AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0262740

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0262740 MtrunA17_Chr2g0325521 98.381 247 4 0 1 247 1 247 8.15e-180 493
Msa0262740 MtrunA17_Chr4g0041051 72.656 256 61 3 1 247 1 256 3.18e-122 348
Msa0262740 MtrunA17_Chr4g0045901 44.262 244 125 6 2 235 13 255 6.84e-50 164
Msa0262740 MtrunA17_Chr7g0255961 47.253 182 86 4 54 234 70 242 4.44e-42 144
Msa0262740 MtrunA17_Chr1g0198801 40.594 202 109 3 45 242 144 338 3.03e-34 125
Msa0262740 MtrunA17_Chr3g0113721 33.333 201 115 5 46 234 26 219 5.60e-31 114
Msa0262740 MtrunA17_Chr5g0412371 38.462 143 74 3 54 188 40 176 2.56e-26 101
Msa0262740 MtrunA17_Chr5g0427361 40.606 165 72 2 49 188 27 190 1.55e-24 98.2
Msa0262740 MtrunA17_Chr2g0283714 51.250 80 36 1 91 170 16 92 1.56e-19 81.6
Msa0262740 MtrunA17_Chr5g0411341 40.244 82 49 0 28 109 459 540 3.36e-12 65.9
Msa0262740 MtrunA17_Chr8g0366751 39.506 81 49 0 29 109 429 509 7.89e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0262740 AT1G68810.1 41.327 196 107 5 45 236 160 351 2.95e-33 124
Msa0262740 AT3G25710.1 46.622 148 70 2 45 188 118 260 4.85e-31 117
Msa0262740 AT3G56770.1 38.857 175 98 4 51 223 37 204 1.74e-30 113
Msa0262740 AT2G40200.1 39.634 164 89 3 61 219 65 223 1.23e-29 112
Msa0262740 AT2G41130.1 40.571 175 96 3 51 223 59 227 4.78e-27 105
Msa0262740 AT4G38070.1 36.154 130 76 2 59 188 92 214 9.82e-18 80.1
Msa0262740 AT1G32640.1 37.624 101 60 2 33 130 421 521 1.12e-11 64.7
Msa0262740 AT5G46760.1 44.156 77 42 1 39 114 391 467 1.97e-11 63.9
Msa0262740 AT4G17880.1 40.789 76 45 0 39 114 393 468 2.20e-11 63.9

Find 64 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATCTTCTTCACTGGTTT+TGG 0.180837 2_2:-61778204 Msa0262740:CDS
CACTTCATCTTATGATAATA+AGG 0.194458 2_2:-61776940 Msa0262740:CDS
ATTGAATGATGAAAAGAAAA+AGG 0.226203 2_2:+61778443 None:intergenic
AATGATGCTCTAATTGAATA+AGG 0.233216 2_2:+61777016 None:intergenic
TGTTGGGGTTGGAGTGTATT+TGG 0.233217 2_2:+61778484 None:intergenic
CTTATCTCATCACTGTCTTT+TGG 0.234136 2_2:+61777073 None:intergenic
ATATTCAGTTCTTCATCTTT+AGG 0.245592 2_2:-61776725 Msa0262740:CDS
CCAAGAGGGTGTTCAGAATA+TGG 0.263756 2_2:+61778319 None:intergenic
TCACAAGAAGGTGGATTAAA+TGG 0.279597 2_2:-61777043 Msa0262740:CDS
TTCACATGTAATGTGAATCT+TGG 0.292197 2_2:-61776670 Msa0262740:three_prime_UTR
TTTGCTGTTGATGCTTCTTC+TGG 0.299468 2_2:+61778583 None:intergenic
TGTTATTCCTGGTGCTAATA+AGG 0.307010 2_2:-61778030 Msa0262740:intron
GTTGGACAAAGCATCCTTAC+TGG 0.310877 2_2:-61777164 Msa0262740:CDS
TGTTGTTAATTTGTCATGTT+GGG 0.312268 2_2:+61778468 None:intergenic
ACAATGAATGAACTCATTCT+AGG 0.319427 2_2:-61776566 Msa0262740:three_prime_UTR
TGTGGAGGCAGCTGTGAAAT+TGG 0.360925 2_2:-61778162 Msa0262740:CDS
AAGGGCAGAGATTGCAACTT+TGG 0.372459 2_2:-61776921 Msa0262740:CDS
GTAAAGATTACTTCTTATAG+TGG 0.377802 2_2:+61778605 None:intergenic
TATGATGATGATAGTGAGTT+TGG 0.389810 2_2:+61778527 None:intergenic
TTGTTGTTAATTTGTCATGT+TGG 0.399163 2_2:+61778467 None:intergenic
GAGTATCGACAGTTTCTTGC+TGG 0.401116 2_2:-61776836 Msa0262740:CDS
