AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0264470

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0264470 MtrunA17_Chr2g0328381 94.582 646 32 1 274 919 1 643 0.0 1240
Msa0264470 MtrunA17_Chr2g0328381 72.954 281 57 4 1 274 1 269 6.05e-114 363
Msa0264470 MtrunA17_Chr2g0328341 83.667 649 94 4 274 919 1 640 0.0 1095
Msa0264470 MtrunA17_Chr2g0328341 84.477 277 29 2 1 274 1 266 3.59e-136 421
Msa0264470 MtrunA17_Chr2g0328361 78.891 649 102 4 274 919 1 617 0.0 988
Msa0264470 MtrunA17_Chr2g0328361 70.609 279 66 5 1 274 1 268 1.50e-103 335
Msa0264470 MtrunA17_Chr2g0328371 86.905 252 33 0 621 872 1 252 3.41e-160 469
Msa0264470 MtrunA17_Chr2g0328391 44.013 618 312 14 324 919 66 671 1.28e-155 473
Msa0264470 MtrunA17_Chr4g0031701 42.468 624 303 16 324 919 33 628 1.26e-152 464
Msa0264470 MtrunA17_Chr4g0031731 41.627 627 304 15 324 919 33 628 4.80e-148 452
Msa0264470 MtrunA17_Chr7g0238891 52.320 388 179 3 536 919 357 742 1.05e-146 452
Msa0264470 MtrunA17_Chr4g0031711 48.519 439 210 7 495 919 299 735 1.55e-143 444
Msa0264470 MtrunA17_Chr4g0031741 46.285 471 226 7 469 919 189 652 1.66e-137 425
Msa0264470 MtrunA17_Chr2g0328431 39.165 623 336 15 323 919 51 656 2.45e-136 422
Msa0264470 MtrunA17_Chr2g0328411 38.915 627 328 16 323 917 51 654 4.32e-135 420
Msa0264470 MtrunA17_Chr2g0328401 49.438 356 171 4 545 896 284 634 1.30e-121 383
Msa0264470 MtrunA17_Chr5g0447691 30.933 375 250 4 547 916 173 543 4.50e-61 217
Msa0264470 MtrunA17_Chr3g0101141 28.839 534 331 16 406 916 34 541 3.06e-58 209
Msa0264470 MtrunA17_Chr4g0076091 30.079 379 253 5 546 917 170 543 2.14e-57 207
Msa0264470 MtrunA17_Chr2g0319091 31.565 377 245 4 547 916 208 578 5.42e-57 206
Msa0264470 MtrunA17_Chr2g0290241 29.765 383 258 5 542 916 45 424 6.67e-57 202
Msa0264470 MtrunA17_Chr3g0125811 29.570 372 255 3 549 916 201 569 2.29e-53 196
Msa0264470 MtrunA17_Chr5g0445331 30.184 381 249 8 547 916 157 531 1.09e-52 193
Msa0264470 MtrunA17_Chr7g0224271 31.218 394 232 8 546 916 295 672 1.37e-52 196
Msa0264470 MtrunA17_Chr1g0159461 29.839 372 254 3 549 916 223 591 1.47e-52 194
Msa0264470 MtrunA17_Chr8g0376381 29.551 379 244 7 543 916 161 521 8.53e-46 173
Msa0264470 MtrunA17_Chr2g0306641 30.377 451 282 12 482 916 52 486 9.01e-44 166
Msa0264470 MtrunA17_Chr4g0057291 26.684 386 259 9 547 918 288 663 9.97e-44 169
Msa0264470 MtrunA17_Chr4g0057891 27.749 382 255 8 548 916 146 519 1.04e-43 166
Msa0264470 MtrunA17_Chr7g0245601 30.959 365 238 8 555 918 442 793 1.32e-43 170
Msa0264470 MtrunA17_Chr8g0357601 27.436 390 255 8 546 919 177 554 6.62e-43 165
Msa0264470 MtrunA17_Chr3g0110971 30.612 392 242 8 541 916 209 586 8.69e-43 165
Msa0264470 MtrunA17_Chr5g0396491 25.225 444 266 9 534 916 39 477 1.57e-38 150
Msa0264470 MtrunA17_Chr4g0038851 24.943 437 263 9 540 916 37 468 1.16e-36 144
Msa0264470 MtrunA17_Chr3g0085681 24.337 415 259 7 552 916 26 435 1.78e-36 143
Msa0264470 MtrunA17_Chr4g0038761 28.075 374 243 10 555 919 88 444 2.55e-32 131
Msa0264470 MtrunA17_Chr4g0039661 27.632 380 248 10 548 916 191 554 1.90e-31 130
Msa0264470 MtrunA17_Chr2g0294461 24.485 388 274 6 543 916 202 584 1.23e-30 128
Msa0264470 MtrunA17_Chr1g0200401 27.411 394 243 13 551 916 80 458 1.86e-30 125
Msa0264470 MtrunA17_Chr5g0401131 24.869 382 269 10 551 918 114 491 3.56e-30 125
Msa0264470 MtrunA17_Chr7g0270031 25.526 380 264 9 546 916 184 553 2.58e-29 124
Msa0264470 MtrunA17_Chr1g0183641 27.704 379 252 11 543 916 115 476 2.65e-29 122
Msa0264470 MtrunA17_Chr7g0236851 26.712 292 195 6 636 917 375 657 1.95e-28 122
Msa0264470 MtrunA17_Chr2g0294471 23.834 386 276 7 543 916 196 575 5.57e-28 120
Msa0264470 MtrunA17_Chr6g0468561 25.397 378 257 10 552 916 296 661 7.68e-26 114
Msa0264470 MtrunA17_Chr5g0424131 26.598 391 249 16 551 916 121 498 2.87e-23 104
Msa0264470 MtrunA17_Chr1g0194621 24.429 438 286 16 504 916 100 517 1.03e-22 103
Msa0264470 MtrunA17_Chr2g0329591 28.094 299 200 6 551 842 74 364 1.68e-21 99.0
Msa0264470 MtrunA17_Chr3g0085361 22.753 356 218 8 611 916 1 349 1.88e-21 97.1
Msa0264470 MtrunA17_Chr4g0053901 22.551 439 279 10 533 919 41 470 5.06e-21 97.4
Msa0264470 MtrunA17_Chr3g0114841 24.814 403 253 14 546 916 115 499 5.09e-21 97.8
Msa0264470 MtrunA17_Chr5g0401421 21.362 426 272 9 548 919 32 448 2.01e-19 92.4
Msa0264470 MtrunA17_Chr8g0383981 23.810 399 269 14 534 919 122 498 7.34e-18 87.8
Msa0264470 MtrunA17_Chr7g0230771 24.868 378 248 11 552 916 376 730 7.45e-18 89.0
Msa0264470 MtrunA17_Chr7g0230731 24.603 378 249 11 552 916 373 727 5.51e-17 85.9
Msa0264470 MtrunA17_Chr4g0012071 24.528 371 249 12 556 916 201 550 7.12e-17 85.1
Msa0264470 MtrunA17_Chr4g0031721 31.013 316 155 12 21 280 4 312 2.28e-13 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0264470 AT2G29060.2 42.055 623 319 11 319 918 53 656 9.26e-164 494
Msa0264470 AT2G29060.1 40.958 647 329 13 319 918 53 693 6.58e-161 488
Msa0264470 AT1G07530.1 51.410 461 204 7 476 919 310 767 8.18e-160 487
Msa0264470 AT1G07520.2 48.098 447 216 8 484 919 90 531 2.86e-139 426
Msa0264470 AT1G07520.4 48.098 447 216 8 484 919 90 531 2.86e-139 426
Msa0264470 AT2G29065.1 41.531 614 325 16 323 919 34 630 1.10e-138 428
Msa0264470 AT1G07520.1 48.098 447 216 8 484 919 254 695 5.63e-138 428
Msa0264470 AT1G07520.3 48.098 447 216 8 484 919 255 696 6.18e-138 428
Msa0264470 AT2G37650.2 48.177 384 191 4 539 918 323 702 1.80e-123 390
Msa0264470 AT2G37650.1 48.177 384 191 4 539 918 335 714 2.04e-123 391
Msa0264470 AT3G46600.3 43.155 431 234 5 493 919 126 549 2.76e-116 367
Msa0264470 AT3G46600.1 43.560 427 230 5 497 919 162 581 3.46e-116 367
