AlfalfaGEDB | Msa0254740

M. sativa cultivar zhongmu-4 / Msa0254740

KEGG: -
GO: GO:0001101 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003700 GO:0006355 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009627 GO:0009682 GO:0009719 GO:0009725 GO:0009751 GO:0009753 GO:0009755 GO:0009814 GO:0009862 GO:0009863 GO:0009864 GO:0009867 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0031349 GO:0032870 GO:0035690 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0045087 GO:0045088 GO:0045089 GO:0045892 GO:0045934 GO:0046677 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048831 GO:0050776 GO:0050778 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0080090 GO:0080134 GO:0098542 GO:0140110 GO:1900055 GO:1900056 GO:1901698 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:1905622 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2001141
PFAM: PF03106.18

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0254740	XP_003596299.1	76.119	134	27	3	1	132	160	290	2.23e-61	200

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0254740	sp\|Q8GWF1\|WRK38_ARATH	49.180	61	27	1	2	62	123	179	2.74e-14	70.5

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0254740	G7IR29	76.119	134	27	3	1	132	160	290	1.07e-61	200

TFs/TRs:

Gene ID	Type	Classification
Msa0254740	TF	WRKY

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0254740	MtrunA17_Chr2g0315361	76.119	134	27	3	1	132	160	290	2.05e-65	200
Msa0254740	MtrunA17_Chr2g0315371	79.545	132	27	0	1	132	153	284	7.70e-63	194
Msa0254740	MtrunA17_Chr2g0315381	65.649	131	41	2	2	132	157	283	6.42e-53	169
Msa0254740	MtrunA17_Chr8g0350621	45.536	112	42	4	2	108	124	221	2.82e-21	85.9
Msa0254740	MtrunA17_Chr7g0244981	40.833	120	54	3	2	115	135	243	1.26e-20	84.7
Msa0254740	MtrunA17_Chr7g0244971	46.512	86	42	2	2	85	136	219	2.72e-19	81.3
Msa0254740	MtrunA17_Chr8g0350631	50.000	78	34	2	10	85	5	79	6.39e-19	77.4
Msa0254740	MtrunA17_Chr8g0334541	47.778	90	33	3	2	85	116	197	2.13e-18	78.6
Msa0254740	MtrunA17_Chr2g0315401	40.708	113	57	3	19	131	21	123	9.82e-17	71.6
Msa0254740	MtrunA17_Chr3g0128671	37.838	111	49	4	2	92	140	250	1.02e-14	68.9
Msa0254740	MtrunA17_Chr2g0315341	39.640	111	54	4	25	131	1	102	3.74e-14	64.3
Msa0254740	MtrunA17_Chr5g0428351	38.235	68	42	0	2	69	196	263	6.30e-12	61.2
Msa0254740	MtrunA17_Chr7g0275531	46.552	58	31	0	2	59	131	188	3.25e-11	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0254740	AT5G22570.1	49.180	61	27	1	2	62	123	179	2.79e-15	70.5
Msa0254740	AT5G01900.1	32.090	134	64	3	2	122	123	242	2.68e-14	67.8
Msa0254740	AT1G66560.1	54.545	55	24	1	2	55	116	170	5.32e-14	66.6
Msa0254740	AT1G66550.2	50.909	55	26	1	2	55	116	170	2.58e-13	64.7
Msa0254740	AT1G66550.1	50.909	55	26	1	2	55	121	175	3.09e-13	64.7
Msa0254740	AT1G80590.1	48.980	49	25	0	2	50	98	146	5.82e-12	60.8
Msa0254740	AT1G66600.1	53.061	49	23	0	2	50	116	164	6.05e-12	60.8
Msa0254740	AT3G56400.1	48.936	47	24	0	2	48	133	179	5.32e-11	58.9