GGTGTAAATGGAAGTGGATA+TGG 0.413506 2_2:-61778227 Msa0262740:CDS
AGGGCAGAGATTGCAACTTT+GGG 0.424505 2_2:-61776920 Msa0262740:CDS
TCTTATATCAGACAATAAAC+CGG 0.424693 2_2:+61776976 None:intergenic
CCATATTCTGAACACCCTCT+TGG 0.428144 2_2:-61778319 Msa0262740:five_prime_UTR
ATATGGTTCATCTTCTTCAC+TGG 0.433898 2_2:-61778210 Msa0262740:CDS
ACACTTCGTAGTGTTATTCC+TGG 0.436064 2_2:-61778041 Msa0262740:CDS
TTAATTTGTCATGTTGGGGT+TGG 0.442961 2_2:+61778473 None:intergenic
TGTAATAACTTCAGCCAGTA+AGG 0.442964 2_2:+61777150 None:intergenic
ATGATGCTCTAATTGAATAA+GGG 0.448376 2_2:+61777017 None:intergenic
ATCTTGGGTTGTACATTTGT+TGG 0.451003 2_2:-61776654 Msa0262740:three_prime_UTR
TTTCAAGGTGTAAATGGAAG+TGG 0.455677 2_2:-61778233 Msa0262740:CDS
TCTTGGGTTGTACATTTGTT+GGG 0.456987 2_2:-61776653 Msa0262740:three_prime_UTR
CATATTCTGAACACCCTCTT+GGG 0.457908 2_2:-61778318 Msa0262740:five_prime_UTR
AGCACTACTCTCTCTGCCTC+TGG 0.475314 2_2:-61778556 Msa0262740:five_prime_UTR
TCACATGTAATGTGAATCTT+GGG 0.483047 2_2:-61776669 Msa0262740:three_prime_UTR
TAAAGATTACTTCTTATAGT+GGG 0.485015 2_2:+61778606 None:intergenic
ACTTCATCTTATGATAATAA+GGG 0.489015 2_2:-61776939 Msa0262740:CDS
TCGACAGTTTCTTGCTGGAT+CGG 0.489881 2_2:-61776831 Msa0262740:CDS
CTTATATCAGACAATAAACC+GGG 0.504302 2_2:+61776977 None:intergenic
AAAGCTTCAATACTTCTCTC+AGG 0.509056 2_2:+61778122 None:intergenic
AAGACAACGTTGGAAGAAGA+AGG 0.529141 2_2:+61778364 None:intergenic
AGCAAGAAACTGTCGATACT+CGG 0.542573 2_2:+61776838 None:intergenic
GTTGTTAATTTGTCATGTTG+GGG 0.557500 2_2:+61778469 None:intergenic
TTATGTTGTGAATACAAACC+CGG 0.569012 2_2:-61776995 Msa0262740:CDS
GTGAGTTTGGTGAGATCCAG+AGG 0.570542 2_2:+61778540 None:intergenic
AAAGATTACTTCTTATAGTG+GGG 0.583347 2_2:+61778607 None:intergenic
GCTTCAAGTAGAATGAATGA+TGG 0.589941 2_2:-61778260 Msa0262740:CDS
GCAGAGATTGCAACTTTGGG+AGG 0.592208 2_2:-61776917 Msa0262740:CDS
GAAGAAGGATGAGGAGACAA+AGG 0.595640 2_2:+61778379 None:intergenic
ATCATAGTGAAGCAGAGAGG+AGG 0.603979 2_2:-61778094 Msa0262740:CDS
GAACTAACCTTATTAGCACC+AGG 0.604108 2_2:+61778023 None:intergenic
TCTTAGAAACTAGAAAGAGA+AGG 0.613686 2_2:-61776757 Msa0262740:CDS
TAGAAACTAGAAAGAGAAGG+AGG 0.614835 2_2:-61776754 Msa0262740:CDS
AAGATTACTTCTTATAGTGG+GGG 0.618050 2_2:+61778608 None:intergenic
ATAAGAGTTGAATCACAAGA+AGG 0.621854 2_2:-61777055 Msa0262740:CDS
AGAGTTGAATCACAAGAAGG+TGG 0.624818 2_2:-61777052 Msa0262740:CDS
AATGCAGCACAAGCAAGTGA+AGG 0.627293 2_2:-61777106 Msa0262740:CDS
GACACAAAGAAAGACAACGT+TGG 0.650206 2_2:+61778354 None:intergenic
AAACAACACAGTTCCCAAGA+GGG 0.661371 2_2:+61778305 None:intergenic
ACGTTGGAAGAAGAAGGATG+AGG 0.668662 2_2:+61778370 None:intergenic
AAAACAACACAGTTCCCAAG+AGG 0.688172 2_2:+61778304 None:intergenic
GCTGTGAAATTGGAACGCAA+AGG 0.704659 2_2:-61778152 Msa0262740:CDS
AGAATCATAGTGAAGCAGAG+AGG 0.708784 2_2:-61778097 Msa0262740:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GGAATAGAATAATTTAATTT+TGG - chr2_2:61777761-61777780 Msa0262740:intron 15.0%