Msa0264470 AT5G59450.1 40.481 457 261 6 473 919 145 600 2.49e-113 361
Msa0264470 AT3G46600.2 45.745 376 197 4 548 919 1 373 3.61e-111 347
Msa0264470 AT5G48150.1 33.333 375 242 5 547 916 118 489 4.51e-63 221
Msa0264470 AT5G48150.4 33.333 375 242 5 547 916 118 489 4.51e-63 221
Msa0264470 AT5G48150.2 33.333 375 242 5 547 916 118 489 4.51e-63 221
Msa0264470 AT5G48150.3 33.333 375 242 5 547 916 118 489 4.51e-63 221
Msa0264470 AT2G04890.1 31.818 374 241 4 547 916 49 412 1.64e-62 218
Msa0264470 AT1G50600.3 31.095 402 257 6 524 916 135 525 7.91e-59 211
Msa0264470 AT1G50600.1 31.095 402 257 6 524 916 206 596 1.70e-58 211
Msa0264470 AT1G50600.2 31.095 402 257 6 524 916 210 600 2.50e-58 211
Msa0264470 AT1G21450.1 27.788 529 351 13 401 916 82 592 7.70e-54 198
Msa0264470 AT4G17230.1 29.178 377 260 3 546 918 153 526 2.23e-48 181
Msa0264470 AT4G17230.2 29.178 377 260 3 546 918 153 526 2.23e-48 181
Msa0264470 AT3G54220.1 31.566 396 254 9 527 918 269 651 1.72e-45 174
Msa0264470 AT2G01570.1 30.311 386 240 11 541 916 214 580 6.44e-43 166
Msa0264470 AT1G66350.1 30.287 383 238 10 541 916 145 505 3.03e-42 162
Msa0264470 AT3G03450.1 27.720 386 255 8 541 916 173 544 1.88e-41 160
Msa0264470 AT1G14920.1 28.534 382 252 8 541 916 162 528 2.95e-41 160
Msa0264470 AT5G17490.1 26.190 378 261 7 543 916 152 515 7.62e-39 152
Msa0264470 AT1G50420.1 23.131 428 261 9 551 916 57 478 9.88e-35 139
Msa0264470 AT1G63100.1 29.006 362 232 11 539 891 244 589 1.59e-34 141
Msa0264470 AT1G63100.2 29.006 362 232 11 539 891 269 614 1.61e-34 141
Msa0264470 AT5G66770.1 29.600 375 236 12 555 916 224 583 7.31e-33 135
Msa0264470 AT5G41920.1 29.610 385 234 14 546 916 38 399 3.62e-31 127
Msa0264470 AT4G37650.1 25.278 360 235 9 550 884 145 495 2.06e-30 127
Msa0264470 AT3G50650.1 25.895 363 236 10 571 916 195 541 1.79e-27 118
Msa0264470 AT5G52510.1 25.193 389 255 12 549 916 266 639 3.35e-27 118
Msa0264470 AT3G49950.1 24.570 407 281 9 531 918 10 409 1.05e-23 105
Msa0264470 AT4G08250.1 25.762 361 242 12 570 916 128 476 1.25e-23 106
Msa0264470 AT1G55580.1 25.604 414 253 11 548 916 41 444 2.62e-23 104
Msa0264470 AT3G60630.1 28.571 210 125 8 622 817 327 525 4.55e-11 67.0

Find 156 sgRNAs with CRISPR-Local

Find 289 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGAAAATGGAGGAGTATT+TGG 0.132396 2_2:-64418157 None:intergenic
TCTTAATCTGGTTTAATATT+TGG 0.136631 2_2:-64418546 None:intergenic
TTCTATTTGGTTCTTCTTGC+TGG 0.149493 2_2:+64419568 Msa0264470:CDS
TTCACTCTCTCCAATGAATT+TGG 0.151096 2_2:-64418922 None:intergenic
AGTCTTCACATTATAGATTT+TGG 0.160984 2_2:+64418805 Msa0264470:CDS
ATGCTATGAATAAATATATT+TGG 0.170780 2_2:-64419171 None:intergenic
AATCTTCTTCCATGAGAAAT+TGG 0.197500 2_2:-64415470 None:intergenic
AATCTTCTTCCATGAGAAAT+TGG 0.197500 2_2:-64417835 None:intergenic
CAAAGATTGGCACATTTCTT+TGG 0.204755 2_2:+64418598 Msa0264470:CDS
GAATTCTCTTCAACTGTTCT+TGG 0.216798 2_2:+64415342 Msa0264470:CDS
TTTCTCAATGTGTCACAAAA+TGG 0.237333 2_2:+64418280 Msa0264470:CDS
GTGAAGACTTTCTGTTTCTT+TGG 0.238565 2_2:-64418791 None:intergenic
CAAACAGATTCTATGGTTCT+AGG 0.245218 2_2:+64418431 Msa0264470:CDS
TTTATTCATAGCATTGTTAA+TGG 0.248656 2_2:+64419180 Msa0264470:CDS
ACAAAGTTATTAGGAGAAAA+TGG 0.257230 2_2:-64418169 None:intergenic
GAATTCACTTCCACTGTTCT+TGG 0.261009 2_2:+64417707 Msa0264470:CDS
GATTGGGAAACAAAGTTATT+AGG 0.262305 2_2:-64418178 None:intergenic
CATATATGCATTGGCATTCT+AGG 0.265664 2_2:+64419400 Msa0264470:CDS
TTTGATAAAGTACTAGTTCT+TGG 0.268048 2_2:+64418349 Msa0264470:CDS
TGAAATATCATTACAAGAAA+TGG 0.276552 2_2:-64418515 None:intergenic
ATTATTGAAAGAAGGGAAAA+AGG 0.276813 2_2:-64418209 None:intergenic
TTATTTGATAAATTACTTCT+TGG 0.283709 2_2:+64415990 Msa0264470:CDS
ATTCACTCTTACTTTGTTCA+TGG 0.287286 2_2:-64417688 None:intergenic
AAACTATAATCACTGAAGTT+AGG 0.291953 2_2:-64418055 None:intergenic
TAGTTGTATGAGTCTATAAT+TGG 0.294656 2_2:-64415768 None:intergenic
GGAGGGGATGGAAGAGTAGT+TGG 0.296805 2_2:-64415813 None:intergenic
ATTCAGTCTTACTTTGTTCA+TGG 0.302128 2_2:-64415323 None:intergenic
GAATCCGAGAGGTGCTGTTT+TGG 0.303272 2_2:+64419128 Msa0264470:CDS
GAGAAAATGGAGGAGTATTT+GGG 0.303863 2_2:-64418156 None:intergenic
TGTTTGAGGTGAAGGAAAAT+TGG 0.305166 2_2:+64419520 Msa0264470:CDS
TGGAAGGGTAGAATTCTATT+TGG 0.313548 2_2:+64419555 Msa0264470:CDS
GAGAAGAATGTGTCTTAATC+TGG 0.320149 2_2:-64418558 None:intergenic
CATTCTAGGCACATGGGAAA+TGG 0.323083 2_2:+64419414 Msa0264470:CDS
GAATTATCAAACAGATTCTA+TGG 0.344805 2_2:+64418424 Msa0264470:CDS
GATAGATCTTCCAAATTCAT+TGG 0.350686 2_2:+64418912 Msa0264470:CDS
AGAGTAGTTGGGAGTAGAAA+AGG 0.358921 2_2:-64415801 None:intergenic
TTGGGAAAGTATCAAAGTTC+AGG 0.363131 2_2:+64419020 Msa0264470:CDS
TGGGTTTAGATCTTTGAAAC+TGG 0.374406 2_2:+64419434 Msa0264470:CDS
TCTATTTGGTTCTTCTTGCT+GGG 0.374895 2_2:+64419569 Msa0264470:CDS
TCCTCAAACATCAACCTAAC+AGG 0.379387 2_2:-64419306 None:intergenic
GGAGTATTTGGGAGTAGAAA+AGG 0.380526 2_2:-64418145 None:intergenic
CATTATTGAAAGAAGGGAAA+AGG 0.380669 2_2:-64415866 None:intergenic
TTGATAATTCTTCATTCTGC+TGG 0.383176 2_2:-64418411 None:intergenic
ACCTGATGAGACAGTTGTTT+CGG 0.393796 2_2:+64419104 Msa0264470:CDS
TAGCCTGTGAAGGCTGTGAT+AGG 0.399278 2_2:+64419361 Msa0264470:CDS
GCAATACCATTGTACTCAAA+AGG 0.401991 2_2:-64418991 None:intergenic
CTTGGTACTAAATTCACAAA+AGG 0.404219 2_2:+64418367 Msa0264470:CDS
GTTGAAGAGCATAGGAATCT+TGG 0.409119 2_2:-64415515 None:intergenic
GTTGAAGAGCATAGGAATCT+TGG 0.409119 2_2:-64417880 None:intergenic
GATGTTTGAGGAAGACGTTT+GGG 0.411150 2_2:+64419317 Msa0264470:CDS
AGAAGGGAAAAGGGTTGGAT+TGG 0.418599 2_2:-64415856 None:intergenic
