AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0255040

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0255040 MtrunA17_Chr2g0315651 93.460 367 24 0 1 367 1 367 0.0 724
Msa0255040 MtrunA17_Chr2g0315641 73.925 372 88 5 1 367 4 371 0.0 551
Msa0255040 MtrunA17_Chr2g0286441 46.011 376 163 9 21 367 29 393 7.05e-104 311
Msa0255040 MtrunA17_Chr5g0404321 45.789 380 165 10 25 367 41 416 1.01e-102 309
Msa0255040 MtrunA17_Chr8g0373001 46.011 376 166 7 25 367 41 412 1.13e-100 303
Msa0255040 MtrunA17_Chr1g0201891 44.588 388 153 11 35 367 51 431 9.34e-98 297
Msa0255040 MtrunA17_Chr1g0196071 44.828 377 169 10 21 367 35 402 2.41e-95 290
Msa0255040 MtrunA17_Chr2g0278511 43.272 379 162 10 37 367 54 427 6.35e-95 289
Msa0255040 MtrunA17_Chr1g0155301 44.944 356 168 8 37 367 70 422 6.94e-92 281
Msa0255040 MtrunA17_Chr8g0373361 37.027 370 189 9 25 360 46 405 2.59e-64 210
Msa0255040 MtrunA17_Chr8g0373331 37.640 356 155 11 25 360 40 348 9.70e-53 178
Msa0255040 MtrunA17_Chr8g0373341 35.583 326 156 8 34 357 47 320 1.40e-38 140
Msa0255040 MtrunA17_Chr1g0156151 46.043 139 71 1 52 186 66 204 6.20e-33 122
Msa0255040 MtrunA17_Chr8g0373351 33.033 333 138 12 44 359 22 286 2.03e-27 109
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0255040 AT1G53320.1 65.994 347 107 4 29 367 36 379 4.66e-157 446
Msa0255040 AT2G18280.2 45.730 363 174 6 25 367 34 393 9.03e-101 303
Msa0255040 AT2G18280.1 45.730 363 174 6 25 367 34 393 9.03e-101 303
Msa0255040 AT2G47900.2 43.889 360 168 7 37 367 52 406 9.22e-100 301
Msa0255040 AT2G47900.1 43.889 360 168 7 37 367 52 406 9.22e-100 301
Msa0255040 AT2G47900.3 43.767 361 168 7 37 367 52 407 1.78e-99 301
Msa0255040 AT1G76900.2 42.015 407 158 9 34 367 54 455 7.92e-98 298
Msa0255040 AT1G76900.1 42.015 407 158 9 34 367 54 455 7.92e-98 298
Msa0255040 AT1G76900.3 42.015 407 158 9 34 367 54 455 7.92e-98 298
Msa0255040 AT1G25280.1 41.278 407 175 10 21 367 43 445 4.74e-95 291
Msa0255040 AT3G06380.1 44.722 360 166 9 33 367 29 380 3.67e-94 286
Msa0255040 AT1G47270.3 43.978 357 170 9 33 367 40 388 2.16e-89 274
Msa0255040 AT1G47270.2 43.978 357 170 9 33 367 64 412 4.32e-89 274
Msa0255040 AT1G47270.1 43.978 357 170 9 33 367 65 413 4.54e-89 274
Msa0255040 AT1G43640.1 41.818 385 164 11 35 367 53 429 2.97e-87 270
Msa0255040 AT5G18680.1 43.478 368 177 11 25 367 28 389 4.49e-87 268
Msa0255040 AT1G43640.2 41.779 371 156 11 49 367 1 363 1.72e-83 258
Msa0255040 AT3G06380.2 43.151 292 134 8 100 367 5 288 9.09e-68 215
Msa0255040 AT5G18680.2 43.197 294 138 9 98 367 9 297 9.03e-65 208
Msa0255040 AT1G25280.3 39.483 271 101 9 156 367 1 267 1.09e-50 171
Msa0255040 AT1G25280.2 39.483 271 101 9 156 367 1 267 1.09e-50 171
Msa0255040 AT1G61940.2 48.276 87 45 0 40 126 1 87 1.44e-22 96.3
Msa0255040 AT1G61940.1 49.180 61 31 0 40 100 1 61 1.12e-14 73.6

Find 90 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCACCACTGTGTTGTAAAT+TGG 0.174041 2_2:+48654928 Msa0255040:CDS