!!! GGAATAGAATAATTTAATTT+TGG - chr2_2:61777761-61777780 Msa0262740:intron 15.0%
!! TAAAGATTACTTCTTATAGT+GGG + chr2_2:61776518-61776537 None:intergenic 20.0%
!! TTGAATGATGAAAAGAAAAA+GGG + chr2_2:61776680-61776699 None:intergenic 20.0%
!! ATTGAATGATGAAAAGAAAA+AGG + chr2_2:61776681-61776700 None:intergenic 20.0%
!!! AATTTTCTTTCCTAAAAGAA+GGG - chr2_2:61777740-61777759 Msa0262740:intron 20.0%
!! ACTTCATCTTATGATAATAA+GGG - chr2_2:61778182-61778201 Msa0262740:CDS 20.0%
!! TAAAGATTACTTCTTATAGT+GGG + chr2_2:61776518-61776537 None:intergenic 20.0%
!! TTGAATGATGAAAAGAAAAA+GGG + chr2_2:61776680-61776699 None:intergenic 20.0%
!! ATTGAATGATGAAAAGAAAA+AGG + chr2_2:61776681-61776700 None:intergenic 20.0%
!!! AATTTTCTTTCCTAAAAGAA+GGG - chr2_2:61777740-61777759 Msa0262740:intron 20.0%
!! ACTTCATCTTATGATAATAA+GGG - chr2_2:61778182-61778201 Msa0262740:CDS 20.0%
! GTAAAGATTACTTCTTATAG+TGG + chr2_2:61776519-61776538 None:intergenic 25.0%
!!! TGTTGTTAATTTGTCATGTT+GGG + chr2_2:61776656-61776675 None:intergenic 25.0%
!!! TTGTTGTTAATTTGTCATGT+TGG + chr2_2:61776657-61776676 None:intergenic 25.0%
!!! TTTTATTTGTGTGTTTGGAA+TGG + chr2_2:61776719-61776738 None:intergenic 25.0%
! TTTGAATTACTAGTTAGTAC+TGG - chr2_2:61777160-61777179 Msa0262740:CDS 25.0%
! TGATTAACTCCAAATATTTG+AGG - chr2_2:61777213-61777232 Msa0262740:intron 25.0%
! GATTAACTCCAAATATTTGA+GGG - chr2_2:61777214-61777233 Msa0262740:intron 25.0%
! ATTAACTCCAAATATTTGAG+GGG - chr2_2:61777215-61777234 Msa0262740:intron 25.0%
! ATTCAAATGACCATAAAAGA+GGG + chr2_2:61777462-61777481 None:intergenic 25.0%
!!! ATGTAGCTTTTAGTTTTCAA+TGG - chr2_2:61777708-61777727 Msa0262740:intron 25.0%
! TTAGGAAAGAAAATTGAACT+TGG + chr2_2:61777735-61777754 None:intergenic 25.0%
!! CAATTTTCTTTCCTAAAAGA+AGG - chr2_2:61777739-61777758 Msa0262740:intron 25.0%
!! TTATTCTATTCCCTTCTTTT+AGG + chr2_2:61777753-61777772 None:intergenic 25.0%
! AAGTATAAGCATAACTAAGT+TGG + chr2_2:61777844-61777863 None:intergenic 25.0%
! AACTTAGTTATGCTTATACT+TGG - chr2_2:61777843-61777862 Msa0262740:intron 25.0%
!!! GTGTACTTTTTTAACTTAGT+TGG - chr2_2:61777939-61777958 Msa0262740:intron 25.0%
! ATGATGCTCTAATTGAATAA+GGG + chr2_2:61778107-61778126 None:intergenic 25.0%
! AATGATGCTCTAATTGAATA+AGG + chr2_2:61778108-61778127 None:intergenic 25.0%
! TCTTATATCAGACAATAAAC+CGG + chr2_2:61778148-61778167 None:intergenic 25.0%
! CACTTCATCTTATGATAATA+AGG - chr2_2:61778181-61778200 Msa0262740:CDS 25.0%
! ATATTCAGTTCTTCATCTTT+AGG - chr2_2:61778396-61778415 Msa0262740:five_prime_UTR 25.0%
! AAAGATTACTTCTTATAGTG+GGG + chr2_2:61776517-61776536 None:intergenic 25.0%
! GTAAAGATTACTTCTTATAG+TGG + chr2_2:61776519-61776538 None:intergenic 25.0%
!!! TGTTGTTAATTTGTCATGTT+GGG + chr2_2:61776656-61776675 None:intergenic 25.0%
!!! TTGTTGTTAATTTGTCATGT+TGG + chr2_2:61776657-61776676 None:intergenic 25.0%
!!! TTTTATTTGTGTGTTTGGAA+TGG + chr2_2:61776719-61776738 None:intergenic 25.0%
! TTTGAATTACTAGTTAGTAC+TGG - chr2_2:61777160-61777179 Msa0262740:CDS 25.0%
! TGATTAACTCCAAATATTTG+AGG - chr2_2:61777213-61777232 Msa0262740:intron 25.0%