GAGTGAATGAAACAGGTCTT+AGG 0.420195 2_2:+64418938 Msa0264470:CDS
TATAATTGGATCAAGTTCAT+AGG 0.422425 2_2:-64415754 None:intergenic
GTAGGAGGAAGAGGAGGAAG+AGG 0.424502 2_2:-64417941 None:intergenic
GAAAGAGCATTATTGAAAGA+AGG 0.427388 2_2:-64418217 None:intergenic
GAAAGAGCATTATTGAAAGA+AGG 0.427798 2_2:-64415873 None:intergenic
GAGTATTTGGGAGTAGAAAA+GGG 0.432472 2_2:-64418144 None:intergenic
TTGGAGAGAGTGAATGAAAC+AGG 0.438708 2_2:+64418931 Msa0264470:CDS
ATGAGGAAGTTGTGGATTTG+AGG 0.441434 2_2:+64418467 Msa0264470:CDS
GAGGGGATGGAAGAGTAGTT+GGG 0.441888 2_2:-64415812 None:intergenic
AACTATAATCACTGAAGTTA+GGG 0.442010 2_2:-64418054 None:intergenic
AAAGAGCATTATTGAAAGAA+GGG 0.443472 2_2:-64415872 None:intergenic
AAAGAGCATTATTGAAAGAA+GGG 0.443472 2_2:-64418216 None:intergenic
AGACGAAGAAGAGGAAGAAG+AGG 0.446816 2_2:-64415631 None:intergenic
CTCTTACTTTGTTCATGGAG+TGG 0.447982 2_2:-64417683 None:intergenic
TGATGTTTGAGGAAGACGTT+TGG 0.448987 2_2:+64419316 Msa0264470:CDS
AAGACACATTCTTCTCCAAC+AGG 0.450645 2_2:+64418565 Msa0264470:CDS
TTGAAAGAAGGGAAAAGGGT+TGG 0.454471 2_2:-64415861 None:intergenic
GAAGCACGTTTAGCAGGAAC+TGG 0.455780 2_2:+64418631 Msa0264470:CDS
ATTCTAGGCACATGGGAAAT+GGG 0.456200 2_2:+64419415 Msa0264470:CDS
GGTTATGGTTTCAAGTGGCT+TGG 0.456727 2_2:+64418832 Msa0264470:CDS
CTTTGAGTTCCATCACCTGT+TGG 0.456885 2_2:-64418580 None:intergenic
TATCCCTGTGATCCTCAACT+TGG 0.460979 2_2:-64418893 None:intergenic
GCAGATTATAAATAAATTGA+AGG 0.461295 2_2:+64419461 Msa0264470:CDS
TAGGAGAAAGAGAAGTTATG+AGG 0.463600 2_2:+64416091 Msa0264470:CDS
AGAATAAGAAGACGAAGAAG+AGG 0.467187 2_2:-64415640 None:intergenic
TCCTGTTAGGTTGATGTTTG+AGG 0.467599 2_2:+64419305 Msa0264470:CDS
AAACTATAATCACTGAAGCT+AGG 0.467624 2_2:-64415711 None:intergenic
GCAGCAGCAGTAGGAGGAAG+AGG 0.470349 2_2:-64417950 None:intergenic
GAGTAGTTGGGAGTAGAAAA+GGG 0.473309 2_2:-64415800 None:intergenic
ATTATTGAAAGAAGGGAAAA+GGG 0.478667 2_2:-64415865 None:intergenic
GTCTTACTTTGTTCATGGTG+TGG 0.478686 2_2:-64415318 None:intergenic
GAAGAGTCTTCCAATGCTCT+TGG 0.479029 2_2:+64417803 Msa0264470:CDS
TCCGAAACAACTGTCTCATC+AGG 0.480396 2_2:-64419105 None:intergenic
TGCCAATGCATATATGTCTC+TGG 0.481671 2_2:-64419393 None:intergenic
ACTCTTCATGCTCATTGTGT+TGG 0.485254 2_2:-64415422 None:intergenic
CCACCCAAGTTGAGGATCAC+AGG 0.486139 2_2:+64418889 Msa0264470:CDS
GCAGTAGCAGCAGTAGGAGG+AGG 0.489711 2_2:-64415573 None:intergenic
GACGAAGAAGAGGAAGAAGA+GGG 0.495930 2_2:-64415630 None:intergenic
GCAGCAGCAGTAGCAGCAGT+AGG 0.498059 2_2:-64415579 None:intergenic
TATCGTTTATCGAAAAGATC+TGG 0.498438 2_2:+64418862 Msa0264470:CDS
ACTCTTCATGATCATTGTGT+TGG 0.498453 2_2:-64417787 None:intergenic
GCACTTGAAGCACGTTTAGC+AGG 0.499951 2_2:+64418625 Msa0264470:CDS
GAAGAATTATCAAACAGATT+CGG 0.502364 2_2:+64416062 Msa0264470:CDS
TTGAATGTGAGGATCCTGTT+AGG 0.507367 2_2:+64419292 Msa0264470:CDS
AAAATTGGATGCTACAAGGT+TGG 0.509330 2_2:+64419535 Msa0264470:CDS
TTGGTACTAAATTCACAAAA+GGG 0.510419 2_2:+64418368 Msa0264470:CDS
AACCCTATCACAGCCTTCAC+AGG 0.513850 2_2:-64419364 None:intergenic
GTTGATATAACCAAGAGCAT+TGG 0.514520 2_2:-64415448 None:intergenic
GTTGATATAACCAAGAGCAT+TGG 0.514520 2_2:-64417813 None:intergenic
CATTCTTCTCCAACAGGTGA+TGG 0.515669 2_2:+64418571 Msa0264470:CDS
AACTATAATCACTGAAGCTA+GGG 0.515859 2_2:-64415710 None:intergenic
TCGATGGACAAAAGAGTTGT+TGG 0.516333 2_2:+64415294 None:intergenic
GAAGAGTCATCCAATGCTCT+TGG 0.522768 2_2:+64415438 Msa0264470:CDS
TTATATCAACCAATTTCTCA+TGG 0.530130 2_2:+64415461 Msa0264470:CDS
TTATATCAACCAATTTCTCA+TGG 0.530130 2_2:+64417826 Msa0264470:CDS
AAGAAGTTATGAGGAAGTTG+TGG 0.533441 2_2:+64418459 Msa0264470:CDS
AAAATGTGATTAGGAGGGGA+TGG 0.534633 2_2:-64415825 None:intergenic
CTAGTTGTGAAGGTCTATGC+TGG 0.539897 2_2:-64419594 None:intergenic
CTTGGTACTAAAGTCGCAAA+AGG 0.541250 2_2:+64416008 Msa0264470:CDS
CAATAATGCTCTTTCTCACG+AGG 0.541791 2_2:+64415881 Msa0264470:CDS
GGCCAGAGACATATATGCAT+TGG 0.541953 2_2:+64419391 Msa0264470:CDS
TACTCATGCTGGATGGAAGA+AGG 0.547361 2_2:-64418004 None:intergenic
GGCATGCTGATGAATAAACT+TGG 0.547453 2_2:-64418717 None:intergenic
TTGGATGCTACAAGGTTGGA+AGG 0.548278 2_2:+64419539 Msa0264470:CDS
TAGGAGACAAAGAAGTTATG+AGG 0.549337 2_2:+64418450 Msa0264470:CDS
CAGATTATAAATAAATTGAA+GGG 0.550775 2_2:+64419462 Msa0264470:CDS
ATTCATAGCATTGTTAATGG+TGG 0.554304 2_2:+64419183 Msa0264470:CDS
ATCCCTGTGATCCTCAACTT+GGG 0.558025 2_2:-64418892 None:intergenic
AGGTGATGGAACTCAAAGAT+TGG 0.559955 2_2:+64418585 Msa0264470:CDS
AGCCTGTGAAGGCTGTGATA+GGG 0.569110 2_2:+64419362 Msa0264470:CDS
GCAGCAGTAGCAGCAGTAGG+AGG 0.573244 2_2:-64415576 None:intergenic
ATGTTTGAGGAAGACGTTTG+GGG 0.578234 2_2:+64419318 Msa0264470:CDS
AAATCCAAAACAGCACCTCT+CGG 0.585044 2_2:-64419132 None:intergenic
AATTTGTTATAGATGGACAA+AGG 0.588146 2_2:+64417650 Msa0264470:intron
GCATTGGCATTCTAGGCACA+TGG 0.590416 2_2:+64419407 Msa0264470:CDS
TATATGTGGATCAAGTTCAA+AGG 0.594302 2_2:-64418098 None:intergenic
GAATTATCAAACAGATTCGG+TGG 0.598197 2_2:+64416065 Msa0264470:CDS
CTCAGTAAGTTGAAGAGCAT+AGG 0.600415 2_2:-64415523 None:intergenic
CTCAGTAAGTTGAAGAGCAT+AGG 0.600415 2_2:-64417888 None:intergenic
CACCCAAGTTGAGGATCACA+GGG 0.601694 2_2:+64418890 Msa0264470:CDS
CGTTTATCGAAAAGATCTGG+TGG 0.602238 2_2:+64418865 Msa0264470:CDS
AGAAGAGGGAAGAGTAGCGG+CGG 0.604567 2_2:-64415616 None:intergenic
CAGTAGCAGCAGTAGGAGGA+GGG 0.605945 2_2:-64415572 None:intergenic
AAGGAAAATTGGATGCTACA+AGG 0.606455 2_2:+64419531 Msa0264470:CDS
CCTGTGATCCTCAACTTGGG+TGG 0.609546 2_2:-64418889 None:intergenic
GTGTTGGTTATGGTTTCAAG+TGG 0.612035 2_2:+64418827 Msa0264470:CDS
