AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0258750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0258750 MtrunA17_Chr2g0321411 82.759 290 30 3 1 272 1 288 1.02e-169 470
Msa0258750 MtrunA17_Chr2g0321381 63.542 288 56 7 1 240 1 287 9.82e-108 314
Msa0258750 MtrunA17_Chr3g0080341 32.372 312 118 12 19 257 29 320 4.27e-26 104
Msa0258750 MtrunA17_Chr8g0355121 35.581 267 112 12 18 237 32 285 7.27e-26 103
Msa0258750 MtrunA17_Chr7g0254991 33.216 283 112 10 19 238 25 293 3.57e-24 99.4
Msa0258750 MtrunA17_Chr7g0266211 41.481 135 62 3 18 135 47 181 8.67e-23 96.7
Msa0258750 MtrunA17_Chr7g0266211 57.732 97 38 1 172 265 323 419 9.86e-21 90.9
Msa0258750 MtrunA17_Chr6g0460301 32.609 276 122 9 19 237 25 293 1.18e-17 81.3
Msa0258750 MtrunA17_Chr5g0440841 30.568 229 133 6 18 237 30 241 2.17e-17 79.7
Msa0258750 MtrunA17_Chr3g0133971 52.564 78 30 1 160 237 325 395 3.06e-15 75.1
Msa0258750 MtrunA17_Chr3g0133971 37.600 125 57 6 18 134 35 146 1.49e-13 70.1
Msa0258750 MtrunA17_Chr4g0044771 45.098 102 44 3 163 263 281 371 4.44e-13 68.6
Msa0258750 MtrunA17_Chr4g0051101 35.556 135 63 4 117 251 77 187 1.45e-12 65.1
Msa0258750 MtrunA17_Chr1g0201751 28.641 206 109 5 68 253 34 221 3.96e-11 61.6
Msa0258750 MtrunA17_Chr5g0405951 39.516 124 53 3 141 249 120 236 5.15e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0258750 AT3G44460.1 41.958 286 119 10 18 265 43 319 2.20e-48 163
Msa0258750 AT3G56850.1 34.615 260 116 11 18 237 24 269 7.19e-28 108
Msa0258750 AT2G36270.3 56.436 101 43 1 151 250 312 412 1.69e-27 110
Msa0258750 AT2G36270.3 45.333 75 28 2 71 132 145 219 1.41e-11 64.7
Msa0258750 AT2G36270.1 55.340 103 45 1 151 252 312 414 2.15e-27 110
Msa0258750 AT2G36270.1 31.638 177 57 6 18 132 45 219 7.43e-15 74.3
Msa0258750 AT2G36270.2 55.340 103 45 1 151 252 312 414 2.15e-27 110
Msa0258750 AT2G36270.2 31.638 177 57 6 18 132 45 219 7.43e-15 74.3
Msa0258750 AT2G41070.5 32.127 221 115 4 27 238 41 235 4.90e-25 100
Msa0258750 AT2G41070.3 32.127 221 115 4 27 238 41 235 1.00e-24 99.8
Msa0258750 AT2G41070.1 32.127 221 115 4 27 238 41 235 1.00e-24 99.8
Msa0258750 AT2G41070.2 32.127 221 115 4 27 238 41 235 1.00e-24 99.8
Msa0258750 AT2G41070.4 32.127 221 115 4 27 238 50 244 1.20e-24 99.8
Msa0258750 AT5G44080.1 29.787 188 99 5 81 252 120 290 1.17e-15 75.9
Msa0258750 AT1G45249.1 48.718 78 32 1 160 237 311 380 8.78e-15 73.9
Msa0258750 AT1G45249.7 48.718 78 32 1 160 237 326 395 1.06e-14 73.9
Msa0258750 AT1G45249.6 48.718 78 32 1 160 237 326 395 1.06e-14 73.9
Msa0258750 AT1G03970.1 30.168 179 101 5 81 252 85 246 3.22e-14 71.2