CGGCTTGTTCTGTCATTTAA+TGG 0.192126 2_2:-48655553 None:intergenic
GCTTGTCAGTAAGATCATTT+TGG 0.266008 2_2:+48656072 Msa0255040:exon
ACCATTCGCCTTGGACGTTT+TGG 0.266261 2_2:-48655667 None:intergenic
CTTTCCCTCCTGGTTGGCTT+TGG 0.277699 2_2:-48655921 None:intergenic
TCGCCAGCATTAACAGATAA+AGG 0.294918 2_2:+48655148 Msa0255040:intron
ATAATATCCGCAAGAAGTTC+TGG 0.306087 2_2:-48653920 None:intergenic
TGTTCTGTCATTTAATGGTT+TGG 0.319298 2_2:-48655548 None:intergenic
GAACGCATGGAAGGCGGATA+AGG 0.334268 2_2:-48656016 None:intergenic
TCTTCATCTCCTTTCCCTCC+TGG 0.342544 2_2:-48655931 None:intergenic
TTTCCCTTCTTGTCTTAAAC+AGG 0.347974 2_2:+48654075 Msa0255040:CDS
GATATCTTCTAGCTGCTAAT+AGG 0.356446 2_2:-48655177 None:intergenic
AACTAGCTTCGGCACTAAAC+TGG 0.357473 2_2:+48656050 Msa0255040:CDS
GCCATTTGCTTAACTAGCTT+CGG 0.361202 2_2:+48656039 Msa0255040:CDS
ATAAGCCCTCAAGTTCCTTC+TGG 0.387424 2_2:+48655595 Msa0255040:CDS
TATTAGCAGCTAGAAGATAT+CGG 0.406159 2_2:+48655179 Msa0255040:CDS
TGAGGAAGGTCGCGTGGCCC+TGG 0.406999 2_2:-48654909 None:intergenic
CGCCAGCATTAACAGATAAA+GGG 0.417908 2_2:+48655149 Msa0255040:intron
GTACTTACTTACATGGAGTA+AGG 0.424832 2_2:-48654986 None:intergenic
ATGTTACTATCAATTATTTG+TGG 0.443910 2_2:+48656254 Msa0255040:three_prime_UTR
CATCTCCTTTCCCTCCTGGT+TGG 0.445642 2_2:-48655927 None:intergenic
ATAATTTATGATGCCTCGGC+TGG 0.449497 2_2:+48655523 Msa0255040:CDS
AACGCATGGAAGGCGGATAA+GGG 0.450456 2_2:-48656015 None:intergenic
CTGTCATATCCTTTCTCTCA+TGG 0.452722 2_2:-48655858 None:intergenic
GTAGGGTCACCTTCTCTTAA+CGG 0.460650 2_2:+48654043 Msa0255040:CDS
TGAGTTTATTATTTGATTGA+AGG 0.464459 2_2:+48656175 Msa0255040:three_prime_UTR
CTTGAACTTCCATGAGAGAA+AGG 0.465557 2_2:+48655849 Msa0255040:CDS
CAAGGAAGTAATGCTTATGT+TGG 0.475540 2_2:+48655253 Msa0255040:CDS
CAAAGCCAACCAGGAGGGAA+AGG 0.481638 2_2:+48655922 Msa0255040:CDS
CCTTCTGGTAACTTCGAGGC+AGG 0.487946 2_2:+48655610 Msa0255040:CDS
CTTGTGCCTGCGTTTGCAAA+CGG 0.489375 2_2:+48653996 Msa0255040:CDS
CTTTCGCATATTGATGAGGC+AGG 0.503626 2_2:-48655706 None:intergenic
ACGATGCGGCCATTGTTCTC+CGG 0.508756 2_2:-48653960 None:intergenic
CTTCATAATTTATGATGCCT+CGG 0.511164 2_2:+48655519 Msa0255040:CDS
GCCGAAGCTAGTTAAGCAAA+TGG 0.515952 2_2:-48656040 None:intergenic
TTTGTCCTCCAATGCACTAT+CGG 0.518155 2_2:-48653876 None:intergenic
AGAGAGCAAACCATTCGCCT+TGG 0.518911 2_2:-48655676 None:intergenic
CAGATACTTCAACAATGCTC+AGG 0.525873 2_2:+48653826 Msa0255040:CDS
GCTCAGGCTCTCAATGCAAC+CGG 0.530381 2_2:+48653842 Msa0255040:CDS
CACCACCGATAGTGCATTGG+AGG 0.531810 2_2:+48653871 Msa0255040:CDS
ATGCTTATGTTGGCAAGCTA+AGG 0.535171 2_2:+48655263 Msa0255040:CDS
CATTGGAGGACAAAACTGAT+TGG 0.540142 2_2:+48653885 Msa0255040:CDS
ATGGAGTAAGGGCAAGATAG+AGG 0.540432 2_2:-48654974 None:intergenic
AGGACCTGTTTAAGACAAGA+AGG 0.544045 2_2:-48654079 None:intergenic
CGCAAGAAGTTCTGGAAGCA+TGG 0.545732 2_2:-48653912 None:intergenic