GGAAGAAGAGGGAAGAGTAG+CGG 0.613439 2_2:-64415619 None:intergenic
GAGGGTGAGAAATAACATCA+TGG 0.614205 2_2:-64415554 None:intergenic
TGTTTGAGGAAGACGTTTGG+GGG 0.623857 2_2:+64419319 Msa0264470:CDS
TAGTTGTATGAGTCTATATG+TGG 0.625308 2_2:-64418112 None:intergenic
GCAGCAGTAGGAGGAAGAGG+AGG 0.633732 2_2:-64417947 None:intergenic
AGTGATTATAGTTTCAGCAG+TGG 0.635543 2_2:+64415720 Msa0264470:CDS
TGGATGCTACAAGGTTGGAA+GGG 0.641743 2_2:+64419540 Msa0264470:CDS
CATTGGCATTCTAGGCACAT+GGG 0.643663 2_2:+64419408 Msa0264470:CDS
GTAAGCTTTGACCATATCTG+CGG 0.645774 2_2:-64418695 None:intergenic
GTTTATAAAACCAAGAACAG+TGG 0.646066 2_2:-64417717 None:intergenic
AAGGCTGTGATAGGGTTCAG+AGG 0.656517 2_2:+64419370 Msa0264470:CDS
CTGGTGGGCCACCCAAGTTG+AGG 0.658509 2_2:+64418881 Msa0264470:CDS
ATGTGGATCAAGTTCAAAGG+AGG 0.665342 2_2:-64418095 None:intergenic
ATGAATGTTATAGCCTGTGA+AGG 0.667099 2_2:+64419351 Msa0264470:CDS
TTGTTGATACTCATGCTGGA+TGG 0.669072 2_2:-64418011 None:intergenic
TAACAACAATGTTGAATGTG+AGG 0.669855 2_2:+64419281 Msa0264470:CDS
AAGTTATTAGGAGAAAATGG+AGG 0.670375 2_2:-64418166 None:intergenic
GTTTATCGAAAAGATCTGGT+GGG 0.689733 2_2:+64418866 Msa0264470:CDS
GTTGTTTCGGAGAATCCGAG+AGG 0.745211 2_2:+64419117 Msa0264470:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTATAACAACAAAAATTTT+GGG - chr2_2:64417633-64417652 None:intergenic 10.0%
!!! AATTATAACAACAAAAATTT+TGG - chr2_2:64417634-64417653 None:intergenic 10.0%
!!! TTTTATAAACAAATTTCTCA+TGG + chr2_2:64415365-64415384 Msa0264470:CDS 15.0%
!! TTATTTGATAAATTACTTCT+TGG + chr2_2:64415990-64416009 Msa0264470:CDS 15.0%
!!! TATTTTAATCTTTTTGTGTT+TGG + chr2_2:64417046-64417065 Msa0264470:intron 15.0%
!!! AGGTTTCTTAAATAAATTTT+TGG - chr2_2:64417213-64417232 None:intergenic 15.0%
!! TATTATATGTAAAAGTTCTT+AGG - chr2_2:64417233-64417252 None:intergenic 15.0%
!!! AAGAACTTTTACATATAATA+AGG + chr2_2:64417233-64417252 Msa0264470:intron 15.0%
!! AAAAAGAATGAAGAAATATA+TGG + chr2_2:64417337-64417356 Msa0264470:intron 15.0%
!!! TTGTTATAATTTGTTATAGA+TGG + chr2_2:64417643-64417662 Msa0264470:intron 15.0%
!!! TTTTATAAACAAATTTCTCA+TGG + chr2_2:64415365-64415384 Msa0264470:CDS 15.0%
!! ATGCTATGAATAAATATATT+TGG - chr2_2:64419174-64419193 None:intergenic 15.0%
!! CAGATTATAAATAAATTGAA+GGG + chr2_2:64419462-64419481 Msa0264470:CDS 15.0%
!!! TCTAAAAAGTATAGCTTTTA+GGG - chr2_2:64416314-64416333 None:intergenic 20.0%
!!! TTCTAAAAAGTATAGCTTTT+AGG - chr2_2:64416315-64416334 None:intergenic 20.0%
!!! AAAAGATGTGACTATATTTT+AGG - chr2_2:64416533-64416552 None:intergenic 20.0%
!!! AAAATATAGTCACATCTTTT+AGG + chr2_2:64416533-64416552 Msa0264470:intron 20.0%
!! TGGTTTCCTTAAATAATATA+AGG + chr2_2:64416618-64416637 Msa0264470:intron 20.0%
!!! TTCCTTTAATTTTTTCTCTT+AGG + chr2_2:64416967-64416986 Msa0264470:intron 20.0%
!! ATCCTAAGAGAAAAAATTAA+AGG - chr2_2:64416972-64416991 None:intergenic 20.0%
!!! ATGATATTGGTTAATATAAG+TGG - chr2_2:64417559-64417578 None:intergenic 20.0%
!!! TTTTGATTTGTGTATGATAT+TGG - chr2_2:64417572-64417591 None:intergenic 20.0%
!! TGAAATATCATTACAAGAAA+TGG - chr2_2:64418518-64418537 None:intergenic 20.0%
!!! TCTTAATCTGGTTTAATATT+TGG - chr2_2:64418549-64418568 None:intergenic 20.0%
!!! TAATTGCAAGTTTTTCAAAA+GGG - chr2_2:64418741-64418760 None:intergenic 20.0%
!!! ATAATTGCAAGTTTTTCAAA+AGG - chr2_2:64418742-64418761 None:intergenic 20.0%
!! TTTATTCATAGCATTGTTAA+TGG + chr2_2:64419180-64419199 Msa0264470:CDS 20.0%
!! GCAGATTATAAATAAATTGA+AGG + chr2_2:64419461-64419480 Msa0264470:CDS 20.0%
! TTATATCAACCAATTTCTCA+TGG + chr2_2:64415461-64415480 Msa0264470:CDS 25.0%
! TATAATTGGATCAAGTTCAT+AGG - chr2_2:64415757-64415776 None:intergenic 25.0%
! TAGTTGTATGAGTCTATAAT+TGG - chr2_2:64415771-64415790 None:intergenic 25.0%
! GATTGAGAAAAAATGTGATT+AGG - chr2_2:64415837-64415856 None:intergenic 25.0%
! ATTATTGAAAGAAGGGAAAA+GGG - chr2_2:64415868-64415887 None:intergenic 25.0%
!!! AAAGAGCATTATTGAAAGAA+GGG - chr2_2:64415875-64415894 None:intergenic 25.0%
! GAAGAATTATCAAACAGATT+CGG + chr2_2:64416062-64416081 Msa0264470:CDS 25.0%
!!! AGCTATACTTTTTAGAATGA+CGG + chr2_2:64416318-64416337 Msa0264470:intron 25.0%
!! CTTTTATACTGAAGCAATAT+GGG + chr2_2:64416373-64416392 Msa0264470:intron 25.0%
!! GGAAATATTTGAAGTTGTAT+TGG - chr2_2:64416512-64416531 None:intergenic 25.0%
! GTCAATAAGATAAGGAAATA+TGG + chr2_2:64416598-64416617 Msa0264470:intron 25.0%
!! TCTCAAAAAAGTAAAGTGTT+GGG - chr2_2:64416806-64416825 None:intergenic 25.0%
!! AAATTTAAGTTCTCATCAAC+AGG + chr2_2:64417260-64417279 Msa0264470:intron 25.0%
!!! TTCATTCTTTTTTGTTGAAG+TGG - chr2_2:64417329-64417348 None:intergenic 25.0%
! GAATGAAGAAATATATGGTT+AGG + chr2_2:64417342-64417361 Msa0264470:intron 25.0%
! TCTTGAACCAATCAAATAAT+TGG + chr2_2:64417489-64417508 Msa0264470:intron 25.0%
!!! AATTTGTTATAGATGGACAA+AGG + chr2_2:64417650-64417669 Msa0264470:intron 25.0%
! TTATATCAACCAATTTCTCA+TGG + chr2_2:64415461-64415480 Msa0264470:CDS 25.0%
! AACTATAATCACTGAAGTTA+GGG - chr2_2:64418057-64418076 None:intergenic 25.0%
! AAACTATAATCACTGAAGTT+AGG - chr2_2:64418058-64418077 None:intergenic 25.0%
! ACAAAGTTATTAGGAGAAAA+TGG - chr2_2:64418172-64418191 None:intergenic 25.0%
! ATTATTGAAAGAAGGGAAAA+AGG - chr2_2:64415868-64415887 None:intergenic 25.0%
!!! AAAGAGCATTATTGAAAGAA+GGG - chr2_2:64415875-64415894 None:intergenic 25.0%
!! TTTGATAAAGTACTAGTTCT+TGG + chr2_2:64418349-64418368 Msa0264470:CDS 25.0%
! TTGGTACTAAATTCACAAAA+GGG + chr2_2:64418368-64418387 Msa0264470:CDS 25.0%
! GAATTATCAAACAGATTCTA+TGG + chr2_2:64418424-64418443 Msa0264470:CDS 25.0%
!! AGTCTTCACATTATAGATTT+TGG + chr2_2:64418805-64418824 Msa0264470:CDS 25.0%