Msa0258750 AT4G34000.1 46.667 90 38 2 163 252 352 431 6.74e-14 71.6
Msa0258750 AT4G34000.4 46.667 90 38 2 163 252 352 431 6.74e-14 71.6
Msa0258750 AT4G34000.2 46.667 90 38 2 163 252 352 431 6.74e-14 71.6
Msa0258750 AT3G19290.1 59.016 61 25 0 190 250 348 408 1.55e-13 70.5
Msa0258750 AT3G19290.5 59.016 61 25 0 190 250 348 408 1.55e-13 70.5
Msa0258750 AT3G19290.4 56.452 62 27 0 190 251 348 409 1.67e-13 70.5
Msa0258750 AT3G19290.3 51.316 76 35 1 190 265 348 421 3.10e-13 69.7
Msa0258750 AT1G49720.1 34.351 131 71 4 26 148 47 170 2.26e-12 67.0
Msa0258750 AT1G49720.1 55.738 61 27 0 190 250 308 368 7.19e-12 65.5
Msa0258750 AT1G49720.3 34.351 131 71 4 26 148 47 170 2.26e-12 67.0
Msa0258750 AT1G49720.3 55.738 61 27 0 190 250 308 368 7.19e-12 65.5
Msa0258750 AT2G17770.2 32.143 196 107 7 84 257 2 193 2.42e-12 64.7
Msa0258750 AT1G49720.2 34.351 131 71 4 26 148 47 170 2.58e-12 66.6
Msa0258750 AT1G49720.2 53.226 62 29 0 190 251 308 369 8.65e-12 65.1
Msa0258750 AT4G34000.3 49.333 75 28 2 163 237 352 416 3.16e-12 66.6
Msa0258750 AT1G45249.3 42.697 89 32 2 160 237 311 391 6.69e-12 65.5
Msa0258750 AT4G35900.1 52.941 68 27 1 195 257 216 283 6.05e-11 62.0

Find 45 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCATCAATTATCCTCTTTC+TGG 0.164582 2_2:+55371089 None:intergenic
ATGAGTTCCTAGCCAGTATT+TGG 0.254341 2_2:-55371530 Msa0258750:CDS
TAAAATGCTGAAACAATTTC+TGG 0.256037 2_2:-55370563 Msa0258750:intron
CTTGTCATTTGGTATGTTGC+AGG 0.294849 2_2:+55371138 None:intergenic
TTTCGCTCCAATAGAACTTC+AGG 0.322405 2_2:+55371063 None:intergenic
CCACTGAATATTGCAAGTTC+TGG 0.324558 2_2:-55371177 Msa0258750:CDS
ACTCAGCTCCACTTGTCATT+TGG 0.334133 2_2:+55371127 None:intergenic
AGAAAACTTCGGATGAAGTT+TGG 0.353717 2_2:-55371389 Msa0258750:CDS
CAAAGTTGTTGAAATCATCT+AGG 0.405368 2_2:+55371568 None:intergenic
GCAATCAGCAAAGGTTCAAA+AGG 0.420453 2_2:-55370312 Msa0258750:CDS
GACTGAATCACCAAGTTGTT+GGG 0.423167 2_2:+55371434 None:intergenic
ATCACTGGAACTCCAAATAC+TGG 0.430426 2_2:+55371518 None:intergenic
TTTCTTCTTTGAATTGTGGT+TGG 0.440685 2_2:+55371349 None:intergenic
GAGGATTTCTTGGTAAAAGC+TGG 0.453104 2_2:-55371267 Msa0258750:CDS
GTCATTGAGGCTTATTCACC+AGG 0.470334 2_2:+55370262 None:intergenic
AGACTGAATCACCAAGTTGT+TGG 0.471123 2_2:+55371433 None:intergenic
ATCACCAAGTTGTTGGGAAC+TGG 0.493001 2_2:+55371440 None:intergenic
CACACCAGTTCCCAACAACT+TGG 0.498257 2_2:-55371444 Msa0258750:CDS
TCCTCAAAAGTCATTTCTCC+AGG 0.507331 2_2:+55371285 None:intergenic
CAATCAGCAAAGGTTCAAAA+GGG 0.509524 2_2:-55370311 Msa0258750:CDS
TTCGCTCCAATAGAACTTCA+GGG 0.513014 2_2:+55371064 None:intergenic
TAGGAACTCATCCATGTTGA+GGG 0.517404 2_2:+55371542 None:intergenic