ATATCACACGTGAGGTCGTA+GGG 0.551715 2_2:+48654026 Msa0255040:CDS
GGAACGAGCAATTAGAGTGC+TGG 0.557590 2_2:+48655824 Msa0255040:CDS
TTAAGCAAATGGCGAACGCA+TGG 0.557939 2_2:-48656029 None:intergenic
TAATGGTTTGGCACCAGCCG+AGG 0.560048 2_2:-48655536 None:intergenic
GGCAAGATAGAGGTAAAATG+TGG 0.562187 2_2:-48654964 None:intergenic
TCTAATTGCTCGTTCCACAT+TGG 0.562401 2_2:-48655817 None:intergenic
TTTGTTCTTCAAGATTGTGT+AGG 0.566095 2_2:-48655791 None:intergenic
AAGTTACCAGAAGGAACTTG+AGG 0.573898 2_2:-48655601 None:intergenic
AGTTCCTTCTGGTAACTTCG+AGG 0.573958 2_2:+48655606 Msa0255040:CDS
ATTAAACGAGTTGATGTCAC+CGG 0.574864 2_2:+48653941 Msa0255040:CDS
ACCAAAACGTCCAAGGCGAA+TGG 0.576531 2_2:+48655666 Msa0255040:CDS
ACTATCGGTGGTGTCATCGC+CGG 0.586160 2_2:-48653861 None:intergenic
TACTTACTTACATGGAGTAA+GGG 0.588591 2_2:-48654985 None:intergenic
AGTTACCAGAAGGAACTTGA+GGG 0.589028 2_2:-48655600 None:intergenic
TGACACCACCGATAGTGCAT+TGG 0.590958 2_2:+48653868 Msa0255040:CDS
ACTTCCTTGTGAAAAGTCAC+CGG 0.591079 2_2:-48655239 None:intergenic
GATATCACACGTGAGGTCGT+AGG 0.591293 2_2:+48654025 Msa0255040:CDS
GCAAATGGCGAACGCATGGA+AGG 0.591902 2_2:-48656025 None:intergenic
AATAAACTCAGCCAAGTGAA+AGG 0.592578 2_2:-48656162 None:intergenic
ACTGTGGACCAAAGCCAACC+AGG 0.595427 2_2:+48655913 Msa0255040:CDS
CTTCTGGTAACTTCGAGGCA+GGG 0.597523 2_2:+48655611 Msa0255040:CDS
TCTGTTTGCTTGCAAAACGC+CGG 0.603910 2_2:-48655573 None:intergenic
GTGCCTGCGTTTGCAAACGG+TGG 0.610120 2_2:+48653999 Msa0255040:CDS
GTCCTCCAATGCACTATCGG+TGG 0.615249 2_2:-48653873 None:intergenic
TAAGGGCTGTCTATAGTACA+TGG 0.615567 2_2:-48655998 None:intergenic
CAGTGGTGAGGAAGGTCGCG+TGG 0.622650 2_2:-48654915 None:intergenic
CATGCTTCCAGAACTTCTTG+CGG 0.624235 2_2:+48653913 Msa0255040:CDS
TGAACTTTCGCATATTGATG+AGG 0.626688 2_2:-48655710 None:intergenic
TTACAACACAGTGGTGAGGA+AGG 0.627102 2_2:-48654923 None:intergenic
TCTCCACCGTTTGCAAACGC+AGG 0.641600 2_2:-48654002 None:intergenic
GGACCTGTTTAAGACAAGAA+GGG 0.643301 2_2:-48654078 None:intergenic
AATGGCGAACGCATGGAAGG+CGG 0.645991 2_2:-48656022 None:intergenic
CAATTTACAACACAGTGGTG+AGG 0.647243 2_2:-48654927 None:intergenic
TGAAGAACAAAGCTCCAATG+TGG 0.651252 2_2:+48655803 Msa0255040:CDS
CCCCTACTTTACAAAACTGG+AGG 0.654395 2_2:-48655963 None:intergenic
TGGACCAAAGCCAACCAGGA+GGG 0.658819 2_2:+48655917 Msa0255040:CDS
AAGATAGAGGTAAAATGTGG+AGG 0.660216 2_2:-48654961 None:intergenic
TATGTTATATCACTTGATGC+CGG 0.661326 2_2:+48655220 Msa0255040:CDS
CATTAAATGACAGAACAAGC+CGG 0.669123 2_2:+48655554 Msa0255040:CDS
CCTGCCTCGAAGTTACCAGA+AGG 0.676338 2_2:-48655610 None:intergenic
GCGACGACATTTCGACGATG+CGG 0.691215 2_2:-48653974 None:intergenic
TTGATGTCACCGGAGAACAA+TGG 0.692340 2_2:+48653951 Msa0255040:CDS
TATTCCAATTTACAACACAG+TGG 0.695058 2_2:-48654932 None:intergenic
GTGGAGAGATATCACACGTG+AGG 0.702332 2_2:+48654018 Msa0255040:CDS