! GATTAACTCCAAATATTTGA+GGG - chr2_2:61777214-61777233 Msa0262740:intron 25.0%
! ATTAACTCCAAATATTTGAG+GGG - chr2_2:61777215-61777234 Msa0262740:intron 25.0%
! ATTCAAATGACCATAAAAGA+GGG + chr2_2:61777462-61777481 None:intergenic 25.0%
!!! ATGTAGCTTTTAGTTTTCAA+TGG - chr2_2:61777708-61777727 Msa0262740:intron 25.0%
! TTAGGAAAGAAAATTGAACT+TGG + chr2_2:61777735-61777754 None:intergenic 25.0%
!! CAATTTTCTTTCCTAAAAGA+AGG - chr2_2:61777739-61777758 Msa0262740:intron 25.0%
!! TTATTCTATTCCCTTCTTTT+AGG + chr2_2:61777753-61777772 None:intergenic 25.0%
! AAGTATAAGCATAACTAAGT+TGG + chr2_2:61777844-61777863 None:intergenic 25.0%
! AACTTAGTTATGCTTATACT+TGG - chr2_2:61777843-61777862 Msa0262740:intron 25.0%
!!! GTGTACTTTTTTAACTTAGT+TGG - chr2_2:61777939-61777958 Msa0262740:intron 25.0%
! ATGATGCTCTAATTGAATAA+GGG + chr2_2:61778107-61778126 None:intergenic 25.0%
! AATGATGCTCTAATTGAATA+AGG + chr2_2:61778108-61778127 None:intergenic 25.0%
! TCTTATATCAGACAATAAAC+CGG + chr2_2:61778148-61778167 None:intergenic 25.0%
! CACTTCATCTTATGATAATA+AGG - chr2_2:61778181-61778200 Msa0262740:CDS 25.0%
! ATATTCAGTTCTTCATCTTT+AGG - chr2_2:61778396-61778415 Msa0262740:five_prime_UTR 25.0%
! TATGATGATGATAGTGAGTT+TGG + chr2_2:61776597-61776616 None:intergenic 30.0%
!! GTTGTTAATTTGTCATGTTG+GGG + chr2_2:61776655-61776674 None:intergenic 30.0%
!!! GTGTTGTTTTTCAATGGAAA+TGG - chr2_2:61776830-61776849 Msa0262740:CDS 30.0%
!! ATGAATGATGGTTGTTTTCA+AGG - chr2_2:61776873-61776892 Msa0262740:CDS 30.0%
ATTCTTTCCCCTCAAATATT+TGG + chr2_2:61777225-61777244 None:intergenic 30.0%
!!! GTCTAGTTTTTGTACTTCAT+AGG + chr2_2:61777327-61777346 None:intergenic 30.0%
ACACTTTAACAGGGAATATA+AGG - chr2_2:61777432-61777451 Msa0262740:intron 30.0%
CATTCAAATGACCATAAAAG+AGG + chr2_2:61777463-61777482 None:intergenic 30.0%
TATAAGTCTTGAGCTATGAA+TGG - chr2_2:61777542-61777561 Msa0262740:intron 30.0%
! CAAAATGCAGTGAGTATTTT+TGG - chr2_2:61777570-61777589 Msa0262740:intron 30.0%
AGTTAAATTGAGACAGTGAA+TGG - chr2_2:61777609-61777628 Msa0262740:intron 30.0%
! ACAGTGAATGGCAAAATTTT+GGG - chr2_2:61777621-61777640 Msa0262740:intron 30.0%
! AATGGCTTTACTCACATATA+TGG + chr2_2:61777649-61777668 None:intergenic 30.0%
GACTTCAAAAGTAAGCAAAA+TGG + chr2_2:61777667-61777686 None:intergenic 30.0%
TTGTGTAGACATGAATTTCA+TGG + chr2_2:61777871-61777890 None:intergenic 30.0%
ATAAGAGTTGAATCACAAGA+AGG - chr2_2:61778066-61778085 Msa0262740:CDS 30.0%
TTATGTTGTGAATACAAACC+CGG - chr2_2:61778126-61778145 Msa0262740:CDS 30.0%
CTTATATCAGACAATAAACC+GGG + chr2_2:61778147-61778166 None:intergenic 30.0%
TCTTAGAAACTAGAAAGAGA+AGG - chr2_2:61778364-61778383 Msa0262740:five_prime_UTR 30.0%
TTCACATGTAATGTGAATCT+TGG - chr2_2:61778451-61778470 Msa0262740:five_prime_UTR 30.0%
TCACATGTAATGTGAATCTT+GGG - chr2_2:61778452-61778471 Msa0262740:five_prime_UTR 30.0%
ACAATGAATGAACTCATTCT+AGG - chr2_2:61778555-61778574 Msa0262740:five_prime_UTR 30.0%
AAGATTACTTCTTATAGTGG+GGG + chr2_2:61776516-61776535 None:intergenic 30.0%
! TATGATGATGATAGTGAGTT+TGG + chr2_2:61776597-61776616 None:intergenic 30.0%