TTGTGTAGTAGCTTCATCAC+TGG 0.520796 2_2:+55371503 None:intergenic
ACGTTCTCGAGCAAGACGTC+AGG 0.524988 2_2:-55371004 Msa0258750:intron
ACCAGAAAGAGGATAATTGA+TGG 0.532983 2_2:-55371090 Msa0258750:CDS
TCCAGTTTGCAAGAAAACTT+CGG 0.535572 2_2:-55371400 Msa0258750:CDS
AAAGTTGTTGAAATCATCTA+GGG 0.545896 2_2:+55371569 None:intergenic
GCAAAGAAAGCAATCAGCAA+AGG 0.559335 2_2:-55370321 Msa0258750:CDS
TTGTCGTTTCAGAATCATAG+TGG 0.559475 2_2:-55371222 Msa0258750:CDS
CGCTCCAATAGAACTTCAGG+GGG 0.565451 2_2:+55371066 None:intergenic
CTAGGAACTCATCCATGTTG+AGG 0.566833 2_2:+55371541 None:intergenic
CAACTTTGACTCCCTCAACA+TGG 0.568231 2_2:-55371553 Msa0258750:CDS
CTCAAGAAGCAAGAAACACC+TGG 0.571531 2_2:-55371303 Msa0258750:CDS
AGGCTGAAGCTGAGAACAAG+AGG 0.580689 2_2:-55370443 Msa0258750:CDS
TGGAACTCCAAATACTGGCT+AGG 0.584202 2_2:+55371523 None:intergenic
TCTACAACAATTAGATCAAG+AGG 0.584508 2_2:-55371622 Msa0258750:CDS
AATCAAGCTCAAGCACAACC+TGG 0.585556 2_2:-55370280 Msa0258750:CDS
GTGGAGCTGCTACCAGAAAG+AGG 0.587980 2_2:-55371101 Msa0258750:CDS
GGCTTATACAATAGAGCTAG+AGG 0.593351 2_2:-55370614 Msa0258750:CDS
GCAACATACCAAATGACAAG+TGG 0.610150 2_2:-55371135 Msa0258750:CDS
ACAAGTGGAGCTGAGTCAAG+TGG 0.615825 2_2:-55371120 Msa0258750:CDS
CCAGAACTTGCAATATTCAG+TGG 0.629052 2_2:+55371177 None:intergenic
TCGCTCCAATAGAACTTCAG+GGG 0.629189 2_2:+55371065 None:intergenic
TGAGAACAAGAGGAAACAAG+AGG 0.643173 2_2:-55370433 Msa0258750:intron
AATCAGCAAAGGTTCAAAAG+GGG 0.678708 2_2:-55370310 Msa0258750:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTCTTCTTTGAATTG+TGG + chr2_2:55370562-55370581 None:intergenic 20.0%
!! TACTTATTAATCACATAACT+TGG - chr2_2:55370968-55370987 Msa0258750:intron 20.0%
!!! TTTTTTATATGCAAACGTTA+CGG + chr2_2:55371255-55371274 None:intergenic 20.0%
!!! TACCTATTTAAATCTGAAAT+AGG - chr2_2:55371441-55371460 Msa0258750:CDS 20.0%
!!! TTATTTTCTTCTTTGAATTG+TGG + chr2_2:55370562-55370581 None:intergenic 20.0%
!! TACTTATTAATCACATAACT+TGG - chr2_2:55370968-55370987 Msa0258750:intron 20.0%
!!! TTTTTTATATGCAAACGTTA+CGG + chr2_2:55371255-55371274 None:intergenic 20.0%
!!! TACCTATTTAAATCTGAAAT+AGG - chr2_2:55371441-55371460 Msa0258750:CDS 20.0%
! AAAGTTGTTGAAATCATCTA+GGG + chr2_2:55370338-55370357 None:intergenic 25.0%
! AATGATGAAAAATCGAGAAT+CGG - chr2_2:55370873-55370892 Msa0258750:intron 25.0%
!! AGATTTTAAATAACAGTCTG+TGG - chr2_2:55371150-55371169 Msa0258750:CDS 25.0%
!!! TATGTCTCTGAAATTAAAGT+TGG - chr2_2:55371204-55371223 Msa0258750:CDS 25.0%
!!! ATGTCTCTGAAATTAAAGTT+GGG - chr2_2:55371205-55371224 Msa0258750:CDS 25.0%
