTAGG+AGG - chr2_2:40153274-40153293 Msa0251000:CDS 40.0%
TTACACTGCGAACCAAAATG+TGG - chr2_2:40153423-40153442 Msa0251000:intron 40.0%
TTGTATTGTGGCCACAATTG+CGG - chr2_2:40153495-40153514 Msa0251000:intron 40.0%
! CACAAACACCCTTTGAAGAA+TGG + chr2_2:40153597-40153616 None:intergenic 40.0%
!! GTGTTGACTAGTTTGTAGGT+TGG - chr2_2:40154199-40154218 Msa0251000:intron 40.0%
CAGCAGAATCTTGACCTTAT+TGG + chr2_2:40154290-40154309 None:intergenic 40.0%
CAATAAGGTCAAGATTCTGC+TGG - chr2_2:40154288-40154307 Msa0251000:intron 40.0%
TTCAGCTACAACAATTGCTG+AGG - chr2_2:40154673-40154692 Msa0251000:intron 40.0%
! CAGTTCTTTTAGCAGCATGC+CGG - chr2_2:40151214-40151233 Msa0251000:CDS 45.0%
AGAGATCTTTGTCTCTCTGC+CGG + chr2_2:40151236-40151255 None:intergenic 45.0%
CAAAAGGCGGAGAAGTTAGA+AGG - chr2_2:40151484-40151503 Msa0251000:intron 45.0%
CATGACCACTCATGTGACTA+AGG + chr2_2:40151527-40151546 None:intergenic 45.0%
CGAAACGGTGTTTATCTCAG+AGG - chr2_2:40151585-40151604 Msa0251000:intron 45.0%
!! TTCTCTACTGGCTCTGATCT+AGG - chr2_2:40151645-40151664 Msa0251000:intron 45.0%
ACCTACCTGAATCCTTTGCT+TGG - chr2_2:40152183-40152202 Msa0251000:intron 45.0%
ACCAAGCAAAGGATTCAGGT+AGG + chr2_2:40152187-40152206 None:intergenic 45.0%
TAGGACCAAGCAAAGGATTC+AGG + chr2_2:40152191-40152210 None:intergenic 45.0%
GGTACAAGAGTGGATATGTG+TGG - chr2_2:40152352-40152371 Msa0251000:intron 45.0%
! CAAATCCACACTACCCCTTT+TGG - chr2_2:40152441-40152460 Msa0251000:intron 45.0%
ACATACCAAAAGGGGTAGTG+TGG + chr2_2:40152449-40152468 None:intergenic 45.0%
!! TGAGACGATGTTGATGACGT+TGG + chr2_2:40153076-40153095 None:intergenic 45.0%
!! TTGGATTGGTTGTGGAACTG+TGG - chr2_2:40153159-40153178 Msa0251000:CDS 45.0%
GGCACATTAATAGGCTCCTT+AGG + chr2_2:40153235-40153254 None:intergenic 45.0%
ACAAACTAGGAGGATCAGCT+AGG - chr2_2:40153284-40153303 Msa0251000:CDS 45.0%
ATGTTGCAGAGGCCTCAAAA+TGG - chr2_2:40153471-40153490 Msa0251000:intron 45.0%
AAGTTGCACAACCGCAATTG+TGG + chr2_2:40153509-40153528 None:intergenic 45.0%
GAGCAAATTGGTCCACCAAA+TGG + chr2_2:40153855-40153874 None:intergenic 45.0%
CGAAACCCATACTTACACGT+AGG - chr2_2:40154043-40154062 Msa0251000:intron 45.0%
TGGTGCCTACGTGTAAGTAT+GGG + chr2_2:40154051-40154070 None:intergenic 45.0%
ATGGTGCCTACGTGTAAGTA+TGG + chr2_2:40154052-40154071 None:intergenic 45.0%
TTTACCAACTCTTGGCTGCA+TGG - chr2_2:40154403-40154422 Msa0251000:CDS 45.0%
TTACCAACTCTTGGCTGCAT+GGG - chr2_2:40154404-40154423 Msa0251000:CDS 45.0%
!! AGTTGATGATGCAGGCAATG+TGG - chr2_2:40154581-40154600 Msa0251000:intron 45.0%