GTGGACCAAAGCCAACCAGG+AGG 0.734579 2_2:+48655916 Msa0255040:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATGATTAAAATTTCAAA+TGG + chr2_2:48655042-48655061 Msa0255040:intron 10.0%
!!! CAATATATGGTTTTTTTTAA+TGG - chr2_2:48655409-48655428 None:intergenic 15.0%
!!! TGTTAATGTTATATTGTTTT+TGG + chr2_2:48656212-48656231 Msa0255040:three_prime_UTR 15.0%
!! TTTCTCTCTTGAATAATTTA+TGG + chr2_2:48654126-48654145 Msa0255040:intron 20.0%
!! TTATGGATCATGAAAAAAAT+TGG + chr2_2:48654143-48654162 Msa0255040:intron 20.0%
!!! ATCTTGTTATGTTTTGATAA+AGG + chr2_2:48654462-48654481 Msa0255040:intron 20.0%
!! TCAAACTATTAAAGAAGAAA+AGG - chr2_2:48654699-48654718 None:intergenic 20.0%
!! TTAAAATTTCAAATGGAGAT+TGG + chr2_2:48655049-48655068 Msa0255040:intron 20.0%
!!! AACTCTTTTTCTACAATAAA+TGG - chr2_2:48655317-48655336 None:intergenic 20.0%
!! ATTTATTGTAGAAAAAGAGT+TGG + chr2_2:48655316-48655335 Msa0255040:intron 20.0%
!! TTGAGAACAAATTCAATATA+TGG - chr2_2:48655422-48655441 None:intergenic 20.0%
!!! ATTCACTTTTTAACATTGAA+AGG - chr2_2:48656121-48656140 None:intergenic 20.0%
!! TGAGTTTATTATTTGATTGA+AGG + chr2_2:48656175-48656194 Msa0255040:three_prime_UTR 20.0%
!! ATGTTACTATCAATTATTTG+TGG + chr2_2:48656254-48656273 Msa0255040:three_prime_UTR 20.0%
!! AAATGAATTCATTTTGTTGC+AGG - chr2_2:48654102-48654121 None:intergenic 25.0%
!! CTAACATTTTGTTCAATTAG+TGG + chr2_2:48654201-48654220 Msa0255040:intron 25.0%
! ATCAATTGAATTAAGAGAGT+TGG + chr2_2:48654430-48654449 Msa0255040:intron 25.0%
! TAAACTCCAATTAAGTTAAC+TGG + chr2_2:48654552-48654571 Msa0255040:intron 25.0%
!! CATTTTCCAGTTAACTTAAT+TGG - chr2_2:48654561-48654580 None:intergenic 25.0%
! TAAGAGTGAACAAATTCATT+AGG - chr2_2:48654608-48654627 None:intergenic 25.0%
!! TTTTGGCAAAAGGATTATTT+GGG + chr2_2:48655011-48655030 Msa0255040:intron 25.0%
! TTAATATTTACTCTTTCGTC+CGG - chr2_2:48655360-48655379 None:intergenic 25.0%
AAAATGAAGCCAAGTTTACT+CGG + chr2_2:48654575-48654594 Msa0255040:intron 30.0%
TATTCCAATTTACAACACAG+TGG - chr2_2:48654935-48654954 None:intergenic 30.0%
TACTTACTTACATGGAGTAA+GGG - chr2_2:48654988-48655007 None:intergenic 30.0%
CAAAACAGTACTTACTTACA+TGG - chr2_2:48654996-48655015 None:intergenic 30.0%
!! CATGTAAGTAAGTACTGTTT+TGG + chr2_2:48654994-48655013 Msa0255040:intron 30.0%
!! GTTTTGGCAAAAGGATTATT+TGG + chr2_2:48655010-48655029 Msa0255040:intron 30.0%
TATTAGCAGCTAGAAGATAT+CGG + chr2_2:48655179-48655198 Msa0255040:CDS 30.0%
TATGTTATATCACTTGATGC+CGG + chr2_2:48655220-48655239 Msa0255040:CDS 30.0%
TATTGTAGAAAAAGAGTTGG+AGG + chr2_2:48655319-48655338 Msa0255040:intron 30.0%
TAATATTTACTCTTTCGTCC+GGG - chr2_2:48655359-48655378 None:intergenic 30.0%
CTTCATAATTTATGATGCCT+CGG + chr2_2:48655519-48655538 Msa0255040:CDS 30.0%
! TGTTCTGTCATTTAATGGTT+TGG - chr2_2:48655551-48655570 None:intergenic 30.0%
!!! CAAAAGGCTGAATTTTTTGT+AGG - chr2_2:48655644-48655663 None:intergenic 30.0%
TTTGTTCTTCAAGATTGTGT+AGG - chr2_2:48655794-48655813 None:intergenic 30.0%
!!! GTTCTTTTTTTCCTTTCACT+TGG + chr2_2:48656151-48656170 Msa0255040:three_prime_UTR 30.0%