Find 25 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTACCATTCAGACTTAAATT+TGG	0.184409	2_2:+48326632	Msa0254740:CDS
AATTAAGTACCTGAAATATT+TGG	0.227860	2_2:-48326210	None:intergenic
TAACAAATGCCAAATATTTC+AGG	0.288144	2_2:+48326201	Msa0254740:CDS
TTGTACAGGACTACATATTA+TGG	0.292618	2_2:+48326588	Msa0254740:CDS
AGTTCTTTGAACATGTTTGA+TGG	0.334201	2_2:-48326550	None:intergenic
ATTGTTATCAGAGGAGAAAA+TGG	0.344422	2_2:-48326658	None:intergenic
GATTCTCCGATGCGAACAAT+TGG	0.447503	2_2:-48326827	None:intergenic
ATTCCAAATTTAAGTCTGAA+TGG	0.467467	2_2:-48326635	None:intergenic
ATGTATGCAGGAATTACTAT+AGG	0.486763	2_2:+48326496	Msa0254740:intron
GATGGTGGTCGCGGTCGAAA+TGG	0.511390	2_2:-48326729	None:intergenic
CTTGAAATGTTGTTATCGAA+AGG	0.514935	2_2:-48326693	None:intergenic
GCGACCACCATCGCCACCAC+TGG	0.527513	2_2:+48326740	Msa0254740:CDS
ATCTACAAAAGAGGAATACA+AGG	0.555372	2_2:+48326773	Msa0254740:CDS
AAGAGAACCCTCCTTTGTAC+AGG	0.557218	2_2:+48326574	Msa0254740:CDS
TGGAGACCAATTGTTCGCAT+CGG	0.562235	2_2:+48326821	Msa0254740:CDS
AGTGCCAGTGGTGGCGATGG+TGG	0.571240	2_2:-48326744	None:intergenic
ACTTCTTGCATCTACAAAAG+AGG	0.575411	2_2:+48326764	Msa0254740:CDS
ATGTAGTCCTGTACAAAGGA+GGG	0.583465	2_2:-48326581	None:intergenic
AGAAGTGCCAGTGGTGGCGA+TGG	0.586410	2_2:-48326747	None:intergenic
AGTGGTGGCGATGGTGGTCG+CGG	0.597527	2_2:-48326738	None:intergenic
TATGTAGTCCTGTACAAAGG+AGG	0.603277	2_2:-48326582	None:intergenic
TAATATAGAATTGTTATCAG+AGG	0.605864	2_2:-48326667	None:intergenic
TAATATGTAGTCCTGTACAA+AGG	0.665931	2_2:-48326585	None:intergenic
GTAGATGCAAGAAGTGCCAG+TGG	0.719591	2_2:-48326756	None:intergenic
GATGCAAGAAGTGCCAGTGG+TGG	0.724195	2_2:-48326753	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTTATAATTCAAAACTTAAT+TGG	+	chr2_2:48326469-48326488	Msa0254740:intron	10.0%
!!	AATTAAGTACCTGAAATATT+TGG	-	chr2_2:48326213-48326232	None:intergenic	20.0%
!!	ATTACAATTATATCTAAGAG+TGG	-	chr2_2:48326391-48326410	None:intergenic	20.0%
!!	TAATATAGAATTGTTATCAG+AGG	-	chr2_2:48326670-48326689	None:intergenic	20.0%
!	TAACAAATGCCAAATATTTC+AGG	+	chr2_2:48326201-48326220	Msa0254740:CDS	25.0%
!	TTGGAACAATAGTCAAAATT+AGG	+	chr2_2:48326290-48326309	Msa0254740:intron	25.0%
!!	TTAATTGGTAATATGTATGC+AGG	+	chr2_2:48326484-48326503	Msa0254740:intron	25.0%
!	TTACCATTCAGACTTAAATT+TGG	+	chr2_2:48326632-48326651	Msa0254740:CDS	25.0%
!	ATTCCAAATTTAAGTCTGAA+TGG	-	chr2_2:48326638-48326657	None:intergenic	25.0%
!!	ATCCAGAATGATAATTTTTC+TGG	+	chr2_2:48326801-48326820	Msa0254740:CDS	25.0%
!	ATAAACAAAAATCGCATAAC+TGG	-	chr2_2:48326854-48326873	None:intergenic	25.0%
	ATGTATGCAGGAATTACTAT+AGG	+	chr2_2:48326496-48326515	Msa0254740:intron	30.0%
	AGTTCTTTGAACATGTTTGA+TGG	-	chr2_2:48326553-48326572	None:intergenic	30.0%
	TAATATGTAGTCCTGTACAA+AGG	-	chr2_2:48326588-48326607	None:intergenic	30.0%
	TTGTACAGGACTACATATTA+TGG	+	chr2_2:48326588-48326607	Msa0254740:CDS	30.0%
