AlfalfaGEDB | Msa0251530

M. sativa cultivar zhongmu-4 / Msa0251530

KEGG: -
GO: GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006355 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009642 GO:0009644 GO:0009889 GO:0009891 GO:0009893 GO:0009962 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141
PFAM: PF02365.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0251530	XP_003595952.1	94.265	279	16	0	1	279	1	279	0.0	554
Msa0251530	XP_003595952.1	95.971	273	11	0	276	548	1	273	0.0	553

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0251530	sp\|A4VCM0\|NAC45_ARATH	73.585	159	42	0	1	159	1	159	7.08e-83	269
Msa0251530	sp\|A4VCM0\|NAC45_ARATH	72.956	159	43	0	276	434	1	159	2.33e-82	268

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0251530	G7IGW3	94.265	279	16	0	1	279	1	279	0.0	554
Msa0251530	G7IGW3	95.971	273	11	0	276	548	1	273	0.0	553

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TFs/TRs:

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Gene ID	Type	Classification
Msa0251530	TF	NAC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0251530	MtrunA17_Chr2g0309891	94.265	279	16	0	1	279	1	279	0.0	554
Msa0251530	MtrunA17_Chr2g0309891	95.971	273	11	0	276	548	1	273	0.0	553
Msa0251530	MtrunA17_Chr3g0109821	74.405	168	43	0	1	168	1	168	4.29e-85	277
Msa0251530	MtrunA17_Chr3g0109821	73.810	168	44	0	276	443	1	168	1.53e-84	276
Msa0251530	MtrunA17_Chr5g0433781	67.368	190	60	2	1	189	1	189	8.36e-84	273
Msa0251530	MtrunA17_Chr5g0433781	66.842	190	61	2	276	464	1	189	2.74e-83	272
Msa0251530	MtrunA17_Chr6g0481091	72.611	157	42	1	1	157	1	156	4.54e-81	253
Msa0251530	MtrunA17_Chr6g0481091	71.975	157	43	1	276	432	1	156	1.46e-80	252
Msa0251530	MtrunA17_Chr5g0400461	50.711	211	85	3	276	479	1	199	2.76e-65	216
Msa0251530	MtrunA17_Chr5g0400461	50.711	211	85	3	1	204	1	199	3.16e-65	215

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0251530	AT1G54330.1	78.049	164	32	1	276	439	1	160	5.11e-87	271
Msa0251530	AT1G54330.1	78.049	164	32	1	1	164	1	160	5.81e-87	271
Msa0251530	AT3G17730.1	77.707	157	34	1	1	157	1	156	5.20e-85	264
Msa0251530	AT3G17730.1	77.070	157	35	1	276	432	1	156	1.66e-84	263
Msa0251530	AT3G03200.1	73.585	159	42	0	1	159	1	159	7.20e-84	269
Msa0251530	AT3G03200.1	72.956	159	43	0	276	434	1	159	2.37e-83	268
Msa0251530	AT1G65910.1	71.023	176	47	2	1	176	1	172	3.82e-83	271
Msa0251530	AT1G65910.1	70.455	176	48	2	276	451	1	172	1.28e-82	270
Msa0251530	AT5G17260.1	72.956	159	43	0	1	159	1	159	4.74e-83	267
Msa0251530	AT5G17260.1	72.327	159	44	0	276	434	1	159	1.59e-82	265

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Find 122 sgRNAs with CRISPR-Local