! ACAATGGTATTGCAAAAAAT+TGG + chr2_2:64419001-64419020 Msa0264470:CDS 25.0%
! CAATGGTATTGCAAAAAATT+GGG + chr2_2:64419002-64419021 Msa0264470:CDS 25.0%
!!! AAGTTCAGGATTTTAAGATA+AGG + chr2_2:64419034-64419053 Msa0264470:CDS 25.0%
!!! TGTTTTGGATTTGATTAAGA+AGG + chr2_2:64419143-64419162 Msa0264470:CDS 25.0%
! TTAAACCTTGTCACAAAAAA+AGG - chr2_2:64419219-64419238 None:intergenic 25.0%
!!! TTTTTTTGTGACAAGGTTTA+AGG + chr2_2:64419218-64419237 Msa0264470:CDS 25.0%
!! TAATAGTGATTTTCTGTTTG+AGG + chr2_2:64419506-64419525 Msa0264470:CDS 25.0%
ATTCAGTCTTACTTTGTTCA+TGG - chr2_2:64415326-64415345 None:intergenic 30.0%
AATCTTCTTCCATGAGAAAT+TGG - chr2_2:64415473-64415492 None:intergenic 30.0%
AACTATAATCACTGAAGCTA+GGG - chr2_2:64415713-64415732 None:intergenic 30.0%
AAACTATAATCACTGAAGCT+AGG - chr2_2:64415714-64415733 None:intergenic 30.0%
AGAAAAAATGTGATTAGGAG+GGG - chr2_2:64415832-64415851 None:intergenic 30.0%
GAGAAAAAATGTGATTAGGA+GGG - chr2_2:64415833-64415852 None:intergenic 30.0%
TGAGAAAAAATGTGATTAGG+AGG - chr2_2:64415834-64415853 None:intergenic 30.0%
CATTATTGAAAGAAGGGAAA+AGG - chr2_2:64415869-64415888 None:intergenic 30.0%
!! GAAAGAGCATTATTGAAAGA+AGG - chr2_2:64415876-64415895 None:intergenic 30.0%
AAGACCTAATTGAGTATTCA+AGG - chr2_2:64416344-64416363 None:intergenic 30.0%
CCATATTGCTTCAGTATAAA+AGG - chr2_2:64416375-64416394 None:intergenic 30.0%
! CCTTTTATACTGAAGCAATA+TGG + chr2_2:64416372-64416391 Msa0264470:intron 30.0%
TAAAGATGTTCCTACATTGT+AGG - chr2_2:64416482-64416501 None:intergenic 30.0%
TACAATGTAGGAACATCTTT+AGG + chr2_2:64416481-64416500 Msa0264470:intron 30.0%
ACAATATTCCAACATGTTTC+AGG - chr2_2:64416727-64416746 None:intergenic 30.0%
AATATCCATGTTTGTGTTGT+TGG - chr2_2:64416752-64416771 None:intergenic 30.0%
!! AAAGTGTTGGGTATTTGTAT+TGG - chr2_2:64416794-64416813 None:intergenic 30.0%
CTCTCAAAAAAGTAAAGTGT+TGG - chr2_2:64416807-64416826 None:intergenic 30.0%
AAATCAGAGGATGTAACTTT+AGG + chr2_2:64416851-64416870 Msa0264470:intron 30.0%
AATCAGAGGATGTAACTTTA+GGG + chr2_2:64416852-64416871 Msa0264470:intron 30.0%
AAAACAGTTGTAAACACCTT+GGG + chr2_2:64416910-64416929 Msa0264470:intron 30.0%
TAATAAAATCTCACTACCCA+AGG - chr2_2:64416929-64416948 None:intergenic 30.0%
! TTGGGTAGTGAGATTTTATT+AGG + chr2_2:64416928-64416947 Msa0264470:intron 30.0%
! TGGGTAGTGAGATTTTATTA+GGG + chr2_2:64416929-64416948 Msa0264470:intron 30.0%
ATGACTTGAAAAATAGGTAG+AGG + chr2_2:64417007-64417026 Msa0264470:intron 30.0%
AAAACTAGCAATGGATAACA+AGG - chr2_2:64417114-64417133 None:intergenic 30.0%
AAACGATAGAAAACTAGCAA+TGG - chr2_2:64417123-64417142 None:intergenic 30.0%
TTGTTGAAGTGGAGATAATT+TGG - chr2_2:64417318-64417337 None:intergenic 30.0%
ATGGACAAAGCAAATAGTTT+GGG - chr2_2:64417452-64417471 None:intergenic 30.0%
AATGGACAAAGCAAATAGTT+TGG - chr2_2:64417453-64417472 None:intergenic 30.0%
ACCAATCAAATAATTGGACA+CGG + chr2_2:64417495-64417514 Msa0264470:intron 30.0%
TGGCTGAATTCTTTCATATT+TGG - chr2_2:64417539-64417558 None:intergenic 30.0%
TGGGTTATATCAAATTAGTG+TGG - chr2_2:64417614-64417633 None:intergenic 30.0%
ATTCACTCTTACTTTGTTCA+TGG - chr2_2:64417691-64417710 None:intergenic 30.0%
GTTTATAAAACCAAGAACAG+TGG - chr2_2:64415355-64415374 None:intergenic 30.0%
AATCTTCTTCCATGAGAAAT+TGG - chr2_2:64415473-64415492 None:intergenic 30.0%
TATATGTGGATCAAGTTCAA+AGG - chr2_2:64418101-64418120 None:intergenic 30.0%
TAGTTGTATGAGTCTATATG+TGG - chr2_2:64418115-64418134 None:intergenic 30.0%
AAGTTATTAGGAGAAAATGG+AGG - chr2_2:64418169-64418188 None:intergenic 30.0%
GATTGGGAAACAAAGTTATT+AGG - chr2_2:64418181-64418200 None:intergenic 30.0%
GAAAAAGGTTGAAGTAGATT+GGG - chr2_2:64418197-64418216 None:intergenic 30.0%
!! GAAAGAGCATTATTGAAAGA+AGG - chr2_2:64415876-64415895 None:intergenic 30.0%
TTTCTCAATGTGTCACAAAA+TGG + chr2_2:64418280-64418299 Msa0264470:CDS 30.0%
CTTGGTACTAAATTCACAAA+AGG + chr2_2:64418367-64418386 Msa0264470:CDS 30.0%
! TTGATAATTCTTCATTCTGC+TGG - chr2_2:64416055-64416074 None:intergenic 30.0%
!! CACATTATAGATTTTGGTGT+TGG + chr2_2:64418811-64418830 Msa0264470:CDS 30.0%
!!! ATAGATTTTGGTGTTGGTTA+TGG + chr2_2:64418817-64418836 Msa0264470:CDS 30.0%
TATCGTTTATCGAAAAGATC+TGG + chr2_2:64418862-64418881 Msa0264470:CDS 30.0%
GATAGATCTTCCAAATTCAT+TGG + chr2_2:64418912-64418931 Msa0264470:CDS 30.0%
!! AATGTTCCTTTTGAGTACAA+TGG + chr2_2:64418985-64419004 Msa0264470:CDS 30.0%
ATTCATAGCATTGTTAATGG+TGG + chr2_2:64419183-64419202 Msa0264470:CDS 30.0%
TAACAACAATGTTGAATGTG+AGG + chr2_2:64419281-64419300 Msa0264470:CDS 30.0%
GAATTCTCTTCAACTGTTCT+TGG + chr2_2:64415342-64415361 Msa0264470:CDS 35.0%
! GTTGATATAACCAAGAGCAT+TGG - chr2_2:64415451-64415470 None:intergenic 35.0%
AGAATAAGAAGACGAAGAAG+AGG - chr2_2:64415643-64415662 None:intergenic 35.0%
AGTGATTATAGTTTCAGCAG+TGG + chr2_2:64415720-64415739 Msa0264470:CDS 35.0%
!!! TGAAAGCTTGTGTTTTGAAG+TGG - chr2_2:64416035-64416054 None:intergenic 35.0%
GAATTATCAAACAGATTCGG+TGG + chr2_2:64416065-64416084 Msa0264470:CDS 35.0%
TAGGAGAAAGAGAAGTTATG+AGG + chr2_2:64416091-64416110 Msa0264470:CDS 35.0%
ACAGTACTAAACTTGCATCA+AGG + chr2_2:64416256-64416275 Msa0264470:intron 35.0%
ACACGAATCAGAGACAAATA+TGG + chr2_2:64416451-64416470 Msa0264470:intron 35.0%
TATGGAAAGTCCTACAATGT+AGG + chr2_2:64416469-64416488 Msa0264470:intron 35.0%
GTTGCTTGGTCAATAAGATA+AGG + chr2_2:64416590-64416609 Msa0264470:intron 35.0%
TGTATCCAACAACACAAACA+TGG + chr2_2:64416744-64416763 Msa0264470:intron 35.0%
AGTTTCAAGAGAGAGAAACT+TGG + chr2_2:64416827-64416846 Msa0264470:intron 35.0%
AGAGAAACTTGGTAAATCAG+AGG + chr2_2:64416838-64416857 Msa0264470:intron 35.0%
