AlfalfaGEDB | Msa0264130

M. sativa cultivar zhongmu-4 / Msa0264130

KEGG: K13435
GO: GO:0000902 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009506 GO:0009653 GO:0009719 GO:0009725 GO:0009741 GO:0009826 GO:0009987 GO:0010033 GO:0014070 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0030054 GO:0032502 GO:0032989 GO:0033993 GO:0036211 GO:0040007 GO:0042221 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048589 GO:0048856 GO:0048869 GO:0050896 GO:0055044 GO:0060560 GO:0071704 GO:0071840 GO:0071944 GO:0140096 GO:1901564 GO:1901700
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0264130	XP_003597308.1	48.750	160	33	6	3	113	437	596	4.13e-28	118

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0264130	sp\|Q9LX66\|HERK_ARATH	40.506	158	46	7	3	113	422	578	2.55e-19	85.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0264130	G7IUA3	48.750	160	33	6	3	113	437	596	1.97e-28	118

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0264130	MtrunA17_Chr2g0327671	48.750	160	33	6	3	113	437	596	3.80e-32	118
Msa0264130	MtrunA17_Chr4g0030441	73.585	53	13	1	62	113	541	593	8.14e-19	80.5
Msa0264130	MtrunA17_Chr2g0318301	51.250	80	37	2	35	113	517	595	4.74e-16	72.8
Msa0264130	MtrunA17_Chr8g0352381	71.429	42	12	0	62	103	572	613	7.71e-15	69.3
Msa0264130	MtrunA17_Chr2g0292501	50.725	69	33	1	35	103	536	603	9.63e-15	68.9
Msa0264130	MtrunA17_Chr7g0245131	46.591	88	30	3	35	113	550	629	3.90e-14	67.4
Msa0264130	MtrunA17_Chr4g0061081	64.286	42	15	0	62	103	568	609	8.23e-14	66.2
Msa0264130	MtrunA17_Chr4g0053561	60.377	53	20	1	62	113	482	534	5.46e-13	63.9
Msa0264130	MtrunA17_Chr4g0053521	65.909	44	15	0	60	103	1098	1141	6.43e-13	63.9
Msa0264130	MtrunA17_Chr7g0274261	61.905	42	16	0	62	103	568	609	2.95e-12	62.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0264130	AT3G46290.1	40.506	158	46	7	3	113	422	578	2.60e-20	85.5
Msa0264130	AT5G59700.1	85.714	42	6	0	62	103	523	564	8.72e-19	80.9
Msa0264130	AT5G54380.1	66.038	53	17	1	62	113	551	603	8.19e-16	72.4
Msa0264130	AT2G39360.1	71.429	42	12	0	62	103	528	569	9.08e-16	72.4
Msa0264130	AT5G61350.1	53.623	69	31	1	35	103	540	607	1.52e-15	71.6
Msa0264130	AT1G30570.1	73.810	42	11	0	62	103	561	602	5.87e-15	70.1
Msa0264130	AT3G51550.1	46.591	88	30	3	35	113	551	630	1.19e-14	69.3
Msa0264130	AT2G21480.1	71.429	42	12	0	62	103	566	607	2.73e-14	68.2
Msa0264130	AT4G39110.1	69.048	42	13	0	62	103	567	608	3.01e-14	68.2
Msa0264130	AT5G39000.1	50.000	72	30	2	35	103	533	601	5.54e-14	67.4