Find 465 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAAGAGAGAGAAGCACC+AGG	0.628486	2_2:+41260886	None:intergenic
AAAGAAGAGAGAGAAGCACC+AGG	0.628486	2_2:+41268332	None:intergenic
AACAGGAAGAACTATAGAGC+TGG	0.515756	2_2:-41261328	Msa0251530:CDS
AACAGGAAGAACTATAGAGC+TGG	0.515756	2_2:-41268774	Msa0251530:CDS
AACTCTCAAAAGAAACAAGT+TGG	0.494236	2_2:-41261003	Msa0251530:CDS
AACTCTCAAAAGAGACAAGT+TGG	0.466190	2_2:-41268449	Msa0251530:CDS
AAGACACTTGTTTATTACAA+AGG	0.562015	2_2:-41260973	Msa0251530:CDS
AAGACACTTGTTTATTACAA+AGG	0.562015	2_2:-41268419	Msa0251530:CDS
AAGAGAAGTAATGCAAACTA+TGG	0.358125	2_2:-41259617	Msa0251530:CDS
AAGAGAAGTAATGCAAACTA+TGG	0.358125	2_2:-41267063	Msa0251530:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTTGGATACTTTCTATCTCT+TGG	+	chr2_2:41259732-41259751	None:intergenic	30.0%
ATATTCATGCATAACCCAAT+TGG	+	chr2_2:41259898-41259917	None:intergenic	30.0%
ATTTGTGGCATAATAAAGGA+GGG	-	chr2_2:41260215-41260234	Msa0251530:intron	30.0%
CATTAACTCCTAATTAGAAG+GGG	+	chr2_2:41260353-41260372	None:intergenic	30.0%
TTAAAGCAAGCACTAAACAA+CGG	+	chr2_2:41260565-41260584	None:intergenic	30.0%
ACAAGATTGGAAAAGGAAAA+GGG	+	chr2_2:41260867-41260886	None:intergenic	30.0%
AACAAGATTGGAAAAGGAAA+AGG	+	chr2_2:41260868-41260887	None:intergenic	30.0%
GAAAATTCGACACAATGAAT+AGG	+	chr2_2:41261060-41261079	None:intergenic	30.0%
TAATGAAGAGGAACAAGTTA+AGG	-	chr2_2:41261109-41261128	Msa0251530:CDS	30.0%
ACAAGTTAAGGATACAAAGA+AGG	-	chr2_2:41261121-41261140	Msa0251530:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_2	gene	41259411	41268879	41259411	ID=Msa0251530;Name=Msa0251530
chr2_2	mRNA	41259411	41268879	41259411	ID=Msa0251530-mRNA-1;Parent=Msa0251530;Name=Msa0251530-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0\|0.4\|0\|1\|0.8\|0.83\|6\|0\|548
chr2_2	exon	41268723	41268879	41268723	ID=Msa0251530-mRNA-1:exon:13005;Parent=Msa0251530-mRNA-1
chr2_2	exon	41268328	41268608	41268328	ID=Msa0251530-mRNA-1:exon:13004;Parent=Msa0251530-mRNA-1
chr2_2	exon	41266857	41267237	41266857	ID=Msa0251530-mRNA-1:exon:13003;Parent=Msa0251530-mRNA-1
chr2_2	exon	41261277	41261439	41261277	ID=Msa0251530-mRNA-1:exon:13002;Parent=Msa0251530-mRNA-1
chr2_2	exon	41260882	41261162	41260882	ID=Msa0251530-mRNA-1:exon:13001;Parent=Msa0251530-mRNA-1
chr2_2	exon	41259411	41259791	41259411	ID=Msa0251530-mRNA-1:exon:13000;Parent=Msa0251530-mRNA-1
chr2_2	CDS	41268723	41268879	41268723	ID=Msa0251530-mRNA-1:cds;Parent=Msa0251530-mRNA-1
chr2_2	CDS	41268328	41268608	41268328	ID=Msa0251530-mRNA-1:cds;Parent=Msa0251530-mRNA-1
chr2_2	CDS	41266857	41267237	41266857	ID=Msa0251530-mRNA-1:cds;Parent=Msa0251530-mRNA-1
chr2_2	CDS	41261277	41261439	41261277	ID=Msa0251530-mRNA-1:cds;Parent=Msa0251530-mRNA-1
chr2_2	CDS	41260882	41261162	41260882	ID=Msa0251530-mRNA-1:cds;Parent=Msa0251530-mRNA-1
chr2_2	CDS	41259411	41259791	41259411	ID=Msa0251530-mRNA-1:cds;Parent=Msa0251530-mRNA-1