!! GTTGTTAATTTGTCATGTTG+GGG + chr2_2:61776655-61776674 None:intergenic 30.0%
!!! GTGTTGTTTTTCAATGGAAA+TGG - chr2_2:61776830-61776849 Msa0262740:CDS 30.0%
!! ATGAATGATGGTTGTTTTCA+AGG - chr2_2:61776873-61776892 Msa0262740:CDS 30.0%
ATTCTTTCCCCTCAAATATT+TGG + chr2_2:61777225-61777244 None:intergenic 30.0%
!!! GTCTAGTTTTTGTACTTCAT+AGG + chr2_2:61777327-61777346 None:intergenic 30.0%
ACACTTTAACAGGGAATATA+AGG - chr2_2:61777432-61777451 Msa0262740:intron 30.0%
CATTCAAATGACCATAAAAG+AGG + chr2_2:61777463-61777482 None:intergenic 30.0%
TATAAGTCTTGAGCTATGAA+TGG - chr2_2:61777542-61777561 Msa0262740:intron 30.0%
! CAAAATGCAGTGAGTATTTT+TGG - chr2_2:61777570-61777589 Msa0262740:intron 30.0%
AGTTAAATTGAGACAGTGAA+TGG - chr2_2:61777609-61777628 Msa0262740:intron 30.0%
! ACAGTGAATGGCAAAATTTT+GGG - chr2_2:61777621-61777640 Msa0262740:intron 30.0%
! AATGGCTTTACTCACATATA+TGG + chr2_2:61777649-61777668 None:intergenic 30.0%
GACTTCAAAAGTAAGCAAAA+TGG + chr2_2:61777667-61777686 None:intergenic 30.0%
TTGTGTAGACATGAATTTCA+TGG + chr2_2:61777871-61777890 None:intergenic 30.0%
ATAAGAGTTGAATCACAAGA+AGG - chr2_2:61778066-61778085 Msa0262740:CDS 30.0%
TTATGTTGTGAATACAAACC+CGG - chr2_2:61778126-61778145 Msa0262740:CDS 30.0%
CTTATATCAGACAATAAACC+GGG + chr2_2:61778147-61778166 None:intergenic 30.0%
TCTTAGAAACTAGAAAGAGA+AGG - chr2_2:61778364-61778383 Msa0262740:five_prime_UTR 30.0%
TTCACATGTAATGTGAATCT+TGG - chr2_2:61778451-61778470 Msa0262740:five_prime_UTR 30.0%
TCACATGTAATGTGAATCTT+GGG - chr2_2:61778452-61778471 Msa0262740:five_prime_UTR 30.0%
ACAATGAATGAACTCATTCT+AGG - chr2_2:61778555-61778574 Msa0262740:five_prime_UTR 30.0%
!! TTAATTTGTCATGTTGGGGT+TGG + chr2_2:61776651-61776670 None:intergenic 35.0%
!!! GGCTGTTTTATTTGTGTGTT+TGG + chr2_2:61776724-61776743 None:intergenic 35.0%
!!! GGAACTGTGTTGTTTTTCAA+TGG - chr2_2:61776824-61776843 Msa0262740:CDS 35.0%
GCTTCAAGTAGAATGAATGA+TGG - chr2_2:61776861-61776880 Msa0262740:CDS 35.0%
!! GGTTGTTTTCAAGGTGTAAA+TGG - chr2_2:61776882-61776901 Msa0262740:CDS 35.0%
TTTCAAGGTGTAAATGGAAG+TGG - chr2_2:61776888-61776907 Msa0262740:CDS 35.0%
ATATGGTTCATCTTCTTCAC+TGG - chr2_2:61776911-61776930 Msa0262740:CDS 35.0%
!!! TTCATCTTCTTCACTGGTTT+TGG - chr2_2:61776917-61776936 Msa0262740:CDS 35.0%
!!! CTGGTTTTGGATAGAGAAAA+AGG - chr2_2:61776930-61776949 Msa0262740:CDS 35.0%
AAAGCTTCAATACTTCTCTC+AGG + chr2_2:61777002-61777021 None:intergenic 35.0%
! TGTTATTCCTGGTGCTAATA+AGG - chr2_2:61777091-61777110 Msa0262740:CDS 35.0%
! GACAGTGAATGGCAAAATTT+TGG - chr2_2:61777620-61777639 Msa0262740:intron 35.0%
TGTAATAACTTCAGCCAGTA+AGG + chr2_2:61777974-61777993 None:intergenic 35.0%
! CTTATCTCATCACTGTCTTT+TGG + chr2_2:61778051-61778070 None:intergenic 35.0%
!! TCACAAGAAGGTGGATTAAA+TGG - chr2_2:61778078-61778097 Msa0262740:CDS 35.0%
TAGAAACTAGAAAGAGAAGG+AGG - chr2_2:61778367-61778386 Msa0262740:five_prime_UTR 35.0%
! ATCTTGGGTTGTACATTTGT+TGG - chr2_2:61778467-61778486 Msa0262740:five_prime_UTR 35.0%