GAAAACAGTTGTAAACACCT+TGG + chr2_2:64416909-64416928 Msa0264470:intron 35.0%
! GGGTAGTGAGATTTTATTAG+GGG + chr2_2:64416930-64416949 Msa0264470:intron 35.0%
! GGTAGTGAGATTTTATTAGG+GGG + chr2_2:64416931-64416950 Msa0264470:intron 35.0%
GTGTGGATGACTTGAAAAAT+AGG + chr2_2:64417001-64417020 Msa0264470:intron 35.0%
ACTTGAAAAATAGGTAGAGG+AGG + chr2_2:64417010-64417029 Msa0264470:intron 35.0%
CTTGAAAAATAGGTAGAGGA+GGG + chr2_2:64417011-64417030 Msa0264470:intron 35.0%
TTGAAAAATAGGTAGAGGAG+GGG + chr2_2:64417012-64417031 Msa0264470:intron 35.0%
AACGCATAACACAAACACAA+AGG - chr2_2:64417073-64417092 None:intergenic 35.0%
!!! GCTAGTTTTCTATCGTTTTG+TGG + chr2_2:64417125-64417144 Msa0264470:intron 35.0%
! TGTGTGGCAAAGATTCATTT+TGG - chr2_2:64417382-64417401 None:intergenic 35.0%
ACCGTGTCCAATTATTTGAT+TGG - chr2_2:64417499-64417518 None:intergenic 35.0%
ACTCTTCATGATCATTGTGT+TGG - chr2_2:64417790-64417809 None:intergenic 35.0%
! GTTGATATAACCAAGAGCAT+TGG - chr2_2:64415451-64415470 None:intergenic 35.0%
!!! AGTTTTGTTGATACTCATGC+TGG - chr2_2:64418018-64418037 None:intergenic 35.0%
GAGTATTTGGGAGTAGAAAA+GGG - chr2_2:64418147-64418166 None:intergenic 35.0%
!! GAGAAAATGGAGGAGTATTT+GGG - chr2_2:64418159-64418178 None:intergenic 35.0%
GGAAAAAGGTTGAAGTAGAT+TGG - chr2_2:64418198-64418217 None:intergenic 35.0%
CAAACAGATTCTATGGTTCT+AGG + chr2_2:64418431-64418450 Msa0264470:CDS 35.0%
TAGGAGACAAAGAAGTTATG+AGG + chr2_2:64418450-64418469 Msa0264470:CDS 35.0%
AAGAAGTTATGAGGAAGTTG+TGG + chr2_2:64418459-64418478 Msa0264470:CDS 35.0%
GAGAAGAATGTGTCTTAATC+TGG - chr2_2:64418561-64418580 None:intergenic 35.0%
CAAAGATTGGCACATTTCTT+TGG + chr2_2:64418598-64418617 Msa0264470:CDS 35.0%
! GTGAAGACTTTCTGTTTCTT+TGG - chr2_2:64418794-64418813 None:intergenic 35.0%
GTTTATCGAAAAGATCTGGT+GGG + chr2_2:64418866-64418885 Msa0264470:CDS 35.0%
TTCACTCTCTCCAATGAATT+TGG - chr2_2:64418925-64418944 None:intergenic 35.0%
GCAATACCATTGTACTCAAA+AGG - chr2_2:64418994-64419013 None:intergenic 35.0%
!! TTGGGAAAGTATCAAAGTTC+AGG + chr2_2:64419020-64419039 Msa0264470:CDS 35.0%
!!! ATGCACCTTTTTTTGTGACA+AGG + chr2_2:64419211-64419230 Msa0264470:CDS 35.0%
ATGAATGTTATAGCCTGTGA+AGG + chr2_2:64419351-64419370 Msa0264470:CDS 35.0%
! CATATATGCATTGGCATTCT+AGG + chr2_2:64419400-64419419 Msa0264470:CDS 35.0%
!! TGGGTTTAGATCTTTGAAAC+TGG + chr2_2:64419434-64419453 Msa0264470:CDS 35.0%
!! TGATTTTCTGTTTGAGGTGA+AGG + chr2_2:64419512-64419531 Msa0264470:CDS 35.0%
! TGTTTGAGGTGAAGGAAAAT+TGG + chr2_2:64419520-64419539 Msa0264470:CDS 35.0%
AAGGAAAATTGGATGCTACA+AGG + chr2_2:64419531-64419550 Msa0264470:CDS 35.0%
AAAATTGGATGCTACAAGGT+TGG + chr2_2:64419535-64419554 Msa0264470:CDS 35.0%
TGGAAGGGTAGAATTCTATT+TGG + chr2_2:64419555-64419574 Msa0264470:CDS 35.0%
!! TTCTATTTGGTTCTTCTTGC+TGG + chr2_2:64419568-64419587 Msa0264470:CDS 35.0%
!! TCTATTTGGTTCTTCTTGCT+GGG + chr2_2:64419569-64419588 Msa0264470:CDS 35.0%
GTCTTACTTTGTTCATGGTG+TGG - chr2_2:64415321-64415340 None:intergenic 40.0%
ACTCTTCATGCTCATTGTGT+TGG - chr2_2:64415425-64415444 None:intergenic 40.0%
! GTTGAAGAGCATAGGAATCT+TGG - chr2_2:64415518-64415537 None:intergenic 40.0%
! CTCAGTAAGTTGAAGAGCAT+AGG - chr2_2:64415526-64415545 None:intergenic 40.0%
GAGGGTGAGAAATAACATCA+TGG - chr2_2:64415557-64415576 None:intergenic 40.0%
!! AGTTGTAGTTTTGCTGATGC+TGG - chr2_2:64415680-64415699 None:intergenic 40.0%
GAGTAGTTGGGAGTAGAAAA+GGG - chr2_2:64415803-64415822 None:intergenic 40.0%
AGAGTAGTTGGGAGTAGAAA+AGG - chr2_2:64415804-64415823 None:intergenic 40.0%
AAAATGTGATTAGGAGGGGA+TGG - chr2_2:64415828-64415847 None:intergenic 40.0%
TTGAAAGAAGGGAAAAGGGT+TGG - chr2_2:64415864-64415883 None:intergenic 40.0%
CAATAATGCTCTTTCTCACG+AGG + chr2_2:64415881-64415900 Msa0264470:CDS 40.0%
CTTGGTACTAAAGTCGCAAA+AGG + chr2_2:64416008-64416027 Msa0264470:CDS 40.0%
!!! CAAACAGATTCGGTGGTTTT+AGG + chr2_2:64416072-64416091 Msa0264470:CDS 40.0%
GAGAAGTTATGAGGAAGTTG+TGG + chr2_2:64416100-64416119 Msa0264470:intron 40.0%
ATGAGGAAGTTGTGGATTTC+AGG + chr2_2:64416108-64416127 Msa0264470:intron 40.0%
ACGGCCTTGAATACTCAATT+AGG + chr2_2:64416337-64416356 Msa0264470:intron 40.0%
TATGGGCCATTTAACCTGTT+TGG + chr2_2:64416390-64416409 Msa0264470:intron 40.0%
ATGGGCCATTTAACCTGTTT+GGG + chr2_2:64416391-64416410 Msa0264470:intron 40.0%
TGAAGCCCAAACAGGTTAAA+TGG - chr2_2:64416399-64416418 None:intergenic 40.0%
CGCGCGCCTTATATTATTTA+AGG - chr2_2:64416627-64416646 None:intergenic 40.0%
GTACAAACAGGCATGATACA+AGG + chr2_2:64416646-64416665 Msa0264470:intron 40.0%
!! ACTTTAGGGTTGAGTGTCTT+TGG + chr2_2:64416866-64416885 Msa0264470:intron 40.0%
TCTTTGGTGAGAGTTTCTCT+TGG + chr2_2:64416882-64416901 Msa0264470:intron 40.0%
CTTTGGTGAGAGTTTCTCTT+GGG + chr2_2:64416883-64416902 Msa0264470:intron 40.0%
! AACCTTTGTTGTGTCATGTG+TGG - chr2_2:64417398-64417417 None:intergenic 40.0%
CTCTTACTTTGTTCATGGAG+TGG - chr2_2:64417686-64417705 None:intergenic 40.0%
GAATTCACTTCCACTGTTCT+TGG + chr2_2:64417707-64417726 Msa0264470:CDS 40.0%
! GTTGAAGAGCATAGGAATCT+TGG - chr2_2:64415518-64415537 None:intergenic 40.0%
! CTCAGTAAGTTGAAGAGCAT+AGG - chr2_2:64415526-64415545 None:intergenic 40.0%
TTGTTGATACTCATGCTGGA+TGG - chr2_2:64418014-64418033 None:intergenic 40.0%
ATGTGGATCAAGTTCAAAGG+AGG - chr2_2:64418098-64418117 None:intergenic 40.0%
! GGAGTATTTGGGAGTAGAAA+AGG - chr2_2:64418148-64418167 None:intergenic 40.0%
!! GGAGAAAATGGAGGAGTATT+TGG - chr2_2:64418160-64418179 None:intergenic 40.0%
!!! TGGAAGCTTGTGTTTTGAAG+TGG - chr2_2:64418394-64418413 None:intergenic 40.0%
ATGAGGAAGTTGTGGATTTG+AGG + chr2_2:64418467-64418486 Msa0264470:CDS 40.0%