ATAATATCCGCAAGAAGTTC+TGG - chr2_2:48653923-48653942 None:intergenic 35.0%
!! ATTAAACGAGTTGATGTCAC+CGG + chr2_2:48653941-48653960 Msa0255040:CDS 35.0%
! GTGATTTTTCCGTTAAGAGA+AGG - chr2_2:48654055-48654074 None:intergenic 35.0%
TTTCCCTTCTTGTCTTAAAC+AGG + chr2_2:48654075-48654094 Msa0255040:CDS 35.0%
AAAATCACGAGTTAACTCGT+AGG + chr2_2:48654490-48654509 Msa0255040:intron 35.0%
!! ACTCTTGATTTTGACAACCT+TGG + chr2_2:48654870-48654889 Msa0255040:intron 35.0%
AAGATAGAGGTAAAATGTGG+AGG - chr2_2:48654964-48654983 None:intergenic 35.0%
GTACTTACTTACATGGAGTA+AGG - chr2_2:48654989-48655008 None:intergenic 35.0%
!!! GTAAGTACTGTTTTGGCAAA+AGG + chr2_2:48655001-48655020 Msa0255040:intron 35.0%
TTCCCTTTATCTGTTAATGC+TGG - chr2_2:48655154-48655173 None:intergenic 35.0%
! GATATCTTCTAGCTGCTAAT+AGG - chr2_2:48655180-48655199 None:intergenic 35.0%
CAAGGAAGTAATGCTTATGT+TGG + chr2_2:48655253-48655272 Msa0255040:CDS 35.0%
CATTAAATGACAGAACAAGC+CGG + chr2_2:48655554-48655573 Msa0255040:CDS 35.0%
!!! TTGGACGTTTTGGTTTCAAA+AGG - chr2_2:48655660-48655679 None:intergenic 35.0%
!!! TTTTGAAACCAAAACGTCCA+AGG + chr2_2:48655659-48655678 Msa0255040:CDS 35.0%
TGAACTTTCGCATATTGATG+AGG - chr2_2:48655713-48655732 None:intergenic 35.0%
! ATCAGTGAAGAATTTTCAGC+TGG + chr2_2:48655885-48655904 Msa0255040:CDS 35.0%
!! TCCTCCAGTTTTGTAAAGTA+GGG + chr2_2:48655962-48655981 Msa0255040:CDS 35.0%
! GCTTGTCAGTAAGATCATTT+TGG + chr2_2:48656072-48656091 Msa0255040:exon 35.0%
AATAAACTCAGCCAAGTGAA+AGG - chr2_2:48656165-48656184 None:intergenic 35.0%
CAGATACTTCAACAATGCTC+AGG + chr2_2:48653826-48653845 Msa0255040:CDS 40.0%
TTTGTCCTCCAATGCACTAT+CGG - chr2_2:48653879-48653898 None:intergenic 40.0%
CATTGGAGGACAAAACTGAT+TGG + chr2_2:48653885-48653904 Msa0255040:CDS 40.0%
! GGACCTGTTTAAGACAAGAA+GGG - chr2_2:48654081-48654100 None:intergenic 40.0%
! AGGACCTGTTTAAGACAAGA+AGG - chr2_2:48654082-48654101 None:intergenic 40.0%
TAGTGGCTATCTATGAAGCA+CGG + chr2_2:48654218-48654237 Msa0255040:intron 40.0%
CTATCTATGAAGCACGGATA+CGG + chr2_2:48654224-48654243 Msa0255040:intron 40.0%
TACAAGTGTCAGTGTCGTAT+CGG + chr2_2:48654314-48654333 Msa0255040:intron 40.0%
TAATAGCCACCTATGAAGCA+CGG - chr2_2:48654392-48654411 None:intergenic 40.0%
ACTCGTAGGATCGTATGTTT+AGG + chr2_2:48654504-48654523 Msa0255040:intron 40.0%
CAATTTACAACACAGTGGTG+AGG - chr2_2:48654930-48654949 None:intergenic 40.0%
CTCACCACTGTGTTGTAAAT+TGG + chr2_2:48654928-48654947 Msa0255040:CDS 40.0%
GGCAAGATAGAGGTAAAATG+TGG - chr2_2:48654967-48654986 None:intergenic 40.0%
TCGCCAGCATTAACAGATAA+AGG + chr2_2:48655148-48655167 Msa0255040:intron 40.0%
CGCCAGCATTAACAGATAAA+GGG + chr2_2:48655149-48655168 Msa0255040:intron 40.0%
ACTTCCTTGTGAAAAGTCAC+CGG - chr2_2:48655242-48655261 None:intergenic 40.0%
ATGCTTATGTTGGCAAGCTA+AGG + chr2_2:48655263-48655282 Msa0255040:CDS 40.0%
ATAATTTATGATGCCTCGGC+TGG + chr2_2:48655523-48655542 Msa0255040:CDS 40.0%
