AlfalfaGEDB | Msa0254450

M. sativa cultivar zhongmu-4 / Msa0254450

KEGG: K00924|K04733
GO: GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0031347 GO:0031349 GO:0031974 GO:0031981 GO:0035821 GO:0036211 GO:0038023 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045088 GO:0045089 GO:0046777 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052031 GO:0052033 GO:0052166 GO:0052167 GO:0052169 GO:0052173 GO:0052200 GO:0052255 GO:0052257 GO:0052305 GO:0052306 GO:0052308 GO:0052509 GO:0052510 GO:0052552 GO:0052553 GO:0052555 GO:0052556 GO:0052564 GO:0052572 GO:0060089 GO:0065007 GO:0070013 GO:0071704 GO:0071944 GO:0075136 GO:0080134 GO:0098542 GO:0140096 GO:1901564
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0254450	AES66511.2	78.451	297	26	3	1	271	1	285	2.64e-157	453

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0254450	sp\|Q65XV8\|RK176_ORYSJ	49.813	267	102	6	32	269	44	307	1.67e-75	238

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0254450	G7IQZ0	78.451	297	26	3	1	271	1	285	1.26e-157	453

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0254450	MtrunA17_Chr2g0315141	67.266	278	19	3	20	271	31	262	1.20e-117	341
Msa0254450	MtrunA17_Chr3g0111631	55.346	318	90	8	1	271	3	315	1.84e-93	281
Msa0254450	MtrunA17_Chr3g0111581	54.639	291	98	8	10	269	18	305	7.48e-91	273
Msa0254450	MtrunA17_Chr5g0435211	55.556	261	84	6	32	263	49	306	4.36e-83	255
Msa0254450	MtrunA17_Chr3g0111551	49.329	298	115	8	1	269	1	291	5.77e-82	250
Msa0254450	MtrunA17_Chr2g0328171	50.388	258	99	5	40	271	59	313	2.71e-74	233
Msa0254450	MtrunA17_Chr7g0238671	47.887	284	112	7	27	281	42	318	1.24e-73	231
Msa0254450	MtrunA17_Chr3g0111591	63.587	184	58	4	23	200	18	198	3.09e-69	213
Msa0254450	MtrunA17_Chr5g0416311	46.512	258	103	6	40	269	61	311	6.41e-68	216
Msa0254450	MtrunA17_Chr2g0328211	42.763	304	134	8	1	269	1	299	1.48e-66	211

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0254450	AT2G39660.1	50.000	256	99	5	40	269	52	304	1.24e-74	233
Msa0254450	AT2G28930.2	47.119	295	124	6	4	269	15	306	5.93e-74	232
Msa0254450	AT2G28930.3	47.119	295	124	6	4	269	18	309	6.94e-74	232
Msa0254450	AT1G07570.1	49.638	276	107	6	23	269	33	305	8.01e-74	232
Msa0254450	AT1G07570.5	49.638	276	107	6	23	269	33	305	8.01e-74	232
Msa0254450	AT1G07570.2	49.638	276	107	6	23	269	33	305	8.01e-74	232
Msa0254450	AT1G07570.3	49.638	276	107	6	23	269	44	316	1.40e-73	231
Msa0254450	AT2G28930.1	47.119	295	124	6	4	269	26	317	1.75e-73	231
Msa0254450	AT5G02290.2	46.479	284	119	7	15	269	26	305	2.06e-73	230
Msa0254450	AT5G02290.1	46.479	284	119	7	15	269	26	305	2.06e-73	230

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Find 62 sgRNAs with CRISPR-Local