! TCTTGGGTTGTACATTTGTT+GGG - chr2_2:61778468-61778487 Msa0262740:five_prime_UTR 35.0%
! GGGAACTTTTGTAGATAGTT+AGG - chr2_2:61778488-61778507 Msa0262740:five_prime_UTR 35.0%
! AGATTACTTCTTATAGTGGG+GGG + chr2_2:61776515-61776534 None:intergenic 35.0%
!! TTAATTTGTCATGTTGGGGT+TGG + chr2_2:61776651-61776670 None:intergenic 35.0%
!!! GGCTGTTTTATTTGTGTGTT+TGG + chr2_2:61776724-61776743 None:intergenic 35.0%
!!! GGAACTGTGTTGTTTTTCAA+TGG - chr2_2:61776824-61776843 Msa0262740:CDS 35.0%
GCTTCAAGTAGAATGAATGA+TGG - chr2_2:61776861-61776880 Msa0262740:CDS 35.0%
!! GGTTGTTTTCAAGGTGTAAA+TGG - chr2_2:61776882-61776901 Msa0262740:CDS 35.0%
TTTCAAGGTGTAAATGGAAG+TGG - chr2_2:61776888-61776907 Msa0262740:CDS 35.0%
ATATGGTTCATCTTCTTCAC+TGG - chr2_2:61776911-61776930 Msa0262740:CDS 35.0%
!!! TTCATCTTCTTCACTGGTTT+TGG - chr2_2:61776917-61776936 Msa0262740:CDS 35.0%
!!! CTGGTTTTGGATAGAGAAAA+AGG - chr2_2:61776930-61776949 Msa0262740:CDS 35.0%
AAAGCTTCAATACTTCTCTC+AGG + chr2_2:61777002-61777021 None:intergenic 35.0%
! TGTTATTCCTGGTGCTAATA+AGG - chr2_2:61777091-61777110 Msa0262740:CDS 35.0%
! GACAGTGAATGGCAAAATTT+TGG - chr2_2:61777620-61777639 Msa0262740:intron 35.0%
TGTAATAACTTCAGCCAGTA+AGG + chr2_2:61777974-61777993 None:intergenic 35.0%
! CTTATCTCATCACTGTCTTT+TGG + chr2_2:61778051-61778070 None:intergenic 35.0%
!! TCACAAGAAGGTGGATTAAA+TGG - chr2_2:61778078-61778097 Msa0262740:CDS 35.0%
TAGAAACTAGAAAGAGAAGG+AGG - chr2_2:61778367-61778386 Msa0262740:five_prime_UTR 35.0%
! ATCTTGGGTTGTACATTTGT+TGG - chr2_2:61778467-61778486 Msa0262740:five_prime_UTR 35.0%
! TCTTGGGTTGTACATTTGTT+GGG - chr2_2:61778468-61778487 Msa0262740:five_prime_UTR 35.0%
! GGGAACTTTTGTAGATAGTT+AGG - chr2_2:61778488-61778507 Msa0262740:five_prime_UTR 35.0%
!! TTTGCTGTTGATGCTTCTTC+TGG + chr2_2:61776541-61776560 None:intergenic 40.0%
AAGACAACGTTGGAAGAAGA+AGG + chr2_2:61776760-61776779 None:intergenic 40.0%
GACACAAAGAAAGACAACGT+TGG + chr2_2:61776770-61776789 None:intergenic 40.0%
CATATTCTGAACACCCTCTT+GGG - chr2_2:61776803-61776822 Msa0262740:CDS 40.0%
AAACAACACAGTTCCCAAGA+GGG + chr2_2:61776819-61776838 None:intergenic 40.0%
AAAACAACACAGTTCCCAAG+AGG + chr2_2:61776820-61776839 None:intergenic 40.0%
GGTGTAAATGGAAGTGGATA+TGG - chr2_2:61776894-61776913 Msa0262740:CDS 40.0%
TAGAGAAAAAGGAGAGCTTG+TGG - chr2_2:61776941-61776960 Msa0262740:CDS 40.0%
AGAATCATAGTGAAGCAGAG+AGG - chr2_2:61777024-61777043 Msa0262740:CDS 40.0%
! ACACTTCGTAGTGTTATTCC+TGG - chr2_2:61777080-61777099 Msa0262740:CDS 40.0%
GAACTAACCTTATTAGCACC+AGG + chr2_2:61777101-61777120 None:intergenic 40.0%
AAGACTCGCACACTTTAACA+GGG - chr2_2:61777423-61777442 Msa0262740:intron 40.0%
! ATAAGGACGACCCTCTTTTA+TGG - chr2_2:61777449-61777468 Msa0262740:intron 40.0%
CAAGAGCTCAATGAGTTTGA+TGG - chr2_2:61777789-61777808 Msa0262740:intron 40.0%
AGAGTTGAATCACAAGAAGG+TGG - chr2_2:61778069-61778088 Msa0262740:CDS 40.0%
AGCAAGAAACTGTCGATACT+CGG + chr2_2:61778286-61778305 None:intergenic 40.0%
!! TTTGCTGTTGATGCTTCTTC+TGG + chr2_2:61776541-61776560 None:intergenic 40.0%
AAGACAACGTTGGAAGAAGA+AGG + chr2_2:61776760-61776779 None:intergenic 40.0%