! CGGCTTGTTCTGTCATTTAA+TGG - chr2_2:48655556-48655575 None:intergenic 40.0%
AGTTACCAGAAGGAACTTGA+GGG - chr2_2:48655603-48655622 None:intergenic 40.0%
AAGTTACCAGAAGGAACTTG+AGG - chr2_2:48655604-48655623 None:intergenic 40.0%
TGAAGAACAAAGCTCCAATG+TGG + chr2_2:48655803-48655822 Msa0255040:CDS 40.0%
TCTAATTGCTCGTTCCACAT+TGG - chr2_2:48655820-48655839 None:intergenic 40.0%
CTTGAACTTCCATGAGAGAA+AGG + chr2_2:48655849-48655868 Msa0255040:CDS 40.0%
CTGTCATATCCTTTCTCTCA+TGG - chr2_2:48655861-48655880 None:intergenic 40.0%
!! CTCCTCCAGTTTTGTAAAGT+AGG + chr2_2:48655961-48655980 Msa0255040:CDS 40.0%
!! CCTCCAGTTTTGTAAAGTAG+GGG + chr2_2:48655963-48655982 Msa0255040:CDS 40.0%
!! CTCCAGTTTTGTAAAGTAGG+GGG + chr2_2:48655964-48655983 Msa0255040:CDS 40.0%
TAAGGGCTGTCTATAGTACA+TGG - chr2_2:48656001-48656020 None:intergenic 40.0%
! GCCATTTGCTTAACTAGCTT+CGG + chr2_2:48656039-48656058 Msa0255040:CDS 40.0%
CATGCTTCCAGAACTTCTTG+CGG + chr2_2:48653913-48653932 Msa0255040:CDS 45.0%
TTGATGTCACCGGAGAACAA+TGG + chr2_2:48653951-48653970 Msa0255040:CDS 45.0%
ATATCACACGTGAGGTCGTA+GGG + chr2_2:48654026-48654045 Msa0255040:CDS 45.0%
GTAGGGTCACCTTCTCTTAA+CGG + chr2_2:48654043-48654062 Msa0255040:CDS 45.0%
GCTGGAAGTCATCAAAACCA+AGG - chr2_2:48654890-48654909 None:intergenic 45.0%
!! GTTTTGATGACTTCCAGCCA+GGG + chr2_2:48654892-48654911 Msa0255040:intron 45.0%
TTACAACACAGTGGTGAGGA+AGG - chr2_2:48654926-48654945 None:intergenic 45.0%
ATGGAGTAAGGGCAAGATAG+AGG - chr2_2:48654977-48654996 None:intergenic 45.0%
GAGGACCTTGAGTTTAAACC+CGG + chr2_2:48655338-48655357 Msa0255040:intron 45.0%
TTCGTCCGGGTTTAAACTCA+AGG - chr2_2:48655346-48655365 None:intergenic 45.0%
TCTGTTTGCTTGCAAAACGC+CGG - chr2_2:48655576-48655595 None:intergenic 45.0%
ATAAGCCCTCAAGTTCCTTC+TGG + chr2_2:48655595-48655614 Msa0255040:CDS 45.0%
AGTTCCTTCTGGTAACTTCG+AGG + chr2_2:48655606-48655625 Msa0255040:CDS 45.0%
CTTTCGCATATTGATGAGGC+AGG - chr2_2:48655709-48655728 None:intergenic 45.0%
! TTTTCAGCTGGTTGCTACTG+TGG + chr2_2:48655897-48655916 Msa0255040:CDS 45.0%
CCCCTACTTTACAAAACTGG+AGG - chr2_2:48655966-48655985 None:intergenic 45.0%
CACCCCCTACTTTACAAAAC+TGG - chr2_2:48655969-48655988 None:intergenic 45.0%
TTAAGCAAATGGCGAACGCA+TGG - chr2_2:48656032-48656051 None:intergenic 45.0%
GCCGAAGCTAGTTAAGCAAA+TGG - chr2_2:48656043-48656062 None:intergenic 45.0%
! AACTAGCTTCGGCACTAAAC+TGG + chr2_2:48656050-48656069 Msa0255040:CDS 45.0%
!!! ATATATATTATTTTAATATC+AGG + chr2_2:48654796-48654815 Msa0255040:intron 5.0%
TGACACCACCGATAGTGCAT+TGG + chr2_2:48653868-48653887 Msa0255040:CDS 50.0%
!! CGCAAGAAGTTCTGGAAGCA+TGG - chr2_2:48653915-48653934 None:intergenic 50.0%
CTTGTGCCTGCGTTTGCAAA+CGG + chr2_2:48653996-48654015 Msa0255040:CDS 50.0%
GTGGAGAGATATCACACGTG+AGG + chr2_2:48654018-48654037 Msa0255040:CDS 50.0%
GATATCACACGTGAGGTCGT+AGG + chr2_2:48654025-48654044 Msa0255040:CDS 50.0%