! AAAAAAAAATCTTGCAATGC+AGG - chr2_2:55371269-55371288 Msa0258750:CDS 25.0%
! TAAAATGCTGAAACAATTTC+TGG - chr2_2:55371341-55371360 Msa0258750:CDS 25.0%
! CAATACTGAAACTGAATATA+CGG - chr2_2:55371368-55371387 Msa0258750:CDS 25.0%
! AGCCTATTTCAGATTTAAAT+AGG + chr2_2:55371446-55371465 None:intergenic 25.0%
! AAAGTTGTTGAAATCATCTA+GGG + chr2_2:55370338-55370357 None:intergenic 25.0%
! AATGATGAAAAATCGAGAAT+CGG - chr2_2:55370873-55370892 Msa0258750:intron 25.0%
!! AGATTTTAAATAACAGTCTG+TGG - chr2_2:55371150-55371169 Msa0258750:CDS 25.0%
!!! TATGTCTCTGAAATTAAAGT+TGG - chr2_2:55371204-55371223 Msa0258750:CDS 25.0%
!!! ATGTCTCTGAAATTAAAGTT+GGG - chr2_2:55371205-55371224 Msa0258750:CDS 25.0%
! AAAAAAAAATCTTGCAATGC+AGG - chr2_2:55371269-55371288 Msa0258750:CDS 25.0%
! TAAAATGCTGAAACAATTTC+TGG - chr2_2:55371341-55371360 Msa0258750:CDS 25.0%
! CAATACTGAAACTGAATATA+CGG - chr2_2:55371368-55371387 Msa0258750:CDS 25.0%
! AGCCTATTTCAGATTTAAAT+AGG + chr2_2:55371446-55371465 None:intergenic 25.0%
TCTACAACAATTAGATCAAG+AGG - chr2_2:55370282-55370301 Msa0258750:CDS 30.0%
CAAAGTTGTTGAAATCATCT+AGG + chr2_2:55370339-55370358 None:intergenic 30.0%
!!! TGGTTGGTTTTTATGAATCT+CGG + chr2_2:55370542-55370561 None:intergenic 30.0%
TTTCTTCTTTGAATTGTGGT+TGG + chr2_2:55370558-55370577 None:intergenic 30.0%
! TGAGTGTCTCATTTCTTTTA+AGG + chr2_2:55370585-55370604 None:intergenic 30.0%
!! AATGACTTTTGAGGATTTCT+TGG - chr2_2:55370627-55370646 Msa0258750:intron 30.0%
TAAAATCGTGTTTACCTTTG+CGG - chr2_2:55371012-55371031 Msa0258750:CDS 30.0%
TCTACAACAATTAGATCAAG+AGG - chr2_2:55370282-55370301 Msa0258750:CDS 30.0%
CAAAGTTGTTGAAATCATCT+AGG + chr2_2:55370339-55370358 None:intergenic 30.0%
!!! TGGTTGGTTTTTATGAATCT+CGG + chr2_2:55370542-55370561 None:intergenic 30.0%
TTTCTTCTTTGAATTGTGGT+TGG + chr2_2:55370558-55370577 None:intergenic 30.0%
! TGAGTGTCTCATTTCTTTTA+AGG + chr2_2:55370585-55370604 None:intergenic 30.0%
!! AATGACTTTTGAGGATTTCT+TGG - chr2_2:55370627-55370646 Msa0258750:intron 30.0%
TAAAATCGTGTTTACCTTTG+CGG - chr2_2:55371012-55371031 Msa0258750:CDS 30.0%
TCCAGTTTGCAAGAAAACTT+CGG - chr2_2:55370504-55370523 Msa0258750:intron 35.0%
AGAAAACTTCGGATGAAGTT+TGG - chr2_2:55370515-55370534 Msa0258750:intron 35.0%
! TTGTCGTTTCAGAATCATAG+TGG - chr2_2:55370682-55370701 Msa0258750:intron 35.0%
ACCAGAAAGAGGATAATTGA+TGG - chr2_2:55370814-55370833 Msa0258750:intron 35.0%
ACCATCAATTATCCTCTTTC+TGG + chr2_2:55370818-55370837 None:intergenic 35.0%
TTGGATTTATCGCGAAATCA+AGG + chr2_2:55371050-55371069 None:intergenic 35.0%
AAATCCAACACAAATGTGAC+AGG - chr2_2:55371062-55371081 Msa0258750:CDS 35.0%