GACACAAAGAAAGACAACGT+TGG + chr2_2:61776770-61776789 None:intergenic 40.0%
CATATTCTGAACACCCTCTT+GGG - chr2_2:61776803-61776822 Msa0262740:CDS 40.0%
AAACAACACAGTTCCCAAGA+GGG + chr2_2:61776819-61776838 None:intergenic 40.0%
AAAACAACACAGTTCCCAAG+AGG + chr2_2:61776820-61776839 None:intergenic 40.0%
GGTGTAAATGGAAGTGGATA+TGG - chr2_2:61776894-61776913 Msa0262740:CDS 40.0%
TAGAGAAAAAGGAGAGCTTG+TGG - chr2_2:61776941-61776960 Msa0262740:CDS 40.0%
AGAATCATAGTGAAGCAGAG+AGG - chr2_2:61777024-61777043 Msa0262740:CDS 40.0%
! ACACTTCGTAGTGTTATTCC+TGG - chr2_2:61777080-61777099 Msa0262740:CDS 40.0%
GAACTAACCTTATTAGCACC+AGG + chr2_2:61777101-61777120 None:intergenic 40.0%
AAGACTCGCACACTTTAACA+GGG - chr2_2:61777423-61777442 Msa0262740:intron 40.0%
! ATAAGGACGACCCTCTTTTA+TGG - chr2_2:61777449-61777468 Msa0262740:intron 40.0%
CAAGAGCTCAATGAGTTTGA+TGG - chr2_2:61777789-61777808 Msa0262740:intron 40.0%
AGAGTTGAATCACAAGAAGG+TGG - chr2_2:61778069-61778088 Msa0262740:CDS 40.0%
AGCAAGAAACTGTCGATACT+CGG + chr2_2:61778286-61778305 None:intergenic 40.0%
TGTTGGGGTTGGAGTGTATT+TGG + chr2_2:61776640-61776659 None:intergenic 45.0%
GAAGAAGGATGAGGAGACAA+AGG + chr2_2:61776745-61776764 None:intergenic 45.0%
ACGTTGGAAGAAGAAGGATG+AGG + chr2_2:61776754-61776773 None:intergenic 45.0%
!! CCAAGAGGGTGTTCAGAATA+TGG + chr2_2:61776805-61776824 None:intergenic 45.0%
CCATATTCTGAACACCCTCT+TGG - chr2_2:61776802-61776821 Msa0262740:CDS 45.0%
AGAAAAAGGAGAGCTTGTGG+AGG - chr2_2:61776944-61776963 Msa0262740:CDS 45.0%
GCTGTGAAATTGGAACGCAA+AGG - chr2_2:61776969-61776988 Msa0262740:CDS 45.0%
ATCATAGTGAAGCAGAGAGG+AGG - chr2_2:61777027-61777046 Msa0262740:CDS 45.0%
TAAAGTGTGCGAGTCTTCAC+AGG + chr2_2:61777420-61777439 None:intergenic 45.0%
GAAGACTCGCACACTTTAAC+AGG - chr2_2:61777422-61777441 Msa0262740:intron 45.0%
! GTTGGACAAAGCATCCTTAC+TGG - chr2_2:61777957-61777976 Msa0262740:intron 45.0%
AATGCAGCACAAGCAAGTGA+AGG - chr2_2:61778015-61778034 Msa0262740:intron 45.0%
AAGGGCAGAGATTGCAACTT+TGG - chr2_2:61778200-61778219 Msa0262740:CDS 45.0%
AGGGCAGAGATTGCAACTTT+GGG - chr2_2:61778201-61778220 Msa0262740:CDS 45.0%
!! GAGTATCGACAGTTTCTTGC+TGG - chr2_2:61778285-61778304 Msa0262740:exon 45.0%
TCGACAGTTTCTTGCTGGAT+CGG - chr2_2:61778290-61778309 Msa0262740:exon 45.0%
TGTTGGGGTTGGAGTGTATT+TGG + chr2_2:61776640-61776659 None:intergenic 45.0%
GAAGAAGGATGAGGAGACAA+AGG + chr2_2:61776745-61776764 None:intergenic 45.0%
ACGTTGGAAGAAGAAGGATG+AGG + chr2_2:61776754-61776773 None:intergenic 45.0%
!! CCAAGAGGGTGTTCAGAATA+TGG + chr2_2:61776805-61776824 None:intergenic 45.0%
CCATATTCTGAACACCCTCT+TGG - chr2_2:61776802-61776821 Msa0262740:CDS 45.0%
AGAAAAAGGAGAGCTTGTGG+AGG - chr2_2:61776944-61776963 Msa0262740:CDS 45.0%
GCTGTGAAATTGGAACGCAA+AGG - chr2_2:61776969-61776988 Msa0262740:CDS 45.0%
ATCATAGTGAAGCAGAGAGG+AGG - chr2_2:61777027-61777046 Msa0262740:CDS 45.0%
TAAAGTGTGCGAGTCTTCAC+AGG + chr2_2:61777420-61777439 None:intergenic 45.0%
GAAGACTCGCACACTTTAAC+AGG - chr2_2:61777422-61777441 Msa0262740:intron 45.0%