Find 276 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCAGTACCTGGTTTAG+TGG	0.481520	2_2:-47791805	None:intergenic
AAACCAGTACCTGGTTTAGT+GGG	0.527060	2_2:-47791804	None:intergenic
AACCAGTACCTGGTTTAGTG+GGG	0.609098	2_2:-47791803	None:intergenic
AAGGTTGCGTAATTAAGGGC+TGG	0.551495	2_2:+47791760	Msa0254450:CDS
AAGTGTGATGTGTATAGTTT+TGG	0.337214	2_2:+47793217	Msa0254450:CDS
ACAAATTCATATACCAAAAG+TGG	0.524959	2_2:-47792100	None:intergenic
ACCAGTACCTGGTTTAGTGG+GGG	0.665304	2_2:-47791802	None:intergenic
ACCTGCACATTATGTAAATA+AGG	0.341190	2_2:+47793270	Msa0254450:CDS
ACTGCTTAGAGGATGTCGAC+CGG	0.645200	2_2:+47792077	Msa0254450:CDS
ACTGGTTGGTTACTGCTTAG+AGG	0.382051	2_2:+47792066	Msa0254450:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATAGACAAAAATCAATGACC+TGG	+	chr2_2:47792015-47792034	Msa0254450:intron	30.0%
GTATATGAATTTGTGACCAT+GGG	+	chr2_2:47792109-47792128	Msa0254450:CDS	30.0%
TATTTCTTCCATGAACATAG+GGG	+	chr2_2:47792398-47792417	Msa0254450:intron	30.0%
GATGAAGTGAATATGATACA+CGG	+	chr2_2:47792694-47792713	Msa0254450:CDS	30.0%
TTTGATTACAACTATGTGCA+AGG	-	chr2_2:47793041-47793060	None:intergenic	30.0%
ACCTGCACATTATGTAAATA+AGG	+	chr2_2:47793270-47793289	Msa0254450:CDS	30.0%
ATAAACAAGATCTCAAGTCA+TGG	+	chr2_2:47793340-47793359	Msa0254450:CDS	30.0%
ATAGACAAAAATCAATGACC+TGG	+	chr2_2:47792015-47792034	Msa0254450:intron	30.0%
GTATATGAATTTGTGACCAT+GGG	+	chr2_2:47792109-47792128	Msa0254450:CDS	30.0%
TATTTCTTCCATGAACATAG+GGG	+	chr2_2:47792398-47792417	Msa0254450:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_2	gene	47791234	47793396	47791234	ID=Msa0254450;Name=Msa0254450
chr2_2	mRNA	47791234	47793396	47791234	ID=Msa0254450-mRNA-1;Parent=Msa0254450;Name=Msa0254450-mRNA-1;_AED=0.23;_eAED=0.23;_QI=0\|0\|0\|1\|1\|1\|6\|0\|283
chr2_2	exon	47791234	47791291	47791234	ID=Msa0254450-mRNA-1:exon:14495;Parent=Msa0254450-mRNA-1
chr2_2	exon	47791631	47791884	47791631	ID=Msa0254450-mRNA-1:exon:14496;Parent=Msa0254450-mRNA-1
chr2_2	exon	47792019	47792157	47792019	ID=Msa0254450-mRNA-1:exon:14497;Parent=Msa0254450-mRNA-1
chr2_2	exon	47792602	47792750	47792602	ID=Msa0254450-mRNA-1:exon:14498;Parent=Msa0254450-mRNA-1
chr2_2	exon	47792843	47792900	47792843	ID=Msa0254450-mRNA-1:exon:14499;Parent=Msa0254450-mRNA-1
chr2_2	exon	47793203	47793396	47793203	ID=Msa0254450-mRNA-1:exon:14500;Parent=Msa0254450-mRNA-1
chr2_2	CDS	47791234	47791291	47791234	ID=Msa0254450-mRNA-1:cds;Parent=Msa0254450-mRNA-1
chr2_2	CDS	47791631	47791884	47791631	ID=Msa0254450-mRNA-1:cds;Parent=Msa0254450-mRNA-1
chr2_2	CDS	47792019	47792157	47792019	ID=Msa0254450-mRNA-1:cds;Parent=Msa0254450-mRNA-1
chr2_2	CDS	47792602	47792750	47792602	ID=Msa0254450-mRNA-1:cds;Parent=Msa0254450-mRNA-1
chr2_2	CDS	47792843	47792900	47792843	ID=Msa0254450-mRNA-1:cds;Parent=Msa0254450-mRNA-1
chr2_2	CDS	47793203	47793396	47793203	ID=Msa0254450-mRNA-1:cds;Parent=Msa0254450-mRNA-1

Gene Sequence

>Msa0254450

ATGGGTGGCGGCTTTAGTGCAGGGTACATAACTGAGAGGCAAAGGCCTTCACATCAAGGTTTGAATTCCTCTTCATATGTGCTTCTCAATTCTAGGACCGATGATGAGTTCAACTGTTTGAAGTGTTTCACTTTGAAAGAAATAAAAAAGGCGACAAAGAACTTTGGTCGAGATAGTAAGGTGAGTGAAGGTTGCGTAATTAAGGGCTGGATCGACGAGCACACATTAGCCCCCACTAAACCAGGTACTGGTTTTGCAATTTCTGTGAAGTGGAGTGACAAACTGGGCTACCTTGGAAATAGTGACTGGCCGACAAAAATCAATGACCTGGACCAGCTGCGTCATCCTAATCTTGTGAAACTGGTTGGTTACTGCTTAGAGGATGTCGACCGGCCACTTTTGGTATATGAATTTGTGACCATGGGAAGTTTGGATAACCATTTGTTTAGAAGCGAAGGGGCTTCTAACTTTGAGTCACTCTCTTGGAAAATCCGAATGAAGATAGCTCTTGATGCTGCTAAGGGTCTTGCATTTCTTCACAGCGATGAAGTGAATATGATACACGGAGAATTAAAAACTTCTAAGATCTTGATTGATTCAAACTACAATGCAAAACTATCTGTTTTCGGGGTGACAAAAGCCGGATTAAAACCAAATAGTTCTTTAACCAAAAAGTGTGATGTGTATAGTTTTGGGGTTGTTCTCCTAGAGATTATGTCCGGCAGACCTGCACATTATGTAAATAAGGCATATAAGGAGCGTTTATCTGAATGGGCCAAGCCATTCCTCGTCGACATAAACAAGATCTCAAGTCATGGATTCAGATATAGAAGGCCAATACTCATTGCTTGA

Protein sequence

>Msa0254450

MGGGFSAGYITERQRPSHQGLNSSSYVLLNSRTDDEFNCLKCFTLKEIKKATKNFGRDSKVSEGCVIKGWIDEHTLAPTKPGTGFAISVKWSDKLGYLGNSDWPTKINDLDQLRHPNLVKLVGYCLEDVDRPLLVYEFVTMGSLDNHLFRSEGASNFESLSWKIRMKIALDAAKGLAFLHSDEVNMIHGELKTSKILIDSNYNAKLSVFGVTKAGLKPNSSLTKKCDVYSFGVVLLEIMSGRPAHYVNKAYKERLSEWAKPFLVDINKISSHGFRYRRPILIA*