Gene Sequence

>Msa0251530

ATGGCTCCAATGACTCTACCACCAGGATTCAGGTTCCACCCTACAGATGAAGAACTTGTTGCTTACTACTTAGAAAGAAAAATAACAGGAAGAACTATAGAGCTGGATATCATTGCAGAAGTTGATCTCTACAAATGTGAACCTTGGGATTTACCAGAAAGATCATTTCTTCCTAGCAAGGATATGGAGTGGTATTTTTATAGTCCAAGAGATAGAAAGTATCCAAATGGGTCAAGAACAAATAGAGCAACAAGAGGTGGTTATTGGAAAGCAACTGGAAAAGATAGAGCAGTGAACTCTCAAAAGAGACAAGTTGGAATGAAGAAGACACTTGTTTATTACAAAGGTAGAGCACCTCATGGGATTAGAACCAATTGGGTTATGCATGAATATCGTCTTATTGAATCACATCCTGGTGCTTCTCTCTCTTCTTTGAAGGATTCCTATTCATTGTGTCGAATTTTCAAGAAGACAATTCAAATTCCAACAAAAAATAATGAAGAGGAACAAGTTAAGGATACAAAGAAGGAAATGATGGTCTTAGAAGAACAAGTGCAAGGGGAAGACTCAAGTGGTGGCACAGAGATTTCAAGAGAAGTAATGCAAACTATGGATGAAAAAATTCTTAATCAAGATAAACATCCAAAATTTCCATGTGATGCTTCTTCATCTGAGGTCACTCAAGGAACATGTACACCAACAGAGACATGTAATAATATACCCACAGAGGATTTTCATGCACAATTTGCCTATGAAGAAGAAAATAGTTCAGCTATTTCTTATCCAATGGGAATAAGCTACCCCTCAAATTTATTTCAGCTTGCAATGGCTCCAATGACTCTACCACCAGGATTCAGGTTCCACCCTACAGATGAAGAACTTGTTGCTTACTACTTAGAAAGAAAAATAACAGGAAGAACTATAGAGCTGGATATCATTGCAGAAGTTGATCTCTACAAATGTGAACCTTGGGATTTACCAGAAAGATCATTTCTTCCTAGCAAGGATATGGAGTGGTATTTTTATAGTCCAAGAGATAGAAAGTATCCAAATGGGTCAAGAACAAATAGAGCAACAAGAGGTGGTTATTGGAAAGCAACTGGAAAAGATAGAGCAGTGAACTCTCAAAAGAAACAAGTTGGAATGAAGAAGACACTTGTTTATTACAAAGGTAGAGCACCTCATGGGATTAGAACCAATTGGGTTATGCATGAATATCGTCTTATTGAATCACATCCTGGTGCTTCTCTCTCTTCTTTGAAGGATTCCTATTCATTGTGTCGAATTTTCAAGAAGACAATTCAAATTCCAACAAAAAATAATGAAGAGGAACAAGTTAAGGATACAAAGAAGGAAATGATGGTCTTAGAAGAACAAGTGCAAGGGGAAGACTCAAGTGGTGGCACAGAGATTTCAAGAGAAGTAATGCAAACTATGGATGAAAAAATTCTTAATCAAGATAAACATCCAAAATTTCCATGTGATGCTTCTTCATCTGAGGTCACTCAAGGAACATGTACACCAACAGAGACATGTAATAATATACCCACAGAGGATTTTCATGCACAATTTGCCTATGAAGAAGAAAATAGTTCAGCTATTTCTTATCCAATGGGAATAAGCTACCCTTCAAATTTATTTCAG

Protein sequence

>Msa0251530

MAPMTLPPGFRFHPTDEELVAYYLERKITGRTIELDIIAEVDLYKCEPWDLPERSFLPSKDMEWYFYSPRDRKYPNGSRTNRATRGGYWKATGKDRAVNSQKRQVGMKKTLVYYKGRAPHGIRTNWVMHEYRLIESHPGASLSSLKDSYSLCRIFKKTIQIPTKNNEEEQVKDTKKEMMVLEEQVQGEDSSGGTEISREVMQTMDEKILNQDKHPKFPCDASSSEVTQGTCTPTETCNNIPTEDFHAQFAYEEENSSAISYPMGISYPSNLFQLAMAPMTLPPGFRFHPTDEELVAYYLERKITGRTIELDIIAEVDLYKCEPWDLPERSFLPSKDMEWYFYSPRDRKYPNGSRTNRATRGGYWKATGKDRAVNSQKKQVGMKKTLVYYKGRAPHGIRTNWVMHEYRLIESHPGASLSSLKDSYSLCRIFKKTIQIPTKNNEEEQVKDTKKEMMVLEEQVQGEDSSGGTEISREVMQTMDEKILNQDKHPKFPCDASSSEVTQGTCTPTETCNNIPTEDFHAQFAYEEENSSAISYPMGISYPSNLFQ