TTATGTCACAGCCTAAATCA+CGG + chr2_2:55371176-55371195 None:intergenic 35.0%
!! CTCTGAAATTAAAGTTGGGA+CGG - chr2_2:55371209-55371228 Msa0258750:CDS 35.0%
CAATCAGCAAAGGTTCAAAA+GGG - chr2_2:55371593-55371612 Msa0258750:CDS 35.0%
AATCAGCAAAGGTTCAAAAG+GGG - chr2_2:55371594-55371613 Msa0258750:CDS 35.0%
TCCAGTTTGCAAGAAAACTT+CGG - chr2_2:55370504-55370523 Msa0258750:intron 35.0%
AGAAAACTTCGGATGAAGTT+TGG - chr2_2:55370515-55370534 Msa0258750:intron 35.0%
! TTGTCGTTTCAGAATCATAG+TGG - chr2_2:55370682-55370701 Msa0258750:intron 35.0%
ACCAGAAAGAGGATAATTGA+TGG - chr2_2:55370814-55370833 Msa0258750:intron 35.0%
ACCATCAATTATCCTCTTTC+TGG + chr2_2:55370818-55370837 None:intergenic 35.0%
TTGGATTTATCGCGAAATCA+AGG + chr2_2:55371050-55371069 None:intergenic 35.0%
AAATCCAACACAAATGTGAC+AGG - chr2_2:55371062-55371081 Msa0258750:CDS 35.0%
TTATGTCACAGCCTAAATCA+CGG + chr2_2:55371176-55371195 None:intergenic 35.0%
!! CTCTGAAATTAAAGTTGGGA+CGG - chr2_2:55371209-55371228 Msa0258750:CDS 35.0%
CAATCAGCAAAGGTTCAAAA+GGG - chr2_2:55371593-55371612 Msa0258750:CDS 35.0%
AATCAGCAAAGGTTCAAAAG+GGG - chr2_2:55371594-55371613 Msa0258750:CDS 35.0%
!!! GTAAGTGCGTTTTGATTGCT+CGG + chr2_2:55370316-55370335 None:intergenic 40.0%
TAGGAACTCATCCATGTTGA+GGG + chr2_2:55370365-55370384 None:intergenic 40.0%
ATGAGTTCCTAGCCAGTATT+TGG - chr2_2:55370374-55370393 Msa0258750:intron 40.0%
ATCACTGGAACTCCAAATAC+TGG + chr2_2:55370389-55370408 None:intergenic 40.0%
TTGTGTAGTAGCTTCATCAC+TGG + chr2_2:55370404-55370423 None:intergenic 40.0%
GACTGAATCACCAAGTTGTT+GGG + chr2_2:55370473-55370492 None:intergenic 40.0%
AGACTGAATCACCAAGTTGT+TGG + chr2_2:55370474-55370493 None:intergenic 40.0%
! TTTTCTTGCAAACTGGAGGA+GGG + chr2_2:55370501-55370520 None:intergenic 40.0%
! GAAGTTTTCTTGCAAACTGG+AGG + chr2_2:55370505-55370524 None:intergenic 40.0%
! TCCGAAGTTTTCTTGCAAAC+TGG + chr2_2:55370508-55370527 None:intergenic 40.0%
! ACCTGGAGAAATGACTTTTG+AGG - chr2_2:55370618-55370637 Msa0258750:intron 40.0%
TCCTCAAAAGTCATTTCTCC+AGG + chr2_2:55370622-55370641 None:intergenic 40.0%
GAGGATTTCTTGGTAAAAGC+TGG - chr2_2:55370637-55370656 Msa0258750:intron 40.0%
CCAGAACTTGCAATATTCAG+TGG + chr2_2:55370730-55370749 None:intergenic 40.0%
CCACTGAATATTGCAAGTTC+TGG - chr2_2:55370727-55370746 Msa0258750:intron 40.0%
! CTTGTCATTTGGTATGTTGC+AGG + chr2_2:55370769-55370788 None:intergenic 40.0%
GCAACATACCAAATGACAAG+TGG - chr2_2:55370769-55370788 Msa0258750:intron 40.0%
TTCGCTCCAATAGAACTTCA+GGG + chr2_2:55370843-55370862 None:intergenic 40.0%
TTTCGCTCCAATAGAACTTC+AGG + chr2_2:55370844-55370863 None:intergenic 40.0%
TGAACCTGTCACATTTGTGT+TGG + chr2_2:55371069-55371088 None:intergenic 40.0%