! TGTCAGTGTCGTATCGGTGT+CGG + chr2_2:48654320-48654339 Msa0255040:intron 50.0%
TATGAAGCACGGACACACCT+CGG - chr2_2:48654381-48654400 None:intergenic 50.0%
TGAAGCCAAGTTTACTCGGC+TGG + chr2_2:48654579-48654598 Msa0255040:intron 50.0%
!! GGTTTTGATGACTTCCAGCC+AGG + chr2_2:48654891-48654910 Msa0255040:intron 50.0%
! GATGCCGGTGACTTTTCACA+AGG + chr2_2:48655235-48655254 Msa0255040:CDS 50.0%
CTTCTGGTAACTTCGAGGCA+GGG + chr2_2:48655611-48655630 Msa0255040:CDS 50.0%
ACCAAAACGTCCAAGGCGAA+TGG + chr2_2:48655666-48655685 Msa0255040:CDS 50.0%
! ACCATTCGCCTTGGACGTTT+TGG - chr2_2:48655670-48655689 None:intergenic 50.0%
AGAGAGCAAACCATTCGCCT+TGG - chr2_2:48655679-48655698 None:intergenic 50.0%
GGAACGAGCAATTAGAGTGC+TGG + chr2_2:48655824-48655843 Msa0255040:CDS 50.0%
TCTTCATCTCCTTTCCCTCC+TGG - chr2_2:48655934-48655953 None:intergenic 50.0%
AACGCATGGAAGGCGGATAA+GGG - chr2_2:48656018-48656037 None:intergenic 50.0%
GCTCAGGCTCTCAATGCAAC+CGG + chr2_2:48653842-48653861 Msa0255040:CDS 55.0%
!! ACTATCGGTGGTGTCATCGC+CGG - chr2_2:48653864-48653883 None:intergenic 55.0%
CACCACCGATAGTGCATTGG+AGG + chr2_2:48653871-48653890 Msa0255040:CDS 55.0%
GTCCTCCAATGCACTATCGG+TGG - chr2_2:48653876-48653895 None:intergenic 55.0%
ACGATGCGGCCATTGTTCTC+CGG - chr2_2:48653963-48653982 None:intergenic 55.0%
GCGACGACATTTCGACGATG+CGG - chr2_2:48653977-48653996 None:intergenic 55.0%
TCTCCACCGTTTGCAAACGC+AGG - chr2_2:48654005-48654024 None:intergenic 55.0%
GGTTAGACGTGTCACGGTGT+CGG - chr2_2:48654359-48654378 None:intergenic 55.0%
! ACCTCGGGTTAGACGTGTCA+CGG - chr2_2:48654365-48654384 None:intergenic 55.0%
ATGAAGCACGGACACACCTC+GGG - chr2_2:48654380-48654399 None:intergenic 55.0%
GAGGTGTGTCCGTGCTTCAT+AGG + chr2_2:48654380-48654399 Msa0255040:intron 55.0%
GTGTGTCCGTGCTTCATAGG+TGG + chr2_2:48654383-48654402 Msa0255040:intron 55.0%
GGAGTCCAGCCGAGTAAACT+TGG - chr2_2:48654587-48654606 None:intergenic 55.0%
! TAATGGTTTGGCACCAGCCG+AGG - chr2_2:48655539-48655558 None:intergenic 55.0%
CCTGCCTCGAAGTTACCAGA+AGG - chr2_2:48655613-48655632 None:intergenic 55.0%
CCTTCTGGTAACTTCGAGGC+AGG + chr2_2:48655610-48655629 Msa0255040:CDS 55.0%
ACTGTGGACCAAAGCCAACC+AGG + chr2_2:48655913-48655932 Msa0255040:CDS 55.0%
TGGACCAAAGCCAACCAGGA+GGG + chr2_2:48655917-48655936 Msa0255040:CDS 55.0%
!! CTTTCCCTCCTGGTTGGCTT+TGG - chr2_2:48655924-48655943 None:intergenic 55.0%
CAAAGCCAACCAGGAGGGAA+AGG + chr2_2:48655922-48655941 Msa0255040:CDS 55.0%
CATCTCCTTTCCCTCCTGGT+TGG - chr2_2:48655930-48655949 None:intergenic 55.0%
GAACGCATGGAAGGCGGATA+AGG - chr2_2:48656019-48656038 None:intergenic 55.0%
AATGGCGAACGCATGGAAGG+CGG - chr2_2:48656025-48656044 None:intergenic 55.0%
GCAAATGGCGAACGCATGGA+AGG - chr2_2:48656028-48656047 None:intergenic 55.0%
GTGCCTGCGTTTGCAAACGG+TGG + chr2_2:48653999-48654018 Msa0255040:CDS 60.0%
ACCGTGACACGTCTAACCCG+AGG + chr2_2:48654361-48654380 Msa0255040:intron 60.0%
GTGGACCAAAGCCAACCAGG+AGG + chr2_2:48655916-48655935 Msa0255040:CDS 60.0%