! GTTGGACAAAGCATCCTTAC+TGG - chr2_2:61777957-61777976 Msa0262740:intron 45.0%
AATGCAGCACAAGCAAGTGA+AGG - chr2_2:61778015-61778034 Msa0262740:intron 45.0%
AAGGGCAGAGATTGCAACTT+TGG - chr2_2:61778200-61778219 Msa0262740:CDS 45.0%
AGGGCAGAGATTGCAACTTT+GGG - chr2_2:61778201-61778220 Msa0262740:CDS 45.0%
!! GAGTATCGACAGTTTCTTGC+TGG - chr2_2:61778285-61778304 Msa0262740:exon 45.0%
TCGACAGTTTCTTGCTGGAT+CGG - chr2_2:61778290-61778309 Msa0262740:exon 45.0%
! GTGAGTTTGGTGAGATCCAG+AGG + chr2_2:61776584-61776603 None:intergenic 50.0%
TGTGGAGGCAGCTGTGAAAT+TGG - chr2_2:61776959-61776978 Msa0262740:CDS 50.0%
GCAGAGATTGCAACTTTGGG+AGG - chr2_2:61778204-61778223 Msa0262740:CDS 50.0%
! GTGAGTTTGGTGAGATCCAG+AGG + chr2_2:61776584-61776603 None:intergenic 50.0%
TGTGGAGGCAGCTGTGAAAT+TGG - chr2_2:61776959-61776978 Msa0262740:CDS 50.0%
GCAGAGATTGCAACTTTGGG+AGG - chr2_2:61778204-61778223 Msa0262740:CDS 50.0%
AGCACTACTCTCTCTGCCTC+TGG - chr2_2:61776565-61776584 Msa0262740:three_prime_UTR 55.0%
AGCACTACTCTCTCTGCCTC+TGG - chr2_2:61776565-61776584 Msa0262740:three_prime_UTR 55.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 61776515 61778628 61776515 ID=Msa0262740;Name=Msa0262740
chr2_2 mRNA 61776515 61778628 61776515 ID=Msa0262740-mRNA-1;Parent=Msa0262740;Name=Msa0262740-mRNA-1;_AED=0.01;_eAED=0.01;_QI=328|1|1|1|1|1|2|197|247
chr2_2 exon 61776515 61777185 61776515 ID=Msa0262740-mRNA-1:exon:19005;Parent=Msa0262740-mRNA-1
chr2_2 exon 61778031 61778628 61778031 ID=Msa0262740-mRNA-1:exon:19004;Parent=Msa0262740-mRNA-1
chr2_2 five_prime_UTR 61778301 61778628 61778301 ID=Msa0262740-mRNA-1:five_prime_utr;Parent=Msa0262740-mRNA-1
chr2_2 CDS 61778031 61778300 61778031 ID=Msa0262740-mRNA-1:cds;Parent=Msa0262740-mRNA-1
chr2_2 CDS 61776712 61777185 61776712 ID=Msa0262740-mRNA-1:cds;Parent=Msa0262740-mRNA-1
chr2_2 three_prime_UTR 61776515 61776711 61776515 ID=Msa0262740-mRNA-1:three_prime_utr;Parent=Msa0262740-mRNA-1
Gene Sequence

>Msa0262740

ATGGAAATGGATGATTTTGCTTCAAGTAGAATGAATGATGGTTGTTTTCAAGGTGTAAATGGAAGTGGATATGGTTCATCTTCTTCACTGGTTTTGGATAGAGAAAAAGGAGAGCTTGTGGAGGCAGCTGTGAAATTGGAACGCAAAGGTGTGTCGCCTGAGAGAAGTATTGAAGCTTTGAAGAATCATAGTGAAGCAGAGAGGAGGAGAAGAGCAAGAATTAATTCACATCTTGATACACTTCGTAGTGTTATTCCTGGTGCTAATAAGTTGGACAAAGCATCCTTACTGGCTGAAGTTATTACACATTTGAAAGAGCTGAAAACAAATGCAGCACAAGCAAGTGAAGGATTAATTATTCCAAAAGACAGTGATGAGATAAGAGTTGAATCACAAGAAGGTGGATTAAATGGTTACCCTTATTCAATTAGAGCATCATTATGTTGTGAATACAAACCCGGTTTATTGTCTGATATAAGAAAAGCACTTGATGCACTTCATCTTATGATAATAAGGGCAGAGATTGCAACTTTGGGAGGTAGAATGAAGAATGTCTTTGTCATAATTAGCTGCAAAGAACAGAATTTTGAAGATGCCGAGTATCGACAGTTTCTTGCTGGATCGGTTCATCAAGCTCTTAGATCTGTACTTGATAGATTTTCTGTTTCACAAGATATCTTAGAAACTAGAAAGAGAAGGAGGATTTCTATATTCAGTTCTTCATCTTTAGGTGATTTCTTATAA
Protein sequence

>Msa0262740

MEMDDFASSRMNDGCFQGVNGSGYGSSSSLVLDREKGELVEAAVKLERKGVSPERSIEALKNHSEAERRRRARINSHLDTLRSVIPGANKLDKASLLAEVITHLKELKTNAAQASEGLIIPKDSDEIRVESQEGGLNGYPYSIRASLCCEYKPGLLSDIRKALDALHLMIIRAEIATLGGRMKNVFVIISCKEQNFEDAEYRQFLAGSVHQALRSVLDRFSVSQDILETRKRRRISIFSSSSLGDFL*