!! GTTGATGATGCAGGCAATGT+GGG - chr2_2:40154582-40154601 Msa0251000:intron 45.0%
!! ATATATATATAATTAACAAA+AGG - chr2_2:40151327-40151346 Msa0251000:CDS 5.0%
!! TATATATATAATTAACAAAA+GGG - chr2_2:40151328-40151347 Msa0251000:CDS 5.0%
!!! AAATAATGTTTTAATTAAAT+TGG + chr2_2:40153706-40153725 None:intergenic 5.0%
!! AATTTAATTAAAACATTATT+TGG - chr2_2:40153705-40153724 Msa0251000:intron 5.0%
!! AGATGGCTTGGTGAAGTGAG+AGG - chr2_2:40152155-40152174 Msa0251000:CDS 50.0%
CCTCTTGTAGGACCAAGCAA+AGG + chr2_2:40152198-40152217 None:intergenic 50.0%
CCTTTGCTTGGTCCTACAAG+AGG - chr2_2:40152195-40152214 Msa0251000:intron 50.0%
CAAGAGTGGATATGTGTGGC+AGG - chr2_2:40152356-40152375 Msa0251000:intron 50.0%
!!! CTTTGGTTTTCAGCTCGGAC+AGG - chr2_2:40152831-40152850 Msa0251000:CDS 50.0%
TTTCAGCTCGGACAGGTCAT+CGG - chr2_2:40152838-40152857 Msa0251000:CDS 50.0%
! TAGGGGCAGTTTTCTGAGCA+GGG + chr2_2:40153216-40153235 None:intergenic 50.0%
! TTAGGGGCAGTTTTCTGAGC+AGG + chr2_2:40153217-40153236 None:intergenic 50.0%
TGCTCAGAAAACTGCCCCTA+AGG - chr2_2:40153216-40153235 Msa0251000:CDS 50.0%
GAGGATCAGCTAGGTGCTTT+GGG - chr2_2:40153293-40153312 Msa0251000:CDS 50.0%
!! CTAGGTGCTTTGGGACTTCT+TGG - chr2_2:40153302-40153321 Msa0251000:intron 50.0%
! CGACTGCATCAGCCACATTT+TGG + chr2_2:40153438-40153457 None:intergenic 50.0%
AGGTCAAGATTCTGCTGGTG+TGG - chr2_2:40154293-40154312 Msa0251000:intron 50.0%
GGAGGATCAGCTAGGTGCTT+TGG - chr2_2:40153292-40153311 Msa0251000:CDS 55.0%
TAGCCCATGCAGCCAAGAGT+TGG + chr2_2:40154410-40154429 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 40151207 40154751 40151207 ID=Msa0251000;Name=Msa0251000
chr2_2 mRNA 40151207 40154751 40151207 ID=Msa0251000-mRNA-1;Parent=Msa0251000;Name=Msa0251000-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|0|0|1|0.83|0.85|7|0|451
chr2_2 exon 40154688 40154751 40154688 ID=Msa0251000-mRNA-1:exon:12491;Parent=Msa0251000-mRNA-1
chr2_2 exon 40154352 40154540 40154352 ID=Msa0251000-mRNA-1:exon:12490;Parent=Msa0251000-mRNA-1
chr2_2 exon 40153742 40153811 40153742 ID=Msa0251000-mRNA-1:exon:12489;Parent=Msa0251000-mRNA-1
chr2_2 exon 40153496 40153608 40153496 ID=Msa0251000-mRNA-1:exon:12488;Parent=Msa0251000-mRNA-1
chr2_2 exon 40152653 40153316 40152653 ID=Msa0251000-mRNA-1:exon:12487;Parent=Msa0251000-mRNA-1
chr2_2 exon 40152084 40152182 40152084 ID=Msa0251000-mRNA-1:exon:12486;Parent=Msa0251000-mRNA-1
chr2_2 exon 40151207 40151363 40151207 ID=Msa0251000-mRNA-1:exon:12485;Parent=Msa0251000-mRNA-1