GTGATTTAGGCTGTGACATA+AGG - chr2_2:55371175-55371194 Msa0258750:CDS 40.0%
GGCTTATACAATAGAGCTAG+AGG - chr2_2:55371290-55371309 Msa0258750:CDS 40.0%
TGAGAACAAGAGGAAACAAG+AGG - chr2_2:55371471-55371490 Msa0258750:CDS 40.0%
GCAAAGAAAGCAATCAGCAA+AGG - chr2_2:55371583-55371602 Msa0258750:CDS 40.0%
GCAATCAGCAAAGGTTCAAA+AGG - chr2_2:55371592-55371611 Msa0258750:CDS 40.0%
!!! GTAAGTGCGTTTTGATTGCT+CGG + chr2_2:55370316-55370335 None:intergenic 40.0%
TAGGAACTCATCCATGTTGA+GGG + chr2_2:55370365-55370384 None:intergenic 40.0%
ATGAGTTCCTAGCCAGTATT+TGG - chr2_2:55370374-55370393 Msa0258750:intron 40.0%
ATCACTGGAACTCCAAATAC+TGG + chr2_2:55370389-55370408 None:intergenic 40.0%
TTGTGTAGTAGCTTCATCAC+TGG + chr2_2:55370404-55370423 None:intergenic 40.0%
GACTGAATCACCAAGTTGTT+GGG + chr2_2:55370473-55370492 None:intergenic 40.0%
AGACTGAATCACCAAGTTGT+TGG + chr2_2:55370474-55370493 None:intergenic 40.0%
! TTTTCTTGCAAACTGGAGGA+GGG + chr2_2:55370501-55370520 None:intergenic 40.0%
! GAAGTTTTCTTGCAAACTGG+AGG + chr2_2:55370505-55370524 None:intergenic 40.0%
! TCCGAAGTTTTCTTGCAAAC+TGG + chr2_2:55370508-55370527 None:intergenic 40.0%
! ACCTGGAGAAATGACTTTTG+AGG - chr2_2:55370618-55370637 Msa0258750:intron 40.0%
TCCTCAAAAGTCATTTCTCC+AGG + chr2_2:55370622-55370641 None:intergenic 40.0%
GAGGATTTCTTGGTAAAAGC+TGG - chr2_2:55370637-55370656 Msa0258750:intron 40.0%
CCAGAACTTGCAATATTCAG+TGG + chr2_2:55370730-55370749 None:intergenic 40.0%
CCACTGAATATTGCAAGTTC+TGG - chr2_2:55370727-55370746 Msa0258750:intron 40.0%
! CTTGTCATTTGGTATGTTGC+AGG + chr2_2:55370769-55370788 None:intergenic 40.0%
GCAACATACCAAATGACAAG+TGG - chr2_2:55370769-55370788 Msa0258750:intron 40.0%
TTCGCTCCAATAGAACTTCA+GGG + chr2_2:55370843-55370862 None:intergenic 40.0%
TTTCGCTCCAATAGAACTTC+AGG + chr2_2:55370844-55370863 None:intergenic 40.0%
TGAACCTGTCACATTTGTGT+TGG + chr2_2:55371069-55371088 None:intergenic 40.0%
GTGATTTAGGCTGTGACATA+AGG - chr2_2:55371175-55371194 Msa0258750:CDS 40.0%
GGCTTATACAATAGAGCTAG+AGG - chr2_2:55371290-55371309 Msa0258750:CDS 40.0%
TGAGAACAAGAGGAAACAAG+AGG - chr2_2:55371471-55371490 Msa0258750:CDS 40.0%
GCAAAGAAAGCAATCAGCAA+AGG - chr2_2:55371583-55371602 Msa0258750:CDS 40.0%
GCAATCAGCAAAGGTTCAAA+AGG - chr2_2:55371592-55371611 Msa0258750:CDS 40.0%
CAACTTTGACTCCCTCAACA+TGG - chr2_2:55370351-55370370 Msa0258750:intron 45.0%
CTAGGAACTCATCCATGTTG+AGG + chr2_2:55370366-55370385 None:intergenic 45.0%
! TGGAACTCCAAATACTGGCT+AGG + chr2_2:55370384-55370403 None:intergenic 45.0%
ATCACCAAGTTGTTGGGAAC+TGG + chr2_2:55370467-55370486 None:intergenic 45.0%