	ATTGTTATCAGAGGAGAAAA+TGG	-	chr2_2:48326661-48326680	None:intergenic	30.0%
	CTTGAAATGTTGTTATCGAA+AGG	-	chr2_2:48326696-48326715	None:intergenic	30.0%
	ATCTACAAAAGAGGAATACA+AGG	+	chr2_2:48326773-48326792	Msa0254740:CDS	30.0%
	CTCCAGAAAAATTATCATTC+TGG	-	chr2_2:48326806-48326825	None:intergenic	30.0%
	GACTAATCTTGATTCTCAAG+TGG	-	chr2_2:48326255-48326274	None:intergenic	35.0%
	AGTCAACATCATGTACTTGT+TGG	+	chr2_2:48326271-48326290	Msa0254740:intron	35.0%
!	AAGAGCTTTTGCAAATGTGA+TGG	-	chr2_2:48326614-48326633	None:intergenic	35.0%
	ACTTCTTGCATCTACAAAAG+AGG	+	chr2_2:48326764-48326783	Msa0254740:CDS	35.0%
	GATTCTCAAGTGGATGTAGA+TGG	-	chr2_2:48326245-48326264	None:intergenic	40.0%
	TCATCGAAGAGTCGTAGATT+AGG	+	chr2_2:48326329-48326348	Msa0254740:intron	40.0%
	ATGTAGTCCTGTACAAAGGA+GGG	-	chr2_2:48326584-48326603	None:intergenic	40.0%
	TATGTAGTCCTGTACAAAGG+AGG	-	chr2_2:48326585-48326604	None:intergenic	40.0%
	AAGAGAACCCTCCTTTGTAC+AGG	+	chr2_2:48326574-48326593	Msa0254740:CDS	45.0%
	TGGAGACCAATTGTTCGCAT+CGG	+	chr2_2:48326821-48326840	Msa0254740:CDS	45.0%
	GATTCTCCGATGCGAACAAT+TGG	-	chr2_2:48326830-48326849	None:intergenic	45.0%
!!	GTAGATGCAAGAAGTGCCAG+TGG	-	chr2_2:48326759-48326778	None:intergenic	50.0%
!!	GATGCAAGAAGTGCCAGTGG+TGG	-	chr2_2:48326756-48326775	None:intergenic	55.0%
!	GATGGTGGTCGCGGTCGAAA+TGG	-	chr2_2:48326732-48326751	None:intergenic	60.0%
!!	AGAAGTGCCAGTGGTGGCGA+TGG	-	chr2_2:48326750-48326769	None:intergenic	60.0%
!	AGTGGTGGCGATGGTGGTCG+CGG	-	chr2_2:48326741-48326760	None:intergenic	65.0%
!	AGTGCCAGTGGTGGCGATGG+TGG	-	chr2_2:48326747-48326766	None:intergenic	65.0%
	GCGACCACCATCGCCACCAC+TGG	+	chr2_2:48326740-48326759	Msa0254740:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_2	gene	48326197	48326878	48326197	ID=Msa0254740;Name=Msa0254740
chr2_2	mRNA	48326197	48326878	48326197	ID=Msa0254740-mRNA-1;Parent=Msa0254740;Name=Msa0254740-mRNA-1;_AED=0.15;_eAED=0.16;_QI=0\|0\|0\|1\|1\|1\|2\|0\|132
chr2_2	exon	48326197	48326222	48326197	ID=Msa0254740-mRNA-1:exon:14574;Parent=Msa0254740-mRNA-1
chr2_2	exon	48326506	48326878	48326506	ID=Msa0254740-mRNA-1:exon:14575;Parent=Msa0254740-mRNA-1
chr2_2	CDS	48326197	48326222	48326197	ID=Msa0254740-mRNA-1:cds;Parent=Msa0254740-mRNA-1
chr2_2	CDS	48326506	48326878	48326506	ID=Msa0254740-mRNA-1:cds;Parent=Msa0254740-mRNA-1

Gene Sequence

>Msa0254740

ATGATAACAAATGCCAAATATTTCAGGAATTACTATAGGTGCACTCACATGTACGATCAACATTGTGAAGCCATCAAACATGTTCAAAGAACTCAAGAGAACCCTCCTTTGTACAGGACTACATATTATGGCCATCACATTTGCAAAAGCTCTTACCATTCAGACTTAAATTTGGAATCCATTTTCTCCTCTGATAACAATTCTATATTACTTCCTTTCGATAACAACATTTCAAGTGAACAAGAATATCCATTTCGACCGCGACCACCATCGCCACCACTGGCACTTCTTGCATCTACAAAAGAGGAATACAAGGAAGTGATCCAGAATGATAATTTTTCTGGAGACCAATTGTTCGCATCGGAGAATCTCCAGTTATGCGATTTTTGTTTATTTTGA

Protein sequence

>Msa0254740

MITNAKYFRNYYRCTHMYDQHCEAIKHVQRTQENPPLYRTTYYGHHICKSSYHSDLNLESIFSSDNNSILLPFDNNISSEQEYPFRPRPPSPPLALLASTKEEYKEVIQNDNFSGDQLFASENLQLCDFCLF*