AAGACACATTCTTCTCCAAC+AGG + chr2_2:64418565-64418584 Msa0264470:CDS 40.0%
!! AGGTGATGGAACTCAAAGAT+TGG + chr2_2:64418585-64418604 Msa0264470:CDS 40.0%
!!! ATCTGCGGCAGATTTTTTCT+TGG - chr2_2:64418683-64418702 None:intergenic 40.0%
GAAAAAATCTGCCGCAGATA+TGG + chr2_2:64418684-64418703 Msa0264470:CDS 40.0%
! GTAAGCTTTGACCATATCTG+CGG - chr2_2:64418698-64418717 None:intergenic 40.0%
GGCATGCTGATGAATAAACT+TGG - chr2_2:64418720-64418739 None:intergenic 40.0%
!! GTGTTGGTTATGGTTTCAAG+TGG + chr2_2:64418827-64418846 Msa0264470:CDS 40.0%
CGTTTATCGAAAAGATCTGG+TGG + chr2_2:64418865-64418884 Msa0264470:CDS 40.0%
TTGGAGAGAGTGAATGAAAC+AGG + chr2_2:64418931-64418950 Msa0264470:CDS 40.0%
GAGTGAATGAAACAGGTCTT+AGG + chr2_2:64418938-64418957 Msa0264470:CDS 40.0%
ACCTGATGAGACAGTTGTTT+CGG + chr2_2:64419104-64419123 Msa0264470:CDS 40.0%
AAATCCAAAACAGCACCTCT+CGG - chr2_2:64419135-64419154 None:intergenic 40.0%
TTGAATGTGAGGATCCTGTT+AGG + chr2_2:64419292-64419311 Msa0264470:CDS 40.0%
!! TCCTGTTAGGTTGATGTTTG+AGG + chr2_2:64419305-64419324 Msa0264470:CDS 40.0%
TCCTCAAACATCAACCTAAC+AGG - chr2_2:64419309-64419328 None:intergenic 40.0%
TGATGTTTGAGGAAGACGTT+TGG + chr2_2:64419316-64419335 Msa0264470:CDS 40.0%
GATGTTTGAGGAAGACGTTT+GGG + chr2_2:64419317-64419336 Msa0264470:CDS 40.0%
ATGTTTGAGGAAGACGTTTG+GGG + chr2_2:64419318-64419337 Msa0264470:CDS 40.0%
TGCCAATGCATATATGTCTC+TGG - chr2_2:64419396-64419415 None:intergenic 40.0%
ATTCTAGGCACATGGGAAAT+GGG + chr2_2:64419415-64419434 Msa0264470:CDS 40.0%
GAAGAGTCATCCAATGCTCT+TGG + chr2_2:64415438-64415457 Msa0264470:CDS 45.0%
GACGAAGAAGAGGAAGAAGA+GGG - chr2_2:64415633-64415652 None:intergenic 45.0%
AGACGAAGAAGAGGAAGAAG+AGG - chr2_2:64415634-64415653 None:intergenic 45.0%
!! GTAGTTTTGCTGATGCTGGA+TGG - chr2_2:64415676-64415695 None:intergenic 45.0%
AGAAGGGAAAAGGGTTGGAT+TGG - chr2_2:64415859-64415878 None:intergenic 45.0%
!! CTTTGTTCTGAAGCCCAAAC+AGG - chr2_2:64416407-64416426 None:intergenic 45.0%
! TTCGTGTTTTGCAGATCAGC+TGG - chr2_2:64416438-64416457 None:intergenic 45.0%
GTAAAGATATGCGCGTTGCT+TGG + chr2_2:64416576-64416595 Msa0264470:intron 45.0%
ATTGAACCATGCCAGGATGT+TGG + chr2_2:64416672-64416691 Msa0264470:intron 45.0%
CAGGCATCTCTGTAAGATGT+TGG - chr2_2:64416708-64416727 None:intergenic 45.0%
CAGAGATGCCTGAAACATGT+TGG + chr2_2:64416716-64416735 Msa0264470:intron 45.0%
CTTAGGATCCATTGTGAGTG+TGG + chr2_2:64416984-64417003 Msa0264470:intron 45.0%
CAAGTCATCCACACTCACAA+TGG - chr2_2:64416995-64417014 None:intergenic 45.0%
TGCCACACATGACACAACAA+AGG + chr2_2:64417393-64417412 Msa0264470:intron 45.0%
GTTAGTCCACTTCTACTCAG+AGG + chr2_2:64417415-64417434 Msa0264470:intron 45.0%
AGCAAATAGTTTGGGCGTGT+GGG - chr2_2:64417444-64417463 None:intergenic 45.0%
AAGCAAATAGTTTGGGCGTG+TGG - chr2_2:64417445-64417464 None:intergenic 45.0%
!! GTCCATTTGCAGTGTTGACT+TGG + chr2_2:64417466-64417485 Msa0264470:intron 45.0%
GACCAAGTCAACACTGCAAA+TGG - chr2_2:64417471-64417490 None:intergenic 45.0%
GAAGAGTCTTCCAATGCTCT+TGG + chr2_2:64417803-64417822 Msa0264470:CDS 45.0%
TACTCATGCTGGATGGAAGA+AGG - chr2_2:64418007-64418026 None:intergenic 45.0%
CATTCTTCTCCAACAGGTGA+TGG + chr2_2:64418571-64418590 Msa0264470:CDS 45.0%
! CTTTGAGTTCCATCACCTGT+TGG - chr2_2:64418583-64418602 None:intergenic 45.0%
! GGTTATGGTTTCAAGTGGCT+TGG + chr2_2:64418832-64418851 Msa0264470:CDS 45.0%
ATCCCTGTGATCCTCAACTT+GGG - chr2_2:64418895-64418914 None:intergenic 45.0%
TATCCCTGTGATCCTCAACT+TGG - chr2_2:64418896-64418915 None:intergenic 45.0%
TCCGAAACAACTGTCTCATC+AGG - chr2_2:64419108-64419127 None:intergenic 45.0%
TGTTTGAGGAAGACGTTTGG+GGG + chr2_2:64419319-64419338 Msa0264470:CDS 45.0%
GGCCAGAGACATATATGCAT+TGG + chr2_2:64419391-64419410 Msa0264470:CDS 45.0%
CATTGGCATTCTAGGCACAT+GGG + chr2_2:64419408-64419427 Msa0264470:CDS 45.0%
CATTCTAGGCACATGGGAAA+TGG + chr2_2:64419414-64419433 Msa0264470:CDS 45.0%
TTGGATGCTACAAGGTTGGA+AGG + chr2_2:64419539-64419558 Msa0264470:CDS 45.0%
TGGATGCTACAAGGTTGGAA+GGG + chr2_2:64419540-64419559 Msa0264470:CDS 45.0%
GGAAGAAGAGGGAAGAGTAG+CGG - chr2_2:64415622-64415641 None:intergenic 50.0%
GAGGGGATGGAAGAGTAGTT+GGG - chr2_2:64415815-64415834 None:intergenic 50.0%
TATAAGGCGCGCGTACAAAC+AGG + chr2_2:64416634-64416653 Msa0264470:intron 50.0%
AAGGCACATTGAACCATGCC+AGG + chr2_2:64416665-64416684 Msa0264470:intron 50.0%
GATGTTCCAACATCCTGGCA+TGG - chr2_2:64416681-64416700 None:intergenic 50.0%
GACACGATGTTCCAACATCC+TGG - chr2_2:64416686-64416705 None:intergenic 50.0%
GGTGAGAGTTTCTCTTGGGT+TGG + chr2_2:64416887-64416906 Msa0264470:intron 50.0%
AAATAGTTTGGGCGTGTGGG+GGG - chr2_2:64417441-64417460 None:intergenic 50.0%
CAAATAGTTTGGGCGTGTGG+GGG - chr2_2:64417442-64417461 None:intergenic 50.0%
GCAAATAGTTTGGGCGTGTG+GGG - chr2_2:64417443-64417462 None:intergenic 50.0%
GCACTTGAAGCACGTTTAGC+AGG + chr2_2:64418625-64418644 Msa0264470:CDS 50.0%
GAAGCACGTTTAGCAGGAAC+TGG + chr2_2:64418631-64418650 Msa0264470:CDS 50.0%
! CACCCAAGTTGAGGATCACA+GGG + chr2_2:64418890-64418909 Msa0264470:CDS 50.0%
! GTTGTTTCGGAGAATCCGAG+AGG + chr2_2:64419117-64419136 Msa0264470:CDS 50.0%
!!! GAATCCGAGAGGTGCTGTTT+TGG + chr2_2:64419128-64419147 Msa0264470:CDS 50.0%
TAGCCTGTGAAGGCTGTGAT+AGG + chr2_2:64419361-64419380 Msa0264470:CDS 50.0%
AGCCTGTGAAGGCTGTGATA+GGG + chr2_2:64419362-64419381 Msa0264470:CDS 50.0%
AACCCTATCACAGCCTTCAC+AGG - chr2_2:64419367-64419386 None:intergenic 50.0%
AAGGCTGTGATAGGGTTCAG+AGG + chr2_2:64419370-64419389 Msa0264470:CDS 50.0%
! GCATTGGCATTCTAGGCACA+TGG + chr2_2:64419407-64419426 Msa0264470:CDS 50.0%