! GTTTTCTTGCAAACTGGAGG+AGG + chr2_2:55370502-55370521 None:intergenic 45.0%
CTCAAGAAGCAAGAAACACC+TGG - chr2_2:55370601-55370620 Msa0258750:CDS 45.0%
ACTCAGCTCCACTTGTCATT+TGG + chr2_2:55370780-55370799 None:intergenic 45.0%
TCGCTCCAATAGAACTTCAG+GGG + chr2_2:55370842-55370861 None:intergenic 45.0%
! TTGGAGAGTGCATCTGTGTA+AGG - chr2_2:55370987-55371006 Msa0258750:intron 45.0%
AAAGTCTGTGACCGTGATTG+AGG + chr2_2:55371093-55371112 None:intergenic 45.0%
! GCAAACGTTACGGACTATCA+CGG + chr2_2:55371245-55371264 None:intergenic 45.0%
AATCAAGCTCAAGCACAACC+TGG - chr2_2:55371624-55371643 Msa0258750:CDS 45.0%
CAACTTTGACTCCCTCAACA+TGG - chr2_2:55370351-55370370 Msa0258750:intron 45.0%
CTAGGAACTCATCCATGTTG+AGG + chr2_2:55370366-55370385 None:intergenic 45.0%
! TGGAACTCCAAATACTGGCT+AGG + chr2_2:55370384-55370403 None:intergenic 45.0%
ATCACCAAGTTGTTGGGAAC+TGG + chr2_2:55370467-55370486 None:intergenic 45.0%
! GTTTTCTTGCAAACTGGAGG+AGG + chr2_2:55370502-55370521 None:intergenic 45.0%
CTCAAGAAGCAAGAAACACC+TGG - chr2_2:55370601-55370620 Msa0258750:CDS 45.0%
ACTCAGCTCCACTTGTCATT+TGG + chr2_2:55370780-55370799 None:intergenic 45.0%
TCGCTCCAATAGAACTTCAG+GGG + chr2_2:55370842-55370861 None:intergenic 45.0%
! TTGGAGAGTGCATCTGTGTA+AGG - chr2_2:55370987-55371006 Msa0258750:intron 45.0%
AAAGTCTGTGACCGTGATTG+AGG + chr2_2:55371093-55371112 None:intergenic 45.0%
! GCAAACGTTACGGACTATCA+CGG + chr2_2:55371245-55371264 None:intergenic 45.0%
AATCAAGCTCAAGCACAACC+TGG - chr2_2:55371624-55371643 Msa0258750:CDS 45.0%
CACACCAGTTCCCAACAACT+TGG - chr2_2:55370460-55370479 Msa0258750:intron 50.0%
ACAAGTGGAGCTGAGTCAAG+TGG - chr2_2:55370784-55370803 Msa0258750:intron 50.0%
TGGTCCCCCTGAAGTTCTAT+TGG - chr2_2:55370834-55370853 Msa0258750:intron 50.0%
CGCTCCAATAGAACTTCAGG+GGG + chr2_2:55370841-55370860 None:intergenic 50.0%
GACAGGTTCAGCCTCAATCA+CGG - chr2_2:55371079-55371098 Msa0258750:CDS 50.0%
ACAGTCTGTGGCCGTGATTT+AGG - chr2_2:55371162-55371181 Msa0258750:CDS 50.0%
AGGCTGAAGCTGAGAACAAG+AGG - chr2_2:55371461-55371480 Msa0258750:CDS 50.0%
CACACCAGTTCCCAACAACT+TGG - chr2_2:55370460-55370479 Msa0258750:intron 50.0%
ACAAGTGGAGCTGAGTCAAG+TGG - chr2_2:55370784-55370803 Msa0258750:intron 50.0%
TGGTCCCCCTGAAGTTCTAT+TGG - chr2_2:55370834-55370853 Msa0258750:intron 50.0%
CGCTCCAATAGAACTTCAGG+GGG + chr2_2:55370841-55370860 None:intergenic 50.0%
GACAGGTTCAGCCTCAATCA+CGG - chr2_2:55371079-55371098 Msa0258750:CDS 50.0%
ACAGTCTGTGGCCGTGATTT+AGG - chr2_2:55371162-55371181 Msa0258750:CDS 50.0%
AGGCTGAAGCTGAGAACAAG+AGG - chr2_2:55371461-55371480 Msa0258750:CDS 50.0%