chr2_2 CDS 40154688 40154751 40154688 ID=Msa0251000-mRNA-1:cds;Parent=Msa0251000-mRNA-1
chr2_2 CDS 40154352 40154540 40154352 ID=Msa0251000-mRNA-1:cds;Parent=Msa0251000-mRNA-1
chr2_2 CDS 40153742 40153811 40153742 ID=Msa0251000-mRNA-1:cds;Parent=Msa0251000-mRNA-1
chr2_2 CDS 40153496 40153608 40153496 ID=Msa0251000-mRNA-1:cds;Parent=Msa0251000-mRNA-1
chr2_2 CDS 40152653 40153316 40152653 ID=Msa0251000-mRNA-1:cds;Parent=Msa0251000-mRNA-1
chr2_2 CDS 40152084 40152182 40152084 ID=Msa0251000-mRNA-1:cds;Parent=Msa0251000-mRNA-1
chr2_2 CDS 40151207 40151363 40151207 ID=Msa0251000-mRNA-1:cds;Parent=Msa0251000-mRNA-1
Gene Sequence

>Msa0251000

TTGGTAACAGTTCTTTTAGCAGCATGCCGGCAGAGAGACAAAGATCTCTTTTGGGTTTTTCCATTTTGTGTTGGTGTTTATTGCAGCAAAGATAACAACAAAACAAGTTCATCAAAATCAAGCATGGAAACAAAAGGCGGAGAAGTTAGAAGGCTTCACATAATTTACTTCCTTAGTCACATGAGTGGTCATGCAGAACATCCTCATCTCATTCGTGTTCTTCATCTTGCTCGAAACGGTGTTTATCTCAGAGATATTAAGAGATGGCTTGGTGAAGTGAGAGGAAGAGACCTACCTGAATCCTTTGCTTGGTCCTACAAGAGGAGGTACAAGAGTGGATATGTGTGGCAGGATTTAATGGATGACGACCTCATAACCCCCATTTCTGATAATGAATACGTCCTCAAAGGATCACAAATCCACACTACCCCTTTTGAAACTCATGCAATTAATTCAACAATCAATGAAAAGAAAACTGATACTGATGAAGCTGTACAAGTTGAAGAGGACAAACAAAAGCAGCAAAAGAAACCATCTTTATCAGAAGAAGAATCTGAAATTCAGAAAGAAACTGATCCAAAAAAGAATGATCTAAATCAAATGTTATCGGAAATCAGTCAGGATTCTTTGGTTTTCAGCTCGGACAGGTCATCGGTGACTGACGATGATTCTTCTTTTAAAGTTGAAGAAGAGAAGCTTTTAGGGAATGGAAAAGAAGGTTGCTTGAATGACAAGAATCATGAGAAGCTTGAGAGTTTCTCATTTCCTTCTTTGTATCATAATTTGATGAGTAAGAAGGGTAAAAAAGATGGTCACAATAACAAAACTGATACTACTACTACACCTCCTGATTCAACTTTTTCTACTCCAACGTCATCAACATCGTCTCAATCTCAATCCTCATTCACTAAGATTAAAAGCAATTCTATAAGAGTGACTAGTGTGTTCAAAAATTGGATTGGTTGTGGAACTGTGGCAACAAATGATGCTGCTTTTGTCTCTATGAACCCTGCTCAGAAAACTGCCCCTAAGGAGCCTATTAATGTGCCGGAAAAAAGGGCTGAGATTTGTAAAGGAGACAAACTAGGAGGATCAGCTAGAAAAAGCTGTGATGGAGATGAAATAATCAAGAGCAGGAAAAAGCTAAGCGAATTATTGAGTCAAACTTCTTATAAACCATTTGGTGGACCAATTTGCTCGCAATGTGGGAAGTCATTCAAGCCTGAGAAAATGCACAAACACATGAAATCTTGTAAAGGAATGAAAGGCATGGGAACTTCAGCTACAACAATTGCTGAGGAAACACAGCTTCATAGATCAACTTCATCATCTAAAGATTACCTTTTGAAAAATTAA
Protein sequence

>Msa0251000

LVTVLLAACRQRDKDLFWVFPFCVGVYCSKDNNKTSSSKSSMETKGGEVRRLHIIYFLSHMSGHAEHPHLIRVLHLARNGVYLRDIKRWLGEVRGRDLPESFAWSYKRRYKSGYVWQDLMDDDLITPISDNEYVLKGSQIHTTPFETHAINSTINEKKTDTDEAVQVEEDKQKQQKKPSLSEEESEIQKETDPKKNDLNQMLSEISQDSLVFSSDRSSVTDDDSSFKVEEEKLLGNGKEGCLNDKNHEKLESFSFPSLYHNLMSKKGKKDGHNNKTDTTTTPPDSTFSTPTSSTSSQSQSSFTKIKSNSIRVTSVFKNWIGCGTVATNDAAFVSMNPAQKTAPKEPINVPEKRAEICKGDKLGGSARKSCDGDEIIKSRKKLSELLSQTSYKPFGGPICSQCGKSFKPEKMHKHMKSCKGMKGMGTSATTIAEETQLHRSTSSSKDYLLKN*