CAGTGGTGAGGAAGGTCGCG+TGG - chr2_2:48654918-48654937 None:intergenic 65.0%
TGAGGAAGGTCGCGTGGCCC+TGG - chr2_2:48654912-48654931 None:intergenic 70.0%
GAAGGTCGCGTGGCCCTGGC+TGG - chr2_2:48654908-48654927 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 48653797 48656280 48653797 ID=Msa0255040;Name=Msa0255040
chr2_2 mRNA 48653797 48656280 48653797 ID=Msa0255040-mRNA-1;Parent=Msa0255040;Name=Msa0255040-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0|0.33|0.25|1|1|1|4|197|367
chr2_2 exon 48653797 48654096 48653797 ID=Msa0255040-mRNA-1:exon:14739;Parent=Msa0255040-mRNA-1
chr2_2 exon 48654909 48654996 48654909 ID=Msa0255040-mRNA-1:exon:14740;Parent=Msa0255040-mRNA-1
chr2_2 exon 48655155 48655284 48655155 ID=Msa0255040-mRNA-1:exon:14741;Parent=Msa0255040-mRNA-1
chr2_2 exon 48655498 48656280 48655498 ID=Msa0255040-mRNA-1:exon:14742;Parent=Msa0255040-mRNA-1
chr2_2 CDS 48653797 48654096 48653797 ID=Msa0255040-mRNA-1:cds;Parent=Msa0255040-mRNA-1
chr2_2 CDS 48654909 48654996 48654909 ID=Msa0255040-mRNA-1:cds;Parent=Msa0255040-mRNA-1
chr2_2 CDS 48655155 48655284 48655155 ID=Msa0255040-mRNA-1:cds;Parent=Msa0255040-mRNA-1
chr2_2 CDS 48655498 48656083 48655498 ID=Msa0255040-mRNA-1:cds;Parent=Msa0255040-mRNA-1
chr2_2 three_prime_UTR 48656084 48656280 48656084 ID=Msa0255040-mRNA-1:three_prime_utr;Parent=Msa0255040-mRNA-1
Gene Sequence

>Msa0255040

ATGAAGCTCTTTCGATCTCTTAAATTCTTCAGATACTTCAACAATGCTCAGGCTCTCAATGCAACCGGCGATGACACCACCGATAGTGCATTGGAGGACAAAACTGATTGGTCTACCATGCTTCCAGAACTTCTTGCGGATATTATTAAACGAGTTGATGTCACCGGAGAACAATGGCCGCATCGTCGAAATGTCGTCGCTTGTGCCTGCGTTTGCAAACGGTGGAGAGATATCACACGTGAGGTCGTAGGGTCACCTTCTCTTAACGGAAAAATCACTTTCCCTTCTTGTCTTAAACAGCCAGGGCCACGCGACCTTCCTCACCACTGTGTTGTAAATTGGAATAAGAAGACCTCCACATTTTACCTCTATCTTGCCCTTACTCCATCATTAACAGATAAAGGGAAGTTCCTATTAGCAGCTAGAAGATATCGGTTTGTTACGCATACTGAGTATGTTATATCACTTGATGCCGGTGACTTTTCACAAGGAAGTAATGCTTATGTTGGCAAGCTAAGTTCAGATTTTCTTGAAGCAAGCTTCATAATTTATGATGCCTCGGCTGGTGCCAAACCATTAAATGACAGAACAAGCCGGCGTTTTGCAAGCAAACAGATAAGCCCTCAAGTTCCTTCTGGTAACTTCGAGGCAGGGAACATCTCCTACAAAAAATTCAGCCTTTTGAAACCAAAACGTCCAAGGCGAATGGTTTGCTCTCTGAAGTCTCCTGCCTCATCAATATGCGAAAGTTCAGACAGCAAGTCTTTCGATCAACATACGATGTACTGTAAAGAACAAGCTACTTCTTCTGCCTACACAATCTTGAAGAACAAAGCTCCAATGTGGAACGAGCAATTAGAGTGCTGGTTCTTGAACTTCCATGAGAGAAAGGATATGACAGTAGCATCAGTGAAGAATTTTCAGCTGGTTGCTACTGTGGACCAAAGCCAACCAGGAGGGAAAGGAGATGAAGAAACAGTTCTCCTCCAGTTTTGTAAAGTAGGGGGTGATACTTTCACCATGTACTATAGACAGCCCTTATCCGCCTTCCATGCGTTCGCCATTTGCTTAACTAGCTTCGGCACTAAACTGGCTTGTCAGTAA
Protein sequence

>Msa0255040

MKLFRSLKFFRYFNNAQALNATGDDTTDSALEDKTDWSTMLPELLADIIKRVDVTGEQWPHRRNVVACACVCKRWRDITREVVGSPSLNGKITFPSCLKQPGPRDLPHHCVVNWNKKTSTFYLYLALTPSLTDKGKFLLAARRYRFVTHTEYVISLDAGDFSQGSNAYVGKLSSDFLEASFIIYDASAGAKPLNDRTSRRFASKQISPQVPSGNFEAGNISYKKFSLLKPKRPRRMVCSLKSPASSICESSDSKSFDQHTMYCKEQATSSAYTILKNKAPMWNEQLECWFLNFHERKDMTVASVKNFQLVATVDQSQPGGKGDEETVLLQFCKVGGDTFTMYYRQPLSAFHAFAICLTSFGTKLACQ*