GTGGAGCTGCTACCAGAAAG+AGG - chr2_2:55370803-55370822 Msa0258750:intron 55.0%
ACGTTCTCGAGCAAGACGTC+AGG - chr2_2:55370900-55370919 Msa0258750:intron 55.0%
GGATTGTGAAGCGACCGCAA+AGG + chr2_2:55371029-55371048 None:intergenic 55.0%
GTGGAGCTGCTACCAGAAAG+AGG - chr2_2:55370803-55370822 Msa0258750:intron 55.0%
ACGTTCTCGAGCAAGACGTC+AGG - chr2_2:55370900-55370919 Msa0258750:intron 55.0%
GGATTGTGAAGCGACCGCAA+AGG + chr2_2:55371029-55371048 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 55370277 55371649 55370277 ID=Msa0258750;Name=Msa0258750
chr2_2 mRNA 55370277 55371649 55370277 ID=Msa0258750-mRNA-1;Parent=Msa0258750;Name=Msa0258750-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0|0|0|0.75|1|1|4|0|272
chr2_2 exon 55371005 55371649 55371005 ID=Msa0258750-mRNA-1:exon:16730;Parent=Msa0258750-mRNA-1
chr2_2 exon 55370564 55370635 55370564 ID=Msa0258750-mRNA-1:exon:16729;Parent=Msa0258750-mRNA-1
chr2_2 exon 55370434 55370463 55370434 ID=Msa0258750-mRNA-1:exon:16728;Parent=Msa0258750-mRNA-1
chr2_2 exon 55370277 55370348 55370277 ID=Msa0258750-mRNA-1:exon:16727;Parent=Msa0258750-mRNA-1
chr2_2 CDS 55371005 55371649 55371005 ID=Msa0258750-mRNA-1:cds;Parent=Msa0258750-mRNA-1
chr2_2 CDS 55370564 55370635 55370564 ID=Msa0258750-mRNA-1:cds;Parent=Msa0258750-mRNA-1
chr2_2 CDS 55370434 55370463 55370434 ID=Msa0258750-mRNA-1:cds;Parent=Msa0258750-mRNA-1
chr2_2 CDS 55370277 55370348 55370277 ID=Msa0258750-mRNA-1:cds;Parent=Msa0258750-mRNA-1
Gene Sequence

>Msa0258750

ATGGCTCTACAACAATTAGATCAAGAGGATTCTCATCCGAGCAATCAAAACGCACTTACCCTAGATGATTTCAACAACTTTGACTCCCTCAACATGGATGAGTTCCTAGCCAGTATTTGGAGTTCCAGTGATGAAGCTACTACACAAACTCACAACAATATTGAAAGTGTTGTAACAACAGAACACACCAGTTCCCAACAACTTGGTGATTCAGTCTCTGTCCCTCCTCCAGTTTGCAAGAAAACTTCGGATGAAGTTTGGTCCGAGATTCATAAAAACCAACCACAATTCAAAGAAGAAAATAACCTTAAAAGAAATGAGACACTCAAGAAGCAAGAAACACCTGGAGAAATGACTTTTGAGGATTTCTTGGTAAAAGCTGGAGTAGTACAACAATCATCATCATTGTCGTTTCAGAATCATAGTGGCAATGTTTCAAATAACATGAGACCACTGAATATTGCAAGTTCTGGTTTGACTAGCAATAGTCCTGCAACATACCAAATGACAAGTGGAGCTGAGTCAAGTGGAGCTGCTACCAGAAAGAGGATAATTGATGGTCCCCCTGAAGTTCTATTGGAGCGAAAACAACGCAGAATGATGAAAAATCGAGAATCGGCTGCACGTTCTCGAGCAAGACGTCAGGCTTATACAATAGAGCTAGAGGCTGAGCTGAATCTGCTACAAGAAGAGAATAAAATGCTGAAACAATTTCTGGCTGAAGCTGAGAACAAGAGGAAACAAGAGCTTTTGCAAAGAAAGCAATCAGCAAAGGTTCAAAAGGGGACAGAAAAATCAAGCTCAAGCACAACCTGGTGA
Protein sequence

>Msa0258750

MALQQLDQEDSHPSNQNALTLDDFNNFDSLNMDEFLASIWSSSDEATTQTHNNIESVVTTEHTSSQQLGDSVSVPPPVCKKTSDEVWSEIHKNQPQFKEENNLKRNETLKKQETPGEMTFEDFLVKAGVVQQSSSLSFQNHSGNVSNNMRPLNIASSGLTSNSPATYQMTSGAESSGAATRKRIIDGPPEVLLERKQRRMMKNRESAARSRARRQAYTIELEAELNLLQEENKMLKQFLAEAENKRKQELLQRKQSAKVQKGTEKSSSSTTW*