CAGTAGCAGCAGTAGGAGGA+GGG - chr2_2:64415575-64415594 None:intergenic 55.0%
AGAAGAGGGAAGAGTAGCGG+CGG - chr2_2:64415619-64415638 None:intergenic 55.0%
GGAGGGGATGGAAGAGTAGT+TGG - chr2_2:64415816-64415835 None:intergenic 55.0%
GTAGGAGGAAGAGGAGGAAG+AGG - chr2_2:64417944-64417963 None:intergenic 55.0%
CCTGTGATCCTCAACTTGGG+TGG - chr2_2:64418892-64418911 None:intergenic 55.0%
! CCACCCAAGTTGAGGATCAC+AGG + chr2_2:64418889-64418908 Msa0264470:CDS 55.0%
GCAGTAGCAGCAGTAGGAGG+AGG - chr2_2:64415576-64415595 None:intergenic 60.0%
GCAGCAGTAGCAGCAGTAGG+AGG - chr2_2:64415579-64415598 None:intergenic 60.0%
GCAGCAGCAGTAGCAGCAGT+AGG - chr2_2:64415582-64415601 None:intergenic 60.0%
GCAGCAGTAGGAGGAAGAGG+AGG - chr2_2:64417950-64417969 None:intergenic 60.0%
GCAGCAGCAGTAGGAGGAAG+AGG - chr2_2:64417953-64417972 None:intergenic 60.0%
ACAGCAGCAGCAGCAGCAGT+AGG - chr2_2:64417962-64417981 None:intergenic 60.0%
GGGGGGCCTCTGAGTAGAAG+TGG - chr2_2:64417424-64417443 None:intergenic 65.0%
GCAGCAGCAGCAGCAGTAGG+AGG - chr2_2:64417959-64417978 None:intergenic 65.0%
! CTGGTGGGCCACCCAAGTTG+AGG + chr2_2:64418881-64418900 Msa0264470:CDS 65.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 64415297 64419602 64415297 ID=Msa0264470;Name=Msa0264470
chr2_2 mRNA 64415297 64419602 64415297 ID=Msa0264470-mRNA-1;Parent=Msa0264470;Name=Msa0264470-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0|0|1|1|1|2|0|919
chr2_2 exon 64415297 64416115 64415297 ID=Msa0264470-mRNA-1:exon:20084;Parent=Msa0264470-mRNA-1
chr2_2 exon 64417662 64419602 64417662 ID=Msa0264470-mRNA-1:exon:20085;Parent=Msa0264470-mRNA-1
chr2_2 CDS 64415297 64416115 64415297 ID=Msa0264470-mRNA-1:cds;Parent=Msa0264470-mRNA-1
chr2_2 CDS 64417662 64419602 64417662 ID=Msa0264470-mRNA-1:cds;Parent=Msa0264470-mRNA-1
Gene Sequence

>Msa0264470

ATGGACAAAAGAGTTGTTGGTCCACACCATGAACAAAGTAAGACTGAATTCTCTTCAACTGTTCTTGGTTTTATAAACAAATTTCTCATGGAAGATGATGACATAGAAGAAGACTACAATATGTTCCAACACAATGAGCATGAAGAGTCATCCAATGCTCTTGGTTATATCAACCAATTTCTCATGGAAGAAGATTTTGAAAAAGAATACACTATGTTCCAAGATTCCTATGCTCTTCAACTTACTGAGAAATCATTCCATGATGTTATTTCTCACCCTCCTCCTACTGCTGCTACTGCTGCTGCTGCTGCTGCTGCTGCCGCCGCTACTCTTCCCTCTTCTTCCTCTTCTTCGTCTTCTTATTCTTCTTCTTCTTCCATCCAGCATCAGCAAAACTACAACTATGTGCATAGCCCTAGCTTCAGTGATTATAGTTTCAGCAGTGGTAGCACTAGCTCCTATGAACTTGATCCAATTATAGACTCATACAACTATAATAACAACCCTTTTCTACTCCCAACTACTCTTCCATCCCCTCCTAATCACATTTTTTCTCAATCCAATCCAACCCTTTTCCCTTCTTTCAATAATGCTCTTTCTCACGAGGTAATTAAGACAGAAAACTTTGAAGAAGAGCATTTTCTTAATGTGTCAAAACAAGTGAATGTAGATGATAACAGTGAGTTATCAGAATTATTTGATAAATTACTTCTTGGTACTAAAGTCGCAAAAGGTCCACTTCAAAACACAAGCTTTCAGCAGAATGAAGAATTATCAAACAGATTCGGTGGTTTTAGGAGAAAGAGAAGTTATGAGGAAATGGACAAAGGAGTTGTTGATCCACTCCATGAACAAAGTAAGAGTGAATTCACTTCCACTGTTCTTGGTTTTATAAACAAATTTCTCATGGAAGATGATGACATAGAAGAAGACTACAATATGTTCCAACACAATGATCATGAAGAGTCTTCCAATGCTCTTGGTTATATCAACCAATTTCTCATGGAAGAAGATTTTGAAAAAGAATACACTATGTTCCAAGATTCCTATGCTCTTCAACTTACTGAGAAATCATTTCATGATGTTATTGCTCACACTCCTCTTCCTCCTCTTCCTCCTACTGCTGCTGCTGCTGCTGTTACTGCTTTTACTACTACTCTTCCTTCTTCCATCCAGCATGAGTATCAACAAAACTACAACTTTGTGCATAGCCCTAACTTCAGTGATTATAGTTTAAGCAGTGATAGCACTACCTCCTTTGAACTTGATCCACATATAGACTCATACAACTACAATTATAACCCTTTTCTACTCCCAAATACTCCTCCATTTTCTCCTAATAACTTTGTTTCCCAATCTACTTCAACCTTTTTCCCTTCTTTCAATAATGCTCTTTCTCATGAGCTACTTCAGACAGAAAACTTTGAAGAAGAGCATTTTCTCAATGTGTCACAAAATGGATCAGAACAAGTGTATGTAGATGATAGCAATGAGTTATCAGAATTATTTGATAAAGTACTAGTTCTTGGTACTAAATTCACAAAAGGGCCACTTCAAAACACAAGCTTCCAGCAGAATGAAGAATTATCAAACAGATTCTATGGTTCTAGGAGACAAAGAAGTTATGAGGAAGTTGTGGATTTGAGGACACTATTGATGCTGTGTGCACAATCCATTTCTTGTAATGATATTTCAAATGCTAACCAAATATTAAACCAGATTAAGACACATTCTTCTCCAACAGGTGATGGAACTCAAAGATTGGCACATTTCTTTGGAAATGCACTTGAAGCACGTTTAGCAGGAACTGGTTCACATATCTATAGAGCTCTATCTTCCAAGAAAAAATCTGCCGCAGATATGGTCAAAGCTTACCAAGTTTATTCATCAGCATGCCCTTTTGAAAAACTTGCAATTATGTTCTCAAACGATGCAATTTTGAATGTAGCCAAAGAAACAGAAAGTCTTCACATTATAGATTTTGGTGTTGGTTATGGTTTCAAGTGGCTTGGTTTTATATATCGTTTATCGAAAAGATCTGGTGGGCCACCCAAGTTGAGGATCACAGGGATAGATCTTCCAAATTCATTGGAGAGAGTGAATGAAACAGGTCTTAGGCTTGCAAGTTATTGCAAACGTTTCAATGTTCCTTTTGAGTACAATGGTATTGCAAAAAATTGGGAAAGTATCAAAGTTCAGGATTTTAAGATAAGGAAAAATGAGTTTGTTGCTGTGACTTGTGTGTTTAAGTTTGAGAATCTACCTGATGAGACAGTTGTTTCGGAGAATCCGAGAGGTGCTGTTTTGGATTTGATTAAGAAGGCAAATCCAAATATATTTATTCATAGCATTGTTAATGGTGGTTATGATGCACCTTTTTTTGTGACAAGGTTTAAGGAAGCTGTTTTTTATTATTCAGCATTGTTTGATATGCTTGATAACAACAATGTTGAATGTGAGGATCCTGTTAGGTTGATGTTTGAGGAAGACGTTTGGGGGAAAGATATAATGAATGTTATAGCCTGTGAAGGCTGTGATAGGGTTCAGAGGCCAGAGACATATATGCATTGGCATTCTAGGCACATGGGAAATGGGTTTAGATCTTTGAAACTGGATAAGCAGATTATAAATAAATTGAAGGGTAAGTTAAGAGATGATGCATATAATAGTGATTTTCTGTTTGAGGTGAAGGAAAATTGGATGCTACAAGGTTGGAAGGGTAGAATTCTATTTGGTTCTTCTTGCTGGGTTCCAGCATAG
Protein sequence

>Msa0264470

MDKRVVGPHHEQSKTEFSSTVLGFINKFLMEDDDIEEDYNMFQHNEHEESSNALGYINQFLMEEDFEKEYTMFQDSYALQLTEKSFHDVISHPPPTAATAAAAAAAAAATLPSSSSSSSSYSSSSSIQHQQNYNYVHSPSFSDYSFSSGSTSSYELDPIIDSYNYNNNPFLLPTTLPSPPNHIFSQSNPTLFPSFNNALSHEVIKTENFEEEHFLNVSKQVNVDDNSELSELFDKLLLGTKVAKGPLQNTSFQQNEELSNRFGGFRRKRSYEEMDKGVVDPLHEQSKSEFTSTVLGFINKFLMEDDDIEEDYNMFQHNDHEESSNALGYINQFLMEEDFEKEYTMFQDSYALQLTEKSFHDVIAHTPLPPLPPTAAAAAVTAFTTTLPSSIQHEYQQNYNFVHSPNFSDYSLSSDSTTSFELDPHIDSYNYNYNPFLLPNTPPFSPNNFVSQSTSTFFPSFNNALSHELLQTENFEEEHFLNVSQNGSEQVYVDDSNELSELFDKVLVLGTKFTKGPLQNTSFQQNEELSNRFYGSRRQRSYEEVVDLRTLLMLCAQSISCNDISNANQILNQIKTHSSPTGDGTQRLAHFFGNALEARLAGTGSHIYRALSSKKKSAADMVKAYQVYSSACPFEKLAIMFSNDAILNVAKETESLHIIDFGVGYGFKWLGFIYRLSKRSGGPPKLRITGIDLPNSLERVNETGLRLASYCKRFNVPFEYNGIAKNWESIKVQDFKIRKNEFVAVTCVFKFENLPDETVVSENPRGAVLDLIKKANPNIFIHSIVNGGYDAPFFVTRFKEAVFYYSALFDMLDNNNVECEDPVRLMFEEDVWGKDIMNVIACEGCDRVQRPETYMHWHSRHMGNGFRSLKLDKQIINKLKGKLRDDAYNSDFLFEVKENWMLQGWKGRILFGSSCWVPA*