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Find 35 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACTCTCAGAGTCGTCTACA+CGG	0.596763	2_2:+63985324	Msa0264130:CDS
AATGATACAATCACATACAA+TGG	0.537213	2_2:+63984980	Msa0264130:CDS
ACTCCCAAGCTTAAGTTGGA+AGG	0.605304	2_2:+63985352	Msa0264130:CDS
AGGAAATTGGTAGTGAAGAG+AGG	0.440075	2_2:+63985164	Msa0264130:CDS
AGGGAATACACAGTTGCACC+AGG	0.539966	2_2:+63985184	Msa0264130:CDS
AGGGAGAGTTAATGGATGGC+AGG	0.538987	2_2:+63985144	Msa0264130:CDS
AGTATACAAGGGAGAGTTAA+TGG	0.311117	2_2:+63985136	Msa0264130:intron
ATACATGAATACATGGTGAA+CGG	0.627490	2_2:+63985301	Msa0264130:CDS
ATGATACAATCACATACAAT+GGG	0.503298	2_2:+63984981	Msa0264130:CDS
ATTGTATCATTGACGGATAA+TGG	0.409966	2_2:-63984968	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTATGTGATTGTATCATTGA+CGG	-	chr2_2:63984978-63984997	None:intergenic	30.0%
ATGGGAAGTAAATATTCCAA+TGG	+	chr2_2:63984999-63985018	Msa0264130:intron	30.0%
TTTGTTGAGAGTTGGATTAT+TGG	+	chr2_2:63985095-63985114	Msa0264130:intron	30.0%
TTGTTGAGAGTTGGATTATT+GGG	+	chr2_2:63985096-63985115	Msa0264130:intron	30.0%
GATCCTTATACATGAATACA+TGG	+	chr2_2:63985294-63985313	Msa0264130:CDS	30.0%
TCACCATGTATTCATGTATA+AGG	-	chr2_2:63985300-63985319	None:intergenic	30.0%
ATACATGAATACATGGTGAA+CGG	+	chr2_2:63985301-63985320	Msa0264130:CDS	30.0%
CCATCTTGAACTTATGCAAA+AGG	-	chr2_2:63985065-63985084	None:intergenic	35.0%
TGTTGAGAGTTGGATTATTG+GGG	+	chr2_2:63985097-63985116	Msa0264130:intron	35.0%
AGTATACAAGGGAGAGTTAA+TGG	+	chr2_2:63985136-63985155	Msa0264130:intron	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_2	gene	63984893	63985384	63984893	ID=Msa0264130;Name=Msa0264130
chr2_2	mRNA	63984893	63985384	63984893	ID=Msa0264130-mRNA-1;Parent=Msa0264130;Name=Msa0264130-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0\|0\|0\|1\|0\|0.5\|2\|0\|119
chr2_2	exon	63984893	63985005	63984893	ID=Msa0264130-mRNA-1:exon:19895;Parent=Msa0264130-mRNA-1
chr2_2	exon	63985138	63985384	63985138	ID=Msa0264130-mRNA-1:exon:19896;Parent=Msa0264130-mRNA-1
chr2_2	CDS	63984893	63985005	63984893	ID=Msa0264130-mRNA-1:cds;Parent=Msa0264130-mRNA-1
chr2_2	CDS	63985138	63985384	63985138	ID=Msa0264130-mRNA-1:cds;Parent=Msa0264130-mRNA-1

Gene Sequence	>Msa0264130 ATGCCCGGAGTTTGTTGTGTACTGTGCAGGAAAAGGAAGTGGCTGGCAAGACAAAGGCAGTCAAAGACACGGATTCCATTATCCGTCAATGATACAATCACATACAATGGGAATATACAAGGGAGAGTTAATGGATGGCAGGAAATTGGTAGTGAAGAGAGGGAATACACAGTTGCACCAGGGTTTCGAACTGAAATTGAAATGTTATCTCAGTTCTGCCTGCGACATCTGGTGTCTTTGATTGGTTATTGTGATGAAAATAATGAAATGATCCTTATACATGAATACATGGTGAACGGAACTCTCAGAGTCGTCTACACGGTCGGGACTCCCAAGCTTAAGTTGGAAGGAGAGACTTGA
Protein sequence	>Msa0264130 MPGVCCVLCRKRKWLARQRQSKTRIPLSVNDTITYNGNIQGRVNGWQEIGSEEREYTVAPGFRTEIEMLSQFCLRHLVSLIGYCDENNEMILIHEYMVNGTLRVVYTVGTPKLKLEGET*